CN101703014B - Sterilization method for squid larva - Google Patents
Sterilization method for squid larva Download PDFInfo
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- CN101703014B CN101703014B CN2009101542209A CN200910154220A CN101703014B CN 101703014 B CN101703014 B CN 101703014B CN 2009101542209 A CN2009101542209 A CN 2009101542209A CN 200910154220 A CN200910154220 A CN 200910154220A CN 101703014 B CN101703014 B CN 101703014B
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- salinity
- water
- thermohaline
- squid larva
- temperature
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a sterilization method for a squid larva, which comprises the steps of (1) temperature and salinity adaptation, namely, putting the squid larva to be sterilized in a sterilization container, and then heating water in the sterilization container from the temperature of water in a culture pond to 26.5 to 30 DEG C, and adjusting the salinity from 32 to 36 thousandths; (2) violent change by fresh water, namely, adding fresh water in water in the sterilization container to lead the salinity of water around the squid larva to be reduced from the salinity in the step of temperature and salinity adaptation to below 10 thousandths in one minute, and damaging the surface structure of germ due to in adaptation to low-salinity water when the gem absorbs water; and (3) temperature and salinity recovery, namely, immediately transferring the squid larva after violent fresh-water change to an environment with temperature of 26.5 to 30 DEG C and salinity of 32 to 36 thousandths for temporary culture, and then recovering the temperature and salinity of water to the standard of water in the culture pond. The sterilization method for the squid larva is simple and easy, causes little damage to the squid larva, has low mortality rate, and is environment-friendly and safe.
Description
Technical field
The present invention relates to a kind of method for disinfection of aquiculture animal, relate in particular to sterilization method for squid larva.
Background technology
Known in the art multiple disinfectant method arranged.The embodiment of these embodiments is based on methods such as chemical disinfection, radiosterilization.But there are several shortcomings in these methods.Chemical disinfection or sterilization are a kind of methods that reaches its limit very soon, can bring the problem of medicament residue, and for example chemical disinfection adopts antibiotic composition, bacterium to become more and more resistance to be arranged and produces drug resistance, and tends to subsidiary aquaculture organism be produced certain toxic action.Yet a small amount of no medicine method for disinfection is arranged also, as Chinese invention patent " activation sterilizing method of activated water and process units thereof ", patent No. ZL02108447.5, a kind of activation sterilizing method and process units thereof of activated water are disclosed, be with of the molecular structure activation of πization platinum ion generator with drinking water, make hydrone be in the high-effect magnetite of airtight tube wall setting and be aided with hyperbaric environment, by most high technology ceramics spheroids with nanoscale hole, become the simplest molecular structure water, and utilize the carbanion that produces in ozone that ozone equipment produces and the reverse osmosis process in and reduction, its carbanion is reduced into carbonic acid gas, and the generation oxidation, expose to the sun according to intensifying oxygen molecule with the UV UV-device again, when making bacterium draw oxygen, destroy the DNA of bacterium by the negative ion of oxygen molecule itself, reach the purpose of sterilization, have the effect of the quality that promotes drinking water.Yet it is unsuitable for being applied in the live body sterilization of aquiculture animal, can cause the death of living animal.
Summary of the invention
The objective of the invention is at prior art provide a kind of simple, little to squid larva injury, lethality is low, the sterilization method for squid larva of Environmental Safety.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: sterilization method for squid larva, it comprises the steps:
(1) thermohaline adapts to: squid larva to be sterilized is moved into disinfecting container, then the water in the disinfecting container is elevated to 26.5 ℃ to 30 ℃ of water temperatures, salinity 32 ‰ to 36 ‰ gradually from the water temperature and the salinity of culturing the pond;
(2) fresh water violent change: the water in the disinfecting container is poured fresh water the water body around the squid larva is reduced to below 10 ‰ within 1 minute from the salinity that thermohaline adapts to step, during the germ suction owing to the incompatibility of low salinity water is destroyed the germ surface structure;
(3) recover thermohaline: will transfer to 26.5 ℃ to 30 ℃ of water temperatures, salinity 32 ‰ to 36 ‰ immediately through the squid larva after the fresh water violent change step process and support temporarily, and return to the water temperature and the salinity of culturing the pond then.
For optimizing technique scheme, the measure of taking also comprises:
Thermohaline adapts in the step, first rising water temperature, and salinity again raises.
Thermohaline adapts in the step, and the rising water temperature adopts gradient increased temperature, and thermograde is not more than 2 ℃; Thermohaline adapts in the step, and the rising salinity adopts gradient to rise salt, and salinity gradient is not more than 5 ‰.
Thermohaline adapts in the step, and the rising salinity is that load weighted sea crystal is placed near the gas stone.
In the fresh water violent change step, adopt squid larva in the disinfecting container and water are together sneaked into the water body of high salinity and reached default salinity.
Recover in the thermohaline step, recover salinity earlier, recover water temperature again.The gradient of recovery temperature and salinity is the inverse operation that thermohaline adapts to corresponding operating in the step in the recovery thermohaline step.
Because the present invention has adopted squid larva to be sterilized is moved into disinfecting container, then the water in the disinfecting container is elevated to 26.5 ℃ to 30 ℃ of water temperatures, salinity 32 ‰ to 36 ‰ gradually from the water temperature and the salinity of culturing the pond; Water in the disinfecting container is poured fresh water the water body around the squid larva is reduced to below 10 ‰ within 1 minute from the salinity that thermohaline adapts to step, during the germ suction owing to the incompatibility of low salinity water is destroyed the germ surface structure; To transfer to 26.5 ℃ to 30 ℃ of water temperatures immediately through the squid larva after the fresh water violent change step process, salinity 32 ‰ to 36 ‰ is supported temporarily, return to steps such as the water temperature of culturing the pond and salinity then, make the germ of squid larva and body surface thereof in the higher water body of water temperature, experience slow dehydration, suction fast, quick this process of dehydration, and complicated experiment by the technical staff, this process control is being injured under the less condition squid larva, and because the germ of squid larva body surface mostly is the seawater fungus strain, very poor to this process short-term regulation, therefore, method of the present invention can only cause under a small amount of dead prerequisite of squid larva, reach the effect that germ is killed, safety and environmental protection, with low cost.
Description of drawings
Fig. 1 is the steps flow chart schematic diagram of the embodiment of the invention.
Embodiment
Below in conjunction with attached embodiment the present invention is described in further detail.
Embodiment: with reference to Fig. 1, sterilization method for squid larva, implementation process is as follows:
In the operating process,, throw something and feed every day and pass through the artemia nauplii of fortification for 2 times the squid larva continuous charge with air pump, the temperature of culturing pool inner water is 19 ℃, and salinity is 17 ‰, and culturing area is located at the Sepiella maindroni procreation ground of the Changjiang river estuary.
(1) thermohaline adapts to: it is the disinfecting container of 10L that squid larva to be sterilized is moved into volume, then the water in the disinfecting container is elevated to 30 ℃ of water temperatures gradually from the water temperature and the salinity of culturing the pond.Heat up to adopt the hot pin mode of heating, and the control water temperature that per hour raises is no more than 1 ℃, avoids because of the water temperature too fast squid larva mortality that causes that raises.After water temperature is elevated to 30 ℃, keep this temperature to begin the salinity that raises after 2 to 3 hours.Every one hour load weighted sea crystal is thrown near the gas stone of inflation, quicken the nature dissolving by seething of bubble, and the injected volume of each sea crystal can make water salinity raise about 4 ‰, enters next procedure after the water salinity in disinfecting container reaches 33 ‰.According to the rule of osmotic pressure, the osmotic pressure of weak solution is directly proportional with the molarity of solution and absolute temperature, that is: osmotic pressure=MRT, and wherein M is a molarity, and R is a gas constant, and T is an absolute temperature.Therefore, in the higher water body of temperature, the variation of molar concentration can cause more violent fluctuation to osmotic pressure.
(2) fresh water violent change: the water in the disinfecting container is poured fresh water the water body around the squid larva is reduced to below 10 ‰ within 1 minute from the salinity that thermohaline adapts to step, during the germ suction owing to the incompatibility of low salinity water is destroyed the germ surface structure.Concrete method of operating is that squid larva and the water through thermohaline adaptation step process in the disinfecting container is together poured in another container that fills 30 ℃ of water temperatures, fresh water volume 30L and the inflation of gas stone.Like this after salt solution and mixing of fresh water, salinity and by mixing moment fresh water to the stimulation of squid larva body surface, can reach the purpose of no medicine sterilization under to the very little situation of squid larva injury about 8.25 ‰.
(3) recover thermohaline: will support temporarily through the water body that the squid larva after the fresh water violent change step process is transferred to 30 ℃ of water temperatures, salinity 33 ‰ immediately, and can draw squid larva by siphonage and carry out transfer operation.Return to the water temperature and the salinity of culturing the pond then, per hour reduce by 4 ‰ salinity by the method that adopts siphon to shift squid larva or in former water body, to add an amount of fresh water, reach 17 ‰ up to water salinity, then per hour reduce temperature and be no more than 1 ℃, reach 19 ℃ up to temperature by the method that adds natural waters 17 ‰ seawater.
Although described the present invention in conjunction with preferred embodiment; right its is not in order to limit the present invention; any those skilled in the art; under the situation that does not break away from the spirit and scope of the present invention; can implement the displacement and the modification of various changes, coordinate to the theme of listing here, so protection scope of the present invention is as the criterion when looking the claim restricted portion that is proposed.
Claims (6)
1. sterilization method for squid larva, it is characterized in that: it comprises the steps:
(1) thermohaline adapts to: squid larva to be sterilized is moved into disinfecting container, then the water in the described disinfecting container is elevated to 26.5 ℃ to 30 ℃ of water temperatures, salinity 32 ‰ to 36 ‰ gradually from the water temperature and the salinity of culturing the pond;
(2) fresh water violent change: will together pour in another container that fresh water is housed through the squid larva and the water of thermohaline adaptation step process in the disinfecting container, water body around the squid larva is reduced to below 10 ‰ within 1 minute from the salinity that thermohaline adapts to step, during the germ suction owing to the incompatibility of low salinity water is destroyed the germ surface structure;
(3) recover thermohaline: will transfer to 26.5 ℃ to 30 ℃ of water temperatures, salinity 32 ‰ to 36 ‰ immediately through the squid larva after the fresh water violent change step process and support temporarily, and return to the water temperature and the salinity of culturing the pond then.
2. sterilization method for squid larva according to claim 1 is characterized in that: described thermohaline adapts in the step, first rising water temperature, and salinity again raises.
3. sterilization method for squid larva according to claim 1 and 2 is characterized in that: described thermohaline adapts in the step, and the rising water temperature adopts gradient increased temperature, and thermograde is not more than 2 ℃; Described thermohaline adapts in the step, and the rising salinity adopts gradient to rise salt, and salinity gradient is not more than 5 ‰.
4. sterilization method for squid larva according to claim 3 is characterized in that: described thermohaline adapts in the step, and described rising salinity is that load weighted sea crystal is placed near the gas stone.
5. sterilization method for squid larva according to claim 4 is characterized in that: in the described recovery thermohaline step, recover salinity earlier, recover water temperature again.
6. sterilization method for squid larva according to claim 5 is characterized in that: in the described recovery thermohaline step, the gradient of recovery temperature and salinity is the inverse operation that described thermohaline adapts to corresponding operating in the step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101542209A CN101703014B (en) | 2009-11-17 | 2009-11-17 | Sterilization method for squid larva |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101542209A CN101703014B (en) | 2009-11-17 | 2009-11-17 | Sterilization method for squid larva |
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| Publication Number | Publication Date |
|---|---|
| CN101703014A CN101703014A (en) | 2010-05-12 |
| CN101703014B true CN101703014B (en) | 2011-08-24 |
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| CN2009101542209A Expired - Fee Related CN101703014B (en) | 2009-11-17 | 2009-11-17 | Sterilization method for squid larva |
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| CN103891648B (en) * | 2014-03-26 | 2015-08-19 | 中国科学院南海海洋研究所 | A kind of light step by step bubble method of defending Hong Kong oyster massive mortality |
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