CN101790959A - Method for culturing new dwarf wheat variety - Google Patents

Method for culturing new dwarf wheat variety Download PDF

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Publication number
CN101790959A
CN101790959A CN201010106659A CN201010106659A CN101790959A CN 101790959 A CN101790959 A CN 101790959A CN 201010106659 A CN201010106659 A CN 201010106659A CN 201010106659 A CN201010106659 A CN 201010106659A CN 101790959 A CN101790959 A CN 101790959A
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China
Prior art keywords
seed
individual plant
plant
generation
new
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CN201010106659A
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Chinese (zh)
Inventor
赵凤梧
李慧敏
赵明辉
刘冬成
张爱民
李明哲
郝洪波
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Dry Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences
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Dry Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences
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Priority to CN201010106659A priority Critical patent/CN101790959A/en
Publication of CN101790959A publication Critical patent/CN101790959A/en
Pending legal-status Critical Current

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Abstract

The invention belongs to the technical field of new wheat variety culturing methods and discloses a method for culturing a new dwarf wheat variety. The method for culturing the new dwarf wheat variety is mainly technically characterized by selecting wheat of a variety with an excellent comprehensive property and a huge high yield potential as processing parents and drying the parents in air for 2 days; placing the processing parents in a seed magnetizer with a magnetic field strength of 200 mT and magnetizing the processing parents in the magnetizer for 48 hours to obtain seeds of a M0 generation; planting the seeds to obtain high variable single plants of M1 and M2 generations; planting the single plants which are used as units continuously; performing selfing and purification for two times to obtain a M4 generation; and continuously selecting seeds for cultivation to obtain M5 and M6 generations while the properties such as a heating stage and thousand seed weight are taken into consideration. The method for culturing the new dwarf wheat variety has the advantages that: the plants are obvious lower than the parents; the plant lodging is prevented; the price is low; and pollution and damages to people and environment are avoided.

Description

The breeding method of new dwarf wheat variety
Technical field
The invention belongs to the breeding method technical field of new wheat germplasm, relate in particular to a kind of breeding method of new dwarf wheat variety.
Background technology
The short-stalked variety lodging tolerance is strong, suitable dense planting, and colony's utility rate of luminous energy height, anti-fertilizer, volume increase, the underproduction and the seed processing quality that causes of avoiding lodging reduces.The excavation of short living gene, research and utilization are one of the field of enlivening of biotics section research all the time.The application of dwarf gene makes world wheat output average year increase progressively 3.4%.The wheat the shortest bar gene of extensive utilization is Rht from Norin 10 1, Rht 2With Rht from Akakomugi 8, Rht 9, 50% wheat breed has their short source blood lineage in the world.A kind of resource of short stem of long-term use in the breeding, short living gene is single, and the hereditary basis poorness easily causes catastrophic result.Because long-term large tracts of land is utilized same dwarf gene, to potential because the single risk of bringing of genetic background, cause showing great attention to of genetic breeding worker, and pointed out the still reason of many in recent years short-stalked variety yield potentiality stagnations of newly breeding of dwarf gene unification both at home and abroad.The excavation of short living gene and utilization research more and more come into one's own.Further create the new germ plasm resource of short stem of wheat by genetic improvement, will directly promote the raising of wheat genetic breeding level.Most irradiation treatment and the chemicalss of adopting of the acquisition of the short living gene of wheat are at present induced, and impel its producer sudden change.Pollute greatly problems such as cost height but all exist.
Summary of the invention
The problem to be solved in the present invention just provide a kind of cheap, to the breeding method of the new dwarf wheat variety of people and environmental nonpollution, nothing injury.
For addressing the above problem, the technical solution used in the present invention is: the breeding method of new dwarf wheat variety is characterized in that: this breeding method may further comprise the steps:
The first step: the parent selects
Select comprehensive proterties good, the wheat breed that the high yield potentiality are big is the processing parent;
Second step: select seed
4 days prior to seeding, the seed that is about to handle is carried out hand picking, select the high seed of plumpness, the seed of selecting was carried out airing 2 days;
The 3rd step: seed is handled in magnetization
It is that the seed magnetization machine of 200mT magnetizes and handled 48 hours that the seed of select is put into magnetic field intensity; Obtain M 0The generation seed;
The 4th step: plant and obtain M 1The variation individual plant
M with the magnetization processing 0Carry out the simple grain program request for seed by spacing in the rows 10cm, line-spacing 20cm; Obtain M 1In generation, select plant height M 1Generation variation individual plant is that unit gathers in the crops, threshing with the individual plant;
The 5th step: plant and obtain M 2The variation individual plant
The 4th step was obtained M 1Individual plant is that unit carries out the simple grain program request by spacing in the rows 10cm, line-spacing 20cm with the individual plant, obtains M 2In generation, select M 2Short bar variation of generation individual plant is unit results, threshing with the individual plant;
The 6th step: plant and obtain M 4Short bar variation individual plant
Step into the individual plant M of short stem of choosing with the 5th 2, continuing with the individual plant is that unit plants, selfing purifying 2 times is to M 4Generation, take into account proterties such as heading stage, thousand kernel weight simultaneously, with the individual plant unit results, threshing;
The 7th step: continue directive breeding and obtain M 5Short bar variation individual plant
The 6th step is obtained seed continue directive breeding, obtain M 5Generation is unit results, threshing with the individual plant;
The 8th step: continue cultivation and obtain M 6Short bar strain is
The 7th step is obtained seed continue seed selection, obtain M 6Generation, identify that strain is a property stability, the stablize of short stem strain good to economical character is to mix results;
The 9th step: obtain new germ plasm
The 8th step is obtained seed carry out numerous kind, obtain the dwarf wheat new germ plasm.
The breeding method of new dwarf wheat variety provided by the present invention compared with prior art has the following advantages: one is that the seed magnetization machine of 200mT magnetizes and handled 48 hours because the seed of select is put into magnetic field intensity, obtains M 0The generation seed; M 0Seed is through field planting, the M that obtains 1In generation, plant height takes place and downgrades variation in a part, and this variation proterties can entail the next generation; Like this, through the cultivation and the screening of several generations, can obtain stable plant height and downgrade new germ plasm;
Use said method, create 5 new germ plasms of short stem, plant height 53.32-58.38cm, the economical character performance sees the following form 1.
The short living new germ plasm The Characters of table 1
Title Plant height The strain spike number Spike length Spikelet number Grain number per spike Thousand kernel weight
New 1 ??53.32 ??9.4 ??7.63 ??17.80 ??43.4 ??40.9
New 2 ??58.26 ??9.6 ??7.94 ??17.67 ??45.33 ??41.2
New 3 ??60.31 ??7.3 ??8.12 ??17.14 ??40.71 ??40.1
New 4 ??57.22 ??6.4 ??7.95 ??17.50 ??42.83 ??40.6
New 5 ??58.38 ??9.4 ??7.93 ??17.29 ??41.71 ??42.9
Handle parent Handan 6172 ??66.78 ??9.8 ??8.40 ??18.3 ??47.60 ??47.2
Above-mentioned 5 new germ plasms, plant height is compared with the mutagenesis parent, 13.46cm, 8.52cm, 6.47cm, 9.56cm and 8.40cm have been reduced respectively, the reduction amplitude is respectively 20.16%, 12.67%, 9.69%, 14.32% and 12.58%, significance test is analyzed, and plant height is compared difference with the parent and all reached utmost point significance level.
Embodiment
Below in conjunction with embodiment the breeding method of new dwarf wheat variety of the present invention is described in further details.
Embodiment
This breeding method may further comprise the steps:
The first step: the parent selects
Select comprehensive proterties good, the big wheat breed of high yield potentiality is for handling the parent: select that comprehensive proterties is good, wide adaptability, common wheat (T.aestivum) kind " Handan 6172 " that the high yield potentiality are big be for handling the parent, creating for new germ plasm provides hereditary basis.This kind is that national the Yellow River and Huai He River north panel region is tested secondary check variety successively by Shanxi, Ji, Lu San province and the Yellow River and Huai He River south sheet, the authorization of the Yellow River and Huai He River north sheet two biomes country at present.Certainly, also can adopt other comprehensive proterties good, the big wheat breed of high yield potentiality.
Second step: select seed
Four days prior to seeding, the seed that is about to handle is carried out hand picking, select 5000 in the high seed of plumpness, and the seed of selecting was carried out airing 2 days;
The 3rd step: seed is handled in magnetization
With select and airing after 5000 seeds to put into magnetic field intensity be that the seed magnetization machine of 200mT magnetizes and handled 48 hours, obtain M 0For seed;
The 4th step: plant and obtain M 1For plant height variation individual plant
M with the magnetization processing 0Carry out the simple grain program request for seed by spacing in the rows 10cm, line-spacing 20cm, the long 8m of row; Obtain M 1In generation, the high variation of selected plant individual plant is that unit gathers in the crops, threshing with the individual plant, M 1120 strains are selected in substitution;
The 5th step: plant and obtain M 2For variation individual plant of short stem
The 4th step was obtained M 1For individual plant, be that unit carries out the simple grain program request by spacing in the rows 10cm, line-spacing 20cm, the long 8m of row with the individual plant, obtain M 2Generation, select variation individual plant of short stem, with the individual plant unit results, threshing, M 2130 strains are selected in substitution;
The 6th step: plant and obtain M 4For variation individual plant of short stem
Step into the M of choosing with the 5th 2Generation individual plant of short stem, continuing with the individual plant is that unit plants, selfing purifying 2 times is to M 4Generation, take into account proterties such as heading stage, thousand kernel weight simultaneously, with the individual plant unit results, threshing, M 360 strains, M are selected in substitution 430 strains are selected in substitution;
The 7th step: continue directive breeding and obtain M 5For variation individual plant of short stem
The seed that the 6th step was obtained continues directive breeding, obtains M 5Generation is unit results, threshing with the individual plant, M 520 strains are selected in substitution;
The 8th step: continue cultivation and obtain M 6Generation strain of short stem system
The seed that the 7th step was obtained continues seed selection, obtains M 6Generation, identify that strain is a property stability, the stablize of short stem strain good to economical character is to mix results, in conjunction with indoor measurement to other proterties, M 6For 5 the strains systems of selecting and remain;
The 9th step: obtain new germ plasm
The seed that the 8th step was obtained carries out numerous kind, obtains new dwarf wheat variety.
Use said method, create 5 new germ plasms of short stem, plant height 53.32-58.38cm, the economical character performance sees the following form 1.
The short living new germ plasm The Characters of table 1
Title Plant height The strain spike number Spike length Spikelet number Grain number per spike Thousand kernel weight
New 1 ??53.32 ??9.4 ??7.63 ??17.80 ??43.4 ??40.9
New 2 ??58.26 ??9.6 ??7.94 ??17.67 ??45.33 ??41.2
Title Plant height The strain spike number Spike length Spikelet number Grain number per spike Thousand kernel weight
New 3 ??60.31 ??7.3 ??8.12 ??17.14 ??40.71 ??40.1
New 4 ??57.22 ??6.4 ??7.95 ??17.50 ??42.83 ??40.6
New 5 ??58.38 ??9.4 ??7.93 ??17.29 ??41.71 ??42.9
Handle parent Handan 6172 ??66.78 ??9.8 ??8.40 ??18.3 ??47.60 ??47.2
Above-mentioned 5 new germ plasms, plant height is compared with the mutagenesis parent, 13.46cm, 8.52cm, 6.47cm, 9.56cm and 8.4cm have been reduced respectively, the reduction amplitude is respectively 20.16%, 12.67%, 9.69%, 14.32% and 12.58%, significance test is analyzed, and plant height is compared difference with the parent and all reached utmost point significance level.

Claims (1)

1. the breeding method of new dwarf wheat variety, it is characterized in that: this breeding method may further comprise the steps:
The first step: the parent selects
Select comprehensive proterties good, the wheat breed that the high yield potentiality are big is the processing parent;
Second step: select seed
4 days prior to seeding, the seed that is about to handle is carried out hand picking, select the high seed of plumpness, the seed of selecting was carried out airing 2 days;
The 3rd step: seed is handled in magnetization
It is that the seed magnetization machine of 200mT magnetizes and handled 48 hours that the seed of select is put into magnetic field intensity; Obtain M 0The generation seed;
The 4th step: plant and obtain M 1For plant height variation individual plant
M with the magnetization processing 0Carry out the simple grain program request for seed by spacing in the rows 10cm, line-spacing 20cm; Obtain M 1Generation, the high variation of selected plant M 1For individual plant, be that unit gathers in the crops, threshing with the individual plant;
The 5th step: plant and obtain M 2Short bar variation of generation individual plant
The 4th step was obtained M 1Individual plant is that unit carries out the simple grain program request by spacing in the rows 10cm, line-spacing 20cm with the individual plant, obtains M 2In generation, the short bar individual plant of the high variation of selected plant is unit results, threshing with the individual plant;
The 6th step: plant and obtain M 4The variation individual plant
Step into the M of choosing with the 5th 2Individual plant of short stem, continuing with the individual plant is that unit plants, selfing purifying 2 times is to M 4Generation, take into account proterties such as heading stage, thousand kernel weight simultaneously, with the individual plant unit results, threshing;
The 7th step: continue directive breeding and obtain M 5Short bar variation individual plant
The 6th step is obtained seed continue directive breeding, obtain M 5Generation is unit results, threshing with the individual plant;
The 8th step: continue cultivation and obtain M 6Short bar strain is
The 7th step is obtained seed continue seed selection, obtain M 6Generation, identify that strain is a property stability, the stablize of short stem strain good to economical character is to mix results;
The 9th step: obtain new germ plasm
The 8th step is obtained seed carry out numerous kind, obtain the dwarf wheat new germ plasm.
CN201010106659A 2010-02-02 2010-02-02 Method for culturing new dwarf wheat variety Pending CN101790959A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103503769A (en) * 2013-10-18 2014-01-15 河北省农林科学院旱作农业研究所 Novel wheat mutation breeding method
CN106718487A (en) * 2016-11-16 2017-05-31 普定县科技服务中心 A kind of method for culturing seedlings of Chinese yew
CN114145198A (en) * 2021-10-21 2022-03-08 潘捷文 Cultivation method of new short-stalk high-yield wheat variety

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103503769A (en) * 2013-10-18 2014-01-15 河北省农林科学院旱作农业研究所 Novel wheat mutation breeding method
CN106718487A (en) * 2016-11-16 2017-05-31 普定县科技服务中心 A kind of method for culturing seedlings of Chinese yew
CN114145198A (en) * 2021-10-21 2022-03-08 潘捷文 Cultivation method of new short-stalk high-yield wheat variety

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Application publication date: 20100804