CN101576532A - Interface device for coupling capillary electrophoresis with inductively coupled plasma mass spectrograph and use method thereof - Google Patents
Interface device for coupling capillary electrophoresis with inductively coupled plasma mass spectrograph and use method thereof Download PDFInfo
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- CN101576532A CN101576532A CNA2009101120033A CN200910112003A CN101576532A CN 101576532 A CN101576532 A CN 101576532A CN A2009101120033 A CNA2009101120033 A CN A2009101120033A CN 200910112003 A CN200910112003 A CN 200910112003A CN 101576532 A CN101576532 A CN 101576532A
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Abstract
The invention provides an interface device for coupling a capillary electrophoresis with an inductively coupled plasma mass spectrograph and use method thereof. The interface device is characterized by comprising a stainless steel capillary pipe, a sample solution peristaltic pump and a T-shaped capillary pipe, wherein the stainless steel capillary pipe is connected with a high voltage source, an inlet on the left end thereof is respectively connected with the capillary electrophoresis and a miniflow and sheath flow pipe, and the output end of the stainless steel capillary pipe is connected with the input end of the T-shaped capillary pipe by the sample solution peristaltic pump; the output end of the T-shaped capillary pipe is connected in the inductively coupled plasma mass spectrograph; and the lower end of the T-shaped capillary pipe is connected with the output end of a wash solution peristaltic pump, and the modes of continuous sample introduction and collective sample introduction can be adopted during test. The device has simple structure and solves the problem of hard coupling of the capillary electrophoresis and the inductively coupled plasma mass spectrograph.
Description
Technical field
The present invention relates to chemical analysis field, more specifically relate to a kind of Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling interface device and using method thereof.
Background technology
Inductivity coupled plasma mass spectrometry ICP-MS is the new analysis and testing technology that grows up the eighties in 20th century.It combines and forms a kind of novel element and isotope analysis technology with the sensitive advantage of scanning fast of quadrupole rod mass spectrograph with the interfacing of uniqueness high temperature (7000K) ionization property with ICP-MS, can analyze most elements on the earth.With capillary electrophoresis technique and the existing report of analytical technique of mass spectrum coupling, still, still there is not commercial interface at present.The cruciform of having published and simple and easy shape capillary electrophoresis apparatus and inductively coupled plasma mass spectrometer coupling interface, can't solve that icp ms connects and dragging of causing and the peak broadening problem that produces and undue dilution make and sensitivity step-down problem can't provide very stable electrical connection simultaneously.
Summary of the invention
The objective of the invention is at the problems referred to above, a kind of Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling interface device are provided, this apparatus structure is simple, has solved the coupling difficult problem of Capillary Electrophoresis and icp ms.
It is characterized in that: comprise stainless steel capillary, sample solution peristaltic pump and T shape kapillary, described stainless steel capillary is connected with high-voltage power supply, its left end inlet is connected with miniflow sheath stream pipe with Capillary Electrophoresis respectively, and the output terminal of described stainless steel capillary is connected with T type kapillary input end through the sample solution peristaltic pump; Described T shape output terminal capillaceous inserts icp ms; T shape lower end capillaceous is connected with cleaning solution peristaltic pump output terminal.
Remarkable advantage of the present invention is:
1, solved the bottleneck difficult problem that Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling are used---stable electrical connection, overcome owing to icp ms connects dragging of causing and make the separating effect variation problem of Capillary Electrophoresis and undue dilution make sensitivity step-down problem.
2, have two kinds of quantitative models: continuous sample introduction pattern and collection sample introduction pattern.Continuous sample introduction pattern finding speed is fast, is suitable for the higher sample of concentration; It is highly sensitive to collect the sample introduction pattern, is fit to low concentration sample.Collecting under the sample introduction pattern, not only improved the separating effect of capillary electrophoresis apparatus, and improved the sensitivity that Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling are used greatly.Be used to connect Capillary Electrophoresis and icp ms, make icp ms (ICP-MS) can be used to measure element morphology, organometallics, and partial organic substances.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Wherein 1 for connecting cleaning solution, and 2 for connecting Capillary Electrophoresis, and 3 is current controller, and 4 is the cleaning solution peristaltic pump, 5 is the sample solution peristaltic pump, and 6 is stainless steel capillary, and 7 is T shape kapillary, 8 for connecting high-voltage power supply, and 9 for connecting icp ms, and 10 are miniflow sheath stream pipe.
Embodiment
The invention provides a kind of Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling interface device, embodiment comprises stainless steel capillary 6, sample solution peristaltic pump 5 and T shape kapillary 7, described stainless steel capillary is connected with high-voltage power supply, its left end inlet is connected with miniflow sheath stream pipe with Capillary Electrophoresis respectively, and the output terminal of described stainless steel capillary is connected with T type kapillary input end through the sample solution peristaltic pump; Described T shape output terminal capillaceous inserts icp ms; T shape lower end capillaceous is connected with cleaning solution peristaltic pump output terminal; This device is provided with respectively the current controller in order to control sample solution peristaltic pump and cleaning solution peristaltic pump, and described T shape kapillary is a polyethylene pipe.
Embodiment 1
When sample when capillary electrophoresis apparatus is separated, current controller control cleaning solution peristaltic pump 4 rotates, and stops sample solution peristaltic pump 5, can not influence the separation of capillary electrophoresis apparatus this moment; After a certain component separating in the sample is intact, current controller control cleaning solution peristaltic pump 4 stops, rotation sample solution peristaltic pump 5 is distributed into icp ms to one-tenth to be measured and measures, because peristaltic pump 4 has stopped, not auxiliary liquid is entered, so the sensitivity of icp ms is unaffected, different material concentration detection limit is as shown in table 1 under collection sample introduction pattern.
Table 1
| Determinand | Collect concentration detection limit (μ g/L) under the sample introduction |
| Trivalent arsenic, pentavalent arsenic, monomethyl arsenic, dimethyl arsenic, arsenic betaine, arsenocholine, arsanilic acid | 0.030～0.042 |
| Trimethyltin chloride, ethyl tin chloride, tripropyl tin chloride, tributyltin chloride | 0.090～0.190 |
Embodiment 2
When sample when capillary electrophoresis apparatus is separated, current controller control cleaning solution peristaltic pump 4 and sample solution peristaltic pump 5 rotate simultaneously with friction speed, being admitted to inductivity coupled plasma mass spectrometry immediately when a certain component separating in the sample flows out kapillary measures, finding speed is very quick and dead volume is little, thereby realizes continuous sample introduction.Different material concentration detection limit is as shown in table 2 under the continuous sample introduction pattern.
Table 2
| Determinand | Concentration detection limit under the continuous sample introduction (μ g/L) |
| Trivalent arsenic, pentavalent arsenic, monomethyl arsenic, dimethyl arsenic, arsenic betaine, arsenocholine, arsanilic acid | 0.1～0.2 |
| Trimethyltin chloride, ethyl tin chloride, tripropyl tin chloride, tributyltin chloride | 0.2～0.7 |
Embodiment 1 and 2 pump speed are such as following table 3:
Table 3
| Determinand | Concentration detection limit under the continuous sample introduction (μ g/L) | Collect concentration detection limit (μ g/L) under the sample introduction | Pump (5) speed (μ L/min) | Pump (4) speed (μ L/min) |
| Trivalent arsenic, pentavalent arsenic, monomethyl arsenic, dimethyl arsenic, arsenic betaine, arsenocholine, arsanilic acid | 0.1～0.2 | 0.030～0.042 | 12 | 120 |
| Trimethyltin chloride, ethyl tin chloride, tripropyl tin chloride, tributyltin chloride | 0.2～0.7 | 0.090～0.190 | 12 | 200 |
The speed basic fixed of pump 5 is 12 μ L/min, the speed of pump 4 is relevant with determinand character, fibrous root is specifically done a little adjustment according to determinand character, and generally between 100～200 μ L/min, the pump speed when inductivity coupled plasma mass spectrometry produces maximum signal to noise ratio is best pump speed.
Claims (6)
1. Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling interface device, it is characterized in that: comprise stainless steel capillary, sample solution peristaltic pump and T shape kapillary, described stainless steel capillary is connected with high-voltage power supply, its left end inlet is connected with miniflow sheath stream pipe with Capillary Electrophoresis respectively, and the output terminal of described stainless steel capillary is connected with T type kapillary input end through the sample solution peristaltic pump; Described T shape output terminal capillaceous inserts icp ms; T shape lower end capillaceous is connected with cleaning solution peristaltic pump output terminal.
2. Capillary Electrophoresis according to claim 1 and inductively coupled plasma mass spectrometer coupling interface device is characterized in that: described device is provided with respectively in order to control the current controller of sample solution peristaltic pump and cleaning solution peristaltic pump.
3. Capillary Electrophoresis according to claim 1 and 2 and inductively coupled plasma mass spectrometer coupling interface device is characterized in that: described T shape kapillary is a polyethylene pipe.
4. the using method that will remove 1 or 2 described Capillary Electrophoresis and inductively coupled plasma mass spectrometer coupling interface device as right is characterized in that: adopt continuous sample introduction to measure and collect sample introduction and measure.
5. the using method of Capillary Electrophoresis according to claim 4 and inductively coupled plasma mass spectrometer coupling interface device, it is characterized in that: described continuous sample introduction is determined as: when sample separates in capillary electrophoresis apparatus, current controller control cleaning solution peristaltic pump and sample solution peristaltic pump rotate simultaneously with friction speed, when a certain component separating in the sample flows out kapillary, be admitted to inductivity coupled plasma mass spectrometry immediately and measure, realize continuous sample introduction mensuration.
6. the using method of Capillary Electrophoresis according to claim 4 and inductively coupled plasma mass spectrometer coupling interface device, it is characterized in that: described collection sample introduction is determined as: when sample when capillary electrophoresis apparatus is separated, current controller control cleaning solution peristaltic pump rotates, and stops the sample solution wriggling; After a certain component separating in the sample was intact, current controller control cleaning solution peristaltic pump stopped, and rotation sample solution peristaltic pump is distributed into icp ms to one-tenth to be measured and measures.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018036160A (en) * | 2016-08-31 | 2018-03-08 | 三菱マテリアル株式会社 | Induction coupling plasma mass analysis method |
| CN108962716A (en) * | 2018-06-19 | 2018-12-07 | 中国科学院光电研究院 | A kind of detection device and its detection method of high-precision mass spectrum detection limit |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1499200A (en) * | 2002-11-08 | 2004-05-26 | 中国科学院大连化学物理研究所 | Microflow pump for combining use between capillary electrophoresis/electrochromatography and mass spectrum |
| CN2608988Y (en) * | 2003-04-17 | 2004-03-31 | 中国科学院长春应用化学研究所 | Twin T-type capillary electrophoresis-plasma mass spectrometer one-line interface |
| CN201034985Y (en) * | 2007-03-16 | 2008-03-12 | 福州大学 | No assistant flow high sensitive capillary electrophoresis and inductively coupled plasma mass spectrometer coupling interface |
| CN101042382A (en) * | 2007-03-20 | 2007-09-26 | 福州大学 | No assistant flow high sensitive capillary electrophoresis and inductively coupled plasma mass spectrometer coupling interface |
| CN201434855Y (en) * | 2009-06-16 | 2010-03-31 | 福州大学 | Linking interface device of capillary tube electrophoresis and jigger coupling plasma mass spectrograph |
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2009
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Non-Patent Citations (1)
| Title |
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| 康建珍等: "毛细管电泳-电感耦合等离子质谱联用的接口设计", 《分析化学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018036160A (en) * | 2016-08-31 | 2018-03-08 | 三菱マテリアル株式会社 | Induction coupling plasma mass analysis method |
| CN108962716A (en) * | 2018-06-19 | 2018-12-07 | 中国科学院光电研究院 | A kind of detection device and its detection method of high-precision mass spectrum detection limit |
| CN108962716B (en) * | 2018-06-19 | 2023-10-20 | 中国科学院光电研究院 | Detection device and detection method for mass spectrum detection limit |
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| CN101576532B (en) | 2013-03-20 |
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