CN101647427A - Equus semen storage diluter and preparing method and use method thereof - Google Patents
Equus semen storage diluter and preparing method and use method thereof Download PDFInfo
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- CN101647427A CN101647427A CN 200910113436 CN200910113436A CN101647427A CN 101647427 A CN101647427 A CN 101647427A CN 200910113436 CN200910113436 CN 200910113436 CN 200910113436 A CN200910113436 A CN 200910113436A CN 101647427 A CN101647427 A CN 101647427A
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Abstract
The invention relates to an equus semen storage diluter and a preparing method and a use method thereof, belonging to the technical field of animal semen storage. The equus semen storage diluter is prepared from double distilled water, glucose, glycin, penicillin, streptomycin and yolk. The equus semen storage diluter has a good effect of preserving the equus semen under the normal or low temperature, has the advantages of simple operation, low cost, easy mastery, and is suitable for popularization and application, which is beneficial to the development of the equus breeding.
Description
Technical field
The present invention relates to the technical field that animal semen is preserved, is that a kind of equus seminal fluid is preserved dilution and production method and using method.
Background technology
Along with present stage is supported donkey industry and horse keeping continuous intensification already by China, test-tube demand is strengthened day by day.Good semen diluent can be preserved seminal fluid for a long time, and is harmless to dam, improves the fertility of conception rate and stallion, donkey.Good semen diluent is test-tube basic assurance and necessary condition.Therefore it is long to develop the holding time, and preservation effect is good, and cost is low, and the effective semen diluent of being impregnated has important economic benefit to artificial insemination.At present both at home and abroad the semen diluent of equus is short to the holding time of sperm viability, and conception rate is low, serious restriction China support donkey industry and horse keeping development already.
Summary of the invention
The invention provides a kind of equus seminal fluid and preserve dilution and production method and using method, it has overcome the deficiency of above-mentioned prior art, and it keeps effectively and has prolonged equus particularly donkey and horse have the activity of the seminal fluid of fertility.
One of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved dilution, and its distilled water by every 70mL to 120mL mixes mutually to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU to 7 grams, glycine 0.2 gram with glucose 4.5 grams and promptly gets equus seminal fluid preservation dilution.
Two of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved dilution, and its distilled water by every 70mL to 120mL mixes mutually to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU, yolk 2mL to 10mL to 7 grams, glycine 0.2 gram with glucose 4.5 grams and promptly gets equus seminal fluid preservation dilution.
Three of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the production method of dilution, it is undertaken by following step: in sterilized container, glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement mixed mutually promptly get the equus seminal fluid and preserve dilution.
Four of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the production method of dilution, it is undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution.
Five of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the production method of dilution, it is undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution.
Six of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the using method of dilution, and it is undertaken by following step: glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement is mixed mutually promptly get equus seminal fluid preservation dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and preserve at normal temperatures or wrap up with the cotton-wool of 0.5 cm thick to be placed on the indoor preservation of refrigerator cold-storage.
Seven of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the using method of dilution, it is undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge and preservation at normal temperatures then.
Eight of technical scheme of the present invention realizes in the following manner: a kind of equus seminal fluid is preserved the using method of dilution, it obtains by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and wraps up with the cotton-wool of 0.5 cm thick and to be placed on the indoor preservation of refrigerator cold-storage.
It is not only very good to equus seminal fluid preservation effects under normal temperature or low temperature such as horse donkeys that equus seminal fluid of the present invention is preserved dilution, and simple to operate, production cost is low, grasp is fit to apply easily, thereby helps the development of equus aquacultures such as horse donkey.
Embodiment
The present invention is not subjected to the restriction of following embodiment, can determine concrete embodiment according to the technical scheme and the actual conditions of the invention described above.
Embodiment 1, and this equus seminal fluid is preserved dilution and mixed mutually to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU to 7 grams, glycine 0.2 gram with glucose 4.5 grams by the distilled water of every 70mL to 120mL and promptly get equus seminal fluid preservation dilution.
Embodiment 2, and this equus seminal fluid is preserved dilution and mixed mutually by the distilled water of every 70mL or 120mL and glucose 4.5 grams or 7 grams, glycine 0.2 gram or 2 grams, penicillin 100,000 IU or 1,000,000 IU, streptomycin 50,000 IU or 500,000 IU and promptly get equus seminal fluid preservation dilution.
Embodiment 3, and this equus seminal fluid is preserved dilution and mixed mutually to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU, yolk 2mL to 10mL to 7 grams, glycine 0.2 gram with glucose 4.5 grams by the distilled water of every 70mL to 120mL and promptly get equus seminal fluid preservation dilution.
Embodiment 4, and this equus seminal fluid is preserved dilution and mixed mutually by the distilled water of every 70mL or 120mL and glucose 4.5 grams or 7 grams, glycine 0.2 gram or 2 grams, penicillin 100,000 IU or 1,000,000 IU, streptomycin 50,000 IU or 500,000 IU, yolk 2mL or 10mL and promptly get equus seminal fluid preservation dilution.
Embodiment 5, the equus seminal fluid of the foregoing description 1 to 4 is preserved the production method of dilution and is undertaken by following step: in sterilized container, glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement mixed mutually promptly get equus seminal fluid preservation dilution.
Embodiment 6, the equus seminal fluid of the foregoing description 1 to 2 is preserved the production method of dilution and undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution.
Embodiment 7, the equus seminal fluid of the foregoing description 3 to 4 is preserved the production method of dilution and undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution.
Embodiment 8, and the equus seminal fluid of the foregoing description 1 to 4 is preserved the production method of dilution and undertaken by following step: glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement is mixed mutually promptly get equus seminal fluid preservation dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and preserve at normal temperatures or wrap up with the cotton-wool of 0.5 cm thick to be placed on the indoor preservation of refrigerator cold-storage.
Embodiment 9, the equus seminal fluid of the foregoing description 1 to 2 is preserved the production method of dilution and undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge and preservation at normal temperatures then.
Embodiment 10, the equus seminal fluid of the foregoing description 1 to 4 is preserved the production method of dilution and undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and wraps up with the cotton-wool of 0.5 cm thick and to be placed on the indoor preservation of refrigerator cold-storage.
In the present invention, yolk is meant the egg yolk liquid of new fresh hen egg.
In the present invention, needed equus fresh semen is meant: the equus fresh semen of motility rate 〉=0.8.
Test example 1, the foregoing description gained equus seminal fluid preservation dilution and other semen diluent low temperature are preserved the influence to the equus sperm viability:
The result of table 1 and table 2 shows, equus is used preceding two kinds of dilutions to the vigor of the sperm weak point of holding time, preserve dilution with equus seminal fluid of the present invention and can obtain good effect as horse, the bright smart dilution of donkey, not only the holding time long, and motility rate height.
Test example 2, the foregoing description gained equus seminal fluid preservation dilution and other semen diluent normal temperature are preserved the influence to the equus sperm viability:
By table 3 and table 4 as can be seen, to preserve the effect that dilution preserves at normal temperature more satisfactory stable for equus seminal fluid of the present invention.
Claims (8)
1, a kind of equus seminal fluid is preserved dilution, it is characterized in that distilled water by every 70mL to 120mL mixes to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU to 7 grams, glycine 0.2 gram mutually with glucose 4.5 grams promptly to get equus seminal fluid preservation dilution.
2, a kind of equus seminal fluid is preserved dilution, it is characterized in that distilled water by every 70mL to 120mL mixes to 2 grams, penicillin 100,000 IU to 100 ten thousand IU, streptomycin 50,000 IU to 50 ten thousand IU, yolk 2mL to 10mL to 7 grams, glycine 0.2 gram mutually with glucose 4.5 grams promptly to get equus seminal fluid preservation dilution.
3, a kind of production method of equus seminal fluid preservation dilution according to claim 1 and 2, it is characterized in that being undertaken: in sterilized container, glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement mixed mutually promptly get the equus seminal fluid and preserve dilution by following step.
4, a kind of production method of equus seminal fluid preservation dilution according to claim 1, it is characterized in that being undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution.
5, a kind of production method of equus seminal fluid preservation dilution according to claim 2, it is characterized in that being undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution.
6, a kind of equus seminal fluid according to claim 1 and 2 is preserved the using method of dilution, it is characterized in that being undertaken by following step: glucose, glycine, penicillin, streptomycin, the yolk of the distilled water of requirement and requirement is mixed mutually promptly get equus seminal fluid preservation dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and preserve at normal temperatures or wrap up with the cotton-wool of 0.5 cm thick to be placed on the indoor preservation of refrigerator cold-storage.
7, a kind of using method of equus seminal fluid preservation dilution according to claim 1, it is characterized in that being undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then adds the penicillin and the streptomycin of requirement and mix in container and just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge and preservation at normal temperatures then.
8, a kind of using method of equus seminal fluid preservation dilution according to claim 2, it is characterized in that being undertaken by following step: glycine and the glucose with requirement places sterilized container earlier, secondly the distilled water mixing that adds requirement in container makes the fully dissolving at normal temperatures of above-mentioned two kinds of materials, and then in container, add the penicillin and the streptomycin of requirement and mix, last in container, add the yolk of requirement again and mix just obtain the equus seminal fluid and preserve dilution; At first above-mentioned equus seminal fluid is preserved dilution and be heated to 37 ℃ with water-bath, secondly the equus fresh semen being carried out volume ratio is 1: 1 to 1: 10 dilution, will dilute good equus fresh semen lucifuge then and wraps up with the cotton-wool of 0.5 cm thick and to be placed on the indoor preservation of refrigerator cold-storage.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200910113436 CN101647427B (en) | 2009-08-26 | 2009-08-26 | Equus semen storage diluter and preparing method and use method thereof |
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| CN 200910113436 CN101647427B (en) | 2009-08-26 | 2009-08-26 | Equus semen storage diluter and preparing method and use method thereof |
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| CN101647427A true CN101647427A (en) | 2010-02-17 |
| CN101647427B CN101647427B (en) | 2012-11-28 |
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Cited By (13)
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| CN102246747A (en) * | 2011-06-23 | 2011-11-23 | 北京市华都峪口禽业有限责任公司 | Semen diluter and preparation method as well as application method thereof |
| CN102578079A (en) * | 2012-02-08 | 2012-07-18 | 青岛德瑞骏发生物科技有限公司 | Base fluid for diluting semens of equus animals and preparation method and use method thereof |
| CN102599148A (en) * | 2012-03-06 | 2012-07-25 | 扬州大学 | Diluent of pigeon semen |
| CN103039433A (en) * | 2012-12-10 | 2013-04-17 | 山东天龙牧业科技有限公司 | Casein anti-freezing agent for cryopreservation of donkey semen and preparation method thereof |
| CN103039434A (en) * | 2012-12-10 | 2013-04-17 | 山东天龙牧业科技有限公司 | Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof |
| CN103120155A (en) * | 2013-02-05 | 2013-05-29 | 新疆维吾尔自治区畜牧科学院畜牧科学研究所 | Cryopreservation solution of horse sperms and freezing method thereof |
| CN103651333A (en) * | 2013-12-23 | 2014-03-26 | 伊犁德瑞骏发生物科技有限公司 | Frozen semen diluent of equus animals and preparation method thereof |
| CN103704202A (en) * | 2013-12-11 | 2014-04-09 | 广西大学 | Pony semen cryopreservation method |
| CN103843758A (en) * | 2014-02-19 | 2014-06-11 | 新疆维吾尔自治区畜牧科学院畜牧研究所 | Low temperature preservation diluent for horse sperm |
| CN110583630A (en) * | 2019-10-22 | 2019-12-20 | 山东畜牧兽医职业学院 | Donkey semen diluent and preparation method thereof |
| CN112155785A (en) * | 2020-10-28 | 2021-01-01 | 内蒙古草原御驴科技牧业有限公司 | Healthy breeding method for improving mating success rate of donkey mothers |
| CN113273566A (en) * | 2021-05-31 | 2021-08-20 | 西北农林科技大学 | Application of glycine as effective component in boar semen normal-temperature preservation solution |
| CN114304139A (en) * | 2022-01-19 | 2022-04-12 | 山东畜牧兽医职业学院 | Semen preserving fluid for improving semen preserving quality and preparation method thereof |
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2009
- 2009-08-26 CN CN 200910113436 patent/CN101647427B/en not_active Expired - Fee Related
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| CN102246747A (en) * | 2011-06-23 | 2011-11-23 | 北京市华都峪口禽业有限责任公司 | Semen diluter and preparation method as well as application method thereof |
| CN102578079A (en) * | 2012-02-08 | 2012-07-18 | 青岛德瑞骏发生物科技有限公司 | Base fluid for diluting semens of equus animals and preparation method and use method thereof |
| CN102599148A (en) * | 2012-03-06 | 2012-07-25 | 扬州大学 | Diluent of pigeon semen |
| CN103039433A (en) * | 2012-12-10 | 2013-04-17 | 山东天龙牧业科技有限公司 | Casein anti-freezing agent for cryopreservation of donkey semen and preparation method thereof |
| CN103039434A (en) * | 2012-12-10 | 2013-04-17 | 山东天龙牧业科技有限公司 | Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof |
| WO2014089924A1 (en) * | 2012-12-10 | 2014-06-19 | 山东天龙牧业科技有限公司 | Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof |
| CN103120155B (en) * | 2013-02-05 | 2014-07-16 | 新疆维吾尔自治区畜牧科学院畜牧科学研究所 | Cryopreservation solution of horse sperms and freezing method thereof |
| CN103120155A (en) * | 2013-02-05 | 2013-05-29 | 新疆维吾尔自治区畜牧科学院畜牧科学研究所 | Cryopreservation solution of horse sperms and freezing method thereof |
| CN103704202A (en) * | 2013-12-11 | 2014-04-09 | 广西大学 | Pony semen cryopreservation method |
| CN103651333A (en) * | 2013-12-23 | 2014-03-26 | 伊犁德瑞骏发生物科技有限公司 | Frozen semen diluent of equus animals and preparation method thereof |
| CN103843758A (en) * | 2014-02-19 | 2014-06-11 | 新疆维吾尔自治区畜牧科学院畜牧研究所 | Low temperature preservation diluent for horse sperm |
| CN103843758B (en) * | 2014-02-19 | 2015-09-23 | 新疆维吾尔自治区畜牧科学院畜牧研究所 | A kind of horse sperm Cord blood dilution |
| CN110583630A (en) * | 2019-10-22 | 2019-12-20 | 山东畜牧兽医职业学院 | Donkey semen diluent and preparation method thereof |
| CN112155785A (en) * | 2020-10-28 | 2021-01-01 | 内蒙古草原御驴科技牧业有限公司 | Healthy breeding method for improving mating success rate of donkey mothers |
| CN113273566A (en) * | 2021-05-31 | 2021-08-20 | 西北农林科技大学 | Application of glycine as effective component in boar semen normal-temperature preservation solution |
| CN114304139A (en) * | 2022-01-19 | 2022-04-12 | 山东畜牧兽医职业学院 | Semen preserving fluid for improving semen preserving quality and preparation method thereof |
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| CN101647427B (en) | 2012-11-28 |
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