CN101608197B - Method for preparing oligomeric isomeric maltose by adopting rice as raw material - Google Patents
Method for preparing oligomeric isomeric maltose by adopting rice as raw material Download PDFInfo
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- CN101608197B CN101608197B CN2009103048478A CN200910304847A CN101608197B CN 101608197 B CN101608197 B CN 101608197B CN 2009103048478 A CN2009103048478 A CN 2009103048478A CN 200910304847 A CN200910304847 A CN 200910304847A CN 101608197 B CN101608197 B CN 101608197B
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Abstract
The invention discloses a method for preparing oligomeric isomeric maltose by adopting rice as raw material, comprising the following steps of: (1) removing the impurities of crude bran, rice hulls and the like in the rice, adding water for soaking, grinding the syrup into grout, and filtering; (2) adding Alpha amylase, Beta amylase and pullulanase, stirring and mixing evenly; (3) carrying out saccharification and liquefaction and discharging slag; (4) adding branching enzyme and bacterial diastatic enzyme, carrying out saccharification, isomerization and filtering; (5) neutralizing and decoloring; (6) refining material liquid with a secondary ion exchange mode; and (7) evaporating and concentrating. The obtained oligomeric isomeric maltose has the IMO content of 90 to 94% (calculated by dried matter).
Description
Technical field
The present invention relates to a kind of preparation method of oligomeric isomeric maltose, especially relating to a kind of is the method that raw material is produced oligomeric isomeric maltose with rice.
Background technology
Oligomeric isomeric maltose (Isomaltooligosacchride, be called for short IMO) also claims bifurcation oligose, be by glucose with the monose number of α (1 → 6) glycosidic link be combined at 2～5 oligose that do not wait, have multiple physiological hygiene function.Because molecular conformation is different with common maltose, for the purpose of difference, and be called oligomeric isomeric maltose.Oligomeric isomeric maltose is widely used in various health nutrient tonics and the food as a kind of important functional foodstuff base material with unique physiological function.Obvious because of its health-care effect, caused the extensive concern of countries in the world, all there is production in Japan, the U.S., France, Holland, Belgium and China Taiwan.
At present, the main raw material of producing the Dian Fentang that comprises oligomeric isomeric maltose in the world is a W-Gum, about 3,000 yuan/ton of prices, and about 10,000 yuan/ton of the oligomeric isomeric maltose sale price of producing, and added value is very high.The U.S. utilizes national corn to be raw material, greatly develops starch refine dsugar, and the annual production increase is very fast, and present various starch sugar procut ultimate productions reach more than 3,000 ten thousand/year ton, considerably beyond sucrose and beet sugar ultimate production.
Rice is the main food of East Asia, South East Asia and South Asia region, and whole world annual production reaches about 400,000,000 tons.China is the largest production and the country of consumption of rice, produces about 1.9 hundred million tons of rice per year.The main component of rice is starch and protein, and wherein starch content accounts for about 80%.With rice particularly low value rice low value rice such as (crack rice) old rice be the oligomeric isomeric maltose of raw material production high added value, production cost should be lower than W-Gum.
Summary of the invention
The object of the present invention is to provide a kind of is the method that raw material is produced oligomeric isomeric maltose with rice.
Purpose of the present invention is achieved by the following technical programs, and it may further comprise the steps:
(1) remove impurity such as chaff powder in the raw material rice (can be crack rice, low value rice such as old rice), rice husk, add and be equivalent to rice weight 3-5 water logging bubble doubly 3-7 hour, defibrination becomes slurries, crosses the 60-80 mesh filter screen;
(2) enzyme-added sizing mixing: adjustment slurry pH value 5.6-6.1, slurries Baume concentration are 16-19Be °, add αDian Fenmei, βDian Fenmei and Pullulanase, and the consumption of every kind of enzyme all is equivalent to the 0.08-0.12% of rice weight, stirring and evenly mixing;
(3) saccharification, liquefy, take off slag: slurries were stirred 5-10 hour at 50-60 ℃, be warmed up to 90 ℃ and keep the 8-10 minute enzyme that goes out, control liquefier DE value (dextrose value, i.e. dextrose equivalent value, vehicle economy value) is 2-4, filtering rice gluten and other impurity;
(4) add enzyme glycolysis and isomerization: add the q enzyme that is equivalent to the heavy 0.1-0.2% of rice raw material and bacterium saccharifying enzyme in filtrate, the pH value is adjusted to 5.5-6.5, and filtrate is warmed to 50-60 ℃, kept 15-25 hour, finish isomerization, again filtrate is warmed up to 90 ℃ and keeps the 8-10 minute enzyme that goes out, filter, get feed liquid;
(5) neutralization decolouring: add the activated carbon decolorizing agent, activated carbon decolorizing agent consumption is equivalent to the 1-5% of rice weight, under the room temperature, filters feed liquid, makes feed liquid as clear as crystal, no carbon granule, foreign matter;
(6) ion-exchange:, make feed liquid as clear as crystal, free from extraneous odour, transparence 〉=95%, pH value 5.1-6.1 with the refining feed liquid of secondary ions exchanged form;
(7) concentrate: with the feed liquid evaporation concentration to concentration 75-85wt%.
High performance liquid chromatography by national relevant industries standard QB/T 2491-2004 regulation detects, and in this oligomeric isomeric maltose slurry or the oligomeric isomeric maltose powder, IMO content reaches 90-94% (dry-matter meter).
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
(1) impurity such as the chaff powder in the removing raw material rice, rice husk, adding the water logging that is equivalent to 4 times of rice weight steeped 5 hours, and defibrination becomes slurries, crosses 70 mesh filter screens;
(2) adjust slurry pH value 5.8, slurries Baume concentration is 17Be °, adds αDian Fenmei, βDian Fenmei and Pullulanase, the consumption of every kind of enzyme all be equivalent to the rice raw material heavy 0.1%, stirring and evenly mixing;
(3) slurries were stirred 7 hours at 55 ℃, be warmed up to 90 ℃ and keep 9 minutes enzymes that go out, control liquefier DE value is 3, filters filtering rice gluten and other impurity with pressure filter;
(4) add and be equivalent to the rice raw material and weigh 0.15% q enzyme and bacterium saccharifying enzyme in filtrate, the pH value adjusts to 6, and filtrate is warmed to 55 ℃, keeps 20 hours, finishes isomerization, again filtrate is warmed up to 90 ℃ and keeps 9 minutes enzymes that go out, filtration, feed liquid;
(5) add the activated carbon decolorizing agent, activated carbon decolorizing agent consumption be equivalent to the rice raw material heavy 3%, under the room temperature, filter feed liquid with pressure filter, as clear as crystal to feed liquid, no carbon granule, foreign matter;
(6) with the refining feed liquid of secondary ions exchanged form, make feed liquid as clear as crystal, free from extraneous odour, transparence 〉=95%, pH value 5.6;
(7) adopt the quadruple effect falling film evaporator to be evaporated to concentration 80wt%.
Detect by the high performance liquid chromatography of stipulating among the national relevant industries standard QB/T 2491-2004, in this oligomeric isomeric maltose slurry or the oligomeric isomeric maltose powder, IMO content reaches 91% (dry-matter meter).
Embodiment 2
(1) impurity such as the chaff powder in the removing raw material rice, rice husk, adding the water logging that is equivalent to 3 times of rice weight steeped 7 hours, and defibrination becomes slurries, crosses 60 mesh filter screens;
(2) adjust slurry pH value 5.6, slurries Baume concentration is 19Be °, adds αDian Fenmei, βDian Fenmei and Pullulanase, the consumption of every kind of enzyme all be equivalent to the rice raw material heavy 0.08%, stirring and evenly mixing;
(3) slurries were stirred 5 hours at 50 ℃, be warmed up to 90 ℃ and keep 8 minutes enzymes that go out, control liquefier DE value is 2, filters filtering rice gluten and other impurity with pressure filter;
(4) add and be equivalent to the rice raw material and weigh 0.1% q enzyme and bacterium saccharifying enzyme in filtrate, the pH value adjusts to 5.5, and filtrate is warmed to 50 ℃, keeps 25 hours, finishes isomerization, is warmed up to 90 ℃ again and keeps 10 minutes enzymes that go out, filter, feed liquid;
(5) add the activated carbon decolorizing agent, activated carbon decolorizing agent consumption be equivalent to the rice raw material heavy 1%, under the room temperature, filter with pressure filter, make feed liquid as clear as crystal, no carbon granule, foreign matter;
(6) with the refining feed liquid of secondary ions exchanged form, make feed liquid as clear as crystal, free from extraneous odour, transparence 〉=95%, the pH value is 5.1:
(7) adopt quadruple effect falling film evaporator evaporation concentration to concentration 84wt%.
High performance liquid chromatography by national relevant industries standard QB/T 2491-2004 regulation detects, and in this oligomeric isomeric maltose slurry, IMO content reaches 92% (dry-matter meter).
Embodiment 3
(1) impurity such as the chaff powder in the removing raw material rice, rice husk, adding the water logging that is equivalent to 5 times of rice weight steeped 3 hours, and defibrination becomes slurries, crosses 80 mesh filter screens;
(2) adjust slurry pH value 6.1, slurries Baume concentration is 16Be °, adds αDian Fenmei, βDian Fenmei and Pullulanase, the consumption of every kind of enzyme all be equivalent to the rice raw material heavy 0.12%, stirring and evenly mixing;
(3) slurries were stirred 10 hours at 60 ℃, be warmed up to 90 ℃ and keep 10 minutes enzymes that go out, control liquefier DE value is 4, filters filtering rice gluten and other impurity with pressure filter;
(4) add and be equivalent to the rice raw material and weigh 0.2% q enzyme and bacterium saccharifying enzyme in filtrate, the pH value adjusts to 6.5, and filtrate is warmed to 60 ℃, keeps 15 hours, finishes isomerization, again filtrate is warmed up to 90 ℃ and keeps 8 minutes enzymes that go out, filtration, feed liquid;
(5) add the activated carbon decolorizing agent, the discoloring agent consumption be equivalent to the rice raw material heavy 5%, under the room temperature, filter feed liquid with pressure filter, make feed liquid as clear as crystal, no carbon granule, foreign matter;
(6) with the refining feed liquid of secondary ions exchanged form, make feed liquid as clear as crystal, free from extraneous odour, transparence 〉=95%, pH value 6.1;
(7) adopt quadruple effect falling film evaporator evaporation concentration to concentration 85wt%.
Detect by the high performance liquid chromatography of stipulating among the national relevant industries standard QB/T 2491-2004, in this oligomeric isomeric maltose slurry, IMO content reaches 93% (dry-matter meter).
Claims (1)
1. one kind is the method that raw material is produced oligomeric isomeric maltose with rice, it is characterized in that it may further comprise the steps:
(1)Remove chaff powder, rice husk impurity in the raw material rice, add and be equivalent to rice weight 3-5 water logging bubble doubly 3-7 hour, defibrination becomes slurries, crosses the 60-80 mesh filter screen;
(2)It is enzyme-added to size mixing: adjustment slurry pH value 5.6-6.1, slurries Baume concentration are 16-19Be °, add the blue enzyme in αDian Fenmei, βDian Fenmei and Pu Shandong, and the consumption of every kind of enzyme all is equivalent to the 0.08-0.12% of rice weight, stirring and evenly mixing;
(3)Saccharification, liquefy, take off slag: slurries were stirred 5-10 hour at 50-60 ℃, be warmed up to 90 ℃ and keep the 8-10 minute enzyme that goes out, control liquefier dextrose equivalent value is 2-4, filters out impurities;
(4) add enzyme glycolysis and isomerization: add the q enzyme that is equivalent to the heavy 0.1-0.2% of rice raw material and bacterium saccharifying enzyme in filtrate, the pH value is adjusted to 5.5-6.5, and filtrate is warmed to 50-60 ℃, kept 15-25 hour, finish isomerization, again filtrate is warmed up to 90 ℃ and keeps the 8-10 minute enzyme that goes out, filter, get feed liquid;
(5)The neutralization decolouring: add the activated carbon decolorizing agent, activated carbon decolorizing agent consumption is equivalent to the 1-5% of rice weight, under the room temperature, filters feed liquid, makes feed liquid as clear as crystal, no carbon granule, foreign matter;
(6)Ion-exchange:, make feed liquid as clear as crystal, free from extraneous odour, transparence 〉=95%, pH value 5.1-6.1 with the refining feed liquid of secondary ions exchanged form;
(7)Concentrate: with the feed liquid evaporation concentration to concentration 75-85wt%.
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| CN2009103048478A CN101608197B (en) | 2009-07-27 | 2009-07-27 | Method for preparing oligomeric isomeric maltose by adopting rice as raw material |
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| CN108300745B (en) * | 2017-12-29 | 2021-04-27 | 齐鲁工业大学 | Method for preparing special modified starch by using complex enzyme |
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Assignee: CHANGSHA XINQI BIOTECHNOLOGY CO., LTD. Assignor: Central South University of Forestry and Technology Contract record no.: 2012430000032 Denomination of invention: Method for preparing oligomeric isomeric maltose by adopting rice as raw material Granted publication date: 20100825 License type: Exclusive License Open date: 20091223 Record date: 20120416 |
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