CN101627723B - Method for obtaining male sterile line of cabbage - Google Patents
Method for obtaining male sterile line of cabbage Download PDFInfo
- Publication number
- CN101627723B CN101627723B CN200810116694XA CN200810116694A CN101627723B CN 101627723 B CN101627723 B CN 101627723B CN 200810116694X A CN200810116694X A CN 200810116694XA CN 200810116694 A CN200810116694 A CN 200810116694A CN 101627723 B CN101627723 B CN 101627723B
- Authority
- CN
- China
- Prior art keywords
- cabbage
- male sterile
- sterile line
- male
- parent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention discloses a method for obtaining a male sterile line of cabbage, which comprises the following steps that: (1) Heterologous Brassica hexaploid (AABBCC, n=27) Qiuyin 2005-12 is used as a female parent and a spring seed cabbage Jinchun 2005-12 containing an A gene group (AA, n=10) is used as a male parent to obtain hybrid generation I; a preservation and registration number of the heterologous Brassica hexaploid (AABBCC, n=27) Qiuyin 2005-12 is CGMCC No 2553; and (2) mid-late ripe cabbage Yihe No 5 containing the A gene group (AA, n=10) is used as a female parent and the hybrid generation I obtained in the step one is used as a male parent for hybridization to obtain the male sterile line of the cabbage. The experiment proves that through two repeated experiments adopting the method, the result can obtain the male sterile line of the cabbage every time; in 11 plants of the cabbage, 11 plants are sterile; the sterile plant rate is 100 percent; and the sterility is 100 percent.
Description
Technical field
The present invention relates to a kind of method that obtains male sterile line of cabbage.
Background technology
Plant can not produce normal flower pesticide, pollen or andro gamete in sexual reproduction process genetic phenomenon is called male sterile (male sterility).According to genetic characteristics, male sterile can be divided into two classes: promptly meet mendelian inheritance, nuclear male sterility (nuclear male sterility by nuclear gene control, NMS or genic malesterility, GMS) with by the nucleo-cytoplasmic interreaction male sterility of the common control of cell nucleus, cytoplasmic inheritance factor, also claim cytoplasmic male sterility (cytoplasmic male sterility, CMS).Male sterile is the instrument of crop heterosis utilization, recurrent selection and population improvement, is again the important genetic stocks of research pollen development, cytoplasmic inheritance and nucleo-cytoplasmic interaction.
At present, Chinese cabbage male sterile source mainly contains two kinds of genie male sterile line and cytoplasmic male sterile lines, be respectively the genie male sterile line that Zhang Shufang etc. (1990) finds from " the ten thousand spring Qing Gangs " Chinese cabbage cultivar seed collecting Tanaka, and the transformation rape belong to Ogu CMS, Pol CMS and three kinds of cytoplasmic male sterile lines of CMS96 that other crop cell matter male sterile lines obtain.The main path that male sterile line of cabbage obtains has following several:
1, spontaneous mutation
Mostly original sterile source is that the natural mutant strain found, spontaneous mutation are the main paties that sterile source produces from natural population.Nineteen ninety Zhang Shufang etc. has found sterile strain from " the ten thousand spring Qing Gangs " Chinese cabbage cultivar seed collecting Tanaka, and the sterile type of this sterile strain is a nuclear male sterility.
2, hybridization and backcross transformation
Between genus or interspecific cross be to obtain the especially effective way of cytoplasmic male sterile line of male sterile line.According to Kaul (1988) statistics, in the male sterile material of having found, there are nuclear male sterility material about 10% and about 70% cytoplasmic male sterility material to come between genus or interspecific cross.
Cabbage cytoplasm male sterile line derives from radish Ogu CMS, cabbage type rape Pol CMS and these three kinds of sterile sources of novel cabbage type rape C MS96 respectively, the process protoplast merges, hybridizes and how for approach such as backcross transformations, obtained three kinds of cabbage cytoplasm male sterile lines of present application.
Summary of the invention
The purpose of this invention is to provide a kind of method that obtains male sterile line of cabbage.
The method of acquisition male sterile line of cabbage provided by the present invention may further comprise the steps:
1) with rape belong to allohexaploid (AABBCC, n=27) autumn is drawn 2005-12 for maternal, with contain the A genome (AA, spring sowing Chinese cabbage gold spring n=10) is that male parent is hybridized, and obtains first-filial generation; Described rape belong to allohexaploid (AABBCC, n=27) to draw the preservation registration number of 2005-12 be CGMCC № 2553 autumn;
2) (AA, late-maturing Chinese cabbage justice and be for No. five maternal in n=10) are that male parent is hybridized with the first-filial generation of above-mentioned step 1) acquisition, the acquisition male sterile line of cabbage to contain the A genome.
Based on the above method, can also be with above-mentioned steps 2) male sterile line of cabbage that obtains be female parent, adopted and No. five or Beijing are that male parent is hybridized new No. three with Chinese cabbage, the male sterile line of cabbage of acquisition transformation.
Experiment showed, 2006-2007, adopt said method to carry out repeated experiments twice, the result can both obtain male sterile line of cabbage at every turn, in 11 strain Chinese cabbage, has 11 strains sterile, and sterile plant rate is 100%, and sterile degree is 100%.
Rape belongs to allohexaploid (AABBCC, n=27) autumn draws 2005-12 and has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on June 18th, 2008 and (is called for short CGMCC, the address is: the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation registration number is CGMCC № 2553.Rape belongs to allohexaploid (AABBCC, n=27) autumn is drawn the phenotypic character that 2005-12 has following genetic stability: the plant type height, lobus cardiacus is purple slightly, leaf green, incises, adheres to the wax powder, stem slightly purple, adhere to the wax powder, the axillalry bud concurrency is strong, petal is yellow, and flower pesticide and column cap are normal, and fecundity is good, kind of pod is elongated, and seed is big, be pitchy.
The method of acquisition male sterile line of cabbage of the present invention has following advantage: the male sterile line sterility of simple, good reproducibility, acquisition is stable, sterile thoroughly, and transformation is easy, and good with other Chinese cabbage crossings, can be used for the research of association areas such as Chinese cabbage breeding, sterile mechanism.
Description of drawings
Fig. 1 is the concrete technology path of the present invention
Fig. 2 is the form of male sterile line of cabbage MS200712 flower
Fig. 3 is flower pesticide and the nectary of male sterile line of cabbage MS200712
Embodiment
Concrete technology path of the present invention as shown in Figure 1.
The acquisition of embodiment 1, male sterile line of cabbage
1, the first-filial generation F1 (acquisition of AABBCC * AA)
Belong to allohexaploid (AABBCC with rape, n=27) autumn is drawn 2005-12 CGMCC № 2553 for maternal, to contain A genome (AA, n=10) the spring sowing Chinese cabbage gold spring, (available from the international seedling in East Asia, Liaoning Co., Ltd) hybridized for male parent, belong to allohexaploid (AABBCC above-mentioned female parent-rape, n=27) autumn is drawn the full-bloom stage of 2005-12, choose many, the eugonic major branches of bud and be the hybridization branch, thoroughly extract 6 stamens in each bud on the inflorescence, the pollen of smearing male parent-spring sowing Chinese cabbage gold spring then is to the column cap of above-mentioned female parent; The next morning repeats pollination once again.Be first-filial generation F1 (AABBCC * AA) through the seed of gathering in the crops on the female parent of above-mentioned processing.
2, the acquisition of male sterile line of cabbage MS200712
To contain A genome (AA, n=10) late-maturing Chinese cabbage justice and No. five (available from the white vegetable breeding of glue center, Jiaozhou City, Qingdao) are maternal in, (AABBCC * AA) is that male parent is hybridized to the first-filial generation F1 that obtains with step 1, above-mentioned female parent-in the full-bloom stage of late-maturing Chinese cabbage justice and No. five, choose many, the eugonic major branches of bud and be the hybridization branch, thoroughly extract 6 stamens in each bud on the inflorescence, (pollen of AABBCC * AA) is to the column cap of above-mentioned female parent to smear the first-filial generation F1 that above-mentioned steps 1 obtains then; The next morning repeats pollination once again, is being male sterile line of cabbage MS200712 through the seed of gathering in the crops on the female parent of above-mentioned processing.
The form of male sterile line of cabbage MS200712 flower as shown in Figure 2, the form of its flower pesticide and nectary is as shown in Figure 3.As can be seen from the figure, the petal of male sterile line of cabbage MS200712 is yellow, and flower pesticide is light yellow, and flower pesticide is degenerated, abortion is thorough, and column cap is normal, the nectary prosperity.
2006-2007 adopts said method to carry out repeated experiments twice, and the result can both obtain male sterile line of cabbage at every turn.Repeated experiments 1 has 5 strains sterile in 5 strain Chinese cabbage, and sterile plant rate is 100%, and sterile degree is 100%; Repeated experiments 2 has 6 strains sterile in 6 strain Chinese cabbage, and sterile plant rate is 100%, and sterile degree is 100%.
Embodiment 2, male sterile line of cabbage MS200712
08-840And MS200712
08-916Acquisition
The male sterile line of cabbage MS200712 that obtains with the foregoing description 1 is a female parent, be that male parent is hybridized with Chinese cabbage justice and No. five or Beijing new No. three (respectively available from glue white vegetable breeding center, Jiaozhou City, Qingdao and Beijing Jingyanyinong Technology Development Center) respectively, full-bloom stage at above-mentioned female parent-male sterile line of cabbage MS200712, choose many, the eugonic major branches of bud and be the hybridization branch, artificial strip off bud exposes column cap, smears new No. three pollen in Chinese cabbage justice and No. five or Beijing then respectively to the column cap of above-mentioned female parent; The next morning repeats pollination once again, is being male sterile line of cabbage MS200712 through the seed of gathering in the crops on the female parent of above-mentioned processing
08-840And MS200712
08-916
Adopt said method to carry out repeated experiments twice, the result can both obtain male sterile line of cabbage at every turn.Repeated experiments 1 has 3 strains sterile in 3 strain Chinese cabbage, and sterile plant rate is 100%, and sterile degree is 100%; Repeated experiments 2 has 5 strains sterile in 5 strain Chinese cabbage, and sterile plant rate is 100%, and sterile degree is 100%.
Claims (3)
1. method that obtains male sterile line of cabbage may further comprise the steps:
1) with rape belong to allohexaploid (AABBCC, n=27) autumn is drawn 2005-12 for maternal, with contain the A genome (AA, spring sowing Chinese cabbage gold spring n=10) is that male parent is hybridized, and obtains first-filial generation; Described rape belong to allohexaploid (AABBCC, n=27) to draw the preservation registration number of 2005-12 be CGMCC № 2553 autumn;
2) (AA, late-maturing Chinese cabbage justice and be for No. five maternal in n=10) are that male parent is hybridized with the first-filial generation of above-mentioned step 1) acquisition, the acquisition male sterile line of cabbage to contain the A genome.
2. method that obtains male sterile line of cabbage, be to be female parent with the male sterile line of cabbage that obtains in accordance with the method for claim 1, with in late-maturing Chinese cabbage justice and No. five or Beijing be that male parent is hybridized new No. three, the male sterile line of cabbage of acquisition transformation.
3. claim 1 or 2 application of described method in the Chinese cabbage breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810116694XA CN101627723B (en) | 2008-07-15 | 2008-07-15 | Method for obtaining male sterile line of cabbage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810116694XA CN101627723B (en) | 2008-07-15 | 2008-07-15 | Method for obtaining male sterile line of cabbage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101627723A CN101627723A (en) | 2010-01-20 |
| CN101627723B true CN101627723B (en) | 2011-07-13 |
Family
ID=41573131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810116694XA Expired - Fee Related CN101627723B (en) | 2008-07-15 | 2008-07-15 | Method for obtaining male sterile line of cabbage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101627723B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919117B (en) * | 2012-11-14 | 2014-07-16 | 中国农业科学院蔬菜花卉研究所 | Method for quickly transforming cytoplasmic male sterile line of brassica vegetables |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1849881A (en) * | 2006-06-05 | 2006-10-25 | 华中农业大学 | Selective breeding method for cabbage cytoplasm male sterile line |
| CN1895029A (en) * | 2006-06-15 | 2007-01-17 | 西北农林科技大学 | Breeding method of cabbage plasmic male sterile system materials |
-
2008
- 2008-07-15 CN CN200810116694XA patent/CN101627723B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1849881A (en) * | 2006-06-05 | 2006-10-25 | 华中农业大学 | Selective breeding method for cabbage cytoplasm male sterile line |
| CN1895029A (en) * | 2006-06-15 | 2007-01-17 | 西北农林科技大学 | Breeding method of cabbage plasmic male sterile system materials |
Non-Patent Citations (1)
| Title |
|---|
| 周邦福.天津型大白菜雄性不育系的选育及利用.《湖南农业大学学报(自然科学版)》.2008,第34卷(第2期),190-192. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101627723A (en) | 2010-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lee et al. | Developing stable progenies of× Brassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture | |
| CN104285776B (en) | The selection of male sterile line of maize | |
| CN109349099A (en) | Brassica napus recessive gms line is close, distant hybridization selectively breeding hybrid rape selection | |
| Cai et al. | Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars | |
| CN106900536B (en) | Method for directionally transforming rice photo-thermo sensitive genic male sterile line into wide-range cytoplasmic male sterile line | |
| CN104429919B (en) | A kind of method of cabbage type rape abnormity blade new restoring system initiative | |
| Ram et al. | Developing bitter gourd (Momordica charantia L.) populations with a very high proportion of pistillate flowers | |
| CN101627723B (en) | Method for obtaining male sterile line of cabbage | |
| CN111528100B (en) | Method for obtaining distant hybridization progeny of broccoli and cabbage type rape | |
| CN104351037A (en) | Seed production method for NK718 three-line support hybrid seed | |
| Bang et al. | Production of intergeneric hybrids between the C3-C4 intermediate species Diplotaxis tenuifolia (L.) DC. and Raphanus sativus L. | |
| CN108935068B (en) | Method for improving fertility temperature sensitivity of brassica napus pol CMS sterile line | |
| CN107660462A (en) | A kind of hot pepper cytoplasmic male sterile line initiative and three systems while supporting method | |
| CN104351036B (en) | Capital section 968 three series mating hybrid seed production method | |
| CN114027188A (en) | Culture medium for obtaining iris interspecific hybridization progeny by utilizing immature embryos and application of culture medium | |
| Nguyen et al. | Development and molecular characterization of a doubled haploid population derived from a hybrid between japonica rice and wide compatible indica rice | |
| CN107484655B (en) | A kind of selection of anti-clubroot preserved sichuan pickle cytoplasm male sterility hybrid | |
| Anhani | Development of cytoplasmic‐genic male sterility in safflower | |
| CN111512959A (en) | Hybrid rape breeding method capable of early identifying seed purity | |
| CN108925422A (en) | Improve the cytoplasmic male sterility Hybrid preparation method of rapeseed cultivation purity | |
| CN101023735A (en) | Method for culturing soy-beam with stem exposed | |
| Mii | Breeding of ornamental plants through interspecific hybridization using advanced techniques with a special focus on Dianthus, Primula, Cosmos and Kalanchoe | |
| CN104521738B (en) | Jing Nongke 728 three series mating hybrid seed production method | |
| CN110226512B (en) | Application of wheat male sterile line NWMS1 in construction of wheat near-isogenic line and recurrent improvement population | |
| Saraswathi | Morphological and cytogenetic studies in wild species of Cymbopogon martinii (Roxb.) Wats from Kodaicanal (TN) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING JINGYAN YI NONG SCI-TECH DEVELOPMENT CENTE Free format text: FORMER OWNER: BEIJING AGRICULTURE AND FORESTRY ACADEMY OF SCIENCE Effective date: 20120508 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20120508 Address after: 100097, No. 15, middle garden, Shuguang garden, west gate, Haidian District, Beijing Patentee after: Beijing JingYan YiNong Sci-Tech Development Center Address before: 100097 vegetable garden Research Center, Beijing Academy of agriculture and Forestry Sciences, Haidian District, Beijing Patentee before: Beijing Academy of Agriculture and Forestry Sciences |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110713 Termination date: 20150715 |
|
| EXPY | Termination of patent right or utility model |