CN101700276B - Application of medicinal preparation for preparing medicine used for treating chloasma disease - Google Patents
Application of medicinal preparation for preparing medicine used for treating chloasma disease Download PDFInfo
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- CN101700276B CN101700276B CN2009102161666A CN200910216166A CN101700276B CN 101700276 B CN101700276 B CN 101700276B CN 2009102161666 A CN2009102161666 A CN 2009102161666A CN 200910216166 A CN200910216166 A CN 200910216166A CN 101700276 B CN101700276 B CN 101700276B
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- thick paste
- salviae miltiorrhizae
- radix salviae
- ethanol
- chloasma
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Abstract
The invention provides an application of a medicinal preparation for preparing a medicine used for treating a chloasma disease. The medicinal preparation is a thick paste prepared by 835 g of penthorum Chinense pursh and 300 g of the root of red-rooted salvia.
Description
Technical field
The present invention relates to a kind of medical usage of pharmaceutical preparation, belong to food and medicine field, being specifically related to Penthorum chinense and Radix Salviae Miltiorrhizae is the purposes that the pharmaceutical preparation of raw material is used to prepare anti-chloasma medicine.
Background technology
(Chloasma Melasma) is also referred to as chloasma hepaticum to chloasma, is a kind of of facial melanosis, is the pigmentation spots that occurs in face, at present based on the Chinese medicine symptomatic treatment.The generation of chloasma is relevant with liver,spleen,kidney three dirty imbalances, and traditional Chinese medical science typing has caused by hepatic stagnation qi stagnation, controls suitable dispersing the stagnated live-QI to relieve the stagnation of QI, QI and blood regulating; The incoordination between the liver and spleen type is controlled suitable harmonizing the liver and spleen, dissipating phlegm and removing blood stasis; The deficiency of kidney-QI type is controlled suitable invigorating kidney for regulating menstruation, nourshing blood and promoting blood circulation; The not smooth type of blood stasis is controlled suitable blood circulation promoting and blood stasis dispelling, and circulation of qi promoting is soothing the liver.
The granted medicine that is used for chloasma has stonecrop spot removal capsule, menstruation-regulating and speckle-dispelling capsule, HUAYU QUBAN JIAONANG at present, QI and blood and capsule, soothing the liver granule etc., its prescription medication mainly contains Radix Rhodiolae, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Flos Carthami, the Radix Astragali, Radix Rehmanniae Preparata etc., but price is relatively costly.
Penthorum chinense is the dry herb of brave otology plant Penthorum chinense Pursh., and Radix Salviae Miltiorrhizae is the dry root and rhizome of labiate Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bge..The present invention adopts Penthorum chinense and Radix Salviae Miltiorrhizae pharmaceutical formulation to be used for the treatment of chloasma, has not yet to see report, and this pharmaceutical composition can reach blood circulation promoting and blood stasis dispelling through evidence, the effect of soothing liver and strengthening spleen jaundice eliminating, the generation of reduction cell melanocyte.
Summary of the invention
The object of the present invention is to provide the pharmaceutical preparation of a kind of Penthorum chinense and Radix Salviae Miltiorrhizae to be used to prepare the purposes for the treatment of the chloasma disease medicine, the preparation method of described pharmaceutical formulation is as follows:
Get Radix Salviae Miltiorrhizae 300g, add 90% (V/V) alcohol reflux 1.5 hours, filter, filtrate recycling ethanol is to thick paste; Radix Salviae Miltiorrhizae decoction dregs and Penthorum chinense 835g decoct with water 2 times, each 2 hours, collecting decoction filters, and filtrate is condensed into the clear paste that relative density is 1.15～1.18 (60～65 ℃), cooling, add ethanol and make and contain alcohol amount and reach 60%, stir, leave standstill, filter, precipitation merges washing liquid and filtrate with 60% (V/V) washing with alcohol three times, and recovery ethanol also is condensed into the thick paste of relative density 1.30～1.32; Merge above-mentioned two kinds of thick pastes, standby.
Further the thick paste after the above-mentioned merging is added suitable adjuvant as: starch, dextrin, lactose, sucrose, microcrystalline Cellulose, Pulvis Talci, polyvinylpolypyrrolidone, magnesium stearate, coating powder, ethanol, Mel or correctives are prepared as tablet, granule, capsule or pill etc.
The present invention finds that to the influence test of mice whole blood viscosity and melanin generation level the whole blood viscosity of normal mouse has certain reduction effect, can reduce melanic generation after giving chloasma model mice filling stomach by Penthorum chinense and two kinds of extractum of Radix Salviae Miltiorrhizae.
The specific embodiment of form is described in further detail foregoing of the present invention again by the following examples.But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example.All technology that realizes based on foregoing of the present invention all belong to protection scope of the present invention.
The specific embodiment
Embodiment 1 pharmacodynamics test
1.1 summary
Above-mentioned extractum with 2.83,5.67g, 11.34g crude drug/kg irritates stomach and gives the chloasma model mice, administration every day 1 time, successive administration 30 days, mouse orbit is got hematometry whole blood viscosity value after the last administration, gets the PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM that skin of back carries out the dermal melanin cell behind the execution mice.Result of the test shows that this product can obviously reduce chloasma mice whole blood viscosity value, improves blood viscosity, can also reduce the generation of skin melanocyte.
1.2 test objective
Observing Penthorum chinense, Radix Salviae Miltiorrhizae extract irritates stomach and gives behind the chloasma model mice influence to mice whole blood viscosity and melanin generation level.
1.3 test material
1.3.1 be subjected to the reagent thing
Penthorum chinense, Radix Salviae Miltiorrhizae extract (extractum): dark-brown, matter thickness, 5g crude drug/ml.Provide lot number by Chengdu Lisite Pharmaceutical Co., Ltd.: 090601.Face with preceding extractum is for experiment with the medicinal liquid that distilled water is mixed with 0.283g, 0.567g, 1.134g crude drug/ml.
The extractum preparation method: get Radix Salviae Miltiorrhizae 300g, add 90% alcohol reflux 1.5 hours, filter, filtrate recycling ethanol is to thick paste; Medicinal residues and Penthorum chinense 835g decoct with water three times, each 2 hours, collecting decoction filters, and filtrate is condensed into the clear paste that relative density is 1.15～1.18 (60～65 ℃), cooling, add ethanol and make and contain alcohol amount and reach 60%, stir, leave standstill, filter, precipitation merges washing liquid and filtrate with 60% washing with alcohol three times, and recovery ethanol also is condensed into the thick paste of relative density 1.30～1.32; Merge above-mentioned two kinds of thick pastes, standby.
VITAMIN C TABLET derives from Sichuan FuRui Medicine company limited, lot number: 090513.
1.3.2 laboratory animal
Female Kunming mouse, body weight 20 ± 3g is provided by Bill's animal cultivation field, letter city, Jianyang City.
1.3.3 reagent
Progesterone injection: every 10mg/ml, lot number: 090311, Zhejiang Province XianJu Pharmacy stock Co., Ltd.Coomassie brilliant blue, lot number: 20060731.Test kit is Nanjing and builds up bio-engineering research institute.Mouse-anti human monoclonal antibodies HMB45 (melanoma melanoma) instant test kit MAB-0098, the super quick test kit Kit9710 of SP, Fujian steps neoplasm technological development company limited; Concentrated type DAB test kit: ZLI-9032, Bioisystech Co., Ltd of China fir Golden Bridge in Beijing.
1.3.4 instrument
R-80A type fully automatic blood viscosity apparatus (diligent generation Supreme Being's scientific instrument company limited in Beijing), Olympus-BX50 type optical microscope (Japanese Olym2pus), MIAS2000 type computer image analysis system (Sichuan University get the better of software limited company).
1.3.5 method
60 of female mices are divided into 6 groups at random.Normal group is pressed 2ml/kg body weight intramuscular injection sterilized water for injection; All the other 5 groups with progesterone injection in the intramuscular injection of mice back leg, two lower limbs alternately, dosage is 20mg/kg Progesterone (be equivalent to people's clinical equivalent dosage 8 times).Inject 6d weekly, continuously 30d.Modeling begins to gavage medicine or distilled water 0.2ml/10g simultaneously, every day 1 time, 30d continuously.The medicinal liquid that Penthorum chinense, the basic, normal, high dosage of Radix Salviae Miltiorrhizae extractum are respectively 0.283g, 0.567g, 1.134g crude drug/ml (being equivalent to 5,10,20 times of clinical consumptions) is for experiment.Vitamin C dosage 0.1g/kg (be equivalent to the clinical consumption of people 10 times).Behind the last administration 1h, each organizes mouse orbit blood sampling 1ml, contains in containing the adding a cover in the plastic tube of heparin (oneself is dry), measures the whole blood viscosity value.
Strip one of mouse back skin, unhairing, 10% neutral formalin is fixed.Conventional paraffin embedding, the thick section of continuous 5 μ m, HE dyeing.The melanin labelling adopts immunohistochemical method-streptocin biotin method.The one anti-mouse-anti human monoclonal antibodies HMB45 (melano2ma melanoma) that selects for use, the DAB colour developing, haematoxylin is redyed.Replace an anti-negative control of doing with PBS.The positive object-computer image analysis of melanin: each organizes the skin of 8 mices of picked at random, respectively make 1 pathological section, the every visual field of observing 5 non-overlapping copies, after finding positive target, MIAS2000 type computer image analysis system carries out quantitative analysis to target image, draws average area, average optical, the integral optical density of 5 visuals field of every section and every group of positive target of 8 boneblack pigments.
1.4 result
1.4.1 influence to the mice whole blood viscosity
As seen from Table 1, compare with the normal control group, model control group, the different shear rate whole blood viscosity of vitamin C group mice all have obvious rising than normal control group mice.Compare with model control group, medicine group high dose group can obviously reduce the whole blood viscosity value, illustrates that Penthorum chinense, Radix Salviae Miltiorrhizae extractum can obviously improve the hemorheological property of chloasma mice, blood viscosity lowering.
Table 1 Penthorum chinense, Radix Salviae Miltiorrhizae extractum are to the influence of mice whole blood viscosity (x ± s)
Compare * P＜0.05 with model group, * * P＜0.01 (down together)
1.4.2 the PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM of mouse skin melanocyte
HE dyeing: can not find melanocyte under the light microscopic.The HMB45 labelling is observed: visible a small amount of melanocyte in normal group hair follicle, the sebaceous gland basal cell.Model group melanocyte cluster is distributed in hair follicle, the sebaceous gland basal cell, also is dispersed in distribution in epidermal basal cell and the prickle cell layer.Melanocyte only sporadically appears in 4 medication group stratum basale and the hair follicle basal cell.
1.4.3 influence to the mouse skin melanocyte
As seen from Table 2, compare with the normal control group, the positive target area of model control group mouse skin melanocyte, average optical, integral optical density value all have obvious rising than normal control group mice.Compare with model control group, medicine group height, middle dosage group can obviously reduce average optical, the integral optical density value of mouse skin melanocyte positive cell.
Table 2 Penthorum chinense, Radix Salviae Miltiorrhizae extractum are to the influence of mouse skin melanocyte (x ± s)
| Group | Dosage (g/kg) | Mus number (only) | Positive target area (μ m) | Average optical | Integral optical density |
| Normal control | 10 | 2887.56±896.52* | 0.25±0.08** | 847.59±287.15** | |
| The model contrast | 10 | 4231.74±952.33 | 0.39±0.11 | 1445.78±435.56 | |
| The medicine group | 11.34 | 10 | 3560.23±1033.12 | 0.26±0.05* | 887.51±367.52** |
| The medicine group | 5.67 | 10 | 3786.61±1205.87 | 0.28±0.10* | 987.18±321.35* |
| The medicine group | 2.83 | 10 | 3957.42±1125.45 | 0.33±0.07 | 1125.45±412.48 |
| Vitamin C | 0.1 | 10 | 3819.51±1347.81 | 0.34±0.12 | 1189.74±401.58 |
1.5 conclusion
Chloasma is the pigmented dermatosis due to a kind of multifactor, multisystem, the too many levels, and pathogeny complexity, real pathogenic factor are still not fully aware of at present.It increases dermatoses as a kind of pigment, and it too much is its basic reason that melanocyte generates, and the synthetic melanocyte of melanocyte is a very complicated process, is subjected to influence of various factors.Patient with chloasma exists hemodynamic index unusual, and it is in close relations to think that hemorheology changes with chloasma.Be consistent with qi depression to blood stasis, venation block in the Chinese medicine, so blood stasis is one of cause of disease of chloasma.This paper has detected hemorheology index and provides experimental basis for treating this disease.
This experimental studies results shows: Penthorum chinense, Radix Salviae Miltiorrhizae extractum have the effect of soothing liver and strengthening spleen, blood circulation promoting and blood stasis dispelling, can show to reduce chloasma mice whole blood viscosity value, improve blood viscosity, and Penthorum chinense, Radix Salviae Miltiorrhizae extractum can also reduce the generation of skin melanocyte in addition.
1.6 result and discussion
From above-mentioned result of the test as seen: Penthorum chinense, Radix Salviae Miltiorrhizae extractum have certain reduction effect to the whole blood viscosity of normal mouse, can reduce melanic generation, cure mainly analysis from the function of this prescription, it has soothing liver and strengthening spleen, blood circulation promoting and blood stasis dispelling, the effect of treatment chloasma, so we think that the Penthorum chinense, the red sage root composition preparation that adopt this patent preparation have the circulation of the blood of human body of improvement, reduce human body melanocyte level, have the clinical value of treatment chloasma.
The preparation of embodiment 2 thick pastes
Radix Salviae Miltiorrhizae 300g adds 90% alcohol reflux 1.5 hours, filters, and filtrate recycling ethanol is to thick paste; Radix Salviae Miltiorrhizae decoction dregs and Penthorum chinense 835g decoct with water 2 times, each 2 hours, collecting decoction filters, and filtrate is condensed into the clear paste that relative density is 1.15～1.18 (60～65 ℃), cooling, add ethanol and make and contain alcohol amount and reach 60%, stir, leave standstill, filter, precipitation merges washing liquid and filtrate with 60% washing with alcohol three times, and recovery ethanol also is condensed into the thick paste of relative density 1.30～1.32; Merge above-mentioned two kinds of thick pastes, standby.
Embodiment 3 tablets
Get the thick paste cream of embodiment 2, add appropriate amount of starch, dextrin, microcrystalline Cellulose, magnesium stearate etc., mixing, granulate dry, granulate, tabletting, promptly.And can be made into ordinary tablet, dispersible tablet, effervescent tablet according to the difference of adding supplementary product kind.
Embodiment 4 solid particle agent
Get the thick paste of embodiment 2, add appropriate amount of starch, dextrin, cane sugar powder, mixing, it is dry to granulate, granulate, packing, promptly.
Embodiment 5 capsules
Get the thick paste of embodiment 2, add appropriate amount of starch, microcrystalline Cellulose, Pulvis Talci, magnesium stearate etc., mixing breaks into the powder direct packaging behind granulate dry back packing or the extractum convection drying, promptly.
Embodiment 6 pills
Get the thick paste cream of embodiment 2, convection drying is ground into fine powder or adds the appropriate amount of auxiliary materials crushed after being dried and becomes fine powder, adds finite concentration ethanol or the general one-tenth pill of hydromel promptly.
Claims (2)
1. a pharmaceutical preparation is used to prepare the purposes for the treatment of the chloasma disease medicine, and it is characterized in that: described pharmaceutical preparation is the thick paste that is made by Penthorum chinense 835g and Radix Salviae Miltiorrhizae 300g;
The preparation method of described thick paste is: get Radix Salviae Miltiorrhizae 300g, add 90% alcohol reflux 1.5 hours, filter, filtrate recycling ethanol is to thick paste; Radix Salviae Miltiorrhizae decoction dregs and Penthorum chinense 835g decoct with water 2 times, each 2 hours, collecting decoction filters, and it is 1.15～1.18 clear paste at 60～65 ℃ that filtrate is condensed into relative density, cooling, add ethanol and make and contain alcohol amount and reach 60%, stir, leave standstill, filter, precipitation merges washing liquid and filtrate with 60% washing with alcohol three times, and recovery ethanol also is condensed into the thick paste of relative density 1.30～1.32; Merge above-mentioned two kinds of thick pastes, standby.
2. pharmaceutical preparation as claimed in claim 1 is used to prepare the purposes of treatment chloasma disease medicine, it is characterized in that: described thick paste can obtain tablet, granule, capsule or pill through adding starch, dextrin, lactose, sucrose, microcrystalline Cellulose, Pulvis Talci, polyvinylpolypyrrolidone, magnesium stearate, ethanol or Mel.
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| CN102078486B (en) * | 2011-01-21 | 2012-06-20 | 吉林紫鑫药物研究有限公司 | Drug composition for removing chloasma and preparation method thereof |
| CN103315289B (en) * | 2013-05-07 | 2014-10-15 | 江苏丹枫神源生物科技有限公司 | Mono maple pollen extract and application of the same as health care product |
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