CN101078713B - Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard - Google Patents
Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard Download PDFInfo
- Publication number
- CN101078713B CN101078713B CN200710042984XA CN200710042984A CN101078713B CN 101078713 B CN101078713 B CN 101078713B CN 200710042984X A CN200710042984X A CN 200710042984XA CN 200710042984 A CN200710042984 A CN 200710042984A CN 101078713 B CN101078713 B CN 101078713B
- Authority
- CN
- China
- Prior art keywords
- finger
- gynostemma pentaphylla
- peak
- fingerprint
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000002956 Gynostemma pentaphyllum Nutrition 0.000 title claims description 55
- 238000001514 detection method Methods 0.000 title claims description 10
- 240000006509 Gynostemma pentaphyllum Species 0.000 title description 42
- 239000003814 drug Substances 0.000 title description 5
- 239000000463 material Substances 0.000 claims abstract description 43
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 17
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 24
- 230000014759 maintenance of location Effects 0.000 claims description 22
- 150000001875 compounds Chemical class 0.000 claims description 19
- 241001065361 Gynostemma Species 0.000 claims description 13
- 229930182494 ginsenoside Natural products 0.000 claims description 13
- 229940089161 ginsenoside Drugs 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000009826 distribution Methods 0.000 claims description 8
- 229930187479 gypenoside Natural products 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- ZRBFCAALKKNCJG-UHFFFAOYSA-N gypenoside-XVII Natural products C1CC(C2(CCC3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC(C(C(O)C1O)O)OC1COC1OC(CO)C(O)C(O)C1O ZRBFCAALKKNCJG-UHFFFAOYSA-N 0.000 claims description 7
- 239000002250 absorbent Substances 0.000 claims description 6
- 230000002745 absorbent Effects 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- WEVYAHXRMPXWCK-UHFFFAOYSA-N acetonitrile Substances CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000010829 isocratic elution Methods 0.000 claims description 5
- 239000012071 phase Substances 0.000 claims description 5
- 239000013558 reference substance Substances 0.000 claims description 5
- 239000007791 liquid phase Substances 0.000 claims description 4
- 230000003292 diminished effect Effects 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000012982 microporous membrane Substances 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- NODILNFGTFIURN-GZPRDHCNSA-N ginsenoside Rb2 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1OC[C@H](O)[C@H](O)[C@H]1O NODILNFGTFIURN-GZPRDHCNSA-N 0.000 claims description 2
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000003908 quality control method Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000012567 medical material Substances 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- 241000196324 Embryophyta Species 0.000 description 11
- 210000002615 epidermis Anatomy 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000002792 vascular Effects 0.000 description 4
- 229930186669 gynosaponin Natural products 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- OORMXZNMRWBSTK-LGFJJATJSA-N dammarane Chemical compound C1CCC(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@H]([C@H](C)CCCC(C)C)[C@H]4CC[C@@H]3[C@]21C OORMXZNMRWBSTK-LGFJJATJSA-N 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000013441 quality evaluation Methods 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FBFMBWCLBGQEBU-RXMALORBSA-N (2s,3r,4s,5s,6r)-2-[(2r,3r,4s,5s,6r)-2-[[(3s,5r,6s,8r,9r,10r,12r,13r,14r,17s)-3,12-dihydroxy-4,4,8,10,14-pentamethyl-17-[(2s)-6-methyl-2-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhept-5-en-2-yl]-2,3,5,6,7,9,11,12,13,15,16,17-dodecah Chemical class O([C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(C[C@@H]([C@H]4C(C)(C)[C@@H](O)CC[C@]4(C)[C@H]3C[C@H]2O)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)C)(C)CC1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O FBFMBWCLBGQEBU-RXMALORBSA-N 0.000 description 1
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000503182 Gynostemma 'burmanicum' Species 0.000 description 1
- 240000003185 Gynostemma laxum Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000134253 Lanka Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010039897 Sedation Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 206010044302 Tracheitis Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- NODILNFGTFIURN-USYOXQFSSA-N ginsenoside Rb3 Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@](C)(CCC=C(C)C)[C@@H]1[C@@H]2[C@@]([C@@]3(CC[C@H]4C(C)(C)[C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)CC[C@]4(C)[C@H]3C[C@H]2O)C)(C)CC1)O[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O NODILNFGTFIURN-USYOXQFSSA-N 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 150000008131 glucosides Chemical group 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Images
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200710042984XA CN101078713B (en) | 2007-06-28 | 2007-06-28 | Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200710042984XA CN101078713B (en) | 2007-06-28 | 2007-06-28 | Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101078713A CN101078713A (en) | 2007-11-28 |
CN101078713B true CN101078713B (en) | 2010-08-11 |
Family
ID=38906288
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200710042984XA Active CN101078713B (en) | 2007-06-28 | 2007-06-28 | Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101078713B (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101509885B (en) * | 2008-10-31 | 2012-03-28 | 齐齐哈尔医学院 | Quantitative analysis method capable of reducing analytical apparatus spectrogram relative deviation RSD |
CN103018382A (en) * | 2012-12-07 | 2013-04-03 | 南京中医药大学 | Detection method of fingerprint spectrum similarity |
CN108445126B (en) * | 2018-02-02 | 2020-08-14 | 广州白云山和记黄埔中药有限公司 | Detection method of gypenoside extract |
CN109061022A (en) * | 2018-08-21 | 2018-12-21 | 浙江金大康动物保健品有限公司 | A kind of oriental wormwood detoxification particles Content of Chlorogenic Acid and Gardenoside Simultaneous Determination method |
CN114428127A (en) * | 2020-10-29 | 2022-05-03 | 中国石油化工股份有限公司 | Method for identifying petroleum products |
CN112876528A (en) * | 2021-01-15 | 2021-06-01 | 吉林农业大学 | Method for obtaining ginsenoside |
CN114894922B (en) * | 2022-04-19 | 2023-04-28 | 北京康仁堂药业有限公司 | Detection and quality control method of gynostemma pentaphylla water extract |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1529162A (en) * | 2003-10-16 | 2004-09-15 | 中山大学 | Method for constituting finger-print pyrogram of white atracty lodes and its standard finger-print pyrogram |
CN1547009A (en) * | 2003-12-17 | 2004-11-17 | 中山大学 | Tibet gadol medicinal material HPLC finger print construction method and standard finger print thereof |
CN1595145A (en) * | 2004-07-15 | 2005-03-16 | 广州中一药业有限公司 | Method for determining fingerprint pattern of white atractylodes rhizome as a medicinal material |
CN1707260A (en) * | 2005-05-11 | 2005-12-14 | 沈阳药科大学 | Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint |
CN1760667A (en) * | 2004-10-15 | 2006-04-19 | 成都优他制药有限责任公司 | Method for controlling quality of simply single drug |
CN1776419A (en) * | 2005-11-18 | 2006-05-24 | 沈阳药科大学 | Senso-like efficient liquid-phase chromatograph finger print atlas identifying method |
-
2007
- 2007-06-28 CN CN200710042984XA patent/CN101078713B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1529162A (en) * | 2003-10-16 | 2004-09-15 | 中山大学 | Method for constituting finger-print pyrogram of white atracty lodes and its standard finger-print pyrogram |
CN1547009A (en) * | 2003-12-17 | 2004-11-17 | 中山大学 | Tibet gadol medicinal material HPLC finger print construction method and standard finger print thereof |
CN1595145A (en) * | 2004-07-15 | 2005-03-16 | 广州中一药业有限公司 | Method for determining fingerprint pattern of white atractylodes rhizome as a medicinal material |
CN1760667A (en) * | 2004-10-15 | 2006-04-19 | 成都优他制药有限责任公司 | Method for controlling quality of simply single drug |
CN1707260A (en) * | 2005-05-11 | 2005-12-14 | 沈阳药科大学 | Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint |
CN1776419A (en) * | 2005-11-18 | 2006-05-24 | 沈阳药科大学 | Senso-like efficient liquid-phase chromatograph finger print atlas identifying method |
Non-Patent Citations (1)
Title |
---|
卢金清,陈黎,肖波, 万威,詹晓莲,徐玉婷,田耀平.绞股蓝皂苷部位高效液相2蒸发光散射法检测的特征图谱.《中国医院药学杂志》.2007,第27卷(第6期),707-709. * |
Also Published As
Publication number | Publication date |
---|---|
CN101078713A (en) | 2007-11-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101078713B (en) | Fingerprint detection method of gynostemma pentaphylla medicine added with internal standard | |
CN102818861B (en) | Finger-print establishment method of Qingdu Anshen capsule | |
CN101991661B (en) | Method for detecting Chinese patent drug containing at least two of white paeony root, ginseng, salvia miltiorrhiza, sweet wormwood, liquorice and angelica sinensis | |
CN101979052A (en) | Method for establishing high-performance liquid chromatography (HPLC) fingerprint of Guangxi dendrobium candidum wall. ex lindl and standard fingerprint of Guangxi dendrobium candidum wall. ex lindl | |
CN101549021B (en) | Method for testing quality of perfoliate knotweed medicinal materials | |
CN101851261B (en) | Polygonum perfoliatum medicinal material, method for preparing reference substance of active constituents in preparation thereof as well as content determination method | |
CN102370891A (en) | Method for authenticating dendrobium officinale by using HPLC fingerprint | |
CN102488819B (en) | Preparing method for daylily flower extract | |
CN106370756B (en) | A kind of detection method of Chinese materia medica preparation that preventing and treating infectious bronchitis of chicken | |
CN103472148B (en) | Fingerprint spectrum detection method of Chinese medicine composition preparation | |
CN109239220B (en) | A kind of quality determining method of Yupingfeng Granules | |
CN107648369A (en) | A kind of clarification process and method of quality control of the pharmaceutical composition that there is treatment acne to act on | |
CN106442809B (en) | The construction method and its finger-print of common wedgelet fern herb finger-print | |
CN103344717B (en) | Method for establishing rhizoma bletillae high performance liquid chromatography (HPLC) fingerprint spectrum and standard fingerprint spectrum thereof | |
CN108169345A (en) | Anaesthetic radix gentiane dahuvicae fingerprint and quality evaluating method | |
CN102309531A (en) | Detection method of American ginseng fingerprint | |
CN102628838B (en) | Fujian herb cusia HPLC fingerprint construction method | |
CN103969355A (en) | Identification method for fingerprint spectrum of astragalus medicinal material | |
CN110501433A (en) | A kind of discrimination method of the callicarpa nudiflora medicinal material of different sources | |
CN103969356B (en) | A kind of discrimination method of the finger printing of red rooted salvia | |
CN106645489B (en) | Survey the methods for commenting method measurement common wedgelet fern herb ingredient using one more | |
CN100478685C (en) | Method for establishing pinellia ternata water-soluble fingerprint | |
CN103076403A (en) | Quality inspection method for capsule for treating lower urinary tract infection | |
CN112924600B (en) | Fingerprint spectrum establishment method of preparation for treating joint swelling and pain caused by rheumatic arthralgia | |
CN103454359B (en) | The quality determining method of bignose rhinacanthus branchlet and leaf medicinal material |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee |
Owner name: SHANGHAI MODERN TCM CO., LTD. Free format text: FORMER NAME: SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECHNOLOGY DEVELOPMENT CO., LTD. |
|
CP01 | Change in the name or title of a patent holder |
Address after: 200051 7F-C, 600 lane, Tian Shan Road, Shanghai, Changning District, 4 Patentee after: Shanghai Sundise Chinese Medicine Technology Development Co.,Ltd. Address before: 200051 7F-C, 600 lane, Tian Shan Road, Shanghai, Changning District, 4 Patentee before: Shanghai Sundise |
|
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: 200051 7F-C, 600 lane, Tian Shan Road, Shanghai, Changning District, 4 Patentee after: Shanghai Baiyang Pharmaceutical Co.,Ltd. Address before: 200051 7F-C, 600 lane, Tian Shan Road, Shanghai, Changning District, 4 Patentee before: Shanghai Sundise Chinese Medicine Technology Development Co.,Ltd. |
|
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: Room 3301-3302, No. 1027 Changning Road, Changning District, Shanghai, 200051 Patentee after: Shanghai Baiyang Pharmaceutical Co.,Ltd. Country or region after: China Address before: 200051 7F-C, 600 lane, Tian Shan Road, Shanghai, Changning District, 4 Patentee before: Shanghai Baiyang Pharmaceutical Co.,Ltd. Country or region before: China |