CN101748139A - Schistosoma japonica enolase gene and coding protein and application thereof - Google Patents
Schistosoma japonica enolase gene and coding protein and application thereof Download PDFInfo
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- CN101748139A CN101748139A CN200810044090A CN200810044090A CN101748139A CN 101748139 A CN101748139 A CN 101748139A CN 200810044090 A CN200810044090 A CN 200810044090A CN 200810044090 A CN200810044090 A CN 200810044090A CN 101748139 A CN101748139 A CN 101748139A
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- albumen
- seq
- enolase
- schistosoma
- japonica
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a schistosoma japonica enolase gene and a coding protein and an application thereof. The schistosoma japonica enolase gene comprises nucleotide sequences shown by SEQ ID No. 1-SEQ ID No. 3; and schistosoma japonica enolase comprises a protein with nucleotide sequences shown by SEQ ID No. 4-SEQ ID No. 6 or a protein with the same function formed by replacing and deleting or inserting the protein. In addition, the invention also discloses a schistosoma japonica enolase gene and an application of a coding protein thereof. The schistosoma japonica enolase can be applied to schistosome vaccine preparation, medicament screening, anti-schistosoma antibody preparation and serodiagnosis; and the schistosoma japonica superoxide dismutase gene can be applied to gene therapy.
Description
Technical field
The present invention relates to field of biological genes, relate to a kind of Schistosoma japonica enolase gene, its proteins encoded and application particularly.
Background technology
Schistosomicide is caused that by bilharzial infection 76 countries are arranged in the world, and the sufferer about 1.5 hundred million.Morbific schistosomicide has 3 kinds, both Schistosoma japonicum (Schistosoma japonicum), Schistosoma mansoni (S.mansoni) and Schistosoma haematobium (S.haematobium).At China's popular is Schistosoma japonicum.The popular of schistosomicide all causes tremendous loss for people's health and Economic development, and therefore, prevention and cure of schistosomiasis, development schistosomicide reagent for clinical diagnosis and antischistosomal medicine have huge social and economic benefit.
Can there be a lot of years in Schistosoma japonicum as parasite and not be killed by host's immunologic mechanism in human body and animal body, relevant with its immune evasion ability.Its immune evasion mechanism has antigen mimicking, antigen camouflage and immunomodulatory.Wherein immunomodulatory refers to the immunity system that (mainly being to suppress) host can be regulated, be disturbed to bilharzial albumen, makes its immune attack usefulness that can not bring into normal play, thereby helps bilharzial existence.
(Excretory/Secretory protein ESP) is exactly the protein that a class can be brought into play immunoregulation effect to bilharzial drainage/secretion antigen.These albumen are from bilharzial epidermis and gut epithelium, or the drainage/secretory of specialization.Each worm phase of schistosomicide all produces this proteinoid.ESP is potential drug target spot and vaccine molecule, thereby can disturb bilharzial immune evasion mechanism enhancing host's immunity to kill function because suppress these albumen; ESP is a potential diagnosis molecule, because these albumen are free in host's the blood, can detect with existing means easily.
Summary of the invention
One of the technical problem to be solved in the present invention provides a kind of Schistosoma japonica enolase gene.
Two of the technical problem to be solved in the present invention provides a kind of proteins encoded of Schistosoma japonica enolase gene.
Three of the technical problem to be solved in the present invention provides the application of above-mentioned kind of Schistosoma japonica enolase gene and proteins encoded.
In order to solve the problems of the technologies described above, the present invention is achieved through the following technical solutions:
In one aspect of the invention, provide a kind of Schistosoma japonica enolase gene, having comprised: had the nucleotide sequence shown in SEQ IDNO.1～SEQ ID NO.3.
Nucleotide sequence coded albumen shown in the described SEQ ID NO.1 with sequence shown in the SEQ ID NO.4; Nucleotide sequence coded albumen shown in the described SEQ ID NO.2 with sequence shown in the SEQ ID NO.5; Nucleotide sequence coded albumen shown in the described SEQ ID NO.3 with sequence shown in the SEQ ID NO.6.
In another aspect of this invention, provide a kind of Schistosoma japonica enolase, having comprised:
Have the albumen of aminoacid sequence shown in the SEQ ID NO.4 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.5 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.6 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen.
Schistosoma japonica enolase is atypical secretory protein, can be secreted in host's blood by schistosomicide, and performance immunoregulation effect, we are with online software SecretomeP 2.0 (http
The www.cbs.dtu.dk/ of: // Services/SecretomeP/) the prediction discovery, the NN-score value of Hydratase, phosphoenolpyruvate is 0.511, and the Hydratase, phosphoenolpyruvate that Schistosoma japonicum is described is atypical secretory protein, i.e. and this proteic secretion is not the signal peptide (classical mode) by albumen n end, but secretes cell by other approach.This explanation detects japonica enolase in host's serum be not accidental.
Traditional view thinks that Hydratase, phosphoenolpyruvate is intracellular glycolytic ferment, and the recent Hydratase, phosphoenolpyruvate that studies show that is multi-functional interior albumen.This enzyme is except being positioned in the cell, the surface that also is present in cell, for schistosomicide, this kind of enzyme or epidermal protein, protokaryon, Eukaryotic Hydratase, phosphoenolpyruvate can and Profibrinolysin (plasminogen) combination, bilharzial Hydratase, phosphoenolpyruvate also can be in conjunction with the Profibrinolysin in the blood.Schistosomicide excretory Hydratase, phosphoenolpyruvate may stimulate fibrinolytic activity, is beneficial to schistosomicide invasion and intravital mobile the host.
The present invention utilizes the proteomics means, and we have analyzed the moiety of ESP of the vitro extraction of Schistosoma japonicum in the world first.We find that the Hydratase, phosphoenolpyruvate (Enolase) of Schistosoma japonicum is one of main component of its ESP, show that this albumen may play a significant role aspect bilharzial immunomodulatory, the immune evasion; Simultaneously, by the blood of direct analysis infection animal (rabbit), find that this albumen also exists really in host's blood, this also makes it become good diagnostic flag molecule.
In another aspect of this invention, provide the purposes of Schistosoma japonica enolase, having comprised:, can be applicable to prepare blood fluke vaccine as immunogen; As the potential drug target, be applied to screening chemistry and other kind medicines; As the specificity schistosome antigen, be applied to prepare schistosomicide antibody; Schistosoma japonica enolase also can be applicable to the serodiagnosis aspect; Schistosoma japonica enolase gene can be applicable to the gene therapy aspect.
Embodiment
The present invention is further detailed explanation below in conjunction with drawings and Examples.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, people such as Sambrook for example, molecular cloning: laboratory manual (New York:ColdSpring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.
Embodiment proteic separation of 1 Schistosoma japonicum excretion and identification experiment
1). the preparation and the protein extraction of Schistosoma japonicum adult excretion product:
Schistosoma japonicum adult is separated the back in the infection animal body and is washed 2 times with PBS, and about 800 adults suspend with 1mlPBS, and low-speed centrifugal is collected the supernatant sucking-off after 10 minutes, repeats 10 and compiles, and supernatant is merged.With the albumen in the acetone precipitation supernatant, dissolution precipitation 50mM Tris-HCl (pH 8.3), 5mM EDTA, 1mM phenylmethylsulfonylfluoride (PMSF), 0.5%sodium dodecyl sulfate (SDS) and 0.5mM DTT, 0.5%SDS, ultrasonic short molten.
2). the proteic enzymolysis process of Schistosoma japonicum excretion:
The excretion egg white mixture spends the night with the pancreatin enzymolysis with the iodacetyl ammonia treatment of 10mM DTT and 55mM, dilution back.
3). the on-line chromatograph of Schistosoma japonicum excretion proteolysis peptide section separates and mass spectrum is identified:
The peptide mixt that enzymolysis produces pumps 30 μ l with vacuum with liquor capacity.Afterwards, on the peptide mixt of each component sample to anti-phase catch post (C18,5 μ m,
300 μ m i.d * 5mm, LC Packings), desalination under the flow velocity of 20 μ l/min.This 75 μ m * 150mm C18 post (LC Packings) of catching post and an analysis usefulness is connected then, just under the flow velocity of 200nl/min, wash-out enters the QSTAR pulser i of the electric spray ion source that Protana NanoES is housed peptide mixt from this pillar.Agilent 1100 capillary liquid phase systems (Agilent Technologies) are used to provide mobile phase A (0.5% acetic acid/water) and Mobile phase B (0.5% acetate/acetonitrile), linear gradient be in 60 minutes 5%B to 50%B, 50%B is to stop 15 minutes at 90%B to 90%B then in 30 minutes.The PicoTip that connects a 10-μ m diameter by one stainless two logical (Valco Instrument) behind analytical column receives fog-spray nozzle (New Objective), adds the spray voltage of 2500-3000 volt, to obtain stable spraying.MS/MS tandem mass spectrum spectrogram comes record by collection (IDA) and the task-circulation enhancing that information relies on.Measure in the scanning at each, choose 3-6 signal the strongest, with the ion of 2 to 3 electric charges, smash with the rolling collision energy, with the information of acquisition tandem mass spectrum.
4). the proteic software search of Schistosoma japonicum excretion is identified:
The database of mass spectrum search usefulness comprises the Schistosoma japonicum protein sequence of downloading from public database NCBI from 11717 (time is on October 16th, 2008).Search software is MASCOT (http://www.matrixscience.com/, Matrix Science).Search parameter is: maximum leakage point of contact, 3; Monoisotopic; The peptide charge number ,+1/+2/+3; Modify halfcystine carbamoylmethylation and methionine oxidation.After Search Results merges, carry out filtering screening: 1. to the search result list of each tandem mass spectrum spectrogram, only extract the result of ranking, other then abandon at first with following standard; 2. if in the peptide section that search obtains KR is arranged, KK, RK, sequences such as RR, then this peptide section abandons; 3. search for the peptide section that obtains, length is 8 reservations more than the amino acid, less than 8 amino acid whose abandoning; 4. repeat the peptide section got to, only calculate that wherein score value is the highest, these peptide section score values are added up, as proteic score; 5. proteic score surpasses 60, just thinks believable result.The low albumen that divides keeps after manual the inspection or abandons.Match host or bovine trypsin, keratic peptide section, then abandon.
Carry out the search of reverse protein sequence library simultaneously.Oppositely protein pool is with the counter-rotating of Schistosoma japonicum protein sequence, keep length, form constant.According to forward storehouse and the result that reverse library searching obtains, determine false positive rate.Method of calculation: false positive=2* (oppositely storehouse peptide hop count)/(forward storehouse peptide hop count+reverse storehouse peptide hop count).False positive is controlled at 0%.
5). the proteic proteomics qualification result of the external excretion of Schistosoma japonicum
Identify 110 schistosomicide albumen altogether.Wherein Hydratase, phosphoenolpyruvate is one of higher albumen of relative abundance, and the SC sum is 32, and peptide section score summation is 1750, identifies this proteic 8 kinds of peptide sections (table 1) altogether.This explanation, Hydratase, phosphoenolpyruvate is one of main protein ingredient in the Schistosoma japonicum adult excretion product.Adult is in host (as the people) body, and its excretion product must be discharged in host's the blood circulation system, therefore, detects the existence of schistosomicide excretion product, just can detect schistosomicide.And the high albumen of abundance in the excretion product, the easiest being detected, therefore, Hydratase, phosphoenolpyruvate is suitable clinical diagnosis molecule, can utilize existing universal method such as ELISA to detect schistosomicide characteristic protein in patient's blood sample easily.
The mass spectrum qualification result in external excretion protein groups of table 1. Schistosoma japonica enolase
The excretion albumen of embodiment 2 direct analysis Schistosoma japonicum from infection animal serum
1. schistosoma japonicum cercariae infects rabbit, collects and infects the 42nd day the animal serum in back.Form with the liquid chromatography-mass spectrography analysing protein behind the serum protein enzymolysis.The Protein Data Bank of mass-spectrometric data search Schistosoma japonicum is according to above-mentioned standard screening peptide section.The peptide section that obtains is removed the peptide section of any coupling with the genes database (comprising mRNA, EST, gene order-checking data etc.) of Blast search rabbit, and the peptide section that remains all is that schistosomicide is distinctive.Utilize the peptide section then, search obtains corresponding schistosomicide protein sequence.
2. albumen abundance quantitative analysis: (Spectra count SC), represents the abundance height with this to calculate the mass spectrum sum that each Identification of Fusion Protein obtains.
3. the qualification result of Schistosoma japonica enolase in infection animal serum
From infected animal serum, directly identify the excretion albumen of Schistosoma japonicum in the world first.Identify 2 kinds of peptide sections (table 2) of Hydratase, phosphoenolpyruvate, proved that the Hydratase, phosphoenolpyruvate of Schistosoma japonicum is present in the serum of host animal really, can be detected, thereby can become the target molecules of schistosomicide clinical diagnosis.
Table 2, the qualification result of Schistosoma japonica enolase in infection animal serum
Sequence table
＜110〉Research Center of Shanghai Human Genome
＜120〉Schistosoma japonica enolase gene, its proteins encoded and application
<130>NP-08-12787
<160>6
<170>PatentIn?version?3.3
<210>1
<211>2044
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>1
atgacatttt?actccacatc?ggttcagcat?aattcgtatg?aattcccgtt?gataggctaa 60
cgaattatat?tctcgatggc?tcatcttcga?gttggtgcag?atttcattgc?atcttacagg 120
tctcgtttta?atcagatggg?tgatctgcga?aaaaagctaa?aactgttttc?tttcaacact 180
agctattaca?atcaagtcgg?actactaaaa?cacgattggc?tccctcatag?tcctgcattg 240
gttgaagctc?ttcgtcgact?cccacgtgat?gaactggagg?ctagggattt?tcgaatcgct 300
cgagcttcac?tgctttatgc?ctcaaaaaat?attttgccaa?aggagcaatg?gacaacaata 360
gaaaatgata?ttccttattt?ggatccatat?attaatgttg?ttgtaaagga?acatcatgac 420
cagacaagtt?gggacaattt?tgtcagccct?gtaatatata?gaggaccata?aacatttgtt 480
ttcttttagt?acttagctaa?ataaaagttt?tattaaaggc?cttgttctca?ttatcttatt 540
ttttggtgta?gctaactatc?cttgtttctc?ttagaaaatg?gcaattatag?cgattcacgc 600
ccgtcaaatc?ttcgacagcc?gagggaatcc?cacagttgag?gttgatctaa?aaacagcaaa 660
aggtttgttc?cgcgcagccg?ttccaagtgg?ggcttcaaca?ggtgttcatg?aggctcttga 720
attacgtgat?acaaattcaa?aggcttatat?gtgcaaaggg?gttttgacag?cagttagtaa 780
tgtgaataat?atcatcgctc?cagctctact?gaagaaacaa?attcctgtaa?ccaatcaatc 840
ggaggttgac?caatttatga?tcgaactcga?tggcaaagaa?aacaaaggaa?atctcggagc 900
gaatgctata?ttaggcgtat?ctttggctgt?atgcaaggcg?ggtgcagctg?aattaaattt 960
acctctctat?aggtatattg?caaagttagc?tggccataag?gatgtcataa?tgccggttcc 1020
tgcatttaat?gtcattaatg?gtggcagtca?tgctggcaac?aagctagcga?tgcaggagtt 1080
tatgatttta?cctactgggg?ctagctcatt?cactgaggca?atgcaaatgg?gttctgaggt 1140
gtaccacaat?ttaaaggctg?ttattagacg?tgagtttgga?ttagatgcat?gtaacgttgg 1200
tgacgagggt?ggttttgcac?cgaatatcca?ggataatatg?aaaggtcttc?agcttcttga 1260
agaggctata?aaaattgctg?gatatactgg?aaaagtagag?attggtatgg?attgcgcggc 1320
ttctgaatat?tataagaagg?ggaagtatga?tttggatttc?aagaatcctc?aatctgccga 1380
atctcattgg?cttagtcctg?atgaaatggc?taatgtttac?aaggaaatga?ttcagaagta 1440
cccaatagtg?agcattgagg?acccgtttga?tcaagatgat?tgggatgcgt?ggcctaaatt 1500
gactgcatca?actaacattc?agattgtcgg?cgacgactta?acagtcacca?atccgaaacg 1560
cattgaaaag?gccatcaaag?taaaagcttg?caattgcctt?cttttgaaag?taaaccagat 1620
aggttcaata?accgagtcta?ttgaagcttg?caaaatggca?caaaaagcag?gctggggtgt 1680
tatggtttca?catcgatcag?gtgaaacgga?agataatttc?atcgctgatc?ttgtcgttgg 1740
tctttgtact?ggacagatca?aaacaggtgc?accttgtcga?tttgaacgtc?ttgcgaagta 1800
caaccaactc?ttgcgtattg?aagaggaatt?aggatcaaca?gcaaagtatg?ccggaaaaca 1860
cttccgccat?cctcaaattt?gaacaaataa?taatattaac?atttcaatgt?cttttgaagg 1920
actgcattga?tttagtttat?tgtcttatca?tgtagactgt?tatttaaaag?aaattaaatg 1980
tttgaattgt?tcccacattt?atattaatag?agcgtcgaag?ttatcagttt?ggtaaataaa 2040
aaaa 2044
<210>2
<211>1482
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>2
tcttgtgatt?tgttgcatgc?taactatcct?tgtttctctt?agaaaatggc?aattatagcg 60
attcacgccc?gtcaaatctt?cgacagccga?gggaatccca?cagttgaggt?tgatctaaaa 120
acagcaaaag?gtttgttccg?cgcagccgtt?ccaagtgggg?cttcaacagg?tgttcatgag 180
gctcttgaat?tacgtgatac?aaattcaaag?gcttatatgt?gcaaaggggt?tttgacagca 240
gttagtaatg?tgaataatat?catcgctcca?gctctgctga?agaaacaaat?tcctgtaacc 300
aatcaatcgg?aggttgacca?atttatgatc?gaactcgatg?gcaaagaaaa?caaaggaaat 360
ctcggagcga?atgctatatt?aggcgtatct?ttggctgtat?gcaaggcggg?tgcagctgaa 420
ttaaatttac?ctctctatag?gtatattgca?aagttagctg?gccataagga?tgtcataatg 480
ccggttcctg?catttaatgt?cattaatggt?ggcagtcatg?ctggcaacaa?gctagcgatg 540
caggagttta?tgattttacc?tactggggct?agctcattca?ctgaggcaat?gcaaatgggt 600
tctgaggtgt?accacaattt?aaaggctgtt?attaaacgtg?agtttggatt?agatgcatgt 660
aacgttggtg?acgagggtgg?ttttgcaccg?aatatccagg?ataatatgaa?aggtcttcag 720
cttcttgaag?aggctataaa?aattgctgga?tatactggaa?aagtagagat?tggtatggat 780
tgcgcggctt?ctgaatatta?taagaagggg?aagtatgatt?tggatttcaa?gaatcctcaa 840
tctgccgaat?ctcattggct?tagtcctgat?gaaatggcta?atgtttacaa?ggaaatgatt 900
cagaagtacc?caatagtgag?cattgaggac?ccgtttgatc?aagatgattg?ggatgcgtgg 960
cctaaattga?ctgcgtcaac?taacattcag?attgtcggcg?acgacttaac?agtcaccaat 1020
ccgaaacgca?ttgaaaaggc?catcaaagta?aaagcttgca?attgccttct?tttgaaagta 1080
aaccagatag?gttcaataac?cgagtctatt?gaagcttgca?aaatggcaca?aaaagcaggc 1140
tggggtgtta?tggtttcaca?tcgatcaggt?gaaacggaag?ataatttcat?cgctgatctt 1200
gtcgttggtc?tttgtactgg?acagatcaaa?acaggtgcac?cttgtcgatc?tgaacgtctt 1260
gcgaagtaca?accaactctt?gcgtattgaa?gaggaattag?gatcaacagc?aaagtatgcc 1320
ggaaaacact?tccgccatcc?tcaaatctga?acaaataata?atattaacat?ttcaatgtct 1380
tttgaaggac?tgcattgatt?tagtttattg?tcttatcatg?tagactgtta?tttaaaagaa 1440
attaaatgtc?tgaattgttc?ccaataaaaa?aaaaaaaaaa?aa 1482
<210>3
<211>1345
<212>RNA
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>3
tttactcttg?tgatttgttg?catgaaaatg?gcaattatag?cgattcacgc?ccgtcaaatc 60
ttcgacagcc?gagggaatcc?cacagttgag?gttgatctaa?aaacagcaaa?aggtttgttc 120
cgcgcagccg?ttccaagtgg?ggcttcaaca?ggtgttcatg?aggctcttga?attacgtgat 180
acaaattcaa?aggcttatat?gtgcaaaggg?gttttgacag?cagttagtaa?tgtgaataat 240
atcatcgctc?ctgctctgct?gaagaaacaa?attcctgtaa?ccaatcaatc?ggaggttgac 300
caatttatga?tcgaactcga?tggcaaagaa?aacaaaggaa?atctcggagc?gaatgctata 360
ttaggcgtat?ctttggctgt?atgcaaggcg?ggtgcagctg?aattaaattt?acctctctat 420
aggtatattg?caaagttagc?tggccataag?gatgtcataa?tgccggttcc?tgcatttaat 480
gtcattaatg?gtggcagtca?tgctggcaac?aagctagcga?tgcaggagtt?tatgatttta 540
cctactgggg?ctagctcatt?cactgaggca?atgcaaatgg?gttctgaggt?gtaccacaat 600
ttaaaggctg?ttatcaaacg?tgagtttgga?ttagatgcat?gtaacgttgg?tgacgagggt 660
ggttttgcac?cgaatatcca?ggataatatg?aaaggtcttc?aacttcttga?agaggctata 720
aaaattgctg?gatatactgg?aaaagtagag?attggtatgg?attgcgcggc?ttctgaatat 780
tataagaagg?ggaagtatga?tttggatttc?aagaatcctc?aatctgccga?atctcattgg 840
cttagtcctg?atgaaatggc?taatgtttac?aaggaaatga?ttcagaagta?cccaatagtg 900
agcattgagg?acccgtttga?tcaagatgat?tgggatgcgt?ggcctaaatt?gactgcgtca 960
actaacattc?agattgtcgg?cgacgactta?acagtcacca?atccgaaacg?cattgaaaag 1020
gccatcaaag?taaaagcttg?caattgcctt?cttttgaaag?taaaccagat?aggttcaata 1080
accgagtcta?ttgaagcttg?caaaatggca?caaaaagcag?gctggggtgt?tatggtttca 1140
catcgatcag?gtgaaacgga?agataatttc?atcgctgatc?ttgtcgttgg?tctttgtact 1200
ggacagatca?aaacaggtgc?accttgtcga?tttgaacgtc?ttgcgaagta?caaccaactc 1260
ttgcgtattg?aagaggaatt?aggatcaaca?gcaaagtatg?ccggaaaaca?cttccgccat 1320
cctcaaattt?gaacaaataa?taata 1345
<210>4
<211>434
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>4
MAIIAIHARQ?IFDSRGNPTV?EVDLKTAKGL?FRAAVPSGAS?TGVHEALELR?DTNSKAYMCK 60
GVLTAVSNVN?NIIAPALLKK?QIPVTNQSEV?DQFMIELDGK?ENKGNLGANA?ILGVSLAVCK 120
AGAAELNLPL?YRYIAKLAGH?KDVIMPVPAF?NVINGGSHAG?NKLAMQEFMI?LPTGASSFTE 180
AMQMGSEVYH?NLKAVIRREF?GLDACNVGDE?GGFAPNIQDN?MKGLQLLEEA?IKIAGYTGKV 240
EIGMDCAASE?YYKKGKYDLD?FKNPQSAESH?WLSPDEMANV?YKEMIQKYPI?VSIEDPFDQD 300
DWDAWPKLTA?STNIQIVGDD?LTVTNPKRIE?KAIKVKACNC?LLLKVNQIGS?ITESIEACKM 360
AQKAGWGVMV?SHRSGETEDN?FIADLVVGLC?TGQIKTGAPC?RFERLAKYNQ?LLRIEEELGS 420
TAKYAGKHFR?HPQI 434
<210>5
<211>434
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>5
MAIIAIHARQ?IFDSRGNPTV?EVDLKTAKGL?FRAAVPSGAS?TGVHEALELR?DTNSKAYMCK 60
GVLTAVSNVN?NIIAPALLKK?QIPVTNQSEV?DQFMIELDGK?ENKGNLGANA?ILGVSLAVCK 120
AGAAELNLPL?YRYIAKLAGH?KDVIMPVPAF?NVINGGSHAG?NKLAMQEFMI?LPTGASSFTE 180
AMQMGSEVYH?NLKAVIKREF?GLDACNVGDE?GGFAPNIQDN?MKGLQLLEEA?IKIAGYTGKV 240
EIGMDCAASE?YYKKGKYDLD?FKNPQSAESH?WLSPDEMANV?YKEMIQKYPI?VSIEDPFDQD 300
DWDAWPKLTA?STNIQIVGDD?LTVTNPKRIE?KAIKVKACNC?LLLKVNQIGS?ITESIEACKM 360
AQKAGWGVMV?SHRSGETEDN?FIADLVVGLC?TGQIKTGAPC?RSERLAKYNQ?LLRIEEELGS 420
TAKYAGKHFR?HPQI 434
<210>6
<211>436
<212>PRT
＜213〉Schistosoma japonicum (Schistosoma japonicum)
<400>6
MKMAIIAIHA?RQIFDSRGNP?TVEVDLKTAK?GLFRAAVPSG?ASTGVHEALE?LRDTNSKAYM 60
CKGVLTAVSN?VNNIIAPALL?KKQIPVTNQS?EVDQFMIELD?GKENKGNLGA?NAILGVSLAV 120
CKAGAAELNL?PLYRYIAKLA?GHKDVIMPVP?AFNVINGGSH?AGNKLAMQEF?MILPTGASSF 180
TEAMQMGSEV?YHNLKAVIKR?EFGLDACNVG?DEGGFAPNIQ?DNMKGLQLLE?EAIKIAGYTG 240
KVEIGMDCAA?SEYYKKGKYD?LDFKNPQSAE?SHWLSPDEMA?NVYKEMIQKY?PIVSIEDPFD 300
QDDWDAWPKL?TASTNIQIVG?DDLTVTNPKR?IEKAIKVKAC?NCLLLKVNQI?GSITESIEAC 360
KMAQKAGWGV?MVSHRSGETE?DNFIADLVVG?LCTGQIKTGA?PCRFERLAKY?NQLLRIEEEL 420
GSTAKYAGKH?FRHPQI 436
Claims (8)
1. a Schistosoma japonica enolase gene is characterized in that: comprising: have the nucleotide sequence shown in SEQ ID NO.1～SEQ IDNO.3.
2. Schistosoma japonica enolase gene as claimed in claim 1 is characterized in that: the albumen of sequence shown in the nucleotide sequence coded SEQ of the having ID NO.4 shown in the described SEQ IDNO.1; Nucleotide sequence coded albumen shown in the described SEQ ID NO.2 with sequence shown in the SEQ ID NO.5; Nucleotide sequence coded albumen shown in the described SEQ ID NO.3 with sequence shown in the SEQ ID NO.6.
3. Schistosoma japonica enolase is characterized in that: comprising:
Have the albumen of aminoacid sequence shown in the SEQ ID NO.4 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.5 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen;
Have the albumen of aminoacid sequence shown in the SEQ ID NO.6 or one or more amino acid whose displacements, disappearance or insertion take place and the albumen that forms with identical function by this albumen.
4. the application of Schistosoma japonica enolase in the preparation blood fluke vaccine.
5. the application of Schistosoma japonica enolase in screening of medicaments.
6. the application of Schistosoma japonica enolase in preparation schistosomicide antibody.
7. the application of Schistosoma japonica enolase aspect serodiagnosis.
8. the application of Schistosoma japonica enolase gene aspect gene therapy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810044090A CN101748139A (en) | 2008-12-11 | 2008-12-11 | Schistosoma japonica enolase gene and coding protein and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810044090A CN101748139A (en) | 2008-12-11 | 2008-12-11 | Schistosoma japonica enolase gene and coding protein and application thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN101748139A true CN101748139A (en) | 2010-06-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200810044090A Pending CN101748139A (en) | 2008-12-11 | 2008-12-11 | Schistosoma japonica enolase gene and coding protein and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611296A (en) * | 2015-01-22 | 2015-05-13 | 江苏省血吸虫病防治研究所 | Hybridoma for secreting anti-recombinant schistosoma japonica enolase specific monoclonal antibody as well as preparation method and application of hybridoma |
| CN106906229A (en) * | 2017-04-27 | 2017-06-30 | 江汉大学 | The enolase gene OsENO2 2 and its application of influence rice anthesis and grain type |
-
2008
- 2008-12-11 CN CN200810044090A patent/CN101748139A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104611296A (en) * | 2015-01-22 | 2015-05-13 | 江苏省血吸虫病防治研究所 | Hybridoma for secreting anti-recombinant schistosoma japonica enolase specific monoclonal antibody as well as preparation method and application of hybridoma |
| CN106906229A (en) * | 2017-04-27 | 2017-06-30 | 江汉大学 | The enolase gene OsENO2 2 and its application of influence rice anthesis and grain type |
| CN106906229B (en) * | 2017-04-27 | 2020-04-10 | 江汉大学 | Enolase gene OsENO2-2 influencing flowering period and grain type of rice and application thereof |
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Open date: 20100623 |