CN101773799B - Periodic peristaltic agitation method - Google Patents
Periodic peristaltic agitation method Download PDFInfo
- Publication number
- CN101773799B CN101773799B CN2010101136180A CN201010113618A CN101773799B CN 101773799 B CN101773799 B CN 101773799B CN 2010101136180 A CN2010101136180 A CN 2010101136180A CN 201010113618 A CN201010113618 A CN 201010113618A CN 101773799 B CN101773799 B CN 101773799B
- Authority
- CN
- China
- Prior art keywords
- periodic
- reactor drum
- materials
- resilient material
- agitation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a periodic peristaltic agitation method from the point of bionics and according to the principle of intestinal peristalsis and periodic stimulation effect. One or more devices generating periodic peristalsis is distributed in a reactor. The internal part of the device is isolated from materials in the reactor by using elastic materials. The elastic materials are controlled to be periodically expanded and contracted through the movement of the materials in the device. When the elastic materials are expanded, the points of the elastic materials in contact with the materials move along the direction of the materials to generate normal acting force which is perpendicular to the movement direction and is applied to the materials, so materials move along the direction of the normal acting force under the pushing effect of the acting force; and when the elastic materials are contracted, the movement direction of the materials is reverse. Thereby, with the periodic expansion and contraction of the materials in the device, the materials in the reactor alternately do peristaltic movement under the periodic normal acting force. The method strengths heat transfer and mass transfer through the normal acting force, is suitable for reactors for chemical industry, microorganism fermentation, cell culture and the like and can be industrially scaled.
Description
Technical field
The invention belongs to the stirring technique field, particularly a kind of periodic peristaltic agitation method.
Background technology
Stirring is a kind of with the uniform very effective reinforcement means of gas, liquid, solid mixing of materials, and alr mode can be divided into mechanical stirring and on-mechanical stirs.Traditional mechanical stirring is applied to every profession and trades such as chemical reaction, microbial fermentation, concrete mixing, main through strengthen between gas-liquid, interface such as liquid-solid relative movement with mix, utilize the momentum transfer means of tangent direction friction power to strengthen mass transfer, the heat transfer at interface.Mechanical stirring is in the application of every profession and trade, and according to concrete physical process and chemical process, churned mechanically form or type are had nothing in common with each other, and the influence that material is produced also has nothing in common with each other.For example; When mechanical stirring is applied to bio-reactor; Temperature, PH, dissolved oxygen and nutritive substance concentration in the reactor drum more do not have advantages such as more easy to control with the reactor drum of mechanical stirring device; But the shearing force that mechanical stirring causes causes bigger damage to organism, also can exert an influence to the synthetic of secondary metabolite.
The shearing action of considering mechanical stir-reactor is difficult to avoid, and therefore, in the culture plant cell reactor drum, develops and gas stirring formula on-mechanical stirred reactor.The reactor drum gas-liquid mass transfer of this type is effective, and the oxygen transfer efficiency is high, and shearing force is low, and is little to the damage of organism, realize long-term sterile culture easily, but turndown ratio is little, and especially when the late stage of culture cell density is higher, mixed effect is relatively poor during low gas velocity.If the raising air flow can produce significant foam again, microorganism growth there is inhibition.
And in the solid state fermentation bio-reactor; Machinery stirs the destruction to the mikrobe shearing force in the conventional solid-state fermentation in order to solve; The heat and mass transfer of enhanced reactor; The air pressure oscillation fermenter reactor drum has been invented by Chinese Academy Of Sciences Process Engineering Research Institute; It is that the gaseous pressure in the airtight low pressure vessel is imposed cycle pulsation, makes the mass transferring in gas phase in the fermentation bed of material become the forced convection diffusion by molecular diffusion, has solved solid state fermentation heat transfer, mass transfer difference and has been difficult to problem such as extensive purebred cultivation.Concrete main operation be sterile air to the punching press in the airtight low pressure vessel with rush down the existing periodically pulsing of compacting.
In the gas stirring formula culture plant cell reactor drum or air pressure oscillation fermenter reactor drum etc. alleviated traditional mechanical to a certain extent and stirred shearing force influence mikrobe, cell etc.; But the gas in the aforesaid way; Must be directly with reactor drum in material contact; In intensifying heat transfer, mass transfer, also increased the possibility of microbiological contamination.
Be for intensifying heat transfer and mass transfer equally; Reduce the shearing force influence of stirring to material; The Tong Jin of Jilin University, Hou Zhesheng etc. have developed a kind of fermentor tank (patent No. 200610017078.X) according to the bionic principle of peristole; It comes material is pushed, rubs through flexible wall and the inner E font support that the spring assembly of posting piezoelectric patches is fixedly connected, and realizes the stirring to solid substances.Though this fermentor tank can be realized the purpose of intensifying heat transfer, mass transfer to a certain extent, its internal structure is comparatively complicated, and material is in flexible wall inside, and volume is limited, and therefore difficulty is amplified in industry.
Living microbe has the susceptibility of environment to external world; Inherent regulation; Adaptive ability, it is a kind of to blanket optimization regulating measures such as bio-reaction systems that extraneous period stimulating is regulated, this period stimulating can be temperature, concentration, pressure, light, electricity, magnetic, sound etc.Li Zuohu; Chen Hongzhang etc. in bio-reactor, have designed above-mentioned pressure pulsation period stimulating solid-state fermentation reactor with the period stimulating principles, and pressure pulsation period stimulating reactor drum is reducing the mechanical stirring shearing force; When improving the material heat and mass transfer; The period stimulating effect is also lived etc. to protein in the fermentation process, key enzyme and is exerted an influence, and has promoted microbial growth, explains that period stimulating acts on to help microbial growth to a certain extent.
Summary of the invention
To present mechanical stirring mainly with shearing force reinforcing mass transfer, heat transfer; And on-mechanicals such as air-flow stirring stir only emphasical gas-liquid mass transfer, the direct contact with material of extraneous air-flow possibly cause microbiological contamination and conventional flexibility to rub the problems such as being difficult to industrial amplification that stirs, and the present invention is from the bionics angle, according to intestinal peristalsis and period stimulating action principle; A kind of periodic peristaltic agitation method has been proposed; Avoided the influence of shearing force to mikrobe, with periodic normal force intensifying heat transfer, mass transfer, the material in the reactor drum is in outside the wriggling whipping appts; Charge by wriggling whipping appts volume shrinkage limit system, is not convenient to industry and is amplified.
Its concrete technical scheme is:
The one or more devices that produce periodic peristaltic of distribution in reactor drum, this device can be sealing, also can be non-sealings.1 is resilient material in the device, and 2 for the device outside is a material in the reactor drum, and 3 is the material that the inner may command 1 of device expands or shrinks; Separate with resilient material 1 between device and the material, the material in 3 can along direction 4 or with 4 opposite direction periodicmotions, then resilient material 1 is periodic expands and shrinks; When resilient material 1 expands, the contacted point 5 of resilient material and material will move to the material direction, result from the vertical normal force 6 of heading and act on material; Material will move toward reactive force 6 identical directions, and when resilient material 1 shrank, then material movement was in the opposite direction; Therefore; Along with periodic expansion of the material in installing and contraction, the material in the reactor drum will alternately be wriggled under periodic normal force, thus the heat and mass transfer of material in the enhanced reactor.
Described device is for sealing or nonocclusive;
The expanding volume of described vermiculator maximum is 1: 10～1: 200 with the ratio of the volume of reactor drum;
Before described vermiculator expands with expand after volume ratio be 1: 2～1: 15;
The described wriggling cycle is 5min～120min;
Described reactor drum is chemical field reactor drum, microorganism solid fermentation reactor drum, liquid state fermentation reactor drum, culture plant cell reactor drum, animal cell culture reactor drum;
Described reactor drum is vertical or horizontal reactor;
Described device profile is rule or irregular tubulose, shape of threads, circle, ellipse;
Said resilient material is Zylox, latex, gel, ionic film polymer, conducting polymer, the flexible polymkeric substance of electronics;
Described resilient material is acid and alkali-resistance, nontoxic material;
Be that may command resilient material 1 expands and the material that shrinks in the described device 3;
1 expansion of may command resilient material is the liquid of gas, water, rigid objects, different PH, a kind of or arbitrary combination in the electroconductive with the material that shrinks in the described device 3.
Beneficial effect of the present invention is:
1, the invention provides and a kind ofly act on the periodicity peristaltic agitation method of material, avoided traditional mechanical stirring the influence, particularly shearing force of the shearing force of material shear fracture effect to active body in the bio-reactor with normal force.
2, the invention provides a kind of stirring means of periodicity wriggling, applicable to chemical field reactor drum, microorganism solid fermentation reactor drum, liquid state fermentation reactor drum, culture plant cell reactor drum, animal cell culture reactor drum etc.
3, the invention provides a kind of periodic peristaltic agitation method, strengthened the heat and mass transfer of material in the reactor drum, improve the reaction efficiency of reactor drum.
4, the invention provides a kind of stirring means of periodic peristaltic, the one-period hormesis is provided, help the carrying out or the microbial growth of chemical reaction the material in the reactor drum.
5, the stirring means of the periodic peristaltic of the present invention's proposition; Directly contact different with the gas that feeds in the stirring of gas stirring formula on-mechanical with material; With respect to the material in the reactor drum; Producing the device that wriggling stirs can seal, does not have exchange of substance with material in the reactor drum, has eliminated because other materials add the possibility of generation microbiological contaminations.
6, the device and the material in the reactor drum of generation periodic peristaltic can be non-sealings among the present invention, add the separation of realization products such as filtering membrane structure.
7, can add the one or more vermiculator stirring action of wriggling performance period in the reactor drum, simple in structure, the industry that helps reactor drum is amplified.
Description of drawings
Fig. 1 is the front view of periodic peristaltic alr mode generation device synoptic diagram;
Fig. 2 is the vertical view of periodic peristaltic alr mode generation device synoptic diagram;
Among the figure: material in 1 resilient material, 2 reactor drums, 3 is the material that the inner may command 1 of device expands or shrinks, the heading of material in 4 devices 1, the contacted point of 5 resilient materials and material, 6 normal force directions.
Embodiment
Through embodiment the present invention is further specified below.
Embodiment 1: peristaltic agitation method is applied to the starchy material solid state fermentation of ethanol through
In the horizontal solid-state fermentation reactor of a 50L, four vermiculators of wall uniform distribution on the limit, each device inside is the steel bead of non-uniform Distribution; Just the radius of ball is 5-15cm, and steel ball is fixed on the bar of movably wriggling, and resilient material is the latex material according to die production in the vermiculator; Material is the starchy material Ipomoea batatas of liquefying-saccharifying in the reactor drum; Behind the fermentation starting, open the wriggling rod driving device, the wriggling bar in the vermiculator is done opposite-oriented movement at interval; Wriggling bar movement velocity is 1m/s, and the period of motion is spaced apart 5min, makes the periodic expansion of latex material and shrinks, thereby impel the material in the reactor drum periodically to wriggle; After the fermentation ends, with traditional not comparing with the fermentation of vermiculator, fermentation time has shortened 6h, and starch utilization ratio improves 18.2%.
Embodiment 2: peristaltic agitation method is applied to culture plant cell
In the vertical reactor of a 25L, add two tubulose vermiculators, resilient material is the silica gel material according to die production in the vermiculator, the tubulose vermiculator is through the periodic air that feeds band pressure; Through regulating external air pressure, make the latex material of vermiculator expand into maximum volume 2.5L, under this state, in reactor drum, feed, add nutrient solution and nutritive salt etc., carry out paniculatum cell and cultivate; After the startup, the adjustment air pressure, making the air punching press of feeding vermiculator and the cycle of pressure release is 30min, punching press is held time and is 5min, makes the periodic expansion of silica gel material and shrinks, thereby impel the material in the reactor drum periodically to wriggle; After cultivating end, cell yield (dry-matter) can reach 10g/L.
Embodiment 3: peristaltic agitation method is applied to animal cell culture
In the vertical reactor of a 30L, add a shape of threads vermiculator, resilient material is the gelatinous material according to die production in the vermiculator, this gelatinous material can change the contraction of expanding according to liquid PH in the device; Through regulating the pH value of outside influent, make the latex material of vermiculator expand into maximum volume 1.5L, under this state, in reactor drum, feed, add nutrient solution and nutritive salt etc., carry out cell cultures; After the startup, the adjustment air pressure, making the air punching press that feeds vermiculator and the cycle of pressure release is 20min, punching press is held time and is carried out for 2min; Make the periodic expansion of gelatinous material and shrink, thereby impel the material in the reactor drum periodically to wriggle; After cultivate finishing, cell yield do not add vermiculator increase by 15%.
Embodiment 4: peristaltic agitation method is applied to citric acid fermentation
In the vertical liquid citric acid fermentation reactor drum of a 50L; Add the shape of threads vermiculator; Resilient material is the latex material according to die production in the vermiculator; Material is the starchy material cassava of liquefying-saccharifying in the reactor drum, and the tubulose vermiculator is through periodic 20-40 ℃ the water that feeds band pressure; Through regulating external water pressure, make the latex material of vermiculator expand into maximum volume 2.5L, under this state, in reactor drum, feed, insert citric acid fermentation bacterial classification and inorganic salt etc., start fermentation and carry out; Behind the fermentation starting, the adjustment water temperature, making the water temperature that feeds vermiculator is 25-35 ℃, and the cycle of punching press and pressure release is 30min, and punching press is held time and is 1min, makes the periodic expansion of latex material and shrinks, thereby impel the material in the reactor drum periodically to wriggle; After the fermentation ends, with traditional not comparing with the fermentation of vermiculator, fermentation time has shortened 6h.
Claims (7)
1. a periodic peristaltic agitation method is characterized in that, one or more devices that produce periodic peristaltic distribute in reactor drum; Device comprises resilient material (1), installs the material (3) that inner may command resilient material (1) expands or shrinks, and separates with resilient material (1) between the material (2) in device and the reactor drum; The material (3) that installs inner may command resilient material (1) expansion or shrink can be along the axial direction due periodicmotion of resilient material (1), and then resilient material (1) is periodic expands and contraction, when resilient material (1) expands; To move to the material direction with the contacted point of resilient material (1) (5); Produce with the vertical normal force of heading (6) and act on material, material will move toward the identical direction of normal force (6), and when resilient material (1) shrinks; Then material movement is in the opposite direction; Therefore, through periodic expansion of control resilient material (1) and contraction, the material in the reactor drum will alternately be wriggled under periodic normal force.
2. periodic peristaltic agitation method according to claim 1 is characterized in that: the expanding volume of described vermiculator maximum is 1: 2～1: 20 with the ratio of the volume of reactor drum.
3. periodic peristaltic agitation method according to claim 1 is characterized in that: before described vermiculator expands with expand after volume ratio be 1: 2～1: 15.
4. periodic peristaltic agitation method according to claim 1 is characterized in that: the described wriggling cycle is 5min-120min.
5. periodic peristaltic agitation method according to claim 1 is characterized in that: described stirring means is applicable to chemical field reactor drum, microorganism solid fermentation reactor drum, liquid state fermentation reactor drum, culture plant cell reactor drum, animal cell culture reactor drum.
6. periodic peristaltic agitation method according to claim 1 is characterized in that: the flexible polymkeric substance of gel, ionic film polymer, conducting polymer, electronics that said resilient material is acid and alkali-resistance, nontoxic Zylox, latex, change with pH value.
7. periodic peristaltic agitation method according to claim 1 is characterized in that: in the described device may command resilient material (1) expand with the material (3) that shrinks be the liquid of gas, rigid objects, different PH, one or both or arbitrary combination in the electroconductive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101136180A CN101773799B (en) | 2010-02-24 | 2010-02-24 | Periodic peristaltic agitation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101136180A CN101773799B (en) | 2010-02-24 | 2010-02-24 | Periodic peristaltic agitation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101773799A CN101773799A (en) | 2010-07-14 |
| CN101773799B true CN101773799B (en) | 2012-11-28 |
Family
ID=42510465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101136180A Expired - Fee Related CN101773799B (en) | 2010-02-24 | 2010-02-24 | Periodic peristaltic agitation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101773799B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105457545B (en) * | 2015-12-25 | 2024-06-11 | 重庆欣欣向荣精细化工有限公司 | Lifting magnetic suspension stirring device |
| CN108889085B (en) * | 2018-08-07 | 2021-01-12 | 内蒙古美方煤焦化有限公司 | Coking flue gas denitration device |
| CN111014241B (en) * | 2019-12-13 | 2021-06-25 | 湖州聚业孵化器有限公司 | Industry collection system that gives up admittedly |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009895A1 (en) * | 1992-11-02 | 1994-05-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Reactor system |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
-
2010
- 2010-02-24 CN CN2010101136180A patent/CN101773799B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009895A1 (en) * | 1992-11-02 | 1994-05-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Reactor system |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
Non-Patent Citations (2)
| Title |
|---|
| 侯哲生，佟金.蠕动发酵罐的仿生耦合设计.《农业机械学报》.2007,第38卷(第6期), * |
| 陈洪章,李佐虎,陈继贞.周期刺激固态发酵生产纤维素酶.《精细与专用化学品》.1999,(第21期),第37-39页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101773799A (en) | 2010-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Influence of mixing method and hydraulic retention time on hydrogen production through photo-fermentation with mixed strains | |
| CN101773799B (en) | Periodic peristaltic agitation method | |
| Fahim et al. | Impact of energy supply and oxygen transfer on selective lipopeptide production by Bacillus subtilis BBG21 | |
| Cheng et al. | Strengthening mass transfer of carbon dioxide microbubbles dissolver in a horizontal tubular photo-bioreactor for improving microalgae growth | |
| JP2007312689A (en) | Method for performing biological reaction, and device for performing the biological reaction | |
| US9222108B2 (en) | Bioreactor process for production of hydrogen from biomass | |
| CN107815446B (en) | A kind of fermentation process in high density of recombination nitrile hydratase Recombinant organism | |
| CN109136082A (en) | A kind of membrane bioreactor and helotism system of microalgae and methane-oxidizing bacteria symbiosis reinforcing methane producing bio-oil | |
| Margaritis et al. | Novel bioreactor systems and their applications | |
| CN103103130B (en) | Production method for lipid through mixed culture of microbes | |
| CN112111393A (en) | Bionic intestinal flow self-peristaltic reactor system and method | |
| CN102643770A (en) | Escherichia coli for producing succinic acid by utilizing pure anaerobic growth of synthetic culture medium and application thereof | |
| CN217459384U (en) | Oxygen micro-nano bubble enhanced aerobic fermentation bioreactor | |
| CN201686699U (en) | Rotary solid-state fermentation bioreactor | |
| CN104911218B (en) | A kind of method for improving the high Produced by Solid-state Fermentation biogas of kitchen garbage | |
| CN109053867A (en) | A kind of cultural method of high density fermentation expression recombinant rotavirus Δ VP8* antigen | |
| CN102127505A (en) | Immobilized cell bioreactor | |
| CN203999629U (en) | Magnetic motive force stirred fermentor | |
| CN106636238A (en) | Method for producing gamma-amino butyric acid through distillers' grains fermentation by microorganisms | |
| CN102925355B (en) | Wave generation type cell bioreactor | |
| WO2012152337A1 (en) | Segmented flow biofilm reactor | |
| CN110042046A (en) | Continuous immobilized internal stirring micro-membrane aeration internal circulation airlift bioreactor | |
| CN108728389A (en) | One plant of colibacillus engineering and its application for producing 2,3,5,6- Tetramethylpyrazines | |
| CN106834264A (en) | A kind of use for laboratory bacteria adhension method | |
| CN101044824B (en) | Culturing apparatus for improving growth of hair root of medicinal plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20160224 |