CN101643215A - Preparation method of attapulgite used for mammalian cell culture - Google Patents
Preparation method of attapulgite used for mammalian cell culture Download PDFInfo
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- CN101643215A CN101643215A CN200910092180A CN200910092180A CN101643215A CN 101643215 A CN101643215 A CN 101643215A CN 200910092180 A CN200910092180 A CN 200910092180A CN 200910092180 A CN200910092180 A CN 200910092180A CN 101643215 A CN101643215 A CN 101643215A
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Abstract
The invention discloses a preparation method of attapulgite used for mammalian cell culture, belonging to the fields of attapulgite development and utilization technology and biotechnologies. The preparation method of the attapulgite used for a mammalian cell culture medium comprises the following steps: taking the attapulgite raw ore as raw materials, adopting a ball grinder to crash the attapulgite raw ore, classifying by a table concentrator, screening by a 200-mesh sieve to obtain primary powders of the attapulgite, then obtaining different grades of powders of the primary powders of the attapulgite by adopting a hydrochloric acidizing method, a polyacrylic acid dispersing method and an ultrasonic hydrothermal method, and repeating the steps to obtain the finished product. The preparation method broadens the application of the attapulgite in the bioindustry. The attapulgite used for mammalian cell culture has particle diameter being less than or equal to 500 meshes, high purity, good dispersion and sterility.
Description
Technical field
The invention belongs to attapulgite evaluation and exploration technology and biological technical field, the preparation method of attapulgite that provides a kind of mammalian cell substratum to use specifically.
Background technology
Attapulgite is a crystalloid hydrous magnesium aluminium silicate mineral, has the unique layer chain-like structure and the natural nano-material in duct.Attapulgite is because good surface, surface physics character and particular structure features such as its porousness, specific surface area are big, high adsorption capacity show as good colloid, absorption and catalytic performance.The attapulgite resource is rare relatively in the world, and China is the attapulgite resource than one of a few countries of horn of plenty, mainly concentrates on the area, Mingguang City in Xuyi, Jiangsu Province, Luhe County and Anhui.In the last few years, research to attapulgite both at home and abroad relates generally to its application and development, existing tens of the applicability patents about attapulgite, its industrialization reaches considerable scale, has obtained widespread use in industries such as building materials, mining, chemical fertilizer, food, agricultural chemicals, environmental protection and rubber.Because it all reaches nanometer scale at two-dimensional directional attapulgite, therefore obtain high purity, high dispersion nano level attapulgite, the nano effect, colloid effect and the adsorption effect that improve attapulgite are the keys of widening the Application Areas of attapulgite and realizing the economic worth that it is higher.
Owing to be subjected to the restriction of attapulgite size, impurity, up to now, do not see the report that the application of attapulgite in mammalian cell is cultivated arranged both at home and abroad, so utilize Chinese attapulgite resource, preparation is fit to the attapulgite particle of the particle diameter of mammalian cell cultivation, to widening the Application Areas of attapulgite, realization high technology content attapulgite is used in biological industry.
Summary of the invention
The purpose of this invention is to provide a kind of attapulgite particulate preparation method that mammalian cell is cultivated usefulness that can obtain in batches to be fit to, adopt ball mill with the attapulgite crushing raw ore, by the shaking table classification, obtain the elementary powder of attapulgite through 200 mesh sieves, then the elementary powder of the attapulgite of above-mentioned acquisition is adopted that hcl acidifying, polyacrylic acid are discrete, using supersonic, water-heating method and autoclaving combine, prepare and be used for the attapulgite method that the mammalian cell substratum is used, widen the application of attapulgite in biological industry.
Theoretical investigation shows that the adsorption layer that forms at the discrete particles surface dispersant can significantly change the Coulomb repulsion energy of dispersion system, sterically hindered repulsive energy and model ylid bloom action energy, increase considerably the repulsion potential energy between discrete particles, thereby improve the dispersion stabilization of suspended particle system.Therefore, utilize the different differences that cause of surface properties between dispersion system and impurity, enlarge their stably dispersing gender gap, utilize their differential settlements opposite sex just can reach isolating effect again dispersant adsorption; And the instantaneous pressure that the using supersonic, water-heating method utilizes ultrasonic wave to continue to form impacts the attapulgite aggregate surface, disperses its coacervate to reach the nano stick crystal level, effectively peels off and adheres to impurity between the attapulgite crystal, improves its dispersion efficiency.
For achieving the above object, the technical solution used in the present invention:
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 with the attapulgite of fragmentation, through 200 mesh sieves, obtains the elementary powder of attapulgite;
Step 2, selecting concentration for use is 1～5mol/l hydrochloric acid, the elementary powder of 1 attapulgite that obtains and the mass volume ratio of hydrochloric acid are 1: 10～1: 50 with g/ml set by step, in 40 ℃～60 ℃ constant temperature stir process 30～60 minutes, use the impurity in the elementary powder of deionized water flush away attapulgite and the chlorion of interpolation then, by centrifuge dehydration, oven dry, the secondary powder of acquisition attapulgite;
Step 3, selecting concentration for use is 1～10g/l polyacrylic acid solution, the secondary powder of 2 attapulgites that obtain and the mass volume ratio of polyacrylic acid solution are 1: 5～1: 50 with g/ml set by step, stir in 40 ℃～60 ℃ constant temperature and to be flocculent turbidity liquid to mixture in 30～60 minutes, and this flocculent turbidity liquid placed ultrasonic oscillator, the ultrasonic abundant aquation of secondary powder to attapulgite, polyacrylic acid with the adding of deionized water flush away, by centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4 is got three grades of powders of the attapulgite that step 3 obtains, and 2 carry out set by step, obtain the level Four powder of attapulgite;
Step 5 is got the level Four powder of the attapulgite that step 4 obtains, and 3 carry out set by step, obtain to be used for the attapulgite that mammalian cell is cultivated usefulness.
The rotating speed of described centrifuge dehydration is 5000～15000r/m.
The particle diameter of the attapulgite that is used for mammalian cell cultivation usefulness that the present invention obtains is smaller or equal to 500 orders.
When mammalian cell was cultivated, with the attapulgite of step 5 preparation, 115 ℃～135 ℃ of temperature, pressure was that 0.07～0.22MPa sterilized 30～60 minutes down.
The chlorion that adds in the described deionized water flush away attapulgite is that silver ions detects no chlorion.
Beneficial effect of the present invention: the present invention prepares the attapulgite that satisfies mammalian cell cultivation usefulness by pulverizing, acidifying with discrete combining, and realizes high added value attapulgite complete processing, has widened the Application Areas of attapulgite in biological industry.The present invention preparation be used for the attapulgite that mammalian cell is cultivated usefulness, the purity height, disperse, aseptic.
Description of drawings
The chemical structural drawing of Fig. 1 attapulgite.
The transmission electron microscope picture of Fig. 2 attapulgite.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment one
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 100 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.The elementary powder adding of attapulgite concentration is that the purpose of 1mol/l hydrochloric acid is to make elementary powder impurity of attapulgite and the effect of 1mol/l hydrochloric acid.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 80 milliliters of 1g/l polyacrylic acid, stirred 60 minutes in 60 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 50 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the level Four powder of attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 40 milliliters of 1g/l polyacrylic acid, stirred 60 minutes in 60 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, obtain the attapulgite that mammalian cell is cultivated usefulness.
Embodiment two
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 50 milliliters of 10mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 160 milliliters of 10g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 25 milliliters of 10mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the level Four powder of attapulgite;
Step 5, the attapulgite secondary powder 4g adding concentration of getting above-mentioned preparation is 80 milliliters of 10g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness;
Embodiment three
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 100 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 80 milliliters of 1g/l polyacrylic acid, stirred 60 minutes in 30 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 50 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 40 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the level Four powder of attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 40 milliliters of 1g/l polyacrylic acid, stirred 40 minutes in 30 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness.
Embodiment four
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 100 milliliters of 5mol/l hydrochloric acid, in temperature is 40 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 80 milliliters of 10g/l polyacrylic acid, stirred 60 minutes in 40 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 50 milliliters of 5mol/l hydrochloric acid, in temperature is 40 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 5000r/m centrifuge dehydration, oven dry, the level Four powder of acquisition attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 40 milliliters of 10g/l polyacrylic acid, stirred 60 minutes in 40 ℃ of constant temperature, place ultrasonic 30 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 5000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness.
Embodiment five
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 500 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 45 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 10000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.The elementary powder adding of attapulgite concentration is that the purpose of 1mol/l hydrochloric acid is to make elementary powder impurity of attapulgite and the effect of 1mol/l hydrochloric acid.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 40 milliliters of 1g/l polyacrylic acid, stirred 45 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 10000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 250 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 45 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 10000r/m centrifuge dehydration, oven dry, the level Four powder of acquisition attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 20 milliliters of 1g/l polyacrylic acid, stirred 45 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 10000r/m centrifuge dehydration, oven dry,, obtain the attapulgite that mammalian cell is cultivated usefulness.
Embodiment six
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 500 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 10000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 40 milliliters of 10g/l polyacrylic acid, stirred 60 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 10000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 250 milliliters of 1mol/l hydrochloric acid, in temperature is 60 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 10000r/m centrifuge dehydration,, the level Four powder of acquisition attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 20 milliliters of 10g/l polyacrylic acid, stirred 60 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 10000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness.
Embodiment seven
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, obtains the elementary powder of attapulgite through 200 mesh sieves.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 500 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 15000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 40 milliliters of 1g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 15000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 250 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 60 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 15000r/m centrifuge dehydration, oven dry, the level Four powder of acquisition attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 20 milliliters of 1g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 15000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness.
Embodiment eight
A kind of preparation method who is used for the attapulgite of mammalian cell cultivation usefulness, the preparation process of this method:
Step 1 is got the attapulgite ore, uses the stirring ball-milling crusher machine, through 200 mesh sieves, obtains the elementary powder of attapulgite.
Step 2, getting the elementary powder adding of 10g attapulgite concentration is 500 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 30 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 15000r/m centrifuge dehydration, oven dry obtains the secondary powder of attapulgite.
Step 3, with the attapulgite secondary powder 8g adding concentration of preparation is 40 milliliters of 10g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 15000r/m centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4, getting three grades of powders addings of 5g attapulgite concentration is 250 milliliters of 5mol/l hydrochloric acid, in temperature is 30 ℃ of stir process 30 minutes, then with the foreign ion in the abundant flush away attapulgite of deionized water and the chlorion of interpolation, by the 15000r/m centrifuge dehydration, oven dry, the level Four powder of acquisition attapulgite;
Step 5, the attapulgite level Four powder 4g adding concentration of getting above-mentioned preparation is 20 milliliters of 10g/l polyacrylic acid, stirred 30 minutes in 60 ℃ of constant temperature, place ultrasonic 60 minutes of ultrasonic oscillator to the abundant aquation of attapulgite particle then, with the polyacrylic acid in the abundant flush away attapulgite of deionized water, by the 15000r/m centrifuge dehydration, oven dry obtains the attapulgite that mammalian cell is cultivated usefulness.
Claims (3)
1. one kind is used for the preparation method that mammalian cell is cultivated the attapulgite of usefulness, it is characterized in that: the preparation process of this method:
Step 1 with the attapulgite of fragmentation, through 200 mesh sieves, obtains the elementary powder of attapulgite;
Step 2, selecting concentration for use is 1～5mol/l hydrochloric acid, the elementary powder of 1 attapulgite that obtains and the mass volume ratio of hydrochloric acid are 1: 10～1: 50 with g/ml set by step, in 40 ℃～60 ℃ constant temperature stir process 30～60 minutes, use the impurity in the elementary powder of deionized water flush away attapulgite and the chlorion of interpolation then, by centrifuge dehydration, oven dry, the secondary powder of acquisition attapulgite;
Step 3, selecting concentration for use is 1～10g/l polyacrylic acid solution, the secondary powder of 2 attapulgites that obtain and the mass volume ratio of polyacrylic acid solution are 1: 5～1: 50 with g/ml set by step, stir in 40 ℃～60 ℃ constant temperature and to be flocculent turbidity liquid to mixture in 30～60 minutes, and this flocculent turbidity liquid placed ultrasonic oscillator, the ultrasonic abundant aquation of secondary powder to attapulgite, polyacrylic acid with the adding of deionized water flush away, by centrifuge dehydration, oven dry is through three grades of powders of 500 mesh sieves acquisition attapulgite;
Step 4 is got three grades of powders of the attapulgite that step 3 obtains, and 2 carry out set by step, obtain the level Four powder of attapulgite;
Step 5 is got the level Four powder of the attapulgite that step 4 obtains, and 3 carry out set by step, obtain to be used for the attapulgite that mammalian cell is cultivated usefulness.
2. a kind of preparation method that mammalian cell is cultivated the attapulgite of usefulness that is used for according to claim 1, it is characterized in that: the rotating speed of described centrifuge dehydration is 5000～15000r/m.
3. a kind of preparation method that mammalian cell is cultivated the attapulgite of usefulness that is used for according to claim 1 is characterized in that: the mammalian cell that is used for that step 5 obtains is cultivated the attapulgite particle diameter of usefulness smaller or equal to 500 orders.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191696A (en) * | 2013-04-10 | 2013-07-10 | 合肥工业大学 | Method for improving adsorption property of attapulgite by using hydrothermal process |
| CN106337079A (en) * | 2016-08-31 | 2017-01-18 | 内蒙古田牧乳业有限公司 | Ice cream security detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103191696A (en) * | 2013-04-10 | 2013-07-10 | 合肥工业大学 | Method for improving adsorption property of attapulgite by using hydrothermal process |
| CN106337079A (en) * | 2016-08-31 | 2017-01-18 | 内蒙古田牧乳业有限公司 | Ice cream security detection method |
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