CN101769894A - Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof - Google Patents
Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof Download PDFInfo
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- CN101769894A CN101769894A CN200810220630A CN200810220630A CN101769894A CN 101769894 A CN101769894 A CN 101769894A CN 200810220630 A CN200810220630 A CN 200810220630A CN 200810220630 A CN200810220630 A CN 200810220630A CN 101769894 A CN101769894 A CN 101769894A
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Abstract
The invention relates to an analyzing and detecting technology and a device thereof. The position of the tail end of a capillary separating channel or the position close to the same is taken as a detecting area; the detecting area is provided with a coaxial magnetic induction coil; and a magnetic circuit of the magnetic induction coil is coaxial to the capillary separating channel. The induction coil is connected with an alternating-current signal generator so as to measure obtained signals of the induction coil, and is connected with a recording instrument after signal amplification. The invention has the advantages of better universality, uneasy pollution, simpler and more convenient application and the like.
Description
Technical field
The present invention relates to a kind of analysis and detection technology and relevant apparatus.Be specifically related to a kind of detection method and corresponding device thereof thereof of Capillary Electrophoresis.
Background technology
Capillary Electrophoresis is the electrophoresis that carries out in the very high kapillary of radiating efficiency, can utilize high voltage, has improved separating effect significantly, has efficient, low consumption, fast and characteristics such as be widely used.
Detection technique is one of core technology in capillary electrophoretic analysis method, directly influences detected object, detection sensitivity, the range of linearity and instrument cost.Present detection technique mainly contains ultraviolet absorption method, laser inductive fluorescence method, conductance method, ampere method, mass spectroscopy etc.Wherein, it is more general that uv absorption detection and electricity are led detection, but sensitivity is lower; Laser-Induced Fluorescence Detection and Amperometric Detection Coupled sensitivity are higher, but determination object is few; And mass spectroscopy relates to expensive instrument, and cost is very high.
Yet there are no the report of relevant Capillary Electrophoresis magnetic permeance detecting method and magnetic conductance detecting device.
Summary of the invention
The purpose of this invention is to provide that a kind of technology is simple, easy to use, the capillary electrophoresis coaxial type magnetic conductance detection method and the corresponding device thereof thereof of better performances.
Ultimate principle of the present invention is: material all has certain magnetic property.Select suitable separating medium, the magnetic conduction ability of tested component and separating medium be there are differences, at the terminal of capillary separation channels or near measuring this regional magnetic induction in the end.When the tested component stream after separating was regional after measured, magnetic induction changed.The magnetic induction size variation of record compartment analysis process can obtain separating spectrum.
Basic technical scheme of the present invention is: with the terminal of capillary separation channels or near the end is surveyed area, establishes a coaxial-type magnetic induction coil at surveyed area, and the magnetic circuit of magnetic induction coil is coaxial with capillary separation channels.Inductive coil is connected with AC signal generator, measures the signal by the gained of inductive coil, amplifies the back through signal and links to each other with registering instrument.
Below the present invention is explained in detail.
Magnetic conductance pick-up unit of the present invention comprises kapillary, magnet ring, inductive coil, AC signal generator and signal measurement apparatus.Inductive coil is wound in capillary separation channels proximal end place by enameled wire and makes, and width is less than or equal to 2mm, and at its peripheral tight cover one magnet ring, forms magnetic inductor jointly.Two end interfaces of coil respectively with AC signal generator and signal measurement apparatus.Tested component stream after separating is when the magnetic induction zone, and magnetic induction changes, and the signal by inductive coil is access to registering instrument through the output terminal of signal amplifier.
Inductive coil among the present invention and intercapillary structural relation have two kinds, and the one, directly be wound on the kapillary with enameled wire, form integrated with kapillary; The 2nd, make inductive coil and kapillary independent, earlier enameled wire is wrapped in a diameter and kapillary is close and can tightly be enclosed within on the pipe box on the kapillary and make coil, be set on the kapillary conveniently replaced coil or kapillary again.
Advantage of the present invention and good effect are to have good versatility.Because all materials all have certain magnetic property, as long as select suitable separating medium, the magnetic conduction ability of each component and separating medium be there are differences, just can response all be arranged to various components.
In addition, the present invention does not cause electrode fouling owing to electrode contacts with solution like that as Electrochemical Detection; Do not resemble yet and to scrape off the outer protective seam of capillary separation channels the optical detection and make it easily to fracture.The present invention is easy to assembling, cost is low, easy to use.
Description of drawings
Accompanying drawing is the structural representation of capillary electrophoresis coaxial type magnetic conductance pick-up unit of the present invention.
Among the figure, 1 is kapillary, and 2 is magnet ring, and 3 is coil, and 4 is AC signal generator, and 5 is resistance, and 6 is voltage measuring apparatus, and 7 is signal amplifier, and 8 is registering instrument.
Embodiment
Enumerate part embodiment below.
Fig. 1 is the device that the magnetic conductance of the coaxial type of Capillary Electrophoresis detects.Kapillary (1) internal diameter 0.1cm, external diameter 0.36cm, long 70cm is as split tunnel.Enameled wire with Φ 0.17 is made coil (3), coil width 0.1～0.2cm at the zone of kapillary proximal end 8.0～8.2cm winding 300～1200 circles.The magnet ring (2) of internal diameter 1.2～2.0cm is placed in outside the coil, forms magnetic inductor, the magnetic circuit direction is coaxial with capillary separation channels.In order to measure signal magnitude by inductive coil, coil (3) be access to AC signal generator (4) after the resistance (5) of a 1～10k is connected.Resistance (5) two termination voltage measuring apparatus (6), the measured signal of voltage measuring apparatus (6) is used registering instrument (8) record after amplifier (7) amplifies.
The frequency of utilization of AC signal generator (4) is 200k～15MHz, and voltage measuring apparatus (6) is used the high-frequency electronic milivoltmeter.
According to the number of turn of coil (3) and the frequency of utilization size of AC signal generator (4), suitably adjust resistance (5) size, make it impedance matching, to obtain signal to noise ratio (S/N ratio) preferably.
Coil (3) can directly be wrapped on the kapillary (1), also can independent winding pipe puts to make coil, is set to kapillary again on kapillary (1).
The outer magnet ring (2) that is with of coil (3).
The invention is not restricted to the foregoing description.Measurement is by the signal magnitude of inductive coil, also can be with other mode; Component parameter also can be done suitable adjustment.
Claims (4)
1. capillary electrophoresis coaxial type magnetic permeance detecting method, it is characterized in that, establish a coaxial-type magnetic induction coil at the surveyed area at kapillary proximal end place, the magnetic circuit of magnetic induction coil is coaxial with capillary separation channels, inductive coil is connected with AC signal generator, measures the signal by the gained of inductive coil, and signal amplifies the back and links to each other with registering instrument, the magnetic induction size variation of record compartment analysis process obtains separating spectrum.
2. capillary electrophoresis coaxial type magnetic conductance pick-up unit, comprise that kapillary (1), coil (3), AC signal generator (4), resistance (5), voltage measuring apparatus (6) and signal are through amplifier (7), it is characterized in that, coil (3) is with the surveyed area that is located in kapillary (1), coil (3) be access to AC signal generator (4) after resistance (5) is connected, resistance (5) two termination voltage measuring apparatus (6), the output of voltage measuring apparatus (6) connects signal through amplifier (7), and signal is through the output record instrument (8) of amplifier (7).
3. capillary electrophoresis coaxial type magnetic conductance pick-up unit according to claim 2 is characterized in that, coil (3) width is 0.1～0.2cm.
4. capillary electrophoresis coaxial type magnetic conductance pick-up unit according to claim 2 is characterized in that, the outer magnet ring (2) that is with of exterior loop (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810220630A CN101769894A (en) | 2008-12-31 | 2008-12-31 | Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810220630A CN101769894A (en) | 2008-12-31 | 2008-12-31 | Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof |
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| Publication Number | Publication Date |
|---|---|
| CN101769894A true CN101769894A (en) | 2010-07-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200810220630A Pending CN101769894A (en) | 2008-12-31 | 2008-12-31 | Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108362756A (en) * | 2018-01-05 | 2018-08-03 | 复旦大学 | Self-positioning capillary electrophoretic ampere detecting tank of a kind of magnetic force assembling and preparation method thereof |
| CN110187001A (en) * | 2019-05-22 | 2019-08-30 | 西红柿科技（武汉）有限公司 | A kind of defect detecting device and detection method using surface magnetic conductance technology |
-
2008
- 2008-12-31 CN CN200810220630A patent/CN101769894A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108362756A (en) * | 2018-01-05 | 2018-08-03 | 复旦大学 | Self-positioning capillary electrophoretic ampere detecting tank of a kind of magnetic force assembling and preparation method thereof |
| CN108362756B (en) * | 2018-01-05 | 2020-07-28 | 复旦大学 | Magnetic assembly self-positioning capillary electrophoresis ampere detection tank and preparation method thereof |
| CN110187001A (en) * | 2019-05-22 | 2019-08-30 | 西红柿科技（武汉）有限公司 | A kind of defect detecting device and detection method using surface magnetic conductance technology |
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Application publication date: 20100707 |