CN101724571A - Method for increasing yield of solid fermentation beauveria bassiana spores by adding nutrient substances - Google Patents
Method for increasing yield of solid fermentation beauveria bassiana spores by adding nutrient substances Download PDFInfo
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- CN101724571A CN101724571A CN200910264664A CN200910264664A CN101724571A CN 101724571 A CN101724571 A CN 101724571A CN 200910264664 A CN200910264664 A CN 200910264664A CN 200910264664 A CN200910264664 A CN 200910264664A CN 101724571 A CN101724571 A CN 101724571A
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- beauveria bassiana
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Abstract
The invention discloses a method for increasing the yield of solid fermentation beauveria bassiana spores by adding nutrient substances, which belongs to the technical field of application of a nutrient condition changing strategy in fermentation process optimization. The beauveria bassiana YP3-09 of the invention is preserved in CCTCC, and the preservation number is CCTCC No: M209238. The CCTCC No: M209238 is used as a fermentation strain; 33 to 37 g/L of sucrose, 50 to 55 g/L of sodium nitrate and 75 to 85 mug/L of boric acid are added into a solid fermentation culture medium to perform fermentation; and under the culture conditions, the yield of the beauveria bassiana spores is increased to 6.22*109 spores per gram from 7.30*107 spores per gram of a contrast (without adding three nutrient elements) case; therefore, the yield of the beauveria bassiana spores can be greatly increased by using the strategy, and the method provides the foundation for industrialized production. The strategy has important instructive significance for the production of other solid fermentation products.
Description
Technical field
A kind ofly improve the method for solid state fermentation beauveria bassiana spore output, relate to nutritive substance and be added on the applied technical field of solid ferment process in optimizing with adding nutritive substance.
Background technology
Muscardine (Beauveria bassiana) is a kind of insect pathogenic fungus of broad spectrum, has significant application potential on the control agriculture and forestry injurious insect.Beauveria bassiana spore can be by liquid state fermentation, semi-solid ferment, solid fermentation production.Because the solid state fermentation cost is low, the product toxicity height, solid state fermentation becomes prefered method in beauveria bassiana spore production.Research to solid state fermentation production beauveria bassiana spore at present mainly concentrates on Optimizing Conditions of Fermentation, as Optimum of culture medium, and DESIGN OF REACTOR optimization.The output of the strategy raising beauveria bassiana spore of nutritive substance is added in utilization in solid-state fermentation culture medium, do not see bibliographical information as yet.
Summary of the invention
The purpose of this invention is to provide a kind of method that the strategy that adds nutritive substance in the solid-state fermentation culture medium improves fermentative production beauveria bassiana spore output that is used in, utilize the present invention can improve the output of beauveria bassiana spore in a large number.
Technical scheme of the present invention: the bacterial strain of beauveria bassiana spore is produced in strain fermentation, and its classification called after beauveria bassiana (Beauveria bassiana) YP3-09 has been preserved in Chinese typical culture collection center, and deposit number is CCTCC NO:M 209238.
A kind of with the method for adding nutritive substance raising solid state fermentation beauveria bassiana spore output, with CCTCC NO:M 209238 is the zymophyte pearl, in solid-state fermentation culture medium, add the sucrose of nutritive substance: 33-37g/L, the SODIUMNITRATE of 50-55g/L and the boric acid of 75-85 μ g/L and ferment, improve beauveria bassiana spore output.
Add nutritive substance in solid medium: the sucrose, the SODIUMNITRATE of 52.8g/L and the boric acid of 79.6 μ g/L that add 34.7g/L ferment, and improve the output of beauveria bassiana spore.
Beneficial effect of the present invention: utilize the present invention can improve the output of beauveria bassiana spore in a large number.Spore output is by 7.30 * 10
7Individual/g brings up to 6.22 * 10
9Individual/g.Beauveria bassiana spore can be used for suppressing the tealeaves leafhopper, and a large amount of raisings of its spore output have promoted the process of industrialization that beauveria bassiana spore is produced.The strategy that adds nutritive substance in solid medium of the present invention has certain directive significance to the production of other solid fermentation products.
The biological material specimens preservation
Beauveria bassiana (Beauveria bassiana) YP3-09 has been preserved in Chinese typical culture collection center, is called for short CCTCC, and preservation date is on October 23rd, 2009, and deposit number is CCTCC NO:M 209238.
Embodiment
Comparative examples: the used bacterial classification of the present invention is beauveria bassiana (Beauveria bassiana) YP3-09, be CCTCC NO:M 209238, the spore of scraping earlier on the inclined-plane is cultivated 42h in shaking bottle, 25 ℃ of temperature, rotating speed 180rpm, then seed liquor is received solid medium top fermentation 7d, fermentation condition is: 25 ℃ of temperature, humidity 95%.Solid-state fermentation culture medium is: wheat bran: 872.5g, rice husk: 40g, H
2O:1000mL.Beauveria bassiana spore output is 7.30 * 10
7Individual/g.
Embodiment 1: solid state fermentation adopts substratum to add the nutritive substance strategy, adds sucrose, the SODIUMNITRATE of 52.8g/L and the boric acid of 79.6 μ g/L of 34.7g/L in the solid-state fermentation culture medium, the same comparative examples of all the other conditions.The result: beauveria bassiana spore output is: 6.22 * 10
9Individual/g.
Claims (3)
1. the bacterial strain of beauveria bassiana spore is produced in strain fermentation, and its classification called after beauveria bassiana (Beauveriabassiana) YP3-09 has been preserved in Chinese typical culture collection center, and deposit number is CCTCC NO:M 209238.
2. one kind is improved the method for solid state fermentation beauveria bassiana spore output with adding nutritive substance, it is characterized in that with CCTCC NO:M 209238 be the zymophyte pearl, in solid-state fermentation culture medium, add the sucrose of nutritive substance: 33-37g/L, the SODIUMNITRATE of 50-55g/L and the boric acid of 75-85ug/L and ferment, improve beauveria bassiana spore output.
3. method according to claim 2 is characterized in that adding the sucrose of nutritive substance: 34.7g/L, the SODIUMNITRATE of 52.8g/L and the boric acid of 79.6ug/L and ferments in solid-state fermentation culture medium, improve the output of beauveria bassiana spore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102646648A CN101724571B (en) | 2009-12-21 | 2009-12-21 | Method for increasing yield of solid fermentation beauveria bassiana spores by adding nutrient substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102646648A CN101724571B (en) | 2009-12-21 | 2009-12-21 | Method for increasing yield of solid fermentation beauveria bassiana spores by adding nutrient substances |
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| Publication Number | Publication Date |
|---|---|
| CN101724571A true CN101724571A (en) | 2010-06-09 |
| CN101724571B CN101724571B (en) | 2011-07-27 |
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| CN2009102646648A Expired - Fee Related CN101724571B (en) | 2009-12-21 | 2009-12-21 | Method for increasing yield of solid fermentation beauveria bassiana spores by adding nutrient substances |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321543A (en) * | 2011-08-30 | 2012-01-18 | 江南大学 | Carbohydrate protective agent for increasing heat resistance of beauveria bassiana spore |
| CN102925361A (en) * | 2012-08-10 | 2013-02-13 | 河南普爱饲料股份有限公司 | AFB1 degrading bacterium and degrading enzyme |
| CN103881930A (en) * | 2014-03-28 | 2014-06-25 | 东北农业大学 | Solid culture medium and culture method of beauveria |
| CN105767015A (en) * | 2016-05-25 | 2016-07-20 | 句容市天王镇戴庄有机农业专业合作社 | Method for preventing and treating rice planthoppers through beauveria bassiana |
| CN107916227A (en) * | 2016-10-10 | 2018-04-17 | 浙江泛亚生物医药股份有限公司 | A kind of suppression of Strain of Beauveria bassiana to tyrosinase activity |
-
2009
- 2009-12-21 CN CN2009102646648A patent/CN101724571B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321543A (en) * | 2011-08-30 | 2012-01-18 | 江南大学 | Carbohydrate protective agent for increasing heat resistance of beauveria bassiana spore |
| CN102925361A (en) * | 2012-08-10 | 2013-02-13 | 河南普爱饲料股份有限公司 | AFB1 degrading bacterium and degrading enzyme |
| CN102925361B (en) * | 2012-08-10 | 2014-12-24 | 河南普爱饲料股份有限公司 | AFB1 degrading bacterium and degrading enzyme |
| CN103881930A (en) * | 2014-03-28 | 2014-06-25 | 东北农业大学 | Solid culture medium and culture method of beauveria |
| CN105767015A (en) * | 2016-05-25 | 2016-07-20 | 句容市天王镇戴庄有机农业专业合作社 | Method for preventing and treating rice planthoppers through beauveria bassiana |
| CN107916227A (en) * | 2016-10-10 | 2018-04-17 | 浙江泛亚生物医药股份有限公司 | A kind of suppression of Strain of Beauveria bassiana to tyrosinase activity |
| CN107916227B (en) * | 2016-10-10 | 2021-02-09 | 浙江泛亚保健食品有限公司 | Inhibition of beauveria bassiana strain on tyrosinase activity |
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| Publication number | Publication date |
|---|---|
| CN101724571B (en) | 2011-07-27 |
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Granted publication date: 20110727 Termination date: 20121221 |