CN101606511B - Training method for odor discrimination based on flapping reflex of bees to electric shock - Google Patents
Training method for odor discrimination based on flapping reflex of bees to electric shock Download PDFInfo
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- CN101606511B CN101606511B CN2009100947477A CN200910094747A CN101606511B CN 101606511 B CN101606511 B CN 101606511B CN 2009100947477 A CN2009100947477 A CN 2009100947477A CN 200910094747 A CN200910094747 A CN 200910094747A CN 101606511 B CN101606511 B CN 101606511B
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Abstract
The invention provides a training method for odor discrimination based on the flapping reflex of bees to an electric shock. The method comprises the following steps: 1) capturing bees, placing the bees in ice for anesthetization for1 to 2 minutes, and then transferring the bees to tailor-made electric cages by 20 to 40 bees per cage; accommodating for 1 to 3 hours, placing the tailor-made electric cages loaded with the bees in a gas-passage chamber and starting training, wherein, the gas supply lasts 10 seconds, and the electric shock is given 5 seconds after the gas supply and lasts 10 seconds for each training, and conducting the training for the next time after an interval of 20 to 30 minutes, and training each cage of bees for 3 to 5 times; and 3) recording the flapping reflex of the bees upon the gas supply which lasts 10 seconds without electric shock in the detection. The training method of the invention can effectively train the bees to establish the flapping reflex, particularly, the trained bees have the flapping reflex when smelling characteristic odor, in this way, the invention can provide assistance in various operations involving national defense and civil security, such as characteristic odor detection, drug and explosive enforcement, exit-entry security inspection and the like.
Description
Technical field
The present invention relates to the Detection Techniques field of characteristic odor, specifically training method for odor discrimination based on flapping reflex of bees to electric shock.
Background technology
Honeybee has good oxyosmia degree and outstanding ability of learning and memory.By training, they can stimulate the associating memory with specific scent and rewards and punishments, promptly set up conditioning, and produce behavior reaction.Honeybee olfactory conditioning method mainly adopts the bonus requirements normal form of reacting based on the honeybee promuscis in the world at present, honeybee must be fixed in this method, and circadian feeding is to guarantee being in certain starvation before its training, and this has brought operational inconvenience for potential application in the future.
Summary of the invention
The purpose of this invention is to provide a kind of smell learning training method of fluttering its wings up and down and reacting based on the honeybee electric shock.
Of the present inventionly in experiment, observe: in special electric cage, honeybee is subjected to the warning behavior that electric shock meeting colony produces " fluttering its wings up and down ", and further find behavior reaction can by training apiology custom flavor and electro photoluminescence unite set up conditioning after, the smell that is presented separately stimulates and brings out.Invented the smell learning training method of fluttering its wings up and down and reacting thus based on the honeybee electric shock.
Concrete grammar of the present invention is as follows:
1. catch at the honeycomb mouth and get honeybee, put into treated honeybee anesthesia in ice 1-2 minute after, honeybee is transferred in the special electric cage 20 to 40 in every cage.
2. after adapting to 1-3 hour, single cage honeybee is put into the conditioning case begin training.During each training, give 10 seconds gas duration, electric shock begins to apply 5 seconds the time after giving gas, continues 10 seconds.Electric shock strength range 6V-9V, about 800mA.Trained every cage honeybee training 3-5 time at interval in 20 to 30 minutes next time.
3. when detecting, give gas 10 seconds, do not have electric shock, the reaction of fluttering its wings up and down of record honeybee.
Training method of the present invention can be trained the reaction of fluttering its wings up and down of honeybee effectively, and trained honeybee just can produce the reaction of fluttering its wings up and down when smelling characteristic odor.This for the detection feature smell, seize quick-fried, multinomial national defence such as entry and exit safety check etc. and civil security work provides help.
Description of drawings
Fig. 1 is principle of the present invention and device schematic diagram;
Fig. 2 is a special electric basket structure schematic diagram of the present invention (side)
Fig. 3 is the vertical view of Fig. 2.
Among Fig. 1, the a-exhaust duct, the b-extraction fan, c-conditioning case, the special electric cage of d-, the e-microphone, the f-computer, g-gives device of air, h-control box (power-on switch and fan swicth).
Among Fig. 2,3,1-feeds and manages 2-electricity cage wooden frame, 3-electrical network.
Embodiment
1. catch at the honeycomb mouth and get honeybee, put into treated honeybee anesthesia in ice 1-2 minute after, honeybee is transferred to (shown in Fig. 2,3) in the special electric cage, 20 to 40 honeybees of every cage mounted.
2. after adapting to 1-3 hour, the special electric cage d that honeybee is housed is put into conditioning case c begin training.During each training, give 10 seconds gas duration, electric shock begins to apply 5 seconds the time after giving gas, continues 10 seconds, electric shock strength range 6V-9V, and about 800mA trained at interval in 20 to 30 minutes next time, and every cage honeybee is trained 3-5 time.
3. when detecting, give gas 10 seconds, do not have electric shock, the reaction of fluttering its wings up and down of record honeybee.
Trainer is: the special electric cage d that some the movable honeybees of freedom are housed is placed among the conditioning case c (size: 14 * 15 * 20 centimetres), and electric cage is placed with nearby and is used to gather the flutter its wings up and down microphone e of sound of honeybee, and microphone connects computer f with record audio information.Power-on switch and fan swicth place in the control box h.
The wide 10cm of special electric cage, high 12cm, long 3.5cm, the cage body adopts wood frame, and (specification: 0.30 millimeter * long 75 millimeters of diameters) press the both positive and negative polarity interphase distribution, the two poles of the earth, homopolarity series connection back connect positive negative electricity respectively to electrical network with acupuncture needle, the wooden frame two sides all covers with electrical network, and outermost layer covers with gauze again.There is diameter 1cm circular hole wooden frame end face center, is used to place the syrup pipe, the honeybee of feeding.
Claims (2)
1. training method for odor discrimination based on flapping reflex of bees to electric shock is characterized in that carrying out according to the following steps:
1) catch and get honeybee, put into treated honeybee anesthesia in ice 1-2 minute after, honeybee is transferred in the special electric cage 20 to 40 in every cage;
2) adaptation was put into the air flue case with the special electric cage that honeybee is housed and is begun training, when training at every turn after 1-3 hour, give 10 seconds gas duration, electric shock begins to apply 5 seconds the time after giving gas, continues 10 seconds, trained every cage honeybee training 3-5 time at interval in 20 to 30 minutes next time; Described special electric cage is: the cage body adopts wood frame, electrical network is pressed the both positive and negative polarity interphase distribution with acupuncture needle, and the two poles of the earth, homopolarity series connection back connect positive negative electricity respectively, and the wooden frame two sides all covers with electrical network, outermost layer covers with gauze again, and wooden frame end face center is useful on the circular hole of placing syrup pipe, the honeybee of feeding;
When 3) detecting, give gas 10 seconds, do not have electric shock, the reaction of fluttering its wings up and down of record honeybee.
2. training method for odor discrimination based on flapping reflex of bees to electric shock according to claim 1, the strength range 6V-9V that it is characterized in that shocking by electricity, 800mA.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100947477A CN101606511B (en) | 2009-07-21 | 2009-07-21 | Training method for odor discrimination based on flapping reflex of bees to electric shock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100947477A CN101606511B (en) | 2009-07-21 | 2009-07-21 | Training method for odor discrimination based on flapping reflex of bees to electric shock |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101606511A CN101606511A (en) | 2009-12-23 |
| CN101606511B true CN101606511B (en) | 2011-05-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100947477A Expired - Fee Related CN101606511B (en) | 2009-07-21 | 2009-07-21 | Training method for odor discrimination based on flapping reflex of bees to electric shock |
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| Country | Link |
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| CN (1) | CN101606511B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2473627A (en) * | 2009-09-17 | 2011-03-23 | Inscentinel Ltd | Insect Conditioning System |
| CN102726316A (en) * | 2012-07-21 | 2012-10-17 | 深圳市华测检测技术股份有限公司 | Animal olfactory sensation motivation training system and method |
| CN103250654B (en) * | 2013-05-06 | 2014-12-31 | 中国科学院昆明动物研究所 | Mouse smell training detector, smell interference simulation device and training method |
| CN103503792B (en) * | 2013-10-11 | 2015-06-03 | 中国科学院西双版纳热带植物园 | Method for training scout bees and letting scout bees fly |
| CN105044111B (en) * | 2015-08-06 | 2017-11-17 | 浙江大学 | A kind of apparatus and method that gas detection is carried out using chainless honeybee |
| CN105454091A (en) * | 2016-01-14 | 2016-04-06 | 中国科学院合肥物质科学研究院 | Experimental device for researching behavioral characteristics of bee under electrical stimulation |
| CN105557545B (en) * | 2016-03-02 | 2018-03-06 | 沈阳医学院 | The learning and memory training system of odoring substance |
| CN108770721A (en) * | 2018-07-02 | 2018-11-09 | 沈阳医学院 | Closed smell training box smell training experiment system |
| CN110074015B (en) * | 2019-04-23 | 2024-02-20 | 江西农业大学 | Bee vision and/or smell learning memory research device |
-
2009
- 2009-07-21 CN CN2009100947477A patent/CN101606511B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 陈艳梅等.吗啡对蜜蜂学习记忆的影响.《生物化学与生物物理进展》.2007,第34卷全文. * |
| 马寿成.蜜蜂将成探测高手.《科学24小时》.2006,(第2期),全文. * |
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| CN101606511A (en) | 2009-12-23 |
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Granted publication date: 20110504 Termination date: 20130721 |