CN101798597A - Assistant identification method of wheat with advanced characters - Google Patents
Assistant identification method of wheat with advanced characters Download PDFInfo
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Abstract
The invention discloses an assistant identification method of wheat with advanced characters. The method comprises the following steps of: using a gene group DNA of wheat to be tested as a template; using a primer pair comprising DNA shown by a sequence 3 of a sequence table and DNA shown by a sequence 4 of the sequence table for PCR amplification; and then, carrying out anyone of the following six judgments: (1) the thousand kernel weight of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics; (2) the grain diameter of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics; (3) the ear weight of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics; (4) the ear number of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics; (5) the flag leaf area of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics; and (6) the flag leaf width of wheat capable of amplifying DNA segments is smaller than that of wheat incapable of amplifying DNA segments on statistics. The method can be applied to wheat breeding, realizes early screening and saves time and resources.
Description
Technical field
The present invention relates to the method for wheat that a kind of assistant identification has good character.
Background technology
For a long time, wheat yield is the improvement of breeding man always and cultivates of paramount importance target in the kind, but output is affected by environment generally bigger, and especially the influence of water and fertilizer condition is bigger, and therefore cultivating the eurytropy kind then becomes the main utilization means that improve wheat yield.In recent years, along with the fast development of Protocols in Molecular Biology, a plurality of QTLs site relevant with yield traits also was found gradually and located.(Groos C such as Groos, Robert N, Bervas E, Charmet G.Genetic analysis of grain protein-content, grain yield andthousand-kernel weight in bread wheat.Theor.Appl.Genet.2003,106:1032-1040) on the 7D of wheat karyomit(e), find the QTL of control wheat yield, on karyomit(e) 7A and 7D, then found the QTL of control seed thousand seed weight.(Quarrie SA such as Quarrie, Steed A, Calestani C, et al.A high density genetic map of hexaploid wheat (Triticumaestivum L.) from the cross Chinese Spring x SQ1 and its use to compare QTLsfor grain yield across a range of environments.Theor.Appl.Genet.2005,110:865-880; Quarrie SA, Pekic Quarrie S, Radosevic R, et al.Dissecting a wheatQTL for yield present in a range of environments:from the QTL to candidategenes, J Exp.Botany 2006,57:2627-2637) then in a DH colony, found the QTL site of a plurality of control yield traitses, wherein the contribution of the QTL on 7AL and the 7BL is maximum, especially the contribution rate to wheat grains per spike is the highest, and he has also found the heavy QTL of control wheat groat on 7AL.
(Wilkinson M such as Wilkinson, Wan Y, Tosi P, et al.Identification and geneticmapping of variant forms of puroindoline b expressed in developing wheat grain.J Cereal Sci, 2008,48:722-728) found that in the cDNA of wheat three kinds reach 72% gene with puroindoline b gene similarity, and be located 7A chromosomal long-armed on, further analysis revealed, this gene may can be regulated and control about flint wheat SKCS hardness 5-7.(Chen F such as Chen, Brian B, Morris CF.Puroindoline b-2 in wheat.Theoretical and applied genetics, 2009, DOI10.1007/s00122-009-1195-y) above-mentioned three genes are named, be respectively Pinb-2v1, Pinb-2v2 and Pinb-2v3, and found a kind of new gene type, called after Pinb-2v4, utilize aneuploid to carry out finding after the physical positioning, Pinb-2v1 is positioned on the 7DL karyomit(e), Pinb-2v2 is positioned on the 7BL karyomit(e), and Pinb-2v3 is positioned on the 7B karyomit(e), and Pinb-2v4 then is positioned on the 7AL karyomit(e), and Pinb-2v1 and Pinb-2v4 appear in all kinds of investigation, and Pinb-2v2 then appears at respectively in the different kinds with Pinb-2v3.
Summary of the invention
The purpose of this invention is to provide the method for wheat that a kind of assistant identification has good character.
The invention provides assistant identification and have the various trait method of wheat, comprise the steps: that the genomic dna with wheat to be measured is a template, the primer of forming with DNA shown in the sequence 4 of DNA shown in the sequence 3 of sequence table and sequence table carries out following 1 then to carrying out pcr amplification) to 6) in arbitrary described judgement:
1) can amplify the thousand seed weight of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
2) can amplify the particle diameter of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
3) the fringe grain that can amplify the wheat of dna fragmentation focuses on the statistics less than the wheat that can not amplify dna fragmentation;
4) can amplify the grain number per spike of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
5) the boot leaf area of wheat that can amplify dna fragmentation at statistics less than the wheat that can not amplify dna fragmentation;
6) flag leaf width of wheat that can amplify dna fragmentation at statistics less than the wheat that can not amplify dna fragmentation.
1) to 6) in, described dna fragmentation specifically can be the dna fragmentation of 401bp.
The present invention also protects the primer that DNA forms shown in the sequence 4 of DNA shown in the sequence 3 of sequence table and sequence table right.
Described primer also belongs to protection scope of the present invention to the application in the test kit that has the various trait wheat in the preparation assistant identification; Described proterties is at least a in thousand seed weight, particle diameter, grain number per spike, every fringe grain heavy (the fringe grain is heavy), boot leaf area and the flag leaf width.
The present invention also protects a kind of assistant identification to have the test kit of various trait wheat, comprises that described primer is right; Described proterties is that thousand seed weight, particle diameter, grain number per spike, every fringe grain are heavy, at least a in boot leaf area and the flag leaf width.
Another kind of assistant identification provided by the invention has the various trait method of wheat, is to detect Nucleotide shown in the sequence 2 that whether has sequence table in the genomic dna of wheat to be measured, carries out following 1 then) to 6) in arbitrary described judgement:
1) thousand seed weight that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
2) particle diameter that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
3) the fringe grain that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna focuses on the statistics wheat less than Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
4) grain number per spike that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
5) the boot leaf area that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna does not have the wheat of Nucleotide shown in the sequence 2 of sequence table in less than genomic dna at statistics;
6) flag leaf width that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna does not have the wheat of Nucleotide shown in the sequence 2 of sequence table in less than genomic dna at statistics.
The third assistant identification provided by the invention has the various trait method of wheat, be to detect the sequence 2 that whether has sequence table in the genomic dna of wheat to be measured, carry out following 1 then from Nucleotide shown in the 34th to 434 at the 5 ' end) to 6) in arbitrary described judgement:
1) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the thousand seed weight of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
2) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the particle diameter of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
3) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in the fringe grain of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end focuses on the statistics less than genomic dna is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
4) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the grain number per spike of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
5) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in less than genomic dna at statistics from the boot leaf area of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
6) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in less than genomic dna at statistics from the flag leaf width of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end.
Genomic dna with wheat to be measured is a template, and the primer of forming with DNA shown in the sequence 4 of DNA shown in the sequence 3 of sequence table and sequence table is sequence table to the dna fragmentation that carries out pcr amplification and obtain sequence 2 is from DNA shown in the 34th to 434 at the 5 ' end.
Described method, described primer is right, and described test kit all can be applicable to wheat breeding.
The present invention also protects a kind of protein, is following (a) or (b):
(a) protein of forming by the aminoacid sequence shown in the sequence in the sequence table 1;
(b) with the aminoacid sequence of sequence 1 through the replacement of one or several amino-acid residue and/or disappearance and/or interpolation and relevant with the Wheat Production proterties by sequence 1 deutero-protein.
Described proteinic encoding gene also belongs to protection scope of the present invention.
Described encoding gene specifically can be following 1) or 2) or 3) or 4) dna molecular:
1) dna molecular shown in the sequence 2 in the sequence table;
2) sequence 2 is held the dna molecular shown in the 34th to 434 Nucleotide from 5 ' in the sequence table;
3) under stringent condition with 1) or 2) the dna sequence dna hybridization that limits and the dna molecular of coding Wheat Production character-related protein;
4) with 1) or 2) or 3) dna sequence dna that limits has 90% above homology and the dna molecular of the Wheat Production character-related protein of encoding.
The invention discloses a function and an application in the wheat yield character improvement thereof with wheat yield trait related gene Pinb-2v2, the wheat breed that contains the Pinb-2v2 gene mainly shows as with the closely-related proterties aggregate performance of yield traits bad, is embodied in respectively that thousand seed weight is low, particle diameter is little, the fringe grain is heavily low, grain number per spike is few, boot leaf is narrower and characteristics such as the boot leaf area is less.Heavy mean value 1.86 grams of common wheat kind thousand seed weight mean value 40.3 grams, 2.36 millimeters of particle diameter mean values, 45.5 of grain number per spike mean values, every fringe grain, 25.2 square centimeters of boot leaf area average, 1.72 centimetres of flag leaf width mean values with Pinb-2v3 gene.Therefore, by reticent Pinb-2v2 gene, eliminate its negative impact and will also help the heredity and the molecular biological further research of yield traits simultaneously utilizing genetic engineering technique improvement Agronomic Characters in Wheat and cultivating the High-yield Wheat kind and play very vital role to yield traits.Method provided by the invention can be applied to breeding of wheat, realizes early screening, saves time and resource.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is ordinary method.Used test materials among the following embodiment if no special instructions, is to buy from routine biochemistry reagent shop and obtains.% among the following embodiment if no special instructions, is the quality percentage composition.Quantitative test in following examples all is provided with repeated experiments three times, results averaged.The primer is synthetic by Shanghai bio-engineering corporation among the embodiment.
Wheat lines is from the common wheat kind of 169 parts of representational farmers' wheat breeds of Yellow River-Huai River region and local implant mass and Gao Dai strain, (ZM is numbered national germplasm resource bank numbering) specific as follows:
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72, the Henan wheat is No. 10: ZM022975.
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74, Lankao short morning eight: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
75, the Henan wheat is No. 9: ZM017971.
76, lay down the exhibition 4110: Agricultural University Of He'nan; Reference: Shao Yuhua. the influence of formation of 4110 output and output is opened up in date of seeding, density, fertilising to laying down. Anhui agronomy circular, 2008,14:74-75.
77, Henan wheat 14 is excellent: ZM022976.
78, all wheats 18: Agricultural University Of He'nan; Reference: Zheng Jizhou, Yin Guihong, Han Yulin, Yu Haifei, Huang Feng, Lina WANG, Zheng Tiancun. state examines No. 18 Super-high-yielding Cultivation technology of all wheats, Henan agricultural sciences, 2007,7:21-22.
79, the west farming 979: Agricultural University Of He'nan; Reference: Li Xuejun, Li Liqun, Zhang Ruixuan, Wu Yuecheng, Liu is suitable, Wang Hui.The characteristics of agricultural 979 leaf areas in high yield and high quality new variety of wheat west, chlorophyll content and dry-matter accumulation.The wheat crops journal, 2008,28:1054-1057.
80, the Henan wheat 52: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
81, the Henan wheat is No. 3: ZM017967.
82, the Ji wheat 30: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
83, the Henan wheat is No. 1: ZM017965.
84, the Huaihe River wheat 19: Agricultural University Of He'nan; Reference: Liu Youhua, Sun Suyang, Ji Fenggao, Gu Zhengzhong, the Zhang Yun peak, the summer China.The how anti-new variety of wheat of high yield and high quality Huaihe River wheat 19. crop magazines, 2002,1:26.
85, the Henan wheat 21: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
86, numerous 6: Agricultural University Of He'nan; Reference: Niu Yongchun, Wu Liren.Numerous 6-Mianyang is wheat stripe rust resisting variation and countermeasure.Plant Pathology, 1997,27:5-8.
87, the Henan wheat 53: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
88, the Ji 5385: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
89, new wheat 18: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
90, new wheat 3306 (new wheat 16): Agricultural University Of He'nan; Reference: Dong Yun, Zhao Zongwu, horse Warburg Pincus, Liu Chaohui, model Yongsheng, Jiang Zhikai.New No. 16 wheat breed characteristics of wheat and selection are inquired into.The Henan agricultural sciences, 2005,9:24-26.
91, inland river 31:ZM016715.
92, interior township 184: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
93, the Henan wheat 66: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
94, Lankao short morning 8: Agricultural University Of He'nan; Reference: Yang Wenping, Guo Tiancai, Liu Shengbo, Wang Chenyang, Wang Yonghua, Ma Dongyun.The line-spacing configuration is to ' the influence of canopy structure of short eight ' wheat later stage of morning of Lankao and microenvironment thereof.Acta Phytoecologica Sinica, 2008,32:485-490.
95, cylinder: ZM016212.
96, open wheat 18: Agricultural University Of He'nan; Reference: Zhou Yuqin, Niu Benyong, State of Zhao builds, Zhu Yicheng, Shen Yuepeng.Open wheat 18 yielding abilities, yield stability, adaptability, resistance and attributional analysis.The Shaanxi agricultural sciences, 2007,3:7-9.
97, warm wheat 19: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
98, black precious: Agricultural University Of He'nan; Reference: Sun Haitao.The triticale new variety are introduced a fine variety observation bulletin, agriculture of gansu science and technology in the mind black precious No. 1.2007，7：26-27。
99, the Shan farming 757: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
100, the Zheng Yu wheat 9987: Agricultural University Of He'nan; Reference: Li Kuiliang, Han Kunlun, Guo Chunyan.Zheng Yu wheat 9987 features and high-yield culture technique.The China seed industry, 2008,2:76.
101, educate 12 in: Agricultural University Of He'nan; Reference: Yang Zhaosheng, Yan Suhong, Liu Binghua, Liu Hongwei, Li Wei, Yang Li, Zhouyang.Educate 12 in the disease-resistant stable yields new variety of wheat of super high-yielding.World agriculture, 2008,12.
102, the Henan farming 035: Agricultural University Of He'nan; Reference: Li Qiusheng. high yield semi-winterness state examines new variety of wheat Henan farming 035 and cultivation thereof.Plant the industry guide, 2008,7:21-21.
103, the Henan farming 202: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
104, all wheats 23: Agricultural University Of He'nan; Reference: Yang Fang. state examines No. 23 features of all wheats and cultivation technique.2009，9：18。
105, all wheats 20: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
106, the Henan farming 201: Agricultural University Of He'nan; Reference: Dong Zhongdong, Zhan Kehui, Cheng Xiyong, Xu Haixia, Cui Dangqun.The seed selection of high quality noodles new variety of wheat Henan farming 201.The China seed industry, 2008,7:56-57.
107, Zheng wheat 9694: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
108, No. 9, new gram drought: Agricultural University Of He'nan; Reference: Zhang Yanbin, Xin Wenli, Sun Lianfa, Zhang Chunli, Zhao Haibin, Song Qingjie, Xiao Zhimin, Qi Shiyu.SDS-PAGE and A-PAGE Study on difference between No. 9, gram drought and No. 9 wheat breeds of new gram drought.The Heilungkiang agricultural sciences, 2003,1:5～7.
109, new wheat 20 (new wheat 9817): Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
110, the Henan farming 035: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
111, BN 160: Agricultural University Of He'nan; Reference: Chen Qi, Guo Songjing, Zhuo Xiniu.Kind promoted mainly by wheat and the excellent strain comprehensive disease resistance is identified and evaluation.The Henan agricultural sciences, 2007,4:56-58.
112, all wheats 22: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
113, new wheat 208: Agricultural University Of He'nan; Reference: bright red color, Zhou Qinzhi, Zhang Changshun, Chen Meiying, model Yongsheng.Seed selection of the new wheat 208 of new variety of wheat and cultivation technique main points.The agricultural science and technology communication, 2007,11:89-90.
114, the Henan farming 9901: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
115, close No. 6 in Shan: Agricultural University Of He'nan; Reference: Shen Yufang, Qu Dong, Wang Baoli.The arid low-phosphorus stress is to the influence of different varieties wheat root hydraulic conductivity.The northwest Botany Gazette, 2004,24:1578-1582.
116, locust wheat: ZM003734.
117, North China 187: Agricultural University Of He'nan; Reference: Han Jun, Zhang Liansong, Li Jing is graceful, Shi Lijuan, Xie Chaojie, You Mingshan, Yang Zuomin, Liu Guangtian, Sun Qixin, Liu Zhiyong.The heredity of wheat backbone parent " triumph wheat/swallow is big by 1817 " cross combination offspring derived varieties constitutes resolves.Acta Agronomica Sinica, 2009,35:1395-1404.
118, the Henan wheat is No. 8: ZM016051.
119, drought is selected No. 3: ZM009367.
120, No. 3, high yield: Agricultural University Of He'nan; Reference: Gan Aiwa, Lin Ruiming, Xu Shichang, Wan An people, Ma Zhanhong.The China wheat stripe rust differential host No. 3 anti-bars rust sex-controlled inheritances analysis of getting bumper crops.The plant protection journal, 2006,33:369-373.
121, rich anti-13:ZM013111.
122, west wind: MY014236.
123, the Ji wheat 41: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
124, the Henan wheat 55: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
125, duckbill wheat: Agricultural University Of He'nan; Reference: Xu Likui, Zhang Zongchen.The seed selection achievement of the anti-duckbill wheat new variety of temperature wheat series.1997，17：6-8。
126, jasper wheat: ZM017589.
127, Shanxi wheat 33:ZM010374.
128, green ant is No. 4: ZM009594.
129, far away rich 898: Agricultural University Of He'nan; Reference: Zhang Hong, Ren Zhilong.Initial analysis between new strain of wheat rich 898 several proterties far away.The Shaanxi agricultural sciences, 2000,1:3-5.
130, very heavy morning: Agricultural University Of He'nan; Reference: Li Jun, Zhao Huiyan, Li Zhigang, Han Shi farmland, city, Anxin.Wheat Cultivars is to the resistance of grain aphid.Insect knowledge, 2007,44:509-512.
131, the Ji wheat 36: Agricultural University Of He'nan: reference: open blue or green the love. and how we promote No. 36 excellent kinds of wheat of Ji wheat.The Hebei agricultural science and technology, 1997,2:17.
132, No. 1, Jinsha jiang River: Agricultural University Of He'nan; Reference: Wang Guanlin, Bu Xiuling.Dry land wheat (No. one, Jinsha jiang River) * days orchids wheatgrass hybrid rataria callus induction and plant regeneration.The northwest Botany Gazette, 1990,10:84-89.
133, high excellent 503: Agricultural University Of He'nan; Reference: Wang Yanmei. the anti-bar rust of wheat breed " excellent 503 in height " gene chromosomal localization.Acta Agronomica Sinica, 2001,27:383-386.
134, the Ji 6203: Agricultural University Of He'nan; Reference: Lu Xiangcai, Gao Zengyu.The New Winter Wheat Variety brief introduction.The Hebei agricultural science and technology, 2001,6:20.
135, fringe 30:ZM013282 early.
136, lay down for a short time No. 4: Agricultural University Of He'nan: reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
137, the Huaihe River wheat 20: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
138, Lu Nong 55055:ZM015789.
139, the Anhui wheat 38: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
140, new wheat 19: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
141, the Ji is short No. 1: Agricultural University Of He'nan; Reference: Zhang Yongli, Xiao Kai, Li Yanming.Frequency of irrigation is to the influence of short 1/C6-38 Photosynthesis Characteristics of Flag Leaf in hybrid wheat Ji and output.Acta Agronomica Sinica, 2006,32:410-414.
142, the wormwood artemisia wheat 9409: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
143, the Ji wheat 31: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
144, Jimai 20: Agricultural University Of He'nan; Reference: Wang Dong, Yu Zhenwen.Amount of nitrogen is to the influence of the 20 nitrogen sulphur accumulation of strong gluten wheat kind Jimai and reallocation and grain quality.Acta Agronomica Sinica, 2007,33:1439-1445.
145, the Henan farming 949: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
146, the Ji 5265: Agricultural University Of He'nan; Reference: Li Zhibo, Wang Ruihui opens tea, Liang Hong, Ma Zhiying, Zhao Yuxin, Wang Jinghua.Hebei province's wheat breed is based on the analysis of genetic diversity of economical character.The plant genetic resources journal, 2009,10:436-442.
147, Laizhou 953:ZM022727.
148, Zheng spends 57: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
149, the Ji is short No. 9: Agricultural University Of He'nan; Reference: open one, Ni Zhongfu, Yao Yingyin, Sun Qixin.Internode analysis of gene differential expression under the fringe between wheat hybrid and the parent.Acta Agronomica Sinica, 2008,34:770-776.
150, Xichang 76-4:ZM016739.
151, stone 84-7111:ZM017674.
152, tea forms sediment red: Agricultural University Of He'nan; Reference: the father-in-law makes a leapleap forward.The form sediment RFLP molecule marker of red wheat resistant gene of salt of tea.The Hebei agricultural sciences, 1999,3:1-5.
153, No. 1, Denmark: Agricultural University Of He'nan; Reference: Gan Aiwa, Lin Ruiming, Xu Shichang, Wan An people, Ma Zhanhong.The China wheat stripe rust differential host No. 3 anti-bars rust sex-controlled inheritances analysis of getting bumper crops.The plant protection journal, 2006,33:369-373.
154, raise wheat 12: state examines wheat 2001003.
155, Lankao 906: Agricultural University Of He'nan; Reference: Zhu converges, Guo Ruli, Guo Tiancai, Wang Zhijie, Wang Yonghua, Zhang Qingyou.Line-spacing configuration and the influence of density to Lankao 906 colony quality and output.The wheat crops journal, 2001,2:62-66.
156, new wheat 208: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
157, thumb is short: Agricultural University Of He'nan; Reference: ten thousand is flat, Liu Dajun.The ear germinating resistance research that the wheat thumb is short.Agricultural University Of Anhui's journal, 2001,28:15-17.
158, the Henan farming 9676: Agricultural University Of He'nan; Reference: Zhang Qinghai, model Lian, Cui Dangqun, Niu Yunsheng.The Yellow River and Huai He River south sheet wheat is mainly authorized and is promoted wheat breed and seed selection (2008 revised edition) thereof.Scientia Agricultura Sinica press, 2008, Pp:1-394.
159, the Ji wheat 25: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
160, the Henan wheat 16: Agricultural University Of He'nan; Reference: Zhang Qinghai, Wang Zhihe, Zhang Guilan. the Yellow River and Huai He River south sheet wheat application commercial variety and seed selection thereof. Chinese agriculture science and technology press, 2000:1-401.
161, the Henan wheat is No. 4: ZM017968.
162, the Ji 5579: Agricultural University Of He'nan; Reference: open tea, Liang Hong, Wang Ruihui, An Haojun, Wang Jinghua, Zhao Jinfeng, Zhao Yuxin, Chang Wensuo.The main wheat breed prolamine analysis of genetic diversity of planting in Hebei province.In return the agronomy circular, 2008,24:191-196.
163, Zhengzhou 24: Agricultural University Of He'nan; Reference: ZM009466.
164, Fu Zhuang 30: Agricultural University Of He'nan; Reference: Wang Lixin. wheat molecule and cytogenetics Conference Papers summary (22-28 day in April, 2000)---a RAPD mark chain in the wheat breed Fu Zhuang 30 with mildew-resistance gene.Heredity, 2001,23:2001,23:60.
165, bare headed wheat: Agricultural University Of He'nan; Reference: the imperial tinkling of pieces of jade, Liu Hongmei, Feng Mingyi, Lu Shaoyan, Mo Chunbi, Jiang Fuwen, Yue Chaoneng.Wheat Cultivars is to the evaluation of resistance of stripe rust.The Guizhou agricultural sciences, 2009,37:92-95.
The mensuration of embodiment 1, different varieties wheat proterties
Each kind is selected 10 strain materials at random for use, and the result is the mean value of 10 strains.
One, yield traits is measured
Before the seed results, investigate following index: the flag leaf length of every strain and flag leaf width; Boot leaf area=flag leaf length * maximum width of blade * 0.75.After the results, investigation number of grain per ear and every fringe grain are heavy.Measurement result sees Table 1.
Two, seed thousand seed weight and particle size determination
Measure wheat samples thousand seed weight and particle diameter.Measurement result sees Table 1.
The dependency of embodiment 2, wheat Pinb-2v2 gene and proterties
One, identifies puroindoline b-2 gene type with round pcr
1, extracts the wheat grain genomic dna
Extract the wheat grain genomic dna, concrete grammar is: put into the 1.5mL centrifuge tube after 1) smashing with hammer, adding 1mL sample extracting solution (contain 288mM NaCl, 200mM Tris-HCl, 25mM EDTA 0.5%SDS), shook 30 minutes, made its abundant mixing; 2) at 4 ℃, 12, under the 000rpm centrifugal 15 minutes, move supernatant to another 2mL centrifuge tube, added isopyknic phenol/chloroform (1: 1; Volume ratio); 3) 12, centrifugal 15 minutes of 000rpm moves supernatant to another 2mL centrifuge tube, the chloroform/primary isoamyl alcohol of 0.5 times of supernatant volume of adding (24: 1; Volume ratio), fully shake up; 4) 12, centrifugal 10 minutes of 000rpm moves supernatant to another new 2mL centrifuge tube, adds the 3M NaAC aqueous solution (pH=5.2) and the isopropanol precipitating DNA of 0.6 times of supernatant volume, the mixing gently of 1/10 volume; 5) 12, centrifugal 15 minutes of 000rpm abandons supernatant, adds 0.5mL 70% (volumn concentration) aqueous ethanolic solution, leaves standstill 5min; 6) 12,000 centrifugal 5min abandon supernatant.Add 100 μ L TE dissolution precipitations after the vacuum-drying, precipitation is the wheat grain genomic dna.At last, with the concentration of UV spectrophotometer measuring wheat grain genomic dna, preserve standby down for-20 ℃.
2, PCR identifies
1) Puroindoline genotype identification
Because Puroindoline b-2 gene and puroindoline gene nucleotide series similarity are up to about 70%, therefore for guaranteeing that this experiment is not subjected to the puroindoline effect gene, the present invention identifies the puroindoline genotype.The puroindoline genotype of finding in the wheat is divided into three kinds, Pina-D1a/Pinb-D1a, Pina-D1a/Pinb-D1b and Pina-D1a/Pinb-D1p.
With the wheat grain genomic dna is template, identifies that the special primer of Pinb-D1b mutant is:
Primer 1 (upstream): 5 '-ATGAAGACCTTATTCCTCCTA-3 ';
Primer 2 (downstream): 5 '-CTCATGCTCACAGCCGCT-3 '.
With the wheat grain genomic dna is template, adopts Pinb full length gene primer to increase all the other hard type wheats, amplified production order-checking, 12 parts of total Pinb-D1p materials.Pinb full length gene primer is as follows:
Primer 5 (upstream): 5 '-GAGCCTCAACCCATCTATTCATC-3 ';
Primer 6 (downstream): 5 '-CAAGGGTGATTTTATTCATAG-3 '.
2) Puroindoline b-2 genotype identification
Identify that the Pinb-2v2 the primer is as follows:
Primer 3 (upstream): 5 '-CTTGTAGTGAGCACAACCTTTGCA-3 ';
Primer 4 (downstream): 5 '-GTATGGACGAACTTGCAGCTGGAG-3 '.
With the wheat grain genomic dna is template, respectively all wheat breeds is carried out the puroindolineb-2 genotype identification with above-mentioned primer.
Step 1) and step 2) in: the composition of PCR reaction system is: 1.5mmol/L MgCl
2, 0.3mmol/L dNTP, each 10pmol of upstream and downstream primer, template DNA 200ng, 0.5U Taq enzyme adds 1 * PCR damping fluid (containing 10mmol/LTris-HCl, pH9.0,50mmol/L KCl, 1.0%Triton X-100), is settled to 25 μ L.The PCR response procedures is: 94 ℃ of pre-sex change 5min of elder generation; 94 ℃ of sex change 1min then, 58 ℃ of annealing 45sec, 72 ℃ are extended 1min, totally 35 circulations; At last, 72 ℃ are extended 5min.Pcr amplification is got pcr amplification product 10 μ L after finishing, and carries out 1.5% agarose gel electrophoresis and (adopts 1 * TAE damping fluid; Ethidium bromide (EB) dyeing, 160V, 1.5 hours), electrophoresis finishes the back and analyzes with gel imaging system (MultiGenius Gel Documentation and Analysis System) scanning imagery and with computer.Detected result sees Table 1.
The measurement result and the genotype detection result of table 1 different varieties wheat proterties
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??1 | ??36.1 | ??2 | ??39 | ??1.38 | ??1.06 | ??25 | ??19.9 | Not |
| ??2 | ??34.1 | ??2.1 | ??33 | ??1.1 | ??1.28 | ??21 | ??20.2 | Not |
| ??3 | ??35.9 | ??2.2 | ??43 | ??1.59 | ??1.33 | ??21 | ??20.9 | Not |
| ??4 | ??27.1 | ??1.8 | ??43 | ??1.08 | ??1.37 | ??22 | ??22.6 | Not |
| ??5 | ??49.9 | ??2.8 | ??39 | ??1.9 | ??1.42 | ??17 | ??18.1 | Not |
| ??6 | ??29.2 | ??1.8 | ??40 | ??0.96 | ??1.43 | ??34 | ??36.5 | Not |
| ??7 | ??50.2 | ??3.1 | ??45 | ??2.04 | ??1.44 | ??18 | ??19.4 | Not |
| ??8 | ??36.6 | ??2.1 | ??42 | ??1.22 | ??1.45 | ??17 | ??18.5 | Not |
| ??9 | ??31.9 | ??2 | ??52 | ??1.78 | ??1.45 | ??21 | ??22.8 | Not |
| ??10 | ??42.9 | ??2.5 | ??37 | ??1.63 | ??1.49 | ??14 | ??15.6 | Not |
| ??11 | ??34.2 | ??2.2 | ??30 | ??1.08 | ??1.51 | ??16 | ??18.1 | Not |
| ??12 | ??51.9 | ??3.1 | ??41 | ??1.95 | ??1.53 | ??18 | ??20.7 | Not |
| ??13 | ??29.5 | ??1.7 | ??66 | ??1.89 | ??1.54 | ??27 | ??31.2 | Not |
| ??14 | ??26.7 | ??1.8 | ??36 | ??0.86 | ??1.56 | ??27 | ??31.6 | Not |
| ??15 | ??37.4 | ??2 | ??51 | ??1.49 | ??1.57 | ??20 | ??23.6 | Not |
| ??16 | ??37.7 | ??2.1 | ??46 | ??1.77 | ??1.57 | ??24 | ??28.3 | Not |
| ??17 | ??47.8 | ??2.4 | ??49 | ??2.31 | ??1.61 | ??17 | ??20.5 | Not |
| ??18 | ??48.1 | ??3.1 | ??50 | ??1.69 | ??1.61 | ??18 | ??21.7 | Not |
| ??19 | ??50.4 | ??2.8 | ??39 | ??1.8 | ??1.61 | ??22 | ??26.6 | Not |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??20 | ??45.5 | ??2.8 | ??46 | ??1.85 | ??1.62 | ??19 | ??23.1 | Not |
| ??21 | ??45.3 | ??2.7 | ??46 | ??2.02 | ??1.64 | ??20 | ??24.6 | Not |
| ??22 | ??47 | ??2.7 | ??57 | ??2.27 | ??1.67 | ??21 | ??26.3 | Not |
| ??23 | ??44.6 | ??2.6 | ??57 | ??2.68 | ??1.67 | ??22 | ??27.6 | Not |
| ??24 | ??49.3 | ??2.8 | ??41 | ??2.01 | ??1.7 | ??21 | ??26.8 | Not |
| ??25 | ??42 | ??2.7 | ??42 | ??1.83 | ??1.71 | ??17 | ??21.8 | Not |
| ??26 | ??44.2 | ??2.3 | ??49 | ??2.12 | ??1.72 | ??17 | ??21.9 | Not |
| ??27 | ??50.6 | ??2.7 | ??49 | ??2.81 | ??1.73 | ??17 | ??22.1 | Not |
| ??28 | ??30.4 | ??1.9 | ??37 | ??1.24 | ??1.73 | ??27 | ??35.0 | Not |
| ??29 | ??38.4 | ??2.3 | ??53 | ??2.64 | ??1.74 | ??20 | ??26.1 | Not |
| ??30 | ??40.8 | ??2.4 | ??44 | ??1.73 | ??1.75 | ??15 | ??19.7 | Not |
| ??31 | ??56.9 | ??3 | ??50 | ??2.61 | ??1.75 | ??20 | ??26.3 | Not |
| ??32 | ??42 | ??2.3 | ??28 | ??1.23 | ??1.76 | ??19 | ??25.1 | Not |
| ??33 | ??47.6 | ??2.6 | ??46 | ??2.5 | ??1.76 | ??22 | ??29.0 | Not |
| ??34 | ??50.6 | ??3 | ??36 | ??1.84 | ??1.76 | ??23 | ??30.4 | Not |
| ??35 | ??59.7 | ??3.4 | ??44 | ??2.27 | ??1.77 | ??17 | ??22.6 | Not |
| ??36 | ??49.6 | ??2.5 | ??50 | ??2.28 | ??1.78 | ??15 | ??20.0 | Not |
| ??37 | ??45.9 | ??2.6 | ??50 | ??2.07 | ??1.78 | ??16 | ??21.4 | Not |
| ??38 | ??42.9 | ??2.4 | ??54 | ??2.21 | ??1.78 | ??18 | ??24.0 | Not |
| ??39 | ??41.9 | ??2.5 | ??46 | ??1.88 | ??1.78 | ??18 | ??24.0 | Not |
| ??40 | ??41.9 | ??2.4 | ??50 | ??2.14 | ??1.79 | ??19 | ??25.5 | Not |
| ??41 | ??39 | ??2.3 | ??51 | ??1.63 | ??1.79 | ??23 | ??30.9 | Not |
| ??42 | ??50.4 | ??3 | ??50 | ??2.43 | ??1.8 | ??18 | ??24.3 | Not |
| ??43 | ??50.6 | ??3 | ??42 | ??2.09 | ??1.81 | ??18 | ??24.4 | Not |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??44 | ??49.7 | ??2.5 | ??52 | ??2.36 | ??1.81 | ??20 | ??27.2 | Not |
| ??45 | ??40.9 | ??2.3 | ??44 | ??2.13 | ??1.82 | ??17 | ??23.2 | Not |
| ??46 | ??45.5 | ??2.6 | ??45 | ??1.84 | ??1.83 | ??17 | ??23.3 | Not |
| ??47 | ??49.2 | ??2.7 | ??55 | ??2.35 | ??1.84 | ??18 | ??24.8 | Not |
| ??48 | ??42 | ??2.5 | ??54 | ??2.44 | ??1.84 | ??19 | ??26.2 | Not |
| ??49 | ??47.6 | ??2.7 | ??77 | ??3.78 | ??1.84 | ??21 | ??29.0 | Not |
| ??50 | ??46.6 | ??2.6 | ??45 | ??2.1 | ??1.85 | ??20 | ??27.8 | Not |
| ??51 | ??40.2 | ??2.2 | ??53 | ??1.86 | ??1.86 | ??19 | ??26.5 | Not |
| ??52 | ??43.7 | ??2.5 | ??42 | ??1.72 | ??1.87 | ??17 | ??23.8 | Not |
| ??53 | ??33.7 | ??1.9 | ??57 | ??2.27 | ??1.87 | ??21 | ??29.5 | Not |
| ??54 | ??53.1 | ??3.1 | ??49 | ??2.39 | ??1.88 | ??24 | ??33.8 | Not |
| ??55 | ??39.1 | ??2.1 | ??55 | ??2.56 | ??1.88 | ??25 | ??35.3 | Not |
| ??56 | ??37.9 | ??2.1 | ??42 | ??1.49 | ??1.89 | ??16 | ??22.7 | Not |
| ??57 | ??60.8 | ??3.4 | ??44 | ??2.52 | ??1.9 | ??16 | ??22.8 | Not |
| ??58 | ??56.9 | ??3.1 | ??37 | ??2.01 | ??1.9 | ??19 | ??27.1 | Not |
| ??59 | ??54.1 | ??3 | ??57 | ??2.83 | ??1.9 | ??20 | ??28.5 | Not |
| ??60 | ??58.5 | ??3.2 | ??52 | ??3.06 | ??1.9 | ??20 | ??28.5 | Not |
| ??61 | ??52.6 | ??3.1 | ??44 | ??2.63 | ??1.92 | ??24 | ??34.6 | Not |
| ??62 | ??58.9 | ??3.2 | ??44 | ??2.16 | ??1.92 | ??25 | ??36.0 | Not |
| ??63 | ??48.2 | ??2.7 | ??59 | ??2.71 | ??1.93 | ??19 | ??27.5 | Not |
| ??64 | ??42.5 | ??2.7 | ??35 | ??1.65 | ??1.93 | ??21 | ??30.4 | Not |
| ??65 | ??45.7 | ??2.8 | ??52 | ??2.33 | ??1.95 | ??18 | ??26.3 | Not |
| ??66 | ??46.7 | ??2.8 | ??39 | ??2.12 | ??1.95 | ??21 | ??30.7 | Not |
| ??67 | ??54.7 | ??3.2 | ??51 | ??2.74 | ??1.95 | ??24 | ??35.1 | Not |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??68 | ??46.9 | ??2.8 | ??68 | ??3.14 | ??1.96 | ??19 | ??27.9 | Not |
| ??69 | ??49.9 | ??2.5 | ??52 | ??2.85 | ??1.97 | ??22 | ??32.5 | Not |
| ??70 | ??48.7 | ??2.8 | ??64 | ??2.4 | ??1.98 | ??20 | ??29.7 | Not |
| ??71 | ??48.9 | ??2.4 | ??39 | ??1.85 | ??1.98 | ??21 | ??31.2 | Not |
| ??72 | ??53.1 | ??3 | ??49 | ??2.76 | ??1.98 | ??21 | ??31.2 | Not |
| ??73 | ??51 | ??2.9 | ??54 | ??3.37 | ??1.98 | ??22 | ??32.7 | Not |
| ??74 | ??24.1 | ??1.7 | ??63 | ??1.81 | ??1.98 | ??22 | ??32.7 | Not |
| ??75 | ??38.5 | ??2 | ??64 | ??2.66 | ??1.99 | ??26 | ??38.8 | Not |
| ??76 | ??57.4 | ??3.1 | ??45 | ??2.59 | ??2 | ??19 | ??28.5 | Not |
| ??77 | ??31.7 | ??2.1 | ??63 | ??2.39 | ??2 | ??19 | ??28.5 | Not |
| ??78 | ??49.7 | ??2.9 | ??69 | ??2.94 | ??2 | ??20 | ??30.0 | Not |
| ??79 | ??51.7 | ??2.9 | ??56 | ??2.54 | ??2 | ??21 | ??31.5 | Not |
| ??80 | ??45.2 | ??2.7 | ??53 | ??2.56 | ??2.01 | ??20 | ??30.2 | Not |
| ??81 | ??36.9 | ??2 | ??58 | ??2.07 | ??2.01 | ??24 | ??36.2 | Not |
| ??82 | ??49.2 | ??2.5 | ??37 | ??2.02 | ??2.04 | ??23 | ??35.2 | Not |
| ??83 | ??43.7 | ??2.7 | ??53 | ??2.34 | ??2.04 | ??28 | ??42.8 | Not |
| ??84 | ??54.5 | ??3.1 | ??44 | ??2.39 | ??2.05 | ??17 | ??26.1 | Not |
| ??85 | ??46.2 | ??2.5 | ??59 | ??2.75 | ??2.05 | ??21 | ??32.3 | Not |
| ??86 | ??33.3 | ??1.9 | ??48 | ??1.89 | ??2.06 | ??21 | ??32.4 | Not |
| ??87 | ??51 | ??2.9 | ??56 | ??3.27 | ??2.09 | ??23 | ??36.1 | Not |
| ??88 | ??49.1 | ??2.7 | ??46 | ??2.39 | ??2.1 | ??20 | ??31.5 | Not |
| ??89 | ??39.7 | ??2.5 | ??44 | ??1.87 | ??2.1 | ??21 | ??33.1 | Not |
| ??90 | ??41.3 | ??2.4 | ??58 | ??2.48 | ??2.1 | ??21 | ??33.1 | Not |
| ??91 | ??37.4 | ??2.3 | ??59 | ??1.56 | ??2.13 | ??23 | ??36.7 | Not |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??92 | ??53.5 | ??2.9 | ??61 | ??3.17 | ??2.17 | ??26 | ??42.3 | Not |
| ??93 | ??28.7 | ??1.9 | ??42 | ??1.14 | ??2.18 | ??21 | ??34.3 | Not |
| ??94 | ??49.8 | ??3 | ??54 | ??2.81 | ??2.2 | ??20 | ??33.0 | Not |
| ??95 | ??23.8 | ??1.5 | ??83 | ??2.34 | ??2.23 | ??27 | ??45.2 | Not |
| ??96 | ??51.2 | ??2.7 | ??61 | ??2.64 | ??2.24 | ??21 | ??35.3 | Not |
| ??97 | ??47.4 | ??2.7 | ??58 | ??3.06 | ??2.26 | ??24 | ??40.7 | Not |
| ??98 | ??31.6 | ??2.1 | ??76 | ??2.31 | ??2.36 | ??24 | ??42.5 | Not |
| ??99 | ??37.2 | ??2 | ??100 | ??2.81 | ??2.79 | ??27 | ??56.5 | Not |
| ??100 | ??49.4 | ??2.8 | ??50 | ??2.07 | ??1.78 | ??16 | ??21.4 | Not |
| ??101 | ??48.3 | ??2.9 | ??56 | ??3.37 | ??2.09 | ??23 | ??36.1 | Not |
| ??102 | ??54.9 | ??3.2 | ??53 | ??3.09 | ??2.1 | ??25 | ??39.4 | Not |
| ??103 | ??49 | ??3 | ??43 | ??1.08 | ??1.37 | ??22 | ??22.6 | Not |
| ??104 | ??50.5 | ??3.2 | ??56 | ??3.27 | ??2.09 | ??23 | ??36.1 | Not |
| ??105 | ??50.3 | ??3 | ??49 | ??2.31 | ??1.61 | ??17 | ??20.5 | Not |
| ??106 | ??44.6 | ??2.6 | ??51 | ??2.61 | ??1.75 | ??20 | ??26.3 | Not |
| ??107 | ??53.2 | ??2.9 | ??45 | ??2.04 | ??1.44 | ??18 | ??19.4 | Not |
| ??108 | ??41.1 | ??2.4 | ??47 | ??1.88 | ??1.62 | ??19 | ??23.1 | Not |
| ??109 | ??48 | ??2.8 | ??45 | ??2.14 | ??1.44 | ??18 | ??19.4 | Not |
| ??110 | ??56.4 | ??2.9 | ??48 | ??1.57 | ??1.61 | ??22 | ??26.6 | Not |
| ??111 | ??37.6 | ??2.2 | ??58.6 | ??1.43 | ??1.26 | ??20 | ??18.9 | Not |
| ??112 | ??56.5 | ??3.1 | ??59 | ??2.65 | ??2.19 | ??20 | ??32.9 | Not |
| ??113 | ??45.6 | ??2.5 | ??42 | ??1.95 | ??1.53 | ??18 | ??20.7 | Not |
| ??114 | ??61.4 | ??3.6 | ??52 | ??1.78 | ??1.45 | ??21 | ??22.8 | Not |
| ??115 | ??40.6 | ??2.5 | ??28 | ??1.03 | ??1.25 | ??16 | ??15.0 | Be |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??116 | ??28 | ??1.7 | ??52 | ??1.12 | ??1.32 | ??22 | ??21.8 | Be |
| ??117 | ??43.5 | ??2.6 | ??36 | ??1.57 | ??1.36 | ??17 | ??17.3 | Be |
| ??118 | ??45.5 | ??2.7 | ??41 | ??1.66 | ??1.37 | ??15 | ??15.4 | Be |
| ??119 | ??37.6 | ??2.3 | ??49 | ??2.03 | ??1.39 | ??20 | ??20.9 | Be |
| ??120 | ??38.4 | ??2.2 | ??35 | ??1.2 | ??1.4 | ??19 | ??20.0 | Be |
| ??121 | ??41.6 | ??2.7 | ??37 | ??1.47 | ??1.42 | ??15 | ??16.0 | Be |
| ??122 | ??33.4 | ??1.9 | ??43 | ??1.69 | ??1.49 | ??21 | ??23.5 | Be |
| ??123 | ??44.6 | ??2.7 | ??49 | ??1.5 | ??1.53 | ??23 | ??26.4 | Be |
| ??124 | ??39.1 | ??2.5 | ??35 | ??1.26 | ??1.55 | ??19 | ??22.1 | Be |
| ??125 | ??34.1 | ??1.9 | ??52 | ??1.64 | ??1.58 | ??20 | ??23.7 | Be |
| ??126 | ??32.4 | ??1.9 | ??28 | ??1.22 | ??1.59 | ??21 | ??25.0 | Be |
| ??127 | ??39.8 | ??2 | ??51 | ??2.16 | ??1.59 | ??21 | ??25.0 | Be |
| ??128 | ??31.4 | ??2 | ??49 | ??1.53 | ??1.61 | ??21 | ??25.4 | Be |
| ??129 | ??35.7 | ??2.1 | ??39 | ??1.16 | ??1.66 | ??19 | ??23.7 | Be |
| ??130 | ??35.2 | ??2.2 | ??31 | ??1.18 | ??1.71 | ??22 | ??28.2 | Be |
| ??131 | ??44.8 | ??2.5 | ??39 | ??1.8 | ??1.72 | ??18 | ??23.2 | Be |
| ??132 | ??35.6 | ??2.1 | ??42 | ??1.41 | ??1.73 | ??23 | ??29.8 | Be |
| ??133 | ??39.2 | ??2.4 | ??50 | ??1.92 | ??1.76 | ??22 | ??29.0 | Be |
| ??134 | ??43.2 | ??2.4 | ??46 | ??1.76 | ??1.77 | ??19 | ??25.2 | Be |
| ??135 | ??43.8 | ??2.4 | ??41 | ??1.83 | ??1.78 | ??15 | ??20.0 | Be |
| ??136 | ??36.6 | ??2.1 | ??50 | ??1.5 | ??1.78 | ??19 | ??25.4 | Be |
| ??137 | ??47.1 | ??2.8 | ??60 | ??2.38 | ??1.8 | ??18 | ??24.3 | Be |
| ??138 | ??38.5 | ??2.3 | ??38 | ??1.88 | ??1.84 | ??23 | ??31.7 | Be |
| ??139 | ??42.8 | ??2.7 | ??53 | ??2.15 | ??1.87 | ??15 | ??21.0 | Be |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??140 | ??39.7 | ??2.3 | ??51 | ??1.85 | ??1.89 | ??20 | ??28.4 | Be |
| ??141 | ??41 | ??2.5 | ??52 | ??2.48 | ??1.92 | ??20 | ??28.8 | Be |
| ??142 | ??37.4 | ??2.3 | ??63 | ??2.58 | ??1.93 | ??18 | ??26.1 | Be |
| ??143 | ??43.3 | ??2.5 | ??59 | ??2.8 | ??1.93 | ??22 | ??31.8 | Be |
| ??144 | ??53 | ??3.1 | ??48 | ??2.28 | ??1.94 | ??19 | ??27.6 | Be |
| ??145 | ??40.5 | ??2.3 | ??45 | ??2.43 | ??1.98 | ??24 | ??35.6 | Be |
| ??146 | ??52.9 | ??2.8 | ??41 | ??2.34 | ??2.07 | ??17 | ??26.4 | Be |
| ??147 | ??53.2 | ??3 | ??54 | ??2.79 | ??2.07 | ??21 | ??32.6 | Be |
| ??148 | ??46.2 | ??2.7 | ??50 | ??2.56 | ??2.08 | ??22 | ??34.3 | Be |
| ??149 | ??49.3 | ??2.9 | ??42 | ??2.06 | ??2.09 | ??19 | ??29.8 | Be |
| ??150 | ??39 | ??2.1 | ??59 | ??2.63 | ??2.14 | ??20 | ??32.1 | Be |
| ??151 | ??42.5 | ??2.6 | ??40.9 | ??1.66 | ??1.69 | ??18 | ??22.8 | Not |
| ??152 | ??24.5 | ??1.5 | ??42.44 | ??1.04 | ??1.18 | ??21 | ??18.6 | Not |
| ??153 | ??21.3 | ??1.4 | ??31.75 | ??0.94 | ??1.76 | ??30 | ??39.6 | Not |
| ??154 | ??49.4 | ??2.6 | ??47.9 | ??2.16 | ??1.92 | ??20 | ??28.8 | Not |
| ??155 | ??25.9 | ??1.6 | ??41.9 | ??1.14 | ??2.18 | ??21 | ??34.3 | Not |
| ??156 | ??27 | ??1.7 | ??39.2 | ??1.02 | ??2.22 | ??19 | ??31.6 | Not |
| ??157 | ??26.7 | ??1.8 | ??- | ??- | ??2.03 | ??16 | ??24.4 | Be |
| ??158 | ??50.9 | ??3.1 | ??45 | ??2.04 | ??1.64 | ??18 | ??22.1 | Not |
| ??159 | ??45.5 | ??2.5 | ??30 | ??1.46 | ??1.73 | ??19 | ??24.7 | Not |
| ??160 | ??40.2 | ??2.3 | ??57.1 | ??1.97 | ??2.15 | ??24 | ??38.7 | Not |
| ??161 | ??37 | ??2.2 | ??62.9 | ??2.22 | ??2.05 | ??22 | ??33.8 | Not |
| ??162 | ??57 | ??3 | ??27.9 | ??1.3 | ??1.69 | ??18 | ??22.8 | Not |
| ??163 | ??30.9 | ??1.9 | ??56 | ??2.7 | ??1.92 | ??22 | ??31.7 | Be |
| Numbering | Thousand seed weight (mg) | Particle diameter (mm) | Grain number per spike (individual) | Every fringe grain heavy (g) | Boot leaf wide (cm) | Boot leaf long (cm) | Boot leaf area (cm 2) | Whether has the PCR product |
| ??164 | ??25.4 | ??1.8 | ??58.7 | ??1.33 | ??1.26 | ??19 | ??18.0 | Be |
| ??165 | ??22.4 | ??1.9 | ??28 | ??1.22 | ??1.59 | ??21 | ??25.0 | Be |
There is the pcr amplification product electrophoresis showed of 40 parts of materials to have band, all PCR products that obtain are checked order, find that the nucleotide sequence of PCR product is all held shown in the 34th to 434 Nucleotide from 5 ' as the sequence 2 of sequence table.With the nucleotide sequence of PCR product as the sequence 2 of sequence table genotype called after Pinb-2v2 genotype from the wheat shown in the 34th to 434 Nucleotide of 5 ' end.
Generally, have that the wheat breed thousand seed weight, particle diameter, grain number per spike, every fringe grain of Pinb-2v2 gene is heavy, boot leaf area and flag leaf width all significantly be lower than the kind that does not contain Pinb-2v2.For eliminating may influencing of puroindoline gene pairs wheat yield proterties, further strengthen data reliability, compare belonging to the genotypic sample of the different puroindoline b-2 of identical puroindoline genotype respectively, the wheat breed thousand seed weight, particle diameter, grain number per spike, every fringe grain weight, boot leaf area and the flag leaf width that have Pinb-2v2 all significantly are lower than the kind (seeing Table 2) that does not contain Pinb-2v2.
Table 2 wheat Pinb-2v2 genotype and other genotype yield traits comparison sheet
Annotate: different alphabetical differences show that difference reaches 5% conspicuous level.
Sequence table
＜110〉Agricultural University Of He'nan
＜120〉assistant identification has the method for wheat of good character
<130>CCGNARY102162
<160>4
<210>1
<211>150
<212>PRT
＜213〉Triticum wheat (Triticum aestivum L.)
<400>1
Met?Lys?Thr?Leu?Phe?Leu?Leu?Ala?Leu?Leu?Ser?Leu?Val?Val?Ser?Thr
1???????????????5???????????????????10??????????????????15
Thr?Phe?Ala?Gln?Tyr?Lys?Glu?Val?Gly?Gly?Ser?Tyr?Glu?Asn?Gly?Gly
20??????????????????25??????????????????30
Arg?Gly?Phe?Gly?Ser?Gln?Asn?Cys?Pro?Pro?Glu?Lys?Thr?Lys?Leu?Asp
35??????????????????40??????????????????45
Ser?Cys?Lys?Asp?Tyr?Val?Met?Glu?Arg?Cys?Leu?Ala?Val?Lys?Gly?Phe
50??????????????????55??????????????????60
Ser?Ile?Ala?Arg?Leu?Leu?Lys?Trp?Trp?Lys?Gly?Ala?Cys?Glu?Gln?Glu
65??????????????????70??????????????????75??????????????????80
Ala?Leu?Asp?Gln?Cys?Cys?Gln?Gln?Leu?Arg?Pro?Val?Val?Lys?Lys?Cys
85??????????????????90??????????????????95
Arg?Cys?Glu?Ala?Ile?Trp?Arg?Ala?Val?Gln?Gly?Asn?Leu?Gly?Gly?Val
100?????????????????105?????????????????110
Phe?Gly?Phe?Gln?Gln?Gly?Lys?Ile?Thr?Lys?Gln?Ile?Gln?Arg?Ala?Met
115?????????????????120?????????????????125
Met?Leu?Pro?Ser?Lys?Cys?Lys?Met?Asp?Ser?Ser?Cys?Lys?Phe?Val?His
130?????????????????135?????????????????140
Thr?Asn?Gly?Tyr?Tyr?Tyr
145?????????????????150
<210>2
<211>453
<212>DNA
＜213〉Triticum wheat (Triticum aestivum L.)
<400>2
atgaagacct?tattcctcct?agctctcctt?tctcttgtag?tgagcacaac?ctttgcacaa?????60
tacaaagagg?ttggcggcag?ctatgagaac?ggtggtagag?ggtttggttc?tcaaaactgc????120
ccaccggaga?agacgaagct?ggactcttgc?aaggattatg?tgatggagcg?gtgcttggcg????180
gtgaagggtt?tttcgatcgc?cagacttttg?aaatggtgga?agggcgcctg?tgaacaagag????240
gccctagacc?aatgttgcca?gcaactacgc?ccggtagtaa?agaagtgccg?atgtgaggcc????300
atttggaggg?ctgtccaagg?caaccttggt?ggcgtctttg?gctttcagca?aggcaagata????360
acgaaacaaa?ttcagagggc?catgatgttg?ccctccaaat?gcaaaatgga?ctccagctgc????420
aagttcgtcc?atactaatgg?ctattactac?tga?????????????????????????????????453
<210>3
<211>24
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>3
cttgtagtga?gcacaacctt?tgca?????????????????????????????????????????24
<210>4
<211>24
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>4
gtatggacga?act?tgcagct?ggag???????????????????????24
Claims (10)
1. an assistant identification has the various trait method of wheat, comprise the steps: that the genomic dna with wheat to be measured is a template, the primer of forming with DNA shown in the sequence 4 of DNA shown in the sequence 3 of sequence table and sequence table carries out following 1 then to carrying out pcr amplification) to 6) in arbitrary described judgement:
1) can amplify the thousand seed weight of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
2) can amplify the particle diameter of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
3) the fringe grain that can amplify the wheat of dna fragmentation focuses on the statistics less than the wheat that can not amplify dna fragmentation;
4) can amplify the grain number per spike of wheat of dna fragmentation statistically less than the wheat that can not amplify dna fragmentation;
5) the boot leaf area of wheat that can amplify dna fragmentation at statistics less than the wheat that can not amplify dna fragmentation;
6) flag leaf width of wheat that can amplify dna fragmentation at statistics less than the wheat that can not amplify dna fragmentation.
2. the method for claim 1 is characterized in that: 1) to 6) in, described dna fragmentation is the dna fragmentation of 401bp.
3. the primer of the composition of DNA shown in the sequence 4 of DNA and sequence table shown in the sequence 3 of sequence table is right.
4. the described primer of claim 3 is to the application in the test kit that has the various trait wheat in the preparation assistant identification; Described proterties is that thousand seed weight, particle diameter, grain number per spike, every fringe grain are heavy, at least a in boot leaf area and the flag leaf width.
5. assistant identification has the test kit of various trait wheat, comprises that the described primer of claim 3 is right; Described proterties is that thousand seed weight, particle diameter, grain number per spike, every fringe grain are heavy, at least a in boot leaf area and the flag leaf width.
6. an assistant identification has the various trait method of wheat, is to detect Nucleotide shown in the sequence 2 that whether has sequence table in the genomic dna of wheat to be measured, carries out following 1 then) to 6) in arbitrary described judgement:
1) thousand seed weight that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
2) particle diameter that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
3) the fringe grain that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna focuses on the statistics wheat less than Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
4) grain number per spike that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna is statistically less than the wheat of Nucleotide shown in the sequence 2 that does not have sequence table in the genomic dna;
5) the boot leaf area that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna does not have the wheat of Nucleotide shown in the sequence 2 of sequence table in less than genomic dna at statistics;
6) flag leaf width that has the wheat of Nucleotide shown in the sequence 2 of sequence table in the genomic dna does not have the wheat of Nucleotide shown in the sequence 2 of sequence table in less than genomic dna at statistics.
7. an assistant identification has the various trait method of wheat, is to detect the sequence 2 that whether has sequence table in the genomic dna of wheat to be measured from Nucleotide shown in the 34th to 434 at the 5 ' end, carries out following 1 then) to 6) in arbitrary described judgement:
1) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the thousand seed weight of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
2) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the particle diameter of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
3) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in the fringe grain of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end focuses on the statistics less than genomic dna is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
4) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in statistically less than genomic dna from the grain number per spike of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
5) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in less than genomic dna at statistics from the boot leaf area of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end;
6) sequence 2 that has sequence table in the genomic dna sequence 2 that do not have sequence table in less than genomic dna at statistics from the flag leaf width of the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end is from the wheat of Nucleotide shown in the 34th to 434 at the 5 ' end.
8. claim 1 or 2 described methods, the described primer of claim 3 is right, the described test kit of claim 5, the described method of claim 6 or the application of the described method of claim 7 in wheat breeding.
9. protein is following (a) or (b):
(a) protein of forming by the aminoacid sequence shown in the sequence in the sequence table 1;
(b) with the aminoacid sequence of sequence 1 through the replacement of one or several amino-acid residue and/or disappearance and/or interpolation and relevant with the Wheat Production proterties by sequence 1 deutero-protein.
10. coding claim 1 described proteic gene is preferably as follows 1) or 2) or 3) or 4) dna molecular:
1) dna molecular shown in the sequence 2 in the sequence table;
2) sequence 2 is held the dna molecular shown in the 34th to 434 Nucleotide from 5 ' in the sequence table;
3) under stringent condition with 1) or 2) the dna sequence dna hybridization that limits and the dna molecular of coding Wheat Production character-related protein;
4) with 1) or 2) or 3) dna sequence dna that limits has 90% above homology and the dna molecular of the Wheat Production character-related protein of encoding.
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Cited By (3)
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| CN103060319A (en) * | 2013-01-17 | 2013-04-24 | 中国农业科学院作物科学研究所 | Primer pair for assaying thousand-grain weight of wheat and related molecular marker |
| CN104774934A (en) * | 2015-04-01 | 2015-07-15 | 周口市农业科学院 | Method for identifying Zhoumai No.23 wheat variety |
| CN111944030A (en) * | 2020-08-25 | 2020-11-17 | 河南科技学院 | Wheat stress resistance regulatory protein TaCOR58 and coding gene and application thereof |
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2010
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| 《Theor Appl Genet》 20091113 Feng Chen Physical mapping and a new variant of Puroindoline b-2 genes in wheat 747页表1、749页图2 , 第120期 2 * |
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| CN103060319A (en) * | 2013-01-17 | 2013-04-24 | 中国农业科学院作物科学研究所 | Primer pair for assaying thousand-grain weight of wheat and related molecular marker |
| CN103060319B (en) * | 2013-01-17 | 2014-04-16 | 中国农业科学院作物科学研究所 | Primer pair for assaying thousand-grain weight of wheat and related molecular marker |
| CN104774934A (en) * | 2015-04-01 | 2015-07-15 | 周口市农业科学院 | Method for identifying Zhoumai No.23 wheat variety |
| CN104774934B (en) * | 2015-04-01 | 2017-07-25 | 周口市农业科学院 | A kind of method for identifying No. 23 wheat breeds of all wheats |
| CN111944030A (en) * | 2020-08-25 | 2020-11-17 | 河南科技学院 | Wheat stress resistance regulatory protein TaCOR58 and coding gene and application thereof |
| CN111944030B (en) * | 2020-08-25 | 2022-03-15 | 河南科技学院 | Wheat stress resistance regulatory protein TaCOR58 and coding gene and application thereof |
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