CN101579264B - Device for preparing tubular bracket for tissue engineering for simulating in vivo tube-shaped organs - Google Patents
Device for preparing tubular bracket for tissue engineering for simulating in vivo tube-shaped organs Download PDFInfo
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- CN101579264B CN101579264B CN 200910099374 CN200910099374A CN101579264B CN 101579264 B CN101579264 B CN 101579264B CN 200910099374 CN200910099374 CN 200910099374 CN 200910099374 A CN200910099374 A CN 200910099374A CN 101579264 B CN101579264 B CN 101579264B
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- main body
- tissue engineering
- tubular bracket
- inner prop
- endoporus
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Abstract
The invention discloses a device for preparing a tubular bracket for the tissue engineering for simulating in vivo tube-shaped organs, which is characterized in that a main body is provided with an inner hole and comprises a left main body and a right main body that are arranged in a half-and-half way along the inner hole and fixedly connected with each other in a sealing way; the inner wall of the inner hole is stuck with a silastic plate, the inner hole is internally provided with an internal column in a coaxial way, concavo-convex pattern is arranged on one side of the silastic plate towards the internal column, and a gap is arranged between the silastic plate and the internal column; the device has the advantages that the growth and the differentiation of myocyte can be effectively induced and promoted, and micron pore structure inside the wall of the tube of the bracket also can cause epithelial cell/endothelial cell planted on the inner surface of the bracket to keep information intercommunication with myocyte and is beneficial to the exchange of nutrient substance and the discharge of excretion, so that the epithelial cell/endothelial cell which is in vitro culture can be ensured to maintain the respective intrinsical biological function, thus the artificial bracket constructed in vitro can be used for simulating the nature structure of the tube-shaped organs to the utmost extent.
Description
Technical field
The present invention relates to a kind of used in tissue engineering tubular bracket, relate in particular to the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body.
Background technology
This new branch of science of organizational project has obtained fast development in recent two decades, the research of many engineered organs and tissue has obtained very much progress, and one of most important technology is the preparation that is fit to the support of cell growth in the tissue engineering research.In human body, have many tubular tissues, like esophagus, intestine and small intestine and blood vessel etc., these tubular tissues have similar structure from microtexture; All forms by epithelial layer (in the blood vessel for endodermis) and flesh layer, between continuous by the conjunctive tissue that is rich in collagen, wherein epithelium/endodermis rises and protects and iris action; Stop content such as water, blood etc. to leak into body cavity, articulamentum and flesh layer are then kept the structural integrity of organ on the one hand, the more important thing is and have shunk and creeping effect; Make pipe performance respective action; Can food and water be transported to stomach from the oral cavity down at its wriggling like esophagus, and blood vessel can make blood flow unobstructed etc., and the epithelial cell in the tubular tissue (being endotheliocyte in the blood vessel) and myocyte's interaction; Exchange information, help cell performance biological function separately.The cell functions such as type looks, propagation, migration and differentiation that general structure pair cell is opened up on the surface of the verified support in order to sustenticular cell growth of scientific research have considerable influence; And proof significantly induces and facilitation the function tool of smooth muscle cell like the patterns such as sequence groove of certain size and the degree of depth, the still present also preparation facilities of this kind tubular bracket not.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation facilities of producing the used in tissue engineering tubular bracket of the natural structure of pipe in the analogue body; So that the support that makes can induce and promote myocyte's growth and differentiation on the one hand well; Support epithelium/endotheliocyte on rack inner wall, to grow simultaneously; Promote two types of cells to interact, exchange information, make the support of external structure can be on macroscopical profile and biological function more near body in tubular tissue.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body; It comprises base, main body and the cover plate of placing successively from bottom to up; Described main body is provided with endoporus; Described main body comprises left main body and the right main body that half-and-half is provided with and is sealedly and fixedly connected along described endoporus; The silicane rubber plate that has been sticked on the inwall of described endoporus is coaxially arranged with inner prop in the described endoporus, and described silicane rubber plate is provided with the concavo-convex pattern that promotes cell growth and differentiation towards the one side of described inner prop; Be provided with the annular gap that is used for primer solution between described silicane rubber plate and the described inner prop; Be provided with sealing device between described base and the described main body, be fixedly connected through long bolt between described base and the described cover plate, described cover plate is provided with the perfusing hole that is connected with described annular gap.
The bottom of described inner prop has been wholely set co-axial circular locating piece, and described base is fixedly connected with described locating piece, the diameter of described locating piece and the equal diameters of described endoporus.
The coaxial centring ring that is set with in the upper end of described inner prop; The diameter of described centring ring and the equal diameters of described endoporus; Described centring ring is provided with the cannelure that at least two equally distributed whiles are connected with described annular gap and described perfusing hole; During assembling, make endoporus and inner prop keep good concentricity, thereby make the tubular bracket wall thickness that makes even.
Described sealing device is first sealing strip that is arranged between the upper surface of bottom surface and described base of described main body; Be provided with second sealing strip between described left main body and the described right main body; Organic solvent to prevent to be poured in the annular gap is revealed; The material of described first sealing strip and described second sealing strip is silastic material, is corroded by organic solvent to prevent sealing strip.
Be provided with alignment pin between described left main body and the described right main body, guaranteed the accurate positioning when assembling of left main body and right main body.
One end of described long bolt is bolted with wing nut, and the other end of described long bolt passes described cover plate and described base is spirally connected, and has made things convenient for the fixing of main body, when main body cooperates closely with base, can pass through the wing nut adjusting.
Described perfusing hole is provided with dust cap, during support, can prevent that dust and other impurities from getting in the endoporus in preparation, and the polymer solution that also can prevent to be used for to produce support simultaneously is too fast in the operating process volatilization.
Described inner prop is provided with draw taper, with the demoulding from the inner prop of the convenient tubular bracket of being produced.
The material of described main body and described inner prop is metal or glass, in order to avoid this device is corroded by organic solvent.
Compared with prior art; Advantage of the present invention is because silicane rubber plate is provided with convex and concave patterns towards the one side of inner prop; Make the outer surface of made support also have corresponding pattern; Can induce and promote myocyte's growth and differentiation effectively, the micrometer grade hole structure of support tube pars intramuralis can impel the epithelium/endotheliocyte and the myocyte that plant on the support inner surface to keep information mutual communication again simultaneously, also helps the exchange and the excremental discharge of nutrient substance; Make the epithelium/endotheliocyte and the myocyte of In vitro culture keep original separately biological function, thereby the man-made support that makes external structure is from simulate the natural structure of pipe to the full extent; Again because main body comprises left main body and the right main body that half-and-half is provided with and is fixedly connected along endoporus; When making tubular bracket; Earlier left main body and right main body are half-and-half taken apart along endoporus, then the tubular bracket that makes is taken out from inner prop, when retracting, be damaged with the pattern that prevents rack outer surface.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 removes the vertical view behind the dust cap for the present invention;
Fig. 3 is the transverse sectional view (half-twist) of main body of the present invention;
Fig. 4 is the structural representation of centring ring of the present invention.
The specific embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
As shown in the figure, the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body, it comprises base 1, main body 2, cover plate 3, inner prop 5 and the long bolt of placing successively from bottom to up 8; Main body 2 is provided with endoporus 23, and main body 2 comprises the left main bodys 21 and right main body 22 that are provided with along 23 pairs half of endoporus, is provided with alignment pin 24 between left main body 21 and the right main body 22 and is connected through the screw crosswise fixed; Be provided with second sealing strip 25 between left side main body 21 and the right main body 22; Inner prop 5 is arranged in the endoporus 23, and inner prop 5 has certain draw taper, and the bottom of inner prop 5 has been wholely set co-axial circular locating piece 51; Base 1 and locating piece 51 are fixedly connected through screw; The equal diameters of the diameter of locating piece 51 and endoporus 23, the silicane rubber plate 4 that has been sticked on the inwall of endoporus 23, silicane rubber plate 4 is provided with the concavo-convex pattern (not shown) that promotes cell growth and differentiation towards the one side of inner prop 5; Be provided with the annular gap 6 that is used for primer solution between silicane rubber plate 4 and the inner prop 5; The upper end of inner prop 5 is through the coaxial centring ring 7 that is set with of screw, the equal diameters of the diameter of centring ring 7 and endoporus 23, and centring ring 7 is provided with four equally distributed cannelures 71; Cannelure 71 is connected with annular gap 6; Cover plate 3 is provided with the perfusing hole 31 that is connected with cannelure 71, and perfusing hole 31 is provided with dust cap 32, and an end of long bolt 8 is bolted with wing nut 81; The other end of long bolt 8 passes cover plate 3 and is spirally connected with base 1, is provided with first sealing strip 9 between the upper surface of the bottom surface of main body 2 and base 1.
A kind of operational approach to above-mentioned preparation facilities, its concrete steps are following:
(1), as requested prepare the silicon wafer template, and the pattern that the concavo-convex myocyte of promotion grows and breaks up is set on template;
(2), the pattern on the above-mentioned silicon wafer template is duplicated to softness but on the moderate silicane rubber plate 4 of intensity, and silicane rubber plate 4 usefulness double faced adhesive tapes are fixed in endoporus 23 surfaces of main body 2, and assemble this preparation facilities;
(3), dispose the polymer solution that is used to produce tubular bracket as requested;
(4), the polymer solution that configures is injected from perfusing hole 31 lentamente, the polymer solution after the injection is no more than the top of inner prop 5;
(5), according to the characteristic of polymer polymer solution being placed certain hour at a certain temperature makes it reach the microcosmic phase-splitting but the state of not phase-splitting on the macroscopic view;
(6), whole device was put into cryogenic refrigerator pre-cooled 1 hour;
(7), whole device was moved in the freezer dryer dry 24 hours.
The tubular bracket of producing through above-mentioned steps is not if there is bone dry; Then can the above-mentioned whole device that has tubular bracket be put into vacuum drying oven and continue dry 24 hours, guaranteeing the tubular bracket bone dry, when taking apart; Unclamp long bolt 8 earlier; Take off cover plate 3, laterally take left main body 21 and right main body 22 then apart, at last the tubular bracket that makes is taken out from inner prop 5.
In the foregoing description; The quantity of cannelure 71 also can be two, three etc., but must be uniform distribution, and can not be less than two; Also can use one through hole to substitute; The material of main body 2 and inner prop 5 is metals such as rustless steel, aluminum, also can be glass, and the material of first sealing strip 9 and second sealing strip 25 is silastic material.
Claims (7)
1. the preparation facilities of the used in tissue engineering tubular bracket of pipe in the analogue body; It comprises base, main body and the cover plate of placing successively from bottom to up; Described main body is provided with endoporus; Be provided with sealing device between described base and the described main body; Be fixedly connected through long bolt between described base and the described cover plate, it is characterized in that described main body comprises left main body and the right main body that half-and-half is provided with and is sealedly and fixedly connected along described endoporus, silicane rubber plate has been sticked on the inwall of described endoporus; Be coaxially arranged with inner prop in the described endoporus; The bottom of described inner prop has been wholely set co-axial circular locating piece, and described base is fixedly connected with described locating piece, the diameter of described locating piece and the equal diameters of described endoporus; Described silicane rubber plate is provided with the concavo-convex pattern that promotes cell growth and differentiation towards the one side of described inner prop; Be provided with the annular gap that is used for primer solution between described silicane rubber plate and the described inner prop, described cover plate is provided with the perfusing hole that is connected with described annular gap, the coaxial centring ring that is set with in the upper end of described inner prop; The diameter of described centring ring and the equal diameters of described endoporus, described centring ring are provided with the cannelure that at least two equally distributed whiles are connected with described annular gap and described perfusing hole.
2. the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body as claimed in claim 1; It is characterized in that described sealing device is first sealing strip that is arranged between the upper surface of bottom surface and described base of described main body; Be provided with second sealing strip between described left main body and the described right main body, the material of described first sealing strip and described second sealing strip is silastic material.
3. the preparation facilities of the used in tissue engineering tubular bracket of pipe is characterized in that being provided with alignment pin between described left main body and the described right main body in a kind of analogue body as claimed in claim 1.
4. the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body as claimed in claim 1; An end that it is characterized in that described long bolt is bolted with wing nut, and the other end of described long bolt passes described cover plate and described base is spirally connected.
5. the preparation facilities of the used in tissue engineering tubular bracket of pipe is characterized in that described perfusing hole is provided with dust cap in a kind of analogue body as claimed in claim 1.
6. the preparation facilities of the used in tissue engineering tubular bracket of pipe is characterized in that described inner prop is provided with draw taper in a kind of analogue body as claimed in claim 1.
7. the preparation facilities of the used in tissue engineering tubular bracket of pipe in a kind of analogue body as claimed in claim 1, the material that it is characterized in that described main body and described inner prop is metal or glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910099374 CN101579264B (en) | 2009-06-04 | 2009-06-04 | Device for preparing tubular bracket for tissue engineering for simulating in vivo tube-shaped organs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910099374 CN101579264B (en) | 2009-06-04 | 2009-06-04 | Device for preparing tubular bracket for tissue engineering for simulating in vivo tube-shaped organs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101579264A CN101579264A (en) | 2009-11-18 |
| CN101579264B true CN101579264B (en) | 2012-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200910099374 Expired - Fee Related CN101579264B (en) | 2009-06-04 | 2009-06-04 | Device for preparing tubular bracket for tissue engineering for simulating in vivo tube-shaped organs |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104013998B (en) * | 2014-05-26 | 2015-06-24 | 宁波大学 | Preparation method of artificial esophagus with histological structure |
| CN210656923U (en) * | 2019-09-11 | 2020-06-02 | 南通市肺科医院（南通市第六人民医院） | Anti-pollution tubercle bacillus culture tube |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762505A (en) * | 2005-08-11 | 2006-04-26 | 浙江大学 | Catheter stent preparation method for repairing tubular tissue and organ and apparatus thereof |
| US7192440B2 (en) * | 2003-10-15 | 2007-03-20 | Xtent, Inc. | Implantable stent delivery devices and methods |
| CN201208319Y (en) * | 2008-06-06 | 2009-03-18 | 宁波大学 | Preparation device of tubular bracket for tissue engineering |
-
2009
- 2009-06-04 CN CN 200910099374 patent/CN101579264B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192440B2 (en) * | 2003-10-15 | 2007-03-20 | Xtent, Inc. | Implantable stent delivery devices and methods |
| CN1762505A (en) * | 2005-08-11 | 2006-04-26 | 浙江大学 | Catheter stent preparation method for repairing tubular tissue and organ and apparatus thereof |
| CN201208319Y (en) * | 2008-06-06 | 2009-03-18 | 宁波大学 | Preparation device of tubular bracket for tissue engineering |
Non-Patent Citations (2)
| Title |
|---|
| Benjamin L. Beckstead等.Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering.《Biomaterials》.2005,6217-6228. * |
| 竺亚斌,刘玉新.热致相分离法制备食道组织工程用支架的研究.《宁波大学学报(理工版)》.2008,第21卷(第2期),240-245. * |
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| Publication number | Publication date |
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| CN101579264A (en) | 2009-11-18 |
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Granted publication date: 20120613 Termination date: 20200604 |