CN101584719B - Application of acanthopanax fruit extractive in preparing medicament for treating hyperlipoidemia - Google Patents
Application of acanthopanax fruit extractive in preparing medicament for treating hyperlipoidemia Download PDFInfo
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Abstract
The invention relates to the application of an acanthopanax fruit extractive in preparing medicament for treating hyperlipoidemia, belonging to the technical field of medicine. An experimentation shows that the acanthopanax fruit extractive has very good function of treating the hyperlipoidemia, therefore, the acanthopanax fruit extractive can be used for preparing medicament for regulating blood fat, health-care products, food additives or compound preparations, and the like, and is mainly used for preparing the medicament for treating the hyperlipoidemia or improving symptoms of the hyperlipoidemia, wherein the medicament or the health-care products include the prior dosage forms, such as a troche, a capsule, a dripping pill, a pilula, a granular formulation, a soft capsule, a syrup, a chewable tablet, a patch, and the like.
Description
Technical field
The present invention relates to the application of Radix Et Caulis Acanthopanacis Senticosi fruit extract in preparation treatment hyperlipidemia medicine.
Background technology
Radix Acanthopanacis Senticosi fruit is the mature fruit of Araliaceae Radix Et Caulis Acanthopanacis Senticosi Acanthopanax senticosus (Rupr.etMaxim) Harms.The dry root and rhizome of Radix Et Caulis Acanthopanacis Senticosi, its stem, leaf are used as medicine, but its fruit research is less, is not used as the report of medicine.Compendium of Materia Medica claims that Radix Et Caulis Acanthopanacis Senticosi is " herbal classic is top grade ", energy " clothes are made light of one's life by commiting suicide anti-old for a long time for invigorating the spleen and replenishing QI, the strong will of hard muscles and bones ".This property of medicine temperature, acrid in the mouth, little hardship, nontoxic, go into the spleen kidney channel.The energy strengthening the body resistance, intelligence promoting and the mind tranquilizing.Cure mainly deficiency of spleen-YANG and kidneyYANG, soreness of the waist and knees, show solicitude for weak, the insomnia, dreaminess, inappetence.Radix Et Caulis Acanthopanacis Senticosi has multiple pharmacologically active, and to contain plurality of active ingredients relevant with this plant for this.At present, people are more to the research of its rhizome and stem and leaf, and the Radix Et Caulis Acanthopanacis Senticosi chemical constituent mainly contains the glycoside composition: experimental results show that Radix Et Caulis Acanthopanacis Senticosi total glucosides is one of its bioactive ingredients.The main component of Radix Acanthopanacis Senticosi root and rhizome is phenolic glycoside class and fat glycosides compound.The content of total glycosides in root and rhizome accounts for 0.6%～0.9% and 0.6%～1.5% (Wang Zhirui of dried medical material weight respectively, Lin Jingming, Zhang Zhongyi. Radix Et Caulis Acanthopanacis Senticosi chemical constituent and pharmacological research progress .2003,26 (8): 603) isolated seven kinds of Radix Et Caulis Acanthopanacis Senticosi glucosides (A, B, C, D, E, F, G), be respectively daucosterol (A-ELeutheroside A, C
35H
57O
6), Syringa oblata Lindl. phenolic glycoside (B-C
17H
24O
9), 7-hydroxyl-6,8-dimethyl coumarin glucoside (B
1-C
17H
20O
10), galactite glycosides (C-C
8H
16O
6), syringaresinol bioside (D and E), eleutheroside A, B, C, D, E, F, the content ratio of G is 8: 30: 10: 12: 4: 2: 1 (Cao Xianlan, Li Zhulian. Radix Et Caulis Acanthopanacis Senticosi experimental study at abroad (summary) [J]. Chinese herbal medicine, 1980,11 (6): 277), the content of Radix Et Caulis Acanthopanacis Senticosi glucoside B glycosides E is higher than root (] Kang JS in the current research stem, etal.Arch Pharm Res.2001,24 (5): 407).Late 1980s is got 5 kinds of new saponin, i.e. eleutheroside As from leaf
1, A
2, A
3, D
3And A
4Back three's aglycon is for first from (Shao CJ, et al.them.pharm Bull.1989,37 (1): 42) that occurring in nature is separated.Wu Lijun etc. get new chemical compound Radix Et Caulis Acanthopanacis Senticosi ketone and new Radix Et Caulis Acanthopanacis Senticosi phenol (necoiwuji-aphenol), and get Resina Ferulae heteroside (fereloyl-sucrose) (Wu Lijun first, Deng. the stem and leaf of Radix Et Caulis Acanthopanacis Senticosi chemical constituent. Acta Pharmaceutica Sinica, 1999,34 (4): 294).Early 1990s Kujawa waits and is separated to two kinds of new saponin (Kujaws ES et al.J.Natprod.1991,54 (4): 1044) from the methanolic extract of Radix Acanthopanacis Senticosi root.
Radix Et Caulis Acanthopanacis Senticosi contain 2.0%～6.0% alkaline polysaccharide and 2.3%～5.7% water soluble polysaccharide (Huang Taikang. conventional Chinese medicine composition and pharmacology handbook. Beijing: Chinese Medicine science and technology publishing house's (front page), 1994 years).China extracts Radix Et Caulis Acanthopanacis Senticosi polysaccharide PES from Radix Et Caulis Acanthopanacis Senticosi, wherein two kinds of glycocalixs are named PES-A and PES-B, molecular weight is respectively 7000 and 7600, the ratio of components of PES-A, glucose: galactose: arabinose=3: 3: 2: 1, be mainly the pyranoid form aldohexose that 1-3a-D-glucopyanosyl and some 1-2 are connected with 1-4.Radix Et Caulis Acanthopanacis Senticosi polysaccharide (ASPS) comprises glucose, fructose, arabinose etc., is one of immune active ingredient.Radix Et Caulis Acanthopanacis Senticosi polysaccharide and ASII and ASIII are homogeneous polysaccharide, belong to the alkali solubility polysaccharide, mean molecule quantity is 150000 and 30000, the glucosan that ASII is made up of single glucose, and ASIII is made up of arabinose, xylose, 4-methoxyl group glucuronic acid, and its monosaccharide mol ratio is 1: 11: 1 a different xylan.Polysaccharide is very similar to root polysaccharide composition in the Radix Acanthopanacis Senticosi fruit, also contains the water soluble polysaccharide with certain hepatoprotective effect.
In addition, Radix Et Caulis Acanthopanacis Senticosi also contains abundant trace element, removes to contain K, Na, Ca, Mg, P, Al outside the trace element such as Si, also contains e, B, Sr, Ba, Zn, Mn, Cu, Ni, Mo, V, Cr, Co, Sn, Bi, Ag, Pb, and trace element such as Ti (Cao Xianlan. Radix Et Caulis Acanthopanacis Senticosi determination of trace elements [J]. Heilungkiang medicine, 1980, (3): 20-21).And nutritional labeling such as aminoacid (defend flat, etc. aminoacid and metallic element analysis in the Acanthopanax brachypus stem, Chinese crude drug, 1998,11 (6): 37).
Hyperlipemia is the name of disease of modern medicine, is the key factor of incidence of coronary heart disease.Motherland's medical science does not have this name of disease, does not have similar record yet.But observe from for many years clinical practice, unbalance with internal organs QI and blood functional disorder and human body wane and wax of yin and yang and produce turbid damp, the stasis of blood is turbid relevant.According to clinical findings, causing coronary disease patient ratio with hyperlipemia has ascendant trend year by year, so the treatment hyperlipemia has very important effect to coronary heart disease.
The research of relevant Radix Acanthopanacis Senticosi fruit at present and use lessly, containing multiple essential trace element such as Fe, Se, Cu, Mn and human body in rarely seen Radix Acanthopanacis Senticosi fruit all must the acid of ammonia tomb.Total saponin content is 0.29% in the fruit.Acute toxicity test.LD
50＞g/kg body weight, show the Radix Acanthopanacis Senticosi fruit extractum nontoxic substantially (Zhang Liping, Chen Liying, etc.The Radix Acanthopanacis Senticosi fruit chemical composition analysis, Northeast China Normal University's journal natural science edition, 1992, (1): 123-127) and water solublity crude polysaccharides (Zhang Liping, Chen Liying, Zhang Yishen.The separation and purification of the research AS-2 of Radix Acanthopanacis Senticosi fruit water soluble polysaccharide and structure are inquired into, journal of biological chemistry, 1993,9 (1): 1-5) and volatile ingredient (Xue Xingya, Yu Wanying, Chen Jiping, Huang Weidong, etc. the gas chromatogram retention parameter is used for the classification of Radix Acanthopanacis Senticosi fruit volatile oil terpenes and terpenes oxide, chromatograph, 2005,23 (4): minority report such as 422-425).The patent of relevant Radix Acanthopanacis Senticosi fruit aspect has lack and red double stabs dry fruit tea and preparation method thereof lack and red double stabs dry fruit tea and preparation method thereof to relate to a kind of novel beverage and processing technology technology thereof; The raw material of this tea constitutes (Wang Wei, application number: 200610010512.1) by the natural wild dahurian rose dry fruit of the wild Radix Et Caulis Acanthopanacis Senticosi dry fruit that accounts for total compatibility weight 30%～70% and 70%～30%.
Do not see the relevant report that utilizes Radix Et Caulis Acanthopanacis Senticosi fruit extract treatment hyperlipidemia.Radix Et Caulis Acanthopanacis Senticosi fruit extract can obtain according to a conventional method, obtains extractum through water extraction, filtration, after concentrating.
Summary of the invention
The invention provides Radix Et Caulis Acanthopanacis Senticosi fruit extract uses in the medicine of preparation treatment hyperlipidemia.
Radix Acanthopanacis Senticosi fruit water extract, Radix Acanthopanacis Senticosi fruit total saponins or Radix Acanthopanacis Senticosi fruit water extract and Radix Acanthopanacis Senticosi fruit total astragalin composition are in preparation treatment hyperlipidemia medicine, health product, the purposes in food additive or the compound preparation.
The Radix Acanthopanacis Senticosi fruit total saponins is meant Radix Acanthopanacis Senticosi fruit behind water extraction, and extracting solution is crossed macroporous adsorbent resin, and water elution is used the 10%-95% ethanol elution instead then to colourless, collects ethanol elution, reclaims the part that ethanol obtains.
Radix Et Caulis Acanthopanacis Senticosi fruit extract of the present invention is when being used for the treatment of the medicinal usage of hyperlipidemia, and it uses effective dose is 20～500mg/ people/sky, and optimal dose is 30～200mg/ people/sky.
When the present invention is used to prepare the medicine for the treatment of hyperlipidemia, its oral or parenteral, all be safe, under oral situation, it can any conventionally form administration, as tablet, capsule, injection, freeze-dried powder, injectable emulsion, powder, drop pill, granule, soft capsule, syrup, chewing agent and patch.
It is to be made of Radix Et Caulis Acanthopanacis Senticosi fruit extract or its pharmaceutical composition excipient with solid or liquid that the present invention prepares the medicine for the treatment of hyperlipidemia, the excipient of solid used herein or liquid is well known in the art, lift several object lessons below, powder is the powder agent that takes orally, its excipient has lactose, starch, paste essence, calcium carbonate, synthetic or puritan filler aluminum, magnesium oxide, magnesium stearate, sodium bicarbonate, dry yeast etc.; The excipient of ointment can use fatty oil, and agnolin, vaseline, glycerol, Cera Flava, wood are cured, white oil, resin, senior water-repelling agent or the hydrophilizing agent that is combined into such as cured.
Beneficial effect of the present invention is, Radix Et Caulis Acanthopanacis Senticosi fruit extract or its pharmaceutical composition are as the medicine of treatment hyperlipidemia, has remarkable advantage evident in efficacy, with low cost, that toxic and side effects is little, the new medicinal usage that Radix Et Caulis Acanthopanacis Senticosi fruit extract is used for the treatment of hyperlipidemia belongs to discovery first, has enlarged the new medicine source of Radix Et Caulis Acanthopanacis Senticosi simultaneously.
Description of drawings
Fig. 1 is each treated animal TC, TG, LDL-cho, HDL-cho content and TC/HDL-cho ratio.
The specific embodiment
The present invention can further specify by following experimental example.
Experimental example 1, Radix Acanthopanacis Senticosi fruit water extract are tested the influence of hyperlipemia in mice
One, materials and methods
1.1 animal
The ICR mice, 70, male 50, female 20, body weight 18-20g.Available from preclinical medicine institute of Jilin University zoopery center, the animal quality certification number: SCXK-(Ji)-2003-0001.
1.2 medicine and reagent
XUEZHIKANG JIAONANG, specification: the 300mg/ grain, lot number 20060305, the Beijing WBL Peking University Biotech Co., Ltd produces;
The fenofibrate group, specification: the 200mg/ grain, lot number 89396, French Li Bofuni drugmaker produces;
Cholesterol, specification: biochemical reagents, the 25g/ bottle, lot number: 070516, Tianjin recovery fine chemistry industry institute;
Sodium cholate, specification: biochemical reagents, lot number: 20070525, Beijing chemical reagents corporation;
Adeps Sus domestica is provided by this research department;
Serum total cholesterol (TC) assay test kit, lot number: 070501, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
Triglyceride (TG) assay test kit, lot number: 071831, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
HDL-C (HDL-cho) assay test kit, lot number: 070171, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
Low-density lipoprotein cholesterol (LDL-cho) assay test kit, lot number: 070171, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.'s product.
The Radix Acanthopanacis Senticosi fruit water extract, character: brown ceramic powder, acrid in the mouth, little hardship, lot number: 071015, regenerative medicine Science Institute of Jilin University provides.
1.3 instrument
Microplate reader, model: PECAN A-5082, Austria SUNRISE company product.
1.4 method
Radix Acanthopanacis Senticosi fruit water extract preparation method: get Radix Et Caulis Acanthopanacis Senticosi sarcocarp soaking object, use filtered through gauze, dispel the sarcocarp residue, leave and take filtrate, the heating concentrated filtrate gets Radix Acanthopanacis Senticosi fruit water extract extractum, and vacuum drying is ground into dried cream powder promptly.
High lipid food compound method: reference literature (Li Yikui, Wang Qinmao, Zhou Jinhuang. the herbal pharmacology experimental methodology. Shanghai: the .1991 of Shanghai science tech publishing house, the 1st edition: 398), in 88.7% normal feedstuff, add 10% Adeps Sus domestica, 1% cholesterol and 0.3% sodium cholate.Adeps Sus domestica heated dissolve, add cholesterol and sodium cholate, pour in the feedstuff after stirring, mix thoroughly, add an amount of warm water and mix thoroughly, be tiled in the square plate, the about 1.5cm of thickness is divided into the fritter of 2cm * 3cm size with the wallpaper cutter, places in 80 ℃ of thermostatic drying chambers and dries.
Dosage: calculate requirement according to each group dosage, the weight of animals, administration natural law respectively, the accurate title, decide, add a small amount of distilled water earlier, grind evenly, adding distil water to desired concn gets final product again, wherein, XUEZHIKANG JIAONANG group: 12mg/ml, fenofibrate group: 2mg/ml, Radix Acanthopanacis Senticosi fruit water extract high dose group: 20mg/ml, middle dosage group: 10mg/ml, low dose group: 5mg/ml.
Conformed after animal is bought 3 days, and be divided at random: the normal control group by body weight; The hyperlipidemia model group; Positive control drug XUEZHIKANG JIAONANG group (240mg/kg); Positive control drug fenofibrate group (40mg/kg); Radix Acanthopanacis Senticosi fruit water extract high dose group (400mg/kg), middle dosage group (200mg/kg) and low dose group (100mg/kg).Female 2 every group, male 8 every group.
In the experimentation, the normal control treated animal is raised with conventional feed, and other each treated animals are all raised with high lipid food.Laboratory animal every morning gastric infusion 1 time.Irritate the long-pending 20ml/kg that is of body of stomach.Normal control group and hyperlipidemia model treated animal are irritated the stomach distilled water, and the administration group gives corresponding drug solution respectively.In 2 weeks of successive administration, on last 1 administration that night, the animal fasting be can't help water 16 hours, and next day, eyeball was got blood 0.5ml, centrifugal (3000r/min) separation of serum; Adopt enzymic colorimetric to measure serum total cholesterol (TC) content, adopt enzymic colorimetric to measure serum triglycerides (TG) content, adopt phosphotungstic acid-magnesium precipitate method to measure serum high-density LP cholesterol (HDL-cho) content, adopt the polyvinyl sulfuric acid sedimentation method to measure serum low-density LP cholesterol (LDL-cho) content.
Achievement data is added and subtracted standard deviation with mean
Expression, t-check between statistical procedures method employing group.
Two, result and discussion
Experimental result shows, mice is after raising with 2 weeks of high lipid food modeling continuously, the serum TC of model group animal, TG, LDL-cho content all obviously raise than normal matched group, TC content normal control group is 3.16 ± 0.48mmol/L, model group is 6.54 ± 0.84mmol/L (comparing p＜0.001 with normal group); TG content normal control group is 0.69 ± 0.20mmol/L, and model group is 0.85 ± 0.21mmol/L (comparing p＜0.05 with normal group); LDL-cho content normal control group is 0.30 ± 0.28mmol/L, and model group is 1.92 ± 1.09mmol/L (comparing p＜0.01 with normal group); The serum hdl of model group animal-cho content obviously reduces, and the normal control group is 3.90 ± 1.63mmol/L, and model group is 2.40 ± 0.79mmol/L (comparing p＜0.05 with normal group); The ratio of serum TC of model group animal and HDL-cho content (TC/HDL-cho) obviously raises, and the normal control group is 0.90 ± 0.24, and model group was 2.89 ± 1.53 (comparing p＜0.01 with normal group); The result shows the establishment of hyperlipidemia animal model.
After the two weeks, Radix Acanthopanacis Senticosi fruit water extract 400mg/kg dosage (CWJS-H) group, (5.47 ± 1.16mmol/L) are starkly lower than hyperlipidemia model group (6.54 ± 0.84mmol/L) (comparing p＜0.05 with model group) to animal serum TC content; ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.01) to 0.58 ± 0.14mmol/L) also higher fat model group to serum TG content.
Radix Acanthopanacis Senticosi fruit water extract 200mg/kg dosage (CWJS-M) group, animal serum TC content (5.20 ± 1.61mmol/L) with the hyperlipidemia model group (6.54 ± 0.84mmol/L) obviously reduce significant difference (p＜0.05); ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.05) to 0.63 ± 0.17mmol/L) also higher fat model group to serum TG content.
Radix Acanthopanacis Senticosi fruit water extract 100mg/kg dosage (CWJS-L) group, ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.05) to 0.64 ± 0.25mmol/L) also higher fat model group to serum TG content.
Each treated animal TC, TG, LDL-cho, HDL-cho content and TC/HDL-cho ratio see Table 1, Fig. 1.
Table 1 Radix Acanthopanacis Senticosi fruit water extract is to the influence of experimental hyperlipidemia mice lipids contents
Compare with normal group,
#P＜0.05;
##P＜0.01;
###P＜0.001.Compare with model group,
*P＜0.05;
*P＜0.01.
Result of the test shows that the Radix Acanthopanacis Senticosi fruit water extract can obviously reduce hyperlipidemia model mice serum TC, TG content.But hyperlipidemia model mice serum HDL-cho, LDL-cho content there is not obvious influence.
Experimental example 2, Radix Acanthopanacis Senticosi fruit total saponins are tested the influence of hyperlipemia in mice
One, materials and methods
1.1 animal
The ICR mice, 80, male 60, female 20, body weight 18-20g.Available from preclinical medicine institute of Jilin University zoopery center, the animal quality certification number: SCXK-(Ji)-2003-0001.
1.2 medicine and reagent
XUEZHIKANG JIAONANG, specification: the 300mg/ grain, lot number 20060305, the Beijing WBL Peking University Biotech Co., Ltd produces;
The fenofibrate group, specification: the 200mg/ grain, lot number 89396, French Li Bofuni drugmaker produces;
Cholesterol, specification: biochemical reagents, the 25g/ bottle, lot number: 070516, Tianjin recovery fine chemistry industry institute;
Sodium cholate, specification: biochemical reagents, lot number: 20070525, Beijing chemical reagents corporation;
Adeps Sus domestica is provided by this research department;
Serum total cholesterol (TC) assay test kit, lot number: 070501, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
Triglyceride (TG) assay test kit, lot number: 071831, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
HDL-C (HDL-cho) assay test kit, lot number: 070171, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.;
Low-density lipoprotein cholesterol (LDL-cho) assay test kit, lot number: 070171, Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.'s product.
The Radix Acanthopanacis Senticosi fruit total saponins, character: brown ceramic powder, it is little to distinguish the flavor of, acrid in the mouth, little hardship, lot number: 071028 regenerative medicine Science Institute of Jilin University provides.
1.3 instrument
Microplate reader, model: PECAN A-5082, Austria SUNRISE company product.
1.4 method
Radix Acanthopanacis Senticosi fruit total saponins preparation method: with Radix Et Caulis Acanthopanacis Senticosi sarcocarp soaking object, use filtered through gauze, dispel the sarcocarp residue, leave and take filter, merging filtrate, filtrate macroporous adsorptive resins AB-8 is washed to effluent and does not develop the color, and uses 90% ethanol elution instead to not showing the saponin reaction, collect 90% ethanol elution, decompression recycling ethanol is to thick paste, and vacuum drying is ground into dried cream powder promptly.
High lipid food compound method: reference literature (Li Yikui, Wang Qinmao, Zhou Jinhuang. the herbal pharmacology experimental methodology. Shanghai: the .1991 of Shanghai science tech publishing house, the 1st edition: 398), in 88.7% normal feedstuff, add 10% Adeps Sus domestica, 1% cholesterol and 0.3% sodium cholate.Adeps Sus domestica heated dissolve, add cholesterol and sodium cholate, pour in the feedstuff after stirring, mix thoroughly, add an amount of warm water and mix thoroughly, be tiled in the square plate, the about 1.5cm of thickness is divided into the fritter of 2cm * 3cm size with the wallpaper cutter, places in 80 ℃ of thermostatic drying chambers and dries.
Dosage: calculate requirement according to each group dosage, the weight of animals, administration natural law respectively, the accurate title, decide, add a small amount of distilled water earlier, grind evenly, adding distil water to desired concn gets final product again, wherein, XUEZHIKANG JIAONANG group: 12mg/ml, fenofibrate group: 2mg/ml, Radix Acanthopanacis Senticosi fruit total saponins high dose group: 20mg/ml, middle dosage group: 10mg/ml, low dose group: 5mg/ml.
Conformed after animal is bought 3 days, and be divided at random: the normal control group by body weight; The hyperlipidemia model group; Positive control drug XUEZHIKANG JIAONANG group (240mg/kg); Positive control drug fenofibrate group (40mg/kg); Radix Acanthopanacis Senticosi fruit total saponins high dose group (400mg/kg), middle dosage group (200mg/kg) and low dose group (100mg/kg); Totally 10 groups, 10 every group.Female 2 every group, male 8 every group.
In the experimentation, the normal control treated animal is raised with conventional feed, and other each treated animals are all raised with high lipid food.Laboratory animal every morning gastric infusion 1 time.Irritate the long-pending 20ml/kg that is of body of stomach.Normal control group and hyperlipidemia model treated animal are irritated the stomach distilled water, and the administration group gives corresponding drug solution respectively.In 2 weeks of successive administration, on last 1 administration that night, the animal fasting be can't help water 16 hours, and next day, eyeball was got blood 0.5ml, centrifugal (3000r/min) separation of serum; Adopt enzymic colorimetric to measure serum total cholesterol (TC) content, adopt enzymic colorimetric to measure serum triglycerides (TG) content, adopt phosphotungstic acid-magnesium precipitate method to measure serum high-density LP cholesterol (HDL-cho) content, adopt the polyvinyl sulfuric acid sedimentation method to measure serum low-density LP cholesterol (LDL-cho) content.
Achievement data is added and subtracted standard deviation with mean
Expression, t-check between statistical procedures method employing group.
Two, result and discussion
Experimental result shows, mice is after raising with 2 weeks of high lipid food modeling continuously, the serum TC of model group animal, TG, LDL-cho content all obviously raise than normal matched group, TC content normal control group is 3.16 ± 0.48mmol/L, model group is 6.54 ± 0.84mmol/L (comparing p＜0.001 with normal group); TG content normal control group is 0.69 ± 0.20mmol/L, and model group is 0.85 ± 0.21mmol/L (comparing p＜0.05 with normal group); LDL-cho content normal control group is 0.30 ± 0.28mmol/L, and model group is 1.92 ± 1.09mmol/L (comparing p＜0.01 with normal group); The serum hdl of model group animal-cho content obviously reduces, and the normal control group is 3.90 ± 1.63mmol/L, and model group is 2.40 ± 0.79mmol/L (comparing p＜0.05 with normal group); The ratio of serum TC of model group animal and HDL-cho content (TC/HDL-cho) obviously raises, and the normal control group is 0.90 ± 0.24, and model group was 2.89 ± 1.53 (comparing p＜0.01 with normal group); The result shows the establishment of hyperlipidemia animal model.
After the two weeks, Radix Acanthopanacis Senticosi fruit total saponins 400mg/kg dosage (CWJG-H) group, (5.67 ± 0.79mmol/L) are starkly lower than hyperlipidemia model group (6.54 ± 0.84mmol/L) (p＜0.05) to animal serum TC content; ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.01) to 0.56 ± 0.20mmol/L) also higher fat model group to serum TG content.
Radix Acanthopanacis Senticosi fruit total saponins 200mg/kg dosage (CWJG-M) group, (5.27 ± 1.11mmol/L) obviously reduce marked difference (p＜0.05) with the hyperlipidemia model group to animal serum TC content; ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.01) to 0.55 ± 0.20mmol/L) also higher fat model group to serum TG content.
Radix Acanthopanacis Senticosi fruit total saponins 100mg/kg dosage (CWJG-L) group, ((0.85 ± 0.21mmol/L) obviously reduces (p＜0.05) to 0.54 ± 0.34mmol/L) also higher fat model group to animal serum serum TG content.
Each treated animal TC, TG, LDL-cho, HDL-cho content and TC/HDL-cho ratio see Table 2, Fig. 1.
Table 2 Radix Acanthopanacis Senticosi fruit total saponins is to the influence of experimental hyperlipidemia mice lipids contents
Compare with normal group,
#P＜0.05;
##P＜0.01;
###P＜0.001.Compare with model group,
*P＜0.05;
*P＜0.01.
Result of the test shows that the Radix Acanthopanacis Senticosi fruit total saponins can obviously reduce hyperlipidemia model mice serum TC, TG content.Wherein the reduction TC of Radix Acanthopanacis Senticosi fruit total saponins, TG content effect are better than the Radix Acanthopanacis Senticosi fruit water extract.But hyperlipidemia model mice serum HDL-cho, LDL-cho content there is not obvious influence.
The embodiment one of preparation medicament:
Radix Acanthopanacis Senticosi fruit water extract 250.0g adds starch 50g, and the hard softgel shell of packing into behind the mixing is made 1000, every 0.3g.
The embodiment two of preparation medicament:
Radix Acanthopanacis Senticosi fruit water extract 150.0g adds starch 50g, and the hard softgel shell of packing into behind the mixing is made 1000, every 0.2g.
The embodiment three of preparation medicament:
Radix Acanthopanacis Senticosi fruit total saponins 150.0g adds starch 150g, and the hard softgel shell of packing into behind the mixing is made 1000, every 0.3g.
The embodiment four of preparation medicament:
Radix Acanthopanacis Senticosi fruit total saponins 200.0g adds starch 100g, and the hard softgel shell of packing into behind the mixing is made 1000, every 0.3g.
The embodiment five of preparation medicament:
Radix Acanthopanacis Senticosi fruit water extract 150.0g, Radix Acanthopanacis Senticosi fruit total saponins 100.0g adds starch 50g, and the hard softgel shell of packing into behind the mixing is made 1000, every 0.3g.
Claims (4)
1. Radix Acanthopanacis Senticosi fruit water extract or Radix Acanthopanacis Senticosi fruit total saponins or Radix Acanthopanacis Senticosi fruit water extract and the Radix Acanthopanacis Senticosi fruit total astragalin composition application in preparation treatment hyperlipidemia medicine, health product or food additive; Wherein, the Radix Acanthopanacis Senticosi fruit water extract is meant that Radix Acanthopanacis Senticosi fruit obtains extractum through water extraction, filtration, after concentrating; The Radix Acanthopanacis Senticosi fruit total saponins is meant Radix Acanthopanacis Senticosi fruit behind water extraction, and extracting solution is crossed macroporous adsorbent resin, and water elution is used the 10%-95% ethanol elution instead then to colourless, collects ethanol elution, reclaims the part that ethanol obtains.
2. application according to claim 1, its application dose scope of Radix Acanthopanacis Senticosi fruit water extract or Radix Acanthopanacis Senticosi fruit total saponins or Radix Acanthopanacis Senticosi fruit water extract and Radix Acanthopanacis Senticosi fruit total astragalin composition is 20～500mg/ people/sky.
3. application according to claim 1, its application dose scope of Radix Acanthopanacis Senticosi fruit water extract or Radix Acanthopanacis Senticosi fruit total saponins or Radix Acanthopanacis Senticosi fruit water extract and Radix Acanthopanacis Senticosi fruit total astragalin composition is 30～200mg/ people/sky.
4. application according to claim 1 is characterized in that described medicine or health product are tablet, capsule, powder, drop pill, granule, syrup or chewing agent.
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| CN2008100507256A CN101584719B (en) | 2008-05-20 | 2008-05-20 | Application of acanthopanax fruit extractive in preparing medicament for treating hyperlipoidemia |
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| CN101584719B true CN101584719B (en) | 2011-11-09 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468860A (en) * | 2002-07-19 | 2004-01-21 | 哈尔滨誉衡药业有限公司 | Manyprickle acanthopanax general saponin extractive and its medicinal composition |
| CN101084952A (en) * | 2006-06-08 | 2007-12-12 | 天津天士力之骄药业有限公司 | Method for preparing extraction of acanthopanax senticousus saponins |
| CN101240004A (en) * | 2008-03-18 | 2008-08-13 | 山东大学威海分校 | Technique for preparing eleutheroside B |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468860A (en) * | 2002-07-19 | 2004-01-21 | 哈尔滨誉衡药业有限公司 | Manyprickle acanthopanax general saponin extractive and its medicinal composition |
| CN101084952A (en) * | 2006-06-08 | 2007-12-12 | 天津天士力之骄药业有限公司 | Method for preparing extraction of acanthopanax senticousus saponins |
| CN101240004A (en) * | 2008-03-18 | 2008-08-13 | 山东大学威海分校 | Technique for preparing eleutheroside B |
Non-Patent Citations (2)
| Title |
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| 张晶等.刺五加化学成分及药理学研究进展.《中国野生植物资源》.2008,第27卷(第2期), * |
| 杨春花等.刺五加的研究进展.《人参研究》.2004,(第1期), * |
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