CN101743918A - Subcutaneous alizarin red S marking method for sepiella maindroni and special marking reagent thereof - Google Patents
Subcutaneous alizarin red S marking method for sepiella maindroni and special marking reagent thereof Download PDFInfo
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- CN101743918A CN101743918A CN200910157167A CN200910157167A CN101743918A CN 101743918 A CN101743918 A CN 101743918A CN 200910157167 A CN200910157167 A CN 200910157167A CN 200910157167 A CN200910157167 A CN 200910157167A CN 101743918 A CN101743918 A CN 101743918A
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- sepiella maindroni
- alizarin red
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a subcutaneous alizarin red S marking method for sepiella maindroni. The method comprises the following steps: firstly, anesthesia step: selecting sepiella maindroni to be marked into MS-222 anesthetic solution with a concentration of 40ppm to 70ppm for 0.5 to 4 minutes; secondly, marking step: injecting dyeing liquor into the inner shell of the sepiella maindroni treated in step one with an injector. Because the MS-222 anesthetic solution is used for performing anesthesia processing to the sepiella maindroni, the sepiella maindroni basically has no stress when being injected with dyeing liquor, thereby being convenient for operation and reducing the hurt to the sepiella maindroni. The special marking reagent for the subcutaneous alizarin red S marking method for sepiella maindroni comprises 1 part of alizarin red S powder and 4-8 parts of yoghourt according to part by weight, marks can be formed in relatively concentrated subcutaneous areas and are more highly identifiable than the ordinary dyeing marks. In addition, by using the method, after being resuscitated, the sepiella maindroni is not required to be raised temporarily to remove the floating color but can be freed immediately.
Description
Technical field
The present invention relates to labeling method and the special marker reagent thereof of a kind of aquatic animal, particularly subcutaneous alizarin red S labeling method of Sepiella maindroni and special marker reagent thereof.
Background technology
Sepiella maindroni once was one of the four big fishery in China fishing ground, and was stronger to the adaptability of temperature, in China coastal distribution very wide, be the very high traditional fishery kind of a kind of economic worth.Because overfishing and ecological degeneration since the seventies in 20th century, decay successively occurred.Last century, the eighties had the Primary Study to Sepiella maindroni resource and propagation such as report Li Xing a word used in person's names, and abroad had the biology of merchant cuttlefish and the report of breed aspect.So far, about scientific research institution has tentatively solved Sepiella maindroni genital regulating, artificial breeding, seed rearing and has formed technology, for the maintenance of Sepiella maindroni resource provides important basis.In order behind artificial breeding, natural population to be carried out maintenance, need a kind of effect stability, labeling method simple to operate, be beneficial in theory the effect of releasing be assessed, and then optimize the propagation scheme of releasing.The characteristics of the cephalopodous work of releasing are at present only paid attention to releasing to the sea area, and ignore the effect of releasing, and Study on Technology such as tracking spike but relatively lag behind.Also there is not open report at present both at home and abroad about the Sepiella maindroni labeling technique.Prior art such as Chinese invention patent " a kind of method that indicates golden cuttlefish ", patent No. ZL200610070448.6, a kind of method that indicates golden cuttlefish is disclosed, it is characterized in that the golden cuttlefish after the hatching was supported 5-20 days temporarily, coloring agent is dissolved in fresh water, put into the aqueous solution of the coloring agent that makes in the container that fills cuttlefish and seawater, the concentration that makes coloring agent in the above-mentioned seawater is 60-80ppm, soaking and dyeing 20-28 hour.Described coloring agent is the alizarin complexometric indicator.Method of the present invention is easy and simple to handle, and discrimination method is simple, nontoxic not damaged, and can disposablely dye in a large number and handle and be difficult for fading, be a kind of good method that indicates golden cuttlefish.Yet; its mark DeGrain; and the mark loose colour can short-term residue in body surface; to discharge to the nature marine site through the cuttlefish of this method mark and influence the protective coloration of itself; be unfavorable for the survival in its natural environment; and this method is carried out dye marker by the pin portion of contaminating golden cuttlefish to its inner casing tail end, and the individuality that this method belongs to for sepiella maindroni de Rochebrune will be no longer suitable.
Summary of the invention
The purpose of this invention is to provide the subcutaneous alizarin red S labeling method of Sepiella maindroni; solve the technical problem of Sepiella maindroni releasing mark with this; and this method is simple and practical, safely and fast; and stress not; the color of label does not residue in body surface, and is less relatively to the protective coloration influence of Sepiella maindroni own, helps release survival under the environment of nature; need not to support temporarily, can directly release.The present invention also provides the subcutaneous alizarin red S labeling method of Sepiella maindroni special marker reagent, and it has the cuttlefish stimulation little, infects and rejects and lack the permanent advantage of mark.
The subcutaneous alizarin red S labeling method of Sepiella maindroni of the present invention, it comprises the steps:
1. anaesthetize step: choose Sepiella maindroni to be marked and put into the MS-222 anesthetic solution 0.5 minute to 4 minutes that concentration is 40ppm to 70ppm;
2. markers step: inject the outer embrane cortex of the Sepiella maindroni of 1. handling through step with the syringe cream that will dye;
Dyeing cream contains alizarin red S.The step cream that 2. dyes also contains sour milk, and the weight ratio of alizarin red S and sour milk is 1: 4 to 1: 8; The amount of dyeing cream injection is less than 0.5ml; The syringe needle of syringe and inner shell of Sepiella maindroni surface are acute angle and insert; Step 2. in, syringe before use in 90% to 95% alcohol the sterilization 10 minutes to 15 minutes.
The present invention also provides the subcutaneous alizarin red S labeling method of Sepiella maindroni special marker reagent, wherein: contain 1 part alizarin red S powder and 4 parts to 8 parts sour milk according to parts by weight.Alizarin red S powder and sour milk adopt lapping mode to mix.
Compared with prior art, the invention has the advantages that: the present invention has used the MS-222 anesthetic solution that Sepiella maindroni has been carried out anaesthetic treatment, make its when accepting injection, do not have substantially stress, promptly be convenient to operate and reduced injury it.Evidence contains 1 part alizarin red S powder and the subcutaneous alizarin red S labeling method of the Sepiella maindroni of 4 parts to 8 parts sour milk special marker reagent according to parts by weight can form mark in the relative subcutaneous area of concentrating, and more general dye marker has higher identifiability.In addition, method of the present invention need not to support temporarily to remove removal floating color after the cuttlefish recovery, can release immediately.
Embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment 1: at first the 6ml syringe is placed the alcohol of the 90-95% 10-15min that sterilizes.Take out and draw 3.0ml pasty state ARS label afterwards.With trunk long for the cuttlefish individuality of 1.1cm put into the about 0.5min of MS-SSS anesthetic solution that concentration is 60ppm after taking-up immediately place on the operating desk that is covered with the towel that soaks.With syringe needle from lower end, the individual back of cuttlefish to cephalad direction to pierce through exocuticle sideling with the about 12 ° of angles of horizontal plane, syringe needle enters the about 0.3cm of exocuticle, makes it to mediate between film and the exocuticle.In the process of outwards pulling out pin suitable simultaneously try hard to recommend moving syringe with syringe in pasty state ARS label be injected into into strips under the cuttlefish exocuticle.After extracting syringe needle, immediately tagging is put into clean sea water and recover.
Embodiment 2: at first the 6ml syringe is placed the alcohol of the 90-95% 10-15min that sterilizes.Take out and draw 3.0ml pasty state ARS label afterwards.With trunk long for the cuttlefish individuality of 3.5cm put into the about 2.0min of MS-SSS anesthetic solution that concentration is 60ppm after taking-up immediately place on the operating desk that is covered with the towel that soaks.With syringe needle from the individual back of cuttlefish front end to caudal directions to pierce through exocuticle sideling with the about 12 ° of angles of horizontal plane, syringe needle enters the about 0.8cm of exocuticle, makes it to mediate between film and the exocuticle.In the process of outwards pulling out pin suitable simultaneously try hard to recommend moving syringe with syringe in pasty state ARS label be injected into into strips under the cuttlefish exocuticle.After extracting syringe needle, immediately tagging is put into clean sea water and recover.
Embodiment 3: at first the 10ml syringe is placed the alcohol of the 90-95% 10-15min that sterilizes.Take out and draw 3.0ml pasty state ARS label afterwards.With trunk long for the cuttlefish individuality of 6.2cm put into the about 3.5min of MS-SSS anesthetic solution that concentration is 60ppm after taking-up immediately place on the operating desk that is covered with the towel that soaks.With syringe needle from the individual back of cuttlefish front end to caudal directions to pierce through exocuticle sideling with the about 12 ° of angles of horizontal plane, syringe needle enters the about 1.2cm of exocuticle, makes it to mediate between film and the exocuticle.In the process of outwards pulling out pin suitable simultaneously try hard to recommend moving syringe with syringe in pasty state ARS label be injected into into strips under the cuttlefish exocuticle.After extracting syringe needle, immediately tagging is put into clean sea water and recover.
Experimental result shows that 175 trunks are long for the cuttlefish individuality of 2-3cm utilizes subcutaneous ARS labeling method mark, and the mark conservation rate is 100% behind the 5d, and with the naked eye can observe directly the phosphor strip mark at its back.Culture after two months to adult and begin to lay eggs.During this time every 10 days left and right sides sampling and measurings be labeled individual biological indicator such as total length, trunk is long, trunk is wide, body weight, shell is long, shell is wide, shell heavily waits, and compare with blank group cuttlefish individuality, the result shows both and there was no significant difference, and this labeling method growing and breed did not influence cuttlefish is described.Get rid of natural death and the individual influence of sampling in the breeding process, under this labeling method the survival rate of cuttlefish 95% or more and the mark conservation rate be 100%, and after originally being marked at injection,, be easy to discern at the permanent furuncle knot of the subcutaneous formation of cuttlefish.
Advantage of the present invention: the domestic and international at present report about the siphonopods labeling technique only has Hao Zhenlin (2007) to utilize fluorescent dye one alizarin complexometric indicator (ALC) the golden cuttlefish inner casing to be carried out the research work of mark, by golden cuttlefish is contaminated its pin portion and its inner casing tail end is carried out dye marker in ACL solution, the individuality that this method belongs to for sepiella maindroni de Rochebrune will be no longer suitable.Also not about the open report of sepiella maindroni de Rochebrune labeling technique, this labeling method not only can remedy domestic and international blank about the sepiella maindroni de Rochebrune labeling technique, and can solve all cephalopodous mark difficult problems both at home and abroad.This labeling method is simple to operation quick, and lethality is low, and mark look conservation rate is 100%, and mark identification is very convenient, need not to dissect, and can clearly see the strip fluorescence labeling at the individual back of cuttlefish by naked eyes.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.
Claims (7)
1. the subcutaneous alizarin red S labeling method of Sepiella maindroni is characterized in that: comprise the steps:
1. anaesthetize step: choose Sepiella maindroni to be marked and put into the MS-222 anesthetic solution 0.5 minute to 4 minutes that concentration is 40ppm to 70ppm;
2. markers step: inject the outer embrane cortex of the Sepiella maindroni of 1. handling through step with the syringe cream that will dye;
Described dyeing cream contains alizarin red S.
2. the subcutaneous alizarin red S labeling method of Sepiella maindroni as claimed in claim 1 is characterized in that: the 2. described dyeing cream of step also contains sour milk, and the weight ratio of described alizarin red S and described sour milk is 1: 4 to 1: 8.
3. the subcutaneous alizarin red S labeling method of Sepiella maindroni as claimed in claim 2 is characterized in that: step 2. in, the amount of dyeing cream injection is less than 0.5ml.
4. the subcutaneous alizarin red S labeling method of Sepiella maindroni as claimed in claim 3 is characterized in that: step 2. in, the syringe needle of syringe and inner shell of Sepiella maindroni surface are acute angle and insert.
5. the subcutaneous alizarin red S labeling method of Sepiella maindroni as claimed in claim 4 is characterized in that: step 2. in, syringe before use in 90% to 95% alcohol the sterilization 10 minutes to 15 minutes.
6. the subcutaneous alizarin red S labeling method of Sepiella maindroni special marker reagent is characterized in that: contain 1 part alizarin red S powder and 4 parts to 8 parts sour milk according to parts by weight.
7. the subcutaneous alizarin red S labeling method of Sepiella maindroni as claimed in claim 6 special marker reagent is characterized in that: described alizarin red S powder and sour milk adopt lapping mode to mix.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101571678A CN101743918B (en) | 2009-12-20 | 2009-12-20 | Special marking reagent for subcutaneous alizarin red S marking method for sepiella maindroni |
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| CN2009101571678A CN101743918B (en) | 2009-12-20 | 2009-12-20 | Special marking reagent for subcutaneous alizarin red S marking method for sepiella maindroni |
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| CN101743918A true CN101743918A (en) | 2010-06-23 |
| CN101743918B CN101743918B (en) | 2012-06-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372272A (en) * | 2017-07-21 | 2017-11-24 | 浙江海洋大学 | A kind of cuttlefish long-acting physical device for marking based on endoskeleton cuttlebone |
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2009
- 2009-12-20 CN CN2009101571678A patent/CN101743918B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372272A (en) * | 2017-07-21 | 2017-11-24 | 浙江海洋大学 | A kind of cuttlefish long-acting physical device for marking based on endoskeleton cuttlebone |
| CN107372272B (en) * | 2017-07-21 | 2020-10-13 | 浙江海洋大学 | Long-acting physical sign device of cuttlefish based on endoskeleton cuttlebone |
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| CN101743918B (en) | 2012-06-13 |
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