CN101704904B - Preparation method and application of kudzu polysaccharide extract - Google Patents
Preparation method and application of kudzu polysaccharide extract Download PDFInfo
- Publication number
- CN101704904B CN101704904B CN2009102350780A CN200910235078A CN101704904B CN 101704904 B CN101704904 B CN 101704904B CN 2009102350780 A CN2009102350780 A CN 2009102350780A CN 200910235078 A CN200910235078 A CN 200910235078A CN 101704904 B CN101704904 B CN 101704904B
- Authority
- CN
- China
- Prior art keywords
- kudzu
- polysaccharide
- extract
- polysaccharide extract
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention relates to a kudzu polysaccharide extract as well as a preparation method and application thereof. The method comprises the following steps of: A, smashing kudzu: smashing kudzu to 60-150 meshes; B, extracting polysaccharide: extracting with water of 8-20 times based on weight, carrying out circumfluence extraction for 2-4 hours at 60-100 DEG C; C, centrifugalizing extracting solution wherein the centrifugal speed is 3000-4000r/min; D, taking supernatant of centrifugalized solution to concentrate to a certain concentration with the concentration time of 8-20 times; adding ethanol for deposition ,wherein the concentration of ethanol in concentrated solution of polysaccharide is 60-85%, then centrifugalizing or filtering to remove undissolved substance; E, carrying out secondary ethanol precipitation on the supernatant with the conditions being to the same as that of the step D, then drying to obtain the kudzu polysaccharide extract. The polysaccharide extract is dried for 16-30 hours by using a freeze-dry method. The method for preparing kudzu polysaccharide extract is simple and convenient and oxidation resisting functional foods can be prepared by using the kudzu polysaccharide extract; meanwhile, the invention makes waste profitable, improves the additional value of kudzu byproduct and improves the rate of multipurpose utilization of kudzu resource.
Description
Technical field
The present invention relates to a kind of kudzu polysaccharide extract, refer in particular to kudzu polysaccharide extract, belong to the health food technology field with antioxygenation.
Background technology
Elegant jessamine is the perennial defoliation liana of pulse family, has medicine-food two-purpose concurrently and is worth.The root of kudzu vine that Chinese Pharmacopoeia records is the dry root of elegant jessamine, and it is very wide to distribute in China, and except that Tibet and Xinjiang, all there is distribution all the other each provinces and regions, its medicinal Shennong's Herbal that is recorded in the earliest.The root of kudzu vine contains the various active composition; Be main wherein with osajin compositions such as puerarin, daidzin and daidzeins; Puerarin can be used for treating diseases such as cardiovascular disorder, stenocardia and heart disorder, and daidzin and daidzein can be used for treatment and prevention female diseases.The research and development of China elegant jessamine at present all concentrates on the root of kudzu vine and FI puerariae position, and few to the research of kudzu, and the research of kudzu is primarily aimed at the osajin composition, and is few to other activeconstituents such as polysaccharide and bioactive research.Kudzu is kudzu stem and Ge Ye, is agricultural wastes, does not also obtain development and use at present.Kudzu contains the various active composition, mainly comprises flavonoid, polysaccharide, volatile oil, VITAMINs and mineral substance etc.About the existing patent report of the research of kudzu NOVASOY 400.Chinese patent CN1769278A discloses " method of extraction separation NOVASOY 400 from kudzu " on May 10th, 2006, and isolating extractive of general flavone is puerarin and daidzein from kudzu.Chinese patent CN1723989A discloses " kudzu extract, Preparation method and use " on January 25th, 2006, and this extract main component is the kudzu total flavones, is used to treat the climacteric syndrome medicine.Chinese patent CN1911923A discloses the method for separating soybean aglucone " a kind ofly from kudzu, extract, " on August 23rd, 2007.The inventor adopts reversed phase high efficiency liquid phase method to measure the content of puerarin, daidzin and daidzein in root, stem and the leaf of the Maoshan Mountain, Jiangsu area elegant jessamine simultaneously, and (the RP-HPLC method is measured the content of puerarin, daidzin and daidzein in root, stem and the leaf of elegant jessamine simultaneously; Food science; 2009,30 (14): 248-252), the result shows; Puerarin, daidzin and daidzein content are all far below the content in the root of kudzu vine in the kudzu, and the value of exploiting and utilizing of NOVASOY 400 is low in the visible kudzu.Polysaccharide is one of four big base substances that constitute life, and multiple biological activity is arranged.But relevant kudzu polysaccharide and its production and use do not see domestic and international report so far, do not see that corresponding patent is open yet.
Summary of the invention
The objective of the invention is provides a kind of kudzu polysaccharide extract in order to overcome above disadvantages of background technology.
With the polyoses extract of the present invention's preparation, polysaccharide content is greater than 30%, and yield is between 7.0～10%.
Preparing polyoses extract of the present invention comprises the steps:
A, kudzu are pulverized; Wherein kudzu is crushed to 60～150 orders.
B, polysaccharide extract; With the water extraction of 8～20 times of amounts, extract 2～4h 60～100 ℃ temperature refluxed, extract 2～4 times.
C, extracting solution are centrifugal; Centrifugal speed is 3000～4000r/min.
D, centrifugal back liquid are got supernatant concentration to finite concentration, and spissated multiple is 8～20 times; Add ethanol sedimentation, make that alcohol concn is 60～85% in the liquid concentrator of polysaccharide, centrifugal again or filtration method is removed insolubles;
E, supernatant carry out the secondary ethanol sedimentation, and condition is with step D, and is dry again, gets kudzu polysaccharide extract.Polyoses extract adopts freeze-drying, dry 16～30h.
Kudzu polysaccharide content is measured with the phenolsulfuric acid method.
Adopt and remove hydroxyl radical free radical, superoxide radical test investigation kudzu polysaccharide anti-oxidant activity.The result shows that kudzu polysaccharide has good anti-oxidant activity, and therefore polyoses extract of the present invention can be used for preparing oxidation resistant functional foodstuff.
Advantage of the present invention: kudzu polysaccharide extract preparation method of the present invention is easy, can be prepared into oxidation resistant functional foodstuff; This invention simultaneously can be turned waste into wealth, and promotes the added value of root of kudzu vine sub product, improves the comprehensive utilization ratio of root of kudzu vine resource.
Embodiment
In order to be illustrated more clearly in content of the present invention, with the specific embodiment explanation as follows, specific embodiment does not limit context of the present invention.
The preparation of kudzu polysaccharide
Embodiment 1
Get kudzu sample 100g, kudzu is crushed to 60 orders, with the water of 8 times of amounts in 60 ℃ of refluxing extraction 2 times, each 2 hours; Centrifugal with 3000r/min, the water extract is concentrated into 100mL, and liquid concentrator is with 100mL60% ethanol alcohol precipitation; Hold over night, suction filtration, supernatant carries out the secondary alcohol precipitation; Lyophilize 16h obtains kudzu polysaccharide extract 7.23g, and its polysaccharide content is 30.29%, and yield is 7.23%.
Embodiment 2
Get kudzu sample 100g, kudzu is crushed to 100 orders, with the water of 15 times of amounts in 80 ℃ of refluxing extraction 3 times, each 3 hours; Centrifugal with 3600r/min, the water extract is concentrated into 100mL, and liquid concentrator is with 100mL75% ethanol alcohol precipitation; Hold over night, centrifugal with 3600r/min, supernatant carries out the secondary alcohol precipitation; Lyophilize 24h obtains kudzu polysaccharide extract 8.39g, and its polysaccharide content is 30.27%, and mass yield is 8.39%.
Embodiment 3
Get kudzu sample 100g, kudzu is crushed to 150 orders, with the boiling water refluxing extraction of 20 times of amounts 4 times, and each 4 hours; 4000r/min is centrifugal, and the water extract is concentrated into 100mL, and liquid concentrator is with 100mL85% ethanol alcohol precipitation; Hold over night, suction filtration, supernatant carries out the secondary alcohol precipitation; Lyophilize 30h obtains kudzu polysaccharide extract 9.47g, and its polysaccharide content is 30.31%, and yield is 9.47%.
The test of kudzu polysaccharide antioxygenic activity
Kudzu polysaccharide extract is removed the hydroxyl radical free radical effect
Through removing the anti-oxidant activity that given the test agent is investigated in the hydroxyl radical free radical test.
1, experiment material
1.1 medicine and reagent
Kudzu polysaccharide extract: lot number 081021, the aqueous solution that is mixed with crude drug concentration and is 0.5mg/mL is subsequent use;
Phenanthroline solution: the ethanol solution that is mixed with 0.75mmol/L;
Phosphate buffer soln: the buffered soln that is mixed with 0.2mol/L pH7.40;
Copperas solution: the ethanol solution that is mixed with 0.75mmol/L;
Ydrogen peroxide 50: be mixed with 0.01% ydrogen peroxide 50.
1.2 instrument
UV2450 ultraviolet spectrophotometer: day island proper Tianjin company;
Electronic balance: Beijing Sai Duolisi instrument system ltd;
Rotary Evaporators RE-52C: Yuhua Instrument Co., Ltd., Gongyi City;
Freeze drier FD-1C-50: Beijing rich doctor health laboratory apparatus ltd.
2, experimental technique
Get 1mL phenanthroline solution in test tube; Add 2mL phosphate buffer soln and 1mL zero(ppm) water successively, fully behind the mixing, add 1mL copperas solution mixing; Add the 1mL ydrogen peroxide 50 again; In 37 ℃ of water-bath 60min, survey its light absorption value at 536nm, measured data are the light absorption value (A (damage)) of damage pipe; Replace 1mL zero(ppm) water then to record the light absorption value (A (appearance)) of sample with the 1mL sample liquid; The light absorption value that uses 1mL zero(ppm) water to replace the 1mL ydrogen peroxide 50 to record is A (not).Triplicate.Clearance rate is calculated as follows:
Clearance rate S (%)=(A (appearance)-A (damage))/(A (not)-A (damage)) * 100%
3, experimental result
Fig. 1 is kudzu polysaccharide extract and V
CTo the removing effect of hydroxyl radical free radical, visible, the removing of polyoses extract is effective, and when concentration was 1mg/mL, clearance rate was 74.85%, its IC
50Value is removed ability and the Vc (IC of OH for 0.7511mg/mL, kudzu polysaccharide extract
50Be 0.3477mg/mL) close.
4, conclusion
Kudzu polysaccharide extract has the effect of good removing hydroxyl radical free radical, and it removes effect and V
CClose.
The removing superoxide radical effect of embodiment 5, kudzu polysaccharide extract
Through removing the anti-oxidant activity that given the test agent is investigated in the superoxide radical test.
1, experiment material
1.1 medicine and reagent
Kudzu polysaccharide extract: lot number 081021, the aqueous solution that is mixed with crude drug concentration and is 0.5mg/mL is subsequent use;
Phenanthroline solution: the ethanol solution that is mixed with 0.75mmol/L;
Phosphate buffer soln: the buffered soln that is mixed with 0.01M pH8.2;
Pyrogallol solution: the aqueous solution that is mixed with 45mM;
Hydrochloric acid: the hydrochloric acid soln that is mixed with 10M.
1.2 instrument
UV2450 ultraviolet spectrophotometer: day island proper Tianjin company;
Electronic balance: Beijing Sai Duolisi instrument system ltd;
Rotary Evaporators RE-52C: Yuhua Instrument Co., Ltd., Gongyi City;
Freeze drier FD-1C-50: Beijing rich doctor health laboratory apparatus ltd.
2, experimental technique
Get the 9mL phosphate buffered saline buffer and mix, 25 ℃ of water bath with thermostatic control 15min with the 1mL sample liquid.Get the 3mL mixed solution in cuvette, add the 0.1mL pyrogallol, shake up, added 1 termination reaction of hydrochloric acid, working sample light absorption value (A at the 4th minute in the 325nm place
1), mensuration does not add the sample liquid of pyrogallol and the light absorption value (A of phosphoric acid salt mixed solution respectively simultaneously
2) and with the phosphoric acid salt of 1mL solvent replacement sample liquid and the light absorption value (A of pyrogallol mixed solution
3), triplicate.O
2-Clearance rate is calculated as follows:
Clearance rate S (%)=[1-(A
1-A
2)/A
3] * 100%
3, experimental result
Fig. 2 is kudzu polysaccharide extract and V
CTo the removing effect of superoxide radical, visible, polyoses extract its clearance rate when 1mg/mL reaches 63.95%, its IC
50Value is the 0.9229mg/mL (IC of Vc
50Be 0.2299mg/mL).It is thus clear that it is good that polyoses extract is removed the superoxide radical activity.
4, conclusion
It is good that kudzu polysaccharide extract is removed the superoxide radical activity.
Claims (4)
1. the preparation method of kudzu polysaccharide extract is characterized in that carrying out according to following step:
Get kudzu sample 100g, kudzu is crushed to 60 orders, with the water of 8 times of amounts in 60 ℃ of refluxing extraction 2 times, each 2 hours; Centrifugal with 3000r/min, the water extract is concentrated into 100mL, and liquid concentrator is with 100mL60% ethanol alcohol precipitation; Hold over night, suction filtration, supernatant carries out the secondary alcohol precipitation; Lyophilize 16h obtains kudzu polysaccharide extract 7.23g, and its polysaccharide content is 30.29%, and yield is 7.23%.
2. the preparation method of kudzu polysaccharide extract is characterized in that carrying out according to following step:
Get kudzu sample 100g, kudzu is crushed to 100 orders, with the water of 15 times of amounts in 80 ℃ of refluxing extraction 3 times, each 3 hours; Centrifugal with 3600r/min, the water extract is concentrated into 100mL, and liquid concentrator is with 100mL75% ethanol alcohol precipitation; Hold over night, centrifugal with 3600r/min, supernatant carries out the secondary alcohol precipitation; Lyophilize 24h obtains kudzu polysaccharide extract 8.39g, and its polysaccharide content is 30.27%, and mass yield is 8.39%.
3. the preparation method of kudzu polysaccharide extract is characterized in that carrying out according to following step:
Get kudzu sample 100g, kudzu is crushed to 150 orders, with the boiling water refluxing extraction of 20 times of amounts 4 times, and each 4 hours; 4000r/min is centrifugal, and the water extract is concentrated into 100mL, and liquid concentrator is with 100mL85% ethanol alcohol precipitation; Hold over night, suction filtration, supernatant carries out the secondary alcohol precipitation; Lyophilize 30h obtains kudzu polysaccharide extract 9.47g, and its polysaccharide content is 30.31%, and yield is 9.47%.
4. the application of kudzu polysaccharide extract in the oxidation resistant functional foodstuff of preparation for preparing according to claim 1,2 or 3 described methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102350780A CN101704904B (en) | 2009-11-13 | 2009-11-13 | Preparation method and application of kudzu polysaccharide extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102350780A CN101704904B (en) | 2009-11-13 | 2009-11-13 | Preparation method and application of kudzu polysaccharide extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101704904A CN101704904A (en) | 2010-05-12 |
| CN101704904B true CN101704904B (en) | 2012-05-23 |
Family
ID=42375162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102350780A Expired - Fee Related CN101704904B (en) | 2009-11-13 | 2009-11-13 | Preparation method and application of kudzu polysaccharide extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101704904B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102241786B (en) * | 2011-06-07 | 2013-04-17 | 江苏大学 | Preparation method and application of selenium enriched puerarin polysaccharide |
| CN102603914B (en) * | 2012-04-20 | 2014-01-15 | 河南中医学院 | Preparation method of Caulis Lonicerae polysaccharide |
| CN103111605B (en) * | 2013-03-04 | 2014-03-19 | 宁波海湖蓄电池有限公司 | Cast-welding machine using storage batteries |
| CN109303794B (en) * | 2017-07-27 | 2021-08-06 | 恩施硒德生物工程有限公司 | Selenium-rich cardamine violifolia extract and application thereof in liver protection |
| CN111004336B (en) | 2020-03-09 | 2020-06-12 | 江西中医药大学 | Kudzu root polysaccharide and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493695A (en) * | 2003-08-22 | 2004-05-05 | 李全宏 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
| CN1723989A (en) * | 2005-07-08 | 2006-01-25 | 山东省中医药研究院 | Extractive of kudzu, and its prepn. method and use |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
-
2009
- 2009-11-13 CN CN2009102350780A patent/CN101704904B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493695A (en) * | 2003-08-22 | 2004-05-05 | 李全宏 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
| CN1723989A (en) * | 2005-07-08 | 2006-01-25 | 山东省中医药研究院 | Extractive of kudzu, and its prepn. method and use |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101704904A (en) | 2010-05-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1066055C (en) | Production method for extracting flavonoid compound extract or powder from bamboo leaf | |
| CN102241786B (en) | Preparation method and application of selenium enriched puerarin polysaccharide | |
| CN101704904B (en) | Preparation method and application of kudzu polysaccharide extract | |
| CN102302553B (en) | Extraction method of key components of Chinese magnoliavine fruit medicament | |
| CN103735633B (en) | A kind of preparation method of instant gardenia powder | |
| CN101683360A (en) | Extraction and purifying technology of propolis flavones and anticancer mechanism thereof | |
| CN101781279B (en) | Preparation method of grape seed procyanidin | |
| CN101401830B (en) | Preparation method for extracting flavone from purslane and uses thereof | |
| CN104000892A (en) | Method for extracting peony general flavone from peonies | |
| CN101475459A (en) | Method for extracting pinostrobin and beta-sitosterin from hickory nut epicarp | |
| CN112656707A (en) | Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application thereof | |
| CN110496179A (en) | A kind of method and application for rapidly and efficiently extracting antioxidant in Areca | |
| CN108329368A (en) | A method of preparing scutelloside from radix scutellariae | |
| CN101773553A (en) | Semiaquilegia root extract, preparation method and application thereof | |
| CN102988457A (en) | Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof | |
| CN101361786A (en) | Total-flavone extraction and purification and its monomer separation method from Lespedaza bedysaroides | |
| CN102464683A (en) | Method for extracting oleuropein from olive leaves | |
| CN105601707B (en) | A kind of anti-oxidant tetrapeptide of dandelion seed and preparation method thereof | |
| CN102000127A (en) | Preparation method of chimonanthus inhibited acetylcholinesterase active part and application thereof | |
| CN106883306A (en) | A kind of extraction separation method of the sunflower stem core polysaccharide with antitumor activity | |
| EP2207559A2 (en) | Wound-healing agent containing momordicae semen extract | |
| CN113925081A (en) | Natural plant anticorrosion composition, preparation method and application thereof | |
| CN101380339A (en) | Extraction method of Heidouba effective constituent | |
| CN108969580B (en) | Preparation method and application of blue cloth total tannin | |
| CN102670935A (en) | Method for extracting total saponins from allium chinense |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20151113 |
|
| EXPY | Termination of patent right or utility model |