CN101703809A - Preparation method of porous collagen/polylactic acid bracket - Google Patents
Preparation method of porous collagen/polylactic acid bracket Download PDFInfo
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- CN101703809A CN101703809A CN200910224041A CN200910224041A CN101703809A CN 101703809 A CN101703809 A CN 101703809A CN 200910224041 A CN200910224041 A CN 200910224041A CN 200910224041 A CN200910224041 A CN 200910224041A CN 101703809 A CN101703809 A CN 101703809A
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- polylactic acid
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- porous collagen
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Abstract
The invention discloses a preparation method of a porous collagen/polylactic acid bracket, comprising the following steps: a. dissolving the collagen protein in acetone solution with the concentration of 0.05mol/L to prepare collagen protein solution; b. dissolving the polylactic acid in acetone solution with the concentration of 0.05mol/L to prepare polylactic acid solution; c. mixing the collagen protein solution and the polylactic acid solution according to the weight fraction of 6:4; d. adding epidermal cell growth factor (EGF) in the mixed solution; and e. reducing the temperature of the mixed solution from 25 DEG C to minus 30 DEG C to minus 70 DEG C by controlling the rate of temperature reduction, and freeze-drying for 12 hours, thus obtaining the porous collagen/polylactic acid bracket with the porosity of 85 percent.
Description
Technical field
The present invention designs a kind of preparation method that can be used as the porous collagen/polylactic acid bracket of medical material.
Background technology
The topmost composition of porous collagen/polylactic acid bracket is a collagen protein, similar to the human collagen protein structure, be well suited for the reparation and the regeneration of human organ, can promote the growth of wound healing and granulation tissue, have good hemostasis and packing action, can be used for promoting wound healing, hemostasis, the filling of operation residual cavity etc.Polylactic acid is a kind of bioabsorbable polymer material with good biocompatibility, has certain mechanical strength and degradable performance, and its degradation rate can be adjusted, and can keep certain intravital degradable speed.Epidermal growth factor (EGF) is that last skin system cell is had the powerful peptide that promotes proliferation function, is the polypeptide of 53 amino acid residues, and the propagation that fibroblast, vascular smooth muscle cell, hepatocyte and digestive tract mucosa cell etc. is gone up skin system cell has inducing action.Support should have suitable average pore size, guarantees suitable cell adhesion area when cell is moved between the hole.Therefore, porous collagen/polylactic acid bracket had both had excellent biological compatibility and anthemorrhagic performance, and scalable degradation rate and on cell proliferation have inducing action again, was a kind of more satisfactory bio-medical material.
Freeze-drying is one of common method of preparation porous support materials, adopts the mixed solution of this method collagen, polylactic acid and somatomedin at first to be frozen, and forms the mixture of collagen, polylactic acid, somatomedin and ice crystal thereof along with decrease of temperature.In dry run, the final porous support that forms of ice crystal distillation.Quick, the out-of-control quenching program that adopts in freeze-drying process can cause the conduction of heat situation bigger with spatial variations in time, and makes the nucleation of ice crystal and growth become inhomogeneous, finally causes the variation of supporting structure.Therefore in preparation process, it also is very important controlling cryodesiccated freezing rate.
By retrieval, do not see the report of this preparation method and material thereof before this.
Summary of the invention
The invention provides a kind of preparation method that can be used as the porous collagen/polylactic acid bracket of medical material.And by the freeze drying process parameter control being obtained the high porous collagen/polylactic acid bracket of the even porosity in aperture.
The following technical scheme of the concrete employing of the present invention:
A kind of preparation method of porous collagen/polylactic acid bracket comprises the following steps:
A. collagen protein is dissolved in the acetone soln that concentration is 0.05mol/L and makes collagen solution; B. polylactic acid is dissolved in the acetone soln that concentration is 0.05mol/L and makes polylactic acid solution; C. with collagen solution and polylactic acid solution 6: 4 mixed of mark by weight; D. in mixed solution, add epithelical cell growth factor EGF; E. mixed solution is controlled rate of temperature fall and cooled to-30 ℃ to-70 ℃ from 25 ℃, lyophilization 12 hours, obtaining porosity is porous collagen/polylactic acid bracket material of 85%-90%.
In the preparation method of above-mentioned porous collagen/polylactic acid bracket, epithelical cell growth factor EGF is 0.05mg/ml with respect to the concentration of mixed solution.
The preparation method of above-mentioned porous collagen/polylactic acid bracket, the rate of temperature fall of freezing dry process are 8 ℃/min.
Beneficial effect of the present invention is: the present invention has obtained the uniform collagen/polylactic acid bracket in aperture by the parameter of freeze drying process in porous collagen/polylactic acid bracket preparation process is accurately controlled.
The specific embodiment
Below by embodiment the present invention is described in further detail, a kind of preparation method of porous collagen/polylactic acid bracket comprises the following steps: that a. is dissolved in collagen protein in the acetone soln that concentration is 0.05mol/L to make collagen solution; B. polylactic acid is dissolved in the acetone soln that concentration is 0.05mol/L and makes polylactic acid solution; C. with collagen solution and polylactic acid solution 6: 4 mixed of mark by weight; D. the concentration that adds with respect to mixed solution in mixed solution is the epithelical cell growth factor EGF of 0.05mg/ml; E. mixed solution is controlled rate of temperature fall and cooled to-30 ℃ to-70 ℃ from 25 ℃, lyophilization 12 hours, the rate of temperature fall of freezing dry process is 8 ℃/min, obtains porosity and be porous collagen/polylactic acid bracket material of 90%.
Claims (4)
1. the preparation method of a porous collagen/polylactic acid bracket comprises the following steps: that a. is dissolved in collagen protein in the acetone soln that concentration is 0.05mol/L to make collagen solution; B. polylactic acid is dissolved in the acetone soln that concentration is 0.05mol/L and makes polylactic acid solution; C. with collagen solution and polylactic acid solution 6: 4 mixed of mark by weight; D. in mixed solution, add epithelical cell growth factor EGF; E. mixed solution is controlled rate of temperature fall and cooled to-30 ℃ to-70 ℃ from 25 ℃, lyophilization 12 hours, obtaining porosity is porous collagen/polylactic acid bracket material of 85%-90%.
2. the preparation method of porous collagen/polylactic acid bracket according to claim 1 is characterized in that epithelical cell growth factor EGF is 0.05mg/ml with respect to the concentration of mixed solution.
3. the preparation method of porous collagen/polylactic acid bracket according to claim 1 is characterized in that rate of temperature fall is 8 ℃/min.
4. the preparation method of porous collagen/polylactic acid bracket according to claim 1 is characterized in that porosity is 90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910224041A CN101703809A (en) | 2009-12-01 | 2009-12-01 | Preparation method of porous collagen/polylactic acid bracket |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910224041A CN101703809A (en) | 2009-12-01 | 2009-12-01 | Preparation method of porous collagen/polylactic acid bracket |
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| CN101703809A true CN101703809A (en) | 2010-05-12 |
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| CN200910224041A Pending CN101703809A (en) | 2009-12-01 | 2009-12-01 | Preparation method of porous collagen/polylactic acid bracket |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107376031A (en) * | 2017-08-02 | 2017-11-24 | 北京华信佳音医疗科技发展有限责任公司 | A kind of preparation method of collagen PLA hardened composite |
| CN116617468A (en) * | 2023-05-10 | 2023-08-22 | 万瑞飞鸿(北京)医疗器材有限公司 | Heart stent and preparation method and application thereof |
-
2009
- 2009-12-01 CN CN200910224041A patent/CN101703809A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107376031A (en) * | 2017-08-02 | 2017-11-24 | 北京华信佳音医疗科技发展有限责任公司 | A kind of preparation method of collagen PLA hardened composite |
| CN116617468A (en) * | 2023-05-10 | 2023-08-22 | 万瑞飞鸿(北京)医疗器材有限公司 | Heart stent and preparation method and application thereof |
| CN116617468B (en) * | 2023-05-10 | 2024-05-28 | 万瑞飞鸿(北京)医疗器材有限公司 | Heart stent and preparation method and application thereof |
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Application publication date: 20100512 |