CN101775752A - Method for pretreating Lyocell fiber by using cellulase before pulping - Google Patents
Method for pretreating Lyocell fiber by using cellulase before pulping Download PDFInfo
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- CN101775752A CN101775752A CN201010022935A CN201010022935A CN101775752A CN 101775752 A CN101775752 A CN 101775752A CN 201010022935 A CN201010022935 A CN 201010022935A CN 201010022935 A CN201010022935 A CN 201010022935A CN 101775752 A CN101775752 A CN 101775752A
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Abstract
The invention provides a method for pretreating Lyocell fiber by using cellulase before pulping, which is characterized by comprising the following steps: mixing the Lyocell fiber and the cellulase in a weight ratio of 100: 1-3, adjusting the concentration of pulp to between 2 and 5 weight percent by using water, adjusting the pH value to between 4.5 and 6, and treating the mixture for 30 to 60 minutes at the temperature of between 45 and 60 DEG C; and performing pulping treatment on the obtained enzyme treated material in a PFI mill, wherein water is not added during pulping. The method has the advantages that because the Lyocell fiber is pretreated by using the cellulase before pulping, the swelling of the fiber becomes softer, the pulping time is reduced, the fibrillating degree of the fiber is greatly improved, and the length of the fiber is kept; and moreover, the alkali resistance of the fiber is improved.
Description
Technical field
The present invention relates to a kind of cellulase that utilizes and carry out pretreated Lyocell fiber methods of beating.
Background technology
The Lyocell fiber is a kind of regenerated celulose fibre of novel, environmental protection, has typical skin-core structure, and mainly by parallel the rearranging of huge fibril height, therebetween has the amorphous region to sandwich layer, and fibrillar size is all at nanoscale.The Lyocell fiber is easy to generate the fibrillation structure, for some artificial fibres and synthetic fiber, fibrillation is meant that fiber is when being subjected to mechanical force, the fiber top layer of otherwise smooth is damaged, the fibril of fibrous inside or fibril bundle are exposed, invest on the fibrous body, also have part to break away from fibrous body.
Handle the fibrillation structure that the generation of Lyocell fiber is enriched by the machinery making beating, the Lyocell fiber of fibrillation is particularly suitable for the preparation of some speciality paper, can improve paper tear intensity, opacity well, adjusts paper permeability.Utilize the fibrillation structure, also suitable preparation air permeability is big, the aperture is little and the paper of porous nickel, as high accuracy filter material, battery diaphragm etc.In the prior art, preliminary treatment is not carried out in Lyocell fiber making beating, causes easily that beating time is long, the original fiber degree of fiber is low, characteristics such as the beatability of fiber and alkaline resistance properties difference.
Cellulase is a kind of of enzyme, plays biocatalysis when decomposition of cellulose.Cellulase reaction and general enzyme reaction are different, and its main difference is that cellulase is a multicomponent enzyme system, and substrate structure is extremely complicated.Because the ES compound process that the water-insoluble of substrate, REACTION OF ADSORPTION OF CELLULASE have replaced enzyme-to-substrate to form.Cellulase is adsorbed on the substrate cellulose earlier specifically, under the synergy of several components cellulose is resolved into glucose then.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing biology enzyme that the Lyocell fiber is pulled an oar and handled, improve the beatability and the alkaline resistance properties of Lyocell fiber.
In order to achieve the above object, technical scheme of the present invention provides a kind of cellulase that utilizes and carries out pretreated Lyocell fiber methods of beating, it is characterized in that concrete steps are:
The first step: with Lyocell fiber and enzyme activity level be the cellulase of 1000-1600IU/g by weight 100: 1-3 mixes, and it is 2wt%～5wt% that water is regulated slurry concentration, adjusting pH value to 4.5～6,45～60 ℃ of temperature, processing time 30～60min;
Second step: the processing of pulling an oar is expected in the processing of first step gained enzyme in the PFI mill, pulping process does not add water.
In described second step making beating processing procedure, abrasive disk space is controlled at 0.2mm.
Principle of the present invention is: Lyocell fiber molecule interchain is mainly by hydrogen bonded, and hydrone can only enter the amorphous region of fiber, destroys hydrogen bond and causes the fiber limited swelling.Because the interior strand of crystal region is arranged in parallel and leans on very closely, so hydrone can not enter wherein.But after cellulase was to cellulose hydrolysis, fiber is swelling more significantly, and hydrone enters crystal region, helped the making beating and the wire-dividing broom purification of fiber; The hydrogen bond that forms once more after enzyme is handled is extremely stable.
The present invention adopts the preliminary treatment before cellulase is pulled an oar to the Lyocell fiber, makes fibre swelling become soft more, reduces beating time, has increased substantially the original fiber degree of fiber, helps keeping fibre length; But also improved the alkaline resistance properties of fiber.
The specific embodiment
Specify the present invention below in conjunction with embodiment.
Embodiment 1
(1) enzyme mixes and the effect stage: Lyocell fiber (Austrian Lenzin fiber company, 1.7D*4mm) add with enzyme activity be cellulase (the neutral cellulase Cellusoft CR of 1000IU/g, Novozymes company produces) 1.0% (weight ratio), add entry, make that slurry concentration is 2.0wt%, fiber and biology enzyme evenly mix.PH value to 4.5～6 of regulating system, temperature are controlled at 45 ℃, processing time 30min.
(2) with the biology enzyme immixture after the slurry fibrillation of in PFI mill, pulling an oar handle, abrasive disk space is controlled at 0.2mm, pulping process does not add water.
The above-mentioned Lyocell fiber that obtains, weight-loss ratio 0.44%, fibre length 1.83mm, the alkali loss rate 0.7% handled.
Embodiment 2
(1) enzyme mixes and the effect stage: Lyocell fiber (Austrian Lenzin fiber company, 1.7D*4mm) to add enzyme activity be cellulase (the neutral cellulase Cellusoft CR of 1200IU/g, Novozymes company produces) 2.0% (weight ratio), add entry, make that slurry concentration is 3.0wt%, fiber and biology enzyme evenly mix.PH value to 4.5～6 of regulating system, temperature are controlled at 50 ℃, processing time 40min.
(2) with the biology enzyme immixture after the slurry fibrillation of in PFI mill, pulling an oar handle, abrasive disk space is controlled at 0.2mm, pulping process does not add water.
The above-mentioned Lyocell fiber that obtains, weight-loss ratio 1.0%, fibre length 1.95mm, the alkali loss rate 0.5% handled.
Embodiment 3
(1) enzyme mixes and the effect stage: Lyocell fiber (Austrian Lenzin fiber company, 1.7D*4mm) to add enzyme activity be cellulase (the neutral cellulase Cellusoft CR of 1600IU/g, Novozymes company produces) 3.0% (weight ratio), add entry, make that slurry concentration is 5.0wt%, fiber and biology enzyme evenly mix.PH value to 4.5～6 of regulating system, temperature are controlled at 60 ℃, processing time 60min.
(2) with the biology enzyme immixture after the slurry fibrillation of in PFI mill, pulling an oar handle, abrasive disk space is controlled at 0.2mm, pulping process does not add water.
The above-mentioned Lyocell fiber that obtains, weight-loss ratio 2.0%, fibre length 2.07mm, the alkali loss rate 0% handled.
Without the biology enzyme preliminary treatment with pull an oar respectively under the same conditions through the pretreated Lyocell fiber of 2.0% biology enzyme, the performance indications of gained slurry are as shown in the table:
| Without the biology enzyme preliminary treatment | Biology enzyme preliminary treatment (addition 2.0%) | |
| Starch dense/% | ??3.0％ | ??3.0％ |
| The making beating mode | The PFI mill | The PFI mill |
| Weight-loss ratio/% | ??0.9 | ??1.0 |
| Without the biology enzyme preliminary treatment | Biology enzyme preliminary treatment (addition 2.0%) | |
| Fibre length/mm | ??1.32 | ??1.95 |
| Alkali loss rate/% | ??4.0 | ??0.5 |
Cellulase activity assay method among the embodiment 1-3 is as follows:
Get 1mL enzyme liquid and add 1mL0.1molL
-1, pH4.6 acetate buffer solution, be preheating to 50 ℃, add 1 1x6cm Xinhua filter paper (50+1mg), 50 ℃ the insulation 1h.Take out, boiling water bath deactivation 5min is cooled to room temperature, with 3 times of dilutions behind the 3mLDNS reagent colour development, surveys OD value (520nm).
Enzyme activity calculates: find glucose μ mol number from calibration curve;
Enzyme activity (μ/mL)=glucose amount/60 * Ew
Wherein: 60 be temperature retention time (enzyme-to-substrate action time, min);
Ew is the volume (mL) of crude enzyme liquid;
U is meant that under given conditions the per minute catalyzing cellulose hydrolysis becomes the enzyme amount of 1 μ mol glucose.
The assay method of the Lyocell fiber alkali loss rate among the embodiment 1-3 is as follows:
Get the Lyocell fiber of certain mass, at 105 ℃ thermostatic drying chamber inner drying 2h, weighing quality m1, in the electrolyte of 6mol/LKOH+15g/LLiOH, soak 4h again, with washed with de-ionized water until neutrality, then at 105 ℃ thermostatic drying chamber inner drying 2h, the m of weighing quality again
2The alkali loss rate is calculated as follows:
Alkali loss rate=[(m
1-m
2)/m
1] * 100%.
Claims (2)
1. one kind is utilized cellulase to carry out pretreated Lyocel l fiber methods of beating, it is characterized in that concrete steps are:
The first step: with Lyocell fiber and enzyme activity level be the cellulase of 1000-1600IU/g by weight 100: 1-3 mixes, and it is 2wt%～5wt% that water is regulated slurry concentration, adjusting pH value to 4.5～6,45～60 ℃ of temperature, processing time 30～60min;
Second step: the processing of pulling an oar is expected in the processing of first step gained enzyme in the PFI mill, pulping process does not add water.
2. the cellulase that utilizes as claimed in claim 1 carries out pretreated Lyocell fiber methods of beating, it is characterized in that, in described second step making beating processing procedure, abrasive disk space is controlled at 0.2mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010022935A CN101775752A (en) | 2010-01-19 | 2010-01-19 | Method for pretreating Lyocell fiber by using cellulase before pulping |
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| CN201010022935A CN101775752A (en) | 2010-01-19 | 2010-01-19 | Method for pretreating Lyocell fiber by using cellulase before pulping |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN111996830A (en) * | 2020-09-04 | 2020-11-27 | 江苏好健康新材料有限公司 | Production process of fiber composite material household paper |
-
2010
- 2010-01-19 CN CN201010022935A patent/CN101775752A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN103821022B (en) * | 2014-03-11 | 2016-02-03 | 山东尤特尔生物科技有限公司 | The method of neutral cellulase preliminary treatment needle slurry and broad-leaved slurry |
| CN111996830A (en) * | 2020-09-04 | 2020-11-27 | 江苏好健康新材料有限公司 | Production process of fiber composite material household paper |
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Application publication date: 20100714 |