CN102302561A - Method for preparing belvedere fruit total saponins - Google Patents
Method for preparing belvedere fruit total saponins Download PDFInfo
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- CN102302561A CN102302561A CN201110264180A CN201110264180A CN102302561A CN 102302561 A CN102302561 A CN 102302561A CN 201110264180 A CN201110264180 A CN 201110264180A CN 201110264180 A CN201110264180 A CN 201110264180A CN 102302561 A CN102302561 A CN 102302561A
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- fructus kochiae
- total saponins
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Abstract
The invention belongs to the technical field of biology, and discloses a method for preparing belvedere fruit total saponins. The method comprises the following steps of: performing supercritical CO2 extraction, performing ultrasound extraction, decolorizing by active carbon, drying and the like. The method has the characteristics of high extraction efficiency, short preparation period, high purity of products, low pollution and the like, and industrial production is easy to realize.
Description
Technical field
The invention belongs to biological technical field, particularly relate to a kind of method for preparing of Fructus Kochiae total saponins.
Background technology
The Fructus Kochiae is the chenopod Fructus Kochiae
Kochia scoparia(L.) dry mature fruit of Schrad..Traditional medicine thinks that Fructus Kochiae nature and flavor are hot, bitter, has clearing away heat-damp and promoting diuresis, the dispelling wind for relieving itching effect.Be usually used in treating difficulty and pain in micturition, pudendal pruritus leukorrhagia, rubella, eczema, skin pruritus.Modern study shows, contains a large amount of volatile oil and saponin constituent in the Fructus Kochiae, and volatile oil has the killing pests and suppressing bacteria effect, and saponin has effects such as antiinflammatory, antiallergic, blood sugar lowering, antitumor.
The existing method of from the Fructus Kochiae, extracting total saponins adopts extraction and resin method more.The method that " triterpene of the Chinese medicine Fructus Kochiae and saponin constituent research " is adopted like document is an alcohol reflux, and extracting solution concentrates, defat with petroleum ether, and n-butanol extraction is gone up silicagel column again.Patent (application number: 200510029817) " a kind of effective ingredient in Chinese extract and extraction process thereof of treating anaphylactia "; This patent disclosed method is that the fruit of summer cypress is ground into meal; Extract with 70% alcohol heat reflux earlier; Reclaim ethanol and get thick medicinal extract; Thick medicinal extract can be gone up macroporous resin adsorption after adding water 100ml dissolving; Macroreticular resin in crude drug weight ratio 1: 1-1: 5 ratio dress post; Then with the medicinal extract liquid after the above-mentioned dilution with the flow velocity of 10-50 ml/min by the macroporous absorbent resin post; After passing through fully; Water, 10%-100% ethanol gradient elution; Collect 50%-80% ethanol stream part, vacuum drying.Because it is more to contain oils and fats in the Fructus Kochiae, existing purification process is comparatively loaded down with trivial details, and the cycle is longer, and yield is lower, and content is lower.
Summary of the invention
The objective of the invention is to overcome the deficiency and the technological deficiency of existing method, a kind of method for preparing of Fructus Kochiae total saponins is provided.
The objective of the invention is to realize through following technical scheme: a kind of method for preparing of Fructus Kochiae total saponins is characterized in that following steps:
(1) Fructus Kochiae pulverizing medicinal materials adds extraction kettle, under pressure 15-25MPa, temperature 40-50 ℃, feeds liquid CO
2And entrainer, extracted 1-3 hour;
(2) above-mentioned stripped medical material takes out, and adds 3-10 and doubly measures the 70-99% ethanol ultrasonic extraction 2-3 time, and each 30 minutes, extracting solution added the active carbon reflux decolour 1 hour, destaining solution filtered and recycled reagent, the dry Fructus Kochiae total saponins that gets.
Entrainer in the said step 1) is an acetone soln, and consumption is that the 1-3 of medical material doubly measures.
Said step 2) the supersound extraction power 500-3000W in, frequency 20-60KHz.
Said step 2) active carbon in is a medicinal carbon, and consumption is the 1-3% of destaining solution.
Technical advantage of the present invention is that it is the entrainer supercritical CO that method has adopted earlier with acetone
2Oils and fats, alkaloid and flavone in the extraction Fructus Kochiae extract saponin again, are to utilize saponin to be insoluble to acetone character, have obtained the saponin of higher degree.And people such as Lin Xiuxian to adopt be to be the entrainer supercritical CO with 95% ethanol
2Extraction Fructus Kochiae volatile oil can extract a certain amount of saponin in the extraction process.
Technical advantage of the present invention is that also method adopts supersound extraction, and the cycle is short, and the impurity stripping is few, and energy consumption is lower.
The specific embodiment
To combine the specific embodiment to further specify the present invention below.
Embodiment 1:
Fructus Kochiae pulverizing medicinal materials 40 orders are got 3kg and are added extraction kettle, feed liquid CO with flow velocity 20kg/h
2, 40 ℃ of extracting pressure 15MPa, temperature are set, reach parameter after, feed the 2L/h acetone soln, extract 3 hours, extract is resolved in timing.Extraction is emitted material after finishing, and 70% ethanol ultrasonic (power 800W, frequency 40KHz) that adds 5 times of amounts extracted 30 minutes; Extract 3 times, extracting solution merges and added 200g medicinal carbon reflux decolour 1 hour, after the filtration; Reclaim reagent, vacuum drying gets Fructus Kochiae total saponins 52g, content 87.5%.
Embodiment 2:
Fructus Kochiae pulverizing medicinal materials 60 orders are got 3kg and are added extraction kettle, feed liquid CO with flow velocity 20kg/h
2, 50 ℃ of extracting pressure 25MPa, temperature are set, reach parameter after, feed the 2L/h acetone soln, extract 2 hours, extract is resolved in timing.Extraction is emitted material after finishing; Ultrasonic (the power 500W of 99% ethanol that adds 10 times of amounts; Frequency 60KHz) extracted 30 minutes; Add 3 times of amount ethanol extractions more once; Extracting solution merges and added 300g medicinal carbon reflux decolour 1 hour, after the filtration, reclaims reagent; Vacuum drying gets Fructus Kochiae total saponins 43g, content 92.7%.
Embodiment 3:
Fructus Kochiae pulverizing medicinal materials 60 orders are got 3kg and are added extraction kettle, feed liquid CO with flow velocity 20kg/h
2, 45 ℃ of extracting pressure 23MPa, temperature are set, reach parameter after, feed the 1.5L/h acetone soln, extract 3 hours, extract is resolved in timing.Extraction is emitted material after finishing, and 80% ethanol ultrasonic (power 800W, frequency 60KHz) that adds 6 times of amounts extracted 30 minutes; Extract 2 times, extracting solution merges and added 200g medicinal carbon reflux decolour 1 hour, after the filtration; Reclaim reagent, vacuum drying gets Fructus Kochiae total saponins 48g, content 90.1%.
Claims (4)
1. the method for preparing of a Fructus Kochiae total saponins is characterized in that following steps:
(1) Fructus Kochiae pulverizing medicinal materials adds extraction kettle, under pressure 15-25MPa, temperature 40-50 ℃, feeds liquid CO
2And entrainer, extracted 2-3 hour;
(2) above-mentioned stripped medical material takes out, and adds 3-10 and doubly measures the 70-99% ethanol ultrasonic extraction 1-3 time, and each 30 minutes, extracting solution added the active carbon reflux decolour 1 hour, destaining solution filtered and recycled reagent, the dry Fructus Kochiae total saponins that gets.
2. the method for preparing of Fructus Kochiae total saponins according to claim 1 is characterized in that the entrainer in the said step 1) is an acetone soln, and consumption is that the 1-3 of medical material doubly measures.
3. the method for preparing of Fructus Kochiae total saponins according to claim 1 is characterized in that said step 2) in supersound extraction power 500-3000W, frequency 20-60KHz.
4. the method for preparing of Fructus Kochiae total saponins according to claim 1 is characterized in that said step 2) in active carbon be medicinal carbon, consumption is the 1-3% of destaining solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110264180A CN102302561A (en) | 2011-09-08 | 2011-09-08 | Method for preparing belvedere fruit total saponins |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110264180A CN102302561A (en) | 2011-09-08 | 2011-09-08 | Method for preparing belvedere fruit total saponins |
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| CN102302561A true CN102302561A (en) | 2012-01-04 |
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| CN201110264180A Pending CN102302561A (en) | 2011-09-08 | 2011-09-08 | Method for preparing belvedere fruit total saponins |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109021060A (en) * | 2018-09-26 | 2018-12-18 | 上海诗丹德标准技术服务有限公司 | The preparation method of momordin Ic |
| CN116270774A (en) * | 2023-04-12 | 2023-06-23 | 云南贝泰妮生物科技集团股份有限公司 | Preparation method of fructus kochiae extract, and product and application thereof |
-
2011
- 2011-09-08 CN CN201110264180A patent/CN102302561A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109021060A (en) * | 2018-09-26 | 2018-12-18 | 上海诗丹德标准技术服务有限公司 | The preparation method of momordin Ic |
| CN109021060B (en) * | 2018-09-26 | 2021-05-18 | 上海诗丹德标准技术服务有限公司 | Preparation method of broom cypress fruit saponin Ic |
| CN116270774A (en) * | 2023-04-12 | 2023-06-23 | 云南贝泰妮生物科技集团股份有限公司 | Preparation method of fructus kochiae extract, and product and application thereof |
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Application publication date: 20120104 |