CN101766654A - Method for identifying Chinese herbal medicine by using electrochemistry - Google Patents
Method for identifying Chinese herbal medicine by using electrochemistry Download PDFInfo
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- CN101766654A CN101766654A CN200910254498A CN200910254498A CN101766654A CN 101766654 A CN101766654 A CN 101766654A CN 200910254498 A CN200910254498 A CN 200910254498A CN 200910254498 A CN200910254498 A CN 200910254498A CN 101766654 A CN101766654 A CN 101766654A
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Abstract
The invention relates to a method for identifying Chinese herbal medicine by using electrochemistry. The method comprises the following steps: pulverizing Chinese herbal medicine into powder, adding to a mixture (a finite concentration) of sulfuric acid, manganese sulfate, acetone and potassium bromate, and completely stirring to carry out a chemical oscillating reaction, thereby obtaining a mixed solution; transferring the mixed solution to an electrochemical working instrument with an oxidation-reduction electrode, starting to measure and record, and drawing a reaction map; converting the reaction map into data by the electrochemical working instrument, recording and storing the data, thereby obtaining the map data of the Chinese herbal medicine; and changing the amount of the Chinese herbal medicine with the state of the chemical oscillator unchanged to obtain a series of data, drawing an amplitude-concentration curve chart, and qualitatively and quantitatively identifying the Chinese herbal medicine based on the chart which directly reflects the relationship between the amplitude and the concentration. The novel method for identifying Chinese herbal medicine is scientific and effective, has the advantages of simple operation and convenient mastery, and is beneficial to accelerating the modernization of the Chinese herbal medicine.
Description
Technical field
The present invention relates to a kind of method of utilizing electrochemistry to differentiate Chinese herbal medicine.
Background technology
As everyone knows, Chinese herbal medicine is of a great variety, many medium-height grass prescriptions carry out comparatively difficulty of identification from aspects such as phytomorph, medical material shape, nature and flavor, color and lusters to it, people have probed into many methods, but because the complex chemical composition of Chinese herbal medicine, existing several chemicals detection methods can't be satisfactory for Chinese herbal medicine at present.The Chinese herbal medicine chemical information is that the composite factor by the number of chemical composition constitutes.With Chinese crude drug, fitochemical studies and pharmacological effect research through century more than one, people more and more recognize, the drug effect of Chinese herbal medicine is not from any one single active component, and be the various active composition basically, or even the synthesis result that produces with " synergism " of non-active ingredient or " giving birth to the gram effect ", monarch etc.Therefore the chemical information in the Chinese herbal medicine has certain ambiguity, has only part chemical information (comprise known active component, known non-active ingredient, a part is principal component not) to characterize in finger printing.What research was more at present is to utilize high performance liquid chromatography (HPLC) to set up the finger printing of Chinese herbal medicine.The employed instrument costliness of HPLC method, post pre-treatment process complexity during analytic sample, experimental result and repeatability are had relatively high expectations to the experimenter.Data shows, this finger printing is difficult to reflect the information of Chinese herbal medicine or medicament comprehensively, even can not indicate the source of this composition, all contain little bavin alkali as Rhizoma Coptidis mutually with yellow, if only just be difficult to they differentiations with the index of little bavin alkali as discriminating Rhizoma Coptidis and Cortex Phellodendri.When detecting the Chinese patent medicine ZHIBAI DIHUANG WAN for another example, all can detect little bavin alkali owing to adopt Radix Scutellariae or directly adopt the little bavin alkali that extracts to feed intake, but the Chinese patent medicine that this method makes not a ZHIBAI DIHUANG WAN.This just requires us must obtain the finger printing of Chinese medicines such as Rhizoma Coptidis, yellow phase, Huang Fen, is contrasted then to determine little bavin alkali source, and obviously, this method has its helpless and defective.
Summary of the invention
The technical problem that the present invention solves: a kind of method of utilizing electrochemistry to differentiate Chinese herbal medicine is provided, utilize the chemical oscillation technology sensitive, that selectivity is strong to obtain Chinese herbal medicine oscillation characteristics collection of illustrative plates, take all factors into consideration that mixed system is multi-component to influence each other, make the challenge simplification that becomes, experience problem is scientific.Overcome the drawback that the resulting finger printing of present other method can not show the medicinal herb components source simultaneously, Chinese herbal medicine characteristic and synergism really are embodied among the oscillation characteristics collection of illustrative plates, can differentiate various medical herbs simply and effectively, and simple to operate, be convenient to grasp.
The technical solution used in the present invention:
1, utilize electrochemical method determining to write down the spectrum data of various medical herbs.
(1) gets first kind of Chinese herbal medicine, and it is ground into 〉=100 purpose powder;
(2) take by weighing a certain amount of said herbal medicine powder and join in certain density sulphuric acid, manganese sulfate, acetone and the Potassium bromate. mixed liquor, abundant stirring is carried out chemical oscillating reaction and is obtained mixed solution;
(3) mixed solution is changed in the electrochemistry working instrument that has oxidation-reduction electrode and begin to measure record, and draw the reaction collection of illustrative plates;
(4) by in the electrochemistry working instrument collection of illustrative plates being converted to data, records store data just can obtain the spectrum data of first kind of Chinese herbal medicine;
(5) get second kind of single Chinese herbal medicine, keep chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain the spectrum data of second kind of Chinese herbal medicine; Circulation just can obtain differentiating the spectrum data of various medical herbs according to this.
Described step (2) is: at first a certain amount of herbal powder adding is had in the jacketed reactor of agitating device, then certain density sulphuric acid, manganese sulfate, acetone and Potassium bromate. are added in the jacketed reactor successively, under 25 ℃ ± 0.5 ℃ temperature, fully stir and carry out chemical oscillating reaction and obtain mixed solution;
Described sulfuric acid concentration is 1.104mol/L, and manganese sulfate concentration is 0.016mol/L, and the concentration of acetone is 0.108mol/L, and the concentration of Potassium bromate. is 0.04mol/L.
2, draw amplitude-concentration curve.
Keeping chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, change the addition of herbal powder, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain a series of data, draw Chinese herbal medicine amplitude-concentration curve, can intuitively reflect the relation of amplitude and concentration, carry out qualitative and quantitatively evaluation Chinese herbal medicine by figure.
Advantage that the present invention compared with prior art has and effect:
1, the invention provides a kind of new, scientific and effective Chinese herbal medicine discrimination method, can differentiate various medical herbs simply and effectively, and simple to operate, be convenient to grasp.Make the discriminating of Chinese herbal medicine scientific, oversimplify.
2, the present invention brings new research method and research thinking will for the synthetic or the like of Study on mechanism, the determination of active ingredients of Chinese herbal medicine, medicinal coordination compound, accelerates the Chinese herbal medicine modernization process.
Description of drawings
Fig. 1 is the chemical oscillation finger printing of Rhizoma Chuanxiong,
Fig. 2 is the amplitude-concentration curve of Rhizoma Chuanxiong.
The specific embodiment
1,2 specific embodiments of the invention are described in conjunction with the accompanying drawings.
1, utilize electrochemical method determining to write down the spectrum data of various medical herbs.
(1) gets first kind of Chinese herbal medicine, and it is ground into 〉=100 purpose powder;
(2) taking by weighing a certain amount of said herbal medicine powder adding has in the jacketed reactor of agitating device, then certain density sulphuric acid (1.104mol/L), manganese sulfate (0.016mol/L), acetone (0.108mol/L) and Potassium bromate. (0.04mol/L) are added in the jacketed reactor successively, under 25 ℃ ± 0.5 ℃ temperature, fully stir and carry out chemical oscillating reaction and obtain mixed solution;
(3) the interior bed device of unlatching jacketed reactor changes mixed solution in the electrochemistry working instrument that has oxidation-reduction electrode over to and begins to measure record, and draws the reaction collection of illustrative plates;
(4) by the electrochemistry working instrument collection of illustrative plates is converted to data, records store data just can obtain the spectrum data of first kind of Chinese herbal medicine;
(5) get second kind of single Chinese herbal medicine, keep chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain the spectrum data of second kind of Chinese herbal medicine; Circulation just can obtain differentiating the spectrum data of various medical herbs according to this.
The spectrum data that adopts said method to measure 10 kinds of Chinese herbal medicine of record sees Table 1.For example shown that the addition of Pinellia Tuber is 0.4g in the table, the addition of sulphuric acid (1.104mol/L), manganese sulfate (0.016mol/L), acetone (0.108mol/L) and Potassium bromate. (0.04mol/L) is 50ml.The spectrum data of measuring record is max0.8954, min0.1915.
The spectrum data of table 1:10 kind Chinese herbal medicine
| Title | Concentration (g/50ml) | ??max | ??min |
| The Rhizoma Pinelliae | ??0.4 | ??0.8954 | ??0.1915 |
| Radix Stephaniae Tetrandrae | ??0.4 | ??0.535 | ??0.32 |
| Radix Ophiopogonis | ??0.3 | ??0.4821 | ??0.2991 |
| Pericarpium Citri Reticulatae Viride | ??0.3 | ??0.6636 | ??0.2182 |
| Ramulus Mori | ??0.5 | ??0.5696 | ??0.2768 |
| Caulis Sargentodoxae | ??0.6 | ??0.5192 | ??0.104 |
| Rhizoma Paridis | ??0.4 | ??0.6067 | ??0.3237 |
| Polyporus | ??0.2 | ??0.9877 | ??0.114 |
| Radix Paeoniae Rubra | ??0.3 | ??0.7477 | ??0.1817 |
| Rhizoma Chuanxiong | ??0.4 | ??0.4711 | ??0.2918 |
2, draw Chinese herbal medicine amplitude-concentration curve.
Keeping chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, change the addition of herbal powder, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain a series of data, draw Chinese herbal medicine amplitude-concentration curve, can intuitively reflect the relation of amplitude and concentration, carry out qualitative and quantitatively evaluation Chinese herbal medicine by figure.For example, Fig. 2 is for measuring the Rhizoma Chuanxiong amplitude-concentration curve of the spectrum data drafting of writing down Rhizoma Chuanxiong by this method.
3, discrimination method:
(1) qualitative identification: aspects such as first phytomorph according to Chinese herbal medicine, medical material shape, nature and flavor, color and luster are tentatively distinguished, find out corresponding Chinese herbal medicine addition according to the title synopsis of tentatively distinguishing 1, utilize electrochemical method determining to write down the spectrum data of this Chinese herbal medicine then, if the data of measuring are identical with the data in this Chinese herbal medicine table 1, the title of so tentatively distinguishing is exactly the accurate title of this Chinese herbal medicine.If not, synopsis 1 is found out Chinese herbal medicine proximate with it and is determined addition again, continues to measure, and until finding the Chinese herbal medicine identical with data in the table 1, finishes the qualitative identification of Chinese herbal medicine.
(2) quantitative identification: get Rhizoma Chuanxiong and utilize electrochemical method determining to write down the spectrum data of this Chinese herbal medicine, calculate the average data at first three peak of collection of illustrative plates, and find out the Chinese herbal medicine reaction density corresponding with these data by the amplitude-concentration curve of Rhizoma Chuanxiong, finish the quantitative identification of Chinese herbal medicine.This method also can be used for the discriminating of Chinese herbal medicine Maturity.
Claims (4)
1. method of utilizing electrochemistry to differentiate Chinese herbal medicine is characterized in that:
(1) gets first kind of Chinese herbal medicine, and it is ground into 〉=100 purpose powder;
(2) take by weighing a certain amount of said herbal medicine powder and join in certain density sulphuric acid, manganese sulfate, acetone and the Potassium bromate. mixed liquor, abundant stirring is carried out chemical oscillating reaction and is obtained mixed solution;
(3) mixed solution is changed in the electrochemistry working instrument that has oxidation-reduction electrode and begin to measure record, and draw the reaction collection of illustrative plates;
(4) by in the electrochemistry working instrument collection of illustrative plates being converted to data, records store data just can obtain the spectrum data of first kind of Chinese herbal medicine;
(5) get second kind of single Chinese herbal medicine, keep chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain the spectrum data of second kind of Chinese herbal medicine; Circulation just can obtain differentiating the spectrum data of various medical herbs according to this.
2. a kind of method of utilizing electrochemistry to differentiate Chinese herbal medicine according to claim 1, it is characterized in that described step (2) is: at first a certain amount of herbal powder adding is had in the jacketed reactor of agitating device, then certain density sulphuric acid, manganese sulfate, acetone and Potassium bromate. are added in the jacketed reactor successively, under 25 ℃ ± 0.5 ℃ temperature, fully stir and carry out chemical oscillating reaction and obtain mixed solution.
3. a kind of method of utilizing electrochemistry to differentiate Chinese herbal medicine according to claim 1 and 2, it is characterized in that: described sulfuric acid concentration is 1.104mol/L, manganese sulfate concentration is 0.016mol/L, and the concentration of acetone is 0.108mol/L, and the concentration of Potassium bromate. is 0.04mol/L.
4. a kind of method of utilizing electrochemistry to differentiate Chinese herbal medicine according to claim 1 and 2, it is characterized in that: keeping chemical oscillator sulphuric acid, manganese sulfate, acetone and Potassium bromate. concentration unanimity, the molten sample time is identical, reaction temperature is constant, under the constant condition of response time, change the addition of herbal powder, adopt same electrochemistry working instrument, repeating step (1) (2) (3) (4) just can obtain a series of data, draw amplitude-concentration curve, can intuitively reflect the relation of amplitude and concentration by figure, Chinese herbal medicine is carried out qualitative and quantitatively identifies.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323306A (en) * | 2011-05-18 | 2012-01-18 | 北京兴有丰科科技发展有限公司 | With the direct qualitative and quantitative analysis of chemical oscillation to L-tryptophane in the compound amino acid |
| CN102565169A (en) * | 2012-01-05 | 2012-07-11 | 查孝柱 | Electrochemical fingerprint chromatogram identification method for edible oil |
| CN102818827A (en) * | 2012-08-24 | 2012-12-12 | 安徽大学 | Natural antioxidant detecting method |
| CN108333232A (en) * | 2018-01-16 | 2018-07-27 | 吉林省吴太感康药业有限公司 | A kind of detection method of the electrochemistry finger-print of calculus bovis factitius |
| CN108680621A (en) * | 2018-05-21 | 2018-10-19 | 江苏省中国科学院植物研究所 | A kind of floristic discrimination method |
| CN109187673A (en) * | 2018-10-24 | 2019-01-11 | 杭州电子科技大学 | A kind of plant Relationship iden- tification method based on pollen |
| CN109374698A (en) * | 2018-11-06 | 2019-02-22 | 杭州电子科技大学 | A method of based on the 2.5D electrochemistry fingerprint identification fleece-flower root or RADIX POLYGONI MULTIFLORI PREPARATA |
| CN111579621A (en) * | 2020-05-27 | 2020-08-25 | 四川国康药业有限公司 | Method for detecting astragalus membranaceus tablets by chemical oscillation fingerprint spectrum |
| CN112903791A (en) * | 2021-01-19 | 2021-06-04 | 天津中医药大学 | Method for identifying gum medicinal material based on chemical oscillation fingerprint spectrum technology |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102323306A (en) * | 2011-05-18 | 2012-01-18 | 北京兴有丰科科技发展有限公司 | With the direct qualitative and quantitative analysis of chemical oscillation to L-tryptophane in the compound amino acid |
| CN102565169A (en) * | 2012-01-05 | 2012-07-11 | 查孝柱 | Electrochemical fingerprint chromatogram identification method for edible oil |
| CN102565169B (en) * | 2012-01-05 | 2014-06-25 | 岳西县光圣茶油有限公司 | Electrochemical fingerprint chromatogram identification method for edible oil |
| CN102818827A (en) * | 2012-08-24 | 2012-12-12 | 安徽大学 | Natural antioxidant detecting method |
| CN108333232A (en) * | 2018-01-16 | 2018-07-27 | 吉林省吴太感康药业有限公司 | A kind of detection method of the electrochemistry finger-print of calculus bovis factitius |
| CN108680621A (en) * | 2018-05-21 | 2018-10-19 | 江苏省中国科学院植物研究所 | A kind of floristic discrimination method |
| CN109187673A (en) * | 2018-10-24 | 2019-01-11 | 杭州电子科技大学 | A kind of plant Relationship iden- tification method based on pollen |
| CN109374698A (en) * | 2018-11-06 | 2019-02-22 | 杭州电子科技大学 | A method of based on the 2.5D electrochemistry fingerprint identification fleece-flower root or RADIX POLYGONI MULTIFLORI PREPARATA |
| CN111579621A (en) * | 2020-05-27 | 2020-08-25 | 四川国康药业有限公司 | Method for detecting astragalus membranaceus tablets by chemical oscillation fingerprint spectrum |
| CN111579621B (en) * | 2020-05-27 | 2023-07-14 | 四川国康药业有限公司 | Method for detecting astragalus membranaceus slices by chemical oscillation fingerprint |
| CN112903791A (en) * | 2021-01-19 | 2021-06-04 | 天津中医药大学 | Method for identifying gum medicinal material based on chemical oscillation fingerprint spectrum technology |
| CN112903791B (en) * | 2021-01-19 | 2023-02-24 | 天津中医药大学 | Method for identifying gum medicinal material based on chemical oscillation fingerprint spectrum technology |
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Application publication date: 20100707 |