CN101810643A - Goose gall extract, preparation method and medicine use thereof - Google Patents
Goose gall extract, preparation method and medicine use thereof Download PDFInfo
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Abstract
The invention relates to a goose gall extract, a preparation method and medicine use thereof, which belong to the field of traditionally Chinese medicine. The preparation method comprises the following steps: taking goose galls; removing gall coats; carrying out filtration; collecting filter liquid; adding 50 to 90 percent edible alcohol to the concentration of 50 to 90 percent; carrying out precipitation for 5 to 48 hours at 2 to 24 DEG C; carrying out filtration; collecting filter liquid to obtain goose bile raw liquid; recovering alcohol; and carrying out concentration and drying to obtain the goose gall extract. The invention also provides the application of the goose gall extract of the invention to the preparation of medicine for clearing away heat and toxic materials, protecting the liver and improving the immunity. The goose bile prepared through the modern extraction technology by the invention has the obvious function of activating and enhancing the reticulo endothelial system of mice, can improve the organism nonspecific immunity function, and has the effects of clearing away heat and toxic materials, protecting the liver and improving the immunity.
Description
Technical field
The invention belongs to the field of Chinese medicines, especially refer to goose gall extract and preparation method thereof and medicinal usage thereof.
Background technology
Bile is in China's application pharmaceutically, and is with a long history.As far back as the Eastern Han Dynasty, just put down in writing a kind of Fel Cyprinus carpio in China's first pharmacy monograph Shennong's Herbal.Ancient times, famous physician's Zhang Zhongjing was put down in writing Fel Sus domestica treatment disease in treatise on Febrile Diseases.31 kinds have been recorded in the Compendium of Material Medica of Ming Dynasty's Li Shizhen (1518-1593 A.D.).2 kinds have been increased income in " Compendium of Materia Medica is lifted something lost " of Qing Dynasty's ZHAO Xue-Min.Put down in writing 35 kinds in modern new " the Chinese medicine voluminous dictionary " published, all listed " Chinese medicine voluminous dictionary " (Shanghai science tech publishing house, 2006.3) in as Fel Gallus domesticus, Fel Anseris domestica, Fel Bovis seu Bubali, Fel Caprae seu Ovis, Fel Sus domestica, Fel Canitis, Fel Ursi etc.The composition of " Chinese medicine voluminous dictionary " year goose bile is similar to Fel Gallus domesticus, mainly contains chenodeoxycholic acid (CDCA) and cholic acid (CA) etc.CDCA has function of gallbladder promoting, dissolving cholelithiasis, promotes fat digestion and absorption, and eliminating phlegm and stopping cough is relievingd asthma, blood fat reducing, resisting hypertension and effect such as antibiotic.Effects such as CA has that maincenter inhibition, heart tonifying, blood pressure lowering, function of gallbladder promoting, eliminating phlegm and stopping cough are relievingd asthma, antiinflammatory and antiallergic, antibiotic and antiviral.But the modern study about Fel Anseris domestica is few.
Summary of the invention
The invention provides a kind of goose gall extract and preparation method thereof and medicinal usage thereof, be used for heat-clearing and toxic substances removing, protect the liver, improve immunity.Goose gall extract of the present invention is obtained by following method:
Get bright Fel Anseris domestica, remove the gallbladder skin after, filter, collect filtrate, add the 50-95% edible ethanol to 50-70% concentration, 2-24 ℃ of precipitation 5-48 hour is down filtered, and collects filtrate and gets goose bile stock solution, reclaims ethanol, concentrate, drying, goose gall extract.
Goose gall extract of the present invention is preparing heat-clearing and toxic substances removing, is protecting the liver, improving the application in the immune medicine.
The present invention has obvious activation with the goose bile that modern extraction process makes, and strengthens the reticuloendothelial system function of mice, can improve the body non-specific immunity, heat-clearing and toxic substances removing is arranged, protect the liver, improve immunity effect is arranged.The present invention is oral: every bottle of 5ml, oral one time one bottle, 3 times on the one.
The specific embodiment
Embodiment 1
Get bright Fel Anseris domestica, remove the gallbladder skin after, filter, collect filtrate, add 50% edible ethanol to 50% concentration, 2 ℃ of precipitations 5 hours are down filtered, and collect filtrate and get goose bile stock solution, reclaim ethanol, concentrate, drying, goose gall extract.
Embodiment 2
Get bright Fel Anseris domestica, remove the gallbladder skin after, filter, collect filtrate, add 75% edible ethanol to 60% concentration, 13 ℃ of precipitations 25 hours are down filtered, and collect filtrate and get goose bile stock solution, reclaim ethanol, concentrate, drying, goose gall extract.
Embodiment 3
Get bright Fel Anseris domestica, remove the gallbladder skin after, filter, collect filtrate, add 95% edible ethanol to 70% concentration, 24 ℃ of precipitations 48 hours are down filtered, and collect filtrate and get goose bile stock solution, reclaim ethanol, concentrate, drying, goose gall extract.
Further specify drug effect of the present invention below in conjunction with pharmacodynamic experiment.
Test material
One, the medicine goose gall extract is obtained by embodiment 2 methods, the positive control drug liver-protecting tablet is produced every heavy 0.35g (containing extract 0.25g/ sheet) by Shiyitang Pharmaceutic Plant, lot number 011128 is prepared desired concn with distilled water during test, and matched group gives the equal-volume distilled water.
Two, animal mice, the Kunming kind, male and female half and half, every group of 12-15 of body weight 25 ± 2g only provided by the breeding of academy laboratory animal room of Shanxi Province institute of traditional Chinese medicine.
Three, other materials is routine and chooses.
Method and result
One, to the protective effect of mouse carbon tetrachloride acute liver damage
Mice is divided into six groups at random, sets up blank group, carbon tetrachloride model group, the high, medium and low dosage group of goose gall extract and positive drug liver-protecting tablet matched group separately.Each administration group is gastric infusion (blank group, model group are given consubstantiality hydrops), once a day, preceding 10 hours of successive administration, except that the blank group, all the other respectively organize equal lumbar injection 0.2%CCl
4Soybean oil solution 0.2ml/20g, 20 hours thereafter, get blood by the eyeground vein clump, separation of serum is surveyed serum alanine aminotransferase (ATL) with colorimetry, glutamic oxaloacetic transaminase, GOT (COT) content, the result carries out statistical procedures.Simultaneously each treated animal is put to death, get liver, mirror is observed each treated animal hepar damnification degree down, sees Table 1.
Table 1 goose gall extract is to CCl
4The influence of poisoning mice Serum ALT GOT content
| Group | Number of animals (only) | Dosage (ml/kg) | ??ALT??(X±SD，u) | ??GOT??(X±SD，u) |
| Blank | ??15 | ??47.8±11.2** | ??59.3±14.9* | |
| The model contrast | ??14 | ??106.9±25.6 | ??76.8±15.7 | |
| The goose gall extract height | ??14 | ??20.0 | ??80.3±21.7** | ??72.4±17.6 |
| In the goose gall extract | ??15 | ??10.0 | ??83.5±20.9* | ??67.4±20.5 |
| Goose gall extract is low | ??13 | ??5.0 | ??87.1±19.0* | ??73.1±17.9 |
| Liver-protecting tablet | ??15 | ??2.0 | ??85.4±21.3* | ??69.3±11.3 |
Annotate: compare * * P＜0.01 * P＜0.05 with model group
Table 1 is the result show, model group is to all obviously risings (comparing with the blank group) of Serum ALT, GOT behind the carbon tetrachloride.And each administration group is compared with model group, and Serum ALT content all obviously reduces, but GOT is not had obvious influence.In addition, it is basic identical that the dosage group is compared (same dose) reduction glutamate pyruvate transaminase intensity in the goose gall extract with the liver-protecting tablet group, there was no significant difference.
Observe down through mirror, blank group lobules of liver structural integrity, karyon circle, clear, intact nuclear membrane, hepatocyte is that the center is radial arrangement with the central vein, there is no unusually, model group lobules of liver structure disturbance, hepatocyte is seriously downright bad, most of karyolysis, mitochondrial swelling, substrate is loose, occurs a large amount of cavitys in the endochylema.Each group of goose gall extract and liver-protecting tablet group are compared with model, the lobules of liver structure disturbance, and degree is basic identical with model group, and the hepatic necrosis degree alleviates to some extent, and mitochondrial swelling degree and substrate porousness also alleviate to some extent.
Experimental result shows, the protective effect of the mouse liver injury that goose gall extract and former dosage form cause carbon tetrachloride.
Two, to the protective effect of D-galactosamine hydrochlorate acute liver damage
Animal grouping, dosage, administration time are all with experiment one.Cause liver injury medicament and select the D-galactosamine hydrochlorate for use, mice is pressed 600mg/kg lumbar injection (10% concentration, pH value are 7.0), inject back 30 hours each treated animal and get blood, survey alaninyl transferring enzyme and glutamic oxaloacetic transaminase, GOT content in the serum, the result carries out statistical procedures, sees Table 2.
Table 2 goose gall extract is to the influence of D-galactosamine poisoning mice Serum ALT, GOT
| Group | Number of animals (only) | Dosage (g/kg) | ??ALT??(X±SD，u) | ??GOT??(X±SD，u) |
| Blank | ??12 | ??45.1±8.7** | ??59.5±17.6* | |
| The model contrast | ??13 | ??90.3±25.8 | ??81.1±27.5 | |
| The goose gall extract height | ??14 | ??20.0 | ??57.2±15.6** | ??71.4±20.1 |
| In the goose gall extract | ??12 | ??10.0 | ??61.0±19.7** | ??69.5±21.6 |
| Goose gall extract is low | ??13 | ??5.0 | ??63.9±20.1* | ??74.5±19.9 |
| Liver-protecting tablet | ??13 | ??2.0 | ??63.3±21.4* | ??70.5±21.3 |
Annotate: compare * * P＜0.01 * P＜0.05 with model group
Experimental result shows that model group is compared with the blank group, and Serum ALT obviously raise after mice gave D-galactosamine, and GOT also has rising trend, but not obvious.Each administration group is compared with model group respectively, and ALT is obviously reduced, and goose gall extract is certain dose-effect relationship, and the dosage group is compared no explicitly difference in the goose gall extract with the liver-protecting tablet group, and it is basic identical that both reduce the ATL effect.In addition, each administration group does not all have tangible influence to GOT.
Three, to the influence of mouse monokaryon phagocytic function
Get 72 of mices, be divided into six groups at random, 12 every group, male and female half and half.Except that the blank group, all the other six groups equal lumbar injection hydrocortisone 25mg/kg, each administration group is pressed table 3 dosage gastric infusion simultaneously, once a day, successive administration 7 days (matched group, model group are given consubstantiality hydrops).After the last administration 3 hours, by tail vein injection india ink 1ml/kg, injected back 1 minute, got blood 20ul from the eye socket vein respectively, and be dissolved in 2ml0.1%Na in 5 minutes
2CO in the solution, measures trap under wavelength 650nm.At last mice is put to death, claim to decide liver, spleen weight, calculate phagocytic index K value and activate the phagocytic capacity a value, the result carries out statistical procedures, sees Table 3:
Table 3 goose bile is to the influence of mouse monokaryon phagocytic function
| Group | Number of animals (only) | Dosage (g/kg) | Phagocytic index (K) (X ± SD) | Activate the phagocytic capacity (a) (X ± SD) |
| Blank | ??12 | ??0.0334±0.0079** | ??4.26±0.75** | |
| The model contrast | ??12 | ??0.0159±0.0049 | ??3.23±0.61 | |
| The goose gall extract height | ??12 | ??4.0 | ??0.0275±0.0098** | ??3.85±0.69* |
| In the goose gall extract | ??12 | ??2.0 | ??0.0294±0.0103** | ??3.69±0.78 |
| Goose gall extract is low | ??12 | ??1.0 | ??0.0235±0.0059* | ??3.75±0.59 |
| Liver-protecting tablet | ??12 | ??2.0 | ??0.0259±0.0083** | ??3.52±0.74 |
Annotate: compare * * P＜0.01 * P＜0.05 with model group
Experimental result shows, the same model group of blank group (hydrocortisone group) is compared, and K, a value are obviously reduced, and each administration group is compared with model group, can resist the K value that causes by hydrocortisone and reduce, and can reach normal level (comparing no explicitly difference) with the blank group.The goose bile high dose group obviously raises a value.
Four, heat clearing away effect
Test objective is observed the refrigeration function of goose bile to the heating animal
Obtained by embodiment 2 methods by reagent thing goose gall extract.
The purebred rabbit of animal New Zealand, sex: male, body weight: 1.6～2.2kg, 12 of every group of rabbit.
Experimental technique is selected to separate heat test in the selective body.With typhoid fever, pair typhoid fever first and second triple vaccinies is the pyrogenicity agent, the Lanzhou biochemical-pharmaceutical factory.Lot number: 020108
The test key step
Select body temperature to supply experiment to use 60 of 33 ℃～38.9 ℃ purebred rabbit of New Zealand.Being divided into 5 groups at random is three groups of normal saline group, goose gall extracts: high, medium and low dosage, aspirin group, ear vein injection triple vaccine 0.8ml/kg, after treating that animal heat rising difference reaches 0.8 ℃, goose bile is irritated stomach 5.4g/kg, 2.8g/kg, 1.4g/kg goose bile respectively for three groups, the aspirin group is irritated stomach 0.37g/kg aspirin, the normal saline group is irritated stomach 5ml/kg normal saline, surveyed the anus temperature every 1 hour once, surveyed the anus temperature and basic anus using warming therapy difference is the index of body temperature variation with different time.The animals administer afterreaction
Observation index: the anus temperature changes after the rabbit administration.
Observing time: administration timing in 30 minutes, observed altogether 5 hours, surveyed the anus temperature once every 1 hour.
Dosage is provided with: 3 dosage groups, heavy dose of group 4ml/kg, middle dosage group 2ml/kg, small dose group 1ml/kg.
[medication] gastric infusion, administration number of times 1 time.
[experimental control] blank is a normal saline.
Positive control medicine: aspirin, lot number 010802, Northwest Synthetic Pharmaceutics Factory No.2.Dosage 0.37g/kg.Chlorpromazine, lot number: 010425, Xi'an pharmaceutical factory.Dosage 25mg/kg.
[result of the test]
Three groups of goose gall extract 4ml/kg, 2ml/kg and 1ml/kg all produced remarkable cooling effect in 3 hours to the pyrogenicity rabbit after administration, compare significant difference (P＜0.05) with the normal saline group, significant difference (P＜0.01) is compared in the cooling effect of aspirin group with the normal saline group, its temperature-fall period is faster than goose bile, and effect is strong, and both compare significant difference (P＜0.01) and see Table 2.
Table 2 goose gall extract is to the influence of rabbit body temperature due to the pyrogenicity agent
| Group | Dosage | ??n | Basal body temperature | ??1h | ??2h | ??3h | ??4h | ??5h |
| The saline group | ??5ml/kg | ??12 | ??38.60±??0.13 | ??39.83±??0.34 | ??39.95±??0.26 | ??39.55±??0.24 | ??39.28±??0.25 | ??38.85±??0.30 |
| Goose gall extract | ??4ml/kg | ??12 | ??38.57±??0.16 | ??39.89±??0.45 | ??39.78±??0.40 | ??39.18±??0.30 ** | ??38.56±??0.22** | ??38.44±??0.21 ** |
| Goose gall extract | ??2ml/kg | ??12 | ??38.53±??0.13 | ??39.88±??0.44 | ??39.43±??0.45 ** | ??39.08±??0.30 ** | ??38.68±??0.28 ** | ??38.45±??0.24 ** |
| Goose gall extract | ??1ml/kg | ??12 | ??38.45±??0.19 | ??39.51±??0.41 | ??39.72±??0.43 | ??39.04±??0.32 * | ??38.74±??0.41 ** | ??38.44±??0.19 ** |
| Aspirin | ??0.37g/kg | ??12 | ??38.52±??0.29 | ??38.89±??0.50 ** | ??38.59±??0.62 ** | ??38.18±??0.55 ** | ??37.89±??0.55 ** | ??37.85±??0.56 ** |
Compare with the normal saline group
*P＜0.05
*P＜0.01
Conclusion
Under this experiment condition, two kinds of animals, two kinds of analgesic experiments of method show: goose bile can significantly reduce typhoid fever, pay the rising of rabbit body temperature due to typhoid fever first and second triple vaccinies, its 4ml/kg and 2ml/kg are analgesic effective dose but refrigeration function and speed are weaker than the aspirin of 0.37g/kg.The goose bile of 1ml/kg dosage does not have refrigeration function.
The The above results explanation, goose bile has obvious activation, strengthens the reticuloendothelial system function of mice, can improve the body non-specific immunity, and refrigeration function is arranged.
Preparation:
1, tablet: get goose gall extract 100g, add starch 60g, lactose 30g, hydroxypropyl cellulose 1.25g, make granule, drying, granulate adds Pulvis Talci 5g and magnesium stearate 0.5g mix homogeneously, makes 1000, the bag film-coat, promptly.Three times on the one, one time 2.
2, capsule: goose gall extract 100g is pulverized, add micropowder silica gel and the dextrin composite auxiliary material 100g by 4: 1 compositions, granulate, mixing incapsulates.Three times on the one, one time 2.
3, granule: get goose gall extract 100g, add starch 60g, sucrose 30g, hydroxypropyl cellulose 1.25g, make granule, drying, granulate, pack 1000g is promptly.Three times on the one, one time one bag.
Claims (3)
1. goose gall extract is characterized in that it is made by following method: get bright Fel Anseris domestica, remove the gallbladder skin after, filter, collect filtrate, add the 50-95% edible ethanol to 50-70% concentration, 2-24 ℃ precipitates 5-48 hour down, filter, collect filtrate and get goose bile stock solution, reclaim ethanol, concentrate, drying gets goose gall extract.
2. the preparation method of goose gall extract as claimed in claim 1.
3. goose gall extract as claimed in claim 1 is preparing heat-clearing and toxic substances removing, is protecting the liver, improving the application in the immune medicine.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106668069A (en) * | 2015-11-04 | 2017-05-17 | 张志强 | Bile extract as well as extracting method and application thereof |
| CN107184864A (en) * | 2017-05-23 | 2017-09-22 | 寿县向天歌白鹅开发有限公司 | A kind of goose courage Chinese medicine composition and preparation method thereof |
| CN115040545A (en) * | 2022-06-13 | 2022-09-13 | 江南大学 | Application of chicken gall powder in preparation of medicine for dispelling effects of alcohol and protecting liver |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3919266A (en) * | 1972-09-21 | 1975-11-11 | Intellectual Property Dev Corp | Production of bile acids |
| CN1357334A (en) * | 2001-04-01 | 2002-07-10 | 浙江青荷生化股份有限公司 | Artificial bear bile powder |
| CN101143886A (en) * | 2007-10-21 | 2008-03-19 | 刘永存 | Non-organic solvent extraction method for chenodeoxycholic acid in bird gall |
| CN101220074A (en) * | 2008-01-15 | 2008-07-16 | 吴夏君 | Method for extracting bile acid from animal bile |
-
2010
- 2010-05-07 CN CN2010101647951A patent/CN101810643B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3919266A (en) * | 1972-09-21 | 1975-11-11 | Intellectual Property Dev Corp | Production of bile acids |
| CN1357334A (en) * | 2001-04-01 | 2002-07-10 | 浙江青荷生化股份有限公司 | Artificial bear bile powder |
| CN101143886A (en) * | 2007-10-21 | 2008-03-19 | 刘永存 | Non-organic solvent extraction method for chenodeoxycholic acid in bird gall |
| CN101220074A (en) * | 2008-01-15 | 2008-07-16 | 吴夏君 | Method for extracting bile acid from animal bile |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106668069A (en) * | 2015-11-04 | 2017-05-17 | 张志强 | Bile extract as well as extracting method and application thereof |
| CN107184864A (en) * | 2017-05-23 | 2017-09-22 | 寿县向天歌白鹅开发有限公司 | A kind of goose courage Chinese medicine composition and preparation method thereof |
| CN115040545A (en) * | 2022-06-13 | 2022-09-13 | 江南大学 | Application of chicken gall powder in preparation of medicine for dispelling effects of alcohol and protecting liver |
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| CN101810643B (en) | 2011-10-05 |
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Inventor after: Li Pingya Inventor after: Liu Jianwei Inventor after: Liu Chuangui Inventor after: Jin Liqun Inventor after: Wang Fuwen Inventor after: Wang Wenjie Inventor after: Yan Zhili Inventor after: Zhang Guangmin Inventor before: Li Pingya Inventor before: Liu Chuangui Inventor before: Jin Liqun Inventor before: Wang Fuwen Inventor before: Wang Wenjie Inventor before: Yan Zhili Inventor before: Zhang Guangmin |
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Free format text: CORRECT: INVENTOR; FROM: LI PINGYA LIU ZHUANGUI JIN LIQUN WANG FUWEN WANG WENJIE YAN ZHILI ZHANG GUANGMIN TO: LI PINGYA LIU JIANWEI LIU ZHUANGUI JIN LIQUN WANG FUWEN WANG WENJIE YAN ZHILI ZHANG GUANGMIN |
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