CN101084974A - Quality control method for codonopsis pilosula - Google Patents
Quality control method for codonopsis pilosula Download PDFInfo
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- CN101084974A CN101084974A CN 200610014203 CN200610014203A CN101084974A CN 101084974 A CN101084974 A CN 101084974A CN 200610014203 CN200610014203 CN 200610014203 CN 200610014203 A CN200610014203 A CN 200610014203A CN 101084974 A CN101084974 A CN 101084974A
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Abstract
The invention relates to a quality control method of traditional Chinese medicinal materials especially to a quality control method of radix codonopsitis. The method comprises the following steps (a) establishment of contrast finger print of radix codonopsitis: first of all preparing control sample solution of radix codonopsitis, then detecting contrast finger print of radix codonopsitis; (b) detection of finger print of radix codonopsitis for assay: detecting the finger print of radix codonopsitis according to the steps and conditions in step a; (c) comparing the finger prints, recognizing the quantity of their common absorption peak to determine if the product quality is qualified.
Description
Technical field
The present invention relates to a kind of method of quality control of Chinese crude drug, particularly relate to the method for quality control of codonopsis pilosula.
Background technology
Radix Codonopsis (Codonopsis pilosula) belongs to campanulaceae (Platycodon) plant, ancient times with originate in prefecture, Chinese Shangdang for the famousst and precious, so the title Radix Codonopsis.In decades, on original research basis, both at home and abroad the Radix Codonopsis chemical constituent is furtherd investigate, from Radix Codonopsis isolation identification 10 kinds of sterols, 21 kinds of glucosides classes, 5 kinds of alkaloidss and nitrogen containing component, 34 kinds of volatile oil components, 13 kinds of triterpeness and other constituents also have the inorganic elements and the aminoacid of multiple needed by human.
Chinese medicine fingerprint is meant chromatograph or spectrographic collection of illustrative plates common, that have distinctive certain class or number constituents in certain Chinese crude drug or the Chinese patent medicine.Do not have under the clear and definite situation in the present stage Effective Components of Chinese Herb overwhelming majority, Chinese medicine fingerprint has great importance for the quality of effective control Chinese crude drug or Chinese patent medicine.The Japan main manufacturing enterprise of Chinese prescription medicine just adopts the high-efficiency liquid-phase fingerprint control of quality in enterprises in the eighties in 20th century.Germany, France find that the medical function of Folium Ginkgo extract is extract gained material group's mass action result in the process that Folium Ginkgo extract is developed jointly, and to the quality control of such integral body, also adopt the high-efficiency liquid-phase fingerprint method.In the plant medical herbs guide of formulating U.S. FDA recent years clearly the method for quality control (FDA.Guidance of Industry:Botanical Drug (Draft) .2000August) of finger printing as the compounding substances group.Along with going deep into of research, it is found that, as the product of putting into practice of theory of Chinese medical science, Chinese medicine, especially herbal mixture, wherein contained arbitrary composition all can not be represented its whole curative effect.People recognize that gradually the existing quality standard with reference to Western medicine (synthetic drug) quality control pattern can not reflect the intrinsic quality of Chinese medicine rightly.From development trend, from existing quality control pattern to a kind of comprehensive, macroscopic, quantifiable discriminating combines with main active constituent content measuring is the trend that develops.
The working standard of medicine quality evaluated is to utilize spectrum or the discriminating of chromatograph means and measure a certain or several effective ingredient, active component or index components, and the routine examination project of pharmacopeia regulation.Record 602 kinds of medical materials and patent medicine kind altogether as Chinese Pharmacopoeia 2000 version [an one].Wherein have 992 thin layer chromatographys to differentiate that 308 kinds have assay (volumetric method, spectrographic method, liquid chromatography, gas chromatography and TLC scanning method), most of kinds have general inspection item.Obviously. the setting of these quality standards is the patterns of having imitated chemical drugs.The German medical herbs monograph that other country edits as the HANYAO in Britain, India, U.S.'s medical herbs allusion quotation, the Pharmacopeia of Japan and German CommissionE etc. has also adopted essentially identical content.For chemical drugs, its active ingredient is the unification compound of clear in structure, and structure activity relationship is clear and definite, and its content and purity are directly expressed it and effectively reached safety.Yet the characteristics of middle medical drugs are compound compatibilities, and any single content height effective or active component all can not be expressed its whole curative effect.For example, the contained astragaloside (aastraga losideIV) of the Radix Astragali is the current discriminating of quality standard and the most common target of assay of being selected as, but not according to clearly getting in touch that the function that proves the astragaloside and the Radix Astragali cures mainly.Equally, Rhizoma Coptidis, Cortex Phellodendri, Radix Berberidis all contain girder alkali, and be general all with its target as detection, completely different but three's function cures mainly.The situation of compound preparation is just complicated more.The traditional Chinese medical science is this not to be that man-to-man nonlinear theory and practice explanation Chinese medicine quality should adopt certain macroscopic comprehensive quality evaluation means.
Summary of the invention
The object of the present invention is to provide a kind of method of quality control that can the overall merit codonopsis pilosula.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of quality control of codonopsis pilosula comprises the steps:
(a). the foundation of Radix Codonopsis reference fingerprint
The preparation of codonopsis pilosula control sample solution:
Take by weighing the medical material fine powder, add methanol solution, supersound process is put coldly, shakes up, and 0.45 μ m microporous filter membrane filters;
The mensuration of codonopsis pilosula reference fingerprint:
Draw above-mentioned control sample solution and inject chromatograph of liquid, use high performance liquid chromatography to measure, obtain the codonopsis pilosula reference fingerprint, chromatographic condition: chromatographic column is filler with the octadecylsilane chemically bonded silica; Adopt gradient elution, mobile phase is the acetonitrile phosphate aqueous solution; Detect wavelength 200～210nm;
(b). the mensuration of codonopsis pilosula product fingerprint collection of illustrative plates to be measured:
Get codonopsis pilosula product to be measured, measure the finger printing of this product to be measured according to Step By Condition described in above-mentioned (a);
(c). described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are compared, discern the quantity of the two common absworption peak that has, to determine whether product quality is qualified.
Preferred chromatographic condition is in described (a) step: the flow velocity 0.500～1.500ml/min of mobile phase, detect wavelength 200～205nm, 20～40 ℃ of column temperatures.
Following method is adopted in the foundation of preferred codonopsis pilosula finger printing:
(a). the preparation method of codonopsis pilosula control sample solution: precision takes by weighing the medical material fine powder, put in the tool plug triangular flask, the accurate methanol solution that adds, the accurate title, decided supersound process 30 minutes, put cold, claim again to decide weight, add methanol and supply the weight that subtracts mistake, shake up, 0.45 μ m microporous filter membrane filters, and gets subsequent filtrate as need testing solution;
The mensuration of codonopsis pilosula reference fingerprint:
Chromatographic condition: mobile phase A is 0.02% phosphate aqueous solution; Mobile phase B is the phosphate aqueous solution of 80% acetonitrile 0.02%;
The gradient elution program is as shown in the table:
| Time (min) | Flow velocity (ml/min) | Acetonitrile (%) | 0.05% phosphoric acid (%) |
| 0 30 45 65 100 | 1.0 1.0 1.0 1.0 1.0 | 15 25 40 80 80 | 85 75 60 20 20 |
(b). the mensuration of codonopsis pilosula product fingerprint collection of illustrative plates to be measured:
Get codonopsis pilosula product to be measured, measure the finger printing of this product to be measured according to Step By Condition described in above-mentioned (a);
(c). described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are compared, discern the quantity of the two common absworption peak that has, to determine whether product quality is qualified.
In the foundation of reference fingerprint, in the reference fingerprint of the use codonopsis pilosula that high effective liquid chromatography for measuring obtained 11 absworption peaks are arranged, the absworption peak that wherein unimodal area surpasses total peak area 10% has 2, is respectively:
No. 10 peaks, average retention time RT is 84.452min, and RSD is 0%, and peak area is 1275.39, and RSD is 3%;
No. 11 peaks, average retention time RT is 94.316min, and RSD is 0%, and peak area is 3319.528, and RSD is 3.42%.
In the foundation of reference fingerprint, in the reference fingerprint of the use codonopsis pilosula that high effective liquid chromatography for measuring obtained 11 absworption peaks are arranged, the absworption peak that wherein unimodal area surpasses total peak area 5% has 4, is respectively:
No. 2 peaks, average retention time RT is 12.068min, and RSD is 0.53%, and peak area is 381.699, and RSD is 11.9%;
No. 3 peaks, average retention time RT is 12.711min, and RSD is 0.4%, and peak area is 634.231, and RSD is 14.32%;
No. 10 peaks, average retention time RT is 84.452min, and RSD is 0%, and peak area is 1275.39, and RSD is 3%;
No. 11 peaks, average retention time RT is 94.316min, and RSD is 0%, and peak area is 3319.528, and RSD is 3.42%.In the foundation of reference fingerprint, in the reference fingerprint of the use codonopsis pilosula that high effective liquid chromatography for measuring obtained 11 absworption peaks are arranged, the absworption peak that wherein unimodal area surpasses total peak area 2% has 11, is respectively:
No. 1 peak, average retention time RT is 7.308, and RSD is 0.34, and peak area is 289.896, and RSD is 8.94
No. 2 peaks, average retention time RT is 12.068, and RSD is 0.53, and peak area is 381.699, and RSD is 11.9
No. 3 peaks, average retention time RT is 12.711, and RSD is 0.4, and peak area is 634.231, and RSD is 14.32
No. 4 peaks, average retention time RT is 14.148, and RSD is 0.32, and peak area is 355.482, and RSD is 2.2
No. 5 peaks, average retention time RT is 21.677, and RSD is 0.35, and peak area is 270.857, and RSD is 35.46
No. 6 peaks, average retention time RT is 35.544, and RSD is 0.17, and peak area is 227.295, and RSD is 25.29
No. 7 peaks, average retention time RT is 36.097, and RSD is 0.21, and peak area is 293.056, and RSD is 10.03
No. 8 peaks, average retention time RT is 39.207, and RSD is 0.17, and peak area is 228.713, and RSD is 2.74
No. 9 peaks, average retention time RT is 45.348, and RSD is 0, and peak area is 282.564, and RSD is 4.46
No. 10 peaks, average retention time RT is 84.452, and RSD is 0, and peak area is 1275.39, and RSD is 3
No. 11 peaks, average retention time RT is 94.316, and RSD is 0, and peak area is 3391.528, and RSD is 3.42.
The present invention adopts the quality of following method control codonopsis pilosula, get and treat codonopsis pilosula, adopt with the identical method of preparation method, chromatographic condition, assay method of codonopsis pilosula control sample and measure, obtain finger printing, codonopsis pilosula product fingerprint collection of illustrative plates to be measured and codonopsis pilosula are compared according to finger printing, when the two finger printing has absworption peak identical more than 3 or 3, think that just codonopsis pilosula product to be measured is qualified products.
Preferably, codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint relatively when the two finger printing has absworption peak identical more than 5 or 5, are thought the up-to-standard of described codonopsis pilosula product to be measured.
Comparatively preferably, codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint relatively when the two finger printing has absworption peak identical more than 7 or 7, are thought the up-to-standard of described codonopsis pilosula product to be measured.
The most preferably, tested codonopsis pilosula product fingerprint collection of illustrative plates and contrast codonopsis pilosula finger printing relatively when the two finger printing has 8 identical absworption peaks, are thought the up-to-standard of described codonopsis pilosula product to be measured.
The present invention does the various composition fingerprint graphs in the codonopsis pilosula as a wholely to treat, pay attention to each front and back that constitute fingerprint characteristic peak order and mutual relation, pay attention to whole facial feature, avoided judging the one-sidedness of FUFANG DANSHEN DIWAN total quality, for quality complete, that accurately estimate codonopsis pilosula provides new reference standard because of only measuring one, two chemical constituent.
The present invention has that method is easy, stable, precision is high, favorable reproducibility, the characteristics that are easy to grasp.
Stability test (S1～S5 represents same batch 5 parts in sample respectively)
| Similarity | S1 | S2 | S3 | S4 | S5 | Reference fingerprint |
| S1 S2 S3 S4 | 1 0.999 0.998 0.996 | 0.999 1 0.999 0.996 | 0.998 0.999 1 0.996 | 0.996 0.996 0.996 1 | 0.992 0.991 0.992 0.994 | 0.998 0.999 0.999 0.997 |
| The S5 reference fingerprint | 0.992 0.998 | 0.991 0.999 | 0.992 0.999 | 0.994 0.997 | 1 0.993 | 0.993 1 |
Precision test (S1～S5 represents same batch 5 parts in sample respectively)
| S1 | S2 | S3 | S4 | S5 | Reference fingerprint | |
| S1 S2 S3 S4 S5 reference fingerprint | 1 0.999 0.997 0.995 0.983 0.998 | 0.999 1 0.998 0.995 0.982 0.998 | 0.997 0.998 1 0.998 0.982 0.998 | 0.995 0.995 0.998 1 0.983 0.998 | 0.983 0.982 0.982 0.983 1 0.984 | 0.998 0.998 0.998 0.998 0.984 1 |
Repeatability test (S1～S5 represents same batch 5 parts in sample respectively)
| S1 | S2 | S3 | S4 | S5 | Reference fingerprint | |
| S1 S2 S3 S4 S5 reference fingerprint | 1 0.999 0.998 0.996 0.992 0.998 | 0.999 1 0.999 0.996 0.991 0.999 | 0.998 0.999 1 0.996 0.992 0.999 | 0.996 0.996 0.996 1 0.994 0.997 | 0.992 0.991 0.992 0.994 1 0.993 | 0.998 0.999 0.999 0.997 0.993 1 |
To those skilled in the art, technology contents disclosed according to the present invention, those skilled in the art will very clear other embodiment of the present invention, and the embodiment of the invention is only as example.Under the situation of not violating purport of the present invention and scope, can carry out various changes and improvements to the present invention.For example, use the different measurement result that detecting instrument obtained possibilities different, but as long as use method of quality control of the present invention, all within protection domain of the present invention.
Description of drawings
The finger printing of codonopsis pilosula
The specific embodiment
(1) assay method
According to high performance liquid chromatography (" appendix VID of Chinese pharmacopoeia version in 2000), measure in conjunction with the requirement of finger printing.
Instrument and reagent Waters 600E pump, Waters 996PDA detector, M
32Work station, 717 automatic samplers.Ultrasonic washing instrument (Ningbo Ke Sheng instrument plant, power 250W).
Acetonitrile (chromatographically pure, the U.S. produces); The Mi Libo ultra-pure water; Other solvent is an analytical pure.
Chromatographic condition and system suitability test Diamonsil C
18Post (Di Ma company), granularity 5 μ m, volume 250 * 4.6mm; Mobile phase: acetonitrile-0.05% phosphoric acid linear gradient elution (seeing the following form); 30 ℃ of column temperatures; Flow velocity 1.0ml/min; The detection wavelength is 203nm; Writing time: 100 minutes.Number of theoretical plate calculates by maximum peak and is not less than 3000.Integral condition: minimum peak height: 0; Smallest peaks area: 0; Threshold value: 250; Minimum half-peak breadth: 30.And forbade integration before 7 minutes.
The linear gradient elution proportion of mobile phase changes program
| Time (min) | Flow velocity (ml/min) | Acetonitrile (%) | 0.05% phosphoric acid (%) |
| 0 30 45 65 100 | 1.0 1.0 1.0 1.0 1.0 | 15 25 40 80 80 | 85 75 60 20 20 |
The accurate need testing solution 20 μ l that draw of algoscopy inject chromatograph of liquid, measure.The test sample finger printing compares with finger printing with contrast, and machine simulation similarity software for calculation " chromatographic fingerprints of Chinese materia medica similarity evaluation system " (Chinese Pharmacopoeia can be published, version 2 004A) calculates similarity as calculated.
Detect the selection of wavelength
According to aforementioned chromatographic condition, the accurate need testing solution sample introduction of drawing, in the 200nm-400nm wave-length coverage, measure, 203nm, the 210nm, 220nm, 230nm, 250nm, 270nm, 290nm, 310nm, 330nm, the 360nm chromatogram that show this product, 220nm, 230nm, 250nm, 270nm, 290nm, 310nm, 330nm, the sensitivity of 360nm chromatogram are low, the peak number order is few, fingerprint is poor, under 200nm～210nm wavelength, the baseline of chromatogram is flat, separates better, and peak shape is beautiful, comparatively speaking, 203nm is better.
Finger printing has not had chromatographic peak and has occurred from 2 hours chromatograms as can be known after 100 minutes, therefore 100 minutes collection of illustrative plates can reflect the main component of this product.
It batch is the sample of 040517-4 that precision test is got, prepare test sample by the need testing solution preparation method, continuous sample introduction 5 times, detect fingerprint image, as the reference template, time window is 1.0 with the 1st figure, with several peaks of 46,84,94 minutes as the multiple spot calibration peak, automatically after the coupling, result shows that the similarity of each finger printing all greater than 0.90, meets the requirement of fingerprint image.
Stability test is got the sample that lot number is 040517-4, prepares test sample by the need testing solution preparation method, respectively 0,9, detected fingerprint image in 19,48,60 hours, with the 1st figure as the reference template, time window is 1.0, with several peaks of 46,84,94 minutes as the multiple spot calibration peak, automatically after the coupling, result shows that the similarity of each finger printing all greater than 0.90, meets the requirement of fingerprint image.
5 parts in batch sample that is 040517-4 is got in the repeatability test, prepare test sample by the need testing solution preparation method, detect fingerprint image, with the template of the 1st figure as the spectrum peak match, time window is 1.0, with several peaks of 46,84,94 minutes as the multiple spot calibration peak, automatically after the coupling, result shows that the similarity of each finger printing all greater than 0.90, meets the requirement of fingerprint image.
Five, the foundation of chromatographic fingerprinting and identification
Computer simulation similarity software for calculation " chromatographic fingerprints of Chinese materia medica similarity evaluation system " selects for use: " the chromatographic fingerprints of Chinese materia medica similarity evaluation system " that selects the Chinese Pharmacopoeia meeting for use.
The foundation of chromatographic fingerprinting and identification are according to preceding method, the sample of ten batches of medical materials of this product is carried out the mensuration of finger printing, with the 1st batch of collection of illustrative plates as the reference template, time window is 1.0, with several peaks of 46,84,94 minutes as the multiple spot calibration peak, automatically after the coupling, the similarity that result shows each finger printing all between 0.90-1.00, meets the requirement of fingerprint image.
Claims (10)
1, a kind of method of quality control of codonopsis pilosula is characterized in that comprising the steps:
(a). the foundation of Radix Codonopsis reference fingerprint
The preparation of codonopsis pilosula control sample solution:
Take by weighing the medical material fine powder, add methanol solution, supersound process is put coldly, shakes up, and 0.45 μ m microporous filter membrane filters;
The mensuration of codonopsis pilosula reference fingerprint:
Draw above-mentioned control sample solution and inject chromatograph of liquid, use high performance liquid chromatography to measure, obtain the codonopsis pilosula reference fingerprint, chromatographic condition: chromatographic column is filler with the octadecylsilane chemically bonded silica; Adopt gradient elution, mobile phase is the acetonitrile phosphate aqueous solution; Detect wavelength 200～210nm;
(b). the mensuration of codonopsis pilosula product fingerprint collection of illustrative plates to be measured:
Get codonopsis pilosula product to be measured, measure the finger printing of this product to be measured according to Step By Condition described in above-mentioned (a);
(c). described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are compared, discern the quantity of the two common absworption peak that has, to determine whether product quality is qualified.
2, the method for quality control of codonopsis pilosula as claimed in claim 1 is characterized in that: the flow velocity 0.500～1.500ml/min of mobile phase in described (a) step, detect wavelength 200～205nm, 20～40 ℃ of column temperatures.
3, the method for quality control of codonopsis pilosula as claimed in claim 1 is characterized in that:
The preparation method of codonopsis pilosula control sample solution is in described (a) step, precision takes by weighing the medical material fine powder, puts in the tool plug triangular flask, the accurate methanol solution that adds, the accurate title, decide, supersound process 30 minutes is put coldly, claims to decide weight again, add methanol and supply the weight that subtracts mistake, shake up, 0.45 μ m microporous filter membrane filters, and gets subsequent filtrate as need testing solution;
Chromatographic condition: mobile phase A is 0.02% phosphate aqueous solution; Mobile phase B is the phosphate aqueous solution of 80% acetonitrile 0.02%;
The gradient elution program is as shown in the table:
。
4, method of quality control as claimed in claim 3 is characterized in that, 11 absworption peaks are arranged in the described reference fingerprint, and the absworption peak that wherein unimodal area surpasses total peak area 10% has 2, is respectively:
No. 10 peaks, average retention time RT is 84.452min, and RSD is 0%, and peak area is 1275.39, and RSD is 3%;
No. 11 peaks, average retention time RT is 94.316min, and RSD is 0%, and peak area is 3319.528, and RSD is 3.42%.
5, method of quality control as claimed in claim 4 is characterized in that, 11 absworption peaks are arranged in the described reference fingerprint, and the absworption peak that wherein unimodal area surpasses total peak area 5% has 4, is respectively:
No. 2 peaks, average retention time RT is 12.068min, and RSD is 0.53%, and peak area is 381.699, and RSD is 11.9%;
No. 3 peaks, average retention time RT is 12.711min, and RSD is 0.4%, and peak area is 634.231, and RSD is 14.32%;
No. 10 peaks, average retention time RT is 84.452min, and RSD is 0%, and peak area is 1275.39, and RSD is 3%;
No. 11 peaks, average retention time RT is 94.316min, and RSD is 0%, and peak area is 3319.528, and RSD is 3.42%.
6, method of quality control as claimed in claim 5 is characterized in that, 11 absworption peaks are arranged in the described reference fingerprint, and the absworption peak that wherein unimodal area surpasses total peak area 2% has 11, is respectively:
No. 1 peak, average retention time RT is 7.308, and RSD is 0.34, and peak area is 289.896, and RSD is 8.94
No. 2 peaks, average retention time RT is 12.068, and RSD is 0.53, and peak area is 381.699, and RSD is 11.9
No. 3 peaks, average retention time RT is 12.711, and RSD is 0.4, and peak area is 634.231, and RSD is 14.32
No. 4 peaks, average retention time RT is 14.148, and RSD is 0.32, and peak area is 355.482, and RSD is 2.2
No. 5 peaks, average retention time RT is 21.677, and RSD is 0.35, and peak area is 270.857, and RSD is 35.46
No. 6 peaks, average retention time RT is 35.544, and RSD is 0.17, and peak area is 227.295, and RSD is 25.29
No. 7 peaks, average retention time RT is 36.097, and RSD is 0.21, and peak area is 293.056, and RSD is 10.03
No. 8 peaks, average retention time RT is 39.207, and RSD is 0.17, and peak area is 228.713, and RSD is 2.74
No. 9 peaks, average retention time RT is 45.348, and RSD is 0, and peak area is 282.564, and RSD is 4.46
No. 10 peaks, average retention time RT is 84.452, and RSD is 0, and peak area is 1275.39, and RSD is 3
No. 11 peaks, average retention time RT is 94.316, and RSD is 0, and peak area is 3391.528, and RSD is 3.42.
7, as the method for quality control of claim 4,5 or 6 described codonopsis pilosulas, it is characterized in that, described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are relatively, when the two finger printing has absworption peak identical more than 3 or 3, think the up-to-standard of described codonopsis pilosula product to be measured.
8, the method for quality control of codonopsis pilosula as claimed in claim 7, it is characterized in that, described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are relatively, when the two finger printing has absworption peak identical more than 5 or 5, think the up-to-standard of described codonopsis pilosula product to be measured.
9, the method for quality control of codonopsis pilosula as claimed in claim 8, it is characterized in that, described codonopsis pilosula product fingerprint collection of illustrative plates to be measured and described codonopsis pilosula reference fingerprint are relatively, when the two finger printing has absworption peak identical more than 7 or 7, think the up-to-standard of described codonopsis pilosula product to be measured.
10, codonopsis pilosula method of quality control as claimed in claim 9, it is characterized in that tested codonopsis pilosula product fingerprint collection of illustrative plates and contrast codonopsis pilosula finger printing compare, when the two finger printing has 8 identical absworption peaks, think the up-to-standard of described codonopsis pilosula product to be measured.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105403674A (en) * | 2015-12-11 | 2016-03-16 | 陇西保和堂药业有限责任公司 | Codonopsis pilosula sheet quality control method |
| CN107064319A (en) * | 2016-09-27 | 2017-08-18 | 贵阳中医学院 | The assay method of Guizhou codonopsis pilosula HPLC characteristic spectrums |
| CN109061024A (en) * | 2018-10-17 | 2018-12-21 | 湖南时代阳光药业股份有限公司 | The construction method of the standard finger-print of child speen supporting particle and its application |
| CN109613154A (en) * | 2018-12-18 | 2019-04-12 | 贵阳中医学院 | A kind of method for building up of the peaceful codonopsis pilosula HPLC characteristic spectrum of prestige |
| CN110243972A (en) * | 2019-07-04 | 2019-09-17 | 无限极（中国）有限公司 | Radix Codonopsis quality determining method based on spectrum effect relationship |
| CN114324666A (en) * | 2021-12-31 | 2022-04-12 | 成都中医药大学 | HPLC fingerprint detection method of Tibetan radix codonopsis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1351698B1 (en) * | 2000-12-22 | 2013-01-23 | Tasly Pharmaceutical Group Co., Ltd. | Herbal composition for angina pectoris, method to prepare same and uses thereof |
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2006
- 2006-06-08 CN CN 200610014203 patent/CN101084974B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105403674A (en) * | 2015-12-11 | 2016-03-16 | 陇西保和堂药业有限责任公司 | Codonopsis pilosula sheet quality control method |
| CN107064319A (en) * | 2016-09-27 | 2017-08-18 | 贵阳中医学院 | The assay method of Guizhou codonopsis pilosula HPLC characteristic spectrums |
| CN107064319B (en) * | 2016-09-27 | 2019-07-02 | 贵阳中医学院 | The measuring method of Guizhou codonopsis pilosula HPLC characteristic spectrum |
| CN109061024A (en) * | 2018-10-17 | 2018-12-21 | 湖南时代阳光药业股份有限公司 | The construction method of the standard finger-print of child speen supporting particle and its application |
| CN109613154A (en) * | 2018-12-18 | 2019-04-12 | 贵阳中医学院 | A kind of method for building up of the peaceful codonopsis pilosula HPLC characteristic spectrum of prestige |
| CN110243972A (en) * | 2019-07-04 | 2019-09-17 | 无限极（中国）有限公司 | Radix Codonopsis quality determining method based on spectrum effect relationship |
| CN114324666A (en) * | 2021-12-31 | 2022-04-12 | 成都中医药大学 | HPLC fingerprint detection method of Tibetan radix codonopsis |
| CN114324666B (en) * | 2021-12-31 | 2023-11-24 | 成都中医药大学 | HPLC fingerprint detection method for Tibetan radix codonopsis |
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