CN101757050A - Method for measuring total ginkgolic acid in ginkgo biloba extract - Google Patents
Method for measuring total ginkgolic acid in ginkgo biloba extract Download PDFInfo
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Abstract
The invention discloses a method for measuring total ginkgolic acid in a ginkgo biloba extract. The method comprises the following steps: the ginkgo biloba extract is dissolved and dispersed by alcohol in an ultrasonic manner, and is extracted by petroleum ether; the petroleum ether is collected; a solvent is recovered until and the extract is dried; methanol is added until the volume is constant, and then the measuring is finished. By adopting the ultrasonic extraction method, the invention can enhance the penetrating power of the solvent against medicinal powder by utilizing the cavitation effect, mechanical effect and thermal effect of ultrasonic waves, so as to increase the dissolving and dispersing speed of the extract, thereby not only being beneficial for uniform dissolving-out and dispersing of the total ginkgolic acid in the ginkgo biloba extract, but also improving the analyzing accuracy for a sticky and caked sample which is poor in physical properties and contains many insoluble components. The invention also has the following characteristics: the sampling quantity is small and the operation is convenient; the sample is not directly dissolved in methanol or petroleum ester but is extracted by petroleum ester, thereby removing partial impurities and protecting columns for HPLC analysis.
Description
Technical field
The present invention relates to the assay method of total ginkgolic acids in a kind of Semen Ginkgo extrac.
Background technology
Semen Ginkgo (Ginkgo Biloba) is the most ancient a kind of in the existing seed plant, also is unique survivor of the Semen Ginkgo family when tracking to more than 200,000,000 years the first two century.In China, ginkgo agent has been used for the treatment of existing more than 5000 year, that is to say, just begins to be used for the treatment of from the origin the earliest of Chinese herbal medicine.Since the sixties, the plant extract of Folium Ginkgo is used for the treatment of cerebrovascular and peripheral vascular disease in a lot of countries, as Germany, France, Japan and Korea S.
The main component of Folium Ginkgo is a flavone compound, it comprises at least 14 kinds of different chemical compounds, flavonol for example, flavone, flavanone and bisflavone or the like, in these chemical compounds, flavonoid glycoside and flavonol glycosides comprise kaempferide, Quercetin and isorhamnetin and glucose or rhamnose conjugate, be the Semen Ginkgo extrac (Tebonin that is used for the treatment of purpose on the market, Tanakan, Roekan or EGb761) in the most significant part, experiment shows that flavonoid glycoside and flavonol glycosides are strong antioxidant, thereby can remove oxygen-derived free radicals and avoid the cell and the tissue injury that cause owing to age ageing, they can have a strong impact on brain function, comprise memory and attention.Flavonoid glycoside and flavonol glycosides also can improve the peripheral vessels circulation in addition.
In addition, outside the apricot yellow ketone of desilver, another active component is obedient lactone in the Folium Ginkgo, comprises ginkalide A, B, C, J, M and bilobalide, and bilobalide is the obedient class that contains lactone structure.Recent findings is because the characteristic of their antagonism platelet activating factor (PAF), and ginkalide A, B, C, M particularly bilobalide can treat platelet activating factor effectively and induce an illness as asthma, bronchitis, alzheimer disease, allergy, cardiac function imbalance, rheumatism or the like and other blood circulation diseases.
Except that above-claimed cpd, Folium Ginkgo contains 12 kinds of alkyl phenol acid compounds at least, comprises ginkgoic acid, and it is the 6-alkyl salicylate, and alkyl is positive 13 carbon have 0-3 two keys to positive 19 carbon a alkyl group.Ginkgoic acid is the factor of Semen Ginkgo extrac toxic action, and these toxic actions comprise gastrointestinal side effect, headache, skin irritation and edema.Since the sixties, anaphylactoid report has appearred behind existing contacted Folium Ginkgo of many cases and the fruit, therefore to the accurate mensuration of gingkolic acid to the safety of Semen Ginkgo class preparation, especially injection very important that seem.
" to the total ginkgolic acids assay, in the preparation of need testing solution, directly get the about 10g of Semen Ginkgo extrac powder in the Chinese pharmacopoeia 2005 editions, the accurate title, decide, put in the tool plug conical flask, accurate petroleum ether (60～90 ℃) 50ml that adds claims to decide weight, reflux, extract, 2 hours, put coldly, claim again to decide weight, supply the weight that subtracts mistake with petroleum ether (60～90 ℃), shake up, filter.Precision is measured subsequent filtrate 25ml, and decompression and solvent recovery is to doing, and precision adds methanol 2ml, and close plug shakes up, promptly.Chromatographiccondition: chromatographic column is that octadecyl silane is a filler, and mobile phase is methanol-1% glacial acetic acid (90: 10); Flow velocity 1mL/min, the detection wavelength is 310nm.This law sampling amount is big, and the influence that measurement result is subjected to the sample character is bigger,, if the sample moisture absorption, being clamminess the result who measures can be obviously on the low side, return time is long.
The paper " content of ginkgoic acid in high effective liquid chromatography for measuring Folium Ginkgo extract and the preparation thereof " of " Acta Pharmaceutica Sinica " 2003 the 38th volumes o. 11th, reported that Folium Ginkgo extract and preparation thereof are through Petroleum ether extraction, extracting solution concentrates back petroleum ether standardize solution, HPLC directly measures. and chromatographiccondition: chromatographic column is Inertsil ODS-2, mobile phase is methanol-3% glacial acetic acid solution (92: 8), flow velocity 1.0mL/min, 40 ℃ of column temperatures, ultraviolet detection wavelength 310nm.
The paper " spectrophotometry of ginkgoic acid " of " analytical chemistry research notes " 2004 the 32nd the 5th phases of volume, reported that the ginkgoic acid crude extract is after a preliminary clearning of silica gel column chromatography is handled, spectrophotography is surveyed ginkgoic acid at 242nm or 310nm wavelength, and is suitable with the result who measures with the HPLC method at 310nm wavelength place.Chromatographiccondition: chromatographic column is Inertsil ODS-2, and mobile phase is methanol-3% glacial acetic acid solution (92: 8), flow velocity 1.0mL/min, 40 ℃ of column temperatures, ultraviolet detection wavelength 310nm.Need to make the standard solution of several concentration therebetween, measure absorbance and do standard curve; Sample is handled through a preliminary clearning of silica gel column chromatography; The mensuration of monomeric preparation of ginkgoic acid and specific absorbance, the operation more complicated.
Because existing document lacks systematic research to the extraction and determination of Semen Ginkgo extrac total ginkgolic acids, and the extracting method of analytic sample preparation also exists, and sample volume is big, the extraction shortcoming that return time is long, measurement result is on the low side, so be necessary to seek the method for total ginkgolic acids in more effective mensuration Semen Ginkgo extrac.
Summary of the invention
The objective of the invention is to overcome the deficiency that prior art exists, provide a kind of sampling amount little, be uniformly dispersed, more accurately the assay method of total ginkgolic acids in the Semen Ginkgo extrac.
To achieve these goals, the present invention adopts following technical scheme:
A kind of method of measuring total ginkgolic acids in the Semen Ginkgo extrac may further comprise the steps:
A, get Semen Ginkgo eo-acid reference substance, add methanol and make the quantitative reference substance solution of using; Other gets the total ginkgolic acids reference substance, adds methanol and makes the location reference substance solution;
B, Semen Ginkgo extrac add alcoholic solution, and be ultrasonic, and alcoholic solution extracts with alkane solvents, collects alkane solvents, and decompression and solvent recovery adds methanol constant volume to doing, and promptly gets need testing solution;
C, according to high effective liquid chromatography for measuring, the pillar of octadecyl silane filler, methanol: 1% glacial acetic acid solution=90: 10 is a mobile phase, and the detection wavelength is 310nm, flow velocity 1ml/min.
Get reference substance solution and need testing solution sample introduction respectively, obtain chromatogram;
D, calculate in the need testing solution total peak area with the corresponding chromatographic peak of total ginkgolic acids reference substance,
Calculate total ginkgolic acids content with Semen Ginkgo eo-acid reference substance external standard method, promptly.
In the method for total ginkgolic acids, the described alcoholic solution of step b is preferably methanol solution in the said determination Semen Ginkgo extrac.The present invention has adopted alcohol that Semen Ginkgo extrac is dissolved, preferably methanol solution in the sample preparation process, sample is dissolved, can avoid solid sample to carry out being wrapped in the solid owing to the phenolic acid composition in the mensuration process like this, what cause easily is inhomogeneous, makes measurement result on the low side.
In the method for total ginkgolic acids, the volumetric concentration of the described methanol solution of step b is preferably 20～80% in the said determination Semen Ginkgo extrac.The emulsion that causes when can avoid alkane solvent to extract, layering is rapid, and the bed boundary is clear; Also avoided the miscible of 2 phases, saved the operating time and made things convenient for the operation that extracts.Optimal volume concentration is 70%.
In the method for total ginkgolic acids, Semen Ginkgo extrac and alcoholic solution volume ratio are 1: 6～1: 25 among the step b, are preferably 1: 18 in the said determination Semen Ginkgo extrac.
In the method for total ginkgolic acids, ultrasonic time is preferred 20～60min among the step b in the said determination Semen Ginkgo extrac.
In the method for total ginkgolic acids, alkane solvents is preferably petroleum ether described in the step b in the said determination Semen Ginkgo extrac.
In the said determination Semen Ginkgo extrac in the method for total ginkgolic acids, ultrasonic back methanol solution helps fully total ginkgolic acids to be extracted with the extraction of the alkane solvents of 1: 1 volume 4～6 times among the step b, has improved the accuracy of analysis.The number of times of preferred extraction is 5 times.
Compared with prior art, the present invention has following beneficial effect:
1, in the sample preparation process, adopted the method for supersound extraction, utilize hyperacoustic cavitation effect, mechanical effect and heat effect etc. to strengthen the penetration power of solvent to medicated powder, the speed that has increased the extract dissolving and loose, help the stripping of total ginkgolic acids in the Semen Ginkgo extrac and be uniformly dispersed, bad for physical behavior, thickness, caking, the sample that the indissoluble composition is many also can improve the accuracy of analyzing.
2, the sampling amount of detection method of the present invention is 5g, has reduced sampling amount, and experimental implementation is more convenient.
3, the sample of detection method of the present invention is avoided directly with methanol or petroleum ether dissolution, but adopts petroleum ether extraction, has dispeled partial impurities, has protected HPLC to analyze and has used pillar.
Further set forth technical scheme of the present invention below by specific embodiment
The specific embodiment
Sample is a Semen Ginkgo extrac, and total ginkgolic acids content is 705ppm.
Comparative Examples 1: implement " the mensuration scheme of total ginkgolic acids in the Chinese pharmacopoeia version Semen Ginkgo extrac in 2005: in the preparation of need testing solution, directly get the about 10g of Semen Ginkgo extrac powder, the accurate title, decide, and puts in the tool plug conical flask, accurate petroleum ether (60～90 ℃) 50ml that adds, claim to decide weight, reflux, extract, 2 hours is put cold, claim to decide weight again, supply the weight that subtracts mistake with petroleum ether (60～90 ℃), shake up, filter.Precision is measured subsequent filtrate 25ml, and decompression and solvent recovery is to doing, and precision adds methanol 2ml, and close plug shakes up, promptly.Chromatographiccondition: chromatographic column is that octadecyl silane is a filler, and mobile phase is methanol-1% glacial acetic acid (90: 10); Flow velocity 1mL/min, the detection wavelength is 310nm.
Table 1, by " Chinese pharmacopoeia 2005 editions is measured the total ginkgolic acids result
The result shows: as stated above, parallel getting after 5 duplicate samples handle, it is respectively 613ppm, 541ppm, 452ppm, 597ppm, 604ppm that filtrate records the total ginkgolic acids result, and residue passes through reflow treatment again, all contain total ginkgolic acids, and filtrate mensuration total ginkgolic acids result's RSD% is 12.0%.Show with this method sample volume greatly, the reflow treatment time is long, and the sample total ginkgolic acids disperses inhomogeneous, causes the RSD% of measurement result to reach 12.0%, and result's accuracy is not high.
Embodiment 2: the mensuration scheme of implementing total ginkgolic acids in the Semen Ginkgo extrac of the present invention:
Get the about 5g of Semen Ginkgo extrac powder, the accurate title, decide, add 40% methanol solution 50ml, promptly Semen Ginkgo extrac and solvent ratios are 1: 10, behind the supersound process 20min, methanol solution is with the petroleum ether extraction 3 times of 1: 1 amount, collect petroleum ether liquid, decompression and solvent recovery is to doing, and precision adds dissolve with methanol, be settled to 5ml, promptly get need testing solution.The octadecyl silane post, methanol: 1% glacial acetic acid solution=90: 10 is a mobile phase, and the detection wavelength is 310nm, flow velocity 1ml/min.Get reference substance solution and need testing solution sample introduction respectively, obtain chromatogram.Calculate total ginkgolic acids content with external standard method.Parallel 3 duplicate samples of getting are handled, and record total ginkgolic acids 581ppm, and RSD% is 1.7%.
Embodiment 3: shown in embodiment 2, different is: add 60% methanol solution, and supersound process 20min, methanol solution is with the petroleum ether extraction 4 times of 1: 1 amount.Parallel 3 duplicate samples of getting are handled, and record total ginkgolic acids 620ppm, and RSD% is 1.4%.Show along with ultrasonic solvent strength be elevated to 60% and extraction times mention 4 times, measuring total ginkgolic acids content has increase.
Embodiment 4: shown in embodiment 2, different is: 70% methanol solution, and supersound process 30min, methanol solution are with the petroleum ether extraction 4 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, and record total ginkgolic acids 677ppm, and RSD% is 1.4%.Along with the increase of ultrasonic time, the content of total ginkgolic acids also has increase.
Embodiment 5: shown in embodiment 2, different is: 70% methanol solution, and supersound process 30min, methanol solution are with the petroleum ether extraction 5 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, and record total ginkgolic acids 697ppm, and RSD% is 0.98%.Along with the increase of extraction times, the content of total ginkgolic acids also has increase.
Embodiment 6: shown in embodiment 2, different is: 80% methanol solution, and supersound process 30min, methanol solution are with the petroleum ether extraction 6 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, and record total ginkgolic acids 693ppm, and RSD% is 1.0%.Ultrasonic solvent strength is increased to 80%, and extraction times is increased to 6 times, and total ginkgolic acids has trace to descend on the contrary.
Embodiment 7: shown in embodiment 2, different is: add 70% methanol solution 70ml, be that Semen Ginkgo extrac and solvent ratios are 1: 14, supersound process 30min, methanol solution is with the petroleum ether extraction 5 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, record total ginkgolic acids 700ppm, RSD% is 0.4%.
Embodiment 8: shown in embodiment 2, different is: add 70% methanol solution 90ml, be that Semen Ginkgo extrac and solvent ratios are 1: 18, supersound process 30min, methanol solution is with the petroleum ether extraction 5 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, record total ginkgolic acids 705ppm, RSD% is 0.3%.
Embodiment 9: shown in embodiment 2, different is: add 70% methanol solution 125ml, be that Semen Ginkgo extrac and solvent ratios are 1: 25, supersound process 60min, methanol solution is with the petroleum ether extraction 5 times of 1: 1 amount, and parallel 3 duplicate samples of getting are handled, record total ginkgolic acids 689ppm, RSD% is 0.7%.The ultrasonic solubilizer 125ml of institute is too big, and general analysis is with separatory funnel maximum 250ml.Ultrasonic 60min, solution temperature have obvious rising, cause caking easily, influence the distribution of total ginkgolic acids.
Claims (10)
1. method of measuring total ginkgolic acids in the Semen Ginkgo extrac may further comprise the steps:
A, get Semen Ginkgo eo-acid reference substance, add methanol and make the quantitative reference substance solution of using; Other gets the total ginkgolic acids reference substance, adds methanol and makes the location reference substance solution;
B, Semen Ginkgo extrac add alcoholic solution, and be ultrasonic, and alcoholic solution extracts with alkane solvents, collects alkane solvents, and decompression and solvent recovery adds methanol constant volume to doing, and promptly gets need testing solution;
C, according to high effective liquid chromatography for measuring, the pillar of octadecyl silane filler, methanol: 1% glacial acetic acid solution=90: 10 is a mobile phase, and the detection wavelength is 310nm, flow velocity 1ml/min. gets reference substance solution and need testing solution difference sample introduction, obtains chromatogram;
D, calculate in the need testing solution total peak area with the corresponding chromatographic peak of total ginkgolic acids reference substance,
Calculate total ginkgolic acids content with Semen Ginkgo eo-acid reference substance external standard method.
2. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 1 is characterized in that the described alcoholic solution of step b is a methanol solution.
3. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 2, the volumetric concentration that it is characterized in that the described alcoholic solution of step b is 20～80%.
4. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 3, the volumetric concentration that it is characterized in that the described methanol solution of step b is 70%.
5. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 1 is characterized in that among the step b that Semen Ginkgo extrac and alcoholic solution volume ratio are 1: 6～1: 25.
6. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 5 is characterized in that among the step b that Semen Ginkgo extrac and alcoholic solution volume ratio are 1: 18.
7. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 1 is characterized in that among the step b that ultrasonic time is 20～60min.
8. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 1 is characterized in that among the step b that described alkane solvents is a petroleum ether.
9. according to the method for total ginkgolic acids in claim 1 or the 8 described mensuration Semen Ginkgo extrac, it is characterized in that among the step b that ultrasonic back methanol solution is with the extraction of the alkane solvents of 1: 1 volume 4～6 times.
10. the method for total ginkgolic acids in the mensuration Semen Ginkgo extrac according to claim 9, the number of times that it is characterized in that described extraction is 5 times.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2452944C1 (en) * | 2010-10-05 | 2012-06-10 | Государственное образовательное учреждение высшего профессионального образования "Самарский государственный университет" | Method of authenticating medicinal plant raw material and apparatus for realising said method |
| CN103163252A (en) * | 2012-04-23 | 2013-06-19 | 成都百裕科技制药有限公司 | Determination method for total ginkgolic acid in ginkgolide composition |
| CN104407082A (en) * | 2014-11-27 | 2015-03-11 | 中国科学院上海药物研究所 | Method for detecting alkylphenol compounds in ginkgo leaf raw material and preparation |
| CN107315058A (en) * | 2017-05-04 | 2017-11-03 | 黑龙江珍宝岛药业股份有限公司 | A kind of method of total ginkgoic acid in detection ginkgo biloba succi |
| CN111487345A (en) * | 2020-04-28 | 2020-08-04 | 劲牌持正堂药业有限公司 | Method for rapidly detecting total ginkgoic acid in sample in production process of ginkgo leaf extract |
-
2010
- 2010-02-23 CN CN2010101170783A patent/CN101757050B/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2452944C1 (en) * | 2010-10-05 | 2012-06-10 | Государственное образовательное учреждение высшего профессионального образования "Самарский государственный университет" | Method of authenticating medicinal plant raw material and apparatus for realising said method |
| CN103163252A (en) * | 2012-04-23 | 2013-06-19 | 成都百裕科技制药有限公司 | Determination method for total ginkgolic acid in ginkgolide composition |
| CN103163252B (en) * | 2012-04-23 | 2014-09-24 | 成都百裕科技制药有限公司 | Determination method for total ginkgolic acid in ginkgolide composition |
| CN104407082A (en) * | 2014-11-27 | 2015-03-11 | 中国科学院上海药物研究所 | Method for detecting alkylphenol compounds in ginkgo leaf raw material and preparation |
| CN104407082B (en) * | 2014-11-27 | 2016-03-09 | 中国科学院上海药物研究所 | The detection method of alkylphenol compounds in a kind of ginkgo leaf raw material and preparation |
| CN107315058A (en) * | 2017-05-04 | 2017-11-03 | 黑龙江珍宝岛药业股份有限公司 | A kind of method of total ginkgoic acid in detection ginkgo biloba succi |
| CN111487345A (en) * | 2020-04-28 | 2020-08-04 | 劲牌持正堂药业有限公司 | Method for rapidly detecting total ginkgoic acid in sample in production process of ginkgo leaf extract |
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Owner name: GUANGZHOU HANFANG PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: HANFANG MODEM CHINESE TRADITIONAL MEDICINE RESEARCH DEVELOPMENT CO., LTD., GUANG |
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