CN101612234A - A kind of method of Chinese medicine processing - Google Patents

A kind of method of Chinese medicine processing Download PDF

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CN101612234A
CN101612234A CN200910115695A CN200910115695A CN101612234A CN 101612234 A CN101612234 A CN 101612234A CN 200910115695 A CN200910115695 A CN 200910115695A CN 200910115695 A CN200910115695 A CN 200910115695A CN 101612234 A CN101612234 A CN 101612234A
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chinese medicine
raw material
fructus aurantii
hesperetin
pyranglucoside
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CN101612234B (en
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龚千锋
袁艳金
蒋以号
袁艳
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JIANGXI ZHANGSHU TIANQITANG TRADITIONAL CHINESE MEDICINE DECOCTION PIECES CO Ltd
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JIANGXI ZHANGSHU TIANQITANG TRADITIONAL CHINESE MEDICINE DECOCTION PIECES CO Ltd
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Abstract

The present invention relates to a kind of method of Chinese medicine processing, particularly to the Chinese medicine processing method of Fructus Aurantii.A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 6-24 hour, raw material of Chinese medicine after soaking into the extruding of the casting die of irony again with yoke on the raw material of Chinese medicine, was placed 6-8 days in 30-35 ℃, relative humidity are the environment of 60-80%, take out section earlier, dry again and obtain the prepared slices of Chinese crude drugs; Raw material of Chinese medicine wherein is a Fructus Aurantii.Use the Fructus Aurantii after Chinese medicine processing method of the present invention is handled, can find that through detecting hesperetin is arranged-existence of 7-O-β-D-pyranglucoside.The material of hesperetin class has certain efficacy of drugs usually, and hesperetin-7-O-β-D-pyranglucoside has stronger pharmacologically active.With Fructus Aurantii is that the Chinese medicine of representative is placed under the hot and humid environment and handles, and to adopt the casting die typing of irony be typical Camphor tree group method concocting method.

Description

A kind of method of Chinese medicine processing
Technical field
The present invention relates to a kind of method of Chinese medicine processing, mainly is the Chinese medicine processing method at Fructus Aurantii.
Background technology
Different processing procedures are influential to the variation of Chinese medicine ingredients, and then influence pharmaceutical properties and quality.For example just pointed out that different processing procedures are influential to the Fructus Aurantii quality in the article of " different processing procedures are to the research of Fructus Aurantii quality influence " of the 23rd volume 4 phases record in " practical Chinese medicine magazine " April in 2007, with the content of water-soluble extractives in cold-maceration mensuration Fructus Aurantii decoction pieces and the different processed product, with content of total flavone in determined by ultraviolet spectrophotometry Fructus Aurantii decoction pieces and the different processed product.The result; The content of stir-baked Fructus Aurantii in bran, bran baking Fructus Aurantii water-soluble extractives is all apparently higher than the Fructus Aurantii decoction pieces, but total yellow content of succeeding all slightly descends.Relatively stir-baked Fructus Aurantii in bran and bran baking Fructus Aurantii is found, two kinds of processing procedures are to the active constituent content influence of Fructus Aurantii.
Traditional Fructus Aurantii processing technique is: gets the Fructus Aurantii crude drug, removes impurity, soaked about 1 hour, clean and pull out with clear water, and moistening 24-28 hour, shave when extremely interior free surface moisture is consistent, drying, the flesh that sieve goes to come off is examined.Traditional Fructus Aurantii processing technique is not concocted under hot and humid condition, and internal component hesperetin-7-O-β-D-pyranglucoside do not occur through detecting after changing not obvious, traditional Fructus Aurantii process of preparing Chinese medicine end.
The material of hesperetin, glycoside has certain drug action; for example " Separation of Natural Products " rolled up for 6 phases in 2006 4; " 1-3; the drug efficacy study of 8 hesperetins and application " put down in writing drug efficacy study and the application of Sichuan University's life sciences institute living resources and key lab of the ecological environment Ministry of Education to hesperetin: hesperetin (Hesperetin) is ubiquitous a kind of flavonoid material in the orange, has antioxidation, anti-inflammatory, the reaction of resistance state property, blood fat reducing, protection cardiovascular and antineoplastic drug action.
" Nanjing Normal University's journal: natural science edition " 2008 31 3 phases of volume, " hesperetin is to the influence of vascular endothelial cell NO secretory function " put down in writing the influence of hesperetin to Human umbilical vein endothelial cells (HUVECs) NO secretory function, inquires into its mechanism of action.Adopt the DAN method to measure the level of HUVECs secretion NO for this reason, estimate the estrogenic activity of hesperetin with short proliferation experiment of human breast carcinoma MCF-7 cell (estrogen receptor positive) and reporter gene model experiment.The result is presented under the lower condition of endogenous estrogen level, and hesperetin can promote HUVECs secretion NO, and be dose dependent in 12.5-100 μ moVL concentration range.This effect can be by estrogen receptor antagon ICI182, and 780 and the actinomycin D blocking-up.Hesperetin and E2 do the time spent simultaneously, and HUVECs secretion NO level is done the time spent separately than both and significantly descended.And under endogenous estrogen level conditions of higher, hesperetin suppresses HUVECs secretion NO.Hesperetin can promote the MCF-7 cell proliferation, and can be by ICI182,780 blocking-up fully, simultaneously, hesperetin can part antagonism E2 to the short proliferation function of MCF-7 cell.In addition, hesperetin can be induced the reporter gene expression of ERa control.Thereby can think that hesperetin belongs to the estrogen receptor partial agonist, vascular endothelial cell NO secretory function is had regulating action, its mechanism relates to the estrogen receptor signal pathway and genetic transcription is regulated.
Summary of the invention
The object of the present invention is to provide a kind of method of Chinese medicine processing.
Technical scheme of the present invention:
A kind of method of Chinese medicine processing cuts out the capsula interna of raw material of Chinese medicine with cutter, cleans silt, water infiltration 6-24 hour, with the raw material of Chinese medicine after the casting die extruding infiltration of irony, again with yoke on the raw material of Chinese medicine, under hot and humid environment, concoct, take out section earlier, dry again and obtain the prepared slices of Chinese crude drugs; Raw material of Chinese medicine wherein is a Fructus Aurantii.
A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 6-24 hour, raw material of Chinese medicine after soaking into the extruding of the casting die of irony again with yoke on the raw material of Chinese medicine, was placed 6-8 days in 30-35 ℃, relative humidity are the environment of 60-80%, take out section earlier, dry again and obtain the prepared slices of Chinese crude drugs; Raw material of Chinese medicine wherein is a Fructus Aurantii.
A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 6-24 hour, with the raw material of Chinese medicine after the casting die extruding infiltration of irony, again with yoke on the raw material of Chinese medicine, in being the environment of 60-80%, 30-35 ℃, relative humidity placed 6-8 days, take out section earlier, dry the prepared slices of Chinese crude drugs that obtain containing hesperetin-7-O-β-D-pyranglucoside again, raw material of Chinese medicine wherein is a Fructus Aurantii.
The casting die of irony can be the weight that metallic iron is made, such as anchor.Raw material of Chinese medicine after the infiltration is finalized the design than being easier to anchor extruding back.
With Fructus Aurantii is that the Chinese medicine of representative is placed under the hot and humid environment and handles, and to adopt the casting die typing of irony be typical Camphor tree group method concocting method, and Camphor tree group method concocting method is the characteristic Chinese medicine processing method of passing on from generation to generation always, does not announce to the external world.
Owing to adopt the process of preparing Chinese medicine method under the hot and humid condition of the present invention, promoted the variation of Fructus Aurantii internal component greatly, use the Fructus Aurantii after Chinese medicine processing method of the present invention is handled, can find that through detecting hesperetin is arranged-existence of 7-O-β-D-pyranglucoside.And hesperetin-7-O-β-D-pyranglucoside was never reported in the Fructus Aurantii composition that in the past document relates to.This proves that Chinese medicine processing method of the present invention has conclusive effect to the variation of the internal component of Fructus Aurantii really.
The hesperetin of hesperetin-7-O-β-D-pyranglucoside and existing bibliographical information, the material of glycoside have similar chemical constitution, thereby similar efficacy of drugs is also arranged.
Hesperetin-7-O-β-concrete the detection method of D-pyranglucoside: the chemical constitution study of concocting the back Fructus Aurantii, have two new chromatographic peaks to produce (seeing No. 5 peaks and No. 6 peaks among Fig. 2) in the high-efficient liquid phase chromatogram (HPLC) discovery is concocted Fructus Aurantii through method of the present invention after, the present invention carries out extraction separation to the chemical constituent in the processed product of Fructus Aurantii and adopts 1H-NMR, 13Spectrum Analysis technology such as C-NMR, HMQC, HMBC identify that in conjunction with the structure of the chemical compound that physicochemical property obtains separation wherein No. 6 peaks are hesperetin-7-O-β-D-pyranglucoside.
The structure of hesperetin-7-O-β-D-glucopyranoside compound is identified: white powder (ethanol), mp223-225 ℃, AlCl 3Its fluorescence is significantly strengthened. 1H-NMR (DMSO-d6,400MHz) spectrum δ: 5.50 (1H, dd, J=3,9.7Hz), 2.76 (J=14.5Hz), 3.29 (1H, d J=12.9Hz) are 2H on the typical flavanone parent nucleus, the proton signal of 3H for 1H, d.(1H, s), 6.12 (1H is 5,7 two to replace H-6 and two proton signals of H-8 on the flavone A rings s) to δ 6.14, and (3H m) is 3 proton signals on the B ring to δ 6.95-6.87.(1H s) is the 5-OH signal to δ 12.05, and (1H s) is the phenolic hydroxyl group proton signal to δ 9.16, and (3H s) is proton on the methoxyl group to δ 3.76. 13The carbon spectrum data contrast basically identical of the hesperetin 7-O-β-D-pyranglucoside in C-NMR data (seeing Table 1) and the document, by 1H- 1HCOSY, HMQC, HMBC spectrum also confirm consistent with bibliographical information, are hesperetin 7-O-β-D-pyranglucoside so identify this chemical compound.
Table 1
Figure G2009101156957D00031
Figure G2009101156957D00041
Advantage of the present invention: by under hot and humid environment, concocting, can obtain containing the Fructus Aurantii decoction pieces of new hesperetin-7-O-β-D-pyranglucoside material, the material of hesperetin classification has certain pharmaceutical properties usually, and hesperetin 7-O-β-D-pyranglucoside has stronger pharmacologically active.
Description of drawings
Fig. 1 is that Fructus Aurantii gives birth to product HPLC figure
Fig. 2 is that Fructus Aurantii is through the processed product HPLC figure after concocting
Fig. 3 is the molecular structure of hesperetin 7-O-β-D-pyranglucoside
Specific embodiment
Embodiment 1
A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 6 hours is with the raw material of Chinese medicine after the casting die extruding infiltration of irony, again with yoke on the raw material of Chinese medicine, in being 60% environment, 30 ℃, relative humidity placed 8 days, take out section earlier, dry the prepared slices of Chinese crude drugs that obtain containing hesperetin-7-O-β-D-pyranglucoside again, raw material of Chinese medicine wherein is a Fructus Aurantii.
Embodiment 2
A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 24 hours is with the raw material of Chinese medicine after the casting die extruding infiltration of irony, again with yoke on the raw material of Chinese medicine, in being 80% environment, 35 ℃, relative humidity placed 6 days, take out section earlier, dry the prepared slices of Chinese crude drugs that obtain containing hesperetin-7-O-β-D-pyranglucoside again, raw material of Chinese medicine wherein is a Fructus Aurantii.
Embodiment 3
A kind of method of Chinese medicine processing, cut out the capsula interna of raw material of Chinese medicine with cutter, clean silt, water infiltration 12 hours is with the raw material of Chinese medicine after the casting die extruding infiltration of irony, again with yoke on the raw material of Chinese medicine, in being 70% environment, 33 ℃, relative humidity placed 7 days, take out section earlier, dry the prepared slices of Chinese crude drugs that obtain containing hesperetin-7-O-β-D-pyranglucoside again, raw material of Chinese medicine wherein is a Fructus Aurantii.

Claims (2)

1, a kind of method of Chinese medicine processing, it is characterized in that: the capsula interna that cuts out raw material of Chinese medicine with cutter, clean silt, water infiltration 6-24 hour, with the raw material of Chinese medicine after the casting die extruding infiltration of irony,, under hot and humid environment, concoct again with yoke on the raw material of Chinese medicine, cut into slices after drying in taking-up wash clean surface; Raw material of Chinese medicine wherein is a Fructus Aurantii.
2, a kind of method of Chinese medicine processing, it is characterized in that: the capsula interna that cuts out raw material of Chinese medicine with cutter, clean silt, water infiltration 6-24 hour, raw material of Chinese medicine after soaking into the extruding of the casting die of irony again with yoke on the raw material of Chinese medicine, was placed 14-20 days in 30-35 ℃, relative humidity are the environment of 60-80%, cut into slices after drying in taking-up wash clean surface; Raw material of Chinese medicine wherein is a Fructus Aurantii.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101780168A (en) * 2010-03-08 2010-07-21 江西中医学院 Processing method of Fructus Aurantii
CN104289156A (en) * 2014-10-27 2015-01-21 甘肃中医学院 Method for softening traditional Chinese medicinal material before slicing
CN104490960A (en) * 2015-01-20 2015-04-08 黑龙江中医药大学 Radix sileris processing method
CN106511556A (en) * 2016-11-10 2017-03-22 四川涪丰药业有限公司 Processing method of fructus aurantii
CN115518104A (en) * 2021-06-25 2022-12-27 广州中医药大学(广州中医药研究院) Method for processing fructus aurantii

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101780168A (en) * 2010-03-08 2010-07-21 江西中医学院 Processing method of Fructus Aurantii
CN101780168B (en) * 2010-03-08 2012-05-30 江西中医学院 Processing method of Fructus Aurantii
CN104289156A (en) * 2014-10-27 2015-01-21 甘肃中医学院 Method for softening traditional Chinese medicinal material before slicing
CN104490960A (en) * 2015-01-20 2015-04-08 黑龙江中医药大学 Radix sileris processing method
CN104490960B (en) * 2015-01-20 2018-06-22 黑龙江中医药大学 Windproof processing method
CN106511556A (en) * 2016-11-10 2017-03-22 四川涪丰药业有限公司 Processing method of fructus aurantii
CN115518104A (en) * 2021-06-25 2022-12-27 广州中医药大学(广州中医药研究院) Method for processing fructus aurantii

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