CN101726486B - Quick analyzing method for glutelin content of wheat - Google Patents
Quick analyzing method for glutelin content of wheat Download PDFInfo
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- CN101726486B CN101726486B CN2009102327695A CN200910232769A CN101726486B CN 101726486 B CN101726486 B CN 101726486B CN 2009102327695 A CN2009102327695 A CN 2009102327695A CN 200910232769 A CN200910232769 A CN 200910232769A CN 101726486 B CN101726486 B CN 101726486B
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Abstract
The invention relates to a quick analyzing method for glutelin content of wheat. 5 to 30 grams of representative kernels is selected from a wheat sample to be tested, the kernels are milled into whole powder by a laboratory mill, the powder is screened by a screen of which aperture is 0.5 millimeter, then the screened powder is filled in a plastic self-sealing bag, the powder is stood for at least 2 to 4 hours at the room temperature to balance the moisture, then the moisture content is measured, and the powder is used as a tested sample. The method is characterized by comprising the following steps: accurately weighing 100 milligrams of the tested sample, putting the tested sample in a centrifugal tube, separately extracting monomer protein from the tested sample by a solvent, or extracting monomer protein and soluble glutelin polymer with lower molecular weight at the same time, and then removing supernate after centrifugation to keep the glutelin in the centrifugal tube; and adding 1 milliliter of biuret reagent into the centrifugal tube, treating the mixture by using an ultrasonic crusher, and measuring the content of the glutelin in the tested sample through the color development reaction of glutelin components, wherein the content of the glutelin in the tested sample refers to the total amount of the glutelin in the tested sample or the content of large glutelin polymer.
Description
Technical field
The present invention relates to the separation and quantitative method of wheat gluten, belong to the cereal chemistry field.
Background technology
Protein accounts for 8%～20% of wheat seed weight, mainly is made up of glutelin (Glutenin) and two types of storage proteins of alcohol soluble protein (Gliadin).Wherein glutelin is the major influence factors of wheat gluten quality and food quality, is most important objective trait in the Wheat Quality Improvement, is used as the important content of wheat grain chemical research always.In the wheat gluten quality assessment, early many from generation to generation use protein contents, sedimentation value and hmw glutenin subunit mark are selected; Advanced lines uses protein content and dough rheological characteristics more; Basically the General Principle that then has a good gluten quality according to the genotype with high protein, high quality subunit is selected; Bring into play vital role at China's Wheat Breeding for Quality initial stage, improved wheat processing products quality greatly.But, find that more and more those genotype with high protein, high quality subunit not necessarily have good processing quality along with the raising of quality breeding level.Wheat gluten exists with the form of condensate (Glutenin polymer), and wherein extent of polymerization is higher, and the part that is difficult for being extracted is called the big aggressiveness of glutelin, and (GMP), there is significant forward correlativity in Glutenin macro polymer with gluten strength.Therefore, glutelin condensate total amount, the big aggressiveness content of glutelin are the important contents of wheat breeding quality evaluation in the generation.
The assay method of current glutelin content mainly contains following several kinds:
One, biuret colourimetry
The biuret colourimetry is simple to operate, quick, is the common method of protein content determination.This method is through peptide bond and Cu under alkali condition
2+Chromogenic reaction protein content is measured, be suitable for the protein content determination of all kinds, have characteristics simple to operate, quick, with low cost.The biuret colourimetry is existing (Liu Li, Zhouyang, the He Zhonghu of using in glutelin content is measured;
R.J.; Zhang Liping .Glu-1 and Glu-3 allelic variation are to the influence of insoluble glutelin content. Acta Agronomica Sinica, and 2004,30:1086-1092); Mainly contain two steps; The first step utilizes 70% ethanol or 50% propyl alcohol with the alcohol soluble protein extracting section in the flour sample, and glutelin partly is retained in the deposition after centrifugal, and it is broken to utilize manual method of milling in biuret reagent, will precipitate subsequently; And glutelin component wherein carried out chromogenic reaction, measure its content subsequently.There are two problems in this method, and the one, can only analyze the polymeric total amount of glutelin, and content that can not the big aggressiveness of separation determination glutelin; The 2nd, carry alcohol soluble protein in advance after, in biuret reagent, making mills by hand contains the deposition of glutelin, is difficult to make deposition fully broken, causes repeated very poor between parallel samples, replicate analysis makes workload significantly increase, and wastes time and energy; Make that simultaneously measured value is on the low side, depart from objective value.
Two, the n-propanol or the propyl alcohol precipitation method
This method mainly contains two kinds of application modes.
First kind of application model extracted the flour sample for directly utilizing 50% n-propanol; After centrifugal albumen in the flour is divided into two parts; Supernatant partly comprises albumin, globulin, alcohol soluble protein, and the lower glutelin condensate of the molecular weight of fraction; The deposition part comprises most glutelin condensate.The deposition part is measured protein content after dry, and is called as the content of " insoluble glutelin condensate ".Though this method is simple; But still the big aggressiveness two parts of glutelin total amount and glutelin are not separated quantification (Bean S R, Lyne R K, Tilley K A; Chung O K; Lookhart G L.A rapid method for quantitation of insoluble polymeric proteinsin flour.Cereal Chem, 1998,75:374-379).
Second kind of application model makes the n-propanol concentration of supernatant bring up to 70% at first having taked first kind of mode subsequently, makes the lower glutelin condensate of those a spot of molecular weight precipitate.The result makes flour albumen be divided into 3 parts, i.e. monomeric protein (albumin, globulin, alcohol soluble protein), glutelin condensate that the fraction molecular weight is lower, and the glutelin that is arranged in deposition of the overwhelming majority.This method is not only complicated; And the big aggressiveness two parts of glutelin total amount and glutelin are not separated quantification (Fu B X equally; Sapirstein H D.Procedure for isolating monomeric proetins andpolymeric glutenin of wheat flour.Cereal Chem; 1996,73:143-152).
Three, single subunit gel electrophoresis densitometric scan method
The glutelin condensate is the maximum natural macromolecular of existing knowledge occurring in nature molecular weight; Utilize conventional non-reduced solution and mechanical effect to be difficult to fully extract; Therefore often utilize reductive agent,, HMW in the condensate and low-molecular-weight glutenin are reduced to the monomer subunit like DDT.Separating the back through gel electrophoresis, that dyed subunit band is carried out densitometric scan is quantitative.This method (Huang D Y; KhanK.Quantitative determination of high molecular weight glutenin subunits ofhard red spring wheat by SDS-PAGE.II.Quantitative effects of individualsubunits on breadmaking quality characteristics.Cereal Chem; 1997; 74:786-790) have following several problem: what (1) was measured is single subunit or glutelin total amount, can not measure the content of the big aggressiveness of glutelin; (2) subunit of this method quantitatively will be accomplished through gel electrophoresis usually, and cost is higher, wastes time and energy and uses toxic chemical more, like acrylic amide, 4-vinylpyridine etc.
Four, the SDS phosphate buffer extracts, and multilayer concentrates glue separation+densitometric scan method
This method utilizes the SDS phosphate buffer that flour protein is divided into two parts, and first comprises the lower glutelin condensate of molecular weight of monomeric protein (albumin, globulin, alcohol soluble protein) and solubility; Second portion comprises the big aggressiveness of the higher glutelin of insoluble molecular weight.Subsequently first is carried out multilayer and concentrate glue separation, dyeing and densitometric scan; In conjunction with kjeldahl determination or near infrared determining the protein quantity to first and second portion; Calculate different molecular weight protein component in the first, and the content of the big aggressiveness of second portion glutelin (Zhu J, Khan K.Effects of genotypeand environment on glutenin polymers and bread making quality.Cereal Chem; 2001,78:125-130).The subject matter that this method exists has: (1) mensuration process and technical sophistication, and cost is higher, wastes time and energy; (2) different measuring methods is used simultaneously with repeatedly calculating and possibly caused measured value to depart from objective value.
Five, ultrasonic disruption+exclusion chromatography
This method combines the SDS phosphate buffer can measure different glutelin components contents flexibly with ultrasonic disruption.Direct ultrasonic Treatment in the SDS phosphate buffer, and can measure the polymeric total amount of glutelin through gel chromatography; Only use the concussion of SDS phosphate buffer to extract; But and through the lower polymeric content of glutelin of gel chromatography determining molecular weight; Deposition is after ultrasonic Treatment; Utilize gel chromatography can measure the content of the big aggressiveness of glutelin (Gupta R B, Khan K, MacRitchie F.Biochemical basis of flour properties in bread wheats.I.Effects of variation inthe quantity and size distribution of polymeric protein.J Cereal Sci; 1993,18:23-41).This method is simple than the method for distilling of low glutelin condensate and the big aggressiveness of glutelin to glutelin condensate total amount, molecular weight, but in quantitatively, need utilize chromatograph.Not only chromatograph equipment itself is expensive, and it is also higher to measure the consumptive material expense, and comprises that the operation of sample pre-treatments and instrument is all comparatively complicated, needs full-time quality inspection personnel, makes work efficiency reduce.
In biuret reagent,, utilize the chromogenic reaction of biuret reagent, measure the assay method of glutelin content after the colorimetric, do not appear in the newspapers at present behind the sample employing ultrasonic disruption.
Summary of the invention
The objective of the invention is to: the characteristics in view of above-mentioned various assay methods to the problem that they exist, provide a kind of wheat gluten content rapid analysis that is different from above-mentioned various assay methods in the mensuration process.
The objective of the invention is to realize like this: the quick analyzing method for glutelin content of a grow wheat, wheat sample to be measured is cleaned the representative seed of 5g～30g, utilize experiment to grind full powder; Plastics pack into from sealed bag after crossing 0.5mm aperture screen cloth, and room temperature leaves standstill the 24h equilibrium moisture at least, measures water cut; As tested sample; It is characterized in that: accurately take by weighing the 100mg tested sample and place centrifuge tube, from tested sample, extract monomeric protein separately by solvent, or after extracting the lower glutelin condensate of the molecular weight of monomeric protein, solubility simultaneously; After centrifugal, abandon supernatant, glutelin is retained in the centrifuge tube; In centrifuge tube, add the 1mL biuret reagent, after use ultrasonic disruption appearance is handled,, measure the glutelin content in the sample through the chromogenic reaction of glutelin component; Glutelin content in the described sample is meant glutelin total amount or the big aggressiveness content of glutelin in the sample.
In the present invention: the solvent that from tested sample, extracts monomeric protein separately is 70% (v/v) ethanol; The lower polymeric solvent of glutelin of molecular weight that from tested sample, extracts monomeric protein and solubility simultaneously is the SDS phosphate buffer of pH 6.90, and described monomeric protein comprises egg, globulin and alcohol soluble protein clearly.
In the present invention: the method for from tested sample, extracting monomeric protein separately is: accurately take by weighing the 100mg tested sample and place the 1.5mL centrifuge tube, add 1mL 70% (v/v) ethanol, room temperature is shaken 30min continuously, and the centrifugal 15min of 12000rpm abandons supernatant; Add identical solvent once more, shake 15min continuously, the centrifugal 15min of 12000rpm fully is dissolved in the solvent alcohol soluble protein in the tested sample.
In the present invention: after extracting monomeric protein,, centrifuge tube is inverted 5min through the centrifugal supernatant of abandoning; Glutelin is retained in the centrifuge tube; In centrifuge tube, add the 1mL biuret reagent, use the ultrasonic disruption appearance to handle 15s, shift suspending liquid to the tool lid 10mL centrifuge tube that the 4mL biuret reagent is housed with liquid-transfering gun immediately; Mixing is also put into 40 ℃ of xeothermic baking oven temperature bath 20min immediately, during put upside down respectively when 5min, 10min and 15min and rock once; Taking out the centrifugal 10min in 4000rpm, in the 560nm colorimetric, is biuret reagent with reference to background with supernatant, and the curvilinear equation with the light absorption value substitution is set up with standard protein calculates the glutelin total amount in the sample; In said curvilinear equation, correlation coefficient r >=0.9998.
In the present invention: the lower polymeric method of glutelin of molecular weight of from tested sample, extracting monomeric protein and solubility is: accurately take by weighing the 100mg tested sample and place the 1.5mL centrifuge tube; The SDS phosphate buffer that adds 1mL pH 6.90; Room temperature is shaken 20min continuously; The centrifugal 15min of 12000rpm fully is dissolved in the solvent monomeric protein and the lower glutelin condensate of molecular weight of solubility in the tested sample.
In the present invention: after extracting the lower glutelin condensate of the molecular weight of monomeric protein and solubility; Through the centrifugal supernatant of abandoning, centrifuge tube is inverted 5min, glutelin is retained in the centrifuge tube; In centrifuge tube, add the 1mL biuret reagent; Use the ultrasonic disruption appearance to handle 15s, shift suspending liquid to the tool lid 10mL centrifuge tube that the 4mL biuret reagent is housed with liquid-transfering gun immediately, mixing is also put into 40 ℃ of xeothermic baking oven temperature immediately and is bathed 20min; Taking out the centrifugal 10min in 4000rpm, in the 560nm colorimetric, is biuret reagent with reference to background with supernatant; With the curvilinear equation of light absorption value substitution with standard protein foundation; Calculate the big aggressiveness content of glutelin in the sample, in said curvilinear equation, correlation coefficient r >=0.9998.
In the present invention: the probe diameter of ultrasonic disruption appearance is Φ 3mm, and described ultrasonic disruption appearance is handled and is meant: the ultrasonic disruption appearance is handled under the output power state of 12W.
The invention has the advantages that: the ultrasonic disruption appearance has fast, the outstanding advantage of high strength and even action, can make the glutelin extraction ratio and the extraction rate of wheat higher; 70% ethanol can extract the monomeric protein that comprises alcohol soluble protein, and the SDS phosphate buffer of pH 6.90 can extract the lower glutelin condensate of molecular weight of monomeric protein and solubility simultaneously, and glutelin is retained in the centrifuge tube; Utilize the chromogenic reaction of biuret reagent, measure glutelin total amount or the content of the big aggressiveness of glutelin in the sample, not only method of operating is simple, compares with the various assay methods of present employing, has quick, cheap and clear superiority accurately.
Embodiment
Embodiment 1
In the wheat sample to be tested, choose the representative seed 20g after cleaning, use FOSSCyclotec1093 type experiment whirlwind to grind wholemeal; After crossing 0.5mm aperture screen cloth, pack wholemeal the plastics of 60mm * 90mm into from sealed bag, and stir and seal sack with spoon; It is above to the water cut uniformity that room temperature is placed 24h; According to GB 5497-85, promptly grain oil plant detection-aquametry is measured the water cut of wholemeal sample, as tested sample.
Embodiment 2
A, biuret reagent preparation:
According to measuring the sample aequum, at room temperature prepare 4%CuSO
4(w/v), 2.5% sodium potassium tartrate tetrahydrate (w/v) and 5M NaOH solution.The CuSO that in the 500mL volumetric flask, adds 30mL successively
4Solution, the potassium sodium tartrate solution of 100mL slowly adds the NaOH solution of 30mL immediately again, is settled to 500mL with distilled water at last, forms stock solution;
Before beginning to measure tested sample, get an amount of stock solution and mix, form biuret reagent with the equal-volume isopropyl alcohol.
B, the preparation of SDS phosphate buffer:
Solution 1:0.05mol/L Na
2HPO
4, 0.5%SDS (w/v);
Solution 2:0.05mol/L NaH
2PO
4, 0.5%SDS (w/v);
Get an amount of solution 1 in beaker, slowly add solution 2, continuous stirring reduces to 6.90 until mixed solution pH, is the SDS phosphate buffer solution.
Embodiment 3
Typical curve is drawn:
With protein titer (7g/dL) with the standard solution of 0.1mol/L NaOH solution dilution to 5mg/mL.Get protein titer (5mg/mL) 0.0,0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,1.0mL respectively in the 10mL test tube; Add 1.0,0.9,0.8,0.7,0.6,0.5,0.4,0.3,0.2,0.1 respectively, 0.0ml distilled water, add 4.0mL biuret reagent (a provides among the embodiment 2, down with) in each test tube; Mixing; 40 ℃ of water-bath 20min, 560nm wavelength colorimetric is measured light absorption value.With the light absorption value is horizontal ordinate, is that ordinate is made typical curve with the protein content, obtains following linear equation with one unknown:
y＝12.516x-0.0316
In the formula: x is a light absorption value, and y is the percentage composition of protein, in the present embodiment, and their correlation coefficient r=0.9999.
Embodiment 4
Sample two-story valley Tot Prot is analyzed:
Accurately take by weighing 100mg tested sample (embodiment 1 provides), place the 1.5mL centrifuge tube.Every group of 12 samples, every sample repeats for 2 times.Add 1mL 70% (v/v) ethanol.Room temperature is shaken 30min continuously, and the centrifugal 15min of 12000rpm abandons supernatant; Add 1mL 70% (v/v) ethanol again.Room temperature is shaken 15min continuously, and the centrifugal 15min of 12000rpm is inverted 5min with centrifuge tube;
In containing the 1.5mL centrifuge tube of deposition, add the 1mL biuret reagent, use ultrasonic disruption appearance VCX130PB, join Φ 3mm probe, power 12W, processing 15s extremely deposition fully suspends.
To move to through the suspending liquid of ultrasonic Treatment in the tool lid 10mL centrifuge tube that the 4mL biuret reagent is housed with liquid-transfering gun, and repeat piping and druming once, with abundant transfer sample and make suspending liquid more even.
Cover tight test tube and put into 40 ℃ of xeothermic baking oven temperature bath 20min immediately, during put upside down respectively when 5min, 10min and 15min and rock once.Take out centrifugal 10min in 4000rpm, with supernatant in the 560nm colorimetric.With reference to background is biuret reagent.
With light absorption value substitution curvilinear equation, can calculate the glutelin total amount in the sample, water cut can calculate contents on dry basis per sample.
Embodiment 5
The big aggressiveness content analysis of glutelin in the sample:
Accurately take by weighing the 100mg sample, place the 1.5mL centrifuge tube.Every group of 12 samples, every sample repeats for 2 times.Add the SDS phosphate buffer solution (b provides among the embodiment 2) of 1mL, room temperature is shaken 20min continuously, and the centrifugal 15min of 12000rpm abandons supernatant, and centrifuge tube is inverted 5min.
In containing the 1.5mL centrifuge tube of deposition, add the 1mL biuret reagent, use ultrasonic disruption appearance VCX130PB, join Φ 3mm probe, power 12W, processing 15s extremely deposition fully suspends.
To move to through the suspending liquid of ultrasonic Treatment in the tool lid 10mL centrifuge tube that the 4mL biuret reagent is housed with liquid-transfering gun, and repeat piping and druming once, with abundant transfer sample and make suspending liquid more even.
Cover tight test tube and put into 40 ℃ of xeothermic baking oven temperature bath 20min immediately, during put upside down respectively when 5min, 10min and 15min and rock once.Take out centrifugal 10min in 4000rpm, with supernatant in the 560nm colorimetric.With reference to background is biuret reagent.
With light absorption value substitution curvilinear equation, can calculate the big aggressiveness content of glutelin in the sample, water cut can calculate contents on dry basis per sample.
Embodiment 6
According to the method for embodiment 1～5, to the glutelin total amount of 85 parts of kinds (being) wheat, big aggressiveness content of glutelin and gluten strength analysis result are seen table 1 respectively for we.In table 1, the mensuration of grain protein content is used NIR method (Perten DA7200); GB 5497-85 method is used in moisture determination; Sedimentation value is pressed AACC 44-15A method; The instrument parameter of kneading dough measure to use the 10g trace appearance of kneading dough to measure (National Mfg)."-" expression data disappearance; The percentage composition of the big aggressiveness of glutelin, that is: the big aggressiveness percentage composition of glutelin (%)=(the big aggressiveness content of glutelin/glutelin total amount) * 100.
Visible by table 1, represent knead dough appearance time to peak and the ultrasonic extraction gained glutelin total amount of gluten strength, big aggressiveness content and greatly the aggressiveness percentage composition all be extremely remarkable positive correlation, related coefficient is respectively 0.60,0.77 and 0.87 (P＜0.001).And grain protein content and sedimentation value and time to peak do not have significant correlativity, show the measured glutelin component concentration of this method, and especially big aggressiveness percentage composition can well be predicted gluten quality.
Can also tell people by table 1, the glutelin total amount range of variation that grinding method and supercritical ultrasonics technology are measured is respectively 0.74%～2.47% and 0.84%～2.60%.Show relatively between genotype that the grinding method extraction ratio is 79.6%～99.4% of a ultrasonic extraction method rate.Show that this method is higher to the extraction ratio of glutelin, measured value is more accurate, more helps the evaluation of gluten quality, is beneficial to carrying out of Wheat Breeding for Quality work.
The quality trait of table 185 part kind (being) and glutelin content analysis
More than each embodiment be not to concrete restriction of the present invention.
Claims (5)
1. the quick analyzing method for glutelin content of a grow wheat is cleaned the representative seed of 5g～30g with wheat sample to be measured, utilizes experiment to grind full powder; Plastics pack into from sealed bag after crossing 0.5mm aperture screen cloth, and room temperature leaves standstill the 24h equilibrium moisture at least, measures water cut; As tested sample; Accurately take by weighing the 100mg tested sample and place centrifuge tube, from tested sample by the solvent extraction monomeric protein, or after extracting the lower glutelin condensate of the molecular weight of monomeric protein, solubility simultaneously; After centrifugal, abandon supernatant, glutelin is retained in the centrifuge tube; In centrifuge tube, add the 1mL biuret reagent, after use ultrasonic disruption appearance is handled,, measure the glutelin content in the sample through the chromogenic reaction of glutelin component; Glutelin content in the described sample is meant glutelin total amount or the big aggressiveness content of glutelin in the sample;
It is characterized in that:
To extract monomeric protein through the centrifugal supernatant of abandoning; Again centrifuge tube is inverted 5min, glutelin is retained in the centrifuge tube, in centrifuge tube, add the 1mL biuret reagent; Use the ultrasonic disruption appearance to handle 15s; Shift suspending liquid to the tool lid 10mL centrifuge tube that the 4mL biuret reagent has been housed with liquid-transfering gun immediately, mixing is also put into 40 ℃ of xeothermic baking oven temperature bath 20min immediately, during put upside down respectively when 5min, 10min and 15min and rock once; Taking out the centrifugal 10min in 4000rpm, in the 560nm colorimetric, is biuret reagent with reference to background with supernatant, and the curvilinear equation with the light absorption value substitution is set up with standard protein calculates the glutelin total amount in the sample;
The glutelin condensate that the molecular weight of the monomeric protein that extracts simultaneously, solubility is lower is through the centrifugal supernatant of abandoning; Again centrifuge tube is inverted 5min; Glutelin is retained in the centrifuge tube, in centrifuge tube, adds the 1mL biuret reagent, use the ultrasonic disruption appearance to handle 15s; Shift suspending liquid to the tool lid 10mL centrifuge tube that the 4mL biuret reagent is housed with liquid-transfering gun immediately, mixing is also put into 40 ℃ of xeothermic baking oven temperature immediately and is bathed 20min; Taking out the centrifugal 10min in 4000rpm, in the 560nm colorimetric, is biuret reagent with reference to background with supernatant, and the curvilinear equation with the light absorption value substitution is set up with standard protein calculates the big aggressiveness content of glutelin in the sample;
In said curvilinear equation, correlation coefficient r >=0.9998.
2. the quick analyzing method for glutelin content of wheat according to claim 1 is characterized in that: the solvent that from tested sample, extracts monomeric protein separately is 70% ethanol; The lower polymeric solvent of glutelin of molecular weight that from tested sample, extracts monomeric protein and solubility simultaneously is the SDS phosphate buffer of pH6.90, and described monomeric protein comprises albumin, globulin and alcohol soluble protein.
3. the quick analyzing method for glutelin content of wheat according to claim 1 and 2; It is characterized in that: the method for from tested sample, extracting monomeric protein separately is: accurately take by weighing the 100mg tested sample and place the 1.5mL centrifuge tube; Add 1mL 70% ethanol; Room temperature is shaken 30min continuously, and the centrifugal 15min of 12000rpm abandons supernatant; Add identical solvent once more, shake 15min continuously, the centrifugal 15min of 12000rpm fully is dissolved in the solvent monomeric protein in the tested sample.
4. the quick analyzing method for glutelin content of wheat according to claim 1 and 2; It is characterized in that: the lower polymeric method of glutelin of molecular weight of from tested sample, extracting monomeric protein and solubility is: accurately take by weighing the 100mg tested sample and place the 1.5mL centrifuge tube; The SDS phosphate buffer that adds 1mLpH 6.90; Room temperature is shaken 20min continuously; The centrifugal 15min of 12000rpm fully is dissolved in the solvent monomeric protein and the lower glutelin condensate of molecular weight of solubility in the tested sample.
5. the quick analyzing method for glutelin content of wheat according to claim 1; It is characterized in that: the probe diameter of ultrasonic disruption appearance is Φ 3mm, and described ultrasonic disruption appearance is handled and is meant: the ultrasonic disruption appearance is handled under the output power state of 12W.
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| CN104458959A (en) * | 2014-12-11 | 2015-03-25 | 首都师范大学 | Method for identifying glutenin macro-polymer content in wheat |
| CN104655581A (en) * | 2015-02-13 | 2015-05-27 | 河南工业大学 | Trace rapid detection method of wheat flour strength |
| CN106970041B (en) * | 2017-04-12 | 2020-04-07 | 江苏省农业科学院 | Near-infrared determination method for insoluble glutelin macro-polymer content of wheat flour |
| CN112209998B (en) * | 2020-10-10 | 2022-06-03 | 国投生物科技投资有限公司 | Ultrasonic-assisted extraction method of corn protein |
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