CN101709273B - Germanium-enriched yeast and preparation method thereof - Google Patents
Germanium-enriched yeast and preparation method thereof Download PDFInfo
- Publication number
- CN101709273B CN101709273B CN2009101942127A CN200910194212A CN101709273B CN 101709273 B CN101709273 B CN 101709273B CN 2009101942127 A CN2009101942127 A CN 2009101942127A CN 200910194212 A CN200910194212 A CN 200910194212A CN 101709273 B CN101709273 B CN 101709273B
- Authority
- CN
- China
- Prior art keywords
- germanium
- yeast
- enriched
- dry
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses germanium-enriched yeast and a preparation method thereof. The germanium-enriched yeast is characterized by adding inorganic germanium solution in the process of crushing the wall of dried yeast by extrusion swelling by using an extrusion spray exploding machine and chelating the inorganic germanium solution with the yeast content to prepare the germanium-enriched yeast. The invention is simple in technology, convenient in operation, good in continuity, large in treatment capacity and easy to realize industrialization. The obtained yeast is high in content of organic germanium, stable in quality, safe and basically invariant in active components. The invention can be applied to comprehensive utilization of waste saccharomyces cerevisiae, thus changing wastes into valuables, reducing environmental pollution, increasing the added value of products and increasing the economic benefits of beer enterprises.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to rich germanium method of a kind of zymic and application.
Background technology
Germanium is a kind of highly bioactive trace element that has, and the pharmacological action widely that compound had and the potentiality of curing the disease of germanium are inseparable with elements germanium.The biological effect of germanium mainly shows cell growth and metabolism promoter action, immunoregulation effect, and the free radical scavenging effect, antimutagenic effect, the oxygen coalescence effect is with mutual antagonistic action of other elements etc.
Because the ge content in the natural food is generally lower, there is not physiological function basically and the toxicity of inorganic Ge is very high.Though the organic germanium toxicity of chemosynthesis is lower, but the safety that is used in the food still has many query parts, by biotransformation method inorganic Ge is changed into similar naturally occurring organic germanium form, can improve the physiologically active and the specific absorption of germanium and significantly reduce its toxicity, the security that is used on the food is higher.
Yeast has the rich germanium ability of height, inorganic Ge can be converted into organic germanium, can produce germanium-enriched yeast with special process, adds in the food source of trace element germanium as a supplement to.The abundant nutrition of germanium-enriched yeast collection organic germanium biological activity and yeast itself is one, has both had the functions such as antitumor, immunomodulatory of germanium, contains nutritional factor such as rich in protein and mineral substance again, can be widely used in protective foods, pharmaceutical industries etc.
Existing germanium-enriched yeast production adds germanium dioxide (GeO exactly in the yeast culture process
2) etc. the inorganic Ge of form, make it to be converted into the organic germanium form and enrichment and a kind of microbial fermentation product of producing in yeast.But this method fermentative production cycle is longer, the cost height, and suitability for industrialized production is difficulty comparatively.
Summary of the invention
An object of the present invention is to provide a kind of germanium-enriched yeast.
Another object of the present invention is to overcome technological deficiency and the technical barrier that the rich germanium amount of existing yeast is low, fermentation period is long, cost is high, suitability for industrialized production is difficult, provide a kind of zymic rich germanium method, not in the yeast culture process, to carry out, but directly utilize dry yeast production to prepare germanium-enriched yeast, production cycle is shorter, cost is lower, and contains organic germanium amount height.
Purpose of the present invention is achieved by the following technical programs:
A kind of germanium-enriched yeast is provided, dry yeast is carried out in the extruding puffing broken wall treatment process adding inorganic Ge solution and yeast content chelating prepares.The extruding puffing broken wall treatment can utilize extruder to carry out.Described inorganic Ge is germanium dioxide (GeO
2).
The preparation method of described germanium-enriched yeast specifically may further comprise the steps:
(1) the dry yeast removal of impurity of sieving;
(2) add germanium solution;
(3) extruding puffing;
(4) washing removes inorganic Ge;
(5) be drying to obtain germanium-enriched yeast.
The compound method of the described germanium solution of step (2): with 8～16g germanium dioxide: the ratio of 50ml distilled water adds in the distilled water germanium dioxide fully that dissolving back adjust pH is 5.0～7.0.The pH value is 5.0～7.0 to help the dissolving of germanium dioxide and help the generation of chelatropic reaction.
The described germanium solution of step (2) is according to 30～200ml germanium solution: the ratio of 1000g dry yeast be added into dry yeast.
The present invention is by a large amount of experimental summaries, and the preferred expanded condition of step (3) is: adopt the quick-fried machine of extruding spray, swelling temperature: 125 ℃～140 ℃; Input speed: 200～300g/min; Screw speed: 15～30Hz; Rate of water make-up: 5～80g/100g dry yeast.
The described washing of step (4) removes inorganic Ge preferably with the centrifugal 15min of 3500r/min behind the yeast of the expanded back of pure water washing.
The described drying of step (4) can adopt common process, and the present invention is dry 24h under 50 ℃ of conditions of temperature preferably.
The present invention provides the application of described method simultaneously, specifically is to be applied to utilize beer waste yeast to produce germanium-enriched yeast.
The present invention is by the optimization to wall-breaking method and condition, make yeast in broken wall, realize chelatropic reaction, yeast sporoderm-broken rate height, the yeast content is disengaged, its effective constituent is constant substantially, solves well because of the difficult difficult problem that can't industrialization processing of yeast broken wall.Utilize the production method production germanium-enriched yeast production cycle of the present invention shorter, cost is lower, and the organic germanium content height.
The inventive method can be applicable to similar beer waste yeast comprehensive utilization aspects such as brew-house, turns waste into wealth, and reduces environmental pollution, has increased value-added content of product, has improved economic benefit of enterprises.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.Reagent and equipment are commercial conventional products.
Embodiment 1
Concrete operations step of the present invention is as follows:
Get the useless dry yeast of brew-house, cross the 80 mesh sieve removal of impurity;
In 50ml distilled water, add 10g germanium dioxide (GeO2), fully after the dissolving, use
It is 5.8 that potassium hydroxide is transferred the pH value of germanium solution;
(3) according to the 50ml germanium solution: the ratio of 1000g dry yeast is added into dry yeast after step (1) is sieved with germanium solution;
(4) dry yeast that step (3) has been added germanium solution carries out expandedly with the quick-fried machine of extruding spray, and the quick-fried machine of extruding spray can adopt the quick-fried machine of SPJ-40 extruding spray, Shaanxi De Ai Food Co., Ltd make or Agricultural University Of South China's food in the development of machining at low temperature research department.Expanded condition is:
Swelling temperature: 130 ℃
Input speed: 250g/min
Screw speed: 20Hz
Rate of water make-up: 5g/100g dry yeast
(5) yeast after the puffing washs three times with pure water, the centrifugal 15min of 3500r/min;
(6) washing get after centrifugal be deposited in 50 ℃ down dry 24h promptly get germanium-enriched yeast.
The dry yeast sporoderm-broken rate is 75%; Germanium-enriched yeast organic germanium amount is 1240 μ g/g dry yeast after the centrifuge washing drying.
Embodiment 2
Concrete operations step of the present invention is as follows:
(1) gets beer waste yeast, the removal of impurity of sieving; Present embodiment is preferably crossed 80 mesh sieves;
(2) add 8g germanium dioxide (GeO2) in 50ml distilled water, fully after the dissolving, transferring the pH value of germanium solution with sodium hydroxide is 5.0;
(3) according to the 100ml germanium solution: the ratio of 1000g dry yeast is added into dry yeast after step (1) is sieved with germanium solution;
(4) dry yeast that step (3) has been added germanium solution carries out expandedly with the quick-fried machine of extruding spray, and expanded condition is:
Swelling temperature: 125 ℃
Input speed: 200g/min
Screw speed: 15Hz
Rate of water make-up: 5g/100g dry yeast
(5) yeast after the puffing washs three times with pure water, the centrifugal 15min of 3500r/min;
(6) washing get after centrifugal be deposited in 50 ℃ down oven dry 24h promptly get germanium-enriched yeast.
The dry yeast sporoderm-broken rate is 75%; Germanium-enriched yeast organic germanium amount is 1310 μ g/g dry yeast.
Embodiment 3
Concrete operations step of the present invention is as follows:
(1) gets beer waste yeast, cross the 80 mesh sieve removal of impurity; (look yeast select for use other purposes sieve also can);
(2) add 16g germanium dioxide (GeO2) in 50ml distilled water, fully after the dissolving, transferring the pH value of germanium solution with sodium hydroxide is 7.0;
(3) according to the 60ml germanium solution: the ratio of 1000g dry yeast is added into dry yeast after step (1) is sieved with germanium solution;
(4) dry yeast that step (3) has been added germanium solution carries out expandedly with the quick-fried machine SPJ-40 of extruding spray, and expanded condition is:
Swelling temperature: 140 ℃
Input speed: 300g/min
Screw speed: 30Hz
Rate of water make-up: 80g/100g dry yeast
(5) yeast after the puffing washs three times with pure water, the centrifugal 15min of 3500r/min;
(6) washing get after centrifugal be deposited in 50 ℃ down dry 24h promptly get germanium-enriched yeast.
The dry yeast sporoderm-broken rate is 70%; Germanium-enriched yeast organic germanium amount 1180 μ g/g dry yeast.
The yeast organic germanium content height that the present invention produces, rich germanium is effective, thoroughly solves because of the difficult difficult problem that can't industrialization processing of broken wall.A complete set of technology is simple, easy to operate, continuity is good, treatment capacity is big, easily realizes industrialization, and gained germanium-enriched yeast steady quality, safety are constant substantially.
The inventive method can apply in the comprehensive utilization of beer waste yeast simplely, turns waste into wealth, and reduces environmental pollution, has increased value-added content of product, has improved the economic benefit of beer enterprise.Therefore, no matter consider, all have great importance and be worth from economy, environmental protection, technology each side.
Claims (3)
1. the preparation method of a germanium-enriched yeast is characterized in that it being dry yeast to be added inorganic Ge solution and yeast content chelating prepares described germanium-enriched yeast in extruding puffing broken wall treatment process; Described inorganic Ge is a germanium dioxide;
The preparation method of described germanium-enriched yeast may further comprise the steps:
(1) the dry yeast removal of impurity of sieving;
(2) add inorganic Ge solution;
(3) extruding puffing;
(4) washing removes inorganic Ge;
(5) be drying to obtain germanium-enriched yeast;
Wherein, the compound method of the described germanium solution of step (2) is with 8～16g germanium dioxide: the ratio of 50ml distilled water adds in the distilled water germanium dioxide fully that dissolving back adjust pH is 5.0～7.0;
The described germanium solution of step (2) is according to 30～200ml germanium solution: the ratio of 1000g dry yeast be added into the dry yeast of handling through step (1);
The described expanded condition of step (3) is: adopt the quick-fried machine of extruding spray, swelling temperature: 125 ℃～140 ℃; Input speed: 200～300g/min; Screw speed: 15～30Hz; Rate of water make-up: 5～80g/100g dry yeast;
It is with the centrifugal 15min of 3500r/min behind the yeast of the expanded back of pure water washing that the described washing of step (4) removes inorganic Ge;
The described drying of step (5) is dry 24h under 50 ℃ of conditions of temperature.
2. according to the preparation method of the described germanium-enriched yeast of claim 1, it is characterized in that described dry yeast is industrial waste yeast.
3. the preparation method's of the described germanium-enriched yeast of claim 1 application is characterized in that being applied to utilize beer waste yeast to produce germanium-enriched yeast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101942127A CN101709273B (en) | 2009-11-27 | 2009-11-27 | Germanium-enriched yeast and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101942127A CN101709273B (en) | 2009-11-27 | 2009-11-27 | Germanium-enriched yeast and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101709273A CN101709273A (en) | 2010-05-19 |
| CN101709273B true CN101709273B (en) | 2011-11-16 |
Family
ID=42402079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101942127A Expired - Fee Related CN101709273B (en) | 2009-11-27 | 2009-11-27 | Germanium-enriched yeast and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101709273B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105602861A (en) * | 2015-10-20 | 2016-05-25 | 珲春市龙裕农业发展集团有限公司 | Germanium-rich yeast additive adopting rice bran as medium, and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177301A (en) * | 1995-11-08 | 1998-03-25 | 维瓦美国销售有限公司 | Germanium yeast human diet nutritional supplement |
| CN101112400A (en) * | 2007-07-25 | 2008-01-30 | 刘士峰 | oral liquid and capsule for preventing and curing various diseases |
-
2009
- 2009-11-27 CN CN2009101942127A patent/CN101709273B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177301A (en) * | 1995-11-08 | 1998-03-25 | 维瓦美国销售有限公司 | Germanium yeast human diet nutritional supplement |
| CN101112400A (en) * | 2007-07-25 | 2008-01-30 | 刘士峰 | oral liquid and capsule for preventing and curing various diseases |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101709273A (en) | 2010-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107213872B (en) | A kind of preparation method of the hydro-thermal biomass carbon material of efficient absorption copper ion load calcium carbonate | |
| CN101822385B (en) | Method for extruding, puffing and modifying dietary fibers through blast action of carbon dioxide | |
| CN104987142A (en) | Process for producing solid organic fertilizer by using kitchen waste | |
| CN102441555B (en) | Comprehensive utilization and disposal method of urban domestic garbage | |
| CN103755975B (en) | A kind of wood fibre esterification modification prepares the method for bio-based plastics | |
| CN104839437A (en) | Method for producing feed from waste vegetable leaf juice | |
| CN103088692A (en) | Method for selectively separating lignin and cellulose from lignocellulose biomasses | |
| CN105505998B (en) | A kind of method that cascade utilization Chinese medicine slag prepares ethyl alcohol and charcoal | |
| CN108409813A (en) | A method of continuously extracting flaxseed gum and flax lignan from flaxseed meal | |
| CN103130913B (en) | A kind of calcium pectinate and production method thereof and application | |
| CN102733224A (en) | Method for separating corncob cellulose by using ionic liquid | |
| CN104130034A (en) | Method for producing fiber board and organic fertilizer by utilizing garden waste | |
| CN106086106A (en) | A kind of many hydrogen bond donors eutectic solvent pretreatment corn straw and circulation utilization method thereof | |
| CN102766703B (en) | Hydrolysis method for hemicelluloses of lignocelluloses | |
| CN103111265A (en) | Preparation method of adsorbent for removing antibiotics in water, prepared adsorbent and application | |
| CN102994564A (en) | Method for preparing biogas by using peat as raw material | |
| CN102826548A (en) | Banana tree base activated carbon and preparation method thereof | |
| CN105296329A (en) | Device and method for performing continuous pretreatment on lignocellulose material by utilizing microwave-diluted acid steam explosion | |
| CN101709273B (en) | Germanium-enriched yeast and preparation method thereof | |
| Ren et al. | Waste mycelium processing and resource utilization | |
| CN101709274A (en) | Selenium-enriched yeast and preparation method thereof | |
| CN103907938A (en) | Preparation method of jujube fruit residue dietary fiber | |
| CN101258922A (en) | Comprehensive utilization aloe skin processing method | |
| CN103409195B (en) | The production method of the shaping granular fuel of a kind of cold conditions extruding classification | |
| CN106244279A (en) | The method preparing biomass fuel for primary raw material with fresh cattle manure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111116 Termination date: 20141127 |
|
| EXPY | Termination of patent right or utility model |