CN101718704A - Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof - Google Patents
Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof Download PDFInfo
- Publication number
- CN101718704A CN101718704A CN200910194192A CN200910194192A CN101718704A CN 101718704 A CN101718704 A CN 101718704A CN 200910194192 A CN200910194192 A CN 200910194192A CN 200910194192 A CN200910194192 A CN 200910194192A CN 101718704 A CN101718704 A CN 101718704A
- Authority
- CN
- China
- Prior art keywords
- test paper
- solution
- detection
- phenolphthalein
- phenothalin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for preparing a test paper for quickly detecting phenothalin, a detection kit and a detection method. The test paper is prepared by soaking neutral filter paper in alkaline solution at a pH value of 11 to 12, taking the filter paper out and drying the filter paper. The alkaline solution may be solution of NaOH, Na2CO3 or KOH. The test paper prepared by the method is assembled with ethanol solution to form the detection kit. The method for detecting phenothalin by using the test paper for detection comprises: adding a test product in the ethanol solution, shaking the ethanol solution and standing the ethanol solution; soaking the test paper for detection in the processed solution of the test product; and taking the test paper for detection out of the solution, wherein if the test paper shows a rose color, that the test product contains phenothalin can be determined, and if the test paper does not show a rose color, that the test product does not contain phenothalin can be determined. The test paper, the related kit and the method for quickly detecting phenothalin can quickly and accurately detect phenothalin, particularly can detect whether phenothalin is illegally added in weight-reducing Chinese patent medicaments, health-care foods and other foods in situ, are simple and convenient in operation, suitable for in-situ detection and can realize semi-quantitative detection.
Description
Technical field
The present invention relates to the detection technique of phenolphthalein, specifically relate to quick detection test paper and the detection kit and the detection method of phenolphthalein.
Background technology
Along with growth in the living standard, it is healthy that people recognize that obesity has a strong impact on, and also recognizes with the Western medicine quick weight-lossing simultaneously and can bring serious adverse.But the fat-reducing effect of Chinese medicine class slimming drugs needs the long period just can embody, and is difficult to cause people's desire to purchase.Therefore, in the face of huge market and profit, the understanding mistaken ideas that some illegal manufacturers utilize psychology that the consumer is eager to lose weight and Chinese medicine to have no side effect, the chemical slimming drugs that a large amount of interpolation state's laws rules are prohibited in fat-reducing class Chinese patent drug, health food and food, cathartic are to strengthen its fat-reducing effect, cause the consumer under unwitting situation, take this series products continuously, serious harm consumers in general's health.
Common chemical slimming drugs and cathartic have sibutramine hydrochloride, fenfluramine hydrochloride, magnesium sulphate, phenolphthalein etc.Fenfluramine is mainly used in treatment simple obesity and with diabetes, coronary heart disease and hypertensive obesity clinically.Studies show that, use fenfluramine can cause serious side effects such as damaged heart valve, pulmonary hypertension and finger necrosis, 1997, this medicine of FDA decision forbidding, 13 kinds of slimming drugs that comprise fenfluramine/Dexfenfluramine are cancelled in decision in the Official Journal in European Union on April 4th, 2000.Sibutramine is a kind of non-amphetamine anorectic, pharmacological action is similar to fenfluramine, China allows to use the sibutramine fat-reducing, but need carefully use this type of medicine, should be strict during use according to indication, dosage must not surpass 15mg and stipulate and should use in accordance with the package insert regulation under physician guidance every day, and the health in the use must be monitored just at any time can guarantee medication.Magnesium sulphate uses as cathartic and had century-old history, and the magnesium sulphate of overdose causes severe impairment can for stomach, enteron aisle, bladder and the kidney of human body.After finding that phenolphthalein can be used as the cathartic use and has the effect that is better than magnesium sulphate, the status that magnesium sulphate is used as cathartic is replaced by phenolphthalein gradually.But, phenolphthalein easily causes the human allergy, causes symptoms such as dermatitis, drug rash, itch and cusalgia, and prolonged application can make blood sugar increasing, blood potassium reduce, excessive or long-term abuse can cause electrolyte disturbance, bring out arrhythmia cordis, also symptoms such as obnubilation, muscle spasmus and lassitude can take place.
After 2007, increase gradually about the research of phenolphthalein detection method and report, but mainly concentrate on the detection method of utilizing high-end analytical instrument to carry out.Though methods such as high performance liquid chromatography, thin-layered chromatography and liquid chromatograph mass spectrography can be carried out the phenolphthalein in the Chinese medicine accurate qualitative and quantitative, the sense cycle of these methods is long, process is loaded down with trivial details, is not suitable for on-the-spot examination in batches.In addition, chromatogram check and analysis instrument costs an arm and a leg, volume is bigger, and the check and analysis cost is higher, requires operating personnel to have higher level professional technology, and the medicine prison personnel of basic unit are difficult to grasp its operation, are not suitable for the examination of medicine in enormous quantities.
Summary of the invention
For overcoming above-mentioned technological deficiency, the invention provides the test paper that a kind of phenolphthalein fast detecting is used, and related kit and detection method, detect existing defective to overcome the high-end analytical instrument of above-mentioned usefulness.
For achieving the above object, the present invention at first provides a kind of preparation method of quick detection test paper of phenolphthalein, and concrete operations are as follows: get neutral filter paper, placing pH value is the alkaline solution of 11-12, soaks, and takes out drying.The time of soaking can be between 0.5 to 1 minute.Drying mode can adopt dry, air-dry or the oven dry.
Preferably, described alkaline solution comprises NaOH solution, Na
2CO
3Solution or KOH solution.Wherein, NaOH solution, Na
2CO
3The quality concentration of volume percent of the solute in solution or the KOH solution is 3%～10%.
Utilize above-mentioned detection test paper and other solution to be assembled into a kind of quick detection kit of phenolphthalein, this kit also comprises ethanolic solution except detecting test paper.
Preferably, the concentration of volume percent of the ethanol in the ethanolic solution is 90～96%.
The present invention also provides the method for utilizing above-mentioned detection detection paper phenolphthalein, gets test sample and adds in the ethanolic solution, and jolting is left standstill; Described detection test paper is immersed in the need testing solution after handling, takes out, if test paper be rose-red then judge contain phenolphthalein, otherwise, then be judged as and do not contain phenolphthalein.When test sample is liquid, can directly add ethanolic solution; When test sample is solid, described test sample is ground or crushing.
Above-mentioned detection test paper can be applicable in the detection at phenolphthalein in fat-reducing class Chinese patent drug, health food and the food.
Phenolphthalein quick detection test paper of the present invention and related kit and method thereof, can detect phenolphthalein rapidly and accurately, whether particularly can identify in fat-reducing class Chinese patent drug, health food and the food illegal interpolation phenolphthalein at the scene, the operation simple and convenient, be fit to on-the-spot the detection, can also realize half-quantitative detection; The behavior that helps improving supervision and inspection efficient and hit illegal interpolation phenolphthalein for the security control of Chinese patent drug, health food and food provides effective detection means, to regulating market order, protects consumers in general's rights and interests to have great importance conscientiously.
Description of drawings
Fig. 1 detects No. 41 samples, No. 62-green capsule sample and No. 48 test result of samples;
Fig. 2 is the test findings figure of phenolphthalein minimum detectability in No. 41 samples;
Fig. 3 is the test findings figure of phenolphthalein minimum detectability in the reference substance.
Embodiment
For making the present invention easier to understand,, further set forth the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in to limit the scope of the invention that NM concrete experimental technique in the following example carries out according to the normal experiment method usually.
Embodiment 1
The preparation method of the detection test paper of present embodiment is as follows: get neutral filter paper, placing pH value is that 11 NaOH solution soaked 0.5 minute, takes out, and dries.Wherein, the quality concentration of volume percent of NaOH is 3%.
Embodiment 2
The preparation method of the detection test paper of present embodiment is as follows: get neutral filter paper, placing pH value is 12 Na
2CO
3Soaked 1 minute in the solution, take out, air-dry.Wherein, Na
2CO
3The quality concentration of volume percent be 10%.
Embodiment 3
The preparation method of the detection test paper of present embodiment is as follows: get neutral filter paper, placing pH value is that 12 KOH solution soaked 0.7 minute, takes out oven dry.Wherein, the quality concentration of volume percent of KOH is 8%.
Embodiment 4
The quick detection kit of the phenolphthalein of present embodiment comprises the detection test paper, also comprises ethanolic solution.The concentration of volume percent of the ethanol in the ethanolic solution is 90%.
In another embodiment, the concentration of volume percent of the ethanol in the ethanolic solution is 96%.
In another embodiment, the concentration of volume percent of the ethanol in the ethanolic solution is 92%.
Embodiment 5: Preliminary detection
Present embodiment provides the kit among a kind of embodiment of utilization 4 to carry out the method for phenolphthalein in fast detecting fat-reducing class Chinese patent drug, health food and the food.This method is to get each 1 of the capsule of No. 41 samples, No. 62-green capsule, No. 48 samples, is ground into powder, and this powder is added in ethanolic solution again, and ethanolic solution can be 3-5ml, and jolting 1 minute is left standstill, and as seen has sediment to occur; To detect at last in the supernatant that test paper immerses the need testing solution after the Ethanol Treatment, take out after half second.If test paper be rose-red then judge contain phenolphthalein in the test sample; Otherwise, then judge not contain phenolphthalein in the test sample.
Wherein, No. 41 samples: the fruit tea thin capsule of pumping, authentication code: the strong word G20080301 of state's food, manufacturer: the Guangzhou wheat is obtained the health bio tech ltd, Yunnan Guanyuan living resources development corporation, Ltd., lot number: 090601/3; No. 62-green capsule: the green capsule in the green graceful slimming capsule, authentication code: the strong word G20050386 of state's food, manufacturer: Zun Yi health supreme being bio tech ltd, lot number: 200904091; No. 48 samples: balsam pear fat eliminating weight reducing capsule (" the thin waist of U.S. leg " type), authentication code: defend No. the 0462nd, the accurate word (2004) of food, manufacturer: the grand medicine bioengineering in Qinghai Science and Technology Ltd., lot number: 2009.01.06.
Testing result is seen Fig. 1, and among Fig. 1, the left side is the result of No. 41 sample detection, and the centre is the testing result of No. 62-green capsule, and the right side is No. 48 test result of samples.As seen from the figure, the colour developing result of No. 41 samples and No. 62-green capsule is rose-red, shows in these two kinds of samples to contain phenolphthalein.The colour developing result of No. 48 samples does not show rose-red, judges that tentatively it does not contain phenolphthalein.
In another embodiment, when detecting tablet, tea in bag, granule, oral liquid, tablet is got 1 detection consumption, and tea in bag, granule are got the detection consumption of about 1/2 bag, and liquid preparations such as oral liquid are got dose 1/2 time; Tablet and granule need to grind in advance or crushing.Other steps are the same.
Embodiment 6: phenolphthalein minimum detectability test in the sample
Get and be numbered No. 41 sample contents, according to the HPLC-DAD measurement result, this sample phenolphthalein content is 49.3mg/g.Accurate claim fixed this sample 0.4260g, put in the 50ml measuring bottle, add ethanol 30ml, ultrasonic Extraction 5 minutes is put coldly, adds the scale of ethanol dilution to 50ml, shakes up, and filters.Draw the different amounts of this solution respectively and make per 1 milliliter of sample gradient solution that contains phenolphthalein 0.021mg, 0.042mg, 0.084mg, 0.126mg, 0.168mg, 0.210mg, 0.42mg.Get each 10 μ l of this sample gradient solution respectively, put from left to right in detecting on the test paper.Observe change color, and the record experimental result.Phenolphthalein minimum detectability test findings sees Table 1 and Fig. 2 in the sample.
Table 1
| Numbering | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 | ??7 |
| Point sample amount (μ l) | ??10 | ??10 | ??10 | ??10 | ??10 | ??10 | ??10 |
| Phenolphthalein content (μ g) | ??4.20 | ??2.10 | ??1.68 | ??1.26 | ??0.84 | ??0.42 | ??0.21 |
| Change color | ??++++ | ??++++ | ??++++ | ??++++ | ??+++ | ??++ | ??+ |
Annotate: "+" expression change color degree, 2 "+" above expression naked eyes can be distinguished, the no change color of "-" expression.
By table 1 and Fig. 2 as seen, the colour developing degree of depth of reaction is along with the height of concentration presents regular the variation, and the concentration of phenolphthalein is high more in the sample, and then Fan Ying colour developing is dark more, and concentration is low more, and then Fan Ying colour developing is shallow more.Test paper of the present invention can carry out half-quantitative detection to phenolphthalein in the sample in view of the above.When the concentration of phenolphthalein in the sample was reduced to 0.021 μ g/ μ l, whether naked eyes almost can not be distinguished had color to present.Therefore, the phenolphthalein concentration limit of inferring this test paper is about 0.02 μ g/ μ l.
Simultaneously, also carried out the experiment of minimum detectability with the phenolphthalein reference substance.At first precision takes by weighing phenolphthalein reference substance 10.25mg and puts in the 25ml measuring bottle, adds ethanol and makes dissolving and be diluted to scale, shakes up.Draw the different amounts of this reference substance solution respectively and make per 1 milliliter of reference substance gradient solution that contains phenolphthalein 0.02～0.41mg.Get each 10 μ l of reference substance gradient solution respectively, put from left to right in detecting on the test paper.Observe change color, and the record experimental result, this results are shown in Table 2 and Fig. 3.
Table 2
| Numbering | ??1 | ??2 | ??3 | ??4 | ??5 | ??6 | ??7 |
| Point sample amount (μ l) | ??10 | ??10 | ??10 | ??10 | ??10 | ??10 | ??10 |
| Phenolphthalein content (μ g) | ??4.10 | ??2.05 | ??1.64 | ??1.23 | ??0.82 | ??0.41 | ??0.20 |
| Change color | ??++++ | ??++++ | ??++++ | ??++++ | ??+++ | ??++ | ??+ |
Annotate: "+" expression change color degree, 2 "+" above expression naked eyes can be distinguished, the no change color of "-" expression.
By table 2 and Fig. 3 as seen, the colour developing degree of depth of the reaction of phenolphthalein reference substance is along with the height of concentration presents regular the variation, and the concentration of phenolphthalein is high more in the reference substance, and then Fan Ying colour developing is dark more, and concentration is low more, and then Fan Ying colour developing is shallow more.And when the concentration of phenolphthalein in the sample was reduced to 0.02 μ g/ μ l, whether naked eyes almost can not be distinguished had color to present.Coincide with the result of phenolphthalein in the aforementioned test sample.
Because the adsorbance of test strips is advisable with 20 μ l, thereby this method is 0.4 μ g to the minimum detectability of phenolphthalein, has higher sensitivity.For the onset dosage (50mg) of phenolphthalein, this method is applicable to the rapid screening of the phenolphthalein that whether mixes in fat-reducing class Chinese patent drug, health food and the food.
Embodiment 7: the detection kit with embodiment 4 detects doping phenolphthalein and compares demonstration test with thin-layered chromatography and high performance liquid chromatograph-diode array detector (HPLC-DAD) detection method in conjunction with method of the present invention
With the inventive method 62 kinds of samples are carried out fast detecting, and compare checking with conventional thin-layered chromatography (TLC) and high performance liquid chromatograph-diode array detector (HPLC-DAD) detection method.
Thin-layered chromatography is as preliminary conclusive evidence method, and concrete experimental implementation is as follows:
Test sample is handled and is carried out according to the method among the present invention.It is an amount of that other gets the phenolphthalein reference substance, adds ethanol and make every 1ml and contain the solution that phenolphthalein is 0.5mg, product solution in contrast.Test according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 5 μ l of need testing solution and reference substance solution respectively, putting respectively on same silica GF254 thin layer plate, is developping agent with methenyl choloride-methyl alcohol (19: 1), launches, dry, put under the ultraviolet lamp (254nm) and inspect, spray again, put under the daylight and inspect with 2% sodium hydroxide solution, if the test sample chromatogram is identical with the position with a reference substance chromatogram apparent spot colors, then decidable is may contain phenolphthalein in the test sample.
High performance liquid chromatograph-diode array detector (HPLC-DAD) is as confirmation method, and concrete experimental implementation is as follows:
Chromatographic condition and system suitability test: with the octadecylsilane chemically bonded silica is packed column, is moving phase with acetonitrile-water (50: 50); The detection wavelength is 275nm; Column temperature is 30 ℃.Number of theoretical plate calculates by the phenolphthalein peak should be not less than 1500.
The preparation of reference substance solution: it is an amount of to get the phenolphthalein reference substance, accurate claims surely, adds absolute ethyl alcohol and makes every 1ml and contain the solution that phenolphthalein is 0.12mg, promptly;
The preparation of need testing solution: if test sample is a tablet, porphyrize, precision claims to conclude a contract or treaty 1 amount; If test sample is a capsule, take out the content porphyrize, precision claims to conclude a contract or treaty 1 amount; If solid pharmaceutical preparation such as granule, tea in bag grinds, the accurate 1/2 bag amount of concludeing a contract or treaty that claims; If test sample is an oral liquid, measure dose 1/2 time; Put respectively in the 25ml measuring bottle, it is an amount of to add absolute ethyl alcohol, and sonicated 10 minutes is put and is chilled to room temperature, is diluted to scale with absolute ethyl alcohol, shakes up, and filters, and gets subsequent filtrate, promptly.
Determination method: accurate respectively the absorption according to product solution and each 10 μ l of need testing solution, inject liquid chromatograph, measure, promptly.
The chromatographic peak consistent with the reference substance retention time must not appear in the test sample chromatogram.As the chromatographic peak consistent occurring with the reference substance retention time, then should adopt diode array detector to detect, if this chromatographic peak is identical with the ultra-violet absorption spectrum of reference substance, then can judges and contain phenolphthalein.
By table 3 result as seen, testing result of the present invention and back two kinds of methods have the higher goodness of fit, and concrete data see Table 3.
Table 3
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??1 | More tender beautiful slim tea | Defend No. the 052nd, the strong word (1997) of feed, the healthy product (Shantou) of peace company limited | ??080702 | Negative | ??/ | Negative |
| ??2 | Big impression slim tea | No. the 104th, hygienic food and health number (1997), the big impression health products in Guangdong company limited | ??N080701 | Negative | ??/ | Negative |
| ??3 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??04080531-02019 | Negative | ??/ | Negative |
| ??4 | The Kang Liyuan tea in bag of losing weight | No. the 0082nd, hygienic food and health number [2003], the Beijing Out Shure Health Care Products Co., Ltd | ??05080301-0001 | Negative | ??/ | Negative |
| ??5 | Green graceful slimming capsule | The strong word G20050386 of state's food, Zun Yi health supreme being bio tech ltd | ??200805191 | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??6 | The gold thread slimming capsule | No. the 002nd, No. 0308 strong new word (2000) of E Wei food of hygienic food and health number (1990), Wuhan gold thread bioengineering company limited | ??080416 | The black capsules feminine gender, white capsule feminine gender | ??/ | The black capsules feminine gender, white capsule feminine gender |
| ??7 | Bent beautiful Man Linna sea life slimming capsule (combination of weight reducing skin-active economic benefits and social benefits) | The strong word G20030019 of state food, U.S. bioengineering company limited is created in Hainan | ??080403 | The green capsule feminine gender, the red capsule positive | The green capsule feminine gender, the red capsule positive | The green capsule feminine gender, the red capsule positive |
| ??8 | The auspicious moral dream of green source of students tea in bag | The strong word G20060633 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??020802026057 | Negative | ??/ | Negative |
| ??9 | The acceleration slimming capsule | No. the 0265th, hygienic food and health number (2003), Guizhou Kang Ling health products company limited | ??20080527 | Positive | Positive | Positive |
| ??10 | Drive and give birth to the hall slim tea | No. the 0055th, hygienic food and health number (2003), Beijing Yu Shengtang bioengineering company limited | ??080401305 | Negative | ??/ | Negative |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??11 | Kang Er longevity slim tea | No. the 020th, hygienic food and health number [1997], Sichuan Kang Er longevity health products company limited | ??20070622 | Negative | ??/ | Negative |
| ??12 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??04080333-5413 | Negative | ??/ | Negative |
| ??13 | U.S.'s Curacao gold aloe toxin expelling weight reducing beauty tea (Nan'ao) | (hygiene permits) defends food word [2006] A1-004 numbers forever, Yongfu, Guilin benefit green tea factory | ??20070402 | Negative | ??/ | Negative |
| ??14 | The aloe slim tea | No. the 0440th, hygienic food and health number (1999), the fragrant grand health products in Anxi, Fujian company limited | ??080606 | Negative | ??/ | Negative |
| ??15 | Combustion fat beauty slimming capsule | The strong word G20050239 of state's food, the careless bio tech ltd of Kunming spirit | ??080407 | Negative | ??/ | Negative |
| ??16 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??04080319-6099 | Negative | ??/ | Negative |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??17 | Green graceful slimming capsule | The strong word G20050386 of state's food, Zun Yi health supreme being bio tech ltd | ??200805191 | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender |
| ??18 | Very pretty fat-reducing and beauty nourishing capsule | No. the 0803rd, hygienic food and health number (2002), the Guangxi living health products of benefit company limited | ??080408 | The black capsules feminine gender, white capsule feminine gender | ??/ | The black capsules feminine gender, white capsule feminine gender |
| ??19 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??04080423-6011 | Negative | ??/ | Negative |
| ??20 | The aloe slim tea | No. the 0440th, hygienic food and health number (1999), the fragrant grand health products in Anxi, Fujian company limited | ??080507 | Negative | ??/ | Negative |
| ??21 | Thin edge is moulded soft capsule gently | No. the 0649th, hygienic food and health number (2002), Heilungkiang hundred safe pharmaceutcal corporation, Ltds | ??20070423 | Positive | Positive | Positive |
| ??22 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??040805156059 | Negative | ??/ | Negative |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??23 | The aloe slim tea | No. the 0440th, hygienic food and health number (1999), Guangzhou authority's health products company limited | ??080606 | Negative | ??/ | Negative |
| ??24 | The auspicious moral dream of green source of students tea in bag | The strong word G20060633 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??020712026050 | Negative | ??/ | Negative |
| ??25 | Tripe sirloin sky phoenix lipopenicillinase glue disappears | Hygienic food and health number (1998) | ??080601 | Negative | ??/ | Negative |
| Capsule | No. 017, the fragrant bio tech ltd of Guangzhou swallow | |||||
| ??26 | Green source of students slim tea | The strong word G20040371 of state's food, the Beijing Out Shure Health Care Products Co., Ltd | ??040803316011 | Negative | ??/ | Negative |
| ??27 | The gold thread slimming capsule | No. the 0308th, hygienic food and health number (1999), Wuhan gold thread bioengineering company limited | ??080102 | The black capsules feminine gender, white capsule feminine gender | ??/ | The black capsules feminine gender, white capsule feminine gender |
| ??28 | Green graceful slimming capsule | The strong word G20050386 of state's food, Zun Yi health supreme being bio tech ltd | ??200805192 | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??29 | Combustion fat beauty slimming capsule | The strong word G20050239 of state's food, the careless bio tech ltd of Kunming spirit | ??080407 | Negative | ??/ | Negative |
| ??30 | Thin beauty Holy Ghost board spirulina slimming capsule | No. the 0347th, hygienic food and health number (2000), sky, Shaanxi dragon bio tech ltd | ??2008050383 | Positive | Positive | Positive |
| ??31 | Superfine product weight reducing Tian Tianxue thins the ease constipation capsule | No. the 0459th, hygienic food and health number (2003), Fujian Province's happiness bio tech ltd | ??2008050629 | Positive | Positive | Positive |
| ??32 | Green graceful slimming capsule | The strong word G20050386 of state's food, international corporation of the new special medical group of Hong Kong health supreme being | ??200805191 | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender | The green capsule positive, the red capsule feminine gender |
| ??33 | The gold thread slimming capsule | No. the 0308th, hygienic food and health number (1999), Wuhan gold thread bioengineering company limited | ??080102 | The black capsules feminine gender, white capsule feminine gender | ??/ | The black capsules feminine gender, white capsule feminine gender |
| ??34 | Green source of students slimming capsule | The strong word G20040371 of state's food, Beijing Ao Teshuer health products development corporation, Ltd. | ??040803456026 | Negative | ??/ | Negative |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??35 | The beautiful capsule of the natural weight reducing ten degree boards of wonderful red hall | The strong word G20060386 of state's food, wonderful red the health products company limited in Guangzhou | ??20080601 | Positive | Positive | Positive |
| ??36 | The auspicious moral dream of green source of students tea in bag | The strong word G20060633 of state's food, Beijing Ao Teshuer health products development corporation, Ltd. | ??02070503-0007 | Negative | ??/ | Negative |
| ??37 | Very fast weight reducing spirulina slimming capsule (Sha Qi) | No. the 0347th, hygienic food and health number [2000], sky, Shaanxi dragon biotechnology Ltd | ??20080121 | Negative | ??/ | Negative |
| ??38 | Dexfenfluramine Hudrochlorid | The accurate word 20043336 of traditional Chinese medicines, Shaanxi Hualong Pharmaceutical Co., Ltd. | ??20090401 | Negative | ??/ | Negative |
| ??39 | Dexfenfluramine Hudrochlorid | The accurate word 20043336 of traditional Chinese medicines, Shaanxi Hualong Pharmaceutical Co., Ltd. | ??20080501 | Negative | ??/ | Negative |
| ??40 | Wonderful slimming capsule | No. the 0133rd, hygienic food and health number (2003), Shenzhen Benign Medicine Shop Industrial Co., Ltd. | ??200702 | Negative | ??/ | Negative |
| Numbering | The sample title | Manufacturer's (authentication code) | Lot number | ??TLC | ??HPLC-DAD | Test paper is judged |
| ??41 | The fruit tea thin capsule of pumping | The strong word G20080301 of state's food, Guangzhou wheat reed health bio tech ltd, Yunnan Guanyuan living resources development corporation, Ltd. | ??090601/3 | Positive | Positive | Positive |
| ??42 | Balsam pear show body cellulose capsule | Green grass or young crops is defended the prison food and comments word (2007) No. 1001, the Qinghai healthy development in science and technology of west army company limited | ??20090501 | Negative | ??/ | Negative |
| ??43 | The fruit tea thin capsule (present: fat-reducing the factor capsule) of pumping | No. the 0242nd, food card word (2006), Henan Bang Ruite pharmaceutcal corporation, Ltd are defended in Henan | ??20080213010 | Negative | Negative | Negative |
| ??44 | LUHUI PAIDU YANGYAN JIAONANG | The strong word G20040942 of state food, the Fu Ningchun of the Xiamen City food that keeps healthy | ??20090401 | Positive | Positive | Positive |
Last institute should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention but not limiting the scope of the invention; although the present invention has been done detailed description with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the essence and the scope of technical solution of the present invention.
Claims (10)
1. the preparation method of the quick detection test paper of a phenolphthalein is characterized in that, gets neutral filter paper, and placing pH value is the alkaline solution of 11-12, soaks, and takes out drying.
2. the preparation method of detection test paper according to claim 1 is characterized in that, described alkaline solution comprises NaOH solution, Na
2CO
3Solution or KOH solution.
3. the preparation method of detection test paper according to claim 2 is characterized in that, described NaOH solution, Na
2CO
3The quality concentration of volume percent of the solute in solution or the KOH solution is 3%～10%.
4. the preparation method of detection test paper according to claim 1 is characterized in that, the time of described immersion is 0.5-1 minute.
5. the preparation method of detection test paper according to claim 1 is characterized in that, the mode of described drying for dry, air-dry or the oven dry.
6. the detection test paper that obtains of the described preparation method of one of claim 1-5.
7. a quick detection kit that comprises the phenolphthalein of the described detection test paper of claim 6 is characterized in that described kit also comprises ethanolic solution.
8. detection kit according to claim 7 is characterized in that, the concentration of volume percent of the ethanol in the described ethanolic solution is 90～96%.
9. utilize the method for the described detection detection paper of claim 6 phenolphthalein, it is characterized in that, get test sample and add in the ethanolic solution, jolting is left standstill; Described detection test paper is immersed in the need testing solution after handling, takes out, if test paper be rose-red then judge contain phenolphthalein, otherwise, then be judged as and do not contain phenolphthalein.
10. the application of the described detection test paper of claim 6 phenolphthalein in detecting fat-reducing class Chinese patent drug, health food and food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910194192A CN101718704A (en) | 2009-11-26 | 2009-11-26 | Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910194192A CN101718704A (en) | 2009-11-26 | 2009-11-26 | Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101718704A true CN101718704A (en) | 2010-06-02 |
Family
ID=42433309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910194192A Pending CN101718704A (en) | 2009-11-26 | 2009-11-26 | Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101718704A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519956A (en) * | 2011-12-29 | 2012-06-27 | 北京市药品检验所 | Quick phenolphthalein testing method and test paper utilizing same |
| CN103604805A (en) * | 2013-11-29 | 2014-02-26 | 中山鼎晟生物科技有限公司 | Rapid detection method for phenolphthalein in weight-losing food |
| CN104280385A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Method for rapidly detecting phenolphthalein in health medicine |
| CN104280516A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Rapid detection kit and detection method for phenolphthalein in diet food |
| CN104297237A (en) * | 2014-10-18 | 2015-01-21 | 中山鼎晟生物科技有限公司 | Rapid detection method for phenolphthalein in diet food |
-
2009
- 2009-11-26 CN CN200910194192A patent/CN101718704A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519956A (en) * | 2011-12-29 | 2012-06-27 | 北京市药品检验所 | Quick phenolphthalein testing method and test paper utilizing same |
| CN102519956B (en) * | 2011-12-29 | 2014-08-27 | 北京市药品检验所 | Quick phenolphthalein testing method and test paper utilizing same |
| CN103604805A (en) * | 2013-11-29 | 2014-02-26 | 中山鼎晟生物科技有限公司 | Rapid detection method for phenolphthalein in weight-losing food |
| CN104280385A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Method for rapidly detecting phenolphthalein in health medicine |
| CN104280516A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Rapid detection kit and detection method for phenolphthalein in diet food |
| CN104297237A (en) * | 2014-10-18 | 2015-01-21 | 中山鼎晟生物科技有限公司 | Rapid detection method for phenolphthalein in diet food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Effect of Vaccinium bracteatum Thunb. leaves extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice | |
| Saheed et al. | Toxicological evaluations of Stigma maydis (corn silk) aqueous extract on hematological and lipid parameters in Wistar rats | |
| Sengsuk et al. | Effect of cinnamon supplementation on glucose, lipids levels, glomerular filtration rate, and blood pressure of subjects with type 2 diabetes mellitus | |
| Mohammed et al. | An assessment of selected nutritional, bioactive, thermal and technological properties of brown and red Irish seaweed species | |
| CN101718704A (en) | Test paper for quickly detecting phenothalin, detection kit thereof and detection method thereof | |
| CN108226321B (en) | Fingerprint detection method and fingerprint of fructus piperis longi and fructus piperis longi stomach-ache granules | |
| Mascaro et al. | Effects of Dietary Supplementation with Agaricus sylvaticus Schaeffer on Glycemia and Cholesterol after Streptozotocin‐Induced Diabetes in Rats | |
| Shi et al. | Alpha7-nicotinic acetylcholine receptors involve the imidacloprid-induced inhibition of IgE-mediated rat and human mast cell activation | |
| CN111272904B (en) | Construction method and application of characteristic spectrum of medicine terminalia chebula and fructus chebulae tomentosa | |
| Hao et al. | The inhibitory effects of Yixing black tea extracts on A‐glucosidase | |
| CN102519956B (en) | Quick phenolphthalein testing method and test paper utilizing same | |
| CN101650315B (en) | Quick screening method of phenolphthalein doped in Chinese patent medicine, health products and food for losing weight | |
| Lim et al. | A narrative review of human clinical trials on the impact of phenolic-rich plant extracts on prediabetes and its subgroups | |
| CN103169864B (en) | Zengye Tang granule and preparation method thereof, purposes and detection method | |
| CN103134897A (en) | Rhiaoma iridis tectori capsule detecting method | |
| Sousa et al. | Dissolution test of herbal medicines containing Paullinia cupana: validation of methods for quantification and assessment of dissolution | |
| CN108066481A (en) | A kind of preparation method of antipyretic and detoxicated oral liquid | |
| CN109900827B (en) | Method for identifying radix polygoni multiflori preparata based on one-test-multiple evaluation method | |
| CN108020611A (en) | A kind of method of a variety of biflavone contents in detection gingko leaf preparation | |
| Bloomer et al. | Effect of an aqueous Russian tarragon extract on glucose tolerance in response to an oral dextrose load in non-diabetic men | |
| CN103134896A (en) | Detection method for Chinese materia medica preparation for treating chronic prostatitis | |
| Preechasuk et al. | Effect of Thunbergia laurifolia Herbal Tea on Glucose Homeostasis in Healthy Volunteers: A Single‐Arm Phase I Study | |
| CN104569165B (en) | A kind of detection method peaceful for treating the treating coronary heart disease and angina pectoris compositions Ge Lan heart | |
| CN102204945A (en) | Extraction method of herba cirsii japonici carbonisatum general flavone liquid | |
| Zhang et al. | Analysis of Bitter Almonds and Processed Products Based on HPLC‐Fingerprints and Chemometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20100602 |