CN109900827B - Method for identifying radix polygoni multiflori preparata based on one-test-multiple evaluation method - Google Patents
Method for identifying radix polygoni multiflori preparata based on one-test-multiple evaluation method Download PDFInfo
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Abstract
The invention discloses a method for identifying prepared fleece-flower root based on one test and multiple evaluations, which is verified by methodology and rechecked by provincial level inspection institutions, and the prepared fleece-flower root sample is good in reappearance, thereby indicating that the proposed method is feasible. The one-test-multiple evaluation method which is firstly proposed is subjected to data census analysis in four years, is researched from a laboratory to a decoction piece company, processed products are processed from the same batch of raw products, the processing time of the traditional fleece-flower root processed by nine times of steaming is investigated, the obtained F value limit is feasible, emodin is used as a reference substance, and the fleece-flower root processed completely is obtained when the data show that the F value is not more than 0.6, because the F value is in a stable state below 0.6; the F value is more than or equal to 1.0, which is the biological characteristic, and the F value is between 0.6 and 1.0, which is the processed radix polygoni multiflori sample. The invention solves the problem that the prepared fleece-flower root can not be completely processed or not in appearance. Meanwhile, the method for evaluating the content of the ingredients by one test and multiple tests is low, and provides good support for safe use of the bulk medicinal material prepared fleece-flower root.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine identification, and particularly relates to a method for identifying prepared fleece-flower root based on a one-test-multiple-evaluation method.
Background
Polygoni Multiflori radix is dried root tuber of Polygonum multiflorum Thunb of Polygonaceae, and has effects of removing toxic substance, resolving carbuncle, preventing malaria, and loosening bowel to relieve constipation. The prepared fleece-flower root is a processed product of the fleece-flower root, and the prepared fleece-flower root has completely different efficacies with the fleece-flower root: the prepared fleece-flower root is widely used in the clinical practice of traditional Chinese medicine as a bulk medicinal material, and has the effects of tonifying liver and kidney, benefiting essence and blood, blackening beard and hair, strengthening bones and muscles, eliminating turbid pathogen and reducing blood fat; the fleece-flower root has the effects of detoxifying, resolving carbuncle, preventing malaria and relaxing bowel.
The book of materia medica Hui Yan records that the drug is not used for sleeping for nine times. Not black beans, do not kill their vigor. The processing method of the prepared fleece-flower root refers to Chinese pharmacopoeia 2010 edition and Beijing Chinese herbal medicine processing specifications, and the toxicity of a fleece-flower root processed product is obviously reduced along with the increase of processing time. The report of related subject groups shows that in 30 batches of prepared fleece-flower roots purchased by the research, the experimental results show that 14 batches of control medicinal materials with toxicity close to or even higher than that of raw fleece-flower roots suggest that the prepared products are not effectively processed and attenuated, the risk of clinical liver injury is increased, and the clinical medication safety of patients is seriously threatened. However, the test items of the prepared fleece-flower root and the fleece-flower root in the current pharmacopeia standard are similar, and whether the prepared fleece-flower root is completely processed cannot be distinguished. The reports of hepatotoxicity of prepared fleece flower root are increasing: the Chinese knowledge is searched, and 686 adverse reactions of hepatotoxicity are counted in the literature in the last 5 years. Therefore, the identification of genuine product and non-genuine product is different from the identification of the incompletely processed polygonum multiflorum, because the appearance and character of the incompletely processed polygonum multiflorum cannot be judged whether the processed polygonum multiflorum is completely processed.
The teacher subject group of Luodingqiang bears the national evaluation quality analysis subject of polygonum multiflorum and prepared polygonum multiflorum in 2015, and experiments and comparative literature data show that glycosides are reduced and corresponding aglycones are increased along with the increase of processing time in the processing process of polygonum multiflorum. In 2016, through general survey and analysis of raw polygonum multiflorum and prepared polygonum multiflorum, the 'polygonum multiflorum quality analysis' paper of No. 10 of volume 39 of Chinese medicinal materials, such as Luodingqiang, firstly proposes a 'polygonum multiflorum rule', namely, the ratio F of the content sum of emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside to the content sum of emodin and physcion is more than or equal to 1.0, otherwise, the polygonum multiflorum is prepared.
However, as the subject group further studies in recent years have found that it is not possible to accurately determine whether the processed polygonum multiflorum is completely processed if the F value is less than 1.0, and the hepatotoxicity of incompletely processed polygonum multiflorum still affects the wide application of the medicine. Therefore, the existing method still cannot solve the identification problem of the prepared fleece-flower root.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a method for identifying prepared fleece-flower root based on a one-test-multiple-evaluation method, which can solve the problem that the prior art cannot judge whether the prepared fleece-flower root is completely processed.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:
the invention discloses a method for identifying radix polygoni multiflori preparata based on a one-test-multiple evaluation method, which comprises the following steps:
1) calculating a relative correction factor: accurately measuring emodin, physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside, respectively preparing four reference substance solutions, then adopting an external standard method to obtain standard curves of the four reference substances, and respectively calculating relative correction factors of the physcion, the emodin-8-O-beta-D-glucopyranoside and the physcion-8-O-beta-D-glucopyranoside by taking the emodin as an internal standard;
2) calculating the content of each component: preparing a test solution from prepared polygonum multiflorum powder to be detected, preparing a reference solution from emodin serving as a reference, performing gradient elution by adopting a high performance liquid chromatography to obtain a chromatogram, and calculating the contents of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside according to relative correction factors of corresponding components calculated in the step 1) by taking the peak area of the emodin reference as a reference;
3) calculating the F value to judge whether the prepared fleece-flower root to be detected is completely processed, wherein the F value is calculated according to the formula (1):
emodin-8-O-beta-D-glucopyranoside content + physcion-8-O-beta-D-glucopyranoside content/emodin content + physcion content (1)
Then there are:
when F is less than or equal to 0.6, judging that the prepared fleece-flower root to be detected is completely processed;
when the F is more than or equal to 1.0, judging the crude product of the prepared fleece-flower root to be detected;
and when F is more than 0.6 and less than 1, judging that the prepared fleece-flower root to be detected is incompletely processed fleece-flower root.
Preferably, the change of the F value is related to the processing time, the processing time of the crude polygonum multiflorum is more than or equal to 8 hours, and the F value is less than or equal to 0.6.
Preferably, the high performance liquid chromatography conditions are: octadecylsilane chemically bonded silica is used as a filling agent; acetonitrile is used as a mobile phase A, 0.1% phosphoric acid aqueous solution is used as a mobile phase B, and the detection wavelength is 254 nm.
Further preferably, when gradient elution is performed by high performance liquid chromatography, the change ratio of the mobile phase A to the mobile phase B is as follows:
0-8 min, mobile phase A: 18% -30% of a mobile phase B: 70% -82%;
8-24 min, mobile phase A: 30% -36%, mobile phase B: 64 to 70 percent;
24-28 min, mobile phase A: 36% -80%, mobile phase B: 20% -64%;
28-39 min, mobile phase A: 80% -95%, mobile phase B: 5% -20%;
39-40 min, mobile phase A: 18% -95%, mobile phase B: 5% -82%;
40-45 min, mobile phase A: 18% and mobile phase B: 82 percent.
Preferably, the relative correction factor calculation method in step 1) is as follows: using emodin as internal standard substance, and calculating emodin monomethyl ether, emodin-8-O-beta-D-glucopyranose according to formula (2)Relative correction factor f of glycoside and physcion-8-O-beta-D-glucopyranosidekm；
Wherein A iskArea of emodin peak; wkIs the emodin concentration; a. themPeak areas of other components to be measured; wmThe concentration of other components to be measured; k is emodin, m is other component to be detected, fkIs a correction factor of emodin, fmCorrection factors for other components to be measured. Here, the other components to be tested refer to physcion, emodin-8-O- β -D-glucopyranoside, and physcion-8-O- β -D-glucopyranoside.
Further preferably, the relative retention time and relative correction factors of emodin, physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside are as follows:
preferably, in step 2), the test solution is prepared by adding 50mL of methanol to 1g of the powder of radix Polygoni Multiflori Preparata to be tested, heating and refluxing for 1h, and cooling.
Preferably, in step 2), the control solution is prepared by dissolving emodin in methanol to obtain a solution with a concentration of 30 μ g/ml.
Compared with the prior art, the invention has the following beneficial effects:
the invention discloses a method for identifying fleece-flower root based on one-test-multiple-evaluation method, which comprises the steps of carrying out investigation on processing time of traditional fleece-flower root from a laboratory to a decoction piece company through a large amount of test accumulation and data census analysis, processing products from the same batch of raw products, and obtaining the feasible limit of F value, wherein emodin is used as a reference substance, and data show that the fleece-flower root with the F value not more than 0.6 is completely processed, and the F value is in a stable state below 0.6; the F value is more than or equal to 1.0, which is the biological characteristic, and the F value is between 0.6 and 1.0, which is the processed radix polygoni multiflori sample. The method can clearly identify the prepared fleece-flower root, can accurately judge whether the prepared fleece-flower root is completely processed, and provides an effective identification means for ensuring the clinical safe use of the large amount of medicinal material prepared fleece-flower root. Meanwhile, the method only uses emodin as a reference substance, so that the detection cost is very low, and the resources are saved.
Drawings
FIG. 1 is a graph showing F value change curves of 6 lots of Polygonum multiflorum samples.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The invention is described in further detail below with reference to the accompanying drawings:
comparing the current prepared fleece-flower root standard with the quality standard of raw fleece-flower root
The standard comparison is shown in table 1.
TABLE 1 comparison of the existing standards for Polygonum multiflorum and prepared Polygonum multiflorum
According to standard comparison, the test items are similar, the polygonum multiflorum and the prepared polygonum multiflorum contain bound anthraquinone and free anthraquinone, the content difference between the polygonum multiflorum and the prepared polygonum multiflorum in different production areas is large, and the content measurement items of part of raw products also meet the content measurement limit of the prepared polygonum multiflorum, namely whether the prepared polygonum multiflorum is completely processed cannot be effectively distinguished.
Identification of prepared fleece-flower root by two-test and one-test multi-evaluation method
1. The instrument comprises the following steps: BP211D electronic analytical balance (sydoris, germany); ME204 (mettler-toledo, switzerland); LC-2030C 3D high performance liquid chromatograph (Shimadzu corporation, Japan); LC-30AD high performance liquid chromatograph (Shimadzu corporation, Japan); agilent 1260Infinity II high performance liquid chromatograph (Agilent Inc., USA); empower chromatography working software.
2. Materials and reagents: emodin reference (110756 201512 with purity of 98.7%) and physcion reference (110758 201415 with purity of 99.1%) provided by the Chinese institute of food and drug assay; emodin-8-O-beta-D-glucopyranoside reference substance (purity 97.0%) and emodin methyl ether-8-O-beta-D-glucopyranoside reference substance (purity 98%) were purchased from Shanghai Hotan Biotechnology corporation; acetonitrile and methanol are used as chromatographic purity, and water is used as ultrapure water; the other reagents are analytically pure. Acetonitrile is chromatographically pure, others are analytically pure, and water is deionized water.
3. Sample source: the prepared fleece-flower root and fleece-flower root samples are tested according to the national special draft test plan and the revision task of 2020 version Chinese pharmacopoeia decoction pieces and according to the appendix 0211 of the four parts of the 2015 version Chinese pharmacopoeia in the sampling method of medicinal materials and decoction pieces. At least 150g of each batch of decoction pieces is sampled. Extracting 66 batches of polygonum multiflorum decoction pieces from 22 provinces, 5 autonomous regions and 31 provincial administrative regions including 4 direct municipalities except hong Kong, Macao and Taiwan, wherein the number of the polygonum multiflorum decoction pieces relates to 51 manufacturers and is distributed in 19 provinces; 106 batches of prepared polygonum multiflorum decoction pieces relate to 100 families of production enterprises and are distributed in 26 provinces.
4. Method of producing a composite material
1) Test solution preparation: weighing about 1g of the powder (sieved by a sieve IV), accurately weighing, placing in a conical flask with a plug, accurately adding 50ml of methanol, weighing, heating and refluxing for 1h, taking out, cooling, and taking the subsequent filtrate as a test sample. Taking emodin reference substance, precisely weighing, and adding methanol to obtain solution containing 30 μ g per 1ml as reference substance solution.
2) High performance liquid chromatography conditions: octadecylsilane chemically bonded silica was used as a filler in accordance with a high performance liquid chromatography (China pharmacopoeia 2015, four parts of the year 0512); acetonitrile was used as mobile phase A, and 0.1% phosphoric acid aqueous solution was used as mobile phase B, and gradient elution was carried out as specified in the following table to detect a wavelength of 254 nm. The number of theoretical plates is not less than 5000 calculated according to emodin peak.
When the high performance liquid chromatography is adopted for gradient elution, the change ratio of the mobile phase A to the mobile phase B is as follows:
0-8 min, mobile phase A: 18% -30% of a mobile phase B: 70% -82%;
8-24 min, mobile phase A: 30% -36%, mobile phase B: 64 to 70 percent;
24-28 min, mobile phase A: 36% -80%, mobile phase B: 20% -64%;
28-39 min, mobile phase A: 80% -95%, mobile phase B: 5% -20%;
39-40 min, mobile phase A: 18% -95%, mobile phase B: 5% -82%;
40-45 min, mobile phase A: 18% and mobile phase B: 82 percent.
3) Measurement and calculation: and (3) taking 10 mu l of each of the test solution and the reference solution, injecting into a liquid chromatograph, and recording a chromatogram. The peak area of emodin reference substance is used as reference, the contents of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside are calculated according to the corresponding correction factors in the following table, and the relative retention time of the chromatographic peak of the component to be detected and the peak positions of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside is within +/-5% of the specified value (if the relative retention time deviates more than 5%, the emodin and polygonum multiflorum reference medicinal material or the corresponding reference substance are used for confirmation).
4) Calculation of relative correction factors:
preparation of control: accurately weighing 10.08mg of physcion reference substance, placing in a 100mL measuring flask, adding methanol to dissolve, fixing volume to scale, and shaking up to obtain No. 1 stock solution; accurately weighing 7.20mg of emodin reference substance, 11.16mg of emodin-8-O-beta-D-glucopyranoside and 9.92mg of physcion-8-O-beta-D-glucopyranoside, placing the components into a 100mL measuring flask, adding methanol for dissolving, fixing the volume to scale, shaking up to obtain a No. 2 stock solution. Precisely measuring 1mL of the stock solution No. 1 and 1mL of the stock solution No. 2 respectively, placing in a 50mL measuring flask, diluting with methanol to scale, and shaking up to obtain a reference solution No. 1; precisely measuring No. 1 stock solution and No. 2 stock solution 2mL respectively, placing in a 50mL measuring flask, diluting with methanol to scale, and using as No. 2 reference solution; precisely measuring the No. 1 stock solution and the No. 2 stock solution respectively by 2mL, placing the two solutions into a 25mL measuring flask, and metering the volume to scale by using methanol to obtain a No. 3 reference solution; precisely measuring 1mL of the two stock solutions No. 1 and No. 2 respectively, placing the two stock solutions into a 10mL measuring flask, and diluting the two stock solutions to a scale with methanol to obtain a No. 4 reference solution; precisely measuring No. 1 and No. 2 stock solutions respectively, placing 2mL of the stock solutions into a 10mL measuring flask, and diluting with methanol to a scale to obtain No. 5 reference solution; precisely measuring 2mL of the two stock solutions No. 1 and No. 2 respectively, placing the two stock solutions into a 5mL measuring flask, and diluting the two stock solutions to a scale with methanol to obtain a No. 6 reference solution; no. 1 stock solutions were precisely measured, 5mL of each of No. 2 stock solutions were placed in 10mL measuring flasks, and diluted to the scale with methanol to serve as No. 7 control solutions.
Preparation of a test solution: taking a proper amount of a test sample, crushing, sieving by a fourth sieve, precisely weighing 1g of test sample powder, placing in a triangular flask, precisely adding 50mL of methanol, weighing, heating and refluxing for 1 hour, preventing cold, supplementing the lost weight with methanol, filtering, and taking a subsequent filtrate as a test sample solution.
Linear range: precisely sucking 10 μ L of each of the above mixed control solutions 1,2,3,4,5,6, and 7, injecting into a liquid chromatograph, measuring according to the above chromatographic conditions, recording the chromatographic peak area, and drawing a standard curve with the sample volume (X, μ g) as abscissa and the peak area (Y) as ordinate. The 4 standard curves were each well linear over the linear range, as shown in table 2:
TABLE 2 Standard curves for the four Components
Precisely sucking 10 mu L of No. 6 mixed reference substance solution, injecting samples for 3 times, and recording the peak area and retention time of the chromatogram. Relative correction factors of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside are calculated according to the following formula (2) by taking emodin as an internal standard and emodin as an internal standard.
Wherein A iskArea of emodin peak; wkIs the emodin concentration; a. themPeak areas of other components to be measured; wmThe concentration of other components to be measured; k is emodin, m is other component to be detected, fkIs a correction factor of emodin, fmCorrection factors for other components to be measured. As a result, relative correction factors of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside were 0.92, 2.27 and 2.04, respectively. The relative retention times of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside are respectively 1.11, 0.47 and 0.61 by calculating that the retention time of emodin is 1.00。
The relative retention times and relative correction factors are shown in table 3 below:
TABLE 3 relative retention time and relative correction factor
Meanwhile, the precision test, the stability test, the repeatability test and the recovery rate test all accord with the precision requirement of equipment, the stability at room temperature is good, the repeatability is good, and the measurement result is stable.
5) Correction factor durability review
The influence of different sample injection volumes, different chromatographic columns and different instruments on the correction factor is inspected, and the RSD is less than 3 percent, which is shown in tables 4 and 5:
TABLE 4 correction factor for different sample volumes
TABLE 5 correction factors for different chromatographic columns of different instruments
In conclusion, the relative correction factor of the invention has better durability and reliable result after durability test.
6) And F value calculation:
emodin-8-O-beta-D-glucopyranoside content + physcion-8-O-beta-D-glucopyranoside content/emodin content + physcion content (1)
When F is less than or equal to 0.6, judging that the prepared fleece-flower root to be detected is completely processed;
when the F is more than or equal to 1.0, judging the crude product of the prepared fleece-flower root to be detected;
and when F is more than 0.6 and less than 1, judging that the prepared fleece-flower root to be detected is incompletely processed fleece-flower root.
According to the above method, the following experiments were carried out:
10 batches of crude polygonum multiflorum are purchased from the market, and the polygonum multiflorum samples are steamed for different time periods, and the measurement results are shown in table 6:
TABLE 6 comparison of the F-value results of the one-test-multiple-evaluation method and the external standard method
The results in table 6 show that according to the analysis between the groups, the one-side multi-evaluation method and the external standard method have no significant difference, but only one reference substance of the emodin is used, the detection cost of the reference substance is less than 100 yuan, and the detection cost is greatly saved.
Example 1 (determination of F value limits)
Selecting representative 6 batches of crude Polygonum multiflorum Thunb, stewing the crude Polygonum multiflorum Thunb with black bean juice for 8 hours with F value of 1.03-9.61, steaming for different time (0.2-0.4MPa), and processing by Shaanxi Guangji Tang pharmaceutical group Limited company. The measurements were carried out according to established methods and the results are shown in Table 7:
TABLE 76 batches of crude Polygonum multiflorum Thunb steaming time and F value
From the above table 7 and fig. 1, it can be seen that the change of F value is directly related to the processing time, and after the crude polygonum multiflorum is steamed for 8 hours, i.e. the F value is below 0.6, the F value becomes more and more stable. Therefore, the invention provides that the F value is not more than 0.6, namely the prepared fleece-flower root is completely processed.
Example 2 (verification of F value Limit)
The data of 66 batches of crude polygonum multiflorum are generally checked, the range of the F value is verified, and the result is shown in table 8:
TABLE 866 batches of crude Polygonum multiflorum F value determination results
The F value is found to be more than or equal to 1.0 by the data census analysis of the F values of 66 batches of crude polygonum multiflorum. The F value is more than or equal to 1.0, which indicates that the crude product has the characteristics of crude product, but the crude product has completely different efficacies with the processed product, and the literature data proves that the damage of the crude product and the processed radix polygoni multiflori on hepatotoxicity is obviously higher than that of the processed radix polygoni multiflori.
Example 3 (verification of F value Limit)
The data of 106 batches of prepared fleece-flower root are generally checked, and the range of F value is analyzed and verified, and the result is shown in Table 9:
TABLE 9106 batches of crude radix Polygoni Multiflori Preparata F value determination results
The processing conditions of the prepared polygonum multiflorum samples in the market can be clearly distinguished through the established limit of the F value of 0.6. According to the proposed method, the qualification rate is 84.9%, 10 batches of samples have the property of raw products, and 6 batches of prepared polygonum multiflorum are not completely processed.
Example 4
The results of the method are rechecked and detected by 3 batches of prepared polygonum multiflorum samples sent to Heilongjiang food and drug inspection and detection, and the reproducibility of the method can be confirmed according to the issued inspection reports (the numbers of the reports are HL2017Q4289, HL2017Q4290 and HL2017Q 4290).
The above-mentioned contents are only for illustrating the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and any modification made on the basis of the technical idea of the present invention falls within the protection scope of the claims of the present invention.
Claims (8)
1. The method for identifying the prepared fleece-flower root based on the one-test-multiple-evaluation method is characterized by comprising the following steps of:
1) calculating a relative correction factor: accurately measuring emodin, physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside, respectively preparing four reference substance solutions, then adopting an external standard method to obtain standard curves of the four reference substances, and respectively calculating relative correction factors of the physcion, the emodin-8-O-beta-D-glucopyranoside and the physcion-8-O-beta-D-glucopyranoside by taking the emodin as an internal standard;
2) calculating the content of each component: preparing a test solution from prepared polygonum multiflorum powder to be detected, preparing a reference solution from emodin serving as a reference, performing gradient elution by adopting a high performance liquid chromatography to obtain a chromatogram, and calculating the contents of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside according to relative correction factors of corresponding components calculated in the step 1) by taking the peak area of the emodin reference as a reference;
3) calculating the F value to judge whether the prepared fleece-flower root to be detected is completely processed, wherein the F value is calculated according to the formula (1):
(emodin-8-O-beta-D-glucopyranoside content + physcion-8-O-beta-D-glucopyranoside content)/(emodin content + physcion content) (1)
Then there are:
when F is less than or equal to 0.6, judging that the prepared fleece-flower root to be detected is completely processed;
when the F is more than or equal to 1.0, judging the crude product of the prepared fleece-flower root to be detected;
and when F is more than 0.6 and less than 1, judging that the prepared fleece-flower root to be detected is incompletely processed fleece-flower root.
2. The method of claim 1, wherein the change in F value is related to the processing time, the processing time of the raw polygonum multiflorum is not less than 8 hours, and the F value is not more than 0.6.
3. The method for identifying polygonum multiflorum thunb based on the one-test-multiple-evaluation method of claim 1, wherein the high performance liquid chromatography conditions are: octadecylsilane chemically bonded silica is used as a filling agent; acetonitrile is used as a mobile phase A, 0.1% phosphoric acid aqueous solution is used as a mobile phase B, and the detection wavelength is 254 nm.
4. The method for identifying P.multiflora Thunb based on one-test-multiple-evaluation method according to claim 3, wherein the ratio of change of mobile phase A to mobile phase B in gradient elution by HPLC is as follows:
0-8 min, mobile phase A: 18% -30% of a mobile phase B: 70% -82%;
8-24 min, mobile phase A: 30% -36%, mobile phase B: 64 to 70 percent;
24-28 min, mobile phase A: 36% -80%, mobile phase B: 20% -64%;
28-39 min, mobile phase A: 80% -95%, mobile phase B: 5% -20%;
39-40 min, mobile phase A: 18% -95%, mobile phase B: 5% -82%;
40-45 min, mobile phase A: 18% and mobile phase B: 82 percent.
5. The method for identifying polygonum multiflorum thunb based on one-test-multiple-evaluation method according to claim 1, wherein the relative correction factor in step 1) is calculated by: using emodin as internal standard substance, calculating relative correction factor f of physcion, emodin-8-O-beta-D-glucopyranoside and physcion-8-O-beta-D-glucopyranoside according to formula (2)km；
Wherein A iskArea of emodin peak; wkIs the emodin concentration; a. themPeak areas of other components to be measured; wmThe concentration of other components to be measured; k is emodin, m is other component to be detected, fkIs a correction factor of emodin, fmFor correcting other components to be measuredAnd (4) adding the active ingredients.
6. The method of claim 5, wherein the relative retention times and relative correction factors of emodin, physcion, emodin-8-O- β -D-glucopyranoside and physcion-8-O- β -D-glucopyranoside are as follows:
7. the method of claim 1, wherein the test solution is prepared by adding 50mL of methanol to 1g of the powder of radix Polygoni Multiflori Preparata to be tested, heating and refluxing for 1h, and cooling in step 2).
8. The method of claim 1, wherein the control solution is prepared by dissolving emodin in methanol to a concentration of 30 μ g/ml in step 2).
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