CN101581690A - Special electrode for in-vivo voltammetry analysis and preparation method thereof - Google Patents
Special electrode for in-vivo voltammetry analysis and preparation method thereof Download PDFInfo
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- CN101581690A CN101581690A CNA200810111625XA CN200810111625A CN101581690A CN 101581690 A CN101581690 A CN 101581690A CN A200810111625X A CNA200810111625X A CN A200810111625XA CN 200810111625 A CN200810111625 A CN 200810111625A CN 101581690 A CN101581690 A CN 101581690A
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Abstract
The invention discloses a special electrode for in-vivo voltammetry analysis and a preparation method thereof. The preparation method of the special electrode comprises the following steps: 1) preparing carbon fiber coated with a carbon nanomaterial on surface; 2) preparing a carbon fiber microelectrode; 3) soaking the carbon fiber electrode prepared by the step 2) in solution for ultrasonic washing; and 4) subjecting the carbon fiber electrode prepared by the step 3) to electrochemical pretreatment to obtain the special electrode. Meanwhile, an electrochemical detection device for in vitro correction or in vivo real-time physiological activator monitoring is prepared by using the special electrode. The electrochemical detection device comprises the special electrode for physiological activator monitoring of the invention, a reference electrode and an auxiliary electrode. The electrochemical detection device prepared by using the special electrode of the invention can meet the requirements for the in-vivo voltammetry analysis of physiological activators and has a promising application prospect in researches in fields of neurophysiology, clinical medicine and the like.
Description
Technical field
The present invention relates to a kind of electrode special that can satisfy the physiological activator vivo voltammetry analysis and preparation method thereof.
Background technology
In the last few years, chemical substance Study of variation law in physiology, pathologic process had become the advanced problems of people's extensive concern, and this mainly is because the information of chemical substance live body real-time change can directly reflect the chemical nature in physiology and the pathologic process.For example, the variation of glucose and lactic acid can directly reflect brain self-energy metabolic process in the brain; Neurotransmitter dopamine exists level and Changing Pattern thereof directly related with important physiological phenomenons such as somatic movement and cerebrations in brain; The oxidative damage that has level and interior free yl and the synthetic of neuropeptide of ascorbic acid are closely related in the brain.Therefore, foundation can be applicable to that the real-time monitoring method of live body of chemical substance in the biosome has crucial physiology and pathology sense.
Microelectrode vivo voltammetry method is because employed electrode has micron or nano-grade size and has advantages such as very high time and spatial resolution for biosome damage features of smaller and method itself, and the live body that is particularly suitable for physiological activator is monitored in real time.At present, miniature electrochemical is to utilize carbon fiber microelectrodes with micro pipette tips, carbon paste microelectrode, metal microelectrode and some modified electrode to set up and develop the detection method of physiological activator basically.Though can satisfying the live body of part physiological activator, the method for having set up monitors in real time, but still there is very big challenge in the research of microelectrode vivo voltammetry analysis method, is mainly reflected in aspects such as the reappearance of preparation, method of the electrode that can satisfy the vivo voltammetry analysis purpose and stability.
Summary of the invention
The purpose of this invention is to provide a kind of physiological activator live body that is used for and monitor electrode special of usefulness and preparation method thereof in real time.
Electrode special provided by the present invention prepares according to the method that may further comprise the steps:
1) carbon fiber of preparation surface coverage carbon nanomaterial;
2) carbon fiber with step 1) prepares carbon fiber microelectrodes with micro pipette tips;
3) with step 2) carbon fiber microelectrodes with micro pipette tips of preparation immerses in the solution and carries out ultrasonic cleaning;
4) carbon fiber microelectrodes with micro pipette tips of step 3) is carried out electrochemical pre-treatment, promptly obtain electrode special of the present invention.
Wherein, the carbon fiber of the surface coverage carbon nanomaterial of described step 1) is prepared as follows:
At first, with carbon fiber and SiCl
4At high temperature reaction obtains SiO
2The carbon fiber that covers, the Pintsch process by iron-phthalocyanine again is at SiO
2The uniform and stable carbon nanomaterial of carbon fiber surface growth that covers.(list of references: Small 2006,2,1052-1059 and The Journal of Physical Chemistry B 1999,103,4223-4227)
The carbon fiber microelectrodes with micro pipette tips of described surface coverage carbon nanomaterial can be prepared as follows:
The resulting carbon fiber of described step 1) is attached on the tinsel of conduction with conducting resinl, and penetrate in the glass capillary of the both ends open that has drawn, the glass tube front end exposes the carbon fiber of certain-length.Then the glass tube rear and front end is sealed with insulating gel, and prepared electrode is placed 100 ℃ of down baking 1h, can obtain step 2) carbon fiber microelectrodes with micro pipette tips of described surface coverage carbon nanomaterial.The tinsel of described conduction specifically can be copper wire or iron wire.
Ultrasonic cleaning in the described step 3) is successively at acetone, ethanol, 1.0～3.0M HNO
3Carry out in solution, 1.0～2.0M KOH solution and the secondary water.
The electrochemical pre-treatment of described step 4) can be carried out in acidic aqueous solution or alkaline aqueous solution; Described acidic aqueous solution specifically can be sulfuric acid solution or the 0.5M salpeter solution of 0.5M; Described alkaline aqueous solution specifically can be the NaOH solution of 0.5～1.0M, KOH solution or the 0.5～1.0M Ba (OH) of 0.5～1.0M
2Solution.
Electrochemical pre-treatment in the described step 4) can adopt cyclic voltammetry, potential step method or chronoamperometry.
The method of the electrochemical pre-treatment of described step 4) specifically can according to following a) or b) carry out:
A) carbon fiber microelectrodes with micro pipette tips with the surface coverage carbon nanomaterial of step 3) immerses in the sulfuric acid solution or 0.5M salpeter solution of 0.5M, applies earlier+potential polarization 30～50s of 2.0V, applies then-potential polarization 10～30s of 1.0V, again with 0.05～0.2Vs
-1Sweep velocity in the potential range of 0.0-1.0V scan round till obtain stable cyclic voltammogram;
B) carbon fiber microelectrodes with micro pipette tips of the surface coverage carbon nanomaterial of step 3) is immersed the NaOH solution of 1.0M, KOH solution or the 0.5～1.0M Ba (OH) of 1.0M
2In the solution, apply+potential polarization 50～180s of 1.0～1.5V.
Utilize electrode special of the present invention, can also prepare a kind of electrochemical detection device that live body is monitored physiological activator in real time that is used for, comprising: the present invention is used for electrode special, contrast electrode and the auxiliary electrode of physiological activator monitoring.
Described electrode special and electrochemical detection device can be used for external correction and live body is monitored physiological activator in real time; The physiological activator of described external correction is an adrenaline, and the described live body physiological activator of monitoring in real time is an ascorbic acid.
Carbon nanomaterial causes people's extensive interest owing to have unique physics and chemical property in a lot of scientific researches and industrial circle.Carbon nanomaterial and have important application by the nano-complex that the whole bag of tricks obtains in the research of Electroanalytical Chemistry is becoming the focus of people's extensive concern based on the Electroanalytical Chemistry research of carbon nanomaterial and nano-complex thereof.
The present invention utilize surface coverage the carbon fiber of uniform and stable carbon nanomaterial prepare microelectrode, by it is carried out easy pre-service, can obtain that physiological activator is had good electrochemical catalysis effect and the electrode special that satisfies the real-time volt-ampere analysis of physiological activator live body.This electrode special has been avoided carbon nanomaterial manually modified on the fine little surface of carbon, thereby has an advantage that is easy to reappear.In addition, this electrode special has very high selectivity, lower detectability and stability preferably, can satisfy the vivo voltammetry analysis of physiological activator.This is invented described electrode special and is with a wide range of applications in researchs such as neuro-physiology and clinical medicine.
Description of drawings
Fig. 1 is the synoptic diagram of the electrode special of embodiment 3 and embodiment 4; Wherein, 1 is copper wire, and 2 is glass capillary, and 3 is conducting resinl, and 4 is the carbon fiber of the surface coverage carbon nanomaterial of embodiment 1.
Fig. 2 is that the electrochemical detection device of embodiment 5 is to adrenergic voltammogram in the artificial cerebrospinal fluid.
Fig. 3 is the vivo voltammetry figure of the electrochemical detection device of embodiment 6 to ascorbic acid in the rat brain.
Embodiment
The preparation of the carbon fiber of embodiment 1, surface coverage carbon nanomaterial
Carbon fiber (T650-35, diameter are 7 μ m) (Fabric Development Inc.).Carbon fiber inserted in 1100 ℃ the high temperature furnace, and with SiCl
4The mixed airflow 20min that feeds 300mL/min argon gas and 40mL/min hydrogen injects the high temperature furnace that contains normal pressure oxygen, by utilizing SiCl
4With the reaction of carbon fiber, the surface fine little at carbon generates SiO
2Thin layer.With prepared carbon fiber and 0.1～0.3g iron-phthalocyanine (FeC
32N
8H
16) place same quartz glass tube respectively, at Ar/H
2(volume ratio is 1: 1～1: 2, and flow velocity is 20～40cm
3Under/min) the atmosphere, carry out different heat treated with iron-phthalocyanine for carbon is fine little.Fine little thermal treatment is to be 800～1100 ℃ of heating 2～15min down in temperature for carbon, is to be 500～600 ℃ of heating 2～15min down in temperature for the thermal treatment of iron-phthalocyanine.The temperature of controlling both subsequently is 800～1100 ℃ of heating 20～30min, can obtain the carbon fiber of surface coverage carbon nanomaterial.The diameter of used carbon fiber does not have obvious influence to this electrochemical catalysis performance of inventing described electrode special.(list of references: Small 2006,2,1052-1059 and The Journal of Physical Chemistry B1999,103,4223-4227)
The preparation of the carbon fiber microelectrodes with micro pipette tips of embodiment 2, surface coverage carbon nanomaterial
Attach on 5～10cm copper wire the carbon fiber of the surface coverage carbon nanomaterial of the embodiment 1 of 1～2cm is little with conducting resinl, and it is penetrated (internal diameter is 1.5mm in the glass capillary of the both ends open that has drawn, length is 10cm), thin end opening internal diameter is 30～50 μ m, and the fine little length of the carbon that this end exposes is about 1mm.The butt end of glass tube exposes copper wire.After the both ends open of glass tube was sealed with insulating gel, prepared electrode placed 100 ℃ of baking 1h down, promptly got the carbon fiber microelectrodes with micro pipette tips of surface coverage carbon nanomaterial.
1) carbon fiber electrode of the surface coverage carbon nanomaterial that embodiment 2 is prepared is successively at acetone, ethanol, 1.0～3.0M HNO
3, ultrasonic in 1.0～2.0M KOH solution and the secondary water.Ultrasound intensity is 20W/cm
2, ultrasonic frequency is 50kHz, and the mode of ultrasonication is the ultrasonic work 20s of every interval 15s, and the ultrasonic time is 2～5min;
2) the step 1) carbon fiber electrode is carried out electrochemical activation in the sulfuric acid solution of 0.5M, promptly at first on electrode, apply+potential polarization 30～50s of 2.0V, apply then-potential polarization 10～30s of 1.0V.Resulting electrode passes through the s with 0.05～0.2V again
-1The scan round of sweep velocity in the potential range of 0.0-1.0V arrive, till obtaining stable cyclic voltammogram.The prepared synoptic diagram that is used for physiological activator vivo voltammetry analysis electrode special as shown in Figure 1.
1) carbon fiber electrode of the surface coverage carbon nanomaterial that embodiment 2 is prepared is successively at acetone, ethanol, 1.0～3.0M HNO
3, ultrasonic in 1.0～2.0M KOH solution and the secondary water.Ultrasound intensity is 20W/cm
2, ultrasonic frequency is 50kHz, and the mode of ultrasonication is the ultrasonic work 20s of every interval 15s, and the ultrasonic time is 2～5min;
2) the step 1) carbon fiber electrode is carried out electrochemical activation in 0.5～1.0M NaOH solution, promptly on electrode, apply+1.0～+ current potential of 1.5V, polarization 50～180s.The prepared synoptic diagram that is used for physiological activator vivo voltammetry analysis electrode special as shown in Figure 1.
The external Electrochemical Detection of embodiment 5, physiological activator
The external electrochemical detection device of physiological activator comprises: the electrode special of embodiment 3 is a working electrode, and homemade Ag/AgCl (internal-filling liquid is an artificial cerebrospinal fluid) is a contrast electrode, and platinum filament is an auxiliary electrode.Document Analytical Chemistry 2007,79,6559-6565 are seen in the preparation of contrast electrode.This three electrode is inserted in the standard solution of certain density physiological activator and and be connected the voltammogram of record physiological activator with data collector with electrochemical workstation.This electrochemical workstation and data collector can adopt various equipment commonly used at present, such as electrochemical workstation BAS 100B/W be attached thereto the computer system that connects.
Fig. 2 is for utilizing above-mentioned electrochemical detection device to adrenergic external volt-ampere response diagram.The condition of cyclic voltammetric is: sweep limit is-0.1～+ 0.3V (vs.Ag/AgCl), sweep speed is 10mV/s, adrenergic concentration is 50 μ M.The result shows that prepared electrode special produces the current-responsive of stable state for adrenaline in artificial cerebrospinal fluid, and this current-responsive can be used for the external test of this material and proofread and correct adrenergic concentration in the animal body.
The vivo voltammetry of ascorbic acid monitoring in embodiment 6, the rat brain
The electrochemical detection device of physiological activator comprises: the electrode special of embodiment 4 is a working electrode, and homemade Ag/AgCl (internal-filling liquid is an artificial cerebrospinal fluid) is a contrast electrode, and platinum filament is an auxiliary electrode.Document Analytical Chemistry 2007,79,6559-6565 are seen in the preparation of contrast electrode.To be connected with data collector in the brain tissue of this three electrodes implantation rat and in electrochemical detection device.Galvanochemistry operation detection device station and data collector can adopt various equipment commonly used at present, such as electrochemical workstation BAS 100B/W be attached thereto the computer system that connects.
Fig. 3 utilizes the vivo voltammetry response diagram of above-mentioned detection device to ascorbic acid in the rat brain.Adopting the condition of live body cyclic voltammetric to be: sweep limit is-0.25～+ 0.2V (vs.Ag/AgCl), sweep velocity is 10mV/s.The result shows that electrode special prepared among the present invention produces tangible current-responsive to the ascorbic acid in the rat brain, can monitor the concentration of ascorbic acid in the animal brain in view of the above in real time.The species that have multiple electrochemical activity in rat brain, these species may produce current signal on electrode, and then disturb the mensuration of this method.Electrode special of the present invention has been realized its good electric catalysis and selective determination for ascorbic acid owing to the surface has carbon nanomaterial.The experiment that prepared electrode special is annotated ascorbic acid oxidase for the selectivity of ascorbic acid by slight irrigation in brain among the present invention has obtained further checking.Wherein, (Analytical Chemistry 2007,79,6559-6565) carry out by the middle method of describing according to document for the experimental technique of the interior slight irrigation notes of brain ascorbic acid oxidase.
Claims (10)
1, a kind of preparation method of microelectrode may further comprise the steps:
1) carbon fiber of preparation surface coverage carbon nanomaterial;
2) carbon fiber with step 1) prepares carbon fiber microelectrodes with micro pipette tips;
3) with step 2) carbon fiber microelectrodes with micro pipette tips of preparation immerses in the solution and carries out ultrasonic cleaning;
4) carbon fiber microelectrodes with micro pipette tips of step 3) is carried out electrochemical pre-treatment, promptly obtain described microelectrode.
2, method according to claim 1 is characterized in that: the carbon fiber of the surface coverage carbon nanomaterial in the described step 1) is prepared as follows: at first with carbon fiber and SiCl
4At high temperature reaction obtains SiO
2The carbon fiber that covers, the Pintsch process by iron-phthalocyanine again is at SiO
2The uniform and stable carbon nanomaterial of carbon fiber surface growth that covers.
3, method according to claim 1 and 2, it is characterized in that: the carbon fiber microelectrodes with micro pipette tips described step 2) is prepared as follows: attach to the resulting carbon fiber of described step 1) on the tinsel of conduction with conducting resinl, and penetrate in the glass capillary of the both ends open that has drawn, then the glass tube rear and front end is sealed with insulating gel, and prepared electrode placed 100 ℃ of down baking 1h, promptly obtain carbon fiber microelectrodes with micro pipette tips.
4, according to arbitrary described method among the claim 1-3, it is characterized in that: described ultrasonic cleaning is successively at acetone, ethanol, 1.0～3.0M HNO
3Carry out in solution, 1.0～2.0M KOH solution and the secondary water.
5, according to arbitrary described method among the claim 1-4, it is characterized in that: the electrochemical pre-treatment of described step 4) is carried out in acidic aqueous solution or alkaline aqueous solution; Described acidic aqueous solution is sulfuric acid solution or the 0.5M salpeter solution of 0.5M; Described alkaline aqueous solution is the NaOH solution of 0.5～1.0M, KOH solution or the 0.5～1.0M Ba (OH) of 0.5～1.0M
2Solution.
6, according to arbitrary described method among the claim 1-5, it is characterized in that: the electrochemical pre-treatment in the described step 4) can adopt cyclic voltammetry, potential step method or chronoamperometry;
Wherein, the method for electrochemical pre-treatment preferably according to following a) or b) carry out:
A) carbon fiber microelectrodes with micro pipette tips with the surface coverage carbon nanomaterial of described step 3) immerses in the sulfuric acid solution or 0.5M salpeter solution of 0.5M, applies earlier+potential polarization 30～50s of 2.0V, applies then-potential polarization 10～30s of 1.0V, again with 0.05～0.2V s
-1Sweep velocity in the potential range of 0.0-1.0V scan round till obtain stable cyclic voltammogram;
B) carbon fiber microelectrodes with micro pipette tips of the surface coverage carbon nanomaterial of described step 3) is immersed the NaOH solution of 1.0M, KOH solution or the 0.5～1.0M Ba (OH) of 1.0M
2In the solution, apply+potential polarization 50～180s of 1.0～1.5V.
7, the microelectrode for preparing according to arbitrary described method among the claim 1-6.
8, a kind of electrochemical detection device, comprising: the described microelectrode of claim 7, contrast electrode and auxiliary electrode.
9, described microelectrode of claim 7 or the described electrochemical detection device of claim 8 are monitored application in the physiological activator in real time at external correction or live body.
10, application according to claim 9 is characterized in that: the physiological activator of described external correction is an adrenaline, and the described live body physiological activator of monitoring in real time is an ascorbic acid.
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| CN102759554A (en) * | 2011-04-26 | 2012-10-31 | 电子科技大学 | Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters |
| CN106501331A (en) * | 2016-09-19 | 2017-03-15 | 中国科学院化学研究所 | PH sensors, preparation method and purposes |
| CN107271510A (en) * | 2017-06-14 | 2017-10-20 | 浙江理工大学 | Carbon fibres deposit metal phthalocyanine prepares electrochemical sensor |
| CN107865637A (en) * | 2016-09-28 | 2018-04-03 | 中国科学院化学研究所 | In vivo detection H2S electrode, preparation method and In vivo detection H2S device |
| CN107917948A (en) * | 2016-10-09 | 2018-04-17 | 中国科学院化学研究所 | In vivo detection electrode alignment method and its application |
| CN110031524A (en) * | 2019-02-21 | 2019-07-19 | 中国科学院化学研究所 | The method for measuring dopamine |
| CN110057897A (en) * | 2018-01-19 | 2019-07-26 | 中国科学院化学研究所 | The carbon nano tube modified carbon fiber electrode of electrophoretic deposition and its application in the detection of living body ascorbic acid |
| CN110389384A (en) * | 2019-06-18 | 2019-10-29 | 杭州电子科技大学 | Carbon fiber electrode and preparation method for low frequency ocean electric field detecting |
| CN111537576A (en) * | 2020-04-16 | 2020-08-14 | 中国科学院化学研究所 | Method for detecting neurochemical substance in brain |
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| CN1282870A (en) * | 1999-07-30 | 2001-02-07 | 武汉大学 | Process for preparing low-noise non-class carbon fibre electrode |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102759554A (en) * | 2011-04-26 | 2012-10-31 | 电子科技大学 | Manufacturing method of L-glutamic-acid-modified carbon fiber microelectrode, and application thereof in detecting neurotransmitters |
| CN106501331B (en) * | 2016-09-19 | 2019-10-11 | 中国科学院化学研究所 | PH sensor, preparation method and purposes |
| CN106501331A (en) * | 2016-09-19 | 2017-03-15 | 中国科学院化学研究所 | PH sensors, preparation method and purposes |
| CN107865637A (en) * | 2016-09-28 | 2018-04-03 | 中国科学院化学研究所 | In vivo detection H2S electrode, preparation method and In vivo detection H2S device |
| CN107917948B (en) * | 2016-10-09 | 2020-03-10 | 中国科学院化学研究所 | In vivo detection electrode correction method and application thereof |
| CN107917948A (en) * | 2016-10-09 | 2018-04-17 | 中国科学院化学研究所 | In vivo detection electrode alignment method and its application |
| CN107271510A (en) * | 2017-06-14 | 2017-10-20 | 浙江理工大学 | Carbon fibres deposit metal phthalocyanine prepares electrochemical sensor |
| CN110057897A (en) * | 2018-01-19 | 2019-07-26 | 中国科学院化学研究所 | The carbon nano tube modified carbon fiber electrode of electrophoretic deposition and its application in the detection of living body ascorbic acid |
| CN110057897B (en) * | 2018-01-19 | 2020-10-09 | 中国科学院化学研究所 | Carbon fiber electrode modified by electrophoretic deposition carbon nano tube and application thereof in detection of living ascorbic acid |
| CN110031524A (en) * | 2019-02-21 | 2019-07-19 | 中国科学院化学研究所 | The method for measuring dopamine |
| CN110389384A (en) * | 2019-06-18 | 2019-10-29 | 杭州电子科技大学 | Carbon fiber electrode and preparation method for low frequency ocean electric field detecting |
| CN110389384B (en) * | 2019-06-18 | 2020-12-22 | 杭州电子科技大学 | Carbon fiber electrode for low-frequency ocean electric field detection and preparation method thereof |
| CN111537576A (en) * | 2020-04-16 | 2020-08-14 | 中国科学院化学研究所 | Method for detecting neurochemical substance in brain |
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