CN101629196A - Development and application of moso bamboo microsatellite molecular marker - Google Patents
Development and application of moso bamboo microsatellite molecular marker Download PDFInfo
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Abstract
The invention relates to the development of a moso bamboo microsatellite molecular marker, which comprises the following steps: obtaining 19 pairs of microsatellite molecular marker primers by three steps of obtaining a moso bamboo DNA sequence, screening a microsatellite locus and verifying primer design and a microsatellite locus; exploring the proper annealing temperature of the microsatellite molecular marker primers between 46 DEG C and 59 DEG C; applying the moso bamboo microsatellite primers in other six phyllostachys bamboos by DNA polyase chain reaction; amplifying, testing a sequence and confirming to obtain 102 phyllostachys DNA sequences containing the microsatellite locus. The method for developing the bamboo microsatellite molecular marker has the advantages of simplicity, high speed, stability, high efficiency and low cost and provides a new tool for the auxiliary breeding of bamboos.
Description
[technical field]
The present invention relates to a kind of plant dna molecule labeling technique, be specially the development technique of moso bamboo microsatellite molecular marker and in the application of other bamboo kinds of Phyllostachys.
[background technology]
Mao bamboon (Phyllostachys pubescens) is Gramineae (Gramineae) Bambusoideae (Bambusoideae) Phyllostachys (Phyllostachys) plant, it is one of topmost bamboo kind in China's bamboo resource, it distributes extensively, growth is fast, it is many to utilize, and has the important economy and the ecological value.How bamboo extend to main vegetative propagation with subterraneous stem owing to lack effective sexual breeding system, thereby conventional breeding technique can't be used, and causes the breeding process stagnation of bamboo, becomes the main bottleneck that bamboo industry develops.Marker assisted selection maybe will become the important means of bamboo breeding.Microsatellite marker (SSR wherein; Simple Sequence Repeat) conduct is with archaeal dna polymerase chain reaction (PCR; Polymerase Chain Reaction) technology and sequencing technologies are the molecule marker on basis; be the effective tool that makes up the High Density Molecular genetic map, analyzes species classification and evolution, chromosome structure, genetic diversity, kind protection and purity evaluation, be considered to the molecule marker that current inbred genetic Study on Variation intermediate-resolution is the highest, announcement power is the strongest.So far, the development and use of the microsatellite molecular marker in the bamboo plant are very few, and domestic have only Li Shuxian at 2002 annual interests paddy rice microsatellite marker primer random amplification bamboo genome, do not confirm as the report of the little satellite of bamboo but band is checked order; In addition, India report Nayak and Rout obtain 6 microsatellite molecular markers by making up and screen the genomic library of India thorn bamboo (Bambusa arundinacea).Still there is not the relevant report of moso bamboo microsatellite molecular marker both at home and abroad.
[summary of the invention]
Present situation at the still untapped moso bamboo microsatellite mark of prior art, the problem to be solved in the present invention is to utilize existing mao bamboon genome sequence in the public database, applying biological information science method, develop moso bamboo microsatellite molecular marker, and be applied to other bamboo kinds of Phyllostachys, further develop the microsatellite molecular marker that can be widely used in Phyllostachys bamboo kind.
The technical scheme that solves the present technique problem is:
The development approach of this moso bamboo microsatellite molecular marker comprises the steps: the acquisition of (1) mao bamboon dna sequence dna: the Latin formal name used at school of using mao bamboon is as keyword, and retrieval and download obtain in the geneseq database that NIH safeguards; (2) contain the screening of microsatellite DNA sequence: with little satellite routine analyzer of standalone version and online microsatellite locus analysis software, the sequence of downloading is screened, obtain to contain the mao bamboon dna sequence dna of microsatellite locus; (3) design of primers and microsatellite locus checking: 1. design of primers: the archaeal dna polymerase chain reaction primer that contains microsatellite locus with primer-design software design amplification, primer contains the base numerical control built in 18-22, two primer annealing temperature head＜4 ℃, the dna fragmentation of amplification is in 350 bases; 2. little satellite checking: utilize above-mentioned primer to carry out amplification again, subclone and order-checking affirmation work that detection, microsatellite locus are dyed in archaeal dna polymerase chain reaction amplification, the electrophoretic separation of amplified production, silver, obtain 19 pairs of moso bamboo microsatellite labeled primers at last, its annealing temperature is at 46 ℃-59 ℃.
The primer that obtains 19 pairs of moso bamboo microsatellite marks is respectively:
PBM002：5’-ATGGAGCATTCATTTGAG-3’ 5’-TTGACAAGTTTGGAGGGT-3’
PBM004：5’-CGATGAAGTTGCCTGTGG-3’ 5’-TCAAGTCGCATTGGAGTA-3’
PBM007：5’-CTCGAGGTGAAGTTACATTA-3’?5’-TCGTAGAGCACTATCCATTT-3’
PBM011：5’-TAAAGAAGTATAATGTATTCG-3’5’-AGCTGTTTTAGCATCTATGTT-3’
PBM013：5’-TTTTACTAACGTGCCCGTATT-3’5’-GGAGGATCGCTGGATTGG-3’
PBM014：5’-GCCGTCCAAACGCTCCTT-3’ 5’-CACCCATCCATCTTATGCTAT-3’
PBM016：5’-CCCACAGAAGGTCGCACTA-3’ 5’-CCGCCGAGAACGTCTACAT-3’
PBM017：5’-TATGCCTCCAATAATCCG-3’ 5’-AAGGCGTAGCCACCGAAT-3’
PBM018：5’-GACGACGAGTGTAACGCTGAG-3’5’-AGGATGATGCCGGTGAGG-3’
PBM019：5’-TCCTAGCCCTACCCTGTCC-3’ 5’-CACGGTGCTCGCTTAAATAG-3’
PBM020：5’-TCGCATCTAAACAACATC-3’ 5’-GAGAAGAAGAAAGGAGGA-3’
PBM021：5’-AGGGTGTTATTTGCTATTGT-3’?5’-AAATCCGACGCTGGAGGC-3’
PBM022：5’-AAAGATGTTGCCTTGTTC-3’ 5’-GTGCATGTCTATTCCCTC-3’
PBM023：5’-TTTCATGGAGGGCGACAG-3’ 5’-GCCGACCAGGAAACAAACGAA-3’
PBM025：5’-GTTGTGCCCAGCACTTTA-3’ 5’-TGACGCCACTCATTTCTT-3’
PBM026：5’-CTATCATCAAATGTGCCTCAA-3’5’-CCGAGCCTCACTTTCTGC-3’
PBM027：5’-AAAGCAACCACGCCATTA-3’ 5’-TCGGAGGAGAAGGAGAAG-3’
PBM028：5’-CAGGTAGTCGTCTTATCGAGT-3’5’-ATAAGTCAATTTCGAATGAA-3’
PBM030：5’-GTTGCCCGTGGTTACTGT-3’ 5’-CGTGAGCCATCTGAGTTT-3’
Numbering is respectively PBM002, PBM004, PBM007, PBM011, PBM013, PBM014, PBM016, PBM017, PBM018, PBM019, PBM020, PBM021, PBM022, PBM023, PBM025, PBM026, PBM027, PBM028, the annealing temperature of the primer at little satellite nucleotide sequence two ends of PBM030 is respectively 48 ℃, 48 ℃, 53 ℃, 50 ℃, 53 ℃, 52 ℃, 56 ℃, 55 ℃, 52 ℃, 59 ℃, 46 ℃, 55 ℃, 47 ℃, 58 ℃, 47 ℃, 50 ℃, 49 ℃, 53 ℃, 50 ℃.
Said primer is applied to the exploitation that an ancient plucked stringed instrument bamboo, black bud bamboo, Lei Zhu, fishscale bamboo, false mao bamboon and 6 Phyllostachys of phyllostachys bambusoides are represented the little satellite of bamboo kind, confirms that by amplification and order-checking having obtained 102 Phyllostachys dna sequence dnas that contain microsatellite locus sees Table (2).
The invention has the beneficial effects as follows development approach with respect to other little satellites, have advantages such as simple, quick, stable, efficient and low cost, for the marker assisted selection of Phyllostachys bamboo kinds such as mao bamboon provides strong instrument, help bamboo plant genetics and molecular biological further research simultaneously.
[embodiment]
Below the invention will be further described, the exploitation of moso bamboo microsatellite molecular marker of the present invention and using through the following step:
One, the acquisition of mao bamboon sequence
Latin formal name used at school " Phyllostachys pubescens " with mao bamboon is a keyword, the geneseq database of safeguarding in NIH (GenBank:http: //www.ncbi.nlm.nih.gov/) retrieve, logining of obtaining the mao bamboon dna sequence dna all download with the .fasta document form.
Two, the screening of mao bamboon dna sequence dna
Utilize standalone version microsatellite locus routine analyzer (MISA) and online microsatellite locus analysis software (Microsatellite Repeats Finder), the dna sequence dna of downloading is screened, obtain containing the mao bamboon dna sequence dna of microsatellite locus.
Three, design of primers and the checking of moso bamboo microsatellite site
1, design of primers
Containing on the dna sequence dna of microsatellite locus, utilize primer-design software Primer Premier 5 (PRIMIER Biosoft International, CA, USA) design dna polymerase chain reaction (PCR) amplification primer, the size of design primer is about 20 bases, expanding fragment length is in the 100-300 base, and the annealing temperature of two primers differs and is no more than 4 ℃.In a dna sequence dna, contain two microsatellite locus, and during apart from each other, primer is designed in each site respectively; When two microsatellite locus are nearer in same sequence, just make two sites in the amplification scope of a pair of primer during design of primers apart as far as possible.
2, the microsatellite locus checking
1. archaeal dna polymerase chain reaction amplification
By the PCR method microsatellite marker primer amplification mao bamboon genomic dna that designs, amplification reaction system and program are as follows: reaction system is 20 microlitres, comprising: 10 * damping fluid, 2 microlitres, MgCl
2(25 mmoles/liter) 1.2 microlitres, deoxyribonucleotide (10 mmoles/liter) 0.6 microlitre, archaeal dna polymerase (5 units/microlitre) 0.25 microlitre, dna profiling 50-100 nanogram, primer (25 mmoles/liter) 0.5 microlitre.The response procedures of polymerase chain reaction is: 94 ℃ of pre-sex change 5 minutes, 32 circulations (94 ℃ of sex change 40 seconds, renaturation is 40 seconds under each primer annealing temperature, 72 ℃ were extended 90 seconds), last 72 ℃ are extended 4 ℃ of preservations after 10 minutes.Thereby acquisition amplified production.The annealing temperature of each primer sees table 1 for details.
2. electrophoretic separation and silver dye detection
Add the sex change damping fluid in the amplified production, with 4% denaturing polyacrylamide gel (0.4 millimeter of thickness) and 1 * TBE electrophoretic buffer, in the sequencing gel electrophoresis system that U.S. C.B.S. company produces, carry out, prerunning go up after 30 minutes 100 watts of permanent power electrophoresis of sample after about 2 hours silver dye detection, the silver staining method process is mainly as follows: after electrophoresis finishes, the sheet glass that is stained with gel inserted be used for the vinyl disc that silver dyes; Add 10% acetic acid, slight concussion was fixed in 30 minutes on shaking table, added rinsed with deionized water then 3 times, each 5 minutes; Gel is inserted in the dyeing dish of 0.1% cma staining liquid, slight concussion is 30 minutes on shaking table, uses 10 seconds of rinsed with deionized water then; Gel is inserted in the colour developing dish of 0.32% yellow soda ash, slightly shaking on the shaking table until band till clear and band number no longer increases; Add stationary liquid, the color development stopping reaction is with distilled water rinsing several minutes; Remove the globule on gel and the sheet glass, be placed on and observe on the opalescence lamp box and take pictures with digital camera.
3. the amplification again and the subclone of microsatellite locus
According to the peculiar electrophoretic band feature preliminary judgement electrophoresis picture of microsatellite locus, and by further affirmation of rubber tapping order-checking.Concrete step is: cut off the band that the silver in the polyacrylamide gel dyes on the strongest microsatellite locus of signal and place pipe, add 10 microlitre TE solution (10 mmoles/rise Tris-HCl, 1 mmole/rise EDTA, pH8.0) back on the PCR instrument 94 ℃ the heating 30 minutes, the centrifuging and taking supernatant liquor is as the secondary PCR template then, under PCR reaction system and response procedures same as described above, increase with the corresponding micro-satellite primers in each site, amplified production separates with 1% sepharose, the purpose band is reclaimed test kit (QIAquick Gel Extraction Kit with glue, QIAGEN) reclaim, reclaim product and carry out 16 ℃ of connections of spending the night with pMD18-T carrier (TAKARA), carry out subclone after transforming Top10 competent cell (TAKARA), last picking positive colony is cultivated, (QIAGEN Plasmid Mini Kit QIAGEN) checks order behind the extraction plasmid with test kit with the plasmid extraction.
4. order-checking is confirmed
With the plasmid of above-mentioned gained, (BigDye Terminator v3.1Cycle SequencingKit ABI) does order-checking PCR reaction on 96 hole PCR plates with sequencing kit.Reaction system is 20 microlitres, comprising: plasmid DNA, 1 microlitre (200 nanogram); BigDye Terminator v3.1Ready Reaction mix, 1 microlitre; 5 * order-checking damping fluid, 4 microlitres; Sequencing primer (20 micromoles per liter), 1 microlitre; The sterilization deionized water, 13 microlitres.Reaction conditions is: 96 ℃ 1 minute, 25 circulations (96 ℃ 10 seconds, 50 ℃ 5 seconds, 60 ℃ 4 minutes), last 4 ℃ of insulations.
The PCR reaction finishes the back and with alcohol/EDTA/NaAc method the order-checking product is carried out purifying.Concrete step is as follows: add the 125 mmoles/microlitre EDTA of 2 microlitres, the 3 mmoles/microlitre NaAc and 50 microlitres, 100% alcohol of 2 microlitres in each reaction tubes, and concussion mixing 4 times, room temperature was placed 15 minutes; 3,000 centrifugal force are inverted 96 orifice plates after centrifugal 30 minutes, centrifugal stop during to 185 centrifugal force centrifugal; Every pipe adds 70% alcohol of 70 microlitres, and 1,500 centrifugal force is inverted 96 orifice plates down for 4 ℃ after centrifugal 15 minutes, centrifugal stop during to 185 centrifugal force centrifugal; Repeat the centrifugal process 1 time under 1,500 centrifugal force; The room temperature clean alcohol that volatilizees adds 10 microlitre deionized formamide dissolving DNAs; Sample after the dissolving is put rapidly and is cooled off in the ice after 4 minutes 95 ℃ of sex change 4 minutes, and last sample is gone up order-checking at the sequenator (ABI 3100 AvantGenetic Analyzer) that ABI company produces.
Four, moso bamboo microsatellite molecular marker is in the application of other bamboo kinds of Phyllostachys
Dye steps such as the amplification again and the subclone of detection, microsatellite locus, the affirmation of checking order, concrete grammar such as above-mentioned 32 by PCR method with other 6 bamboo kind genomic dnas of moso bamboo microsatellite labeled primer amplification Phyllostachys, electrophoretic separation and silver.
Table 1 moso bamboo microsatellite molecular marker archaeal dna polymerase chain reaction amplimer and annealing temperature thereof
Table 2: the exploitation of moso bamboo microsatellite molecular marker and applying gene sequence table
＜110〉Zhejiang Forestry Institute
＜120〉exploitation of moso bamboo microsatellite molecular marker and application
＜130〉plant dna molecule genetic marker technology
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<223>
<400>7
cgatgaagtt?gcctgtgcct?gctgtatcat?cctccttatg?ctgcgtgaac?gaccccccga 60
aagtggctga?gggactacta?actaactgac?taactatata?tatatatata?tatatatata 120
tatatatata?tatatatata?tatatatata?tatatatata?ggacgtccgt?ttcgtactcc 180
tgggagtatg?tactcccact?ttcaatatac?cccttaaatg?aatatattat?acttctttat 240
cactttcagt?atatttaaat?accaattata?agatactcca?atgcgacttg?a 291
<210>8
<211>278
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(69)..(94)
<223>
<220>
<221>repeat_region
<222>(97)..(144)
<223>
<400>8
cgatgaagtt?gcctgtggct?gctgtctcat?tctcctcacg?acccctcgaa?agtggctcga 60
ggactacgac?acacacacac?acacacacac?acacgcacac?acacacacac?acacacacac 120
acacacacac?acacacacac?acacatacga?tgcccgtttt?gtactcctgg?gagtacatac 180
ttccacttcg?aatacaccac?ttaaacgaat?atattatatt?tcattatcat?ttttagtata 240
cttaaatacc?aattataaga?tactccaatg?cgacttga 278
<210>9
<211>231
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(84)..(101)
<223>
<400>9
cgatgaagtt?gcctgtggct?actgtctcat?ccttctcatg?ctgcgtggat?gacccctcga 60
aagtggctga?gggactacga?acaatatata?tatatatata?tgacgctcgt?ttcgtactcc 120
cgggagtacg?tactcccact?tcgaatatac?cacttaaacg?aatatattat?acttctttat 180
cactttcagt?atacttaaat?accaattata?agatactcca?atgcgacttg?a 231
<210>10
<211>211
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(70)..(79)
<223>
<400>10
cgatgaagtt?gcctgtggct?actatctcat?cctcacgaca?ccccgaaagt?ggccggggga 60
ctacgaacaa?tatatatata?agacacccgt?ttcgtactcc?tgggagtaca?tactcccact 120
ttcaatatac?cacttaaacg?gatatattat?acttctttat?cattttcagt?atacttaaat 180
accaattata?agatactcca?atgcgacttg?a 211
<210>11
<211>277
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(84)..(119)
<223>
<220>
<221>repeat_region
<222>(120)..(145)
<223>
<400>11
cgatgaagtt?gcctgtggct?gttgtctcat?ccttctcatg?ctgtgtggac?gaccccccga 60
aagtggctga?gggactacga?acaatatata?tatatatata?tatatatata?tatatatata 120
gagagagaga?gagagagaga?gagagaagat?gcccgtttcg?tactcctagg?agtatgtact 180
cccactttga?atataccact?taaatgcata?tattatactt?ctttatcact?ttcagtacag 240
ttaaatacca?agtataagat?actccaatgc?gacttga 277
<210>12
<211>219
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(74)..(83)
<223>
<400>12
cgatgaagtt?gcctgtgttt?attgtttccc?cctcctcatg?ctgagtggac?gaccatccga 60
aagtggctga?gggagagaga?gagacggggg?atgcccgttt?cgtactccta?ggagtatgta 120
ctcccacttt?gaatatacca?cttaaatgca?catattatac?ttctttatca?ctttcagtac 180
agttaaatac?caattataag?atactccaat?gcgacttga 219
<210>13
<211>216
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(75)..(84)
<223>
<400>13
cgatgaagtt?gcctgtggct?gctgtctccc?cctcctcacg?accccccgaa?agtggctggg 60
ggactacgaa?ctctatatat?atataggact?cccgtttcgt?actcctgaga?gtacatactc 120
ccactttgga?tataccactt?aaacaaatat?attatacttc?tttatcattt?tcagtatact 180
taaataccaa?ttataagata?ctccaatgcg?acttga 216
<210>14
<211>179
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(91)..(114)
<223>
<400>14
ctcgaggtga?agttacatta?attaatatta?attatatcag?agatgattta?gcggaacaaa 60
ttaattaatt?atatatatat?ccgatctgtg?atatatatat?atatatatat?atatagttaa 120
agcaaatttt?cagcattatt?taaatgacaa?aattgatgaa?aatggatagt?gctctacga 179
<210>15
<211>178
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(80)..(113)
<223>
<400>15
taaagaagta?taatgtattc?gttccaacgg?tatattggaa?gtgggagtac?atactcccaa 60
gagtacgaaa?cgagcgtcct?atatatatat?atatatatat?atatatatat?atatatatat 120
atatatatat?atatgtcaag?cctaaaaatg?tttgactaac?atagatgcta?aaacagct 178
<210>16
<211>176
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(80)..(119)
<223>
<220>
<221>repeat_region
<222>(120)..(131)
<223>
<400>16
taaagaagta?taatgtattc?gttcaaacgg?tatattggaa?gtgggagtac?atactcccaa 60
gagtacgaaa?cgagcgtcct?atatatatat?atatatatat?atatatatat?atatatatat 120
gtatatatat?atgtcaagcc?taaaaatgtt?tgactaacat?agatgctaaa?acagct 176
<210>17
<211>151
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(80)..(93)
<223>
<400>17
taaagaagta?taatgtattc?gttaaagtgg?tatattggaa?gtgggagtac?atactcccaa 60
gagtacgaaa?caggcgtcct?atatatatat?atattgattg?attgattgtc?aagcctaaaa 120
atgtttgact?aacatagatg?ctaaaacagc?t 151
<210>18
<211>229
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(86)..(117)
<223>
<220>
<221>repeat_region
<222>(120)..(159)
<223>
<400>18
taaagaagta?taatgtattc?gtttccaagt?ggtatattgg?aagtgggagt?acatactccc 60
aagagtacga?aacaggcgtc?ctacatatat?atatatatat?atatatatat?atatatatgt 120
atatatatat?atatatatat?atatatatat?atatatatag?acacgacatg?attgattgat 180
tgattgtcaa?gcctaaaaat?gtttgactaa?catagatgct?aaaacagct 229
<210>19
<211>161
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(120)..(129)
<223>
<400>19
ttttactaac?gtgcccgtat?ttaggatggt?tttgctgatg?tgtcacgcag?acagacggat 60
gtggcgctcc?tagacatctc?ctcacatcac?gttttcttct?tcacatcacc?ttttcttccc 120
atctctctct?attcctctat?ccccaatgcc?agcgatcctc?c 161
<210>20
<211>163
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(122)..(131)
<223>
<400>20
ttttactaac?gtgcccgtat?ttagggtggt?ttcgctgatg?tgtcacgctg?tgaggcggac 60
gtggcgctcc?tagtcgtctc?ctcacatcat?gttttcttcc?tcacatcacc?ttttcttccc 120
atctctctct?ctattcctgt?atccccaatg?ccagcgatcc?tcc 163
<210>21
<211>162
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(122)..(129)
<223>
<400>21
ttttactaac?gtgcccgtat?ttaggatggt?tttactgatg?tgtcacgctg?acagacggat 60
gtggcgctcc?tagtcatctc?ctcacatcac?gttttcttct?tcacatcacc?ttttcttccc 120
atctctctct?atttcctcta?tccccaatgc?cagcgatcct?cc 162
<210>22
<211>171
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(122)..(129)
<223>
<400>22
ttttactaac?gtgcccgtat?ttagggtggt?tttgctgatg?tgtcacgctg?tcaggcggac 60
gtggcgctcc?tagtcatctc?ctcacatcat?gttttcttcc?tcacatcacc?ttttcttccc 120
atctctctct?ctctctctct?attcctctat?ccccaatgcc?agcgatcctc?c 171
<210>23
<211>292
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(27)..(46)
<223>
<220>
<221>repeat_region
<222>(176)..(187)
<223>
<400>23
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?ctctctcagc?tcttcctcct 60
cagctcgccg?tcgccgtcgc?ctgatcgttc?accgcttcgc?ttcacaggga?tcagtgctgt 120
gacagttgct?gtgtggaact?cttgttttag?tgttgtatcc?acatttatga?tgtatcatca 180
tcatcatctt?cttttttgaa?gtatttgttt?ttctttcttg?tgcttgatat?ggtatggcaa 240
agttgaagag?gcaggcagtg?aacaaaagta?tatagcataa?gatggatggg?tg 292
<210>24
<211>244
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(27)..(34)
<223>
<400>24
gccgtccaaa?cgctccttct?tctgaactct?ctctcggctc?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcgcagg?gatcagtgat?gtgatagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tatccacatg?tatttgaagt?ctttgttttt?tctttcctgt 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>25
<211>283
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(27)..(40)
<223>
<220>
<221>repeat_region
<222>(170)..(178)
<223>
<400>25
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?cagctcttcc?tcagctcgcc 60
gtcgccgtcg?cctgatcgtt?caccgcttcg?cttcacaggg?atcagtgctg?tgacagttgc 120
tgtgtggaac?tcttgtttta?gtcttgtatc?cacatttatg?atgtattatc?atcatcatct 180
tcttttttta?agtatttgtt?tttctttctt?gtgcttgata?tggtatggca?aagttgaaga 240
ggcagacagt?gaacaaaagt?atatagcata?agatggatgg?gtg 283
<210>26
<211>244
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(27)..(34)
<223>
<400>26
gccgtccaaa?cgctccttct?tctgaactct?ctctcggccc?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcacagg?gatcagtgat?gtgatagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tatccacatg?tatttgaagt?ctttgttttt?tctttcctgt 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>27
<211>274
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(27)..(40)
<223>
<220>
<221>repeat_region
<222>(158)..(169)
<223>
<400>27
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?cccagctcgc?cgtcgccgtc 60
gcctgatcgt?tcaccgcttc?gcttcacagg?gatcagtgct?gtgacagttg?ctgtgcggaa 120
ctcttgtttt?agtgttgtac?ccacatttat?gatgtatcat?catcatcatc?ctctttttta 180
aagtatttgt?ttttctttct?tgtgcttgat?atggtatggc?aaagttgaag?aggcagacag 240
tgaacaaaag?tatatagcat?aagatggatg?ggtg 274
<210>28
<211>244
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(27)..(34)
<223>
<400>28
gccgtccaaa?cgctccttct?tctgaactct?ctctcggccc?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcacagg?gatcagtgat?gtgatagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tatccacatg?tatttgaagt?ctttgttttt?tctttcctgt 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>29
<211>274
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(27)..(42)
<223>
<220>
<221>repeat_region
<222>(161)..(169)
<223>
<400>29
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?ctcagctcgc?cgtcgccgtc 60
gcctgatcgt?tcaccgcttc?gcttcacagg?gatcagtact?gtgacagttg?ctgtgtggaa 120
ctcttgtttt?agtgttgtat?ccacatttat?gatgtattac?catcatcatc?ttcttttttg 180
aagtatttgt?ttttctctct?tgtgcttgat?atggtatggc?aaagttgaag?aggcagatag 240
tgaacaaaag?tatatagcat?aagatggatg?ggtg 274
<210>30
<211>244
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(27)..(34)
<223>
<400>30
gccgtccaaa?cgctccttct?tctgaactct?ctctcggctt?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcacagg?gatcagtgat?gtgatagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tatccacatg?tatttgaagt?ctttgttttt?tctttcctgc 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>31
<211>316
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(27)..(68)
<223>
<220>
<221>repeat_region
<222>(201)..(212)
<223>
<400>31
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?ctctctctct?ctctctctct 60
ctctctctca?gctcttcctc?ctcagctcgc?cgtcgccgtc?gcctgatcgc?tcaccgcttc 120
gcttcacagg?gatcagtgct?gtgacagttg?ctgtgtggaa?ctcttgtttt?agtgttttat 180
ccacatttat?gatgtattat?catcatcatc?atcttctttt?tgaagtattt?gtttttcttt 240
cttgtgcttg?atatggtatg?gcaaagttga?agaggcagac?agtgaacaaa?agtatatagc 300
ataagatgga?tgggtg 316
<210>32
<211>298
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(27)..(50)
<223>
<220>
<221>repeat_region
<222>(180)..(194)
<223>
<400>32
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?ctctctctct?cagctcttcc 60
tcctcagctc?gccgtcgccg?tcgcctgatc?gttcaccgct?tcgcttcaca?gggatcagtg 120
ctgtgacagt?tgctgtgtgg?aactcttgtt?ttagtgttgt?atccacattt?atgatgtatc 180
atcatcatca?tcatcttctt?tttgaagtat?ttgtttttct?ttcttatgct?tgatatggta 240
tggcaaagtt?gaagaggcag?acagtgaaca?aaagtatata?gcataagatg?gatgggtg 298
<210>33
<211>244
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(27)..(34)
<223>
<400>33
gccgtccaaa?cgctccttct?tctgaactct?ctctcggctc?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcacagg?gatcagtgat?gtgatagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tacccacatg?tatttgaagt?ctttgttttt?tctttcctgt 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>34
<211>294
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(69)..(78)
<223>
<400>34
gccgtccaaa?cgctccttct?tctgaactct?ctctctctct?ctctctctca?gctcttcctc 60
ctcagctcgc?cgtcgccgtc?gcctgatcgt?tcaccgcttc?gcttcacagg?gatcagtgct 120
gtgacggttg?ctgtgtggaa?ctcttgtttt?agtgttgtat?ccacatttat?gatgtattat 180
catcatcatc?ttcttttttg?aagtatttgt?ttttctttct?tgtgcttgat?atggtatggc 240
aaagttgaag?aggcagacag?tgaacaaaag?tatatagcat?aagatggatg?ggtg 294
<210>35
<211>244
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(27)..(48)
<223>
<220>
<221>repeat_region
<222>(181)..(189)
<223>
<400>35
gccgtccaaa?cgctccttct?tctgaactct?ctctcggctc?ttcctcctct?gctcgccgtc 60
gccggatcgt?tcaccgcttc?gcctcacagg?gatcagtgat?gtagtagtag?ttgctgtgtg 120
gaactcttgt?tttagtgttg?tatccacatg?tatttgaagt?ctttgttttt?tctttcctgt 180
gtttcatatg?gtattgcaaa?gttgaaggga?cagacagaag?tacatagcat?aagatggatg 240
ggtg 244
<210>36
<211>104
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(23)..(58)
<223>
<400>36
cccacagaag?gtcgcactat?acagagagag?agagagagag?agagagagag?agagagaggc 60
acaggatcgg?gatggcgata?ccttgatgta?gacgttctcg?gcgg 104
<210>37
<211>94
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(23)..(48)
<223>
<400>37
cccacagaag?gtcgcactat?acagagagag?agagagagag?agagagaggc?acaggatcgg 60
gatggcgata?ccttgatgta?gacgttctcg?gcgg 94
<210>38
<211>86
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(23)..(40)
<223>
<400>38
cccacagaag?gtcgcactat?acagagagag?agagagagag?gcacaggatc?gagatggcga 60
taccttgatg?tagacgttct?cggcgg 86
<210>39
<211>88
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(23)..(40)
<223>
<400>39
cccacagaag?gtcgcactat?acagagagag?agagagagag?gggcacagga?tcaggatggc 60
gataccttga?tgtagacgtt?ctcggcgg 88
<210>40
<211>88
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(23)..(40)
<223>
<400>40
cccacagaag?gtcgcactat?acagagagag?agagagagag?aggcacagga?tcgagatggc 60
gataccttga?tgtagacgtt?ctcggcgg 88
<210>41
<211>126
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(66)..(73)
<223>
<400>41
cccacagaag?gtcgcactac?agccacagcc?cttacaggaa?tcgtctcgca?atcaagggac 60
gcactagaga?gaggaaggca?cacagggcgc?gggacggcga?taccttgatg?tagacgttct 120
cggcgg 126
<210>42
<211>242
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(93)..(119)
<223>
<400>42
tatgcctcca?ataatccgct?caacgtcata?tctattaaaa?gtgacccggg?agcttggggt 60
cggaggtgag?gatgatcttg?aacgacacct?tccctcctcc?tcctcctcct?cctcctcctg 120
ccgcggcgcc?gccgcccgcc?gccatggggt?gtgggtgttg?ggggaggggg?gtcgagacgc 180
cgagtggttc?acctcacctc?ggttgcggtt?tgcgtgatcg?atagattcgg?tggctacgcc 240
tt 242
<210>43
<211>230
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(92)..(112)
<223>
<400>43
tatgcctcca?ataatccgct?caacgtcata?gctattaaaa?gtgaagggga?gcttggggtc 60
ggaggtgagg?atgatcttga?acgacacctt?ccctcctcct?cctcctcctc?ctaccgcgcc 120
gccgccacct?gccgccatgg?agtgtgggtg?ttgggggggt?cgagacgccg?agtggttcgc 180
ctcgcctcgg?tcacggtttg?cgtgatcgat?agattcggtg?gctacgcctt 230
<210>44
<211>240
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(92)..(118)
<223>
<400>44
tatgcctcca?ataatccgct?caacgtcaca?gctattaaaa?gtgaagggga?gcttggggtc 60
ggaggtgagg?atgatcttga?acgacacctt?ccctcctcct?cctcctcctc?ctcctcctcc 120
cgccgcggtg?tcgtcgccgc?catggggtgt?gggtgttgag?gggggggggt?cgagacgccg 180
agtggttcgc?ctcgcctcgg?tcgcggtttg?cgtgatcgat?agattcggtg?gctacgcctt 240
<210>45
<211>245
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(93)..(112)
<223>
<400>45
tatgcctcca?ataatccgct?caacgtcata?gctattaaaa?gtgaagcggg?agcttggggt 60
cggaggtaag?gatgatcttg?aacgacacct?tccctcctcc?tcctcctcct?cctcctcctc 120
ctgccgcggc?gccgccgccc?gccgccatgg?ggtgtgggtg?ttgggggagg?ggggtcgaga 180
cgccgagtgg?ttcacctcac?ctcggttgcg?gtttgcgtga?tcgatagatt?cggtggctac 240
gcctt 245
<210>46
<211>230
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(92)..(112)
<223>
<400>46
tatgcctcca?ataatccgct?caacgtcaca?tctattaaaa?gtgaagggga?gcttggggtc 60
ggaggtgagg?atgatcttga?acgacacctt?ccctcctcct?cctcctcctc?ctaccgcgcc 120
gccgccacct?gccgccatgg?agtgtgggtg?ttgggggggt?cgagacgccg?agtggttcgc 180
ctcgcctcgg?tcacggtttg?cgtgatcgat?agattcggtg?gctacgcctt 230
<210>47
<211>285
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>47
gacgacgagt?gtaacgctga?gaagtcctca?aaatcccctc?taggtcagtc?atgctctcca 60
tcagtgcaga?catcctatta?tgagtttcct?caaagaaatc?attctgaatt?tgctccatct 120
caagtaatct?gcctcaagtc?tcattctgaa?attcaacttg?ttcggccttg?atttcttgca 180
aagagctaag?aatgagctgc?tgattttgaa?gaatctgtgc?aagagtgcta?tcagcaagaa 240
catcagcagc?agtagcaaca?gcaggaacct?caccggcatc?atcct 285
<210>48
<211>285
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>48
gacgacgagt?gtaacgctga?ggggtcctca?aactctctgc?caagtcatga?aagctctcca 60
tcagggcagg?catcctgtta?tgagactcat?cgaagaaatc?attttgagtt?cgctccatct 120
cacgtaatct?gcctcgagtc?tcactctgaa?attcagcttg?ttcagccttg?atttcttgca 180
aagagctaag?tatgagttgc?tgattctgaa?gaatctatgc?aagagtgcta?tcagcaggag 240
catcaacagc?agtagcaata?gcaggagcct?caccggcatc?atcct 285
<210>49
<211>285
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>49
gacgacgagt?gtaacgctga?ggagtcctca?aaatcccctc?taggtcactc?atgctctcca 60
tcagtgcaga?catcctatta?tgagtttcct?caaagaaatc?attctgaatt?tgctccatct 120
caagtaatct?acctcgagtc?tcattctgaa?attcaacttg?ttcagccttg?atttcttgca 180
aagagctaag?aatgagctgc?tgattttgaa?gaatctatgc?aagagtgcta?tcagtaggaa 240
catcagcagc?agtagcaaca?gcaggaacct?caccggcatc?atcct 285
<210>50
<211>285
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>50
gacgacgagt?gtaacgctga?ggagtcctca?aaatcccctc?taagtcagtc?atgctctcca 60
tcagtgcaga?catcctatta?tgagtttcct?cagagaaatc?attctgaatt?tgctctatct 120
caagtaatct?tcctcgagtc?tcattctgaa?attcagcttg?ttcagtcttg?atttcttgca 180
aagagctaag?aatgagctgc?tgattttgaa?gaatctatgc?aagagtacta?tcagcaggaa 240
catcagcagc?agtagcaaca?gcaggagcct?caccggcatc?atcct 285
<210>51
<211>285
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>51
gacgacgagt?gtaacgctga?ggagtcctca?aaatcccctc?taggtcagtc?atgctctcca 60
ttagtgcaga?catcctatta?tgagtttcct?caaagaaatc?attctgaatt?tgctccatct 120
caagtaatct?gcctcgagtc?tcattctgaa?attcaacttg?ttcagccttg?atttcttgca 180
aagagctaag?aatgagctgc?tgattttgaa?gaatctgtgc?aagagtgcta?tcagtaggaa 240
catcagcagc?agtagcaaca?gcaggaacct?caccggcatc?atcct 285
<210>52
<211>285
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(244)..(252)
<223>
<400>52
gacgacgagt?gtaacgctga?ggggtcctca?aactctctgc?caagtcatga?aagctctcca 60
tcagggcaga?catcctgtta?tgagactcat?cgaagaaatc?attctgagtt?cgctccatct 120
cacgtaatct?gccttgagtc?tcactctgaa?attcagcttg?ttcagccttg?atttcttgca 180
aagagctaag?aatgagttgc?tgattctgaa?gaatctgtgc?aagagtgcta?tcagcaggag 240
catcagcagc?agtagcaata?gcaggagcct?caccggcatc?atcct 285
<210>53
<211>189
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(141)..(155)
<223>
<400>53
tcctagccct?accctgtccc?cttttcggtc?acgccgcccg?tccctgcctc?cgccgcgccc 60
gagcgccccc?tttttctgcc?ccttctccgt?ggatctcgcc?gcttctcgtc?gcccgccatt 120
aaggccaccg?cggcaagcct?ctccctcctc?ctcctccggc?ccccctagcc?tatttaagcg 180
agcaccgtg 189
<210>54
<211>202
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(154)..(165)
<223>
<400>54
tcctagccct?accctgtccc?gtctccaccg?cggcgtcacc?tcccgcctcc?gccgcactcg 60
ctgccgcttc?gcccatgcct?tggcaccgcg?ccatacctcc?tccccatgcc?gcgcgggtca 120
agccgccatt?aatggccgac?tcgactgcca?tgcctcctcc?tcctcctggg?caccccagtc 180
gcctatttaa?gcgagcaccg?tg 202
<210>55
<211>191
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(138)..(152)
<223>
<400>55
tcctagccct?accctgtccc?gtctcccccg?tgccatccgt?ccccgcctcc?gtcgcgcccg 60
tgcgccctct?ttttctcctc?ttccccgcgc?ggtcaagccg?gacccccccc?acccgccatt 120
aaagccgacc?tccacctctc?ctcctcctcc?tcctttgggt?ctccctgggg?cctatttaag 180
cgagcaccgt?g 191
<210>56
<211>193
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(146)..(157)
<223>
<400>56
tcctagccct?accctgtccc?actttcggtc?acgccgctca?tccccgcctc?cgccgcgccc 60
gtgcaccccc?cctttttgcc?ccttcgccgc?gattctcgcc?gcttctcgcg?gcccgccatt 120
aaagccggtg?cgacaacctc?tccccctcct?cctcctctgg?gcctccccga?gccctattta 180
agcgagcacc?gtg 193
<210>57
<211>189
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(142)..(153)
<223>
<400>57
tcctagccct?accctgtccc?cttttcggtc?gcgcagcccg?tcctcgcctc?cgccgcagcc 60
gcgcgccccg?cttttcccac?actccacgcg?gtaaagcctg?cacccgccgc?tcgccattaa 120
agccgacctc?cacctttccc?cctcctcctc?ctccgggtct?ccctggggcc?tatttaagcg 180
agcaccgtg 189
<210>58
<211>117
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>58
tcgcatctaa?acaacatcaa?aacagcgggg?aaatgaattg?atacccttgg?agacgcgtga 60
aacgctgaac?aaaaacccac?gaagatgatc?ttcttcttct?cctcctttct?tcttctc 117
<210>59
<211>117
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>59
tcgcatctaa?acaacatcaa?aacagcgggg?aaatgaattg?atacccttgg?agacgcgtga 60
aacgctgaac?aaaaacccac?gaagatgatc?ttcttcttct?cctcctttct?tcttctc 117
<210>60
<211>117
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>60
tcgcatctaa?acaacatcaa?aacagcgggg?aaacgaattg?atacccttgg?agatgcatga 60
aacgctgaac?aaaaacccac?gaagatgata?ttcttcttct?cctcctttct?tcttctc 117
<210>61
<211>117
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>61
tcgcatctaa?acaacatcaa?aacagcgggg?aaacgaatgg?atacccttgg?agacgcgtga 60
aacgctgaac?aaaaacccat?gaagatgatc?ttcttcttct?cctcctttct?tcttctc 117
<210>62
<211>117
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>62
tcgcatctaa?acaacatcaa?aacagcgggg?aaacaaattg?atacccttgg?agatgcgtga 60
aacgctgaac?aaaaacccac?gaagatgatc?ttcttcttct?cctcctttct?tcttctc 117
<210>63
<211>117
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(89)..(100)
<223>
<400>63
tcgcatctaa?acaacatcaa?aacagcgggg?aaataaattg?atacccttgg?agatgcgtga 60
aacgctgaac?aaaaacccac?gaagatgatc?ttcttcttct?cctcctttct?tcttctc 117
<210>64
<211>301
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(147)..(155)
<223>
<400>64
agggtgttat?ttgctattgt?ccaaccttgc?cacatgttat?atattggcct?ttggcccatt 60
ggaatagatc?agatgtatta?ttctaacatg?gtatcagagc?catggaaacc?ctaatcatag 120
aaaccctacc?tccttgacca?acaaccgccg?ccgccactgc?ccccgcgcca?agcctcctac 180
tgctcttctc?ttccttggaa?ccggtctaag?ccacccgagt?gccgcctcca?tccgagcagc 240
tgaggtcgtg?cggcgcctct?acctgagcgc?cgcctccacg?tcggcctcca?gcgtcggatt 300
t 301
<210>65
<211>279
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(140)..(151)
<223>
<400>65
agggtgttat?ttgctattgt?ccaaccttgt?acatattata?tattggcctt?tggcccattg 60
gaatagacaa?gatgctttat?tccaaacatg?gtatcagagc?catagaaacc?ctaaccatga 120
aaaccctacc?tcttgaccag?ccgccgccgc?cgctgtcctg?ttgctcctct?ccccccagat 180
ccgcgcctga?tttttggtgt?cgagctctta?ccgtgccggc?ctccttctcc?gtcggccttc 240
atccgagcag?gcccgagcgt?cgcctccagc?gtcggattt 279
<210>66
<211>264
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(160)..(171)
<223>
<400>66
agggtgttat?ttgctattgt?gtttaacctt?gtacatgtta?tatattggcc?tttggcccat 60
tagaatacac?aagatgcttt?attccaaata?tggtatcaga?gctatagaaa?ccctagccat 120
agaaacccta?cctcttagcc?aacagctgct?accactgtag?ccgccgccgc?cattgcacat 180
cagcgtgcgg?ccgcgcgcgc?ccacaccagc?ctctgctcac?ggctcctctc?cgccgcctcc 240
acatcggcct?ccagcgtcgg?attt 264
<210>67
<211>331
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(145)..(153)
<223>
<400>67
agggtgttat?ttgctattgt?ccaatcttgt?acatgttata?tactagcctt?tgacctattg 60
gaatagagac?gatgcattat?tccaactggt?atcggagtca?tggaaaccct?agctatagaa 120
accctacctc?cttgaccaac?caccgccgcc?gccaccatcc?ctgcgccgag?cttcttcccg 180
ctgctcttct?ctctctcgga?ttcgtgccaa?cccgagcgcc?gcctcctcaa?ctccagccag 240
cgtttgctcc?gcgcctctag?cgacgcctcc?acgccggcct?cccgcgccag?attcacaccg 300
gcgcctgctc?catgcctcca?gcgtcggatt?t 331
<210>68
<211>146
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(77)..(92)
<223>
<400>68
aaagatgttg?ccttgttcat?aaatgttgta?tgttcaatta?ttcactgtac?ttgctgagtc 60
tcggactcat?tcttgctttg?tttgtttgtt?tgtttatttt?aggtgatcgt?tgatgatggc 120
tctattggga?gggaatagac?atgcac 146
<210>69
<211>146
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(77)..(92)
<223>
<400>69
aaagatgttg?ccttgttcat?aattgttgta?tgttcaatta?ttcactgtac?ttgctgagtc 60
tcggactcat?tcttgctttg?tttgtttgtt?tgtttatttt?aggtgatcgt?tgatgagggc 120
tctattggga?gggaatagac?atgcac 146
<210>70
<211>146
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(77)..(92)
<223>
<400>70
aaagatgttg?ccttgttcat?aatggtcgta?tgctcaatta?ttcactgtac?ttgctgagtc 60
tcggactcat?tcttgctttg?tttgtttgtt?tgtttatttt?aggtgatcgt?tgatgatggc 120
tctattggga?gggaatagac?atgcac 146
<210>71
<211>146
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(77)..(92)
<223>
<400>71
aaagatgttg?ccttgttcat?aattgttgta?agttcaatta?ttcactgtac?ttgctgagtc 60
tcggactcat?tcttgctttg?tttgtttgtt?tgtttatttt?aggtgatcgt?tgacgacggc 120
tctattggga?gggaatagac?atgcac 146
<210>72
<211>146
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(77)..(92)
<223>
<400>72
aaagatgttg?ccttgttcat?aattgttgta?agttcaatta?ttcattgtac?ttgctgagtc 60
tcgaactcat?tcttgctttg?tttgtttgtt?tgtttatttt?aggtgatcgt?tgatgatggc 120
tctattggga?gggaatagac?atgcac 146
<210>73
<211>138
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(77)..(88)
<223>
<400>73
aaagatgttg?ccttgttcat?aattgttgta?tgttcaatta?ttcactgtac?ttgctaagtc 60
tcggactcat?tcttgctttg?tttgtttgtt?ttaggtgatc?gttgatgatg?gctctattgg 120
gagggaatag?acatgcac 138
<210>74
<211>208
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>74
tttcatggag?ggcgacaggg?tgctcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcacttgct?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>75
<211>208
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>75
tttcatggag?ggcgacaggg?tgctcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcacttgcc?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>76
<211>208
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>76
tttcatggag?ggcgacaggg?tgctcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcacttgct?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>77
<211>208
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>77
tttcatggag?ggcgacaggg?tgctcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcacttgct?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>78
<211>208
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>78
tttcatggag?ggcgacaggg?tgctcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcacttgct?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>79
<211>208
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(98)..(112)
<223>
<400>79
tttcatggag?ggcgacaggg?aactcgccta?ccacggcccc?ctcctctacg?aggccaaggt 60
tccctttctc?ctcactggaa?tcctccgttc?aattcaattc?cgttccgttc?cgttccaatc 120
tagggttatt?attacatgta?ttggcattcc?cctccggatg?gccatgtcga?atcgattctt 180
cctagctttc?gtttgtttcc?tggtcggc 208
<210>80
<211>281
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>80
gttgtgccca?gcactttaga?agaaggttag?caggggcagg?caaccaatta?gagggattga 60
gagagaatag?agattgaata?aaataactaa?attgcatgac?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?gttttttgta?ggagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttgat?tacgctatat?aaattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>81
<211>281
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>81
gttgtgccca?gcactttaga?agaaggttag?caagggcagg?ctacccctta?gagggattgg 60
aagagaatag?agattgaata?aaataaccaa?attgcatgat?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?gttttttgta?ggagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttgat?tacgctatat?taattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>82
<211>281
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>82
gttgtgccca?gcactttaga?agaaggttag?caggggcagg?caaagaatta?gagggattgg 60
gagagaatag?agattgaata?aaataactaa?attgcatgat?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?gttttttgta?ggagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttgat?tacgctatat?taattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>83
<211>281
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>83
gttgtgccca?gcactttaga?agaaggttag?caggggcagg?caaccaatta?gagggattgg 60
gagagaatag?agattgaata?aaataactaa?attgcatgat?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?gttttttgta?ggagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttgat?tacgctatat?taattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>84
<211>281
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>84
gttgtgccca?gcactttaga?agaaggttag?caagggcagg?caaccaatta?gagggattgg 60
gagagaatag?agattgaata?aaatagctaa?attgcatgat?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?gttttttgta?ggagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttgat?tacgctatat?taattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>85
<211>281
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(231)..(255)
<223>
<400>85
gttgtgccca?gcactttaga?agaaggttag?caggggtagg?caaagaatta?gagggattgg 60
gagagaatag?agattgaata?aaataactaa?attgcatgat?tttctaagca?tgatcttgta 120
tgtcttccat?ttatttgctg?catgttttta?attttttgta?tgagcttact?tgattacgtt 180
atattacatt?tttctatgag?cttacttggt?tacgctatat?taattgattt?atttcatttc 240
atttcatttc?atttcaggac?accaagaaat?gagtggcgtc?a 281
<210>86
<211>144
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(95)..(114)
<223>
<400>86
ctatcatcaa?atgtgcctca?atatttatgt?gaattttaaa?attcaaacca?ccttcccatt 60
tttctaatca?cggctggggg?tggataacga?gccagctcgg?ctcggctcgg?ctcgttaagc 120
taatgagcag?aaagtgaggc?tcgg 144
<210>87
<211>268
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(248)..(265)
<223>
<400>87
aaagcaacca?cgccattaat?atactcttgg?ctcacacggt?tcataccgta?catccagttt 60
cggtccatct?atgatacgca?tgtaacaaat?tagtgtcaaa?ttagattaat?taatcacaac 120
tacgtactca?tcctatttac?caaacatact?taaattgatt?agctattaat?ttattgcaaa 180
cataaagggt?tatagatcgt?taccctcaga?gctcacgacg?aaaataccaa?atatatcttg 240
agaatcctct?ccttctcctt?ctcctccg 268
<210>88
<211>281
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(259)..(276)
<223>
<400>88
aaagcaacca?cgccattaat?gtactcttga?ctcacacggc?tcacaccgta?catccagttt 60
tggtccatct?atacgcatgt?aacaaatcag?tgtcaaatta?cattaataaa?ccacaagtac 120
aaacccttct?taattattga?ccaaacatac?ttaaattgct?tagcgattaa?cctagagcaa 180
atataaaggg?ttaaagatcg?ttaccctagg?agctcacgac?aaaygggcca?aatatcctct 240
gagatcctcg?ggaatccttt?ctccttctcc?ttctcctccg?a 281
<210>89
<211>280
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(248)..(277)
<223>
<400>89
aaagcaacca?cgccattaat?gtactcttga?ctcccacagt?tcacaccgta?catccagttt 60
tagtccatct?acgatacaca?tgtaacaaat?tagtgtcaaa?ttagattaat?taaccgcaag 120
tgcgtacttt?ttctatttac?caaacatact?taaattgatt?agagattaat?ttatagcaaa 180
tataaagggt?tatagatcgt?taccctcaga?gctcatgatg?aaaataccaa?atatctctcg 240
agaatcctct?ccttctcctt?ctccttctcc?ttctcctccg 280
<210>90
<211>285
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(253)..(261)
<223>
<400>90
aaagcaacca?cgccattaat?atactcttga?ctcacacggt?tcacaccgta?catccaattt 60
cggtccatct?acactacaca?tgcaacaaat?taattagtgt?caaattagat?taataaacca 120
caagtacgta?ctcttcctat?tgaccaaaca?tacttatatt?gattagcgat?taacttatag 180
caaacataaa?gggttataga?ttgttaccct?tagagctcat?gacgaaaata?ctaaatatcc 240
ctcgagattc?ctctcctcct?ccttctcctt?ctccttctcc?tccga 285
<210>91
<211>271
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(250)..(267)
<223>
<400>91
aaagcaacca?cgccattaat?gtactcttga?ctcacacagc?tctcaccgta?catccagttt 60
tggtccatct?atacgcatgt?aacaaatcag?tgtcaaatta?cattaataac?ccgcaagtac 120
aaactcttct?taattattga?ccaaacatac?ttaaattgct?taacgatata?acctagagca 180
aatataaagg?gttaaagatc?gttaccctcg?gagctcacga?caaaaatacc?aaatatcccg 240
cgagattcct?ctccttctcc?ttctcctccg?a 271
<210>92
<211>258
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(237)..(254)
<223>
<400>92
aaagcaacca?cgccattaat?gtactcttga?ctcacacgat?tcacaccgta?aatccagttt 60
cggtccatct?acgctacaca?tgtaacaaat?tagattaata?aaccgcaagt?acgtactctt 120
cctatttacc?aaacatactt?aaattgatta?gcgattaatt?tatagcaaac?ataaagggtt 180
atagatcgtt?accctcgaag?ctcacgacga?aaatactaaa?tatctctcga?gaatcctctc 240
cttctccttc?tcctccga 258
<210>93
<211>157
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(102)..(121)
<223>
<400>93
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagccgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>94
<211>157
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(108)..(122)
<223>
<400>94
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagctgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>95
<211>157
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(102)..(121)
<223>
<400>95
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagccgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>96
<211>157
<212>DNA
<213>Phyllostachys?praecox
<220>
<221>repeat_region
<222>(108)..(122)
<223>
<400>96
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagctgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>97
<211>157
<212>DNA
<213>Phyllostachys?nidularia
<220>
<221>repeat_region
<222>(102)..(121)
<223>
<400>97
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagccgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>98
<211>157
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(102)..(121)
<223>
<400>98
caggtagtcg?tcttatcgag?tttttcttga?aattgacata?gtttgattta?atatcatttt 60
atttatagtt?tgaaagtatt?tttatgaccg?agctaatgag?ccgagctgag?ccgagccgag 120
ccagggtgaa?ttccaatttc?attcgaaatt?gacttat 157
<210>99
<211>374
<212>DNA
<213>Phyllostachys?heteroclada
<220>
<221>repeat_region
<222>(122)..(127)
<223>
<220>
<221>repeat_region
<222>(165)..(179)
<223>
<220>
<221>repeat_region
<222>(208)..(213)
<223>
<400>99
gttgcccgtg?gttactgtct?catccttctc?atgctgccgg?ttttaatggc?acaagcagcc 60
actgcttggg?gctttataga?gaaaagttat?cacaggcggt?ttcagccaca?aaccgtctat 120
gatatatact?tatcacagac?gattttagcc?acaaaccgtc?tgtgatatat?acttatcaca 180
gacggttgca?gccacaaacc?gtctatgata?tatacttatc?gcagacgatt?tcagccacaa 240
accgtctgtg?ataggttcct?atcacagacg?ttttcagtta?ataaccgtct?gtgagagtat 300
gcatcacaga?cggtttgaaa?actgtctatg?atgacctcat?gtctttactg?acccataaac 360
tcagatggct?cacg 374
<210>100
<211>255
<212>DNA
<213>Phyllostachys?bambusoides
<220>
<221>repeat_region
<222>(62)..(67)
<223>
<220>
<221>repeat_region
<222>(77)..(86)
<223>
<400>100
gttgcccgtg?gttactgtct?catccttctc?atgctgcgtg?gacgatcccc?ccaaagtggc 60
tgagagacta?cgaacaatat?atatatgatg?ccggatttaa?tggcacaagc?agccactgct 120
tagggcttta?tagagaaaag?tcatcacaga?cggtttgtgg?ctgaaaccgt?ctgtgaaagt 180
atgcatcaca?gacggttcaa?aactatctgt?gatgacctca?tgtcatcact?gacccataaa 240
ctcagatggc?tcacg 255
<210>101
<211>299
<212>DNA
<213>Phyllostachys?atrovaginata
<220>
<221>repeat_region
<222>(62)..(67)
<223>
<220>
<221>repeat_region
<222>(81)..(86)
<223>
<400>101
gttgcccgtg?gttactgtct?catccttctc?atgctgcgtg?gatgattccc?ccaaagtggt 60
tgagagacta?cgaacaatac?atatatgatg?ccggttttaa?tggcacaagc?agccactgct 120
tggggcttta?tagagaaaag?tcatcacaga?agatttcagc?cacaaaccgt?ctatgatagg 180
ttcctatcat?agacggtttc?agttaataac?cgtctgtaaa?agtatgcatc?acagacggtt 240
tgaaaaccgt?ctgtgatgac?ctcatgtcat?cactgaccca?taaactcaga?tggctcacg 299
<210>102
<211>220
<212>DNA
<213>Phyllostachys?kwangsiensis
<220>
<221>repeat_region
<222>(62)..(71)
<223>
<400>102
gttgcccgtg?gttactgtct?catcctcaca?atcgcccgaa?agtgactagg?ggacatgaac 60
aatatatata?tgaagccagt?tttaaaggca?caagcaacca?ctgcttggtc?agtttataaa 120
gaatactatc?acagacagtt?tcatccacaa?actgtttgtg?atagtatgca?tctgtgatga 180
tctcatgtca?ttactgatct?ataaactcag?atggctcacg 220
Claims (4)
1, a kind of development approach of moso bamboo microsatellite molecular marker, its feature comprises the steps:
(1) acquisition of mao bamboon dna sequence dna: the Latin formal name used at school of using mao bamboon is as keyword, and retrieval and download obtain in the geneseq database that NIH safeguards;
(2) contain the screening of microsatellite DNA sequence: with little satellite routine analyzer of standalone version and online microsatellite locus analysis software, the sequence of downloading is screened, obtain to contain the mao bamboon dna sequence dna of microsatellite locus;
(3) design of primers and microsatellite locus checking:
1. design of primers: contain the archaeal dna polymerase chain reaction primer of microsatellite locus with primer-design software design amplification, primer contains the base numerical control built in 18-22, two primer annealing temperature head＜4 ℃, and the dna fragmentation of amplification is in 350 bases;
2. little satellite checking: utilize above-mentioned primer to carry out amplification again, subclone and order-checking affirmation work that detection, microsatellite locus are dyed in archaeal dna polymerase chain reaction amplification, the electrophoretic separation of amplified production, silver, obtain 19 pairs of moso bamboo microsatellite labeled primers at last, its annealing temperature is at 46 ℃-59 ℃.
2, the development approach with the described moso bamboo microsatellite molecular marker of claim 1 is characterized in that the primer that obtains 19 pairs of moso bamboo microsatellite marks is respectively:
PBM002：5’-ATGGAGCATTCATTTGAG-3’ 5’-TTGACAAGTTTGGAGGGT-3’
PBM004：5’-CGATGAAGTTGCCTGTGG-3’ 5’-TCAAGTCGCATTGGAGTA-3’
PBM007：5’-CTCGAGGTGAAGTTACATTA-3’ 5’-TCGTAGAGCACTATCCATTT-3’
PBM011：5’-TAAAGAAGTATAATGTATTCG-3’?5’-AGCTGTTTTAGCATCTATGTT-3’
PBM013：5’-TTTTACTAACGTGCCCGTATT-3’?5’-GGAGGATCGCTGGATTGG-3’
PBM014：5’-GCCGTCCAAACGCTCCTT-3’ 5’-CACCCATCCATCTTATGCTAT-3’
PBM016：5’-CCCACAGAAGGTCGCACTA-3’ 5’-CCGCCGAGAACGTCTACAT-3’
PBM017：5’-TATGCCTCCAATAATCCG-3’ 5’-AAGGCGTAGCCACCGAAT-3’
PBM018：5’-GACGACGAGTGTAACGCTGAG-3’?5’-AGGATGATGCCGGTGAGG-3’
PBM019：5’-TCCTAGCCCTACCCTGTCC-3’ 5’-CACGGTGCTCGCTTAAATAG-3’
PBM020：5’-TCGCATCTAAACAACATC-3’ 5’-GAGAAGAAGAAAGGAGGA-3’
PBM021：5’-AGGGTGTTATTTGCTATTGT-3’ 5’-AAATCCGACGCTGGAGGC-3’
PBM022：5’-AAAGATGTTGCCTTGTTC-3’ 5’-GTGCATGTCTATTCCCTC-3’
PBM023：5’-TTTCATGGAGGGCGACAG-3’ 5’-GCCGACCAGGAAACAAACGAA-3’
PBM025：5’-GTTGTGCCCAGCACTTTA-3’ 5’-TGACGCCACTCATTTCTT-3’
PBM026：5’-CTATCATCAAATGTGCCTCAA-3’5’-CCGAGCCTCACTTTCTGC-3’
PBM027：5’-AAAGCAACCACGCCATTA-3’ 5’-TCGGAGGAGAAGGAGAAG-3’
PBM028：5’-CAGGTAGTCGTCTTATCGAGT-3’5’-ATAAGTCAATTTCGAATGAA-3’
PBM030：5’-GTTGCCCGTGGTTACTGT-3’ 5’-CGTGAGCCATCTGAGTTT-3’
3, as the development approach of claim 2 described moso bamboo microsatellite molecular markers, it is characterized in that numbering is respectively PBM002, PBM004, PBM007, PBM011, PBM013, PBM014, PBM016, PBM017, PBM018, PBM019, PBM020, PBM021, PBM022, PBM023, PBM025, PBM026, PBM027, PBM028, the annealing temperature of the primer at little satellite nucleotide sequence two ends of PBM030 is respectively 48 ℃, 48 ℃, 53 ℃, 50 ℃, 53 ℃, 52 ℃, 56 ℃, 55 ℃, 52 ℃, 59 ℃, 46 ℃, 55 ℃, 47 ℃, 58 ℃, 47 ℃, 50 ℃, 49 ℃, 53 ℃, 50 ℃.
4, the application of moso bamboo microsatellite molecular marker as claimed in claim 2, it is characterized in that said primer, be applied to an ancient plucked stringed instrument bamboo, black bud bamboo, Lei Zhu, fishscale bamboo, false mao bamboon and 6 Phyllostachys of phyllostachys bambusoides and represent the exploitation of the little satellite of bamboo kind, confirm that by amplification and order-checking having obtained 102 Phyllostachys dna sequence dnas that contain microsatellite locus sees Table (2).
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| CN200910102125A CN101629196A (en) | 2009-08-13 | 2009-08-13 | Development and application of moso bamboo microsatellite molecular marker |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533683A (en) * | 2010-12-14 | 2012-07-04 | 国际竹藤网络中心 | Phyllostachys edulis violaxanthin deepoxidase (PeVDE) protein, and coding gene and applications thereof |
| CN103233066A (en) * | 2013-04-17 | 2013-08-07 | 杨汉奇 | Molecular marker identification method for different provenance types of dendrocalamus sinicus |
| CN112430683A (en) * | 2020-12-10 | 2021-03-02 | 山东省林业科学研究院 | SSR molecular marker related to cold resistance of bamboo, SSR primer and application of SSR molecular marker |
| CN112662806A (en) * | 2021-01-29 | 2021-04-16 | 广东省农业科学院环境园艺研究所 | Rhynchosia SSR molecular marker primer composition and application thereof |
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2009
- 2009-08-13 CN CN200910102125A patent/CN101629196A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533683A (en) * | 2010-12-14 | 2012-07-04 | 国际竹藤网络中心 | Phyllostachys edulis violaxanthin deepoxidase (PeVDE) protein, and coding gene and applications thereof |
| CN102533683B (en) * | 2010-12-14 | 2013-05-22 | 国际竹藤中心 | Phyllostachys edulis violaxanthin deepoxidase (PeVDE) protein, coding gene thereof and applications thereof |
| CN103233066A (en) * | 2013-04-17 | 2013-08-07 | 杨汉奇 | Molecular marker identification method for different provenance types of dendrocalamus sinicus |
| CN112430683A (en) * | 2020-12-10 | 2021-03-02 | 山东省林业科学研究院 | SSR molecular marker related to cold resistance of bamboo, SSR primer and application of SSR molecular marker |
| CN112662806A (en) * | 2021-01-29 | 2021-04-16 | 广东省农业科学院环境园艺研究所 | Rhynchosia SSR molecular marker primer composition and application thereof |
| CN112662806B (en) * | 2021-01-29 | 2021-07-30 | 广东省农业科学院环境园艺研究所 | Rhynchosia SSR molecular marker primer composition and application thereof |
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