CN101748140A - Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene and encoded protein and application thereof - Google Patents
Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene and encoded protein and application thereof Download PDFInfo
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- CN101748140A CN101748140A CN200810044093A CN200810044093A CN101748140A CN 101748140 A CN101748140 A CN 101748140A CN 200810044093 A CN200810044093 A CN 200810044093A CN 200810044093 A CN200810044093 A CN 200810044093A CN 101748140 A CN101748140 A CN 101748140A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene and an encoded protein and an application thereof. The schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene comprises nucleotide sequences shown by SEQ ID No. 1-SEQ ID No. 3; and schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase comprises a protein with nucleotide sequences shown by SEQ ID No. 4-SEQ ID No. 6 or a protein with the same functions formed by replacing and deleting or inserting one or a plurality of amino acids. The schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase can be applied to schistosome vaccine preparation, medicament screening, anti-schistosoma antibody preparation and serodiagnosis; and the schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene can be applied to gene therapy.
Description
Technical field
The present invention relates to a kind of glyceraldehyde 3-phosphate dehydro-genase, particularly relate to a kind of gene, albumen and application of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase.
Background technology
Schistosomicide is caused that by bilharzial infection 76 countries are arranged in the world, and the sufferer about 1.5 hundred million.Morbific schistosomicide has 3 kinds, both Schistosoma japonicum (Schistosoma japonicum), Schistosoma mansoni (S.mansoni) and Schistosoma haematobium (S.haematobium).At China's popular is Schistosoma japonicum.The popular of schistosomicide all causes tremendous loss for people's health and Economic development, and therefore, prevention and cure of schistosomiasis, development schistosomicide reagent for clinical diagnosis and antischistosomal medicine have huge social and economic benefit.
Can there be a lot of years in Schistosoma japonicum as parasite and not be killed by host's immunologic mechanism in human body and animal body, relevant with its immune evasion ability.Its immune evasion mechanism has antigen mimicking, antigen camouflage and immunomodulatory.Wherein immunomodulatory refers to the immunity system that (mainly being to suppress) host can be regulated, be disturbed to bilharzial albumen, makes its immune attack usefulness that can not bring into normal play, thereby helps bilharzial existence.
(Excretory/Secretory protein ESP) is exactly the protein that a class can be brought into play immunoregulation effect to bilharzial drainage/secretion antigen.These albumen are from bilharzial epidermis and gut epithelium, or the drainage/secretory of specialization.Each worm phase of schistosomicide all produces this proteinoid.ESP is potential drug target spot and vaccine molecule, thereby can disturb bilharzial immune evasion mechanism enhancing host's immunity to kill function because suppress these albumen; ESP is a potential diagnosis molecule, because these albumen are free in host's the blood, can detect with existing means easily.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene, its encoded protein and application, and this glyceraldehyde 3-phosphate dehydro-genase is potential drug targets and diagnosis molecule.
For solving the problems of the technologies described above, the gene of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase of the present invention comprises: have the nucleotide sequence shown in SEQ ID NO.1～SEQ ID NO.3.
Nucleotide sequence coded albumen shown in the described SEQ ID NO.1 with sequence shown in the SEQ ID NO.4.
Nucleotide sequence coded albumen shown in the described SEQ ID NO.2 with sequence shown in the SEQ ID NO.5.
Nucleotide sequence coded albumen shown in the described SEQ ID NO.3 with sequence shown in the SEQ ID NO.6.
Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase of the present invention comprises:
Have the albumen of aminoacid sequence shown in the SEQ ID NO.4 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen with identical function that forms by this albumen, this albumen is the albumen with SEQ ID NO.1 sequence encoding;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.5 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen with identical function that forms by this albumen, this albumen is the albumen with SEQ ID NO.2 sequence encoding;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.6 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen with identical function that forms by this albumen, this albumen is the albumen with SEQ ID NO.3 sequence encoding.
Though glyceraldehyde 3-phosphate dehydro-genase is considered to composing type albumen, and discovers that glyceraldehyde 3-phosphate dehydro-genase is multifunctional protein in fact, can be positioned on the film, just plays a role as membranin.The GAPDH that finds in schistosomicide excretion albumen may be from bilharzial epidermis.In addition, glyceraldehyde 3-phosphate dehydro-genase also may be by specific secretory or emiocytosis, because be exactly that specific secretory cell secretes glyceraldehyde 3-phosphate dehydro-genase in host body fluids a kind of anaerobic parasitic protozoa Entamoeba histolytica.
The present invention is by utilizing the proteomics means, analyzed the moiety of ESP of the vitro extraction of Schistosoma japonicum, find glyceraldehyde 3-phosphate dehydro-genase (the Glyceraldehyde-3-phosphate dehydrogenase of Schistosoma japonicum, GAPDH) be one of the main component of its ESP, show that this albumen may play a significant role aspect bilharzial immunomodulatory, the immune evasion.In addition, by the blood of direct analysis schistosoma japonicum infection animal (rabbit), find that this glyceraldehyde 3-phosphate dehydro-genase also exists really in host's blood, therefore, glyceraldehyde 3-phosphate dehydro-genase is potential drug targets and diagnosis molecule.
Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase of the present invention can be applicable to prepare blood fluke vaccine as immunogen; As the potential drug target, be applied to screening chemistry and other kind medicines; As the specificity schistosome antigen, be applied to prepare schistosomicide antibody; Be applied to the serodiagnosis aspect; The encoding gene of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase, the application aspect gene therapy.
Embodiment
Embodiment proteic separation of 1 Schistosoma japonicum excretion and identification experiment
1). the preparation and the protein extraction of Schistosoma japonicum adult excretion product:
Schistosoma japonicum adult is separated the back in the infection animal body and is washed 2 times with 1 * PBS (phosphate buffered saline buffer), and about 800 adults suspend with 1ml PBS, and low-speed centrifugal (500rpm) is collected the supernatant sucking-off after 10 minutes, repeats the merging supernatant 10 times.With the albumen in the acetone precipitation supernatant, dissolution precipitation 50mM Tris-HCl (pH 8.3), 5mM EDTA, 1mM phenylmethylsulfonyl fluoride (PMSF), 0.5% sodium lauryl sulphate (SDS) and 0.5mM dithiothreitol (DTT) (DTT), 0.5%SDS, ultrasonic short molten.
2). the proteic enzymolysis process of Schistosoma japonicum excretion:
The excretion egg white mixture spends the night with the pancreatin enzymolysis with the iodacetyl ammonia treatment of 10mM DTT and 55mM, dilution back.
3). the on-line chromatograph of Schistosoma japonicum excretion proteolysis peptide section separates and mass spectrum is identified:
The peptide mixt that enzymolysis produces pumps 30 μ l with vacuum with liquor capacity.Afterwards, on the peptide mixt of each component sample to anti-phase catch post (C18,5 μ m,
300 μ m i.d * 5mm, LC Packings), desalination under the flow velocity of 20 μ l/min.This 75 μ m * 150mm C18 post (LC Packings) of catching post and an analysis usefulness is connected then, just under the flow velocity of 200nl/min, wash-out enters the QSTAR pulser i of the electric spray ion source that Protana NanoES is housed peptide mixt from this pillar.Agilent 1100 capillary liquid phase systems (Agilent Technologies) are used to provide mobile phase A (0.5% acetic acid/water) and Mobile phase B (0.5% acetate/acetonitrile), linear gradient be in 60 minutes 5%B to 50%B, 50%B is to stop 15 minutes at 90%B to 90%B then in 30 minutes.The PicoTip that connects a 10-μ m diameter by one stainless two logical (Valco Instrument) behind analytical column receives fog-spray nozzle (New Objective), adds 25003000 volts spray voltage, to obtain stable spraying.MS/MS tandem mass spectrum spectrogram comes record by collection (IDA) and the task-circulation enhancing that information relies on.Measure in the scanning at each, choose 3-6 signal the strongest, with the ion of 2 to 3 electric charges, smash with the rolling collision energy, with the information of acquisition tandem mass spectrum.
4). the proteic software search of Schistosoma japonicum excretion is identified:
The database of mass spectrum search usefulness comprises the Schistosoma japonicum protein sequence of downloading from public database NCBI from 11717 (time is on October 16th, 2008).Search software is MASCOT (http://www.matrixscience.com/, Matrix Science).Search parameter is: maximum leakage point of contact, 3; Monoisotopic; The peptide charge number ,+1/+2/+3; Modify halfcystine carbamoylmethylation and methionine oxidation.
After Search Results merges, carry out filtering screening: 1. to the search result list of each tandem mass spectrum spectrogram, only extract the result of ranking, other then abandon at first with following standard; If 2. in the peptide section that search obtains KR is arranged, KK, RK, sequences such as RR, then this peptide section abandons; 3. search for the peptide section that obtains, length is 8 reservations more than the amino acid, less than 8 amino acid whose abandoning; 4. repeat the peptide section got to, only calculate that wherein score value is the highest, these peptide section score values are added up, as proteic score; 5. proteic score surpasses 60, just thinks believable result.The low albumen that divides keeps after manual the inspection or abandons.Match host or bovine trypsin, keratic peptide section, then abandon.
Carry out the search of reverse protein sequence library simultaneously.Oppositely protein pool is with the counter-rotating of Schistosoma japonicum protein sequence, keep length, form constant.According to forward storehouse and the result that reverse library searching obtains, determine false positive rate.Method of calculation: false positive=2* (oppositely storehouse peptide hop count)/(forward storehouse peptide hop count+reverse storehouse peptide hop count).False positive is controlled at 0%.
The excretion albumen of embodiment 2 direct analysis Schistosoma japonicum from infection animal serum
1). schistosoma japonicum cercariae infects rabbit, collects and infects the 42nd day the animal serum in back.Form with the liquid chromatography-mass spectrography analysing protein behind the serum protein enzymolysis.The Protein Data Bank of mass-spectrometric data search Schistosoma japonicum is according to above-mentioned standard screening peptide section.The peptide section that obtains is removed the peptide section of any coupling with the genes database (comprising mRNA, EST, gene order-checking data etc.) of Blast search rabbit, and the peptide section that remains all is that schistosomicide is distinctive.Utilize the peptide section then, search obtains corresponding schistosomicide protein sequence.
2). the quantitative analysis of albumen abundance: (Spectra count SC), represents the abundance height with this to calculate the mass spectrum sum that each Identification of Fusion Protein obtains.
The proteic proteomics qualification result of the external excretion of embodiment 3 Schistosoma japonicum
Identify 110 schistosomicide albumen altogether.Wherein glyceraldehyde 3-phosphate dehydro-genase is one of higher albumen of relative abundance, and the SC sum is 28, and peptide section score summation is 1170, identifies this proteic 6 kinds of peptide sections (table 1) altogether, and the fraction of coverage of protein sequence is 20%.This explanation, glyceraldehyde 3-phosphate dehydro-genase is one of main protein ingredient in the Schistosoma japonicum adult excretion product.Adult is in host (as the people) body, and its excretion product must be discharged in host's the blood circulation system, therefore, detects the existence of schistosomicide excretion product, just can detect schistosomicide.And the high albumen of abundance in the excretion product, the easiest being detected, therefore, glyceraldehyde 3-phosphate dehydro-genase is suitable clinical diagnosis molecule, can utilize existing universal method such as ELISA to detect schistosomicide characteristic protein in patient's blood sample easily.
The mass spectrum qualification result in external excretion protein groups of table 1. Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase
Sequence table
＜110〉Research Center of Shanghai Human Genome
＜120〉Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase gene, proteins encoded and application
<130>NP-08-12794
<160>6
<170>PatentIn?version?3.3
<210>1
<211>1144
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>1
cggtcacgta?ctaaaatgtc?gaaggctaag?gttggtatca?atgggtttgg?tcgtattggg 60
agacttgtac?tccgtgcagc?ttttctcaaa?aataccgtgg?atatagttgc?cgtaaatgat 120
ccattcatcg?atctagagta?catggtgtac?atgataaaat?atgactccac?ccatggaaag 180
tttcaaggtg?atgtttcggt?tgagaatgga?aaacttaatg?tcaatggaag?gcttatatca 240
gtttactgcg?agagggatcc?attgaacata?ccatggaaca?aggatggtgc?tgagtatgtt 300
gtagagtcca?ctggagtctt?cactacaatt?gataaagctc?aagctcatat?taaaaacgat 360
cgggctaaaa?aagttataat?atcagctccg?tcggcagacg?cacccatgtt?tgttgttggt 420
gtgaatgaaa?agacttacga?caagtcaatg?tctgtggttt?cgaatgcatc?gtgcaccaca 480
aactgtctag?cacctctagc?taaagtcatt?aatgacaatt?ttgaaatagt?tgaaggcctt 540
atgactactg?tacactcatt?tacggctacg?caaaagaccg?ttgatggacc?atcttcaaaa 600
ctgtggagag?atggtcgtgg?ggcgtttcag?aatattattc?cagcctccac?tggtgctgca 660
aaggcagtgg?gcaaagtcat?ccctgcatta?aacggaaagt?tgacaggaat?ggctttccgg 720
gtgcctacag?cgaatgtttc?agtagttgac?ctgacatgca?ggttgggcaa?aggagctacc 780
tacgatcaaa?tcaaggctgt?gatcaaagca?gccgcaaatg?gaccattaaa?aggcatcttg 840
gaatatactg?aagatgaagt?tgtcagctca?gactttattg?gatgtaccag?ttcatccata 900
tttgatgcaa?aggctggaat?ctctctcaac?aacaatttcg?tgaaactggt?ttcatggtac 960
gacaatgaat?tcggctacag?ttgccgcgtg?gtcgatctca?tcacgcatat?gcatagagtc 1020
gaccattctt?aataaaatag?ttttcaacgt?agtctacgat?tttcgttcat?agataacgaa 1080
taaagtgttg?atctactcta?ttattttatc?attcttaacc?aaatatcatt?acaattcaac 1140
atgc 1144
<210>2
<211>1125
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>2
aaaatgtcga?aggctaaggt?tggtatcaat?gggtttggtc?gtattgggag?acttgtactc 60
cgtgcagctt?ttctcaaaaa?taccgtggat?atagttgccg?taaatgatcc?attcatcgat 120
ttagagtaca?tggtgtacat?gataaaatat?gactccaccc?atggaaagtt?tcaaggtgat 180
gtttcggttg?agaatggaaa?acttaatgtc?aatggaaggc?ttatatcagt?ttactgcgag 240
agggatccat?tgaacatacc?atggaacaag?gatggtgctg?agtatgttgt?agagtccact 300
ggagtcttca?ctacaattga?taaagctcaa?gctcatatta?aaaacgatcg?ggctaaaaaa 360
gttataatat?cagctccgtc?ggcagacgca?cccatgtttg?ttgttggtgt?gaatgaaaag 420
acttacgaca?agtcaatgtc?tgtggtttcg?aatgcatcgt?gcaccacaaa?ctgtctagca 480
cctctagcta?aagtcattaa?tgacaatttt?gaaatagttg?aaggccttat?gactactgta 540
cactcattta?cggctacgca?aaagaccgtt?gatggaccat?cttcaaaact?gtggagagat 600
ggtcgtgggg?cgtttcagaa?tattattcca?gcctccactg?gtgctgcaaa?ggcagtgggc 660
aaagtcatcc?ctgcattaaa?cggaaagttg?acaggaatgg?ctttccgggt?gcctacagcg 720
aatgtttcag?tagttgacct?gacatgcagg?ttgggcaaag?gagctaccta?cgatcaaatc 780
aaggctgtga?tcaaagcagc?cgcaaatgga?ccattaaaag?gcatcttgga?atatactgaa 840
gacgaagttg?tcagctcaga?ctttattgga?tgtaccagtt?catccatatt?tgatgcaaag 900
gctggaatct?ctctcaacaa?caatttcgtg?aaactggttt?catggtacga?caatgaattc 960
ggctacagtt?gccgcgtggt?cgatctcatc?acgcatatgc?atagagtcga?ccattcttaa 1020
taaaatagct?ttcaacgtag?tctacgattt?tcgtttatag?ataacgaata?aagtgttgat 1080
ctactctatt?attttatcat?tcttaaccaa?atatcattat?aattc 1125
<210>3
<211>1148
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>3
aaaatgtcga?aggctaaggt?tggtatcaat?gggtttggtc?gtattgggag?acttgtactc 60
cgtgcagctt?ttctcaaaaa?taccgtggat?atagttgccg?taaatgatcc?attcatcgat 120
ttagagtaca?tggtgtacat?gataaaatat?gactccaccc?atggaaagtt?tcaaggtgat 180
gtttcggtgg?agaatggaaa?acttaatgtc?aatggaaggc?ttatatcagt?ttactgcgag 240
agggatccat?tgaacatacc?atggaacaag?gatggtgctg?agtatgttgt?agagtccact 300
ggagtcttca?ctacaattga?taaagctcaa?gctcatatta?aaaacgatcg?ggctaaaaaa 360
gttattatat?cagctccgtc?ggcagacgca?cccatgtttg?ttgttggtgt?gaatgaaaag 420
acttacgacc?agtcaatgtc?tgtggtttcg?aatgcttcgt?gcaccactaa?ctgtctagca 480
cctctagcta?aagtcattaa?tgacaatttt?gaaatagttg?aaggccttat?gactactgta 540
cactcattta?cggctacgca?aaagaccgtt?gatggaccat?cttcaaaact?gtggagagat 600
ggtcgtgggg?cgtttcagaa?tattattcca?gcctccactg?gtgctgcaaa?ggcagtgggc 660
aaagtcatcc?ctgcattaaa?cggaaagttg?acaggaatgg?ctttccgggt?gcctacagcg 720
aatgtttcag?tagttgacct?gacatgcagg?ttgggcaaag?gagctaccta?cgatcaaatc 780
aaggctgtga?tcaaagcagg?cgcaaatgga?ccattaaaag?gcatcttgga?atatactgaa 840
gatgaagttg?tcagctcaga?ctttattgga?tgtaccagtt?catccatatt?tgatgcaaag 900
gctggaatct?ctctcaacaa?caatttcgtg?aaactggttt?catggtacga?caatgaattc 960
ggctacagtt?gccgcgtggt?cgatctcatc?acgcatatgc?atagagtcga?ccattcttaa 1020
taaaatagtt?ttcaacgtag?tctacgattt?tcgttcatag?ataacgaata?aagtgttgat 1080
ctactctatt?attttatcat?tcttaaccaa?atatcattac?aattcaacat?gccaaaaaaa 1140
aaaaaaaa 1148
<210>4
<211>338
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>4
MSKAKVGING?FGRIGRLVLR?AAFLKNTVDI?VAVNDPFIDL?EYMVYMIKYD?STHGKFQGDV 60
SVENGKLNVN?GRLISVYCER?DPLNIPWNKD?GAEYVVESTG?VFTTIDKAQA?HIKNDRAKKV 120
IISAPSADAP?MFVVGVNEKT?YDKSMSVVSN?ASCTTNCLAP?LAKVINDNFE?IVEGLMTTVH 180
SFTATQKTVD?GPSSKLWRDG?RGAFQNIIPA?STGAAKAVGK?VIPALNGKLT?GMAFRVPTAN 240
VSVVDLTCRL?GKGATYDQIK?AVIKAAANGP?LKGILEYTED?EVVSSDFIGC?TSSSIFDAKA 300
GISLNNNFVK?LVSWYDNEFG?YSCRVVDLIT?HMHRVDHS 338
<210>5
<211>338
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>5
MSKAKVGING?FGRIGRLVLR?AAFLKNTVDI?VAVNDPFIDL?EYMVYMIKYD?STHGKFQGDV 60
SVENGKLNVN?GRLISVYCER?DPLNIPWNKD?GAEYVVESTG?VFTTIDKAQA?HIKNDRAKKV 120
IISAPSADAP?MFVVGVNEKT?YDKSMSVVSN?ASCTTNCLAP?LAKVINDNFE?IVEGLMTTVH 180
SFTATQKTVD?GPSSKLWRDG?RGAFQNIIPA?STGAAKAVGK?VIPALNGKLT?GMAFRVPTAN 240
VSVVDLTCRL?GKGATYDQIK?AVIKAAANGP?LKGILEYTED?EVVSSDFIGC?TSSSIFDAKA 300
GISLNNNFVK?LVSWYDNEFG?YSCRVVDLIT?HMHRVDHS 338
<210>6
<211>338
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>6
MSKAKVGING?FGRIGRLVLR?AAFLKNTVDI?VAVNDPFIDL?EYMVYMIKYD?STHGKFQGDV 60
SVENGKLNVN?GRLISVYCER?DPLNIPWNKD?GAEYVVESTG?VFTTIDKAQA?HIKNDRAKKV 120
IISAPSADAP?MFVVGVNEKT?YDQSMSVVSN?ASCTTNCLAP?LAKVINDNFE?IVEGLMTTVH 180
SFTATQKTVD?GPSSKLWRDG?RGAFQNIIPA?STGAAKAVGK?VIPALNGKLT?GMAFRVPTAN 240
VSVVDLTCRL?GKGATYDQIK?AVIKAGANGP?LKGILEYTED?EVVSSDFIGC?TSSSIFDAKA 300
GISLNNNFVK?LVSWYDNEFG?YSCRVVDLIT?HMHRVDHS 338
Claims (8)
1. the gene of a Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase is characterized in that: comprising: have the nucleotide sequence shown in SEQ IDNO.1～SEQ ID NO.3.
2. the gene of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase as claimed in claim 1 is characterized in that: the albumen of sequence shown in the nucleotide sequence coded SEQ of the having ID NO.4 shown in the described SEQ ID NO.1; Nucleotide sequence coded albumen shown in the described SEQ ID NO.2 with sequence shown in the SEQ ID NO.5; Nucleotide sequence coded albumen shown in the described SEQ IDNO.3 with sequence shown in the SEQ ID NO.6.
3. Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase is characterized in that: comprising:
Have the albumen of aminoacid sequence shown in the SEQ ID NO.4 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.5 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.6 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen.
4. the application of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase aspect the preparation blood fluke vaccine.
5. the application of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase aspect screening of medicaments.
6. the application of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase aspect preparation schistosomicide antibody.
7. the application of Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase aspect serodiagnosis.
8. a Schistosoma japonica glyceraldehyde-3-phosphate dehydrogenase utilizes its encoding gene, the application aspect gene therapy.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667200A (en) * | 2012-09-06 | 2014-03-26 | 江苏省血吸虫病防治研究所 | Schistosome antigen for inducing short-lived antibody response and schistosomiasis diagnostic kit and detection method for detecting antibody response |
| WO2024012540A1 (en) * | 2022-07-13 | 2024-01-18 | 中国人民解放军海军军医大学 | Anti-tumor effect, preparation and use of schistosoma japonicum eggs and secreted and excreted proteins thereof |
-
2008
- 2008-12-11 CN CN200810044093A patent/CN101748140A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667200A (en) * | 2012-09-06 | 2014-03-26 | 江苏省血吸虫病防治研究所 | Schistosome antigen for inducing short-lived antibody response and schistosomiasis diagnostic kit and detection method for detecting antibody response |
| WO2024012540A1 (en) * | 2022-07-13 | 2024-01-18 | 中国人民解放军海军军医大学 | Anti-tumor effect, preparation and use of schistosoma japonicum eggs and secreted and excreted proteins thereof |
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Open date: 20100623 |