CN1016663B - Pig feed - Google Patents

Abstract

Hygromycin, EPIHYGROMYCIN and composition thereof has good inhibition activity to the treponemal propagation of pig dysentery.

Description

Pig feed

The present invention is relevant with use hygromycin, EPIHYGROMYCIN (epihygromycin) or its mixture, to prevent the propagation of pig dysentery treponema (Treponema hyodysenteriae).

Pig dysentery treponema is a kind of large-scale spiral body that causes pig dysentery disease, and it mainly shows is sticking hemorrhagic diarrhea ight soil.Owing to infected in the ight soil of this spirochetal pig a large amount of pig dysentery treponemas is arranged, so environment can very fast infection.It is believed that the swine dysentery that pig dysentery treponema causes in case take place, pig house is extremely difficult to be purified by cleaning.

For the swine rearing person, because the decline of delayed growth that swine dysentery caused and the conversion of material meat is the long-term loss that can't estimate.

For hygromycin, R.C.People such as Pi Teboge are found in streptomyces hygroscopicus (Streptomyces hygroscopicus) culture medium nineteen fifty-three, and have reported its antimicrobial spectrum and infection research result (the 157th page of antibiotic yearbook: 1953-1954) for the first time.Subsequently, nineteen fifty-seven R.L.People such as graceful nanotesla have determined the chemical constitution (79 phases of U.S. chemical institute magazine the 120th page (1957)) of hygromycin.In addition, show that streptomycete (Streptomyces atrofaciens) also produces same substance (the 176th page of United States Patent (USP) the 3rd, 100, on August 6th, 1963).In addition, people such as Sumiki have reported to be produced by the rich streptomycete (Streptomyces noboritoencis) of promise and have examined antibiotic homomycin (the 170th page of antibiotic book series A, 8 phase, (1955)) as the resistive connection of hygromycin congener.And the comparative studies of this kind antibiotic done subsequently by people such as K. Ai Suonuo and hygromycin explanation, two kinds of antibiotic are real to be same substance (the 21st page of 10 phase of antibiotic magazine, (1957)).Recently, K.People such as Ka Jinnuma have reported ST-4331 and hygromycin is a same substance, and have provided its precise structure (the 771st page of 29 phase of antibiotic magazine, (1976)).People such as Wakisaka have then corrected the structure that is proposed, and have provided the existence and the precise structure (the 695th page of 33 phase of antibiotic magazine, (1980)) thereof of EPIHYGROMYCIN.

The present invention provides a composition that contains hygromycin, EPIHYGROMYCIN or its mixture, reaches a mediator or its diluent, and uses above-mentioned substance to prevent the method for pig dysentery treponema propagation.

Hygromycin and EPIHYGROMYCIN not only can be by the method productions that above-mentioned document provided, but also can announce the method that 33-1248 number (1958) are described with Japanese unexamined patent, from using the rich streptomycete of promise to obtain (catalogue is cultivated, the 7th edition (1984) by Osaka fermentation research institute 13065).

Hygromycin, the damp mould of table and composition thereof are high safeties.These materials can mixed state be used or all can be used the oral medication swine dysentery if necessary separately.Pig can be oral or non-enteron aisle take in conjunction with other vectorial these compounds.

When hygromycin and EPIHYGROMYCIN used with its mixed state, its ratio can be selected, but ratio is the hygromycin of the heavy EPIHYGROMYCIN of 0.3-1 part to 1 part of weight preferably, and better ratio is the hygromycin of the heavy EPIHYGROMYCIN of 0.3-0.5 part to 1 part of weight.

In animal produces, normally group feeding.If swine dysentery is to find in swinery, way is the infected individuals of isolating for treatment usually, or uses the foodstuff that contains hygromycin, EPIHYGROMYCIN or its mixture for isolator all animals.The present invention also comprises this isolation or does not use hygromycin, the way of EPIHYGROMYCIN or its mixture.

Composition of the present invention can be by being prepared as follows:

Hygromycin, EPIHYGROMYCIN and composition thereof are selectively with the dilution of liquid or solid diluent, or it is steady for example to be weighed by packing, and make following medicament forms: powdery, fine powder, pill, tablet, liquid, paste, capsule, injection or the like directly or in dilution add in foodstuff or the drinking water after the disperse.Diluent can be any physiology innocuousness material, and preferably a kind of can be as the material or the foodstuff ingredient of foodstuff.The example of described solid diluent comprises: pearling cone meal, wheat flour, oatmeal, corn flour, soy meal, soybean cake, rapeseed cake, rice husk, rice bran, degreasing rice bran, sweet potato flour, mealy potato, soybean milk curd powder, starch, lactose, sucrose, glucose, fructose, yeast, inefficacy yeast, fish meal, talcum powder, sour soil, clay etc.The example of aforesaid liquid diluent comprises the organic solvent of water, physiological saline, tool physiology innocuousness.Also can add suitable assistant in right amount in addition, as emulsifying agent, disperse means, suspending agent, wetting agent, concentrating agents or intensifier, gelatinizing agent, lytic agent or the like.Moreover, also can add anticorrisive agent, fungicide, antiseptic (carbadox, demetridazole or the like), antibiotic (as tiamulin, cillimycin etc.) enzyme preparation and Bacillus acidi lactici agent, and in final composition, replenish vitamin, mineral matter, amino acid and other material selectively.

The present invention cures the composition dosage of swine dysentery and can choose according to following consideration: pig age, condition and drug delivery route etc.For example, when medication in the popular fixed swinery of dysentery, preferable dosage for every day per kilogram use hygromycin, EPIHYGROMYCIN or its mixture 0.05-25 milligram.In this application, add 100/1000000ths 500/10000ths hygromycin, EPIHYGROMYCIN or its mixture in the pig feed, 200/2nds/1000000ths-1,000,000 are better, feed pigs then.For the treatment of infected individuals, preferably dosage for every day per kilogram use hygromycin, EPIHYGROMYCIN or its mixture 0.1-50 milligram.In this uses, in the foodstuff, add 500/2nds/1000000ths-1,000,000 activating agent for well, can add 200/2/1000000ths. five-1,000,000 when special, then to this foodstuff that contains activating agent of sick pig feeding.As for injection, preferable dosage is per kilogram 0.25-25 milligram every day, provides every milliliter of 1-1000 milligram and the activating agent by conventional pharmacology program prescription can be injectable composition, and better dosage then is the 1-500 milligram.Injection composition can be suspension, solution or the emulsion at the oily or the activating agent in the aqueous medium of physiology admissibility, and can comprise following assistant such as suspending agent, stabilizing agent and (or) disperse means, also comprise the anticorrisive agent and the buffer of phenmethylol class if necessary.Composition or reactive compound can be-the reorganization powder, like this, powder just can with suitable medium reorganization, be used for injecting as the sterile distilled water in the dispenser.

Following example will illustrate the further details of invention at present, and present invention need not be confined to this.

Embodiment 1

According to medicament or its premix of treatment swine dysentery of the present invention, can be by hygromycin, EPIHYGROMYCIN or its mixture be made with basic foodstuff or its part component of suitable concentration adding table 1.

The basic foodstuff ingredient (%) of table 1

The characteristic that meat is produced

Material emulsus substitute B

Identify feeding

Corn 20.0 22.0

Jowar 22.0

Pearling cone meal 19.0 22.0

Wheat flour 14.0

Soy meal 4.0

Wheat bran 6.8 12.0

Degreasing rice chaff 4.0 4.0

Fish meal 3.0 4.0

The basic foodstuff ingredient (%) of continuous table 1

The characteristic that meat is produced

Material emulsus substitute B

Identify feeding

Soybean cake 4.0

Skimmed milk powder 9.78

Defatted soybean 5.0

Dehydration whey 5.0

Clover powder 2.5

Glucose 5.0

Food yeast 2.0

Fatty powder 1.5

Calcium carbonate 1.5

Sodium chloride 0.5

Calcium phosphate 0.7 0.8

Asccharin 0.02

Vitamin-mineral matter 0.2 ^(a)0.4 ^(b)

Mixture

DL type dawn propylhomoserin 0.1

Amount to 100.0 100.0

(a) in the per kilogram, the vitamin A of 2,500,000 international unit, the vitamin D of 500,00 international units ₃, the vitamin E of 0.75 gram, the Cobastab of 1 gram ₁Nitrate, the Cobastab of 1.5 grams ₂, the Cobastab of 0.25 gram ₆, 1 milligram Cobastab ₁₂, the calcium pantothenate of 3.5 grams, the niacinamide of 7.5 grams, 50 gram Choline Chlorides, 50 gram iron, 5 gram copper, 25 gram zinc, 15 gram manganese, 0.25 gram cobalt and 0.1 gram iodine.

(b) composition contains mixture A, mixture B, and mixture C, its ratio is 0.15: 0.15: 1.

A mixture: 5% manganese, 5% iron, 1% copper, 6% zinc and 0.1% iodine.

The B mixture: in the gram, the vitamin D of the vitamin A of 10,000 international units and 2,000 international units ₃

The C mixture: in one kilogram, 1 gram Cobastab ₁Nitrate, 7 gram Cobastabs ₂, 0.5 gram Cobastab ₆, 6 gram niacinamides, 10.9 gram calcium pantothenates, and 57.6 gram choline.In addition, add 10 microgram Cobastabs ₁₂

Embodiment 2

In one 25 milliliters bottle, hygromycin, EPIHYGROMYCIN or its mixture of the 4 gram sterile preparation of packing under the aseptic condition, and 180 milligrams of sodium chloride seal subsequently and make injection.When using preparation, inject 20 milliliters of sterile distilled waters, make the solvent that contains every milliliter of 200 milligrams of above-mentioned active components.

Test

Hygromycin, EPIHYGROMYCIN and known anti-swine dysentery preparation-cillimycin, the treponemal antibiotic activity value of anti-pig dysentery sees Table 2.

Table 2

Bacterial strain ⁽¹⁾Minimum antibacterial concentrate (mcg/ml) ^(2-4)

Medicine CD1 DJ70P1 MK2 78/A

Hygromycin 6.25 6.25 6.25 6.25

EPIHYGROMYCIN 12.25 12.5 12.25 12.25

Mixture ⁽⁵⁾6.25 6.25 6.25 6.25

Cillimycin 25 25 25 25

(1) bacterial strain: the pig dysentery treponema bacterial strain that from the sticking hemorrhage diarrhoea ight soil of japanese dysentery pig, separates.

(2) antiseptic authentication method: agar plate dilution.

(3) inoculum size: 10 ⁶CFU/ml-loopful (about 0.5 microlitre).

(4) culture medium condition: anaerobic culture medium was being placed two days in gaspar bottle (BBL) under 37 ℃ of conditions.

(5) mixture: the crystal that contains hygromycin (68%) and EPIHYGROMYCIN (24%).

Claims (1)

1, a kind of pig feed is characterized in that this feed contains hygromycin, EPIHYGROMYCIN or its mixture.