CN101773799A - Periodic peristaltic agitation method - Google Patents
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- CN101773799A CN101773799A CN 201010113618 CN201010113618A CN101773799A CN 101773799 A CN101773799 A CN 101773799A CN 201010113618 CN201010113618 CN 201010113618 CN 201010113618 A CN201010113618 A CN 201010113618A CN 101773799 A CN101773799 A CN 101773799A
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Abstract
The invention discloses a periodic peristaltic agitation method from the point of bionics and according to the principle of intestinal peristalsis and periodic stimulation effect. One or more devices generating periodic peristalsis is distributed in a reactor. The internal part of the device is isolated from materials in the reactor by using elastic materials. The elastic materials are controlled to be periodically expanded and contracted through the movement of the materials in the device. When the elastic materials are expanded, the points of the elastic materials in contact with the materials move along the direction of the materials to generate normal acting force which is perpendicular to the movement direction and is applied to the materials, so materials move along the direction of the normal acting force under the pushing effect of the acting force; and when the elastic materials are contracted, the movement direction of the materials is reverse. Thereby, with the periodic expansion and contraction of the materials in the device, the materials in the reactor alternately do peristaltic movement under the periodic normal acting force. The method strengths heat transfer and mass transfer through the normal acting force, is suitable for reactors for chemical industry, microorganism fermentation, cell culture and the like and can be industrially scaled.
Description
Technical field
The invention belongs to the stirring technique field, particularly a kind of periodic peristaltic agitation method.
Background technology
Stirring is a kind of with the uniform very effective reinforcement means of gas, liquid, solid mixing of materials, and alr mode can be divided into mechanical agitation and on-mechanical stirs.Traditional mechanical agitation is applied to every profession and trades such as chemical reaction, microbial fermentation, concrete mixing, main by strengthen between gas-liquid, interface such as liquid-solid relative motion with mix, utilize the Momentum Transfer means of tangent direction friction power to strengthen mass transfer, the heat transfer at interface.Mechanical agitation is in the application of every profession and trade, and according to concrete physical process and chemical process, churned mechanically form or type are had nothing in common with each other, and the influence that material is produced also has nothing in common with each other.For example, when mechanical agitation is applied to bioreactor, temperature, PH, dissolved oxygen and nutriment concentration in the reactor more do not have advantages such as more easy to control with the reactor of mechanical stirring device, but the shearing force that mechanical agitation causes causes bigger damage to organism, also can exert an influence to the synthetic of secondary metabolite.
The shear action of considering mechanical stir-reactor is difficult to avoid, and therefore, in the culture plant cell reactor, develops and gas stirring-type on-mechanical stirred reactor.The reactor gas-liquid mass transfer of this type is effective, oxygen transmission efficiency height, and shearing force is low, and is little to the damage of organism, realize long-term Aseptic Culture easily, but operating flexibility is little, and especially when the late stage of culture cell density is higher, mixed effect is relatively poor during low gas velocity.If the raising throughput can produce a large amount of foams again, growth of microorganism there is inhibition.
And in the solid state fermentation bioreactor, in order to solve the mechanical destruction of stirring the microorganism shearing force in the conventional solid-state fermentation, the heat and mass transfer of enhanced reactor, the air pressure oscillation fermenter reactor has been invented by Chinese Academy Of Sciences Process Engineering Research Institute, it is that the gaseous pressure in the airtight low pressure vessel is imposed the cycle pulsation, make the mass transferring in gas phase of fermentation in the bed of material become the forced convertion diffusion, solved solid state fermentation heat transfer, mass transfer difference and be difficult to problem such as extensive purebred cultivation by molecular diffusion.Concrete main operation be filtrated air to the punching press in the airtight low pressure vessel with rush down the existing periodically pulsing of compacting.
In the gas stirring-type culture plant cell reactor or air pressure oscillation fermenter reactor etc. alleviated of the shearing force influence of traditional mechanical agitation to a certain extent to microorganism, cell etc., but the gas in the aforesaid way, must be directly contact with material in the reactor, in augmentation of heat transfer, mass transfer, also increased the possibility of microbiological contamination.
Be for augmentation of heat transfer and mass transfer equally, reduce the shearing force influence of stirring to material, the Tong Jin of Jilin University, Hou Zhesheng etc. have developed a kind of fermentation tank (patent No. 200610017078.X) according to the bionic principle of peristole, it comes material is pushed, rubs by the groups of springs of posting piezoelectric patches fixedly connected flexible wall and inner E font support, realizes the stirring to solid material.Though this fermentation tank can be realized the purpose of augmentation of heat transfer, mass transfer to a certain extent, its internal structure is comparatively complicated, and material is in flexible wall inside, and volume is limited, and therefore difficulty is amplified in industry.
Biological living has the sensitiveness of environment to external world, self-regulation, adaptive ability, it is a kind of to blanket optimization regulating measures such as bio-reaction systems that extraneous period stimulating is regulated, this period stimulating can be temperature, concentration, pressure, light, electricity, magnetic, sound etc.Li Zuohu, Chen Hongzhang etc. apply to bioreactor with the period stimulating principle, designed above-mentioned pressure fluctuation period stimulating solid-state fermentation reactor, pressure fluctuation period stimulating reactor is reducing the mechanical agitation shearing force, when improving the material heat and mass transfer, the period stimulating effect is also lived etc. to protein in the fermentation process, key enzyme and is exerted an influence, and has promoted microbial growth, illustrates that period stimulating acts on to help microbial growth to a certain extent.
Summary of the invention
Mainly strengthen mass transfer at present mechanical agitation with shearing force, conduct heat, and gas-liquid mass transfer is only emphasized in the stirring of on-mechanicals such as air-flow stirring, extraneous air-flow directly contacts with material and may cause microbiological contamination and conventional flexibility to rub the problems such as being difficult to industrial amplification that stirs, the present invention is from the bionics angle, according to enterocinesia and period stimulating action principle, a kind of periodic peristaltic agitation method has been proposed, avoided the influence of shearing force to microorganism, with periodic normal force augmentation of heat transfer, mass transfer, material in the reactor is in outside the wriggling agitating device, charge by wriggling agitating device volume shrinkage limit system, is not convenient to industry and is amplified.
Its concrete technical scheme is:
The one or more devices that produce periodic peristaltic of distribution in reactor, this device can be sealing, also can be non-sealings.1 is elastomeric material in the device, 2 for the device outside be material in the reactor, 3 for controlling 1 material that expands or shrink in device inside, separate with elastomeric material 1 between device and the material, material in 3 can be along direction 4 or the direction periodic motion opposite with 4, then elastomeric material 1 periodic expansion and contraction, when elastomeric material 1 expands, the contacted point 5 of elastomeric material and material will move to the material direction, result from the vertical normal force of the direction of motion 6 and act on material, material will move toward active force 6 identical directions, and when elastomeric material 1 shrank, then the direction of material movement was opposite, therefore, along with periodic expansion of the material in installing and contraction, the material in the reactor will alternately be wriggled under periodic normal force, thus the heat and mass transfer of material in the enhanced reactor.
Described device is for sealing or nonocclusive;
The expanding volume of described vermiculator maximum is 1: 10～1: 200 with the ratio of the volume of reactor;
Before described vermiculator expands with expand after volume ratio be 1: 2～1: 15;
The described wriggling cycle is 5min～120min;
Described reactor is chemical field reactor, microorganism solid fermentation reactor, liquid state fermentation reactor, culture plant cell reactor, animal cell culture reactor;
Described reactor is vertical or horizontal reactor;
Described device profile is rule or irregular tubulose, screw-like, circle, ellipse;
Described elastomeric material is silicon rubber, latex, gel, ionic film polymer, conducting polymer, the flexible polymer of electronics;
Described elastomeric material is acid and alkali-resistance, nontoxic material;
Expand and the material that shrinks for controlling elastomeric material 1 in the described device 3;
Can control elastomeric material 1 expansion in the described device 3 is the liquid of gas, water, rigid objects, different PH, a kind of or any combination in the electric conductor with the material that shrinks.
Beneficial effect of the present invention is:
1, the invention provides and a kind ofly act on the periodicity peristaltic agitation method of material, avoided traditional mechanical agitation the influence, particularly shearing force of the shearing force of material failure by shear effect to active body in the bioreactor with normal force.
2, the invention provides a kind of stirring means of periodicity wriggling, applicable to chemical field reactor, microorganism solid fermentation reactor, liquid state fermentation reactor, culture plant cell reactor, animal cell culture reactor etc.
3, the invention provides a kind of periodic peristaltic agitation method, strengthened the heat and mass transfer of material in the reactor, improve the reaction efficiency of reactor.
4, the invention provides a kind of stirring means of periodic peristaltic, provide the one-period spread effect, help the carrying out or the microbial growth of chemical reaction the material in the reactor.
5, the stirring means of the periodic peristaltic of the present invention's proposition, directly contact different with the gas that feeds in the stirring of gas stirring-type on-mechanical with material, with respect to the material in the reactor, the device that produces the wriggling stirring can seal, do not have mass exchange with the material in the reactor, eliminated because other materials add the possibility that microbiological contamination takes place.
6, the device and the material in the reactor of generation periodic peristaltic can be non-sealings among the present invention, add the separation of realization products such as filter membrane structure.
7, can add the one or more vermiculator stirring action of wriggling performance period in the reactor, simple in structure, the industry that helps reactor is amplified.
Description of drawings
Fig. 1 is the front view of periodic peristaltic alr mode generation device schematic diagram;
Fig. 2 is the vertical view of periodic peristaltic alr mode generation device schematic diagram;
Among the figure: material in 1 elastomeric material, 2 reactors, 3 for controlling 1 material that expands or shrink in device inside, the direction of motion of material in 4 devices 1, the contacted point of 5 elastomeric materials and material, 6 normal force directions.
The specific embodiment
The present invention will be further described below by embodiment.
Embodiment 1: peristaltic agitation method is applied to the starchy material solid state fermentation of ethanol through
In the horizontal solid-state fermentation reactor of a 50L, at even four vermiculators that distribute of limit wall, each device inside is the steel bead of non-uniform Distribution, just the radius of ball is 5-15cm, and steel ball is fixed on the bar of movably wriggling, and elastomeric material is the latex material of producing according to mould in the vermiculator, material is the starchy material Ipomoea batatas of liquefying-saccharifying in the reactor, behind the fermentation starting, open the wriggling rod driving device, the wriggling bar in the vermiculator is done the rightabout motion at interval; Wriggling bar movement velocity is 1m/s, and the period of motion is spaced apart 5min, makes the periodic expansion of latex material and shrinks, thereby impel the material in the reactor periodically to wriggle; After the fermentation ends, with traditional not comparing with the fermentation of vermiculator, fermentation time has shortened 6h, and starch utilization ratio improves 18.2%.
Embodiment 2: peristaltic agitation method is applied to culture plant cell
In the vertical reactor of a 25L, add two tubulose vermiculators, elastomeric material is the silica gel material of producing according to mould in the vermiculator, the tubulose vermiculator is by the periodic air that feeds band pressure; By regulating external air pressure, make the latex material of vermiculator expand into maximum volume 2.5L, under this state, in reactor, feed, add nutrient solution and nutritive salt etc., carry out paniculatum cell and cultivate; After the startup, adjust air pressure, making the air punching press of feeding vermiculator and the cycle of pressure release is 30min, and punching press is held time and is 5min, makes the periodic expansion of silica gel material and shrinks, thereby impel the material in the reactor periodically to wriggle; After cultivating end, cell yield (dry) can reach 10g/L.
Embodiment 3: peristaltic agitation method is applied to animal cell culture
In the vertical reactor of a 30L, add a screw-like vermiculator, elastomeric material is the gel rubber material of producing according to mould in the vermiculator, this gel rubber material can change the contraction of expanding according to liquid PH in the device; By regulating the pH value of outside influent, make the latex material of vermiculator expand into maximum volume 1.5L, under this state, in reactor, feed, add nutrient solution and nutritive salt etc., carry out cell and cultivate; After the startup, adjust air pressure, making the air punching press that feeds vermiculator and the cycle of pressure release is 20min, and punching press is held time and carried out for 2min; Make the periodic expansion of gel rubber material and shrink, thereby impel the material in the reactor periodically to wriggle; After cultivate finishing, cell yield do not add vermiculator increase by 15%.
Embodiment 4: peristaltic agitation method is applied to citric acid fermentation
In the vertical liquid citric acid fermentation reactor of a 50L, add the screw-like vermiculator, elastomeric material is the latex material of producing according to mould in the vermiculator, material is the starchy material cassava of liquefying-saccharifying in the reactor, and the tubulose vermiculator is by periodic 20-40 ℃ the water that feeds band pressure; By regulating external water pressure, make the latex material of vermiculator expand into maximum volume 2.5L, under this state, in reactor, feed, insert citric acid fermentation bacterial classification and inorganic salts etc., start fermentation and carry out; Behind the fermentation starting, adjust water temperature, making the water temperature that feeds vermiculator is 25-35 ℃, and the cycle of punching press and pressure release is 30min, and punching press is held time and is 1min, makes the periodic expansion of latex material and shrinks, thereby impel the material in the reactor periodically to wriggle; After the fermentation ends, with traditional not comparing with the fermentation of vermiculator, fermentation time has shortened 6h.
Claims (7)
1. a periodic peristaltic agitation method is characterized in that, one or more devices that produce periodic peristaltic distribute in reactor; 1 is elastomeric material in the device, 2 is material in the reactor, 3 for controlling 1 material that expands or shrink in device inside, separate with elastomeric material 1 between device and the material, material in 3 can be along direction 4 or the direction periodic motion opposite with 4, then elastomeric material 1 periodic expansion and contraction, when elastomeric material 1 expands, to move to the material direction with 1 contacted point 5, produce the normal force 6 vertical and act on material with the direction of motion, material will move toward active force 6 identical directions, and when elastomeric material 1 shrank, then the direction of material movement was opposite, therefore, by the 1 periodic expansion and contraction of control elastomeric material, the material in the reactor will alternately be wriggled under periodic normal force.
2. periodic peristaltic agitation method according to claim 1 is characterized in that: the expanding volume of described vermiculator maximum is 1: 2～1: 200 with the ratio of the volume of reactor.
3. periodic peristaltic agitation method according to claim 1 is characterized in that: before described vermiculator expands with expand after volume ratio be 1: 2～1: 500.
4. periodic peristaltic agitation method according to claim 1 is characterized in that: the described wriggling cycle is 5s～24h.
5. periodic peristaltic agitation method according to claim 1 is characterized in that: described stirring means is applicable to chemical field reactor, microorganism solid fermentation reactor, liquid state fermentation reactor, culture plant cell reactor, animal cell culture reactor.
6. periodic peristaltic agitation method according to claim 1 is characterized in that: the flexible polymer of gel, ionic film polymer, conducting polymer, electronics that described elastomeric material is acid and alkali-resistance, nontoxic silicon rubber, latex, change with pH value.
7. periodic peristaltic agitation method according to claim 1 is characterized in that: can control in the described device 3 elastomeric material 1 expand with the materials that shrink be the liquid of gas, water, rigid objects, different PH, one or both or combination arbitrarily in the electric conductor.
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| CN2010101136180A CN101773799B (en) | 2010-02-24 | 2010-02-24 | Periodic peristaltic agitation method |
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| CN101773799A true CN101773799A (en) | 2010-07-14 |
| CN101773799B CN101773799B (en) | 2012-11-28 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105457545A (en) * | 2015-12-25 | 2016-04-06 | 重庆欣欣向荣精细化工有限公司 | Lift type magnetic suspension stirring device |
| CN108889085A (en) * | 2018-08-07 | 2018-11-27 | 石宏扬 | A kind of coking equipment for denitrifying flue gas |
| CN111014241A (en) * | 2019-12-13 | 2020-04-17 | 湖州三新汽车环保科技有限公司 | Industry collection system that gives up admittedly |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009895A1 (en) * | 1992-11-02 | 1994-05-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Reactor system |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
-
2010
- 2010-02-24 CN CN2010101136180A patent/CN101773799B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009895A1 (en) * | 1992-11-02 | 1994-05-11 | Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno | Reactor system |
| CN1904031A (en) * | 2006-08-08 | 2007-01-31 | 吉林大学 | Fermentation tank |
Non-Patent Citations (2)
| Title |
|---|
| 《农业机械学报》 20070630 侯哲生，佟金 蠕动发酵罐的仿生耦合设计 第38卷, 第6期 * |
| 《精细与专用化学品》 19991115 陈洪章,李佐虎,陈继贞 周期刺激固态发酵生产纤维素酶 第37-39页 1-7 , 第21期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105457545A (en) * | 2015-12-25 | 2016-04-06 | 重庆欣欣向荣精细化工有限公司 | Lift type magnetic suspension stirring device |
| CN108889085A (en) * | 2018-08-07 | 2018-11-27 | 石宏扬 | A kind of coking equipment for denitrifying flue gas |
| CN111014241A (en) * | 2019-12-13 | 2020-04-17 | 湖州三新汽车环保科技有限公司 | Industry collection system that gives up admittedly |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
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| CN101773799B (en) | 2012-11-28 |
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