CN101717796A - Method for processing banana stalks with enzyme to produce fuel ethanol - Google Patents
Method for processing banana stalks with enzyme to produce fuel ethanol Download PDFInfo
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- CN101717796A CN101717796A CN200910194348A CN200910194348A CN101717796A CN 101717796 A CN101717796 A CN 101717796A CN 200910194348 A CN200910194348 A CN 200910194348A CN 200910194348 A CN200910194348 A CN 200910194348A CN 101717796 A CN101717796 A CN 101717796A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
本发明公开了一种酶法处理香蕉杆发酵生产燃料酒精的方法，本发明采用不同种类纤维素酶对香蕉杆处理，该方法首先取新鲜香蕉杆切碎，灭菌，按比例加入经稀释的纤维素酶液对香蕉杆进行水解处理；然后，加入经活化的耐高温酿酒酵母，搅拌均匀后，置发酵设备中发酵，发酵醪液采用常压蒸馏法分离提纯酒精；本发明采用廉价或无价的香蕉杆为原材料，采用不同种类纤维素酶对香蕉杆降解和糖化处理，处理工艺简单易行，提高糖化率；后续发酵过程采用半连续发酵工艺，发酵过程中可发酵糖利用率增大，发酵生产燃料酒精的产率高，从而降低酒精的生产成本，适合燃料酒精的大规模工业化生产。The invention discloses a method for enzymatically treating banana stalks to ferment and produce fuel alcohol. The invention adopts different kinds of cellulase to treat banana stalks. In the method, fresh banana stalks are first chopped, sterilized, and diluted The cellulose enzyme solution is used to hydrolyze the banana stems; then, the activated high-temperature-resistant Saccharomyces cerevisiae is added, stirred evenly, and fermented in fermentation equipment, and the fermented mash is separated and purified by atmospheric distillation; the present invention adopts cheap or non-toxic The cheap banana stems are used as raw materials, and different types of cellulase are used to degrade and saccharify banana stems. The treatment process is simple and easy, and the saccharification rate is improved; the subsequent fermentation process adopts a semi-continuous fermentation process, and the utilization rate of fermentable sugar increases during the fermentation process. , the yield of fuel alcohol produced by fermentation is high, thereby reducing the production cost of alcohol, and is suitable for large-scale industrial production of fuel alcohol.
Description
Technical field
The present invention relates to a kind of method of processing banana stalks with enzyme to produce fuel ethanol, belong to biomass energy and agronomic crop fibre comprehensive utilization technique field.
Background technology
Biomass material existence worldwide is extensive, is that raw material degraded preparation fuel alcohol has substituted conventional energy resources at present with the lignocellulose.Main cellulose, hemicellulose and xylogen in the biomass material material.Wherein, Mierocrystalline cellulose content in many biomass materials is the highest, can change cellobiose into by degraded, and further saccharification is a monose, can be used to prepare fuel alcohol.The biological material of main research comprises plant straw, chaff shell, timber, paper, cotton, fiber crops and agricultural byproducts etc. at present, be characterized in existing extensively at nature, content of cellulose is all more than 40%, belonging to cheap or priceless starting material, is human most economical, the most sustainable renewable natural resources.
Biomass are world's fourth largest energy.To be sun power be stored in a kind of energy form in the biology with the chemical energy form to biomass energy, and it is a kind of renewable energy source, and inexhaustible, nexhaustible characteristics are arranged, and is the important component part of the world energy sources strategy of sustainable development.Compare with numerous renewable energy sources, biomass energy has advantages such as reserves are big, distribution is wide, environment protecting is obvious, and very big potentiality to be exploited is arranged.After world oil crisis took place, the oil supply amount was had a strong impact on, and the research of fuel substitute earns widespread respect.Fuel alcohol because it has safely, cleans, can partly or entirely replace characteristics such as fossil energy, more and more is subjected to the welcome of various countries as a kind of potential energy source.Utilizing biomass to produce the important channel that fuel alcohol is the development and use biomass energy, also is the important directions of Production of Fuel Ethanol in future.Fuel alcohol is a raw material with cereal, molasses, fruit juice, grape wine, whey etc. mainly at present, and fermentation, distillation make.But be subjected to the quick growth of global population, cultivated area reduces year by year, the influence that the price of grains such as cereal is more and more higher, and the Production of Fuel Ethanol cost is high always.With the alternate energy source of alcohol as fossil energy, its key is to reduce the production cost of alcohol.Therefore, utilizing the raw material of the lower biomass of cost as Alcohol Production, develop biomass to alcohol conversion process more efficiently, is important from now on research direction.
China's banana planting area has 15～200,000 hectares, accounts for 8% of world's banana planting total area, is very huge as the amount of banana byproduct treat banana stalk.Treat banana stalk is as a kind of important biomass energy, and except that part was utilized, the limit, field is burned or abandoned to the overwhelming majority, and this has not only caused the waste of resource, returns environment and brings pollution.At present many in the world countries have carried out the research that utilizes biomass to produce fuel alcohol, developed country such as the U.S., Brazil even realized utilizing biomass to produce the suitability for industrialized production of fuel alcohol.The many biomass materials of present domestic research have cereal, potato class, corn cob, corn stalk.Utilize treat banana stalk to produce fuel alcohol and reported the method (number of patent application 200810027458.0) of producing fuel alcohol with chemical method and microorganism to comprehensively treat banana stalk.The technology of preparation of cellulose alcohol mainly comprises pre-treatment of raw material at present, and the hydrocellulose hemicellulose is a fermentable sugars, utilizes fermentable saccharide fermentative production alcohol several stages.At present common pretreatment process comprises physics method, chemical method, and vapour is quick-fried etc., and wherein physics forensic chemistry method exists reductive hydrolysis sugar amount not high to some extent, or problem such as environmental pollution is serious, is difficult to be applied to large-scale industrial production.
Summary of the invention
The purpose of this invention is to provide a kind of enzymatic treatment treat banana stalk and produce the method for fuel alcohol, filter out and a kind ofly be suitable for most the enzyme liquid of treat banana stalk of degrading, and optimize hydrolysis, fermentation condition.
The technical solution adopted for the present invention to solve the technical problems is:
The method steps of processing banana stalks with enzyme to produce fuel ethanol is as follows: at first get the chopping of fresh treat banana stalk, and long 1～3cm, wide 0.2～0.5cm, sterilization adds diluted cellulase solution in proportion to the treat banana stalk processing that is hydrolyzed; Then, add activated high temperature resistant yeast saccharomyces cerevisiae, after stirring, put in the fermentation equipment and ferment, fermentation liquid adopts atmospheric distillation to separate purification alcohol.
Above-mentioned cellulase solution is formed by the cellulase dilution, and cellulase is cellulase NS50013 and saccharifying enzyme NS50010, and the NS50013 of adding and NS50010 mass ratio are 10: 1.
The diluting soln of above-mentioned cellulase solution is a sterilized water, and the volume ratio of cellulase and sterilized water is 1: 60～100.
Above-mentioned treat banana stalk: cellulase solution: the zymic mass ratio is 1: 0.2～0.6: 0.01～0.05.
The said hydrolyzed condition is that hydrolysis time is 24～36h, pH4.5～6.5, and hydrolysis temperature is 45～60 ℃.
Above-mentioned fermentation condition is that fermentation time is 48～96h, pH8～10, and leavening temperature is 25～35 ℃.
Described cellulase NS50013 and saccharifying enzyme NS50010 add simultaneously.
Simple with enzymatic treatment treat banana stalk technology, environmental pollution is little, the productive rate height of reductive hydrolysis sugar.The key problem of this technology is the determining of consumption, hydrolysising condition and fermentation condition of cellulase solution.This process using high active cellulase, comprise cellulase NS50013 and saccharifying enzyme NS50010, NS50013 can be polysaccharide and cellobiose etc. with the cellulose degradation material, and NS50010 is degraded to glucose with polysaccharide, cellobiose, provides more monose to be used for follow-up zymotechnique.Wherein, the enzyme activity of cellulase NS50013 is 700EGU/g, and condition is pH4.5～6.5,45～60 ℃ of temperature, and consumption is a mass ratio 2～6%; The enzyme activity of saccharifying enzyme NS50010 is 250CbU/g, condition is pH4.5～6.5,45～60 ℃ of temperature, consumption is a mass ratio 0.2～0.6%, data are substrate with the distillation cereal through pre-treatment, the repetition of enzyme activity condition is substrate with the treat banana stalk, and the treat banana stalk degradation effect is obvious, the ethanol production height.
The beneficial effect that the inventive method is compared with background technology:
The present invention is starting material with cheap or priceless treat banana stalk, adopted highly active cellulase that treat banana stalk is handled, conversion coefficient in the technology is improved, reduced preprocessing process simultaneously, thereby make the raising of ethanol production become possibility, for realizing utilizing biomass large-scale industrial production fuel alcohol that another kind of approach is provided.This technology preprocessing process is simple, shortens the hydrolysis time of treat banana stalk in the hydrolytic process, shortens to 1～2 day in 5～7 days; Conversion coefficient improves, for zymotechnique provides more fermentable sugars; The operational path of this technology formation cycle weak point, hydrolysis and saccharification rate height, the low treat banana stalk fermentative production alcohol that consumes energy.This technology has overcome additive method treat banana stalk has been carried out the complex process that pre-treatment exists, and conversion coefficient is not high, the deficiency that hydrolysis time is long.
Embodiment
Embodiment 1:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 4.5 with 60 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 45 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 24h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 1.0g activation, regulates pH8.0, in the fermenting container of packing into after stirring.Under 25 ℃ condition, sealing and fermenting 48h; At last, remove by filter dry material,, can obtain the alcohol 2.5ml of concentration more than 80%, be equivalent to about 87.3% of theoretical yield fermented liquid air distillation 3 times.
Embodiment 2:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 5.0 with 100 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 50 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 24h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 2.0g activation, regulates pH8.0, in the fermenting container of packing into after stirring.Under 30 ℃ condition, sealing and fermenting 64h; At last, remove by filter dry material,, can obtain the alcohol 2.3ml of concentration more than 80%, be equivalent to about 80.3% of theoretical yield fermented liquid air distillation 3 times.
Embodiment 3:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 5.5 with 60 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 55 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 36h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 3.0g activation, regulates pH9.0, in the fermenting container of packing into after stirring.Under 35 ℃ condition, sealing and fermenting 72h; At last, remove by filter dry material,, can obtain the alcohol 2.7ml of concentration more than 80%, be equivalent to about 94.3% of theoretical yield fermented liquid air distillation 3 times.
Embodiment 4:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 6.0 with 80 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 60 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 36h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 4.0g activation, regulates pH10.0, in the fermenting container of packing into after stirring.Under 25 ℃ condition, sealing and fermenting 96h; At last, remove by filter dry material,, can obtain the alcohol 2.6ml of concentration more than 80%, be equivalent to about 90.8% of theoretical yield fermented liquid air distillation 3 times.
Embodiment 5:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 5.0 with 60 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 55 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 48h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 4.0g activation, regulates pH9.0, in the fermenting container of packing into after stirring.Under 30 ℃ condition, sealing and fermenting 64h; At last, remove by filter dry material,, can obtain the alcohol 2.4ml of concentration more than 80%, be equivalent to about 83.8% of theoretical yield fermented liquid air distillation 3 times.
Embodiment 6:
At first, get the fresh treat banana stalk of 100.0 ± 0.5g and pulverize, add the cellulase solution of 100ml, regulate pH value 6.0 with 100 times of sterilized water dilutions, cellulase in the cellulase solution is cellulase NS50013 and saccharifying enzyme NS50010, and the mass ratio of NS50013 and NS50010 is 10: 1.Then, putting temperature is 55 ℃, and rotating speed is in the shaking table of 90r/min behind the concussion 24h, adds the high temperature resistant yeast saccharomyces cerevisiae after the 5.0g activation, regulates pH10.0, in the fermenting container of packing into after stirring.Under 35 ℃ condition, sealing and fermenting 96h; At last, remove by filter dry material,, can obtain the alcohol 2.7ml of concentration more than 80%, be equivalent to about 94.3% of theoretical yield fermented liquid air distillation 3 times.
Claims (6)
1. the method for a processing banana stalks with enzyme to produce fuel ethanol, it is characterized in that this method steps is as follows: at first get fresh treat banana stalk chopping, long 1～3cm, wide 0.2～0.5cm, sterilization adds diluted cellulase solution in proportion to the treat banana stalk processing that is hydrolyzed; Then, add activated high temperature resistant yeast saccharomyces cerevisiae, after stirring, put in the fermentation equipment and ferment, fermentation liquid adopts atmospheric distillation to separate purification alcohol.
2. method according to claim 1 is characterized in that: above-mentioned cellulase solution is formed by the cellulase dilution, and cellulase is cellulase NS50013 and saccharifying enzyme NS50010, and the NS50013 of adding and NS50010 mass ratio are 10: 1.
3. method according to claim 2 is characterized in that: the diluting soln of above-mentioned cellulase solution is a sterilized water, and the volume ratio of cellulase and sterilized water is 1: 60～100.
4. method according to claim 1 is characterized in that: above-mentioned treat banana stalk: cellulase solution: the zymic mass ratio is 1: 0.2～0.6: 0.01～0.05.
5. method according to claim 1 is characterized in that: the said hydrolyzed condition is that hydrolysis time is 24～36h, pH4.5～6.5, and hydrolysis temperature is 45～60 ℃.
6. method according to claim 1 is characterized in that: above-mentioned fermentation condition is that fermentation time is 48～96h, pH8～10, and leavening temperature is 25～35 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910194348A CN101717796A (en) | 2009-12-01 | 2009-12-01 | Method for processing banana stalks with enzyme to produce fuel ethanol |
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| CN200910194348A CN101717796A (en) | 2009-12-01 | 2009-12-01 | Method for processing banana stalks with enzyme to produce fuel ethanol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399394A (en) * | 2016-12-11 | 2017-02-15 | 广西中粮生物质能源有限公司 | Method for preparing ethanol from banana trees |
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2009
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399394A (en) * | 2016-12-11 | 2017-02-15 | 广西中粮生物质能源有限公司 | Method for preparing ethanol from banana trees |
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Open date: 20100602 |