CN101581654A - Method and device for obtaining volume information valid particle pulse and particle volume distribution - Google Patents
Method and device for obtaining volume information valid particle pulse and particle volume distribution Download PDFInfo
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- CN101581654A CN101581654A CNA2008100672785A CN200810067278A CN101581654A CN 101581654 A CN101581654 A CN 101581654A CN A2008100672785 A CNA2008100672785 A CN A2008100672785A CN 200810067278 A CN200810067278 A CN 200810067278A CN 101581654 A CN101581654 A CN 101581654A
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Abstract
The invention relates to a method and a device for obtaining a volume information valid particle pulse and a particle volume distribution, being applied to the field of particle detection. The method comprises the following steps: firstly obtaining the particle pulse of the particle to be detected passing through a detecting micropore and obtaining the volume information collecting point of the particle pulse, judging the validity of the particle pulse information through analyzing the running track information of the particle in the detecting micropore, i.e. identifying whether the particle pulse actually and effectively reflects the volume information of the corresponding particle or not, and deleting the particle pulse with greater distortion, and then reckoning all volume of the particle pulses with valid volume information into a particle volume distribution column diagram so as to obtain real and precise volume distribution information of the particle to be detected. The method further has strong adaptability and stability of a detecting result.
Description
Technical field
The present invention relates to the detection of particles analysis technical field, relate in particular to a kind of method and apparatus that adopts the micropore impedance principle to obtain effective particle pulse of volume information and the distribution of particle true volume.
Background technology
At present, in the particle analyzer of industrial particle analyzer, medical particle analyzer (comprising blood cell analyzer) and other purposes, adopt occupying the majority of micropore impedance principle.Based on the device for detecting particles principle of micropore impedance detection principle as shown in Figure 1, adopt the measuring process of the particle analyzer of micropore electrical impedance principle to be generally: particle to be measured is mixed with keeping liquid, make particle to be measured evenly be suspended in the testing liquid, form liquid to be measured; Utilize the liquid mobilization force to make liquid to be measured again by detecting micropore, the equivalent resistance that detects the micropore place when particle when detecting micropore changes, under the effect of micropore both sides constant current source, variation has taken place in the voltage of described detection micropore both sides, gather this change in voltage by Circuits System, the voltage pulse waveforms of formation shown in Fig. 1 b, these voltage pulse waveforms have just reflected that particle is by the impedance variation form in the micropore process.Generally,, characterized the volume information of particle, so particle analyzer can provide the volume distributed median situation of the particle that detects, the volume distributed median histogram of the particle of surveying promptly is provided because the height of this voltage pulse waveforms has reflected the volume size of particle.The histogrammic formation method of volume distributed median as shown in Figure 2, it is the respective channel of all detected particles being put into the particle volume distribution histogram according to the size of volume, it is passage (volume) that accumulation becomes a horizontal ordinate, and ordinate is the histogram of number of particles.
Because particle has the multiple path of passing through when detecting micropore, shown in Fig. 3 a, same particle is along illustrated A, B track process micropore, will produce two the different voltage pulse waveforms of " pulse A ", " pulse B " shown in Fig. 3 b respectively, according to usual way in each voltage pulse waveforms of Fig. 3 b, marked the detection volume point A that characterizes this particle volume size, the height of B visible detection volumetric point A, B increases successively, shows that the detection volume of pulse A, pulse B increases successively.This shows, when same particle passes through the path by the detection micropore with difference, can cause the distortion of particle pulse height, experimental results show that particle is from the most accurate near the particle volume of the potential pulse reflection that produces during by micropore on the track of central axis, particle trajectory is got over the off-center axis, untrue more according to the particle information that the potential pulse height obtains, therefore the validity of the particle volume that obtains respectively according to pulse A, pulse B then reduces successively.
All particle analyzers based on the micropore impedance principle all have the problems referred to above.Mainly contain three kinds of solutions at this problem at present: method 1, think that the particle volume error that this phenomenon causes is unimportant, ignore this influence, do not take any measure.This method can't obtain particle volume distributed intelligence accurately, this be mainly used in some to the particle volume requirement be not very strict or than low side instrument and equipment in.Method 2, physical method mechanical by increasing, fluid assembly form a specific velocity field at the micropore surveyed area, this flow field guarantees that all particles to be checked all pass through from the center of micropore, so just eliminated the formed pulse of track B shown in Fig. 3 a, guaranteed that detected information all can accurately reflect the true volume information of particle.This method can obtain particle volume distributed intelligence accurately, but needs to increase complicated mechanical, liquid current control parts and control method, has increased the cost of instrument.3, do not change physical unit, which particle detected signal is analyzed and identify is passed through from the micropore center, which particle passes through from the edge, when statistics detects distribution of particles information, only add up and be judged as the particle pulse signal that passes through from the micropore center, all the other particle pulse signals do not participate in statistics, can guarantee to obtain distribution of particles information accurately like this, this method has does not increase instrument cost, only handle and just can obtain distribution of particles information accurately from the analysis result of pulse signal, with the obvious advantage.
The prior art scheme is once belonging to the third method, its method is that each detected particle pulse is analyzed, find its first crest, with the primary peak position as boundary, result behind this pulse signal integration before this boundary is designated as A, result behind this pulse signal integration after this boundary is designated as B, two integrated signal A, B of pulse are compared, if it satisfies formula YB＜A＜ZB, think that then pulse information is effective, otherwise think that pulse information is invalid, wherein Y, Z are two constants.The core concept of this method is to judge that whether the symmetry before and after each pulse crest reaches predefined requirement, if reaching requirement thinks that then this pulse information is reliable, otherwise thinks that this pulse information is unreliable.But this scheme is only calculated the symmetry of pulse before and after the particle pulse signal primary peak, can't obtain reflection particle all information by the micropore track accurately, all sidedly, thereby can't judge the running orbit of particle more exactly; And because described method adopts the method for fixed constraints, can't answer the pulse signals feature because of variations that change produced such as instrument state, external environment, measurement particle characteristicses, therefore may cause a large amount of even all particles all are detected as effective particle, or all be detected as invalid particle, have stronger instability, have big defective.
Therefore, still there is defective in prior art, needs to improve.
Summary of the invention
Technical matters to be solved by this invention is: a kind of method and apparatus that adopts the micropore impedance principle to obtain the effective particle of volume information is provided.
Another purpose of the present invention is: provide a kind of micropore impedance principle that adopts to obtain the method and apparatus that the particle true volume distributes, thereby obtain truly, particle volume distributed intelligence to be measured accurately.
The present invention solves the problems of the technologies described above the technical scheme that is adopted to be:
A kind of method of obtaining the effective particle pulse of volume information may further comprise the steps:
A, collection particle pass through the pulse signal when detecting micropore under the effect of liquid stream;
B, described pulse signal is discerned, be used for determining characterizing the particle pulse of particle by the described detection micropore period, and the volume information collection point that obtains described particle pulse;
The reflection particle passes through the running orbit information that detects micropore in C, the described particle pulse of analysis, differentiates that whether described particle pulse reflects the volume of corresponding particle effectively, obtains the effective particle pulse of volume information.
A kind of method of obtaining the particle volume distribution may further comprise the steps:
A, collection particle pass through the pulse signal when detecting micropore under the effect of liquid stream;
B, described pulse signal is discerned, be used for determining characterizing the particle pulse of particle by the described detection micropore period, and the volume information collection point that obtains described particle pulse;
The reflection particle passes through the running orbit information that detects micropore in C, the described particle pulse of analysis, differentiates that whether described particle pulse reflects the volume of corresponding particle effectively, obtains the effective particle pulse of volume information.
D, utilize the effective particle pulse of described volume information, obtain the particle volume distributed intelligence.
A kind of device that obtains the effective particle pulse of volume information comprises: a signals collecting and processing module, be used to drive mix particles liquid to be measured by detecting micropore, and produce voltage signal, and described voltage signal is carried out the A/D conversion, form pulse signal; One pulse signal identification module is used for described pulse signal is discerned, and is set at the particle pulse signal with characterizing described particle to be measured by the pulse signal that detects the micropore period, and determines the volume information collection point of described particle pulse signal; Also comprise particle information validity identification module, be used for the motion track information of described example pulse signal is analyzed, differentiate that described particle pulse reflects the validity of corresponding particle volume, the effective particle pulse of output volume information.
A kind of device that obtains the distribution of particle true volume comprises: a signals collecting and processing module, be used to drive mix particles liquid to be measured by detecting micropore, and produce voltage signal, and described voltage signal is carried out the A/D conversion, the formation pulse signal; One pulse signal identification module is used for described pulse signal is discerned, and is set at the particle pulse signal with characterizing described particle to be measured by the pulse signal that detects the micropore period, and determines the volume information collection point of described particle pulse signal; And volume histogram generation module; Also comprise particle information validity identification module, be used for the motion track information of described example pulse signal is analyzed, differentiate that described particle pulse reflects the validity of corresponding particle volume, the effective particle pulse of output volume information; Described volume histogram generation module utilizes the effective particle pulse of all volume informations, forms the particle volume distribution histogram.
Beneficial effect of the present invention is: because the inventive method is conceived to particle is passed through the detection and the analysis of the movement locus when detecting micropore, come the validity of particle pulse information is judged by reflection particle information of running orbit in micropore in the extraction particle pulse waveform, judge from the basic reason that influences particle information validity whether particle information is effective, thereby obtain the effective particle of volume information, and particle volume distributed intelligence more really and accurately.
Description of drawings
Fig. 1 a is the device for detecting particles principle schematic based on micropore impedance detection principle;
Fig. 1 b is that particle is by detecting the voltage pulse waveforms synoptic diagram that micropore produces;
Fig. 2 is for forming the synoptic diagram of particle volume distribution histogram;
Fig. 3 a, b are respectively the synoptic diagram that produces different voltage pulse waveforms when particle to be measured passes through the detection micropore with different tracks;
Fig. 4 is for detecting liquid distributions synoptic diagram in the micropore;
Fig. 5 is for obtaining particle pulse signal volume information collection point synoptic diagram;
Fig. 6 detects synoptic diagram for first, second crossing time;
Fig. 7 a, b are respectively the first crossing time histogram and the second crossing time histogram of embodiment one;
Fig. 8 is that the effect after employing the inventive method compares synoptic diagram;
Fig. 9 a, b are respectively the first crossing time histogram and the second crossing time histogram of embodiment two;
Figure 10 is that total crossing time detects synoptic diagram;
Figure 11 is total crossing time histogram of embodiment three;
Figure 12 is total crossing time histogram of embodiment four;
Figure 13 obtains the method flow diagram that the particle true volume distributes for the present invention;
Figure 14 obtains the square law device block scheme that the particle true volume distributes for the present invention.
Embodiment
With embodiment the present invention is described in further detail with reference to the accompanying drawings below:
For obtain truly, particle volume distributed intelligence to be measured accurately, the present invention proposes a kind of according to particle pulse analysis obtain the method for the effective particle pulse of volume information, this method is: A, gather particle pass through pulse signal when detecting micropore under the effect of liquid stream; B, pulse signals are discerned, so that the particle pulse when obtaining particle to be measured by the detection micropore, and the volume information collection point that obtains particle pulse, the pulse height that certain of particle pulse signal is a bit located is represented the volume of corresponding particle, be directly proportional with the volume of particle, this point becomes the volume information collection point of particle pulse; C, by analysis to particle running orbit information in detecting micropore, judge the validity of particle pulse information, differentiate that promptly particle pulse reflects the volume information of corresponding particle whether authentic and validly, and reject the bigger particle pulse of distortion, obtain the effective particle pulse of volume information.Obtain the method for the effective particle pulse of volume information according to this, the invention allows for the method that the particle true volume distributes that obtains, promptly after step C, also comprise execution in step D, with all volumes that is judged as the effective particle pulse of volume information, obtain real particle volume distributed intelligence.For example count the particle volume distribution histogram, obtain particle volume distributed intelligence to be measured accurately with all volumes that are judged as the effective particle pulse of volume information.
According to aforementioned analysis as can be known, particle passes through the movement locus of micropore the closer to the micropore central axis, and the distortion of particle pulse sign particle volume information is more little, departs from the micropore central axis more otherwise particle passes through the movement locus of micropore, and its distortion is big more.According to fluid mechanics principle, the distribution of the flow stream velocity in the micropore is parabolic shape, and as shown in Figure 4, the flow rate of liquid on the micropore central axis is the fastest, and more away from the micropore central axis, flow rate of liquid is slow more, and the flow velocity at micropore edge is the slowest.Detecting liquid in the device for detecting particles is the suspending liquid of particle to be checked, and particle passes through when detecting micropore, and the distribution of its flow velocity is the highest in the middle of also being, the edge is minimum.Pass through at particle under this velocity flow profile of micropore, short more the closer to its time of passing through the detection micropore of particle of central axis, on the contrary then long more.This shows, the time that each particle to be measured passes through micropore can be reflected the running orbit when particle passes through micropore, therefore the present invention differentiates the size of particle pulse distorted signals by each particle being passed through detection and the analysis of micropore time, thereby judges the validity of particle pulse information.C step of the present invention preferably can comprise following step:
C1, by analysis to the waveform of particle pulse, determine that each particle to be measured runs to leave and detects the required crossing time of micropore from entering described detection micropore.The time that particle passes through micropore can bring into operation particle to and leaves the required time of micropore and be considered as a total crossing time from entering micropore, also total crossing time can be divided into two above periods, the crossing time that for example particle is passed through micropore is divided into 2 periods, be that particle enters the preceding half section process that micropore moves to place, volume information collection point that detect, continue to move to the second half section process that detects micropore of leaving from the volume information collection point with particle, and be first crossing time (HT_1st) with preceding half section procedure definition, the second half section procedure definition is second crossing time (HT_2nd), the running orbit of first crossing time and second crossing time these two parameters have been portrayed particle respectively before micropore half period and second half section is apart from the distance of micropore central axis, a relative total particle crossing time, therefore the running orbit that has reflected particle has more exactly improved the accuracy of differentiating particle.
C2, after obtaining the crossing time information of particle (the total crossing time or the one the second crossing times), the effective ratio of overall particle (HT_BothPerSet%) according to prior setting is carried out comprehensive dynamically judgement to the crossing time information that obtains, be identified for the decision condition of the effective particle of judgement information, and judge whether particle pulse information is effective, it is the volume information effective impulse that the particle pulse that satisfies above-mentioned qualifications is regarded as.。
At last all are satisfied the particle volume information that the effective particle pulse of volume information of decision condition characterized and add up, count the particle volume distribution histogram, detected the volume distributed median of particle accurately.Therefore can not satisfy the particle pulse of above qualifications, the particle volume of its sign then has been considered to than the large deviation distortion, disallowablely falls, and is not counted in the particle volume distribution histogram.
The flow process of the inventive method as shown in figure 13, embodiment one passes through the crossing time that detects micropore with particle to be measured to divide two periods into, i.e. first crossing time (HT_1st) and second crossing time (HT_2nd), embodiment one comprises following concrete steps:
A) if particle pulse has only a crest, as the situation of pulse A among Fig. 5, then 1. the crest location of pulse is the volume information collection point;
B) surpass a crest if particle pulse has, as the situation of pulse B among Fig. 5, then 1. the volume information collection point is the wave trough position between first crest and second crest.
A) crossing time of setting when prepulse detects height (HT_H) H, and this crossing time detects height less than place, particle pulse volume information collection point pulse height.Concrete establishing method is:
Crossing time detects height H=current pulse volume information gathering point place pulse height * certain prior preset proportion (height coefficient);
B) the 1. search pulse signal forward from the volume information collection point of particle pulse, the pairing moment of pulse signal that first that searches is equal to or less than crossing time detection height is designated as very first time point s1, and 3. the corresponding time period between the s0 constantly with current pulse volume information gathering point of very first time point s1 is first crossing time;
C) the 1. search pulse signal backward from the volume information collection point of particle pulse, the pairing moment of pulse signal that first that searches is equal to or less than crossing time detection height is designated as the second time point s2, and 4. the corresponding time period between the s0 constantly with current pulse volume information gathering point of the second time point s2 is second crossing time.
Wherein, the height coefficient of setting is answered the suitable setting of viewing system situation in advance, as detect that micropore varies in size, micropore inner structure difference, flow rate of liquid difference, measure particle and whether pass through special processing etc., in these cases, should determine and the height coefficient value of system's adaptation that suggestion height coefficient value is 0.1～0.8 according to experiment.
A) particle that all are recognized is accumulated according to its first crossing time, form first crossing time and number of particles corresponding relation, for example a width of cloth horizontal ordinate is first crossing time, and ordinate is the histogram of number of particles, be called the first crossing time histogram, referring to Fig. 7 a;
B) particle that all are recognized is accumulated according to its second crossing time, form second crossing time and number of particles corresponding relation, for example a width of cloth horizontal ordinate is second crossing time, and ordinate is the histogram of number of particles, be called the second crossing time histogram, referring to Fig. 7 b;
C) respectively in default two boundaries on first, second crossing time histogram: promptly on the first crossing time histogram, set the high limit of first crossing time, on the second crossing time histogram, set the high limit of second crossing time, and the high limit of first, second crossing time that will set is as the decision condition of differentiating the effective particle pulse of volume information.Because the length of particle crossing time to be measured is to determine by the track of the speed of liquid stream and particle is common, do not consider the difference of equipment and environment, sample factor, with the fixing crossing time upper limit as decision condition, then may cause in the detection of varying environment, sample the effective particle misjudgment phenomenon of information to occur, thereby lose adaptability environment, equipment and sample.Therefore the preferred embodiment for the present invention is: according to environment, equipment and sample factor, set the effective ratio of an overall particle in advance, according to the default effective ratio of overall particle the crossing time information that obtains is carried out comprehensive dynamically judgement, automatically adjust and finally set as first of the effective particle decision condition of judgement information, second crossing time high limit, the ratio that feasible satisfied simultaneously first crossing time is limit less than first crossing time height and second crossing time accounts for total detection total number of particles less than the high number of particles of limitting of second crossing time equals or the approaching effective ratio of overall particle (HT_BothPerSet%) of presetting.
Wherein, the default effective ratio of overall particle according to system design decision (as micropore size, flow velocity etc.), advises that this value is between 10%～90% by the user.By the establishing method of the effective particle ratio of information, can guarantee its environment, sample adaptability, improve the reliability of differentiating the effective particle pulse of volume information
Theoretically, the very little particle of first, second crossing time can not appear, because any particle all needs certain hour by detecting micropore.But in the actual implementation process, because error, the error of determining the volume information collection point and other error etc. of signal acquiring system may cause first, second crossing time of very small particles very little, so in order to get rid of disturbing factor, strengthen the reliability of this method in specific implementation process, in differentiating the effective particle pulse process of volume information, increased by two event horizons in the embodiment of the invention two.The embodiment of the invention two is as follows:
A) particle that all are recognized is accumulated according to its first crossing time, and forming a width of cloth horizontal ordinate is first crossing time, and ordinate is the histogram of number of particles, is called the first crossing time histogram, referring to Fig. 9 a;
B) particle that all are recognized is accumulated according to its second crossing time, and forming a width of cloth horizontal ordinate is second crossing time, and ordinate is the histogram of number of particles, is called the second crossing time histogram, referring to Fig. 9 b;
C) according to the effective ratio of setting in advance of default overall particle, respectively first, default four boundaries on the second crossing time histogram, shown in Fig. 9 a and 9b, promptly comparing with embodiment one increases by two event horizons: the first crossing time lower limit and the second crossing time lower limit, and the adjustment of four boundaries and setting will meet the following conditions, that is, make satisfied simultaneously first crossing time at the described first crossing time height, within the lower bound and second crossing time at the second crossing time height, the ratio that number of particles within the lower bound accounts for all total number of particles is similar to the described default effective ratio of overall particle.
First crossing time is greater than the first crossing time lower bound, and
First crossing time is limit less than first crossing time is high, and
Second crossing time is greater than the second crossing time lower bound, and
Second crossing time just is determined less than the pulse of the high limit of second crossing time and is called the effective particle pulse of volume information, the particle volume information of its sign is effective, these effective particle information are used for the statistics of integral particles volume information, it is unreliable that other is judged as its volume information of the invalid particle of information, is not used in the statistics of integral particles volume information.
Embodiments of the invention three are particle to be measured to be passed through the crossing time that detects micropore be considered as a complete procedure, and this embodiment compares embodiment one, two, has the advantage that is simple and easy to implement.Embodiment three comprises following concrete steps:
A) crossing time of setting when prepulse detects height (HT_H) H, and this crossing time detects height less than place, particle pulse volume information collection point pulse height.Concrete establishing method is identical with embodiment two with embodiment one:
Crossing time detects height H=current pulse volume information gathering point place pulse height * certain prior preset proportion (height coefficient);
B) the 1. search pulse signal forward from the volume information collection point of particle pulse is equal to or less than the pairing moment of pulse signal that crossing time detects height with first that searches and is designated as very first time point s1; The 1. search pulse signal backward from the volume information collection point of particle pulse, the pairing moment of pulse signal that first that searches is equal to or less than crossing time detection height is designated as the second time point s2, and 5. the time period that the second time point s2 put between the s1 to the very first time is total crossing time.
A) all particles are added up according to described crossing time and number of particles corresponding relation, formed the crossing time histogram, as shown in figure 11;
B) on the crossing time histogram, set the high limit of a crossing time, the setting of the high limit of this crossing time meets the following conditions, that is, make and satisfy total crossing time and be similar to the described default effective ratio of overall particle less than the ratio that the number of particles of the high limit of described crossing time accounts for all total number of particles.
Embodiments of the invention four are similar to embodiment three, and different is to have increased a crossing time lower limit in differentiating the effective particle pulse process of volume information, to strengthen the reliability of this method in specific implementation process.Embodiment four comprises following concrete steps:
A) all particles are added up according to described crossing time and number of particles corresponding relation, formed the crossing time histogram, as shown in figure 12;
B) on the crossing time histogram, set high limit of a crossing time and the high limit of a crossing time, the setting of high limit of this crossing time and lower bound meets the following conditions, that is, make and satisfy crossing time and be similar to the described default effective ratio of overall particle greater than described crossing time lower bound and less than the ratio that the number of particles of the high limit of described crossing time accounts for all total number of particles.
The present invention also provides and has obtained the device that the particle true volume distributes, and this installs as shown in figure 14, comprising: a signals collecting and processing module, be used to drive mix particles liquid to be measured by detecting micropore, produce voltage signal, and described voltage signal is carried out the A/D conversion, form pulse signal; One pulse signal identification module is used for described pulse signal is discerned, and is set at the particle pulse signal with characterizing described particle to be measured by the pulse signal that detects the micropore period, and determines the volume information collection point of described particle pulse signal; Also comprise particle information validity identification module, this module is used for the motion track information of particle pulse signal is analyzed, and differentiates whether particle pulse reflects the volume of corresponding particle effectively, and the effective particle pulse of output volume information; And the volume histogram generation module, described volume histogram generation module utilizes the effective particle pulse of all volume informations, forms the particle volume distribution histogram.Particle information validity identification module comprises pulse crossing time information detection module and the dynamic judge module of synthetic impulse, described pulse crossing time information detection module is used to detect and determines that each particle to be measured runs to leave and detects the required crossing time of micropore from entering described detection micropore, for example the crossing time branch is set as first crossing time and second crossing time after two periods, detect respectively and obtain first crossing time and second crossing time, and obtain total crossing time; The dynamic judge module of described synthetic impulse is used for setting decision condition according to the distributed intelligence of default effective ratio of overall particle and described crossing time, differentiates the validity of described particle pulse reflection particle volume, obtains the effective particle pulse of volume information.
Apparatus and method described in the invention are conceived to particle and pass through movement locus when detecting micropore, the symmetry of pulse signals does not add restriction, but come the validity of paired pulses information to be judged by reflection particle method of running orbit in micropore in the extraction pulse waveform, judge from the basic reason that influences particle information whether particle information is effective, thereby obtain particle volume distributed intelligence more accurately.Simultaneously, because the symmetry of paired pulses or time etc. are not done direct qualification to this method, but the method that adopts total effectively particle ratio to determine, thereby can adapt to the variation that instrument state, external environment variation, measurement particle characteristics etc. change generation, avoided prior art may cause a large amount of even all particles all are detected as effective particle, or all be detected as the instability of invalid particle, so the inventive method has the very strong adaptability and the stability of testing result.
The method of the invention proves truly feasible through experiment and checking.Can be applied in the blood cell analyzer, can obtain real more cell volume distribution histogram, for clinical diagnosis provides measurement result accurately.
Be understandable that, for those of ordinary skills, can be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, for example adopting same disposal route to carry out particle information validity for the pulse signal source of non-micropore impedance method judges, and utilize laser that particle is carried out scattered light when detecting, when certain feature that scattered signal is expressed particle detects, obtain particle characteristic information distribution situation accurately thereby also can utilize the method for the invention that its effectiveness of information is judged.And all these distortion or replacement all should belong to the protection domain of the appended claim of the present invention.
Claims (17)
1, a kind of method of obtaining the effective particle pulse of volume information is characterized in that, may further comprise the steps:
A, collection particle pass through the pulse signal when detecting micropore under the effect of liquid stream;
B, described pulse signal is discerned, be used for determining characterizing the particle pulse of particle by the described detection micropore period, and the volume information collection point that obtains described particle pulse;
The reflection particle passes through the running orbit information that detects micropore in C, the described particle pulse of analysis, differentiates that whether described particle pulse reflects the volume of corresponding particle effectively, obtains the effective particle pulse of volume information.
2, a kind of method of obtaining the particle volume distribution is characterized in that, may further comprise the steps:
A, collection particle pass through the pulse signal when detecting micropore under the effect of liquid stream;
B, described pulse signal is discerned, be used for determining characterizing the particle pulse of particle by the described detection micropore period, and the volume information collection point that obtains described particle pulse;
The reflection particle passes through the running orbit information that detects micropore in C, the described particle pulse of analysis, differentiates that whether described particle pulse reflects the volume of corresponding particle effectively, obtains the effective particle pulse of volume information.
D, utilize the effective particle pulse of described volume information, obtain the particle volume distributed intelligence.
3, method according to claim 2 is characterized in that: described step C comprises as follows:
The waveform of C1, the described particle pulse of analysis is used for determining that each particle to be measured runs to the crossing time that leaves the detection micropore from entering described detection micropore;
C2, according to the decision condition of differentiating the effective particle pulse of volume information each particle pulse is carried out validity and judge that the particle pulse that satisfies described decision condition is the effective particle pulse of volume information; Described decision condition is provided with according to the distributed intelligence of described particle crossing time to be measured.
4, method according to claim 3 is characterized in that: the described decision condition among the described step C2 also is provided with according to the default effective ratio of overall particle.
5, method according to claim 4, it is characterized in that: described crossing time comprises that particle to be measured runs to first crossing time of described volume information collection point and particle to be measured and moves to from described volume information collection point and leave second crossing time that detects micropore from entering described detection micropore.
6, method according to claim 5 is characterized in that: described first crossing time and second crossing time obtain in accordance with the following methods:
C111, on the oscillogram of particle pulse, search first along described particle pulse volume information collection point forward and be equal to or less than the pairing very first time point of pulse signal that default crossing time detects height, and search first along described particle pulse volume information collection point backward and be equal to or less than pairing second time point of pulse signal that described crossing time detects height, this crossing time detects the pulse height of height less than described volume information collection point;
C112, to obtain described volume information collection point corresponding be described first crossing time with mistiming of described very first time point constantly, and the mistiming in the corresponding moment with described volume information collection point of described second time point is described second crossing time.
7, method according to claim 6 is characterized in that: described crossing time detection highly equals place, current particle pulse volume information collection point pulse height and multiply by the predetermined altitude coefficient.
8, method according to claim 7 is characterized in that: described step C2 comprises as follows:
C211, all particles are added up according to described first crossing time and number of particles corresponding relation and second crossing time and number of particles corresponding relation respectively, form first crossing time and number of particles corresponding relation, and second crossing time and number of particles corresponding relation;
C212, make and satisfy described first crossing time simultaneously and be similar to the described default effective ratio of overall particle less than the ratio that the number of particles of the high limit of default second crossing time accounts for all total number of particles less than the high limit of default first crossing time and described second crossing time;
C213, each particle pulse is carried out validity judge, prescribe a time limit less than described second crossing time is high less than second crossing time of the high limit of described first crossing time and this particle pulse when first crossing time of particle pulse, then this particle pulse is the effective particle pulse of volume information.
9, method according to claim 6 is characterized in that: described step C2 comprises as follows:
C221, all particles are added up according to described first crossing time and number of particles corresponding relation and second crossing time and number of particles corresponding relation respectively, form first crossing time and number of particles corresponding relation, and second crossing time and number of particles corresponding relation;
C222, make satisfy simultaneously described first crossing time within the default high and low limit of first crossing time and the number of particles of described second crossing time within the default high and low limit of second crossing time ratio that accounts for all total number of particles be similar to the described default effective ratio of overall particle;
C223, each particle pulse is carried out validity judge, when first crossing time of particle pulse is limit greater than the described first crossing time lower bound and less than described first crossing time is high, second crossing time of this particle pulse is limit greater than the described second crossing time lower bound and less than described second crossing time is high simultaneously, and then this particle pulse is the effective particle pulse of volume information.
10, method according to claim 4, it is characterized in that: described crossing time obtains in accordance with the following methods: on the oscillogram of particle pulse, search first along described particle pulse volume information collection point forward and be equal to or less than the pairing very first time point of pulse signal that default crossing time detects height, and search first along described particle pulse volume information collection point backward and be equal to or less than pairing second time point of pulse signal that described crossing time detects height, this crossing time detects the pulse height of height less than described volume information collection point; Described crossing time equals the mistiming between described second time point and the point of the described very first time.
11, method according to claim 10 is characterized in that: described step C2 comprises as follows:
C231, all particles are added up according to described crossing time and number of particles corresponding relation, formed crossing time and number of particles corresponding relation;
C232, make and satisfy the ratio that the number of particles of described crossing time within the high limit of default crossing time account for all total number of particles and be similar to the described default effective ratio of overall particle;
C233, each particle pulse is carried out validity judge, when the crossing time of particle pulse is in limited time high less than described crossing time, then this particle pulse is the effective particle pulse of volume information.
12, method according to claim 10 is characterized in that: described step C2 comprises as follows:
C241, all particles are added up according to described crossing time and number of particles corresponding relation, formed crossing time and number of particles corresponding relation;
C242, make and satisfy described crossing time and be similar to the described default effective ratio of overall particle greater than default crossing time lower bound and less than the ratio that the number of particles of the high limit of described crossing time accounts for all total number of particles;
C243, each particle pulse is carried out validity judge, when the described crossing time of particle pulse during less than the high limit of described crossing time and greater than described crossing time lower bound, then this particle pulse is the effective particle pulse of volume information.
13, according to the described method of the arbitrary claim of claim 2 to 12, it is characterized in that: described particle pulse volume information collection point is determined in accordance with the following methods: the oscillogram to described particle pulse is analyzed,
When described particle pulse had only a crest, then the volume information collection point was the crest location of described particle pulse;
When described particle pulse has when surpassing one crest, then the volume information collection point is the wave trough position between first crest and second crest.
14, method according to claim 13 is characterized in that: particle volume distributed intelligence described in the described step D is represented with the particle volume distribution histogram.
15, a kind of device that obtains the effective particle pulse of volume information comprises: a signals collecting and processing module, be used to drive mix particles liquid to be measured by detecting micropore, and produce voltage signal, and described voltage signal is carried out the A/D conversion, form pulse signal; One pulse signal identification module is used for described pulse signal is discerned, and is set at the particle pulse signal with characterizing described particle to be measured by the pulse signal that detects the micropore period, and determines the volume information collection point of described particle pulse signal; It is characterized in that:
Also comprise particle information validity identification module, be used for the motion track information of described example pulse signal is analyzed, differentiate that described particle pulse reflects the validity of corresponding particle volume, the effective particle pulse of output volume information.
16, a kind of device that obtains the distribution of particle true volume comprises: a signals collecting and processing module, be used to drive mix particles liquid to be measured by detecting micropore, and produce voltage signal, and described voltage signal is carried out the A/D conversion, the formation pulse signal; One pulse signal identification module is used for described pulse signal is discerned, and is set at the particle pulse signal with characterizing described particle to be measured by the pulse signal that detects the micropore period, and determines the volume information collection point of described particle pulse signal; And volume histogram generation module; It is characterized in that:
Also comprise particle information validity identification module, be used for the motion track information of described example pulse signal is analyzed, differentiate that described particle pulse reflects the validity of corresponding particle volume, the effective particle pulse of output volume information; Described volume histogram generation module utilizes the effective particle pulse of all volume informations, forms the particle volume distribution histogram.
17, device according to claim 16, it is characterized in that: described particle information validity identification module comprises pulse crossing time information detection module and the dynamic judge module of synthetic impulse, described pulse crossing time information detection module is used for determining that each particle to be measured runs to leave and detects the required crossing time of micropore from entering described detection micropore, the dynamic judge module of described synthetic impulse is used for the distributed intelligence according to default effective ratio of overall particle and described crossing time, differentiate the validity of described particle pulse reflection particle volume, obtain the effective particle pulse of volume information.
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