CN101773260B - Method for producing collagen-stabilized instant sea cucumbers - Google Patents
Method for producing collagen-stabilized instant sea cucumbers Download PDFInfo
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- CN101773260B CN101773260B CN200910016953.6A CN200910016953A CN101773260B CN 101773260 B CN101773260 B CN 101773260B CN 200910016953 A CN200910016953 A CN 200910016953A CN 101773260 B CN101773260 B CN 101773260B
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- sea cucumber
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- nitrogen
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 43
- 238000004519 manufacturing process Methods 0.000 title abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000004806 packaging method and process Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 9
- 229920001661 Chitosan Polymers 0.000 claims abstract description 6
- 230000007935 neutral effect Effects 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 5
- 108060008539 Transglutaminase Proteins 0.000 claims abstract description 4
- 235000011194 food seasoning agent Nutrition 0.000 claims abstract description 4
- 230000036541 health Effects 0.000 claims abstract description 4
- 238000005470 impregnation Methods 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 102000003601 transglutaminase Human genes 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 241000965254 Apostichopus japonicus Species 0.000 claims description 8
- 239000007789 gas Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 3
- 238000006073 displacement reaction Methods 0.000 claims description 3
- 230000005674 electromagnetic induction Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 229920006255 plastic film Polymers 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000006467 substitution reaction Methods 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 claims description 2
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 2
- 239000004223 monosodium glutamate Substances 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000000796 flavoring agent Substances 0.000 abstract description 5
- 235000019634 flavors Nutrition 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000000975 bioactive effect Effects 0.000 abstract description 4
- 239000004615 ingredient Substances 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 102000008186 Collagen Human genes 0.000 abstract description 2
- 108010035532 Collagen Proteins 0.000 abstract description 2
- 229920001436 collagen Polymers 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 2
- 238000006424 Flood reaction Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 101710123874 Protein-glutamine gamma-glutamyltransferase Proteins 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 210000002816 gill Anatomy 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 241000258955 Echinodermata Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Abstract
The invention discloses a method for producing collagen-stabilized instant sea cucumbers, which includes the following steps: (a) disemboweling, washing and precooking of sea cucumbers; (b) vacuum impregnation in neutral water-soluble chitosan solution and curing in transglutaminase solution; (c) seasoning; (d) vacuum nitrogen-filled packaging; (e) high-pressure steam sterilization. The produced sea cucumber has the following two characteristics: (1) besides the original nutrition and bioactive ingredients, the sea cucumber also has good sea cucumber flavor and tissue structure, that is, the sea cucumber is delicious food and can protect health; (2) since the collagen is stable, the storage period is long, one year under the condition of 0 DEG C to 4 DEG C and three months under the condition of room temperature. Consequently, the sea cucumber is highly popular with consumers.
Description
Technical field
What the present invention relates to is a kind of preparation method of collagen-stabilized instant holothurian, and the preparation method that is specifically related to sea cucumber relates in particular to the selection of key parameter in manufacturing process.
Background technology
Sea cucumber ownership invertebrate, Echinodermata, Holothuroidea, the first order of shield.According to statistics, the whole world about You40Zhong， of edible sea cucumber China accounts for half, reaches 20 kinds.Trophic analysis shows, wall of sea cucumber Stichopus japonicus has better nutritivity and is worth, and wherein, collagen and degradation product thereof, fucosido chondroitin sulfate, fucosan and multiple saponin(e etc. are all important bioactivators.The traditional Chinese medical science proposes or prescription therapeutic tumour, alpastic anemia and diabetes etc. alone with sea cucumber and has all obtained good effect.The domestic and international also report of, blood coagulation resisting function antitumor relevant for sea cucumber, antithrombotic, antibacterial, antiviral, anti-oxidant, the anti-ageing aspect of waiting for a long time.In recent years, along with the day by day increasing of consumer to sea cucumber demand, the aquaculture of sea cucumber and processing industry are able to flourish.Yet, because sea cucumber is the very competent marine organisms of a kind of self-dissolving, in the last hundred years, the processing technology of sea cucumber is continued to use traditional dry process so that it circulates in distribution chain always, also there is in the last few years instant holothurian interview, but, the instant holothurian that major part floods market is to process with drying sea cucumber technique or after directly sending out with dried sea-cucumber water, because processing and water are sent out process and are made the loss of a large amount of nutrition and bioactive ingredients, and cause the change of sea cucumber local flavor, and the passive consumption status in " present good merchantable brand " always.And the sea cucumber of made of the present invention is except retaining its original nutrition and bioactive ingredients, also there is still good sea cucumber local flavor and institutional framework, be consumers in general, particularly person in middle and old age consumer praises highly.
Summary of the invention
Deficiency for prior art, the invention provides a kind of method of the instant holothurian of making delicious and nourishing and containing bioactivator by the technology of curing wall of sea cucumber Stichopus japonicus, the method is selected fresh and alive sea cucumbers, through pretreatment, solidify, the operation such as seasoning, packing, sterilizing makes.
Below introduce in detail the sea cucumber preparation method of a kind of nutrition and health care delicious food of the present invention,
A, pretreatment: by health, without the sea cucumber washing of self-dissolving, at anal side, therefrom suitably cut open the belly, thoroughly gill, wash and drain surface moisture, boiling 5-60 minute in 90-100 ℃, cooling rapidly.
B, solidify: by wall of sea cucumber Stichopus japonicus according to 1: the ratio of 1-10 joins in the neutral water soluble chitosan solution that contains 0.1-5mg/ml, vacuum impregnation 0.5-18 hour, vacuum in packaging bag is 60-200Pa, goes vacuum, and taking-up drains away the water, again according to 1: the ratio of 1-10 joins in transglutamin-ase 9 enzyme solutions floods 4-18 hour under 4-15 ℃ of condition, take out, clean and remove surface moisture, stand-by.
C, seasoning: by the wall of sea cucumber Stichopus japonicus being cured according to 1: the ratio of 0.1-10 is fully mixed with baste, dipping 5-30 minute, remove surperficial baste, stand-by.In baste, the content of each composition is salt 5-30%, sugared 5-30, and monosodium glutamate 2-10%, surplus is water.
D, nitrogen-filled packaging: for keeping quality and the form of sea cucumber, adopt vacuum gas displacement nitrogen-filled packaging.Vacuum in packaging bag is 1.0kPa, the nitrogen that substitution gas is 99.99%.Packing material is that composite plastic film is through heat seal or electromagnetic induction sealing.
E, sterilizing: adopt the sterilizing of high pressure steam sterilization tank, packaging bag free surface moisture is dried up after going out tank to warehouse-in.Under 0-4 ℃ of condition, can guarantee the quality 12 months, normal temperature 3 months.
Optimize, described washing water should meet national drinking water sanitary standard, and the shitosan using can derive from microorganism, animal and plant etc. for neutral soluble chitosan, TGase.
Advantage of the present invention:
1, adopt sea cucumber prepared by this curing technology to retain the original nutritional labeling of sea cucumber, bioactive ingredients, texture is soft, bullet, cunning, refreshing, tasty, has the distinctive local flavor of sea cucumber.
2, adopt sea cucumber prepared by this curing can within storage period, keep the distinctive quality of sea cucumber and local flavor, easy to carry, long shelf-life can enter distribution chain and consumption chain as other meat instant food.Therefore, be a kind of intensive processing product with high-tech content of sea cucumber.
the specific embodiment
Below in conjunction with embodiment, the present invention is described in further details.
Embodiment:
Get fresh and alive culturing sea cucumbers 10Kg, 1 kilogram of average 7-8 stature, cleans up, and with scissors, along anus direction from mid-sectional, thoroughly gills, and washes and drain surface moisture, and in boiling water, boiling is 15 minutes, cooling rapidly, removes surface water and gets wall of sea cucumber Stichopus japonicus.Ratio by wall of sea cucumber Stichopus japonicus according to 1: 10 joins in the neutral water soluble chitosan solution that contains 1mg/ml, vacuum impregnation 5 hours, vacuum is 100Pa, goes vacuum, and taking-up drains away the water, according to the ratio of 1: 10, join in transglutamin-ase 9 enzyme solutions and flood and spend the night under 4-15 ℃ of condition again, take out, clean and remove surface moisture, according to the ratio of 1: 10, fully mix with baste, dipping 5-30 minute, removes baste.For keeping quality and the form of sea cucumber, adopt vacuum gas displacement nitrogen-filled packaging.Vacuum in packaging bag is 1.0KPa, the nitrogen that substitution gas is 99.99%.Packing material is that composite plastic film is through heat seal or electromagnetic induction sealing.Adopt the sterilizing of high pressure steam sterilization tank, packaging bag free surface moisture is dried up after going out tank to warehouse-in.Under 0-4 ℃ of condition, can guarantee the quality 12 months.
Washing water described in this example should meet national drinking water sanitary standard, and the shitosan using can derive from microorganism, animal and plant etc. for neutral soluble chitosan, TGase.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to aforesaid operations, and all variations of making in essential scope of the present invention, remodeling, interpolation or replacement, all should belong to protection scope of the present invention.
Claims (1)
1. a preparation method for collagen-stabilized instant holothurian, is characterized in that:
A, pretreatment: by health, without the sea cucumber washing of self-dissolving, at anal side, therefrom suitably cut open the belly, thoroughly gill, wash and drain surface moisture, boiling 5-60 minute in 90-100 ℃, cooling rapidly;
B, curing: wall of sea cucumber Stichopus japonicus is joined in the neutral water soluble chitosan solution that contains 0.1-5mg/ml according to the ratio of 1:1-10, vacuum impregnation 0.5-18 hour, vacuum is 60-200Pa, go vacuum, taking-up drains away the water, then joins in transglutamin-ase 9 enzyme solutions and flood 4-18 hour under 4-15 ℃ of condition according to the ratio of 1:1-10, takes out, clean and remove surface moisture, stand-by;
C, seasoning: the wall of sea cucumber Stichopus japonicus being cured is fully mixed with baste according to the ratio of 1:0.1-10, and dipping 5-30 minute, removes surperficial baste, stand-by; In baste, the content of each composition is salt 5-30%, sugared 5-30%, and monosodium glutamate 2-15%, surplus is water;
D, nitrogen-filled packaging: for keeping quality and the form of sea cucumber, adopt vacuum gas displacement nitrogen-filled packaging; Vacuum in packaging bag is 1.0kPa, the nitrogen that substitution gas is 99.99%; Packing material is that composite plastic film is through heat seal or electromagnetic induction sealing;
E, sterilizing: adopt the sterilizing of high pressure steam sterilization tank, packaging bag free surface moisture is dried up after going out tank to warehouse-in.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910016953.6A CN101773260B (en) | 2009-06-30 | 2009-06-30 | Method for producing collagen-stabilized instant sea cucumbers |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910016953.6A CN101773260B (en) | 2009-06-30 | 2009-06-30 | Method for producing collagen-stabilized instant sea cucumbers |
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| Publication Number | Publication Date |
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| CN101773260A CN101773260A (en) | 2010-07-14 |
| CN101773260B true CN101773260B (en) | 2014-03-12 |
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| CN200910016953.6A Active CN101773260B (en) | 2009-06-30 | 2009-06-30 | Method for producing collagen-stabilized instant sea cucumbers |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101891963B (en) * | 2010-07-16 | 2012-03-28 | 中国海洋大学 | Modified fish skin collagen and preparation method |
| CN101972008B (en) * | 2010-09-30 | 2013-02-27 | 大连工业大学 | Method for making sea cucumber preserves |
| CN104013029B (en) * | 2014-06-20 | 2016-06-01 | 大连海晏堂生物有限公司 | A kind of instant fresh cucumber product and its preparation method |
| CN105265940B (en) * | 2015-03-11 | 2021-02-19 | 中国海洋大学 | High-temperature and high-pressure sea cucumber with stable storage and preparation method thereof |
| CN109007655A (en) * | 2018-08-06 | 2018-12-18 | 当代海洋生物科技（深圳）有限公司 | A kind of instant high-quality sea cucumber processing methods |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161790A (en) * | 1996-01-08 | 1997-10-15 | 味之素株式会社 | Edible micro-capsule and food containing same |
| CN101268840A (en) * | 2007-03-22 | 2008-09-24 | 丹东科健食品有限公司 | Method for preparing health care nutrition instant holothurian |
-
2009
- 2009-06-30 CN CN200910016953.6A patent/CN101773260B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161790A (en) * | 1996-01-08 | 1997-10-15 | 味之素株式会社 | Edible micro-capsule and food containing same |
| CN101268840A (en) * | 2007-03-22 | 2008-09-24 | 丹东科健食品有限公司 | Method for preparing health care nutrition instant holothurian |
Non-Patent Citations (4)
| Title |
|---|
| M.Carmen Gomez-Guillen.Effect of chitosan and microbial transglutaminase on the gel forming ability of horse mackerel (Trachurus spp.) muscle under high pressure.《Food Research International》.2005,第103-110页. * |
| Prospero Di Pierro.Transglutaminase-catalyzed preparation of chitosan–ovalbumin films.《Enzyme and Microbial Technology》.2007,第437-441页. * |
| Yongqin Zhang.Preparation of half-deacetylated chitosan by forced penetration and its properties.《Carbohydrate Polymers》.2006,第229-234页. * |
| 周小华等.壳聚糖-明胶共聚物的酶促合成及抑菌性质.《应用化学》.2008,第25卷(第3期),第334-339页. * |
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| CN101773260A (en) | 2010-07-14 |
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