CN101731450B - Preparation method of single cell protein feed by taking acetone butanol fermentation wastewater as raw materials - Google Patents
Preparation method of single cell protein feed by taking acetone butanol fermentation wastewater as raw materials Download PDFInfo
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Abstract
The invention relates to a preparation method of single cell protein feed by taking acetone butanol fermentation wastewater as raw materials, wherein acetone butanol fermentation wastewater is utilized to prepare fermentation medium; three-stage cultivation is carried out on the inclined-plane strains, then fermentation cultivation is carried out on inclined-plane strains and fermentation medium, and then single cell protein feed is prepared through separation, concentration, drying and pulverizing. In the invention, COD content in the wastewater is high, rich organic matter is good feed for preparing single cell protein feed, the production cost is low, and the invention can turn waste into wealth and has dramatic economic, environmental protection and social benefits.
Description
Technical field
The present invention relates to a kind of is the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water.
Background technology
At present, both at home and abroad acetone, butanols are mainly synthetic through petrochemical industry and since world petroleum resource in short supply, crude oil price rises significantly, the fermentative Production butanols becomes must becoming of future development.The domestic many enterprises of gesture begin or prepare to start, but the hysteresis of the treatment technology of acetone butanol fermentation waste water has become to restrict one of principal element of this industry development.Solvent in the final zymotic fluid of acetone butanol fermentation is at 1.6-2%; All the other 97～98% all are waste water; Produce great amount of wastewater behind the zymotic fluid process wine with dregs column distillation, one ton of solvent of every according to statistics production will produce the waste water about 50～60 tons, and this waste water is a kind of very typical industrial wastewater; Water quality characteristics be " two-supremes " promptly: high organic concentration, high suspended matter concentration.If directly discharging will be very huge to the harm that environment causes.Because the acetone butanol fermentation wastewater flow rate is big, the environmental protection treatment cost is high simultaneously, and this has greatly limited the development of acetone butanol fermentation industry.So how to realize that the resource of acetone butanol fermentation waste water is extremely urgent.At present, still do not utilize the report of the third fourth waste water manufacture order cell protein feed.
Summary of the invention
The purpose of this invention is to provide a kind of is the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water.
The technical scheme that the present invention adopts is:
A kind of is the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, may further comprise the steps:
A, get acetone-butanol distillation waste water; Adding mass fraction is the urea of 0.1-0.14%, and mass fraction is the industrial phosphoric acid of 0.03-0.08%, adds mass concentration again and is 98% the concentrated sulfuric acid pH value is transferred to 4.0-4.5; And sterilization 20-30min, obtain fermentation medium.
B, get the agar bevel culture medium that glucose that mass fraction is 1.5-2.5%, yeast extract that mass fraction is 0.2-0.8%, peptone that mass fraction is 0.5-1.5% and mass fraction are 1.5-2.5%.
C, choose bacterial classification and place slant medium, turn out slant strains.
D, first order seed are cultivated; The malt extract medium that with pol is 10-12 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; The slant strains of getting 1-3 ring is loaded in the triangular flask, is under 30 ℃ the condition in temperature, and placing the shaking table frequency is to cultivate 12-16h on the reciprocal shaking table of 100-120r/min.
E, secondary seed cultivate; get acetone-butanol distillation waste water, add mass fraction and be the sucrose of 0.3-0.5%, urea that mass fraction is 0.12-0.16% and mass fraction than be the industrial phosphoric acid of 0.03-0.08%, and to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.5-5.0 with pH value; obtain secondary seed medium; get 3L secondary seed nutrient solution and place the 5L serum bottle, treat the first order seed cultivation finish after in the access serum bottle, be to cultivate 12-16h under 30 ℃ the condition in temperature.
F, three grades of seed culture; The fermentation medium of getting among the 35L step a is loaded in the fermentation tank of 50L; Treat to be 28-30 ℃ in temperature in secondary seed cultivation the finishing back access fermentation tank, mixing speed is 100-110r/min; Throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 18-24h.
G, fermentation are got among fermentation medium and the f among the step a three grades and are cultivated seed liquor and place 1m
3Fermentation tank in, be 28-30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 18-24h of 1: 1.5 (v/v) obtains biomass and is>=8g/L, the mass fraction of crude protein is 42-52%, the waste water COD clearance is the zymotic fluid of 65-70%.
H, separation concentrate, and adopting disc-type yeast separation machine is the zymotic fluid of 10-12 ° of Brinx with zymotic fluid simmer down to pol.
I, drying and crushing, adopting double drum drier that the zymotic fluid among the step h is carried out dry baking temperature is 140-150, and pulverizes and make the grain fineness after the drying and crushing reach≤80 orders, water content is reduced to 8-10%, thereby makes finished product.
Said acetone-butanol distillation waste water is 28000-32000mg/L for acetone butanol fermentation liquid advanced the COD that the wine with dregs column distillation produced, and the reduced sugar mass fraction is 0.4-0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% waste water.
Acetone-butanol distillation waste water among the said step a is the waste water that filters through 80 mesh filter screens.
The mass fraction of the glucose among the said step b is 2%, and the mass fraction of yeast extract is 0.5%, and the mass fraction of peptone is 1%, and the mass fraction of agar is 2%.
Bacterial classification in the said steps d is at least a in candida utili (G.utilis), candida tropicalis (G.tropicalis) and the geotrichum candidum (Geotrichum candidum).
Acetone-butanol distillation waste water among the said step e is the waste water that filters through 80 keevil frames.
The mass fraction of urea is 0.12% among the said step a, and the mass fraction of industrial phosphoric acid is 0.05%.
The mass fraction of sucrose is 0.3% among the said step e, and the mass fraction of urea is 0.14%, and the mass fraction of industrial phosphoric acid is 0.05%.
Speed of agitator among the said step f is 100r/min.
Advantage of the present invention is: used waste water COD is higher, and containing abundant organic matter is the good raw material of fermenting and producing single-cell protein feed, and low production cost is turned waste into wealth, and remarkable economical, environmental protection and social benefit are arranged.
The specific embodiment
Embodiment 1
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 28000mg/L, and the reduced sugar mass fraction is 0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.1% urea; And mass fraction is 0.03% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.0, and sterilization 20min, fermentation medium obtained.
Get the glucose of 1.5% mass fraction, the yeast extract of 0.2% mass fraction, the peptone of 0.5% mass fraction and the agar bevel culture medium of 1.5% mass fraction.
Choose candida utili and place slant medium, turn out slant strains.
The malt extract medium that with pol is 10 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 1 ring candida utili slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 12h on the reciprocal shaking table of 100r/min to obtain one-level and cultivate seed.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.3% sucrose, mass fraction and be 0.12% urea and mass fraction and be 0.03% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.5 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 12h under 30 ℃ the condition to obtain secondary and cultivate seed in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 28 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 18h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 28 ℃ in temperature, speed of agitator is 90r/min, throughput be the condition bottom fermentation 18h of 1: 1.5 (v/v) to obtain biomass be 8g/L, crude protein quality mark 42%, waste water COD clearance are 65% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 10 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 140 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 8%.
Embodiment 2
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 32000mg/L, and the reduced sugar mass fraction is 0.4%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.14% urea; And mass fraction is 0.08% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.5, and sterilization 23min, fermentation medium obtained.
Get the glucose of 2.5% mass fraction, the yeast extract of 0.8% mass fraction, the peptone of 1.5% mass fraction and the agar bevel culture medium of 2.5% mass fraction.
Choose candida tropicalis and place slant medium, turn out slant strains.
The malt extract medium that with pol is 12 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 2 ring candida tropicalises slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 16h on the reciprocal shaking table of 120r/min to obtain one-level and cultivate seed.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.5% sucrose, mass fraction and be 0.16% urea and mass fraction and be 0.08% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 5.0 with the pH value; Obtain secondary seed medium, get 3L secondary seed nutrient solution and place the 5L serum bottle, treat that first order seed cultivated
Finishing the back and insert in the serum bottle, is to cultivate 16h under 30 ℃ the condition to obtain secondary and cultivate seed in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 110r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 24h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 24h of 1: 1.5 (v/v) obtains biomass 8.9g/L, crude protein quality mark 48%, waste water COD clearance are 67% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 12 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 150 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 10%.
Embodiment 3
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 30000mg/L, and the reduced sugar mass fraction is 0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.12% urea; And mass fraction is 0.05% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.3, and sterilization 20min, fermentation medium obtained.
Get the glucose of 2% mass fraction, the yeast extract of 0.5% mass fraction, the peptone of 1% mass fraction and the agar bevel culture medium of 2% mass fraction.
Choose geotrichum candidum and place on the slant medium, turn out slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 1 ring geotrichum candidum slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 13h on the reciprocal shaking table of 110r/min.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.4% sucrose, mass fraction and be 0.14% urea and mass fraction and be 0.05% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.7 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 29 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 21h.
Getting fermentation medium and three grades cultivates seed liquor and places 1m
3Fermentation tank in, be 29 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 21h of 1: 1.5 (v/v) obtains biomass 8g/L, crude protein quality mark 52%, waste water COD clearance are 70% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 11 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 145 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 9%.
Embodiment 4
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 29000mg/L, and the reduced sugar mass fraction is 0.45%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.12% urea; And mass fraction is 0.05% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.2, and sterilization 25min, fermentation medium obtained.
Get the glucose of 2.3% mass fraction, the yeast extract of 0.7% mass fraction, the peptone of 1.2% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida utili, candida tropicalis and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 10 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Get the 1 ring candida utili slant strains of turning out respectively and 1 ring candida tropicalis is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 13h on the reciprocal shaking table of 115r/min.
Get acetone-butanol distillation waste water; Add mass fraction and be 0.35% sucrose, mass fraction and be 0.14% urea and mass fraction and be 0.05% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.8 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 29 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 19h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 29 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 20h of 1: 1.5 (v/v) obtains biomass 8.3g/L, crude protein quality mark 43%, waste water COD clearance are 66% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 10 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 142 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 8%.
Embodiment 5
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 31000mg/L, and the reduced sugar mass fraction is 0.47%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.13% urea; And mass fraction is 0.04% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.0, and sterilization 22min, fermentation medium obtained.
Get the glucose of 2.1% mass fraction, the yeast extract of 0.3% mass fraction, the peptone of 0.8% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida tropicalis and geotrichum candidum and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the slant strains that 1 ring candida tropicalis slant strains of turning out and 1 ring geotrichum candidum turn out respectively is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 15h on the reciprocal shaking table of 110r/min.
Get acetone-butanol distillation waste water; Add mass fraction and be 0.45% sucrose, mass fraction and be 0.15% urea and mass fraction and be 0.04% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.8 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 20h.
Getting fermentation medium and three grades cultivates seed liquor and places 1m
3Fermentation tank in, be 30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 22h of 1: 1.5 (v/v) obtains biomass 8.7g/L, crude protein quality mark 44%, waste water COD clearance are 69% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 12 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 143 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 9%.
Embodiment 6
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 30000mg/L, and the reduced sugar mass fraction is 0.48%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% acetone-butanol distillation waste water; Filter through 80 mesh filter screens, get filtrating, the adding mass fraction is 0.13% urea; And mass fraction is 0.05% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.4, and sterilization 25min, fermentation medium obtained.
Get the glucose of 2.1% mass fraction, the yeast extract of 0.6% mass fraction, the peptone of 1.1% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida utili, candida tropicalis and geotrichum candidum and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the slant strains that slant strains that the 1 ring candida utili slant strains of turning out, 1 ring candida tropicalis turn out and 1 ring geotrichum candidum turn out respectively is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 15h on the reciprocal shaking table of 112r/min.
Get acetone-butanol distillation waste water; Add mass fraction and be 0.3% sucrose, mass fraction and be 0.13% urea and mass fraction and be 0.07% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.7 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 23h.
Getting fermentation medium and three grades cultivates seed liquor and places 1m
3Fermentation tank in, be 30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 22h of 1: 1.5 (v/v) obtains biomass 9.1g/L, crude protein quality mark 47%, waste water COD clearance are 65% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 11 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 147 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 8%.
Embodiment 7
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 28000mg/L; The reduced sugar mass fraction is 0.49%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.1% urea; And mass fraction is 0.03% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.0, and sterilization 30min, fermentation medium obtained.
Get the glucose of 1.5% mass fraction, the yeast extract of 0.2% mass fraction, the peptone of 0.5% mass fraction and the agar bevel culture medium of 1.5% mass fraction.
Choose candida utili and place slant medium, turn out slant strains.
The malt extract medium that with pol is 10 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 1 ring candida utili slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 12h on the reciprocal shaking table of 100r/min to obtain one-level and cultivate seed.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.3% sucrose, mass fraction and be 0.12% urea and mass fraction and be 0.03% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.5 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 12h under 30 ℃ the condition to obtain secondary and cultivate seed in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 28 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 18h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 28 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 18h of 1: 1.5 (v/v) obtains biomass 9.2g/L, crude protein quality mark 42%, waste water COD clearance are 65% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 10 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 140 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 8%.
Embodiment 8
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 32000mg/L; The reduced sugar mass fraction is 0.45%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.14% urea; And mass fraction is 0.08% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.5, and sterilization 30min, fermentation medium obtained.
Get the glucose of 2.5% mass fraction, the yeast extract of 0.8% mass fraction, the peptone of 1.5% mass fraction and the agar bevel culture medium of 2.5% mass fraction.
Choose candida tropicalis and place slant medium, turn out slant strains.
The malt extract medium that with pol is 12 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 2 ring candida tropicalises slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 16h on the reciprocal shaking table of 120r/min to obtain one-level and cultivate seed.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.5% sucrose, mass fraction and be 0.16% urea and mass fraction and be 0.08% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 5.0 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 16h under 30 ℃ the condition to obtain secondary and cultivate seed in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 110r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 24h.
Fermentation medium and three grades of cultivation seed liquor place 1m
3Fermentation tank in, be 30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 24h of 1: 1.5 (v/v) obtains biomass 8.3g/L, crude protein quality mark 52%, waste water COD clearance are 70% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 12 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 150 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 10%.
Embodiment 9
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 29000mg/L; The reduced sugar mass fraction is 0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.12% urea; And mass fraction is 0.05% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.3, and sterilization 30min, fermentation medium obtained.
Get the glucose of 2% mass fraction, the yeast extract of 0.5% mass fraction, the peptone of 1% mass fraction and the agar bevel culture medium of 2% mass fraction.
Choose geotrichum candidum and place on the slant medium, turn out slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the 2 ring geotrichum candidums slant strains of turning out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 13h on the reciprocal shaking table of 110r/min.
The acetone-butanol distillation waste water that the 80 keevil frames of learning from else's experience filter; Add mass fraction and be 0.4% sucrose, mass fraction and be 0.14% urea and mass fraction and be 0.05% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.7 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 29 ℃ in temperature, and mixing speed is 105r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 21h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 29 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 21h of 1: 1.5 (v/v) obtains biomass 8g/L, crude protein quality mark 44%, waste water COD clearance are 66% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 11 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 145 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 9%.
Embodiment 10
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 31000mg/L; The reduced sugar mass fraction is 0.45%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.11% urea; And mass fraction is 0.04% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.2, and sterilization 30min, fermentation medium obtained.
Get the glucose of 2.3% mass fraction, the yeast extract of 0.7% mass fraction, the peptone of 1.2% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida utili, candida tropicalis and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 10 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting 1 ring respectively gets the slant strains that slant strains that candida utili turns out and 1 ring candida tropicalis turn out and is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 13h on the reciprocal shaking table of 115r/min.
Get acetone-butanol distillation waste water; Add mass fraction and be 0.35% sucrose, mass fraction and be 0.13% urea and mass fraction and be 0.04% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.8 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 29 ℃ in temperature, and mixing speed is 110r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 20h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 29 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 22h of 1: 1.5 (v/v) obtains biomass 8.6g/L, crude protein quality mark 47%, waste water COD clearance are 67% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 10 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 141 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 8-10%.
Embodiment 11
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 30000mg/L; The reduced sugar mass fraction is 0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.13% urea; And mass fraction is 0.07% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.0, and sterilization 22min, fermentation medium obtained.
Get the glucose of 2.1% mass fraction, the yeast extract of 0.3% mass fraction, the peptone of 0.8% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida tropicalis and geotrichum candidum and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the slant strains that 1 ring candida tropicalis slant strains of turning out and 1 ring geotrichum candidum turn out is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 15h on the reciprocal shaking table of 110r/min.
Get acetone-butanol distillation waste water; Add mass fraction and be 0.45% sucrose, mass fraction and be 0.15% urea and mass fraction and be 0.06% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.8 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 100r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 22h.
Fermentation medium and three grades of cultivation seed liquor are placed 1m
3Fermentation tank in, be 30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 20h of 1: 1.5 (v/v) obtains biomass 9.2g/L, crude protein quality mark 46%, waste water COD clearance are 68% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 12 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out drying, baking temperature is 148 ℃, pulverizes to make the fineness after the drying and crushing reach 80 orders, and water content is reduced to 9%.
Embodiment 12
Getting acetone butanol fermentation liquid, to advance the COD that the wine with dregs column distillation produced be 30000mg/L; The reduced sugar mass fraction is 0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent distills waste water less than 0.02% acetone-butanol, and the adding mass fraction is 0.13% urea; And mass fraction is 0.06% industrial phosphoric acid; Add mass concentration again and be 98% the concentrated sulfuric acid pH value is transferred to 4.4, and sterilization 23min, fermentation medium obtained.
Get the glucose of 2.1% mass fraction, the yeast extract of 0.6% mass fraction, the peptone of 1.1% mass fraction and the agar bevel culture medium of 2.2% mass fraction.
Choose candida utili, candida tropicalis and geotrichum candidum and place slant medium respectively, turn out corresponding slant strains.
The malt extract medium that with pol is 11 ° of Brinx is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; Getting the slant strains that slant strains that the 1 ring candida utili slant strains of turning out, 1 ring candida tropicalis turn out and 1 ring geotrichum candidum turn out respectively is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 15h on the reciprocal shaking table of 112r/min.
The learnt from else's experience acetone-butanol distillation waste water of plate-frame filtering; Add mass fraction and be 0.3% sucrose, mass fraction and be 0.15% urea and mass fraction and be 0.07% industrial phosphoric acid; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.7 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 14h under 30 ℃ the condition in temperature.
Getting in the fermentation tank that the 35L fermentation medium is loaded on 50L, treat that secondary seed cultivates the back that finishes and insert in the fermentation tank, is 30 ℃ in temperature, and mixing speed is 105r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 23h.
Getting fermentation medium and three grades cultivates seed liquor and places 1m
3Fermentation tank in, be 28 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 19h of 1: 1.5 (v/v) obtains biomass 9.1g/L, crude protein quality mark 47%, waste water COD clearance are 69% zymotic fluid.
Adopting disc-type yeast separation machine is the zymotic fluid of 11 ° of Brinx with zymotic fluid simmer down to pol.
Adopt double drum drier to carry out the feasible drying of drying and crushing, baking temperature is that 146 ℃ of fineness after the pulverizing reach 80 orders, and water content is reduced to 8.5%.
Claims (8)
1. one kind is the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, it is characterized in that, may further comprise the steps:
A, get acetone-butanol distillation waste water; Adding mass fraction is the urea of 0.1-0.14%, and mass fraction is the industrial phosphoric acid of 0.03-0.08%, adds mass concentration again and is 98% the concentrated sulfuric acid pH value is transferred to 4.0-4.5; And sterilization 20-30min, obtain fermentation medium;
B, get the agar bevel culture medium that glucose that mass fraction is 1.5-2.5%, yeast extract that mass fraction is 0.2-0.8%, peptone that mass fraction is 0.5-1.5% and mass fraction are 1.5-2.5%;
C, choose bacterial classification and place slant medium, turn out slant strains, said bacterial classification is at least a in candida utili, candida tropicalis and the geotrichum candidum;
D, first order seed are cultivated: the malt extract medium that with pol is 10-12 ° of Brix is as the first order seed culture medium; And get in the triangular flask that 100mL is loaded on 500mL; The slant strains of getting the 1-3 ring is loaded in the triangular flask; Be under 30 ℃ the condition in temperature, placing the shaking table frequency is to cultivate 12-16h on the reciprocal shaking table of 100-120r/min;
E, secondary seed are cultivated: get acetone-butanol distillation waste water; Add mass fraction and be the sucrose of 0.3-0.5%, urea that mass fraction is 0.12-0.16% and mass fraction than being the industrial phosphoric acid of 0.03-0.08%; And to use mass concentration be that 98% the concentrated sulfuric acid is transferred to 4.5-5.0 with the pH value, obtains secondary seed medium, gets 3L secondary seed nutrient solution and place the 5L serum bottle; Treating that first order seed cultivates the back that finishes and insert in the serum bottle, is to cultivate 12-16h under 30 ℃ the condition in temperature;
F, three grades of seed culture: the fermentation medium of getting among the 35L step a is loaded in the fermentation tank of 50L; Treat in secondary seed cultivation the finishing back access fermentation tank; In temperature is 28-30 ℃; Mixing speed is 100-110r/min, and throughput is to carry out three grades of seed culture under the condition of 1: 1.5 (v/v), and incubation time is 18-24h;
G, fermentation: get among fermentation medium and the step f among the step a three grades and cultivate seed liquor and place 1m
3Fermentation tank in, be 28-30 ℃ in temperature, speed of agitator is 90r/min, throughput is that the condition bottom fermentation 18-24h of 1: 1.5 (v/v) obtains biomass and is>=8g/L, the mass fraction of crude protein is 42-52%, the waste water COD clearance is the zymotic fluid of 65-70%;
H, separation concentrate: adopting disc-type yeast separation machine is the zymotic fluid of 10-12 ° of Brix with zymotic fluid simmer down to pol;
I, drying and crushing: adopt double drum drier that the zymotic fluid among the step h is carried out drying, baking temperature is 140-150 ℃, and pulverizes and make the grain fineness after the drying and crushing reach≤80 orders, and water content is reduced to 8-10%, thereby makes finished product.
2. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water; It is characterized in that: said acetone-butanol distillation waste water is 28000-32000mg/L for acetone butanol fermentation liquid advanced the COD that the wine with dregs column distillation produced; The reduced sugar mass fraction is 0.4-0.5%, and the mass fraction of n-butanol, acetone, ethanol three total solvent is less than 0.02% waste water.
3. according to claim 1 and 2 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, it is characterized in that: the waste water of acetone-butanol among said step a distillation waste water for filtering through 80 mesh filter screens.
4. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water; It is characterized in that: the mass fraction of the glucose among the said step b is 2%; The mass fraction of yeast extract is 0.5%; The mass fraction of peptone is 1%, and the mass fraction of agar is 2%.
5. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, it is characterized in that: the waste water of acetone-butanol among said step e distillation waste water for filtering through 80 keevil frames.
6. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, it is characterized in that: the mass fraction of urea is 0.12% among the said step a, the mass fraction of industrial phosphoric acid is 0.05%.
7. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water; It is characterized in that: the mass fraction of sucrose is 0.3% among the said step e; The mass fraction of urea is 0.14%, and the mass fraction of industrial phosphoric acid is 0.05%.
8. according to claim 1 a kind of be the preparation method of raw material production single-cell protein feed with acetone butanol fermentation waste water, it is characterized in that: the speed of agitator among the said step f is 100r/min.
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| CN102030417A (en) * | 2010-11-01 | 2011-04-27 | 黄虹寓 | Method for performing hydrolytic treatment on waste liquid during production of biofuel ethanol |
| CN104108763B (en) * | 2014-07-01 | 2015-12-02 | 中国科学院广州能源研究所 | A kind of biological butanol fermentation waste water process co-production organic acid method |
| CN109370928A (en) * | 2018-11-20 | 2019-02-22 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of candida utili and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125058A (en) * | 1994-12-22 | 1996-06-26 | 梁兴中 | Method for preparing solid protein feedstuff from sacchariferous fermented waste liquor |
| CN1172593A (en) * | 1997-08-27 | 1998-02-11 | 冯东勋 | Method for producing protein feed by fermenting the waste water of sodium glutomate |
| CN1299605A (en) * | 1999-12-15 | 2001-06-20 | 李红光 | Concentration treatment of alcohol or monosodium glutamate containing waste water to produce fermented feed |
| CN1437864A (en) * | 2003-01-28 | 2003-08-27 | 金新梅 | Process of making high-concentration waste liquid of aginomoto convert into fodder protein |
| CN1628542A (en) * | 2003-12-18 | 2005-06-22 | 李红光 | New energy-saving technique for producing albumen feed by drying concentrated solution of wastewater from fermentation industry |
| CN101302545A (en) * | 2008-04-29 | 2008-11-12 | 江苏联海生物科技有限公司 | Energy-saving production method of acetone-butanol |
-
2009
- 2009-12-21 CN CN2009102643546A patent/CN101731450B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1125058A (en) * | 1994-12-22 | 1996-06-26 | 梁兴中 | Method for preparing solid protein feedstuff from sacchariferous fermented waste liquor |
| CN1172593A (en) * | 1997-08-27 | 1998-02-11 | 冯东勋 | Method for producing protein feed by fermenting the waste water of sodium glutomate |
| CN1299605A (en) * | 1999-12-15 | 2001-06-20 | 李红光 | Concentration treatment of alcohol or monosodium glutamate containing waste water to produce fermented feed |
| CN1437864A (en) * | 2003-01-28 | 2003-08-27 | 金新梅 | Process of making high-concentration waste liquid of aginomoto convert into fodder protein |
| CN1628542A (en) * | 2003-12-18 | 2005-06-22 | 李红光 | New energy-saving technique for producing albumen feed by drying concentrated solution of wastewater from fermentation industry |
| CN101302545A (en) * | 2008-04-29 | 2008-11-12 | 江苏联海生物科技有限公司 | Energy-saving production method of acetone-butanol |
Non-Patent Citations (1)
| Title |
|---|
| 李灵巧等.酵母法处理丙酮-丁醇发酵废水研究.《工业水处理》.2008,第28卷(第11期),56-58. * |
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