CN101602812A - A kind of preparation method of polyporusum bellatus - Google Patents
A kind of preparation method of polyporusum bellatus Download PDFInfo
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- CN101602812A CN101602812A CNA2009100272471A CN200910027247A CN101602812A CN 101602812 A CN101602812 A CN 101602812A CN A2009100272471 A CNA2009100272471 A CN A2009100272471A CN 200910027247 A CN200910027247 A CN 200910027247A CN 101602812 A CN101602812 A CN 101602812A
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Abstract
The present invention relates to a kind of preparation method of easy and simple to handle, the polyporusum bellatus that pollutes little, equipment less investment, processing step adds 4-10 times of water gaging decoction 1-3 time for getting the umbellate pore furgus meal, each 2 hours, filter merging filtrate, concentrate, adding ethanol alcohol is sink to and contains alcohol amount 50-75%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the macroporous adsorptive resins, the water wash-out is collected 2-5 times of column volume elutriant, concentrate, cryodrying is pulverized promptly.Adopt the present invention to prepare polyporusum bellatus, the product purity height is easy to realize the industrialization amplification.
Description
Technical field
The present invention relates to a kind of preparation method of polyporusum bellatus, especially a kind of method of from plant, extracting polyporusum bellatus.
Background technology
Polyporusum bellatus is a kind of effective constituent that contains in the Chinese medicine umbellate pore furgus (polyporus).Umbellate pore furgus is a kind of medicinal fungi, classifies treasure as in " Dragon Lord book on Chinese herbal medicine warp " lining, and umbellate pore furgus can be controlled inflammation, diuresis, toxin expelling, liver protecting, Patients with Fatty Liver is had auxiliary curative effect.Studies show that recently polyporusum bellatus is a kind of good immunomodulator, can grow by anticancer, can improve the spirit and the appetite of tumour patient.Polyporusum bellatus can also significantly improve the hepatitis symptom, liver tissue injury is had repair, efficacy consolidation.And can sun be turned out cloudy.Human body absorbs good to polyporusum bellatus, take for a long time to have no side effect.
In the prior art, the extraction separation of polyporusum bellatus mainly adopts water extraction, and organic solvent is handled, but the content of the polyporusum bellatus that these extraction processes obtain is low, and is seriously polluted, and unfavorable big production operation.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation method who is beneficial to big production operation, polyporusum bellatus that product purity is high.
For solving the problems of the technologies described above, the present invention adopts following technical proposal:
Get the umbellate pore furgus meal, add 4-10 times of water gaging and decoct each 2 hours 1-3 time, filter, merging filtrate concentrates, adding ethanol alcohol is sink to and contains alcohol amount 50-75%, leaves standstill the precipitation water dissolution 24 hours, filter, filtrate concentrates, and joins on the macroporous adsorptive resins, the water wash-out is collected 2-5 times of column volume elutriant, concentrates, cryodrying is pulverized promptly.
The amount of water that decocts is preferably 8 times of amounts.
The number of times that decocts is preferably 2 times.
The alcohol precipitation terminal point is preferably to containing alcohol amount 60%.
The model of macroporous adsorbent resin is a kind of among H-1020, X-5, AB-8, H-103, D-101, DA-201, DS-401, the DM-301.
Elutriant is preferably collects 4 times of column volumes.
Adopt technique scheme to prepare polyporusum bellatus, easy and simple to handle, pollute less, equipment drops into for a short time, is beneficial to big production operation.
Below in conjunction with embodiment the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1
Get umbellate pore furgus meal 10Kg, add 4 times of water gagings and decoct 1 time, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 50%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the H-1020 macroporous adsorptive resins, the water wash-out is collected 2 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 561g, detect through spectrophotometry, purity is 95.6%.
Embodiment 2
Get umbellate pore furgus meal 10Kg, add 10 times of water gagings and decoct 3 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 70%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the X-5 macroporous adsorptive resins, the water wash-out is collected 5 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 998g, detect through spectrophotometry, purity is 92.1%.
Embodiment 3
Get umbellate pore furgus meal 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the AB-8 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 861g, detect through spectrophotometry, purity is 98.4%.
Embodiment 4
Get umbellate pore furgus meal 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the H-103 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 827g, detect through spectrophotometry, purity is 94.8%.
Embodiment 5
Get umbellate pore furgus meal 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the D-101 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 856g, detect through spectrophotometry, purity is 97.9%.
Embodiment 6
Get umbellate pore furgus meal 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the DA-201 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 856g, detect through spectrophotometry, purity is 97.9%.
Embodiment 7
Get umbellate pore furgus meal 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the DS-401 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 818g, detect through spectrophotometry, purity is 94.5%.
Embodiment 8
Get umbellate pore furgus phase powder 10Kg, add 8 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate concentrates, and adding ethanol alcohol is sink to and contains alcohol amount 60%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the DM-301 macroporous adsorptive resins, the water wash-out is collected 4 times of column volume elutriants, concentrate, cryodrying is pulverized, promptly get polyporusum bellatus 789g, detect through spectrophotometry, purity is 93.6%.
Claims (6)
1, a kind of preparation method of polyporusum bellatus is characterized in that described method comprises the following steps: to get the umbellate pore furgus meal, adds 4-10 times of water gaging and decocts 1-3 time, each 2 hours, filter merging filtrate, concentrate, adding ethanol alcohol is sink to and contains alcohol amount 50-75%, leaves standstill 24 hours, the precipitation water dissolution filters, and filtrate concentrates, join on the macroporous adsorptive resins, the water wash-out is collected 2-5 times of column volume elutriant, concentrate, cryodrying is pulverized promptly.
2, according to the preparation method of the described a kind of polyporusum bellatus of claim 1, the amount of water that it is characterized in that described decoction is 8 times of amounts.
3, according to the preparation method of the described a kind of polyporusum bellatus of claim 1, the number of times that it is characterized in that described decoction is 2 times.
4,, it is characterized in that described alcohol is sink to that to contain alcohol amount be 60% according to the preparation method of the described a kind of polyporusum bellatus of claim 1.
5, according to the preparation method of the described a kind of polyporusum bellatus of claim 1, the model that it is characterized in that described macroporous adsorbent resin is a kind of among H-1020, X-5, AB-8, H-103, D-101, DA-201, DS-401, the DM-301.
6,, it is characterized in that 4 times of column volumes of described elutriant collection according to the preparation method of the described a kind of polyporusum bellatus of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100272471A CN101602812A (en) | 2009-05-26 | 2009-05-26 | A kind of preparation method of polyporusum bellatus |
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| CNA2009100272471A CN101602812A (en) | 2009-05-26 | 2009-05-26 | A kind of preparation method of polyporusum bellatus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276753A (en) * | 2011-09-13 | 2011-12-14 | 陕西理工学院 | Method for preparing polyporus selenium polysaccharide |
| CN102451163A (en) * | 2010-10-28 | 2012-05-16 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN104623310A (en) * | 2015-01-09 | 2015-05-20 | 郑州师范学院 | Method for processing fresh dendrobium devonianum stem powder |
| CN107698695A (en) * | 2017-09-12 | 2018-02-16 | 广州中医药大学第二附属医院 | Homogeneous polysaccharide with immunomodulatory action and preparation method thereof |
| CN110954642A (en) * | 2018-09-26 | 2020-04-03 | 上海医药工业研究院 | Detection method of grifola quality and construction method of fingerprint spectrum thereof |
-
2009
- 2009-05-26 CN CNA2009100272471A patent/CN101602812A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451163A (en) * | 2010-10-28 | 2012-05-16 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN102451163B (en) * | 2010-10-28 | 2013-07-31 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN102276753A (en) * | 2011-09-13 | 2011-12-14 | 陕西理工学院 | Method for preparing polyporus selenium polysaccharide |
| CN104623310A (en) * | 2015-01-09 | 2015-05-20 | 郑州师范学院 | Method for processing fresh dendrobium devonianum stem powder |
| CN107698695A (en) * | 2017-09-12 | 2018-02-16 | 广州中医药大学第二附属医院 | Homogeneous polysaccharide with immunomodulatory action and preparation method thereof |
| CN110954642A (en) * | 2018-09-26 | 2020-04-03 | 上海医药工业研究院 | Detection method of grifola quality and construction method of fingerprint spectrum thereof |
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Application publication date: 20091216 |