CN101773476A - Preparation method for laver antihypertensive peptide particles - Google Patents
Preparation method for laver antihypertensive peptide particles Download PDFInfo
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- CN101773476A CN101773476A CN201010116584A CN201010116584A CN101773476A CN 101773476 A CN101773476 A CN 101773476A CN 201010116584 A CN201010116584 A CN 201010116584A CN 201010116584 A CN201010116584 A CN 201010116584A CN 101773476 A CN101773476 A CN 101773476A
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- laver
- enzymolysis
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- antihypertensive peptide
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Abstract
The invention relates to a preparation method for laver antihypertensive peptide particles, which comprises materials in percent by weight and comprises the following operation steps that: (1) laver is taken as raw material, is dried and is crushed and AS.1398 neutral protease is used for enzymolysising dry laver powder in a constant-temperature water bath kettle; the enzymolysis conditions are that: water is added into dry laver powder, the ratio of material to liquid is 1:20, the enzymolysis temperature is 50 DEG C, the enzymolysis pH value is 7.5, the enzyme dosage is 10000U per gram dry laver powder and the enzymolysis time is 10 hours; (2) after the enzymolysis is completed, the enzymolysis liquid is inactivated in water bath with 100 DEG C for 10 minutes; (3) after the sediment in the enzymolysis liquid is removed through centrifugal process, the supernatant fluid is separated through ultrafiltration and the filtered liquid is sprayed and dried in a spray dryer to obtain laver polypeptide powder; and (4) the laver polypeptide powder is evenly mixed with maltodextrin according to the ratio of 1: 2, 1.5 percent of asugryn is added and 95 percent of edible alcohol is added to regulate humidity to prepare laver antihypertensive peptide particles. If the laver antihypertensive peptide particles are taken for long time, good efficacy on diseases such as high blood pressure is obtained.
Description
Technical field
The present invention relates to a kind of preparation method of laver antihypertensive peptide particles.
Background technology
At present, treat hypertensive medicine and generally adopt chemical synthetic drug, but the hyperpietic takes chemical synthetic drug for a long time, can produce various side effects, medical worker and patient more favor in synthetic drug therapy non-chemically.The blood pressure lowering peptide is called inhibiting peptide of tonin again, is the biologically active peptide with hypotensive activity, wherein, food endogenous binding protein blood pressure lowering peptide because it is nontoxic, have no side effect, safe, and the normotensive do not had hypotensive effect, be fit to long-term edible.Protein content is higher in the Thallus Porphyrae, and contains a large amount of vitamin, aminoacid, mineral, trace element and bioactive substance and medicinal ingredient, can be used for eating the making of endogenous binding protein blood pressure lowering peptide.
Summary of the invention
Purpose of the present invention is the preparation method that will propose a kind of laver antihypertensive peptide particles, Thallus Porphyrae is carried out enzymolysis after, obtain laver polypeptide through ultra-filtration and separation purification, spray drying.With laver polypeptide and dextrin, cyclamate mixed pelletization, make laver antihypertensive peptide particles.Laver antihypertensive peptide particles is quickly dissolving in boiling water, safe for drinking.
The present invention is achieved in that the preparation method of described a kind of laver antihypertensive peptide particles, and its operating procedure is as follows, percentage by weight,
(1) Thallus Porphyrae is a raw material, and pulverize the oven dry back, carries out enzymolysis with the AS.1398 neutral protease in thermostat water bath; Enzymatic hydrolysis condition is, Thallus Porphyrae dry powder adds water, and solid-liquid ratio is 1: 20, and 50 ℃ of hydrolysis temperatures, enzymolysis pH value 7.5, enzyme concentration are that every gram Thallus Porphyrae dry powder adds 10000U, enzymolysis time 10 hours;
(2) after enzymolysis finished, enzymolysis solution was in 100 ℃ of water-baths deactivation in 10 minutes;
(3) with the centrifugal removal post precipitation of enzymolysis solution, supernatant is through ultra-filtration and separation, and filter liquor is spray drying in spray dryer, obtains the laver polypeptide powder;
(4) laver polypeptide powder and maltodextrin are carried out mixing with 1: 2 ratio, add 1.5% sweet close element, add 95% edible ethanol and be adjusted to the particulate humidity of suitable system, mixing in blender, and cross 80 mesh sieves and granulate, dried 1 hour for 60 ℃, get laver antihypertensive peptide particles.
The invention has the beneficial effects as follows that obtain to have the polypeptide of buck functionality by the enzymolysis Thallus Porphyrae, and prepare the laver antihypertensive peptide particles with instant effect, long-term drink has good effect to diseases such as prophylaxis of hypertension.
The specific embodiment
The preparation method of a kind of laver antihypertensive peptide particles of the present invention is selected porphyra haitanensis for use, and kept dry is rubbed in porphyra haitanensis oven dry back in pulverizer.Take by weighing a certain amount of porphyra haitanensis dry powder, press 20 extraordinarily entry of dry powder weight, furnishing homogenate adds the AS.1398 neutral protease enzymolysis, and enzyme concentration is that the every gram of porphyra haitanensis dry powder adds 10000U, 50 ℃ of hydrolysis temperatures, enzymolysis pH value 7.5, enzymolysis time 10 hours; After finishing, enzymolysis makes the protease deactivation, in 8000rmin at 100 ℃ of following heating in water bath 10min
-1Centrifugal 15min collects supernatant.Supernatant is the microporous filter membrane microfiltration of 10 μ m through the aperture, tentatively remove large granular impurity, with clarifying enzymolysis solution is the ultrafilter membrane of 2000Da by molecular cut off, obtain the filter liquor of polypeptide molecular weight less than 2000Da, with filter liquor spray drying in spray dryer, obtain the laver polypeptide powder again; Laver polypeptide powder and maltodextrin are carried out mixing with 1: 2 ratio, add 1.5% sweet close element, add 95% edible ethanol and be adjusted to the particulate humidity of suitable system, mixing in blender, and cross 80 mesh sieves and granulate, dried 1 hour for 60 ℃, get laver antihypertensive peptide particles.
The present invention is that raw material, preparation technology are simple, the product chemical constitution is simple with natural Thallus Porphyrae, and not only antihypertensive effect is remarkable, and does not influence normal arterial pressure, is fit to take for a long time.Therefore, laver antihypertensive peptide particles is a kind of antihypertensive drugs and functional food with potentiality to be exploited.
Claims (3)
1. the preparation method of a laver antihypertensive peptide particles, its operating procedure is as follows, percentage by weight,
(1) Thallus Porphyrae is a raw material, and pulverize the oven dry back, carries out enzymolysis with the AS.1398 neutral protease in thermostat water bath; Enzymatic hydrolysis condition is: Thallus Porphyrae dry powder adds water, and solid-liquid ratio is 1: 20, and 50 ℃ of hydrolysis temperatures, enzymolysis pH value 7.5, enzyme concentration are that every gram Thallus Porphyrae dry powder adds 10000U, enzymolysis time 10 hours;
(2) after enzymolysis finished, enzymolysis solution was in 100 ℃ of water-baths deactivation in 10 minutes;
(3) with the centrifugal removal post precipitation of enzymolysis solution, supernatant is through ultra-filtration and separation, and filter liquor is spray drying in spray dryer, obtains the laver polypeptide powder;
(4) laver polypeptide powder and maltodextrin are carried out mixing with 1: 2 ratio, add 1.5% sweet close element, add 95% edible ethanol and be adjusted to the particulate humidity of suitable system, make granule, make laver antihypertensive peptide particles.
2. according to the preparation method of the described a kind of laver antihypertensive peptide particles of claim 1, it is characterized in that: the described ultra-filtration and separation of step 3, the ultrafilter membrane specification of selecting for use in its separation process is molecular cut off 2000Da.
3. according to the preparation method of the described a kind of laver antihypertensive peptide particles of claim 1, it is characterized in that: step 4 is made the granule process and is, mixing in blender, and cross 80 mesh sieves and granulate, dried 1 hour for 60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010116584A CN101773476A (en) | 2010-02-24 | 2010-02-24 | Preparation method for laver antihypertensive peptide particles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010116584A CN101773476A (en) | 2010-02-24 | 2010-02-24 | Preparation method for laver antihypertensive peptide particles |
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| CN101773476A true CN101773476A (en) | 2010-07-14 |
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| CN201010116584A Pending CN101773476A (en) | 2010-02-24 | 2010-02-24 | Preparation method for laver antihypertensive peptide particles |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
| CN102276687A (en) * | 2011-06-29 | 2011-12-14 | 王建忠 | Method for separating golden vinegar polypeptide extracts from golden vinegar and product thereof |
| CN102495169A (en) * | 2011-11-16 | 2012-06-13 | 江南大学 | Purifying and analyzing identification method for anti-oxidative peptide after controlled-enzymatic hydrolysis of laver |
| CN106819960A (en) * | 2017-01-15 | 2017-06-13 | 哈尔滨伟平科技开发有限公司 | The preparation method of laver powder |
-
2010
- 2010-02-24 CN CN201010116584A patent/CN101773476A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
| CN102154423B (en) * | 2011-01-12 | 2013-03-13 | 淮海工学院 | Method for preparing active peptide of laver |
| CN102276687A (en) * | 2011-06-29 | 2011-12-14 | 王建忠 | Method for separating golden vinegar polypeptide extracts from golden vinegar and product thereof |
| CN102276687B (en) * | 2011-06-29 | 2014-05-14 | 王建忠 | Method for separating golden vinegar polypeptide extracts from golden vinegar and product thereof |
| CN102495169A (en) * | 2011-11-16 | 2012-06-13 | 江南大学 | Purifying and analyzing identification method for anti-oxidative peptide after controlled-enzymatic hydrolysis of laver |
| CN106819960A (en) * | 2017-01-15 | 2017-06-13 | 哈尔滨伟平科技开发有限公司 | The preparation method of laver powder |
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Open date: 20100714 |