CN101775358A - Accharomyces cerevisiae and application thereof in adsorption of aflatoxin - Google Patents
Accharomyces cerevisiae and application thereof in adsorption of aflatoxin Download PDFInfo
- Publication number
- CN101775358A CN101775358A CN201010109125A CN201010109125A CN101775358A CN 101775358 A CN101775358 A CN 101775358A CN 201010109125 A CN201010109125 A CN 201010109125A CN 201010109125 A CN201010109125 A CN 201010109125A CN 101775358 A CN101775358 A CN 101775358A
- Authority
- CN
- China
- Prior art keywords
- saccharomyces cerevisiae
- adsorption
- aflatoxin
- thalline
- lymt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses accharomyces cerevisiae and an application thereof in adsorption of aflatoxin B1. The bacterial strain is accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382. After being fermented, the bacterial strain produces thallus which has the adsorption on the aflatoxin B1. The test of the adsorption of accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382 on the aflatoxin B1 in a culture medium shows that the adsorption rate of the thallus of the accharomyces cerevisiae on the aflatoxin B1 reaches 81.16%; and the test of the adsorption of accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382 on the aflatoxin B1 in a peanut butter shows that the adsorption rate of the thallus of the accharomyces cerevisiae on the aflatoxin B1 in the peanut butter reaches 88.32%.
Description
Technical field
The present invention relates to an Accharomyces cerevisiae and application thereof, particularly relate to yeast saccharomyces cerevisiae and the application in adsorption of aflatoxin thereof.
Background technology
Aflatoxin (Aflatoxin AFT) is flavus (A.flavus), the mould mycetogenetic secondary metabolites such as (A.nomius) in Aspergillus parasiticus (A.parasiticus) and Tequ, and common have an AFB
1, AFB
2, AFG
1, AFG
2, AFM
1, AFM
2, AFB
2a, AFG
2a, AFBM
2aAnd AFGM
2aDeng, AFB wherein
1(AFB
1) toxicity the strongest.A large amount of epidemiology surveys and research all confirm AFB
1Can bring out the foundation of human liver cell JEG-3 with the hepatitis B virus synergy.AFB
1Also can influence animal embryo, cause immunosuppression and repeated infection, reduce giving milk and egg productivity of some animal.Aflatoxin mainly is present in corn, in the foods such as peanut, has a strong impact on the outlet of China's peanut and goods thereof; In addition, feed also often is subjected to the pollution of aflatoxin.Therefore, the poison-removing method that presses for a kind of highly effective and safe is eliminated the harm of this toxoid to human and livestock health.
At present, the biology poison-removing method of aflatoxin mainly contains following several:
1, the enzyme liberating aflatoxin that produces of microbial metabolism: for example from Nocardia bacteria DSM 12676, have a liking for the degrading enzyme that extracts the narrow food Zymomonas mobilis of wheat, Armillariella tabescens, mycobacterium DSM 44556T, Rhodococcus and the oyster cap fungus, this method degradation rate is higher, but enzyme is subject to pH, condition effect such as temperature and inactivation, in addition, method also not studies have shown that at present the security of aflatoxin degraded product, so may produce secondary pollution.
2, microbial cells itself and cell wall extracts adsorption of aflatoxin thereof: for example lactobacillus rhamnosus GG, lactobacillus rhamnosus LC-705, lactobacterium casei cheese subspecies CGMCC 1.539 and some yeast saccharomyces cerevisiae, above-mentioned bacterial strains can combine with aflatoxin by physics mode, form toxin-thalline mixture, easy and the food separation of this type of mixture can reach and remove AFB in the food
1Purpose.This method is easy, economical, can not produce secondary pollution, has development prospect preferably.
Summary of the invention
The purpose of this invention is to provide an Accharomyces cerevisiae, this bacterial strain thalline itself can adsorption of aflatoxin, and this thalline can be used for preparing the biotechnological formulation of adsorption of aflatoxin, is used for the biological detoxication of aflatoxin.
Yeast saccharomyces cerevisiae provided by the present invention is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1.
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 2nd, 2009 and (is called for short CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation registration number is: CGMCC № 3382.
S. cervisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is that dough from traditional spontaneous fermentation of being used for making steamed bun separates and obtains.
Another object of the present invention provides a kind of adsorption of aflatoxin B
1Biotechnological formulation.
Adsorption of aflatoxin B provided by the present invention
1Biotechnological formulation, be the thalline that ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 obtains.
Described thalline is to obtain after the centrifugal removal supernatant liquor of fermented liquid with yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
In the above-mentioned biotechnological formulation, the fermention medium of described ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 consists of: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water; Described percentage composition is the quality percentage composition;
The pH value of described fermention medium is 7.0-7.2.
Described fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, shaking table rotation radius 20mm～26mm, shaking culture 30h～34h.
The thalline of described acquisition is also through 121 ℃ of heating.The step of described heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer (pH6.0) again at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline.
The 3rd purpose of the present invention provides above-mentioned adsorption of aflatoxin B
1The preparation method of biotechnological formulation.
Adsorption of aflatoxin B provided by the present invention
1The preparation method of biotechnological formulation, be ferment wine brewing yeast S.cerevisiae) LYMT-Y1 CGMCC № 3382, from its fermented liquid, separate the thalline that obtains and be adsorption of aflatoxin B
1Biotechnological formulation.
Among the above-mentioned preparation method, the fermention medium of described ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382 consists of: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water; Described percentage composition is the quality percentage composition;
The pH value of described fermention medium is 7.0-7.2.
Described fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, shaking table rotation radius 20mm～26mm, shaking culture 30h～34h.
The thalline of described acquisition is also through 121 ℃ of heating.The step of described heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer (pH6.0) again at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline.
Ferment wine brewing yeast of the present invention (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 and above-mentioned biotechnological formulation are at adsorption of aflatoxin B
1In application also belong to protection scope of the present invention.
In the described application, described biotechnological formulation or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382 adsorption of aflatoxin B
1Temperature condition be 20-30 ℃, be preferably 23 ℃; The time of described absorption is 30-120min, preferred 30min.In order to reach the good adsorption effect, as required, the addition of described yeast saccharomyces cerevisiae (S.cerevisiae) LYMT-Y1 CGMCC № 3382 can be 2.5 * 10
9Individual yeast cell/ml treats more than the absorption system, in order to save cost, raises the efficiency, and the addition of described yeast saccharomyces cerevisiae can be 2.5 * 10
9-1.7 * 10
10Individual yeast cell/ml treats absorption system.
Experiment showed, 3382 pairs of AFBs of yeast saccharomyces cerevisiae of the present invention (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Has very strong adsorptive power.AFB in yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) the LYMT-Y1 absorption substratum
1Experiment show, when the thalline addition is 1.7 * 10
10Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 30 ℃, and adsorption time is 2h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 94.32%, AFB
1Concentration be reduced to 1.31ng/g from 23.11ng/g; When the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 25 ℃, and adsorption time is 1h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 81.16%, AFB
1Concentration be reduced to 3.76ng/g from 19.95ng/g.AFB in yeast saccharomyces cerevisiae (S.cerevisiae) the LYMT-Y1 absorption peanut paste
1Experiment show that the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 23 ℃, and adsorption time is 30min, and shaking speed is 200rpm, and the thalline of yeast saccharomyces cerevisiae is to AFB in the peanut paste
1Adsorption rate reach 88.32%, AFB in the peanut paste
1Concentration be reduced to 2.63ng/g from 22.50ng/g.
Therefore, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 and with its preparation biotechnological formulation at AFB
1Removal in have a good application prospect.
Description of drawings
Fig. 1 is the concrete form of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
Fig. 2 is the flat-plate bacterial colony form of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1.
Embodiment
Experimental technique described in the following embodiment if no special instructions, is ordinary method; Described reagent and biomaterial if no special instructions, all can obtain from commercial channels.
Realize that substratum of the present invention is specific as follows described:
Yeast (YPD) substratum: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water, described percentage composition is the quality percentage composition.The agar that adds 1.5% quality percentage composition in the YPD liquid nutrient medium promptly obtains the YPD solid medium.
Separation, purifying and the evaluation of embodiment 1, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
In March, 2009, in Bechtop, to take and be placed in traditional spontaneous fermentation dough of making steamed bun that vibration 15min prepares bacteria suspension in the sterile distilled water, shaking speed is 180rpm; Bacteria suspension is coated on the YPD culture medium flat plate after with the sterilized water doubling dilution, cultivates under 25 ℃ of conditions, behind about 40h, bacterium colony is covered with whole flat board, places the YPD liquid nutrient medium with several the different bacterium colonies on the transfering loop picking flat board, and 30h is cultivated in concussion.The thalline that centrifugal above-mentioned fermented liquid is obtained is applied to AFB then
1Adsorption test, thus filter out AFB
1The bacterial strain that adsorptive power is the strongest.Dyed back is observed under optical electron microscope and the Physiology and biochemistry experiment determines that this bacterial strain is a yeast saccharomyces cerevisiae.The above-mentioned bacterial strain that is defined as yeast saccharomyces cerevisiae carried out enlarged culturing and with its called after LYMT-Y1.
According to saccharomycetic feature and identification handbook (English Barnett, work such as J.A.; Hu Ruiqing translates, press of Qingdao Marine University, 1991.6) the middle method of describing, bacterial strain LYMT-Y1 CGMCC № 3382 is carried out morphological specificity, cultural characters and physio-biochemical characteristics identify that concrete outcome is as follows:
(1) morphological specificity of thalline
This strain cell ovalize, gemmation, each cell produces one or more gemma, and gemma is ovalization also.The concrete form of this strain cell is seen Fig. 1.
(2) cultural characters
Bacterium colony is creamy white, smooth, circular, neat in edge, colony diameter 3-4mm, aerobic, bacterium colony is soft and moistening, the even picking easily of quality.The flat-plate bacterial colony of this bacterial strain is cultivated form and is seen Fig. 2.
(3) physio-biochemical characteristics
Fermentation :+; Nitrate :-; The urine enzyme :-; The blue B of diazo (DBB) experiment :-.Annotate: "+" expression positive reaction, "-" expression negative reaction.
Based on above feature, bacterial strain LYMT-Y1 is accredited as yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), and be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 2nd, 2009 and (be called for short CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation registration number is CGMCC № 3382.
AFB in embodiment 2,3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 1
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, and 30 ℃, rotation radius 20mm is behind the 180rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration, get an amount of fermented liquid according to count results, the centrifugal then yeast saccharomyces cerevisiae thalline that obtains, above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0), at 121 ℃ of heating 30min, centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was finished.
Pipette 2ml YPD substratum, detect through high performance liquid chromatography and obtain AFB in this substratum
1Concentration be 23.11 ± 0.87ng/g, this substratum is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 1.7 * 10
10Individual yeast cell/ml, 30 ℃ of adsorption temps are set, adsorption time 2h, shaking speed 200rpm, after effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the substratum of detoxification with the mixing solutions of above-mentioned thalline and substratum, detect AFB in the detoxification substratum through high performance liquid chromatography
1Concentration be 1.31 ± 0.10ng/g, this thalline is to AFB
1Adsorption rate reach 94.32 ± 0.43%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple mean values.
Reference examples 1
Pipette 2ml YPD substratum same as described above, detect and obtain AFB in this substratum
1Concentration be 23.11 ± 0.87ng/g, this substratum is added in the blank centrifuge tube, 30 ℃ of operative temperatures are set, action time 2h, shaking speed 200rpm, the effect finish after, with substratum 10, the centrifugal 10min of 000rpm collects upper solution, detects AFB in the substratum through high performance liquid chromatography
1Concentration be 23.11 ± 0.87ng/g.
Experimental result shows, 3382 pairs of AFBs of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, when the thalline addition is 1.7 * 10
10Individual yeast cell/ml, thalline through 121 ℃ the heating 30min, 30 ℃ of adsorption temps, adsorption time 2h, during shaking speed 200rpm, this thalline is to AFB in the substratum
1Adsorption rate reach 94.32 ± 0.43%, can make AFB in the substratum
1Concentration be reduced to 1.31 ± 0.10ng/g from 23.11 ± 0.87ng/g.
AFB in embodiment 3,3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 2
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, 25 ℃ of cultivations, rotation radius 26mm is behind the 200rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration, get an amount of fermented liquid according to count results, the centrifugal then yeast saccharomyces cerevisiae thalline that obtains, above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0) at 121 ℃ of heating 30min, and centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was finished.
Pipette 2ml YPD substratum, detect through high performance liquid chromatography and obtain AFB in this substratum
1Concentration be 19.95 ± 0.30ng/g, this substratum is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 2.5 * 10
9Individual yeast cell/ml, 25 ℃ of adsorption temps are set, adsorption time 1h, shaking speed 200rpm, after effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the substratum of detoxification with the mixing solutions of above-mentioned thalline and substratum, detect AFB in the detoxification substratum through high performance liquid chromatography
1Concentration be 3.76 ± 0.05ng/g, this thalline is to AFB
1Adsorption rate reach 81.16 ± 0.26%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple mean values.
Reference examples 2
Pipette 2ml YPD substratum same as described above, detect and obtain AFB in this substratum
1Concentration be 19.95 ± 0.30ng/g, this substratum is added in the blank centrifuge tube, 25 ℃ of operative temperatures are set, action time 1h, shaking speed 200rpm, the effect finish after, with substratum 10, the centrifugal 10min of 000rpm collects upper solution, detects AFB in the substratum through high performance liquid chromatography
1Concentration be 19.95 ± 0.30ng/g.
Experimental result shows, AFB in 3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, thalline through 121 ℃ the heating 30min, 25 ℃ of adsorption temps, adsorption time 1h, during shaking speed 200rpm, this thalline is to AFB
1Adsorption rate reach 81.16 ± 0.26%, can make AFB in the substratum
1Concentration be reduced to 3.76 ± 0.05ng/g from 19.95 ± 0.30ng/g.
After handling according to the method for the foregoing description 2-3 and Comparative Examples 1-2, AFB in the substratum
1Concentration all significantly reduced.AFB in 3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test show, when the thalline addition is 1.7 * 10
10Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 30 ℃, and adsorption time is 2h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB in the substratum
1Adsorption rate reach 94.32%, AFB
1Concentration be reduced to 1.31ng/g from 23.11ng/g; When the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 25 ℃, and adsorption time is 1h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 81.16%, AFB
1Concentration be reduced to 3.76ng/g from 19.95ng/g.
AFB in embodiment 4,3382 pairs of peanut pastes of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 1.
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, and 25 ℃, shaking table rotation radius 20mm is after shaking speed is 180rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration, get an amount of fermented liquid according to count results, the centrifugal then yeast saccharomyces cerevisiae thalline that obtains, above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0) at 121 ℃ of heating 30min, and centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was finished.
Take by weighing the 2g peanut paste, detect through high performance liquid chromatography and obtain AFB in this peanut paste
1Concentration be 22.50 ± 1.31ng/g, this peanut paste is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 2.5 * 10
9Individual yeast cell/ml, 23 ℃ of adsorption temps are set, adsorption time 30min, shaking speed 200rpm, after effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the peanut paste of detoxification with the mixing solutions of above-mentioned thalline and peanut paste, detect AFB in the detoxification peanut paste through high performance liquid chromatography
1Concentration be 2.63 ± 0.30ng/g, this thalline is to AFB
1Adsorption rate reach 88.32 ± 1.31%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple mean values.
Reference examples 3
Take by weighing 2g peanut paste same as described above, detect and obtain AFB in this peanut paste
1Concentration be 22.50 ± 1.31ng/g, this peanut paste is added in the blank centrifuge tube, 23 ℃ of operative temperatures are set, action time 30min, shaking speed 200rpm, the effect finish after, with peanut paste 10, the centrifugal 10min of 000rpm collects upper solution, detects AFB in the peanut paste through high performance liquid chromatography
1Concentration be 2.63 ± 0.30ng/g.
Experimental result shows, 3382 pairs of peanut paste AFBs of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, thalline through 121 ℃ the heating 30min, 23 ℃ of adsorption temps, adsorption time 30min, when shaking speed was 200rpm, this thalline was to AFB in the peanut paste
1Adsorption rate reach 88.32 ± 1.31%, can make AFB in the peanut paste
1Concentration be reduced to 2.63 ± 0.30ng/g from 22.50 ± 1.31ng/g.
Claims (10)
1. yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1, its preservation registration number is CGMCC № 3382.
2. yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is at preparation adsorption of aflatoxin B
1Biotechnological formulation in application.
3. one kind prepares adsorption of aflatoxin B
1The method of biotechnological formulation, be ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382, the centrifugal supernatant liquor of removing of its fermented liquid is obtained thalline, be adsorption of aflatoxin B
1Biotechnological formulation.
4. method according to claim 3, it is characterized in that: the consisting of of the fermention medium of described ferment wine brewing yeast (Saccharomycescerevisiae) LYMT-Y1 CGMCC № .3382: yeast soaks powder 1%, peptone 2%, glucose 2%, all the other are water; Described percentage composition is the quality percentage composition; The pH value of described fermention medium is 7.0-7.2.
5. according to claim 3 or 4 described methods, it is characterized in that: described fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, rotation radius 20mm～26mm, shaking culture 30h～34h.
6. according to any described method among the claim 3-5, it is characterized in that: the thalline of described acquisition is also through 121 ℃ of heating.
7. method according to claim 6 is characterized in that: the step of described heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer again at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline.
8. the adsorption of aflatoxin B of any described method preparation among the claim 3-7
1Biotechnological formulation.
Among the claim 3-7 arbitrary described biotechnological formulation at adsorption of aflatoxin B
1In application.
10. application according to claim 9 is characterized in that: described adsorption of aflatoxin B
1Temperature condition be 20-30 ℃, be preferably 23 ℃; The time of described absorption is 30-120min, preferred 30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010109125XA CN101775358B (en) | 2010-02-08 | 2010-02-08 | Accharomyces cerevisiae and application thereof in adsorption of aflatoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010109125XA CN101775358B (en) | 2010-02-08 | 2010-02-08 | Accharomyces cerevisiae and application thereof in adsorption of aflatoxin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101775358A true CN101775358A (en) | 2010-07-14 |
| CN101775358B CN101775358B (en) | 2012-07-04 |
Family
ID=42511945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010109125XA Active CN101775358B (en) | 2010-02-08 | 2010-02-08 | Accharomyces cerevisiae and application thereof in adsorption of aflatoxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101775358B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727550A (en) * | 2012-07-10 | 2012-10-17 | 河北联合大学 | Antifungal artemisia argyi rhizome leavening and preparation technology thereof |
| CN107279686A (en) * | 2017-06-02 | 2017-10-24 | 中国农业科学院农产品加工研究所 | Application of the malicious aspergillus flavus in terms of aflatoxin degradation is not produced |
-
2010
- 2010-02-08 CN CN201010109125XA patent/CN101775358B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727550A (en) * | 2012-07-10 | 2012-10-17 | 河北联合大学 | Antifungal artemisia argyi rhizome leavening and preparation technology thereof |
| CN102727550B (en) * | 2012-07-10 | 2013-11-27 | 河北联合大学 | Antifungal artemisia argyi rhizome leavening and preparation technology thereof |
| CN107279686A (en) * | 2017-06-02 | 2017-10-24 | 中国农业科学院农产品加工研究所 | Application of the malicious aspergillus flavus in terms of aflatoxin degradation is not produced |
| CN107279686B (en) * | 2017-06-02 | 2019-11-08 | 中国农业科学院农产品加工研究所 | Application of the malicious aspergillus flavus in terms of aflatoxin degradation is not produced |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101775358B (en) | 2012-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11242502B2 (en) | Low urea-producing and flavor-producing Wickerhamomyces anomalus strain and use thereof in food production | |
| Zheng et al. | Complex microbiota of a Chinese “Fen” liquor fermentation starter (Fen-Daqu), revealed by culture-dependent and culture-independent methods | |
| Pandey | Recent process developments in solid-state fermentation | |
| Amoa‐Awua et al. | The contribution of moulds and yeasts to the fermentation of ‘agbelima’cassava dough | |
| CN103205374B (en) | Lactic acid bacterium capable of reducing aflatoxin B1 in fermented feed and application of lactic acid bacterium | |
| CN103205376B (en) | One strain is applicable to Lactobacillus plantarum and the application thereof of fermented feed | |
| Vera et al. | Description of a French natural wheat sourdough over 10 consecutive days focussing on the lactobacilli present in the microbiota | |
| Xiong et al. | PCR-DGGE Analysis of the Microbial Communities in Three Different Chinese | |
| CN104046569B (en) | Aspergillus tubingensis for high-yield production of glucoamylase, alpha-amylase and acidic protease and application thereof | |
| CN112662575A (en) | Saccharomycetes fibuligera with high protease activity and high wine yield, and composition and application thereof | |
| CN104673703A (en) | Bacillus capable of simultaneously promoting saccharomyces cerevisiae to produce ethyl alcohol and flavor substances and application of bacillus | |
| Morais et al. | Production of ethanol and xylanolytic enzymes by yeasts inhabiting rotting wood isolated in sugarcane bagasse hydrolysate | |
| CN110042072A (en) | A kind of aflatoxin degradation B1Bacterial strain and its application | |
| CN104371951A (en) | Serratia sp. A5 and uses thereof | |
| CN104630166A (en) | Method for producing low-temperature glucose oxidase by virtue of microbial fermentation | |
| CN110684691A (en) | Preparation process of microbial agent based on directional screening of microorganisms | |
| CN117343871B (en) | Acinetobacter baumannii, esterified liquid and application thereof in yellow water treatment | |
| CN101775358B (en) | Accharomyces cerevisiae and application thereof in adsorption of aflatoxin | |
| CN112342152B (en) | Lipase-expressing goat staphylococcus strain NCU S6 | |
| CN103045487A (en) | Bacterial strain for producing citric acid and method for fermenting and producing critic acid through fermentation of bacterial strain | |
| CN104250618B (en) | The aspergillus candidus of a kind of high-yield glucoamylase, alpha amylase and acid protease and its application | |
| Ogawa et al. | An original habitat of tempeh molds | |
| CN102643754B (en) | Aspergillus oryzae and application thereof in aspect of improving yield of alcohol | |
| CN101717789B (en) | Method for preparing culture medium for efficiently producing haematochrome | |
| Magalhães-Guedes et al. | Ethanol production from deproteinized cheese whey fermentations by co-cultures of Kluyveromyces marxianus and Saccharomyces cerevisiae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220126 Address after: Room 320, floor 3, building 49, yard 2, Yuanmingyuan West Road, Haidian District, Beijing 100193 Patentee after: Beijing Zhongnong taste detection technology Co.,Ltd. Address before: 100193 No. 2 Old Summer Palace West Road, Beijing, Haidian District Patentee before: INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES |