CN101731446A - Whey protein powder prepared by taking milk as raw material and preparation method thereof - Google Patents
Whey protein powder prepared by taking milk as raw material and preparation method thereof Download PDFInfo
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- CN101731446A CN101731446A CN200910261767A CN200910261767A CN101731446A CN 101731446 A CN101731446 A CN 101731446A CN 200910261767 A CN200910261767 A CN 200910261767A CN 200910261767 A CN200910261767 A CN 200910261767A CN 101731446 A CN101731446 A CN 101731446A
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Abstract
The invention relates to the field of powered milk, in particular relating to whey protein powder by taking milk as a raw material and a preparation method thereof. The method of the invention comprises the following steps: 1) microfiltration: leading milk or reduced milk to pass through a filtration membrane of 0.1mu m; 2) ultrafiltration: leading milk passing through the microfiltration membrane to pass through the ultrafiltration membrane, wherein the interceptor of the filtration membrane is the whey protein; and 3) carrying out concentration, sterilization and spray drying on the whey protein liquid incepted by the filtration membrane to obtain the whey protein powder. In the method of the invention, soluble whey protein is directly extracted from fresh milk through advanced membrane techniques such as low temperature, microfiltration, ultrafiltration and the like, thus maintaining protein activity for the most part, having higher nutrition value, removing lactose, and containing no additives and residual enzyme. The specific value of tryptophane to long-chain neutral amino acid in whey protein powder prepared by the method in the invention is higher than the specific value of ordinary WPC/WPI, as the whey protein in the invention has higher content of alpha-lactoalbumin and does not contain glycomacropeptide.
Description
Technical field
The present invention relates to the milk powder field, particularly, the present invention relates to a kind of is the PURE WHEY and preparation method thereof of feedstock production with milk.
Background technology
In order to satisfy infant's nutritional need, the essential amino acids content of Infant Formula Enterprises and proportioning are good more near breast milk more, contain a large amount of tryptophans in the breast milk, the effect that tryptophan and threonine play in the infant growth process is also inequality, so the height of tryptophan and threonine content and ratio have certain influence to infant's brain development.
Need to add lactalbumin in the raw material of baby milk, the production method of existing lactalbumin is with the remaining whey liquid of processed cheese galactopoiesis in next life albumin, wherein contains a large amount of PROVON 190s and lactose.PROVON 190 is that milk coagulating enzyme action produces in the k-casein in the cheese production process, contains the threonine of 11-12% in the PROVON 190.So just cause adding in the babies ' formula milk powder of above-mentioned lactalbumin and contain a large amount of threonines, and high levels of threonine can cause amion acetic acid content in the brain increase the balance of the mediator that affects the nerves.And, the lactalbumin inanimate object activity of existing method preparation, contain additive, lactose, residual enzyme etc.
Summary of the invention
To provide a kind of be the method for feedstock production PURE WHEY with milk in order to address the above problem in the present invention.
Still a further object of the present invention provides the PURE WHEY by method for preparing.
According to of the present invention be that the method for feedstock production PURE WHEY may further comprise the steps with milk:
1) micro-filtration, with milk or recombined milk by 0.1 micron filter membrane, thereby bacterium, fat, casein micelle are blocked, the milk dissolubility filter membrane that communicates;
2) ultrafiltration will be carried out ultrafiltration by the milk of micro-filtration membrane, and the filter membrane interceptor is lactalbumin, and lactose, water, soluble-salt, nonprotein nitrogen pass through filter membrane;
3) the lactalbumin liquid of being tackled by milipore filter gets the lactoalbumin powder by the concentrating sterilizing spray-drying, and wherein, sterilization temperature is 87～93 ℃, and technology all is controlled at 4-10 ℃ before the concentrating sterilizing spray-drying, uses vavuum pump to drive feed liquid and flows.
According to of the present invention be the method for feedstock production PURE WHEY with milk, wherein, the parameter of microfiltration process is: feed pressure 2.0-2.5bar, circulating pressure 3.0-6.0bar, dialysate pressure 1.0-5.0bar, rate of circulating flow 3000-6000l/h, feeding temperature 6-10 ℃, circulating temperature 6-10 ℃, dialysis flow velocity 50-200l/h.
According to of the present invention be the method for feedstock production PURE WHEY with milk, wherein, the parameter of ultra-filtration process is: feed pressure 1.0-4.0bar, circulating pressure 3.0-6.0bar, dialysate pressure 1.0-5.0bar, rate of circulating flow 3000-6000l/h, feeding temperature 6-10 ℃, circulating temperature 6-10 ℃, dialysis flow velocity 50-200l/h.
The present invention also provides the PURE WHEY by method for preparing.
The present invention also provides the baby milk of the PURE WHEY that contains above-mentioned preparation.
Method of the present invention is directly extracted the lactalbumin of solubility from fresh milk through advanced membrane technologies such as low temperature, micro-filtration and ultrafiltration, kept protein active largely, have more high nutritive value, and removed lactose, do not contain any additives, residual enzyme.Be higher than the ratio of general WPC (WPC)/WPI (whey isolate protein) by the ratio of tryptophan in the PURE WHEY of method of the present invention preparation and long-chain neutral amino acid, this is by the ALA that contains high-load more in the lactalbumin of the present invention and does not contain PROVON 190.And the WPC/WPI that extracts from cheese whey contains PROVON 190, contains the threonine of 11-12% in the PROVON 190.In addition, the baby milk that contains PURE WHEY of the present invention can reduce protein content in the formula milk, and keep necessary tryptophane simultaneously, make nutritional labeling in the baby formula more near the breast milk level, to compare cost lower with the WPC prescription that uses WPC or strengthen alpha-lactalbumin.
The specific embodiment
Below describe technology of the present invention and characteristics in detail by specific embodiment, but these embodiment are not in order to limit protection scope of the present invention.
The selection of each raw material all meets the concerned countries standard among the following embodiment.
Embodiment 1
The production method of the milk powder of present embodiment mainly may further comprise the steps:
1, raw material milk (former milk, raw material milk) acceptance inspeciton, clean breast: require fat, albumen, dry, freshness and other indexs all to reach the requirement of raw material milk quality standard, and remove mechanical admixture in the raw material milk and standardization as required by clean newborn technology;
2, by plate type heat exchanger, milk is cooled to below 10 ℃.
3, carry out ultrafiltration mutually by (by 0.1 micron filter membrane) micro-filtration, milk dissolubility, the parameter of wherein said microfiltration process is: feed pressure 2.0bar, circulating pressure 3.0bar, dialysate pressure 1.0bar, rate of circulating flow 3000l/h, 6 ℃ of feeding temperatures, 6 ℃ of circulating temperatures, dialysis flow velocity 50l/h.The parameter of described ultra-filtration process is: feed pressure 1.0bar, circulating pressure 3.0bar, dialysate pressure 1.0bar, rate of circulating flow 3000l/h, 6 ℃ of feeding temperatures, 6 ℃ of circulating temperatures, dialysis flow velocity 50l/h.
4, the lactalbumin material of being tackled by filter membrane carries out the flash distillation sterilization and concentrates discharging after the concentration of material reaches 11～22 Baume degrees; The concrete parameter of this step is as follows:
Concentrate parameter: the flash distillation sterilization temperature is controlled at 87～93 ℃, owing to contain a large amount of albumen in the batching, temperature control is extremely important, among the present invention temperature is controlled at 87～93 ℃, can effectively prevent the too high generation albuminous degeneration of temperature when reaching good bactericidal effect.
Use equipment: a cover triple effect downward film evaporator, feed liquid is carried out sterilization and concentrated through feed pump to concentration section, refluxes in the compensating groove and filters with a yarn bag.Key points for operation:
(a) concentration of discharging for the first time should be controlled at the lower limit of concentration range, is easy to adjust after workshop section starts shooting for guaranteeing to dust;
(b) technological parameter:
88～92 ℃ of sterilization temperatures (summer); Steam pressure 0.3～0.4MPa;
Vacuum ranges (MPa):
I is imitated separator 0.055～0.075, and I is imitated heater 0.040～0.065
II is imitated separator 0.055～0.080, and II is imitated heater 0.050～0.075
III is imitated separator 0.070～0.080, and III is imitated heater 0.070～0.080
Mixing condenser vacuum ranges 0.080～0.090;
Temperature range (℃):
I is imitated separator 60～70, and I is imitated heater 65～80
II is imitated separator 50～70, and II is imitated heater 55～70
III is imitated separator 40～50, and III is imitated heater 45～70;
Discharging concentration (Baume degrees): 11～22;
5, the dense feed liquid that obtains after concentrating is carried out spray-drying, and wherein, the parameter of dusting is as follows: equipment 500 type vertical pressure spray drying towers have three spray gun cat head agglomeration and secondary fluidized bed plant.Dense milk enters through three spray guns through milk pump, high-pressure pump and carries out drying in the tower, and wherein EAT is between 160-180 ℃, and temperature of outgoing air is 88-95 ℃, between the interior negative pressure 30-80pa of tower, and high-pressure pump pressure 10-20Mpa.
6, in order to guarantee the standardization of production operation, set up special quality monitoring personnel, guarantee strict established standard and the parameter carried out of each operation.
7, in order to ensure security of products, in process of production, in strict accordance with the way to manage of HACCP
Embodiment 2
The parameter of described microfiltration process is: feed pressure 2.5bar, circulating pressure 6.0bar, dialysate pressure 5.0bar, rate of circulating flow 6000l/h, 10 ℃ of feeding temperatures, 10 ℃ of circulating temperatures, dialysis flow velocity 200l/h.The parameter of ultra-filtration process is: feed pressure 4.0bar, circulating pressure 6.0bar, dialysate pressure 5.0bar, rate of circulating flow 6000l/h, 10 ℃ of feeding temperatures, 10 ℃ of circulating temperatures, dialysis flow velocity 200l/h.
Other step is with embodiment 1.
Embodiment 3
The parameter of described microfiltration process is: feed pressure 2.2bar, circulating pressure 4.0bar, dialysate pressure 3.0bar, rate of circulating flow 5000l/h, 810 ℃ of feeding temperatures, 810 ℃ of circulating temperatures, dialysis flow velocity 150l/h.The parameter of ultra-filtration process is: feed pressure 2.5bar, circulating pressure 4.0bar, dialysate pressure 4.0bar, rate of circulating flow 4500l/h, 8 ℃ of feeding temperatures, 8 ℃ of circulating temperatures, dialysis flow velocity 150l/h.
Other step is with embodiment 1.
The PURE WHEY of embodiment 1-3 preparation and the contrast of existing WPC/WPI characteristic are as shown in the following Table 1.
Table 1
| The project contrast | Existing WPC/WPI product | Embodiment 1-3 |
| Biologically active | Do not have | Have |
| Albuminous degeneration may | Bigger | Do not have |
| Stable milk constituents | Do not have | Have |
| Activity of residual enzyme and residual dead bacterium thalline | Have | Do not have |
| The cheese derived peptide | Very high | Do not have |
The contrast of soluble protein and tryptophane as shown in the following Table 2 among product of the present invention and the WPC/WPI.
Table 2
| The project contrast | Embodiment 1-3 | Existing WPC/WPI product |
| Aspartic acid | ??11.5 | ??10.45 |
| Threonine | ??5.1 | ??6.7 |
| Serine | ??4.8 | ??5.25 |
| Glutamic acid | ??16.6 | ??17.1 |
| Proline | ??4.6 | ??6.2 |
| Amion acetic acid | ??2.1 | ??2.05 |
| Alanine | ??4.8 | ??4.95 |
| Valine | ??5.2 | ??6.1 |
| Methionine | ??2.1 | ??2.1 |
| The I isoleucine | ??5.0 | ??5.6 |
| Leucine | ??12.0 | ??10.4 |
| Tyrosine | ??3.2 | ??2.8 |
| Phenylalanine | ??3.7 | ??3.3 |
| Histidine | ??2.0 | ??1.8 |
| Lysine | ??9.7 | ??8.3 |
| Arginine | ??2.4 | ??2.7 |
| Tryptophan | ??2.2 | ??1.55 |
| Cystine | ??3.0 | ??2.6 |
| Tryptophan/LNAA | ??7.6 | ??5.5 |
The ratio of tryptophan in the product of the present invention and long-chain neutral amino acid is higher than general WPC/WPI, and this is by the ALA that contains high-load more in this product and does not contain PROVON 190.The WPC/WPI that extracts from cheese whey contains PROVON 190, contains the threonine (PROVON 190 is that milk coagulating enzyme action produces in the k-casein in the cheese production process) of 11-12% in the PROVON 190.Use product of the present invention can reduce the protein content in the formula milk and keep necessary tryptophane simultaneously, make nutritional labeling in the baby formula more near the breast milk level, to compare cost lower with the WPC prescription that uses WPC or strengthen alpha-lactalbumin.
The contrast of tryptophan and threonine ratio as shown in the following Table 3 in lactalbumin of the present invention and the existing whey protein product.
Table 3
| ??g/100g(Pro) | The present invention (embodiment 1-3) | The tradition lactalbumin | Soybean | Breast milk |
| ??Trp | ??2.07 | ??1.81 | ??1.27 | ??2.34 |
| ??Thr | ??5.04 | ??6.98 | ??3.56 | ??5.29 |
| ??Trp/Thr?Ratio | ??0.41 | ??0.26 | ??0.36 | ??0.41 |
With tryptophan and threonine ratio in table 3 lactalbumin of the present invention as can be seen near breast milk.
Claims (5)
1. one kind is the method for feedstock production PURE WHEY with milk, it is characterized in that, said method comprising the steps of:
1) micro-filtration passes through 0.1 micron filter membrane with milk;
2) ultrafiltration will be carried out ultrafiltration by the milk of micro-filtration membrane, and the interceptor after the ultrafiltration is lactalbumin;
3) the above-mentioned lactalbumin that obtains is made the lactoalbumin powder by the concentrating sterilizing spray-drying, wherein, sterilization temperature is 87～93 ℃.
2. according to claim 1 is the method for feedstock production PURE WHEY with milk, it is characterized in that the parameter of described microfiltration process is: feed pressure 2.0-2.5bar, circulating pressure 3.0-6.0bar, dialysate pressure 1.0-5.0bar, rate of circulating flow 3000-6000l/h, feeding temperature 6-10 ℃, circulating temperature 6-10 ℃, dialysis flow velocity 50-200l/h.
3. according to claim 1 is the method for feedstock production PURE WHEY with milk, it is characterized in that the parameter of ultra-filtration process is: feed pressure 1.0-4.0bar, circulating pressure 3.0-6.0bar, dialysate pressure 1.0-5.0bar, rate of circulating flow 3000-6000l/h, feeding temperature 6-10 ℃, circulating temperature 6-10 ℃, dialysis flow velocity 50-200l/h.
4. a PURE WHEY is characterized in that, the method preparation of described PURE WHEY by may further comprise the steps,
1) micro-filtration passes through 0.1 micron filter membrane with milk;
2) ultrafiltration will be carried out ultrafiltration by the milk of micro-filtration membrane, and the interceptor after the ultrafiltration is lactalbumin;
3) the above-mentioned lactalbumin that obtains is made the lactoalbumin powder by the concentrating sterilizing spray-drying, wherein, sterilization temperature is 87～93 ℃.
5. a baby milk is characterized in that, described baby milk comprises the described PURE WHEY of claim 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910261767A CN101731446A (en) | 2009-12-29 | 2009-12-29 | Whey protein powder prepared by taking milk as raw material and preparation method thereof |
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|---|---|---|---|
| CN200910261767A CN101731446A (en) | 2009-12-29 | 2009-12-29 | Whey protein powder prepared by taking milk as raw material and preparation method thereof |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102475151A (en) * | 2010-11-25 | 2012-05-30 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method for producing whey beverages |
| CN102511558A (en) * | 2011-12-29 | 2012-06-27 | 内蒙古伊利实业集团股份有限公司 | Liquid milk applicable to babies 6-12 months old and production method of liquid milk |
| CN104186916A (en) * | 2014-08-06 | 2014-12-10 | 中国科学院新疆理化技术研究所 | Method for separating and preparing whey protein powder from whey |
| CN105341316A (en) * | 2015-12-16 | 2016-02-24 | 新希望双喜乳业（苏州）有限公司 | Method for extracting high-purity whey protein from milk |
| CN105410328A (en) * | 2015-12-16 | 2016-03-23 | 新希望双喜乳业（苏州）有限公司 | Method for extracting whey protein powder from milk |
| CN106234754A (en) * | 2015-06-13 | 2016-12-21 | Dmk德意志牛奶股份有限公司 | The method producing aseptic Lactalbumin concentrate |
| CN106417888A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein |
| CN108432881A (en) * | 2018-03-16 | 2018-08-24 | 齐兴悦 | A kind of Moringa double protein drink breast prescription and preparation method thereof |
| CN109169911A (en) * | 2018-09-19 | 2019-01-11 | 成都连接流体分离科技有限公司 | A kind of new process producing highly active protein, low-lactose milk |
| CN109527115A (en) * | 2018-10-18 | 2019-03-29 | 中国农业科学院农产品加工研究所 | Sheep whey powder and preparation method thereof |
| CN109938102A (en) * | 2019-04-29 | 2019-06-28 | 飞潮（无锡）过滤技术有限公司 | A kind of film integrated treatment process of milk protein classification |
| CN110663773A (en) * | 2019-01-08 | 2020-01-10 | 东北农业大学 | Method for separating natural whey protein from fresh milk and application |
| CN113519626A (en) * | 2021-07-15 | 2021-10-22 | 青岛圣桐营养食品有限公司 | Lactose-free milk powder and preparation method thereof |
| CN113519627A (en) * | 2021-07-15 | 2021-10-22 | 青岛圣桐营养食品有限公司 | Lactose-free milk powder with low browning |
-
2009
- 2009-12-29 CN CN200910261767A patent/CN101731446A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102475151A (en) * | 2010-11-25 | 2012-05-30 | 内蒙古蒙牛乳业（集团）股份有限公司 | Method for producing whey beverages |
| CN102511558A (en) * | 2011-12-29 | 2012-06-27 | 内蒙古伊利实业集团股份有限公司 | Liquid milk applicable to babies 6-12 months old and production method of liquid milk |
| CN104186916A (en) * | 2014-08-06 | 2014-12-10 | 中国科学院新疆理化技术研究所 | Method for separating and preparing whey protein powder from whey |
| CN104186916B (en) * | 2014-08-06 | 2016-08-31 | 中国科学院新疆理化技术研究所 | The method that lactalbumin powder is prepared in separation from milk surum |
| CN106234754B (en) * | 2015-06-13 | 2021-09-28 | Dmk德意志牛奶股份有限公司 | Method for producing sterile whey protein concentrate |
| CN106234754A (en) * | 2015-06-13 | 2016-12-21 | Dmk德意志牛奶股份有限公司 | The method producing aseptic Lactalbumin concentrate |
| CN105341316A (en) * | 2015-12-16 | 2016-02-24 | 新希望双喜乳业（苏州）有限公司 | Method for extracting high-purity whey protein from milk |
| CN105410328A (en) * | 2015-12-16 | 2016-03-23 | 新希望双喜乳业（苏州）有限公司 | Method for extracting whey protein powder from milk |
| CN106417888A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Method for separating cow milk beta-casein and whey protein at low temperature through microfiltration to simulate composition of human lactoprotein |
| CN108432881A (en) * | 2018-03-16 | 2018-08-24 | 齐兴悦 | A kind of Moringa double protein drink breast prescription and preparation method thereof |
| CN109169911A (en) * | 2018-09-19 | 2019-01-11 | 成都连接流体分离科技有限公司 | A kind of new process producing highly active protein, low-lactose milk |
| CN109527115A (en) * | 2018-10-18 | 2019-03-29 | 中国农业科学院农产品加工研究所 | Sheep whey powder and preparation method thereof |
| CN109527115B (en) * | 2018-10-18 | 2022-03-08 | 中国农业科学院农产品加工研究所 | Sheep whey powder and preparation method thereof |
| CN110663773A (en) * | 2019-01-08 | 2020-01-10 | 东北农业大学 | Method for separating natural whey protein from fresh milk and application |
| CN109938102A (en) * | 2019-04-29 | 2019-06-28 | 飞潮（无锡）过滤技术有限公司 | A kind of film integrated treatment process of milk protein classification |
| CN113519626A (en) * | 2021-07-15 | 2021-10-22 | 青岛圣桐营养食品有限公司 | Lactose-free milk powder and preparation method thereof |
| CN113519627A (en) * | 2021-07-15 | 2021-10-22 | 青岛圣桐营养食品有限公司 | Lactose-free milk powder with low browning |
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Application publication date: 20100616 |