CN101619329A - Technical method for preparing biodiesel by biocatalysis one-pot method - Google Patents
Technical method for preparing biodiesel by biocatalysis one-pot method Download PDFInfo
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- CN101619329A CN101619329A CN200910055586A CN200910055586A CN101619329A CN 101619329 A CN101619329 A CN 101619329A CN 200910055586 A CN200910055586 A CN 200910055586A CN 200910055586 A CN200910055586 A CN 200910055586A CN 101619329 A CN101619329 A CN 101619329A
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- grease
- biocatalysis
- preparing biodiesel
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- biological catalyst
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention relates to a technical method for preparing biodiesel by a biocatalysis one-pot method. After a grease-contained raw material is crushed, a proper solvent which can not only be used as a grease extracting solvent, but also be used as a grease trans-esterification or esterified acyl receptor and a biocatalyst are added to carry out reaction so as to obtain biodiesel, preferably, the proper solvent is short chain carbonic ester and/or short chain acetic ester, and 3 ml-30 ml of proper solvent is added into the grease-contained raw material per g; the biocatalyst is lipase or a lipase-contained raw material derived from animals, plants or microbes, the adding content of the biocatalyst is 3 percent-30 percent of the theoretical oil content weight of the grease-contained raw material, the reaction temperature is 15 DEG C-65 DEG C, the reaction time is 4 hours-24 hours; and the grease-contained raw material is derived from one or several plants, animals, microbes, algae and other grease-contained industrial wastes or residuals. The invention has skillful design as well as simple and convenient operation and can rapidly prepare the biodiesel, thereby greatly improving the production efficiency, reducing the production cost and being suitable for large-scale industrialized application.
Description
Technical field
The present invention relates to oil chemistry engineering, biochemical engineering, bioenergy technical field, particularly biocatalysis prepares the biodiesel technology field, specifically is meant a kind of processing method of preparing biodiesel by biocatalysis one-pot method.
Background technology
The energy dilemma that the petroleum resources shortage causes with and excessively utilize the ecocrisis that causes to cause the common concern of countries in the world, seek the most important thing that the reproducible environmental protection energy has become various countries' energy strategy.Biofuel is a kind of green regenerative energy sources, and it is the fatty acid alkyl ester that glyceride stock is made by esterification or transesterificationization under the situation that catalyzer exists.The combustionproperty of biofuel can compare favourably with traditional petroleum base diesel oil, and objectionable impurities has reduced by 50% than traditional petrifaction diesel in the tail gas that its burning rear engine gives off.Therefore, biofuel is all being greatly developed in countries in the world.
Biofuel is that transesterification reaction by acid or base catalysis prepares traditionally, yet chemical method complicated process of preparation, equipment requirements height, reaction conditions are difficult to control, alcohol must be excessive; Subsequent technique must have corresponding pure retrieving arrangement, energy consumption height; Color and luster is dark, and unsaturated fatty acids is at high temperature apt to deteriorate in the fat; Esterification products is difficult to reclaim the cost height; Production process has discharging of waste liquid, and environmental pollution is serious.For addressing the above problem, people begin sight turned to and use the biological catalysis biodiesel synthesis, promptly carry out esterification with lipase as catalyzer or transesterification reaction prepares biofuel.Yet, biological catalysis also has problems: on the one hand, when preparing, traditional enzyme process all use short chain alcohol as acyl acceptor, thereby poison biological catalyst lipase but the very low meeting of short chain alcohol such as methyl alcohol solubleness in nonaqueous phase exists with the form of small droplets, cause the vigor loss of lipase and the decline of stability; On the other hand, the domestic and international research report at present uses refining grease as raw material, as soybean oil, sunflower seed oil, rapeseed oil, Oleum Gossypii semen, Thistle oil, castor-oil plant wet goods, therefore the cost of glyceride stock just accounts for more than 70% in the preparation cost of biofuel, thereby causes the very high heavy industrialization that limited of cost of whole technology.
Investigate whole biodiesel manufacture technological process, whether be necessary earlier grease to be extracted that make with extra care the back be an individual considerable problem as the biodiesel manufacture raw material, can screen suitable solvent fully and carry out " one kettle way " preparation biofuel, simplify whole process flow to reach the purpose that reduces the technology cost thereby can dispense greasy extraction and purification step like this.
Carbonates can be used as preparation (Su, E.Z., Zhang, M.J., the Zhang that acyl acceptor is used for biofuel; J.G., Gao, J.F., Wei; D.Z.2007.Biochem.Eng.J.36,167-173), the transesterification reaction of its participation is an irreversible reaction, the grease conversion rate is fast, transformation efficiency is high.Acetates also can be used as acyl acceptor be used for biofuel preparation (Du, W., Xu, Y.Y., Liu, D.H., Zeng, J.2004, J.Mol.Catal.B:Enzym.30 (3-4), 125-129; Modi, M.K., Reddy, J.R.C., Rao, B.V.S.K., Prasad, R.B.N.2007, Bioresour.Technol.98,1260-1264).Use carbonates and acetates to avoid the toxic action of short chain alcohol to lipase as acyl acceptor.In addition, carbonates and acetates also are that good grease extracts solvent.Comprehensive above-mentioned background introduction, with carbonates or acetates not only as greasy extraction solvent but also as the acyl acceptor of grease transesterificationization or esterification, by preparing biodiesel by biocatalysis one-pot method will be a kind of very promising processing method.
Summary of the invention
The objective of the invention is to have overcome above-mentioned shortcoming of the prior art, a kind of processing method of preparing biodiesel by biocatalysis one-pot method is provided, this processing method design is ingenious, simple to operation, can prepare biofuel fast, thereby enhance productivity greatly, reduce production costs, be suitable for large-scale industrialization and use.
To achieve these goals; the processing method of a kind of preparing biodiesel by biocatalysis one-pot method of the present invention; be characterized in; after will containing the glyceride stock fragmentation; add not only as grease and extract solvent but also as the suitable solvent of grease transesterificationization or esterification acyl acceptor; and biological catalyst, obtain described biofuel thereby react.
Preferably, described suitable solvent is short chain carbonic ether and/or short chain acetic ester.
More preferably, described short chain carbonic ether is selected from one or more of methylcarbonate, diethyl carbonate, dipropyl carbonate and dibutyl carbonate; Described short chain acetic ester is selected from one or more of ritalin, vinyl acetic monomer, n-propyl acetate, Iso Butyl Acetate, n-butyl acetate, isobutyl acetate, n-Amyl acetate and isoamyl acetate.
Preferably, the adding proportion of described suitable solvent is: every gram adds 3～30 milliliters of described suitable solvents described containing in the glyceride stock.
Preferably, described biological catalyst is to derive from the lipase of animal, plant or microorganism or the material of fatty enzyme.
Preferably, the add-on of described biological catalyst is described 3～30% of the theoretical oleaginousness weight of glyceride stock that contains.
Preferably, the temperature of described reaction is 15～65 ℃, and the time of reaction is 4～24 hours.
Preferably, the described glyceride stock that contains comes from one or more of plant materials, animal body, microbe, algae and other grease-contained trade waste or residue.
Therefore, concrete production process of the present invention is:
(1) contains glyceride stock and carry out suitable fragmentation;
(2) ratio that contains 3～30 milliliters of glyceride stocks in every gram adds selected suitable solvent;
(3) add based on the biological catalyst that contains the theoretical oleaginousness weight 3～30% of glyceride stock,, can produce biofuel in 15～65 ℃ of reactions 4～24 hours.
Beneficial effect of the present invention is specific as follows:
1. the use of novel acyl acceptor.With carbonates during as acyl acceptor, the intermediate product carbonic acid monoacyl ester of carbonic ether and glyceryl ester transesterification reaction is in case generate and resolve into pure and mild carbonic acid gas rapidly, and carbonic acid gas is emitted; thereby the continuous forward of reaction is carried out; therefore the grease conversion rate is fast, and the transformation efficiency height reaches nearly 100%., do not have glycerine to generate in the product, thereby avoided its side effect as acyl acceptor with acetates; In addition, carbonates or acetates replace traditional short chain alcohol as acyl acceptor, have avoided in the traditional method short chain alcohol to the toxic action of biological catalyst, thereby have prolonged the life-span of biological catalyst and increased the reusability of biological catalyst;
2. carbonates or acetates are extracted the application of solvent as new oil.Carbonates and acetates are the green solvents that integrates spatter property and security, and they all have good solubility property to grease, can substitute traditional inflammable, explosive solvent and be used for greasy extraction;
3. the exploitation of " one kettle way " preparation biodiesel technology.With carbonates or acetates not only as greasy extraction solvent but also as the acyl acceptor of grease transesterificationization or esterification, utilize biological catalyst catalysis " one kettle way " preparation biofuel, greasy extraction and greasy esterification or transesterification metallization processes are united two into one, design ingenious, simple to operation, avoided greasy extraction refining, processing step is simplified greatly, can prepare biofuel fast, thereby enhance productivity greatly, reduce production costs, be suitable for large-scale industrialization and use;
The present invention no matter from production technique still from production cost, all have fabulous industrial applications prospect.
Embodiment
In order more to be expressly understood technology contents of the present invention, describe in detail especially exemplified by following examples.
Embodiment 1
After the suitable pulverizing of Semen Brassicae campestris, get 10 grams and mix in tool plug triangular flask with 30 milliliters of methylcarbonates, place temperature control shaking table adjusted temperature to 15 ℃, the biological catalyst that adds theoretical oleaginousness 3% begins reaction, reacted 20-24 hour, bio-diesel yield reaches more than 85%.The biological catalyst of Shi Yonging is the lipase that derives from the plant castor seeds herein, smash homogenate to pieces after castor seeds in the dark germinateed three days, again in 10,000 rev/min centrifugal 30 minutes, collect the upper strata liposome, dry back in vacuum freeze drier with the liposome after the extracted with diethyl ether lipid of 5 times of volumes as biological catalyst.
Embodiment 2
After the suitable pulverizing of Semen Brassicae campestris, get 10 grams and mix in tool plug triangular flask with 60 milliliters of diethyl carbonates, place temperature control shaking table adjusted temperature to 25 ℃, the biological catalyst that adds theoretical oleaginousness 6% begins reaction, reacted 15-20 hour, bio-diesel yield reaches more than 90%.The biological catalyst of Shi Yonging is with embodiment 1 herein.
Embodiment 3
After the suitable pulverizing of Semen Brassicae campestris, get 10 grams and mix in tool plug triangular flask with 120 milliliters of ritalins, place temperature control shaking table adjusted temperature to 35 ℃, the biological catalyst that adds theoretical oleaginousness 12% begins reaction, reacted 10-15 hour, bio-diesel yield reaches more than 92%.The biological catalyst of Shi Yonging is with embodiment 1 herein.
Embodiment 4
After the suitable pulverizing of Semen Brassicae campestris, get 10 grams and mix in tool plug triangular flask with 240 milliliters of vinyl acetic monomers, place temperature control shaking table adjusted temperature to 50 ℃, the biological catalyst that adds theoretical oleaginousness 24% begins reaction, reacted 4-10 hour, bio-diesel yield reaches more than 95%.The biological catalyst of Shi Yonging is with embodiment 1 herein.
Embodiment 5
With the fatty tankage of butchery after suitably pulverizing, getting 10 grams mixes in tool plug triangular flask with 50 milliliters of dipropyl carbonates, place temperature control shaking table adjusted temperature to 20 ℃, the biological catalyst that adds theoretical oleaginousness 5% begins reaction, reacted 20-24 hour, bio-diesel yield reaches more than 83%.The biological catalyst of Shi Yonging is the lipase that derives from animal pancreas herein, with the Pancreas Sus domestica is raw material, extract removal lipid, protein precipitation by organic solution, utilize again and saltout and gel chromatography separation and purification albumen, adopt vacuum lyophilization to obtain porcine pancreatic lipase enzyme powder, the enzyme powder is dissolved in the phosphoric acid buffer of pH7.0, added absorption with macroporous adsorbent resin again 6 hours, filter immobilized lipase as biological catalyst.
Embodiment 6
With the fatty tankage of butchery after suitably pulverizing, getting 10 grams mixes in tool plug triangular flask with 100 milliliters of dibutyl carbonates, place temperature control shaking table adjusted temperature to 30 ℃, the biological catalyst that adds theoretical oleaginousness 10% begins reaction, reacted 15-20 hour, bio-diesel yield reaches more than 87%.The biological catalyst of Shi Yonging is with embodiment 5 herein.
Embodiment 7
With the fatty tankage of butchery after suitably pulverizing, getting 10 grams mixes in tool plug triangular flask with 200 milliliters of n-propyl acetates, place temperature control shaking table adjusted temperature to 40 ℃, the biological catalyst that adds theoretical oleaginousness 20% begins reaction, reacted 10-15 hour, bio-diesel yield reaches more than 90%.The biological catalyst of Shi Yonging is with embodiment 5 herein.
Embodiment 8
With the fatty tankage of butchery after suitably pulverizing, getting 10 grams mixes in tool plug triangular flask with 260 milliliters of n-butyl acetates, place temperature control shaking table adjusted temperature to 55 ℃, the biological catalyst that adds theoretical oleaginousness 26% begins reaction, reacted 4-10 hour, bio-diesel yield reaches more than 92%.The biological catalyst of Shi Yonging is with embodiment 5 herein.
Embodiment 9
After the suitable fragmentation of oil-containing microorganism cells, getting 10 grams mixes in tool plug triangular flask with 70 milliliters of methylcarbonates, place temperature control shaking table adjusted temperature to 25 ℃, the biological catalyst that adds theoretical oleaginousness 7% begins reaction, reacted 20-24 hour, bio-diesel yield reaches more than 88%.The biological catalyst of Shi Yonging is separated fat Ye Shi zymic lipase for deriving from herein, separate fat Ye Shi yeast through 3.7 liters of ferment tanks after 4 days, the centrifugal collection supernatant of fermented liquid, get the lipase meal with acetone precipitation, meal is dissolved in the phosphoric acid buffer of pH7.0, add absorption with macroporous adsorbent resin again after 6 hours, filter immobilized lipase as biological catalyst.
Embodiment 10
After the suitable fragmentation of oil-containing microorganism cells, getting 10 grams mixes in tool plug triangular flask with 140 milliliters of diethyl carbonates, place temperature control shaking table adjusted temperature to 35 ℃, the biological catalyst that adds theoretical oleaginousness 14% begins reaction, reacted 15-20 hour, bio-diesel yield reaches more than 90%.The biological catalyst of Shi Yonging is with embodiment 9 herein.
Embodiment 11
After the suitable fragmentation of oil-containing microorganism cells, getting 10 grams mixes in tool plug triangular flask with 220 milliliters of Iso Butyl Acetates, place temperature control shaking table adjusted temperature to 45 ℃, the biological catalyst that adds theoretical oleaginousness 22% begins reaction, reacted 10-15 hour, bio-diesel yield reaches more than 94%.The biological catalyst of Shi Yonging is with embodiment 9 herein.
Embodiment 12
After the suitable fragmentation of oil-containing microorganism cells, getting 10 grams mixes in tool plug triangular flask with 280 milliliters of isobutyl acetates, place temperature control shaking table adjusted temperature to 60 ℃, the biological catalyst that adds theoretical oleaginousness 28% begins reaction, reacted 4-10 hour, bio-diesel yield reaches more than 98%.The biological catalyst of Shi Yonging is with embodiment 9 herein.
Embodiment 13
After the suitable fragmentation of oil-containing alga cells, get 10 grams and mix in tool plug triangular flask with 90 milliliters of dipropyl carbonates, place temperature control shaking table adjusted temperature to 30 ℃, the biological catalyst that adds theoretical oleaginousness 9% begins reaction, reacted 20-24 hour, bio-diesel yield reaches more than 84%.The biological catalyst of Shi Yonging is for being showed in Pseudomonas fluorescens lipase LIP68 gene the surface display lipase whole-cell biological catalyzer that the pichia yeast surface obtains herein.Lipase LIP68 gene is connected on the pichia yeast surface display carrier, imports the engineering bacteria that pichia yeast makes up surface display LIP68 gene, engineering bacteria after fermenting after the centrifugal collection thalline vacuum lyophilization directly as biological catalyst.
Embodiment 14
After the suitable fragmentation of oil-containing alga cells, getting 10 grams mixes in tool plug triangular flask with 160 milliliters of dibutyl carbonates, place temperature control shaking table adjusted temperature to 40 ℃, the biological catalyst that adds theoretical oleaginousness 16% begins reaction, reacted 15-20 hour, bio-diesel yield reaches more than 91%.The biological catalyst of Shi Yonging is with embodiment 13 herein.
Embodiment 15
After the suitable fragmentation of oil-containing alga cells, getting 10 grams mixes in tool plug triangular flask with 240 milliliters of n-Amyl acetates, place temperature control shaking table adjusted temperature to 50 ℃, the biological catalyst that adds theoretical oleaginousness 24% begins reaction, reacted 10-15 hour, bio-diesel yield reaches more than 95%.The biological catalyst of Shi Yonging is with embodiment 13 herein.
Embodiment 16
After the suitable fragmentation of oil-containing alga cells, getting 10 grams mixes in tool plug triangular flask with 300 milliliters of isoamyl acetates, place temperature control shaking table adjusted temperature to 65 ℃, the biological catalyst that adds theoretical oleaginousness 30% begins reaction, reacted 4-10 hour, bio-diesel yield reaches more than 97%.The biological catalyst of Shi Yonging is with embodiment 13 herein.
To sum up, the design of the processing method of preparing biodiesel by biocatalysis one-pot method of the present invention is ingenious, simple to operation, can prepare biofuel fast, thereby enhance productivity greatly, reduces production costs, and is suitable for large-scale industrialization and uses.
In this specification sheets, the present invention is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (8)
1. the processing method of a preparing biodiesel by biocatalysis one-pot method; it is characterized in that; after will containing the glyceride stock fragmentation; add not only as grease and extract solvent but also as the suitable solvent of grease transesterificationization or esterification acyl acceptor; and biological catalyst, obtain described biofuel thereby react.
2. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1 is characterized in that, described suitable solvent is short chain carbonic ether and/or short chain acetic ester.
3. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 2 is characterized in that, described short chain carbonic ether is selected from one or more of methylcarbonate, diethyl carbonate, dipropyl carbonate and dibutyl carbonate; Described short chain acetic ester is selected from one or more of ritalin, vinyl acetic monomer, n-propyl acetate, Iso Butyl Acetate, n-butyl acetate, isobutyl acetate, n-Amyl acetate and isoamyl acetate.
4. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1 is characterized in that, the adding proportion of described suitable solvent is: every gram adds 3～30 milliliters of described suitable solvents described containing in the glyceride stock.
5. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1 is characterized in that, described biological catalyst is to derive from the lipase of animal, plant or microorganism or the material of fatty enzyme.
6. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1 is characterized in that, the add-on of described biological catalyst is described 3～30% of the theoretical oleaginousness weight of glyceride stock that contains.
7. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1 is characterized in that, the temperature of described reaction is 15～65 ℃, and the time of reaction is 4～24 hours.
8. the processing method of preparing biodiesel by biocatalysis one-pot method according to claim 1, it is characterized in that the described glyceride stock that contains comes from one or more of plant materials, animal body, microbe, algae and other grease-contained trade waste or residue.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102002430A (en) * | 2010-10-29 | 2011-04-06 | 广西大学 | Method for leaching vegetable fat with dimethly carbonate |
| CN103074389A (en) * | 2013-02-04 | 2013-05-01 | 东莞市合能微生物能源有限公司 | Method for preparing biodiesel by using biological enzyme |
| US20130192126A1 (en) * | 2012-02-01 | 2013-08-01 | Industry-Academic Cooperation Foundation, Yonsei University | Method for producing biodiesel from algal lipid with dimethyl carbonate |
| CN103275814A (en) * | 2013-06-07 | 2013-09-04 | 华东理工大学 | Method for preparing biodiesel without by-product of glycerin by utilization of high acid value waste oil |
| CN103436368A (en) * | 2013-09-04 | 2013-12-11 | 西安石油大学 | Method for synchronously preparing biodiesel and glycerol carbonate by using alkali-earth oxide as catalyst |
| CN103525798A (en) * | 2012-07-03 | 2014-01-22 | 深圳市绿微康生物工程有限公司 | Biological enzyme catalyst for preparing dimethyl carbonate and preparation method thereof |
| CN106811308A (en) * | 2016-12-23 | 2017-06-09 | 惠州市鑫隆环保科技有限公司 | A kind of preparation method of industrial fuel oil |
| CN108676587A (en) * | 2018-06-08 | 2018-10-19 | 宁夏莱德环保能源有限公司 | It is a kind of to utilize the micro-emulsification fuel oil and its processing method of discarding animal fat preparation |
| CN111440665A (en) * | 2020-04-07 | 2020-07-24 | 西南林业大学 | Method for weak-base esterification of fatty acid and one-step ester exchange of high-acid-value oil |
| CN111471528A (en) * | 2020-04-01 | 2020-07-31 | 福建农林大学 | In-situ conversion method for simultaneously preparing biodiesel and animal feed by taking animal wastes as raw materials |
-
2009
- 2009-07-29 CN CN200910055586A patent/CN101619329A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102002430A (en) * | 2010-10-29 | 2011-04-06 | 广西大学 | Method for leaching vegetable fat with dimethly carbonate |
| CN102002430B (en) * | 2010-10-29 | 2013-06-19 | 广西大学 | Method for leaching vegetable fat with dimethly carbonate |
| US20130192126A1 (en) * | 2012-02-01 | 2013-08-01 | Industry-Academic Cooperation Foundation, Yonsei University | Method for producing biodiesel from algal lipid with dimethyl carbonate |
| CN103525798A (en) * | 2012-07-03 | 2014-01-22 | 深圳市绿微康生物工程有限公司 | Biological enzyme catalyst for preparing dimethyl carbonate and preparation method thereof |
| CN103525798B (en) * | 2012-07-03 | 2015-11-25 | 深圳市绿微康生物工程有限公司 | For the preparation of the biological enzyme agent and preparation method thereof of methylcarbonate |
| CN103074389A (en) * | 2013-02-04 | 2013-05-01 | 东莞市合能微生物能源有限公司 | Method for preparing biodiesel by using biological enzyme |
| CN103275814A (en) * | 2013-06-07 | 2013-09-04 | 华东理工大学 | Method for preparing biodiesel without by-product of glycerin by utilization of high acid value waste oil |
| CN103275814B (en) * | 2013-06-07 | 2015-02-18 | 华东理工大学 | Method for preparing biodiesel without by-product of glycerin by utilization of high acid value waste oil |
| CN103436368A (en) * | 2013-09-04 | 2013-12-11 | 西安石油大学 | Method for synchronously preparing biodiesel and glycerol carbonate by using alkali-earth oxide as catalyst |
| CN106811308A (en) * | 2016-12-23 | 2017-06-09 | 惠州市鑫隆环保科技有限公司 | A kind of preparation method of industrial fuel oil |
| CN108676587A (en) * | 2018-06-08 | 2018-10-19 | 宁夏莱德环保能源有限公司 | It is a kind of to utilize the micro-emulsification fuel oil and its processing method of discarding animal fat preparation |
| CN108676587B (en) * | 2018-06-08 | 2020-10-30 | 宁夏莱德环保能源有限公司 | Micro-emulsified fuel oil prepared from waste animal fat and processing method thereof |
| CN111471528A (en) * | 2020-04-01 | 2020-07-31 | 福建农林大学 | In-situ conversion method for simultaneously preparing biodiesel and animal feed by taking animal wastes as raw materials |
| CN111440665A (en) * | 2020-04-07 | 2020-07-24 | 西南林业大学 | Method for weak-base esterification of fatty acid and one-step ester exchange of high-acid-value oil |
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Application publication date: 20100106 |