CN101865851A - Rapid lactic acid detection card - Google Patents
Rapid lactic acid detection card Download PDFInfo
- Publication number
- CN101865851A CN101865851A CN 201010125206 CN201010125206A CN101865851A CN 101865851 A CN101865851 A CN 101865851A CN 201010125206 CN201010125206 CN 201010125206 CN 201010125206 A CN201010125206 A CN 201010125206A CN 101865851 A CN101865851 A CN 101865851A
- Authority
- CN
- China
- Prior art keywords
- test paper
- lactic acid
- detection card
- acid detection
- housing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a rapid lactic acid detection card, which comprises a shell, a test paper system, a solution system and a solution adding mechanism. The shell consists of an upper cover and a lower cover. A blood sampling hole, a solution hole and a completion hole are arranged on the shell. A receiving pad is arranged in the shell. The test paper system comprises a set of solution guide test paper, reaction test paper and color development test paper which are arranged in the chamber of the shell. The main component contained in the solution system is peroxide enzyme solution. Since the invention integrates a blood filtration technology, an enzyme reaction technology and a chromatographic color development technology, the invention has the advantages that the instant determination of lactic acid in blood can be realized, the structure is simple, the supportive instruments are not required, the use is convenient, the cost is low and the detection card is compliant with the application trend of POCT (point of care testing).
Description
Technical field
The present invention relates to a kind of lactic acid detection card, especially relate to a kind of rapid lactic acid detection card of measuring lactic acid content in the blood.
Background technology
Lactic acid (lactic acid) is the end-product of glycolytic pathway.When the body anoxic, cell generates ATP by anaerobic metabolism.For the enough NAD of energy regeneration, carbohydrate metabolism is continued, lactic dehydrogenase is reduced to lactic acid by the pyruvic acid that glycolytic pathway is produced, and makes the NADH oxidation.The lactic acid of muscle accumulation is released in the blood circulation during histanoxia, passes through liver metabolism.
The normal value of serum lactic acid is 1.0 ± 0.5mmol/L, all can be considered normally less than 2mmol/L, can be diagnosed as " hyperlactacidemia " greater than this value.Lactic acid raises and sees anoxic and low perfusion states such as diabetic ketoacidosis, kidney failure, circulatory failure.The order of severity of dlactic acid mass formed by blood stasis is often pointed out the seriousness of illness, and when lactate level 10.5mmol/L, survival rate only has 30%.Raise for unaccountable metabolic acidosis of blood gas analysis and anion gap, must consider to detect lactic acid, inhale a large amount of propylene glycol of people and cause lactic acidosis.
Lactic acid extensively applies to clinical medicine, and the delivery room is on-the-spot to be detected, and in time finds and diagnose in utero anoxic of neonate; Emergency treatment scene, emergency case's vim and vigour detect; Operative site, the index of anesthesia patient's oxygen supply and acidosis monitoring; Before the sick bed, monitoring of the acidosis disease of intravenous hyperalimentation and glycogen disease patient's metabolism monitoring; In the hospital, hypertension and diabetes chronic diseases people's motion monitoring.Be widely used in sporting world's (sports medical science) simultaneously and judge lactic acid metabolism state in the personalized body, science is selected physical culture and sports personnel; Adopt an interval property lactic acid more in the motion and measure, for scientific and reasonable strength training provides important evidence.The environment of anti-lactic acid in player's body was trained in guidance, provided objective condition for improving sports achievement.In recent years, also be applied to medical science of recovery therapy.
The lactic acid assay method has chemical method and enzyme process, the chemical method poor accuracy, operate miscellaneous, can not robotization.Enzyme process has dehydrogenation enzyme process and oxidation enzyme process, the colourimetry of dehydrogenation enzyme process ultraviolet method and coupled tetrazolium, and also there are problems in the dehydrogenation enzyme process, can not solve.NAD is reduced into NADH when with lactic dehydrogenase lactic acid being oxidized to pyruvic acid, but needs coupling ALT and glutamic acid that the substrate pyruvic acid is removed with accelerated reaction, or coupling PMS and NBT colorimetric estimation, but the reaction endless.The Lactate Oxidase method is highly sensitive, degree of accuracy and accuracy are all fine, be fit to analyze automatically, but the problem that this method exists is to need to use special devices such as large-scale seralyzer or ultraviolet spectrophotometer, measure that required blood volume is more, the time is longer, cost an arm and a leg, be adapted at carrying out in large hospital or the laboratory.
The lactate detection test paper of special devices such as Biochemical Analyzer not needing to have appearred in recent years, for example the patent No. is that 03129094.9 Chinese patent discloses a kind of full blood lactic concentration and detects test paper, light source irradiation chromatic zones with wavelength 590nm, with the reflected light of photoelectric commutator reception from chromatic zones, the depth of catoptrical intensity and color is inversely proportional to, and the depth of color is reacted concentration of lactic acid in the tested blood sample.The patent No. is that 200410048302.2 Chinese patent discloses a kind of full blood lactic and detects strip, it is a kind of electrochemica biological sensor, it does substrate with an insulating substrate, a pair of metallic film conductive strips parallel to each other are vertically arranged in substrate, one end of two conductive strips is an incoming end, is covered with spacer on two conductive strips; Spacer and substrate be with wide, but be shorter than substrate, to expose two incoming ends; A groove is arranged on the spacer, and exposed portions serve insulating substrate and two parts conductive strips on it, two parts conductive strips that expose form working electrode and to electrode, be covered with reaction reagent layer at working electrode with on to electrode, insulating substrate on affixed one on the spacer, itself and spacer are with wide, and last insulating substrate covers the groove on the spacer, surround the uncovered kapillary cavity in two ends, cavity is the reaction zone of sensor.The reaction zone of this invention can be fixed the volume of detected blood under the situation that does not need manual intervention, and micro blood is entered and the reaction reagent reaction fast, measures the content of lactic acid in the blood at short notice.Though these lactate detection test paper do not need supporting special device, but still need supporting small-sized optical detection or sensing detection device, production cost is higher, and price is expensive.
Summary of the invention
Technical matters to be solved by this invention be for patient at home, mini clinic provide a kind of need not to rely on any device, easily and fast, the cheap rapid lactic acid detection card of measuring blood lactic acid content.
The technical scheme that the present invention is taked for its technical matters of solution is: a kind of rapid lactic acid detection card, comprise: housing, delivery system, solution system, liquid feeding mechanism, it is characterized in that: housing comprises loam cake and lower cover, and housing is provided with the blood sampling hole, is provided with in the housing and receives pad; Delivery system comprises that one is placed on and draws liquid test paper, reaction test paper and colour developing test paper in the housing cavity; The principal ingredient of contained solution is the superoxide enzyme solutions in the solution system.
Can display result in order to make when measure finishing, described housing proximal portion is provided with the end indicator hole, contains water-soluble end point indicator on the colour developing test paper of indicator hole below.
In order to obtain blood plasma by filtering whole blood, be coated with two-layer at least screen pack on the described blood sampling hole.
Described reception is lined with two working positions, when receiving pad and be in the blood sampling position, receive pad be positioned at the blood sampling hole under; When the reception pad was in the chromatography position, described reception pad was positioned at the below of drawing liquid test paper and reaction test paper, and its two ends are undertaken in the both sides test paper, and overlapping 0.5mm～1.5mm; Blood plasma volume 〉=10 μ L that described reception pad quantitatively absorbs.
Include Lactate Oxidase on the described reaction test paper, the concentration of Lactate Oxidase is 〉=2U/cm
2
In order conveniently to read desired result, the upper shell of described colour developing test paper top is provided with view window, and the view window subscript is provided with scale mark, and scale marker is arranged.
Described peroxidase concn 〉=0.6U/mL.
For solution system uses conveniently, described solution system places in the housing chamber or outside the housing chamber.
Liquid feeding for convenience, described liquid feeding mechanism is included in the sliding part that is provided with band acupuncture portion in the housing.
Because the technique scheme utilization, the present invention compared with prior art has following advantage:
1, the present invention combines blood filtration, enzyme reaction and chromatography developing technology, therefore can realize the instant mensuration to the lactic acid in the blood.
2, the present invention is simple in structure, need not use by necessary instrument, and easy to use, cost is low, meets the application trend of POCT (point of care testing, the instant detection).
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention one;
Fig. 2 is the rear view of the embodiment of the invention one;
Fig. 3 is the synoptic diagram of the embodiment of the invention one after opening loam cake;
Fig. 4 is the testing result figure in the embodiment of the invention one.
1, loam cake 2, blood sampling hole 3, solution hole 4, view window 5, end indicator hole 6, lower cover
7, sliding part 8, receive pad 9, draw liquid test paper 10, reaction test paper 11, colour developing test paper
Embodiment
Below in conjunction with drawings and Examples the present invention is further described:
Embodiment one
As Fig. 1, Fig. 2 and shown in Figure 3, a kind of rapid lactic acid detection card comprises: housing, delivery system, solution system, liquid feeding mechanism, and housing comprises loam cake 1 and lower cover 6, housing is provided with blood sampling hole 2, solution hole 3 and finishes indicator hole 5, is provided with the sliding part 7 that receives pad 8 and band spine in the housing; Delivery system comprises that one is placed on and draws liquid test paper 9, reaction test paper 10 and colour developing test paper 11 in the housing cavity; The principal ingredient of contained solution is the superoxide enzyme solutions in the solution system, peroxidase concn 〉=0.6U/mL, described solution system can place in the housing cavity or housing cavity outside.Be provided with a sliding part 7 that has an acupuncture portion in housing, pulling sliding part 7 punctures the solution bag, discharges solution and contacts or add a solution filling orifice with drawing liquid test paper 9, and the solution in the solution conduit is injected by filling orifice, and draws liquid test paper 9 and contacts.
Be coated with two-layer at least screen pack on the described blood sampling hole 2, but filtering whole blood obtains blood plasma; Upper shell corresponding to colour developing test paper 11 tops is provided with view window 4, and view window 4 subscripts are provided with scale mark, and scale marker is arranged; Described housing proximal portion is provided with and finishes indicator hole 5, contain water-soluble end point indicator on the colour developing test paper 11 of end indicator hole 5 belows, after 11 colour developings of colour developing test paper finish, the surplus solution chromatography is to finishing indicator hole 5 places, finish indicator hole 5 places and the indicator color will occur, show that detection has finished and can carry out the result to read.
Described reception pad 8 is provided with two working positions, blood sampling position and chromatography position, when receiving pad 8 and be in the blood sampling position, receive pad 8 be positioned at the hole 2 of taking a blood sample under; When reception pad 8 was in the chromatography position, described reception pad 8 was positioned at the below of drawing liquid test paper 9 and reaction test paper 10, and its two ends are undertaken in the both sides test paper, and overlapping 1mm, and the blood plasma volume quantitative that this reception pad 8 is absorbed is 15 μ L;
The preparation method of described reaction test paper 10 is: according to the form below formulated solution, test paper dipped in gets enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings.
The preparation method of described colour developing test paper 11 is: adopt hydrogen bromide or CDI activated carrier, treat the preferred N-alkyl benzene of the reagent amine material of Covalent Immobilization, this reagent is with developping solution diffusion, the preferred 2-20mmol/L of concentration after fixing.Color Appearance System also comprises another component: benzothiazolone hydrazone class, the preferred 0.01-1mg/mL of concentration.
The course of work of rapid lactic acid detection card is: when droplets of whole blood was added to blood sampling hole 2 on the housing, blood sampling hole 2 can be filtered whole blood obtain blood plasma; Blood plasma is received pad 8 and quantitatively absorbs; Solution in the solution box discharges, and draws liquid test paper 9 and by capillary action solution chromatography is successively filled up 8 to receiving, reacts test paper 10 and colour developing test paper 11; Colour developing test paper 11 presents the bluish violet colour band of certain altitude, by concentration of lactic acid in the colour band height indication whole blood.
The using method of rapid lactic acid detection card is:
1. placing flat test card punctures finger with blood taking needle, drips one to two and bleeds in the blood sampling hole 2, and blood the scale mark in the hole 2 of not taking a blood sample, receive fill up 8 be positioned at the hole 2 of taking a blood sample under, the blood plasma of quantitative collection 15 μ L;
2. wait for 2 minutes, the pulling shifting block will receive pad 8 and place the chromatography position, start reaction;
3. wait for 10-12 minute, reaction finished when indicator turned green in finishing indicator hole 5;
4. read the colour developing height, finding out corresponding lactic acid concn in the table of comparisons as a result.
People's matrix calibration serum lactate detection result, as following table and shown in Figure 4:
Claims (10)
1. rapid lactic acid detection card, comprising: housing, delivery system, solution system, liquid feeding mechanism is characterized in that:
Housing comprises loam cake (1) and lower cover (6), and housing is provided with blood sampling hole (2), solution hole (3) and finishes indicator hole (5), is provided with in the housing and receives pad (8);
Delivery system comprises that one is placed on and draws liquid test paper (9), reaction test paper (10) and colour developing test paper (11) in the housing cavity;
The principal ingredient of contained solution is the superoxide enzyme solutions in the solution system.
2. rapid lactic acid detection card according to claim 1 is characterized in that: described housing proximal portion is provided with and finishes indicator hole (5), contains water-soluble end point indicator on the colour developing test paper (11) of end indicator hole (5) below.
3. rapid lactic acid detection card according to claim 1 is characterized in that: be coated with two-layer at least screen pack on the described blood sampling hole (2).
4. rapid lactic acid detection card according to claim 1 is characterized in that: described reception pad (8) is provided with two working positions, blood sampling position and chromatography position, when receiving pad (8) and be in the blood sampling position, receive pad (8) be positioned at the hole (2) of taking a blood sample under; When reception pad (8) was in the chromatography position, described reception pad (8) was positioned at the below of drawing liquid test paper (9) and reaction test paper (10), and its two ends are undertaken in the both sides test paper, and overlapping 0.5mm～1.5mm; Blood plasma volume 〉=10 μ L that described reception pad (8) quantitatively absorbs.
5. according to the described rapid lactic acid detection card of claim 1, it is characterized in that, include the Lactate Oxidase composition on the described reaction test paper (10).
6. rapid lactic acid detection card according to claim 5 is characterized in that: the concentration of described Lactate Oxidase is 〉=2U/cm
2
7. rapid lactic acid detection card according to claim 1 is characterized in that: the upper shell of colour developing test paper (11) top is provided with view window (4).
8. according to the described rapid lactic acid detection card of claim 1, it is characterized in that: described peroxidase concn 〉=0.6U/mL.
9. rapid lactic acid detection card according to claim 1 is characterized in that: described solution system places in the housing chamber or outside the housing chamber.
10. rapid lactic acid detection card according to claim 1 is characterized in that: described liquid feeding mechanism is included in the sliding part (7) that is provided with band acupuncture portion in the housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010125206 CN101865851A (en) | 2010-03-16 | 2010-03-16 | Rapid lactic acid detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010125206 CN101865851A (en) | 2010-03-16 | 2010-03-16 | Rapid lactic acid detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101865851A true CN101865851A (en) | 2010-10-20 |
Family
ID=42957655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010125206 Pending CN101865851A (en) | 2010-03-16 | 2010-03-16 | Rapid lactic acid detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101865851A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434321A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Microbial thallus microfiltration detection panel |
| CN106770187A (en) * | 2016-11-21 | 2017-05-31 | 东南大学 | A kind of electrochromic devices array and its preparation, application method |
| CN110426357A (en) * | 2019-08-07 | 2019-11-08 | 杭州皓阳生物技术有限公司 | A kind of lactate detection reagent and its detection method |
| CN111175292A (en) * | 2020-01-20 | 2020-05-19 | 杭州联晟生物科技有限公司 | Test strip for detecting lactic acid and preparation method thereof |
| CN112875017A (en) * | 2021-01-25 | 2021-06-01 | 上海快灵生物科技有限公司 | Test paper card box and test paper card |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456893A (en) * | 2003-06-06 | 2003-11-19 | 浙江大学 | Whole blood lactic acid concentration dectecting paper strip |
| CN101509925A (en) * | 2009-03-02 | 2009-08-19 | 苏州市玮琪生物科技有限公司 | Chromatography type total cholesterol self-measuring instrument |
-
2010
- 2010-03-16 CN CN 201010125206 patent/CN101865851A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456893A (en) * | 2003-06-06 | 2003-11-19 | 浙江大学 | Whole blood lactic acid concentration dectecting paper strip |
| CN101509925A (en) * | 2009-03-02 | 2009-08-19 | 苏州市玮琪生物科技有限公司 | Chromatography type total cholesterol self-measuring instrument |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770187A (en) * | 2016-11-21 | 2017-05-31 | 东南大学 | A kind of electrochromic devices array and its preparation, application method |
| CN106434321A (en) * | 2016-11-23 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Microbial thallus microfiltration detection panel |
| CN110426357A (en) * | 2019-08-07 | 2019-11-08 | 杭州皓阳生物技术有限公司 | A kind of lactate detection reagent and its detection method |
| CN111175292A (en) * | 2020-01-20 | 2020-05-19 | 杭州联晟生物科技有限公司 | Test strip for detecting lactic acid and preparation method thereof |
| CN112875017A (en) * | 2021-01-25 | 2021-06-01 | 上海快灵生物科技有限公司 | Test paper card box and test paper card |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7347973B2 (en) | Synchronized analyte testing system | |
| US7004901B2 (en) | Method and kit for the transdermal determination of analyte concentration in blood | |
| EP1038176B1 (en) | Embossed test strip system | |
| US9883823B2 (en) | System and method for determining an in vivo concentration of a small molecule substance of interest in a noninvasive manner | |
| JPH04223267A (en) | Nonreturnable reagent unit | |
| Yue et al. | Simple, skin-attachable, and multifunctional colorimetric sweat sensor | |
| CN101865851A (en) | Rapid lactic acid detection card | |
| US9835613B2 (en) | Rapid small volume detection of blood ammonia | |
| US20220178868A1 (en) | Means for the quantitative determination of cationic electrolyte concentration and creatinine concentration and of their ratios | |
| CN101865853A (en) | Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof | |
| CN1912626A (en) | Preparation of disposable carbon-base glucose chip and electrochemical detection method | |
| Woo et al. | The advance of technology as a prelude to the laboratory of the twenty-first century | |
| US20030077205A1 (en) | Diagnostic test optical fiber tips | |
| CN202814993U (en) | Palm-type rapid quantitative detector of glycosylated hemoglobin | |
| Urban et al. | CO2 sensing in medicine | |
| CN213302245U (en) | Semi-automatic cover closing mechanism of dry biochemical analyzer | |
| CN217931512U (en) | Dry-type three-electrode electrochemical luminescence chip | |
| EP4353829A1 (en) | Method for quantifying phenylalanine in blood, and measurement kit used therefor | |
| Nagamine et al. | Wet Interface Technologies for Wearable Sweat Sensors | |
| US20040229347A1 (en) | Embossed test strip system | |
| CN117288864A (en) | Chiral resolution method of DL-lactic acid and application of chiral resolution method in detection method of DL-lactic acid in human sweat | |
| Hook et al. | Blood glucose monitors: technology for home healthcare | |
| US8501111B2 (en) | Small volume and fast acting optical analyte sensor | |
| Morgan | Development of a micromachined optoelectronic carbon dioxide sensor for transcutaneous monitoring of neonates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: GU YU Free format text: FORMER OWNER: SUZHOU WEIQI BIOLOGY SCIENCE AND TECHNOLOGY CO., LTD. Effective date: 20110713 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20110713 Address after: 215011 No. 6 Jin Shan Road, Suzhou hi tech Development Zone, Jiangsu, China Applicant after: Gu Yu Address before: 215011 No. 6 Jin Shan Road, Suzhou hi tech Development Zone, Jiangsu, China Applicant before: Suzhou Weiqi Biology Science and Technology Co., Ltd. |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20101020 |