CN101731480A - Method for preparing vegetable feed additive for improving immunity of layer chicken - Google Patents
Method for preparing vegetable feed additive for improving immunity of layer chicken Download PDFInfo
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Abstract
The invention provides a method for preparing a vegetable feed additive for improving the immunity of layer chicken. The vegetable feed additive is a natural Chinese medicinal herb, namely glossy privet fruit, and the addition method thereof comprises the following steps of: baking the glossy privet fruit at the temperature of 65 DEG C to reach constant weight, crushing the baked glossy privet fruit for 40-mesh sieving, and then adding the sieved glossy privet fruit into daily ration of the layer chicken according to two dosages of 0.5 percent and 1 percent respectively. The glossy privet fruit has a positive promotion effect on the growth and development of the layer chicken during the incubation period by measuring the production performance, antioxidation function, specific immunity function (humoral immunity and cellular immunity) and biochemical indicators of egg quality and blood of the glossy privet fruit on the layer chicken (chicken, adult chicken and laying chicken). In the preparation method, the baked and crushed glossy privet fruit powder with two dosages of 0.5 percent and 1 percent is added into the daily ration of the layer chicken, so that the production performance, the antioxidation function, the immunity and the egg quality can be improved to a certain degree. The vegetable feed additive has the characteristics of low cost, simple production and use, little addition, obvious addition effect, no adverse reaction and the like.
Description
(1) technical field
The present invention relates to animal science, is exactly a kind of method for preparing vegetable feed additive that improves immunity of layer chicken specifically.
(2) background technology
Chinese herbal medicine uses as feed addictive, in the long history in existing several thousand of China, the record of " the paulownia leaf is raised pig, three times of hypertrophys, and easy supporting " is just arranged as China's medicine book " legendary god of farming vegetation warp " the earliest.Chinese herbal feed additive is according to China's traditional Chinese medicine theory, the scientific composition compatibility, not only have strengthening vital QI to eliminate pathogenic factors, spleen benefiting and stimulating the appetite, antibiotic growth promotion, strengthen the animal immune function, improve effect such as animal product quality, and wide material sources, have no side effect, noresidue, the no resistance to the action of a drug, caused the extensive interest of Chinese scholars, this precious legacy of motherland thereby be able to continuous excavation.Propose in " Chinese feed industry 1996～the year two thousand twenty development strategy ": " should keep punching and excavate the legacy of China Chinese herbal medicine aspect; particularly under the high situation of the world today ' recurrence ' natural voice day, wide prospect be arranged " with Chinese herbal medicine instead of chemical synthetic, antibiotics additive.Therefore, start the Study on Modernization and the industrialization development of natural Chinese medicinal herb additive comprehensively, substitute traditional Antibiotic Additive, for improving China's herding level of production, increasing the animal products export share, promote development and national economy, have great practice significance undoubtedly, not only imperative, and also extremely urgent.
(3) summary of the invention
The object of the present invention is to provide a kind of method for preparing vegetable feed additive that improves immunity of layer chicken.
The object of the present invention is achieved like this: described plant feed addictive is natural Chinese medicinal herb-fruit of glossy privet, its adding method is: the fruit of glossy privet is dried to constant weight through 65 ℃, pulverize, cross 40 mesh sieves, and add to respectively in the egg chick daily ration by 0.5%, 1% two dosage.
A kind of method for preparing vegetable feed additive that improves immunity of layer chicken of the present invention dries to constant weight the fruit of glossy privet through 65 ℃, pulverizes, and crosses 40 mesh sieves, and adds to respectively in the egg chick daily ration by 0.5%, 1% two dosage.Feeding experiment through certain phase, find that the fruit of glossy privet has by measuring the fruit of glossy privet after to laying hen (chick, become chicken and laying hen) production performance, anti-oxidation function, specific immune function (humoral immunity and cellular immunity), egg product matter and blood parameters: 1) growth of finishing period laying hen being grown has positive facilitation, better with the effect of adding 1% fruit of glossy privet; 2) fruit of glossy privet has the effect that strengthens finishing period laying hen immunologic function, add 1% fruit of glossy privet and can promote finishing period laying hen growth intermediary and later stages spleen and thymus development, can improve the effect of laying hen humoral immunity and cellular immunity, 1% fruit of glossy privet can improve laying hen serum newcastle epidemic disease antibody titre, prolong the duration, improve immunoglobulin (Ig) concentration, serum il-2 and TNF content in the serum, add the concentration that 0.5% fruit of glossy privet can improve serum complement C3 in preceding, mid-term, C4; 3) fruit of glossy privet can strengthen SOD, T-AOC, CAT, the GSH-PX activity of heart, liver and kidney, and can reduce MDA content in the tissue, reduces the infringement of MDA to body; 4) 0.5% fruit of glossy privet can improve protein content in the egg content, and reduces egg inner cholesterol content, and the mechanical index of egg product is had effect.The present invention mainly is the fruit of glossy privet powder after pulverizing by the drying of adding 0.5%, 1% two dosage in the chick daily ration, can improve production performance, anti-oxidation function, immunity and egg product matter to a certain extent.Product of the present invention have price low, produce and use characteristics such as simple, that addition is few, additive effect is obvious, have no adverse reaction.
(4) specific embodiment
Below the invention will be further described.
Embodiment 1: a kind of method for preparing vegetable feed additive that improves immunity of layer chicken of the present invention, described plant feed addictive is natural Chinese medicinal herb-fruit of glossy privet, its adding method is: the fruit of glossy privet is dried to constant weight through 65 ℃, pulverize, cross 40 mesh sieves, and add to respectively in the egg chick daily ration by 0.5% or 1% two dosage.Experiment content is as follows:
1. experimental animal and experimental design
Experimental animal is selected 1 age in days sea, 384 of blue brown egg chick (available from the long-range hatching in Harbin factory) for use, is hen, is divided into 4 groups at random, every group of 6 repetitions, and each repeats 16 chickens.Control group (I group) basal diet of feeding, test II～IV group is added the flavomycoin, 0.5% of 5mg/kg, 1% the fruit of glossy privet respectively in basic diet.Experimental basis diet nutrient content all meets or exceeds NRC (1994) standard, and diet is formed and trophic level sees Table 2-1 (1-18 week) and 2-2 (19-28 week).Experimental animal is raised in cages, free choice feeding and drinking-water, temperature, humidity and illumination in the control house.Finish since 1 age formal test to 28 week age in week, by a definite date 28 weeks.Experimental design sees Table 2-3.
The composition and the trophic level of table 2-1 basis diet
(1)Contain VA:7600IU in every kg diet, VD:100IU, VE:15mg, VK:2.6mg, VB
1: 1mg, VB
2: 8mg, VB
12: 0.05mg, folic acid: 0.8mg, nicotinic acid: 30mg, pantothenic acid: 7mg.
(2)Contain Cu:5mg in every kg diet, Mn:50mg, Fe:40mg, Zn:40mg, Se:0.15mg, I:0.15mg.
The experimental basis diet is corn one a dregs of beans type, needs preparation, Screening Experiment of Diet and trophic level (seeing Table 2-1) by Chinese poultry nutrition
Table 2-2 basis diet is formed and trophic level
1Contain VA:7600IU in every kg diet, VD:100IU, VE:15mg, VK:2.6mg, VB
1: 1mg, VB
2: 8mg, VB
12: 0.05mg, folic acid: 0.8mg, nicotinic acid: 30mg, pantothenic acid: 7mg.
2Contain Cu:5mg in every kg diet, Mn:50mg, Fe:40mg, Zn:40mg, Se:0.15mg, I:0.15mg.
Table 2-3 experimental design scheme
2. sample is collected
Each repeats to select 2 healthy chickens near average weight, venous blood collection under the wing, separation of serum in the 6th, 12,18 weeks of test, be put in the EP pipe,-20 ℃ of preservations are cutd open and are tried chicken extremely and win immune organ spleen, the bursa of farbricius, and thymus gland is also weighed, measure the immune organ index, adopt heart, liver and kidney simultaneously, grind, be diluted to 10% or 1% tissue homogenate with physiological saline with glass homogenizer,-20 ℃ of preservations, stand-by; In 18 weeks, each repeats to select 2 healthy chickens near average weight, and venous blood collection under the wing is used for the periphery lymphocyte conversion ratio and measures.
In 19 weeks of test injection bird flu H
5N
1Subgroup vaccine is in i.e. venous blood collection under 14,28, the 42 days wings in immunity back of 21,24,26 weeks, before the early morning feeding, each repeats to get at random 1, label, centrifugal 10 minutes of 3000r/min, three parts of preparation serum packing are in the eppendorf pipe, and-20 ℃ of refrigerators are preserved and are equipped with inspection.Same time when 22,24,26,28 weeks finished is gathered the egg sample respectively, and each repeats to randomly draw 4 pieces, and label is with getting with picking up.
3. detect index and method
(1) growth performance
In the 1st, 6,12,18 weeks of test each group chicken is weighed, 8h on an empty stomach before claiming, only for drinking-water, be that unit on an empty stomach weigh to repeat 8 of mornings next day, the clout of weighing simultaneously amount.And calculate each and organize daily gain, feed intake and the feed conversion rate that tries chicken.Formula is as follows:
Daily gain (g/d)=(end weight-starting weight)/test fate
Feed conversion rate=feed intake/daily gain
Begin to write down exactly every repetition egg number, egg production, broken soft egg number 19-28 week age, ramming material when finishing weekly calculates every repetition feed consumption rate, according to the feeding experiment record, and arrangement statistical average laying rate, average daily ingestion amount, feedstuff-egg ratio, broken soft egg rate.
Average laying rate (%)=total egg number/(chicken number * fate)
Average day egg production (a gram/day)=total egg size/(chicken number * fate)
Average daily ingestion amount (g/d)=total feed intake/(chicken number * fate)
Feedstuff-egg ratio=total feed intake/total egg size
Broken soft egg rate (%)=broken soft egg number/total egg number * 100%
Each issue on-test with when finishing, whenever repeat to randomly draw 2 chickens and take by weighing body weight, calculate changes of weight.
Changes of weight=end weight-first starting weight
(2) immunologic function index
During age in 1-18 week, measure that immune organ index, ewcastle disease hemagglutination inhibition antibody are tired, PBLC conversion ratio, serum immune globulin content, complement C3, C4 content, interleukin 2 content and TNF content.19-28 measures immunoglobulin (Ig), bird flu H in the serum during age in week
5N
1The hypotype blood clotting suppresses antibody titer.
4. result and analysis
Each growth performance index of handling egg chick different times sees Table 2-4.6 weeks during ages between 1% fruit of glossy privet group and control group, antibiotic group daily gain do not have significant difference (P＞0.05), but the daily gain of 0.5% fruit of glossy privet group significantly is lower than 1% fruit of glossy privet group (P＜0.05), does not have significant difference with control group, antibiotic group.During 12 ages in week, the daily gain of 0.5% fruit of glossy privet group has increase than control group and antibiotic group, but does not have significant difference (P＞0.05).During 18 ages in week, test group increases to some extent than the daily gain of control group, antibiotic group, and difference is remarkable (P＞0.05) not.In addition, 1 ~ 18 all daily gains between each processed group and feed conversion rate all do not have significant difference (P＞0.05).
The table 2-4 fruit of glossy privet is to the influence of egg chick production performance (gram/only)
Notes: colleague's shoulder mark different letter representation significant differences (P＜0.05), different capitalization differences are (P＜0.01) extremely significantly, and following table is together.
By table 2-5 as can be known, end body weight and changes of weight between interim each processed group of whole test all are increase trend, but biometrics difference is remarkable (P＞0.05) not, show that the fruit of glossy privet is not remarkable to the increase heavy influence of laying hen, but weightening finish trend maximum is 0.5% fruit of glossy privet group.
The table 2-5 fruit of glossy privet is to the influence of laying hen body weight
By table 2-6 as can be known, with the growth in age in laying hen week, all in various degree the risings of the laying rate of each processed group.Compare with the blank group, wherein 24 when week the I group laying rate improve 2.22%, each processed group influences difference not significantly (P＞0.05) to laying rate; Compare with the blank group, in 26 whens week, that I, II component be you can well imagine is high by 10.60%, 5.70%, and the I group is compared significant difference (P＜0.05) with the blank group, with II, III group difference not significantly (P＞0.05); During week, each processed group laying rate all reaches more than 90% at egg-laying peak 28, and all is higher than blank group, compares with blank, and the I group exceeds 7.50%, shows as significant difference (P＜0.05), and other respectively organize difference all not significantly (P＞0.05).Testing full phase laying rate does not have obvious influence (P＜0.05), and wherein the I group is higher than blank group 3.72%.
The table 2-6 fruit of glossy privet is to the influence of laying rate of laying hen and soft egg rate
2-6 knows by table, and from the full phase of whole test, the soft egg rate difference between each group is significantly (P＞0.05) not, and the broken soft egg rate of II group shows as minimum, and I group and III group all are lower than blank group at whole test phase indifference almost.The whole test phase, what broken soft egg rate was minimum is 1% fruit of glossy privet group.For the day egg production of laying hen, this test is represented with a day egg size (a gram/day).By showing 2-7 as can be known, day egg production was almost parallel between each was organized during 22 ages in week, showed as difference not remarkable (P＞0.05); 24,26 weeks were compared with the blank group during ages, and each group all shows trend of rising, and the I group is particularly evident, but difference not significantly (P＞0.05); When 28 ages in week, the I group exceeds blank group 5.47%, compares I group day egg production significant difference (P＜0.05) with the blank group.Day egg production and laying rate show similitude when the laying hen peak period.
The table 2-7 fruit of glossy privet is to the influence of laying hen day egg production
2-8 knows that the laying hen daily ingestion amount is in whole test phase process by table, and each organizes the trend that feed intake presents increase, but no significant difference (P＞0.05).Compare with the blank group, each processed group is showing as difference not remarkable (P＞0.05) 22,24,26 weeks; Whole test phase II group feed intake is all higher than other each group.Fruit of glossy privet II group feed intake maximum exceeds blank group 6.85% when 28 weeks, and wherein I group feed consumption is minimum, and the I group compares significant difference (P＜0.05) with the II group and other respectively organizes difference not remarkable (P＞0.05).
By table 3-4 as can be known, each organizes feedstuff-egg ratio in 22,24 differences in age in week not significantly (P＞0.05), wherein during 22 ages in week, compares with the blank group, and I, III group has descended 12.5%, 13.64% respectively, and II group and IV organize almost indifference of feedstuff-egg ratio; Respectively organize the feedstuff-egg ratio no significant difference during 24 ages in week; During 26 ages in week, compare with the blank group, the I group shows as otherness significantly (P＜0.05); Respectively organize difference not remarkable (P＞0.05) with other; The I group is significantly higher than other each processed group (P＜0.05) during 28 ages in week, indifference (P＞0.05) between other each group, and wherein the II group is compared with the blank group and is shown negative effect.It seems that totally the feedstuff-egg ratio of I group is minimum in the whole test phase, compares with the blank group, tests full phase feedstuff-egg ratio and significantly reduces (P＜0.05).
The table 2-9 fruit of glossy privet is to the influence of egg chick immune organ index
By table 2-10 as can be known, the immune same day, back 7 days fruit of glossy privet group antibody titers of immunity have trend of rising than control group and antibiotic group, but antibody titer does not have significant difference (P＞0.05) between each group.Back 14 days of immunity, fruit of glossy privet group antibody titer all is higher than control group, and wherein the antibody titer of 1% fruit of glossy privet group is significantly higher than other each groups (P＜0.05).Back 21 days of immunity, each test group antibody titer all is higher than control group, wherein 1% fruit of glossy privet group antibody titer and control group, 0.5% fruit of glossy privet group significant difference (P＜0.05).Back 28 days of immunity, each test group serum antibody titer all is higher than control group, and fruit of glossy privet group antibody titer is significantly higher than control group (P＜0.05).
The table 2-10 fruit of glossy privet is to the influence (log of inoculation ewcastle disease oil seepage egg chick serum antibody titer
2X)
Each is handled egg chick periphery lymphocyte conversion ratio and the results are shown in Table 2-11.After the ConA stimulation, fruit of glossy privet group lymphocyte conversion ratio is significantly higher than control group and antibiotic group (P＜0.05).
The table 2-11 fruit of glossy privet is to the influence of egg chick lymphocyte conversion ratio
The fruit of glossy privet sees Table 2-12 to each influence of handling laying hen different times serum immune globulin content.During 6 ages in week, each test group IgG content is significantly higher than control group (P＜0.05), IgA between each group, and IgM content does not have significant difference (P＞0.05); During 12 ages in week, 1% fruit of glossy privet group IgG content and control group significant difference (P＜0.05), each test group IgA content is compared no significant difference (P＞0.05) with control group, but IgM content is significantly higher than control group (P＜0.05); During 18 ages in week, the IgG of each test group, IgM content and control group difference are not remarkable, and the IgA content of 1% fruit of glossy privet group is significantly higher than control group (P＜0.05).
The table 2-12 fruit of glossy privet is to the influence (g/L) of IgG, IgA, IgM content in the serum
By table 2-13 as can be known, during 6 ages in week, add the fruit of glossy privet and can improve C in the serum
3Content, wherein 0.5% fruit of glossy privet group is than control group significant difference (P＜0.05), adds the fruit of glossy privet during 12 ages in week and can significantly improve C in the serum
3Content (P＜0.05), 18 weeks did not have significant difference (P＞0.05) between each group during ages, but the change of serum C of fruit of glossy privet group
3Content has the trend of raising than control group and antibiotic group.C in the fruit of glossy privet group different times serum
4Content all is higher than control group and antibiotic group, but does not have significant difference (P＞0.05).
The table 2-13 fruit of glossy privet is to egg chick change of serum C
3, C
4Influence (g/L)
By table 2-14 as can be known, during 6 ages in week, 1% fruit of glossy privet group IL-2 content is significantly higher than control group and antibiotic group (P＜0.05); During 12 ages in week, fruit of glossy privet group IL-2 content is significantly higher than control group and antibiotic group (P＜0.05), and is wherein the highest to add 1% fruit of glossy privet group IL-2 content; 18 weeks added the fruit of glossy privet and can significantly improve IL-2 content in the serum (P＜0.05) during ages, wherein 0.5% fruit of glossy privet group and control group difference extremely significantly (P＜0.01), 1% fruit of glossy privet and the blank significant difference (P＜0.05) of organizing.
The table 2-14 fruit of glossy privet is to the influence (ng/mL) of egg chick serum il-2 content
By table 2-15 as can be known, 6 ages in week and 18 weeks, TNF content all had increase than control group in each test group serum during ages, but difference not significantly (P＞0.05).During 12 ages in week, TNF content all is higher than control group in fruit of glossy privet group, the antibiotic group serum, wherein adds 1% fruit of glossy privet and can significantly improve TNF content in the serum (P＜0.05).
The table 2-15 fruit of glossy privet is to the influence (ng/mL) of egg chick serum TNF
Each processed group is to the bird flu H of laying hen
5N
1The hypotype antibody titer the results are shown in Table 2-16.Compare with the blank group, at immunity back 28 days or 42 days, the bird flu H of each test group
5The equal conspicuousness of hypotype antibody titer raises, and in immunity back 28 days, 0.5% fruit of glossy privet group antibody titer was significantly higher than blank group (P＜0.05); Back 42 days of immunity is respectively organized antibody titer and is still showed rising except that the performance of flavomycoin group antibody titer descends, 0.5% fruit of glossy privet is significantly higher than blank group (P＜0.05), and 1% fruit of glossy privet group, flavomycoin group and blank group are compared there was no significant difference (P＞0.05).
The table 2-16 fruit of glossy privet is to laying hen bird flu H
5N
1The influence of hypotype antibody titer
By table 2-17 as can be known 1% fruit of glossy privet group compare with the blank group, IgG and IgM show as significant difference (P＜0.05), wherein IgG compares blank group and exceeds between 33.3%, 0.5% fruit of glossy privet group and flavomycoin group and each group difference not remarkable (P＞0.05); Compare with the blank group, two kinds of fruit of glossy privet addition manners show as significant difference (P＜0.05) to the influence of IgA content, and flavomycoin group and each group difference is significantly (P＞0.05) not.
The table 2-17 fruit of glossy privet is to the influence of laying hen immunoglobulin (Ig)
Claims (1)
1. plant feed addictive method of improving immunity of layer chicken, it is characterized in that: described plant feed addictive is natural Chinese medicinal herb-fruit of glossy privet, its adding method is: the fruit of glossy privet is dried to constant weight through 65 ℃, pulverize, cross 40 mesh sieves, and add to respectively in the egg chick daily ration by 0.5%, 1% two dosage.
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| CN201010102523A CN101731480A (en) | 2010-01-29 | 2010-01-29 | Method for preparing vegetable feed additive for improving immunity of layer chicken |
| CN 201010165074 CN101861955A (en) | 2010-01-29 | 2010-04-12 | Preparation method of plant feed additive for improving immunity of layer chicken |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150758A (en) * | 2010-12-31 | 2011-08-17 | 东北农业大学 | Chinese herbal medicine compound feed for improving meat quality of table poultry and preparation method thereof |
| WO2013173972A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for growth in children |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103083414A (en) * | 2011-10-27 | 2013-05-08 | 天津生机集团股份有限公司 | Traditional Chinese medicine composition used for enhancing chicken immunity |
| CN103843721A (en) * | 2012-12-04 | 2014-06-11 | 秀山鲁渝禽业有限公司 | Breeding method for improving immunity of bred chicken |
| CN103689251A (en) * | 2013-11-26 | 2014-04-02 | 淮北正洋生物科技有限公司 | Selenium-rich feed for laying hens |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1596696A (en) * | 2004-08-18 | 2005-03-23 | 东北农业大学 | Natural vegetable immune promoting additive for feed |
| CN101411400A (en) * | 2008-12-04 | 2009-04-22 | 东北农业大学 | Glossy privet fruit extract for improving production performance and immunity function of laying hen and use method |
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2010
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150758A (en) * | 2010-12-31 | 2011-08-17 | 东北农业大学 | Chinese herbal medicine compound feed for improving meat quality of table poultry and preparation method thereof |
| WO2013173972A1 (en) * | 2012-05-22 | 2013-11-28 | Nestec S.A. | Fructus ligustri lucidi for growth in children |
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Open date: 20100616 |