CN101798562A - Device and method for strengthening solid state fermentation - Google Patents
Device and method for strengthening solid state fermentation Download PDFInfo
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- CN101798562A CN101798562A CN201010113576A CN201010113576A CN101798562A CN 101798562 A CN101798562 A CN 101798562A CN 201010113576 A CN201010113576 A CN 201010113576A CN 201010113576 A CN201010113576 A CN 201010113576A CN 101798562 A CN101798562 A CN 101798562A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
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Abstract
The invention discloses a device and a method for strengthening solid state fermentation, which is mainly characterized in that the bottom and the inner wall of a fermentation tank are provided with air sacs which are periodically agitated; under the condition of producing no shearing force, solid material in the fermentation tank is evenly stirred; meanwhile, an air compressor, an air storage tank and the fermentation tank form closed cycle which can generate periodic gas flow and quicken the mass transfer and the heat transfer of solid material. The device and the method solve the problems of uneven reaction and low fermentation rate of solid state fermentation matters due to difficult mass transfer and heat transfer, can be used for solidly ferment and produce a large quantity of fermentation products, such as methane, ethanol, butanol and the like, shortens fermentation period and improves fermentation strength and raw material conversion rate.
Description
Technical field
The invention belongs to the fermentation field, particularly a kind of apparatus and method of strengthening solid state fermentation.
Technical background
Solid state fermentation has had history in several thousand as a kind of ancient fermentation mode, at B.C. 2600, people adopt the mode of solid state fermentation to produce bread with regard to beginning, be exactly in nearly several centuries, solid state fermentation also has a wide range of applications at the production and the aspects such as ensiling and compost of fermented soya bean, fermented bean curd, sauce vinegar etc., in recent years owing to extensive deep layer liquid state fermentation broad application and immense success, solid state fermentation has almost been ignored fully.In nearest 15～20 years.Because the characteristics of solid state fermentation self and advantage that liquid submerged fermentation did not have and be subject to people's attention again, in the production of production, biogas, ethanol, butanols, organic acid production and the fungal metabolite or the fungal spore of agriculture and industry waste processing, enzyme, more and more used.Solid state fermentation is compared with liquid state fermentation, has a lot of advantages and characteristics: 1) with low cost, and the fermentation substrate wide material sources; 2) some leavened prod only produces when solid state fermentation, perhaps all produces under solid-state and liquid condition, generates product but more help fermentation at solid state fermentation conditions; 3) handle agricultural wastes and domestic waste by solid state fermentation, help protecting environment, reduce the discharging of organic solid, can produce bioenergy and bio-based chemical simultaneously, as ethanol, biogas, Succinic Acid, 2,3-butyleneglycol, fertilizer etc.; 4) there is not " three wastes " discharging; 5) energy consumption is low, less investment.
But solid state fermentation also has its natural defect: solid substances is unfavorable for mass transfer, heat transfer, and fermenting process stirs difficulty and maybe should not stir, and causes that fermenting process is difficult to control, fermentation efficiency is low, the cycle is long, product is unstable.People attempt to develop various solid-state fermentation reactors and come reinforcing mass transfer, conduct heat, obtained certain effect, that have even be applied to industrialization, the gas-phase double-dynamic solid fermentation method and the fermentation unit (patent No.: 02100176.6) thereof have been developed as us, material inserted pressure pulsation and circulate in two dynamic environment of air and carry out solid state fermentation, this gas-phase double attitude technology and device cause triple effect: (1) quickens microorganism metabolism, (2) quicken nutrition and the transmission of meta-bolites inside and outside cell, (3) between solid medium accumulation horizon endoparticle in the space in gas phase and the reactor the intersubjective heat and mass transport mode of gas phase will change the convection current diffusion into by molecular diffusion, this reaction unit is 100 cubic metres technical scale, be applied to biological pesticide and cellulose solid-state fermentation, obtained good economic benefits.But this gas-phase double attitude is a moving phase with filtering sterile air, for anaerobic solid-state fermentation and incompatible.The Tong Jin of Jilin University, Hou Zhesheng have also developed a kind of fermentor tank (patent No. 200610017078.X) and have wriggled by the fixedly connected flexible wall of the spring assembly of posting piezoelectric patches and realize enhancement of heat transfer, mass transfer, though this fermentor tank can make material wherein produce displacement, but be difficult to amplify, the heat that reaction produces can't in time shed, and is difficult to be applied to industrialization.
Characteristic analysis of the present invention
One aspect of the present invention utilizes solid-state fermenter inwall bottom and lateral air bag cycle to agitate, and makes jar internal solid material produce wriggling, does not produce under the condition of shearing force, reaches mixing effect, can wash by water in the air bag with the control leavening temperature; Gas forms closed cycle by air compressor machine in gas-holder and fermentor tank on the other hand, strengthen the heat and mass of reaction, recycle gas can be that aseptic carbonic acid gas, nitrogen, biogas etc. are applied to anaerobic solid-state fermentation, produce ethanol, butanols, biogas, Succinic Acid, 2, anaerobically fermenting products such as 3-butyleneglycol, recycle gas also can be that sterile air is applied to aerobic solid-state fermentation production microbial oil, zymin, biological pesticide etc.
Summary of the invention
[purpose of the present invention]
The objective of the invention is at solid state fermentation conduct heat, the mass transfer difficulty, stir the difficult problem that energy consumption height and strong mixing influences microorganism growth, provide a kind of employing air bag to agitate and gas circulation flow strengthening solid state fermentation heat transfer, mass transfer the method for strengthening solid state fermentation.
[technical scheme]
Technical scheme of the present invention is as follows:
A kind of apparatus and method of strengthening solid state fermentation, device is by fermentor tank (1), one-level gas-holder (10), secondary gas-holder (101), air compressor machine (11) is formed, fermentor tank (1) is the core component of this device, by tank body, sidewall air bag (2), bottom air bag (3), sidewall air bag intake valve (8), sidewall air bags valve (81), sidewall air bag water intaking valve (82), sidewall air bag water discharge valve (83), bottom air bag intake valve (4), bottom air bags valve (41), bottom air bag water intaking valve (42), bottom air bag water discharge valve (43), tank body intake valve (9), vent valve (6), opening for feed (5), discharge port (7) and temperature control system are formed.
Apparatus of the present invention and method are characterised in that: the inwall bottom and the survey face of fermentor tank all are distributed with the air bag that can heave to fermentor tank inside, bottom air bag and sidewall air bag can form periodically by inflation or water-filling and agitate, on the one hand fermentor tank internal solid material is slowly stirred, strengthen mass-and heat-transfer, on the other hand, water or gas circulation may command leavening temperature in air bag; The fermentor tank bottom has tank body intake valve (9), top that tank body vent valve (6) is arranged, the gas of (10) enters fermentor tank (1) by air compressor machine and fully contacts with fermentation materials in the one-level gas-holder from the bottom, after forming the pressure of 0.01-0.5MPa, keep 2-60s to discharge from vent valve (6) again, get back to gas-holder (10), form closed gas loop cycle, quicken microorganism metabolism, the heat and mass transport mode of solid medium intergranular space gas phase will change the convection current diffusion into by molecular diffusion, strengthen mass transfer, heat transfer.Fermentor tank bottom air bag and fermentor tank bottom are concentric(al) circles, and its radius size is the 1/4-1/2 of fermentor tank radius, is semisphere when heaving fully, and its volume is the 1/20-1/5 of fermentor tank volume, the parallel air bag of a uniform distribution 4-10 tank wall on the inner side-wall.Air bag periodically
Heave and shrink, fermentation materials is pushed lax.Recycle gas can be nitrogen, carbonic acid gas, biogas and air.Apparatus and method of the present invention all adapt to anaerobic solid-state fermentation and aerobic solid-state fermentation.
Description of drawings
Fig. 1 is patent device figure.
Wherein: 1 fermentor tank, 2 sidewall air bags, 3 bottom air bags, 4 bottom air bag intake valves, 41 bottom air bags valves, 42 bottom air bag water intaking valves, 43 bottom air bag water discharge valves, 5 opening for feeds, 6 tank body vent valves, 7 discharge ports, 8 sidewall air bag intake valves, 81 sidewall air bags valves, 82 sidewall air bag water intaking valves, 83 sidewall air bag water discharge valves, 9 tank body intake valves, 10 one-level gas-holder, 101 secondary gas-holder, 11 air compressor machines
Embodiment
Come solid fermentation and straw puffing to produce biogas with apparatus and method of the present invention:
The inventive system comprises fermentor tank (1) as shown in Figure 1, one-level gas-holder (10), secondary gas-holder (101), air compressor machine (11) is formed, fermentor tank (1) is the core component of this device, by tank body, sidewall air bag (2), bottom air bag (3), sidewall air bag intake valve (8), sidewall air bags valve (81), sidewall air bag water intaking valve (82), sidewall air bag water discharge valve (83), bottom air bag intake valve (4), bottom air bags valve (41), bottom air bag water intaking valve (42), bottom air bag water discharge valve (43), tank body intake valve (9), vent valve (6), opening for feed (5), discharge port (7).Reactor features is: the inwall bottom and the survey face of fermentor tank all are distributed with the air bag that can heave to fermentor tank inside, and bottom air bag and sidewall air bag can periodically be agitated; The fermentor tank bottom has tank body intake valve, top that tank body vent valve (6) is arranged, and gas circulates in fermentor tank (1), one-level gas-holder (10) by air compressor machine; Bottom air bag and fermentor tank bottom are concentric(al) circles, and its radius size is 1/2 of a fermentor tank radius, is semisphere when heaving fully, and its volume is 1/5 of a fermentor tank volume, 6 parallel air bags of tank wall of uniform distribution on the inner side-wall.
Carry out steam puffed stalk solid state fermentation biogas as follows with this device: with steam puffed stalk and NH
4HCO
3Mix and regulate C: N to 20-40: 1, insert the active sludge of 30% (W/W), add water and adjust solid-liquid (W/W) than being 1: 3, pH7.0, deployed material is joined in the fermentor tank, start air compressor machine bottom air bag and sidewall air bag are filled air, form and periodically agitate, the cycle of agitating is 10-30 minute; Charge into carbonic acid gas when (10) begin in the one-level gas-holder, carbon dioxide enters fermentor tank (1) by air compressor machine and fully contacts with fermentation materials from the bottom, after forming the pressure of 0.01-0.5MPa, keep 2-60s to discharge from vent valve (6) again, get back to gas-holder (10), the gas circulation cycle is 10-60 minute.Agitate and gas circulation reinforcing mass transfer, heat transfer by periodic air bag, the control leavening temperature is 30-55 ℃, and 3-5 days begin to produce biogas, after solid state fermentation produces biogas, remove part and be used for keeping gas circulation, remaining can discharge utilization from secondary gas-holder (101).
The traditional raw material of biogas is a fowl and animal excrement, but because present aquaculture is also all taked the mode of large-scale cultivation, the enthusiasm that a variety of causes such as grain rise in price cause peasant household to raise livestock and poultry scattered is affected.Because of insufficient raw material has influenced the output of biogas, have because of there not being the fermentation raw material, methane-generating pit is aerogenesis not throughout the year, even the pond phenomenon occurs abandoning.
Therefore, people have invested cheapness to sight
Rich stalk, however stalk fermentation biogas is also unsuccessful at present, has suffered serious setback in engineering practice, mainly have following problem: 1, the combination of stalk cellulose, hemicellulose and xylogen is tight, be difficult to be degraded by microorganisms, cause the aerogenesis cycle long, inefficiency; 2, raw material is easy to swim in above the feed liquid, can not fully contact with microorganism, and slowed down reaction speed, simultaneously, the crust of floating raw material stops biogas in time to be overflowed; 3, adopt the fermentation of traditional pond, cellar mostly, uncontrollable leavening temperature and gas production rate cause the summer aerogenesis many, the few or anaerogenic difficult situation of aerogenesis in winter.
The apparatus and method of the application of the invention, accelerated mass-and heat-transfer, controlled temperature of reaction, biogas output can reach the 500ml/g dry material, and fermentation period is 10-15 days, and cellulosic degradation rate is 75-85%, and do experiment contrast with triangular flask, biogas output is the 234.2ml/g dry material, and fermentation period is 30-35 days, and materials conversion rate is 50-60%.Utilize these apparatus and method to improve the efficient and the stability of stalk fermentation biogas greatly.
Come solid fermentation and straw puffing to produce 2, the 3-butyleneglycol with apparatus and method of the present invention:
Contrive equipment is as described in the embodiment 1, its method is for being to mix at 4: 1 steam puffed stalk and wheat bran with weight ratio, add water and adjust solid-liquid (W/W) than being 1: 3, mix the back 121 ℃ of sterilizations 1 hour, insert the product 2 of 3% (W/W), the seed liquor of 3-butyleneglycol bacterial classification, after join in the fermentor tank, start air compressor machine bottom air bag and sidewall air bag are filled air, form and periodically agitate, the cycle of agitating is 20-40 minute; Charge into carbonic acid gas when (10) begin in the one-level gas-holder, carbon dioxide enters fermentor tank (1) by air compressor machine and fully contacts with fermentation materials from the bottom, after forming the pressure of 0.01-0.3MPa, keep 2-60s to discharge from vent valve (6) again, get back to one-level gas-holder (10), the gas circulation cycle is 10-60 minute.Agitate and gas circulation reinforcing mass transfer, heat transfer by periodic air bag, the control leavening temperature is 30-35 ℃.
The apparatus and method of the application of the invention, accelerated mass-and heat-transfer, controlled temperature of reaction, 2,3-butyleneglycol output can reach the 140g/g dry material, fermentation period is 96 hours, and cellulosic degradation rate is 70-85%, and does experiment contrast with triangular flask, 2,3-butyleneglycol output is the 10g/g dry material, and fermentation period is 112 hours, and materials conversion rate is 60-70%.It is steam explosion stalk fermented 2 to utilize these apparatus and method to improve greatly, the efficient of 3-butyleneglycol and stability.
Claims (6)
1. the device of a strengthening solid state fermentation, device is by fermentor tank (1), one-level gas-holder (10), secondary gas-holder (101), air compressor machine (11) is formed, fermentor tank (1) is the core component of this device, by tank body, sidewall air bag (2), bottom air bag (3), sidewall air bag intake valve (8), sidewall air bags valve (81), sidewall air bag water intaking valve (82), sidewall air bag water discharge valve (83), bottom air bag intake valve (4), bottom air bags valve (41), bottom air bag water intaking valve (42), bottom air bag water discharge valve (43), tank body intake valve (9), vent valve (6), opening for feed (5), discharge port (7) and temperature control system are formed.
2. be characterised in that by the described fermentor tank of claim 1: the inwall bottom and the side of fermentor tank all are distributed with the air bag that can heave to fermentor tank inside, and bottom air bag and sidewall air bag can periodically be agitated; The fermentor tank bottom has tank body intake valve, top that tank body vent valve (6) is arranged, and gas circulates in fermentor tank (1), one-level gas-holder (10) by air compressor machine.
3. be concentric(al) circles by claim 1 described bottom air bag and fermentor tank bottom, its radius size is the 1/4-1/2 of fermentor tank radius, be semisphere when heaving fully, its volume is the 1/20-1/5 of fermentor tank volume, the parallel air bag of a uniform distribution 4-10 tank wall on the inner side-wall.
4. the method for a strengthening solid state fermentation, it is characterized in that, after installing fermentation materials in the fermentor tank (1), close opening for feed (5) and discharge port (7), gas enters fermentor tank (1) by air compressor machine (11) and fully contacts with fermentation materials from the bottom, behind the pressure of formation 0.01-0.5MPa, keep 2-60s to discharge from tank body vent valve (6) again, get back to one-level gas-holder (10), gas circulates in fermentor tank (1), one-level gas-holder (10) by air compressor machine (11); Simultaneously sidewall air bag (2), bottom air bag (3) form by inflation or water-filling and periodically agitate and shrink reinforcing mass transfer, heat transfer.
5. round-robin gas can be nitrogen, carbonic acid gas, biogas and air in fermentor tank (1), one-level gas-holder (10).
6. apparatus and method of the present invention all adapt to anaerobic solid-state fermentation and aerobic solid-state fermentation.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450395A (en) * | 2014-11-29 | 2015-03-25 | 重庆星湖茶酒厂 | Device used for adjusting temperature and air pressure in fermentation tank |
| CN104974926A (en) * | 2015-07-18 | 2015-10-14 | 农业部沼气科学研究所 | Feed device of biogas fermentation tank |
| CN106244440A (en) * | 2016-08-31 | 2016-12-21 | 江苏绿扬生物电子工程有限公司 | A kind of spiral gas-lifting type anaerobic biological reactor |
| CN106615313A (en) * | 2016-11-29 | 2017-05-10 | 湖南惟楚福瑞达生物科技有限公司 | Method and device applicable to processing of eurotium cristatum fermented loose tea |
| CN109423507A (en) * | 2017-09-01 | 2019-03-05 | 中国科学院过程工程研究所 | A kind of method of anaerobic-aerobic mixing fungin butanol solid state fermentation |
| CN110373308A (en) * | 2019-03-21 | 2019-10-25 | 赵海超 | A kind of silage corn simulating lab test device and Silaging method |
| CN110935723A (en) * | 2019-11-18 | 2020-03-31 | 绍兴市中测检测技术股份有限公司 | Degradation device for soil polycyclic aromatic hydrocarbon pollution |
| CN111172199A (en) * | 2018-11-12 | 2020-05-19 | 中国科学院过程工程研究所 | Novel method for solid state fermentation of ethanol reinforced by inert gas |
| CN112980688A (en) * | 2021-02-06 | 2021-06-18 | 湖州师范学院 | Cell bio-genetic engineering is with bio-pharmaceuticals stem cell reaction equipment |
| CN118360129A (en) * | 2024-04-03 | 2024-07-19 | 山东省农业科学院 | Special fermentation tower of food microbial fermentation |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450395A (en) * | 2014-11-29 | 2015-03-25 | 重庆星湖茶酒厂 | Device used for adjusting temperature and air pressure in fermentation tank |
| CN104974926A (en) * | 2015-07-18 | 2015-10-14 | 农业部沼气科学研究所 | Feed device of biogas fermentation tank |
| CN106244440A (en) * | 2016-08-31 | 2016-12-21 | 江苏绿扬生物电子工程有限公司 | A kind of spiral gas-lifting type anaerobic biological reactor |
| CN106615313A (en) * | 2016-11-29 | 2017-05-10 | 湖南惟楚福瑞达生物科技有限公司 | Method and device applicable to processing of eurotium cristatum fermented loose tea |
| CN106615313B (en) * | 2016-11-29 | 2023-08-22 | 湖南惟楚福瑞达生物科技有限公司 | Processing device and method suitable for golden flower loose tea |
| CN109423507A (en) * | 2017-09-01 | 2019-03-05 | 中国科学院过程工程研究所 | A kind of method of anaerobic-aerobic mixing fungin butanol solid state fermentation |
| CN111172199A (en) * | 2018-11-12 | 2020-05-19 | 中国科学院过程工程研究所 | Novel method for solid state fermentation of ethanol reinforced by inert gas |
| CN110373308B (en) * | 2019-03-21 | 2022-08-30 | 赵海超 | Silage corn indoor simulation experiment device and silage method |
| CN110373308A (en) * | 2019-03-21 | 2019-10-25 | 赵海超 | A kind of silage corn simulating lab test device and Silaging method |
| CN110935723A (en) * | 2019-11-18 | 2020-03-31 | 绍兴市中测检测技术股份有限公司 | Degradation device for soil polycyclic aromatic hydrocarbon pollution |
| CN110935723B (en) * | 2019-11-18 | 2021-06-29 | 绍兴市中测检测技术股份有限公司 | Degradation device for soil polycyclic aromatic hydrocarbon pollution |
| CN112980688B (en) * | 2021-02-06 | 2023-03-28 | 湖州学院 | Cell bio-genetic engineering is with bio-pharmaceuticals stem cell reaction equipment |
| CN112980688A (en) * | 2021-02-06 | 2021-06-18 | 湖州师范学院 | Cell bio-genetic engineering is with bio-pharmaceuticals stem cell reaction equipment |
| CN118360129A (en) * | 2024-04-03 | 2024-07-19 | 山东省农业科学院 | Special fermentation tower of food microbial fermentation |
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| CN101798562B (en) | 2013-03-27 |
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