CN101556274B - Human body vestige visualization reagent, preparation method and application thereof - Google Patents
Human body vestige visualization reagent, preparation method and application thereof Download PDFInfo
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- CN101556274B CN101556274B CN200910011169A CN200910011169A CN101556274B CN 101556274 B CN101556274 B CN 101556274B CN 200910011169 A CN200910011169 A CN 200910011169A CN 200910011169 A CN200910011169 A CN 200910011169A CN 101556274 B CN101556274 B CN 101556274B
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Abstract
The invention discloses a human body vestige visualization reagent, a preparation method and the application thereof. The human body vestige visualization reagent is suitable for discovering and visualizing various difficult vestiges and mainly comprises the following raw materials according to the parts by weight: 0.5-4 of biological indicator, 0.5-2 of phenol, 0.5-2 of choline Chloride, 1-5 of OP-10, 1-5 of TX-10, 1-5 of 40% of sodium hydroxide solution, 40-80 of sherwood oil solution, and 80-120 of absolute ethyl alcohol solution. The human body vestige visualization reagent is obtained by mixing the raw materials, confecting stock solution and preparing working solution. The invention has reasonable formulation, simple process, convenient operation, and is beneficial to be applied. The application proves that the invention is suitable for quickly seeking, discovering, visualizing vestiges such as sweat, occult blood, sweat ashes, blood ashes, occult blood, secretion and the like on various difficult bodies bearing marks, has the advantages of high sensitivity, high showing rate, good visualization effect, fast visualization speed, low cost and environment-friendly.
Description
Technical field
The present invention relates to a kind of vestige visualization reagent, especially relate to and a kind ofly be fit to that multiple difficult vestige is found and the human body vestige visualization reagent that manifests, belong to the police criminal detection field.
Background technology
The key that criminal case crime scene is reconnoitred be seek, find, manifest, fixed extraction offender is retained in on-the-spot potential trace evidence.The potential vestige that the scene of a crime is left over all is human body vestige (impression of the hand or a footprint); The formation medium majority of human body vestige is potential; As: (naked eyes are invisible for occult blood; But composition according to), sweat, seminal fluid, vaginal fluid, dust, tear, saliva, beverage, oil (animal oil, vegetable oil, mineral oil) etc.; What vestige was left in the murder scene holds trace body textile, leather, marble, brick and tile, paper, concrete floor, coating wall, various melon and fruit peel, beverage package etc. often, is divided into by its performance oozing diffusing property (like paper, textile etc.), non-ly ooze diffusing property (marble, ceramic tile etc.), partly oozing diffusing property (coating wall, leather etc.) and hold the trace body.The potential vestige that is retained in the murder scene belongs to difficult vestige, forms the medium composition of vestige and hold the trace body all to make and adopt the trace evidence obtaining and have very big difficulty.
The technical method of existing searching, discovery human body vestige is to rely on naked eyes on the printing opacity object, to be observed; Utilize reflected light on glossy object, to find; Fumigate with steam and to find potential vestige, these methods only are fit to the observation that minority is held potential vestige on the trace body, can not manifest, the fixed extraction vestige.
Manifesting of potential vestige is with vestige and hold the certain contrast of formation between the trace body, so that fixed extraction.Existing appearing technique can be divided into solid reagent appearance method, microparticle suspending liquid appearance method, liquid reagent appearance method, gaseous reagent appearance method.
Solid reagent has the powder appearance method; Be suitable for smooth, dry, impermeability and hold manifesting of fresh sweat latent fingerprint on the trace body (like glass, porcelain, paint wood etc.), the deficiency of existence is that to manifest the vestige kind single, and it is short to manifest timeliness; It is low to show rate, narrow application range.The microparticle suspending liquid method; Can dive to the sweat that is rich in lipid, the occult blood impression of the hand has certain effect that manifests, but sensitivity is not high, it is very low to show rate; It is narrow to manifest scope; Only non-ly ooze diffusing property and hold that the sweat of leaving on the trace surface is dived, the occult blood impression of the hand has certain produce effects, need after manifesting just can see impression of the hand clearly, destroy original trace evidence easily through flushing to minority is smooth.Complicated operation is not suitable for rig-site utilization.
The liquid reagent appearance method comprises ninhydrin method, and the manifesting of fresh or outmoded sweat fingerprint on the only suitable paper class object, it is very slow to show speed, and the scope of application is very narrow.Silver nitrate appearance method, itself and ninhydrin method are fit to the paper class equally and hold manifesting of fresh sweat latent fingerprint on the trace body, and sensitivity is lower, is difficult for preserving, and fixing means is complicated, is not suitable for rig-site utilization.DFO (1,8-carboline nine ketone) method, product has fluorescence, is fit to the fresh sweat latent fingerprint that the paper class holds on the trace body and manifests, and need under excitation source, observe, fix, extract, and is not suitable for on-the-spot difficult vestige and manifests and use.The physical developer method, the test solution preparation is complicated, operates very complicacy, and only effective to the occult blood vestige that the paper class is held on the trace body, it is very low to show rate, is unfavorable for that on-the-spot difficult vestige manifests.The tetramethyl benzidine method, only suitable paper class is held the occult blood impression of the hand on the trace body, and that reagent is prone to ooze is diffusing, trickling, the destructible vestige, sensitivity, to show rate not high, and the scope of application is narrow.Amino black 10B manifests the blood stain mark also has certain effect, but sensitivity is not high, and it is very low to show rate, and the suitable trace body scope of holding is narrow.Need after manifesting just can see impression of the hand clearly, destroy original trace evidence easily through flushing.Complicated operation is not suitable for on-the-spot difficult vestige and manifests.
The gaseous reagent appearance method has 502 " glue (ECA) appearance method, generate white polymerizate, show potential impression of the hand.Be fit to smooth, dark color and hold the sweat latent fingerprint of leaving on the trace body, light color hold on the trace body the sweat latent fingerprint through " 502 " glue is smoked show after, need dyeing to handle and just can show, it is slower to manifest speed, operation is complicacy, it is single to manifest the vestige kind.Iodine is smoked appearance method, is fit on the object such as paper fresh sweat and dives and oily latent fingerprint, and the scope of application is narrow, and it is very low to show rate, and complicated operation, iodine are prone to distillation, must be fixing rapidly after showing, and fixing means is complicated, is unfavorable for execute-in-place.This shows, existing vestige searching, discovery, process for show narrow application range, it is single to manifest the vestige kind, complicated operation, it is low to show rate, and it is short to manifest timeliness, manifests weak effect, and it is slow to manifest speed, and majority is only limited to laboratory operation, does not meet the needs of site inspection.
Summary of the invention
The present invention proposes to the problem that above-mentioned prior art exists; Purpose provides a kind of discovery that is fit to multiple difficult vestige, manifests; The human body vestige that the murder scene is left over adopts spray on a large scale apparent; Can find to rely on naked eyes and other conceptions of technology examine less than potential vestige; Have simple to operate, easy to carry, widely applicable, highly sensitive, show the rate height, manifest timeliness long, show fireballing characteristics, be a kind of human body vestige visualization reagent that is particularly suitable for the difficult vestige that criminal scene is left over is sought, found and manifests fast and preparation method thereof.
Another object of the present invention is the application during human body vestige visualization reagent is found, manifested at various vestiges.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is: human body vestige visualization reagent is characterized in that it mainly is to be formed fully by the following raw materials in parts by weight numeral system:
Biological indicator 0.5～4; Phenol 0.5～2; Choline Chloride 0.5～2; OP-101～5; TX-101～5; 40% sodium hydroxide solution 1～5; Petroleum ether solution 40～80; Ethanol solution 80～120.
Described human body vestige visualization reagent, it mainly is to be formed fully by the following raw materials in parts by weight numeral system: biological indicator 1.6～2.5; Phenol 0.5～1.5; Choline Chloride 1～2; OP-102～3; TX-102～4; 40% sodium hydroxide solution 3～4; Petroleum ether solution 60～70; Ethanol solution 100～120.
Described biological indicator is to be selected from by 1: 1: 1: 1: 1 methyl violet and bromothymol blue and alphanaphthol phthalein and thymolphthalein and phenolphthalein or by 2: 1: 1 basic fuchsin and cresol red with alphanaphthol phthalein or by 1: 2: 2: 2 peacock green and bromcresol green and bromcresol purple and cresol red or press 1: 1: 1 bromjophenol blue and cresol red and bromcresol purple or press 2: 1: 1 bromcresol green and in methyl red and the Bromothymol blue any one.
Described human body vestige visualization reagent also comprises triketohydrindene hydrate 0.5～3.
Described human body vestige visualization reagent also comprises xylenol orange 0.5～2.
Described human body vestige visualization reagent also comprises copper sulphate 0.5～1.
Described human body vestige visualization reagent also comprises soudan III 0.5～2.
Prepare the method for described human body vestige visualization reagent, comprise the following steps:
Raw material mixes:
Get each raw material by above-mentioned parts by weight, the raw material that biological indicator is required adds 40% sodium hydroxide solution earlier, grinds evenly back and the abundant mixing for standby use of other each raw material of being got with mortar;
Preparation stoste:
The raw material of above-mentioned mixing for standby use is added the ethanol solution stirring and dissolving by above-mentioned parts by weight, even with adding by the petroleum ether and stirring of above-mentioned parts by weight in this mixed liquor again;
Preparation work liquid:
The stoste of getting above-mentioned preparation is put into catalyst TX-10 and the catalyst OP-10 that blender adds by above-mentioned parts by weight and is stirred, and mixing time is 1h～2h, and it is fully mixed, and promptly is mixed with working fluid.
Above-mentioned each step is all carried out under normal temperature and normal pressure.
Application during said human body vestige visualization reagent is found, manifested at various vestiges.
Human body vestige of the present invention manifests principle and is that the main composition to sweat is an organic principle amino acid, and inorganic constituents acid radical anion and metal cation and most of water constitute, and the pH value of sweat is near neutrality or in little slant acidity scope.The principal ingredient of blood is made up of protein, fat and water, and the pH value of blood is in inclined to one side subacidity or near neutral range.Secretion mainly is to be made up of protein, the inclined to one side subacidity of pH value, and animal oil and vegetable oil mainly are to be made up of saturated fatty acid and unsaturated fatty acid in the oil, the inclined to one side subacidity of its pH value or near neutral range.Contain molysite, silicate, carbonate, corruption in the composition of dust and plant matter, bacterium etc.; PH value is in alkalescence or neutral range, and human body vestige visualization reagent of the present invention is with biological indicator, PH indicator, helps toner, lysochrome, metal cation intercalating agent, catalyst, mordant, protein-complexing agents etc. organically to combine, under the cushioning liquid condition; It plays complementation each other; The detection principle and the means of applied analysis chemistry are carried out qualitative chromogenic assay to human body vestige, and the multiple composition of vestige medium is manifested simultaneously; Increased the sensitivity of visualization reagent and manifested timeliness, strengthened and manifest effect and show rate.Widened and manifested scope, played the effect of the various process for show advantages of integrated application.
The effect of the biological indicator that is adopted among the present invention is by physical factor and chemical factor and carry out.Physical factor such as cell and cellular material are to capillarity, infiltration, the suction-operated of dyestuff.Chemical factor then is the various chemical reactions that the heterogeneity according to cellular material and dyestuff takes place.Acidic materials are prone to absorption for basic-dyeable fibre, and suction-operated is firm; Equally, alkaline matter is easy to absorption to acid dyes.Methyl violet, peacock green, basic fuchsin, methyl red, bromcresol green, Bromothymol blue, bromjophenol blue, bromcresol purple, cresol red, bromothymol blue, alphanaphthol phthalein, thymolphthalein, phenolphthalein be one type in its specific pH value scope; Change and the biological indicator of variable color with the solution pH value, they are organic monoacid or weak base.When the solution pH value changes, when losing proton, biological indicator becomes the alkaline color composition by sour colour content, and obtain proton and become sour colour content by the alkaline color composition; In transition process, because the change of this body structure of biological indicator, thereby cause the variation of solution colour.The pH value of sweat, blood, other secretion of human body, dust is all near neutral; Can make the structural change of this type biological indicator be certain color; Form contrast and make vestige present certain color and hold the trace body; Particularly adopt the mixing of different reagent in the biological indicator, can shorten transition interval and the sharp intelligence that improves variable color, widen color change interval.
The triketohydrindene hydrate that is adopted among the present invention is amino acid whose sensitive developer, and itself and amino acid generation color reaction generate the bluish violet complex compound, and the sensitivity of human body vestige visualization reagent is strengthened, and contrast is more obvious, works the look effect that helps.
Phenol that is adopted among the present invention and Choline Chloride; It can make protein denaturation, and metaprotein easy and separate nucleic acid and deposition increase the indicator coloration; Increase the polarity of protein; The dyeing group of indicator is adsorbed on the protein more quickly, and the coloring agent difficult drop-off plays mordant.
The compound of the APES that is adopted among the present invention: TX-10, APES: Op-10 is emulsifying agent, spreading agent, cleanser, dye leveller in chemical industry or the chemical experiment etc., has premium properties such as emulsification, level dyeing, moistening and diffusion.Human body vestige visualization reagent becomes droplet after emulsification, play dispersion effect.Experiment shows, TX-10, when Op-10 exists, and the ionic dissociation equilibrium of biological indicator and color change interval thereof will change, and select these two kinds of micellar solutions as catalyst, reach the range of application that enlarges biological indicator, also reach the firmness that improves dyeing simultaneously.
The xylenol orange that is adopted among the present invention is a purple crystal, and is soluble in water.It is o'clock apparent redness in pH＞6.3, color in the middle of being during pH=6.3.The complex compound of xylenol orange and metallic ion is purpureal, with xylenol orange as indicator, to Bi
3+, Zn
2+, Pb
2+, Hg
2+Plasma dyeing can get very well results, and these heavy metal cations are prone to precipitate under alkali condition with protein, help reagent dyeing.
The soudan III that is adopted among the present invention as lysochrome, it is dissolved in the ethanol, the soudan III dye liquor, this dye liquor can dye fat, can also dye cork, cuticula, can dye to the grease in sweat mixed with dirt and the grease vestige medium.
The copper sulphate that is adopted among the present invention as protein-complexing agents, it can make protein precipitation, and when pH value of solution value during greater than isoelectric point, protein particulate is electronegative, so just easily and heavy metal ion (Mg
2+, Pb
2+, Cu
2+, Ag
+Deng) form insoluble salt and precipitate; Through the biuret reaction of copper sulphate and protein, contain many in the protein molecule and the peptide bond biuret structural similarity, therefore also can play biuret reaction; Form violet complex, come qualitative identification of protein effect remarkable with this reaction.
The absolute ethyl alcohol that is adopted among the present invention, sherwood oil belong to nontoxic or low toxicity, environment-friendly type solvent.Absolute ethyl alcohol has the ankyrin effect, dissolving each other between two kinds of solvents, mixed solvent to the solute effect of solute greater than single solvent.
The present invention's advantage and beneficial effect compared with prior art is following:
The present invention fills a prescription rationally, and technology is simple, easy to operate, is beneficial to application, through using proof; Widely applicable, all can seek fast, find, manifest for sweat, occult blood, vestiges such as sweat is grey, blood is grey, secretion that multiple difficulty is held on the trace body, be highly sensitive, show the rate height; Good developing effect, it is fast to manifest speed, and it is long to manifest timeliness; And cost is low, and is simple to operate, the visualization reagent of environment-friendly type.The present invention integrates the advantage of existing appearing technique, and the human body vestige that the murder scene is left over all has extraordinary produce effects, has filled up the blank that existing integrated application is sought, found, manifests difficult vestige technical method.
Description of drawings
Fig. 1 is the photo that the present invention manifests sweat ash impression of the hand on the lamppost.
Fig. 2 is the photo that the present invention manifests the blood ash impression of the hand on the rubber.
Fig. 3 is the photo that the present invention manifests blood ash footprint on the concrete floor.
Fig. 4 is the photo that the present invention manifests the dust hand print on the rustproof lacquer wall.
Fig. 5 is the photo that the present invention manifests the occult blood impression of the hand of leaving over 20 days on the aluminium alloy.
Fig. 6 is that the present invention manifests the photo of leaving over 4 days secretion impression of the hand on the pvc pipe.
Fig. 7 is the photo that the present invention manifests the blood and sweat impression of the hand of leaving over 4 hours on the marble countertop.
Embodiment
Referring to Fig. 1 to Fig. 7 of the present invention and combine specific embodiment that the present invention is further elaborated, but protection scope of the present invention is not limited by concrete embodiment, is as the criterion with claims.In addition, with under the prerequisite of technical scheme of the present invention, any change or change that those of ordinary skills that the present invention did are realized easily all will fall within the claim scope of the present invention.
Embodiment 1
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Methyl violet 0.1g; Bromothymol blue 0.1g; Alphanaphthol phthalein 0.1g; Thymolphthalein 0.1g; Phenolphthalein 0.1g; Phenol 2g; Choline Chloride 2g; OP-105g; TX-105g; 40% sodium hydroxide solution 5g; Petroleum ether solution 80g; Ethanol solution 120g.
Preparation method of the present invention is following:
Raw material mixes: methyl violet, bromothymol blue, alphanaphthol phthalein, thymolphthalein, the phenolphthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride of even back and above-mentioned weight with mortar;
Preparation stoste: the raw material of above-mentioned mixing for standby use is added the ethanol solution stirring and dissolving of above-mentioned weight, again that the petroleum ether and stirring that adds above-mentioned weight in this mixed liquor is even;
Preparation work liquid: the stoste of getting above-mentioned preparation is put into catalyst TX-10, the catalyst OP-10 that blender adds above-mentioned weight and is stirred, and mixing time is 1h～2h, and it is fully mixed, and promptly is mixed with working fluid.
Above-mentioned each step is all carried out under normal temperature and normal pressure.
Embodiment 2
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Basic fuchsin 2g; Cresol red 1g; Alphanaphthol phthalein 1g; Phenol 0.5g; Choline Chloride 0.5g; OP-101g; TX-101g; 40% sodium hydroxide solution 1g; Petroleum ether solution 40g; Ethanol solution 80g.
Preparation method of the present invention is following:
Raw material mixes: basic fuchsin, cresol red, the alphanaphthol phthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride of even back and above-mentioned weight with mortar;
Other step is with embodiment 1.
Embodiment 3
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Peacock green 0.5g; Bromcresol green 1g; Bromcresol purple 1g; Cresol red 1g; Phenol 1.5g; Choline Chloride 1g; OP-103g; TX-103g; 40% sodium hydroxide solution 3g; Petroleum ether solution 60g; Ethanol solution 100g.
Preparation method of the present invention is following:
Raw material mixes: peacock green, bromcresol green, bromcresol purple, the cresol red of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 4
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromjophenol blue 0.8g; Cresol red 0.8g; Bromcresol purple 0.8g; Phenol 1g; Choline Chloride 1.5g; OP-102g; TX-104g; Triketohydrindene hydrate 3; Xylenol orange 0.5g; Copper sulphate 0.5g; Soudan III 0.5g; 40% sodium hydroxide solution 4g; Petroleum ether solution 70g; Ethanol solution 110g.
Preparation method of the present invention is following:
Raw material mixes: bromjophenol blue, cresol red, the bromcresol purple of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate, xylenol orange, copper sulphate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 5
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromcresol green 1.5g; Methyl red 0.75g; Bromothymol blue 0.75g; Phenol 1.2g; Choline Chloride 1.8g; OP-104g; TX-102.5g; Triketohydrindene hydrate 0.5g; Xylenol orange 2g; Copper sulphate 1g; Soudan III 2g; 40% sodium hydroxide solution 3.5g; Petroleum ether solution 50g; Ethanol solution 90g.
Preparation method of the present invention is following:
Raw material mixes: bromcresol green, methyl red, the Bromothymol blue of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate, xylenol orange, copper sulphate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 6
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Methyl violet 0.6g; Bromothymol blue 0.6g; Alphanaphthol phthalein 0.6g; Thymolphthalein 0.6g; Phenolphthalein 0.6g; Phenol 1.7g; Choline Chloride 0.8g; OP-104.5g; TX-101.5g; Triketohydrindene hydrate 1.7g; Copper sulphate 0.8g; 40% sodium hydroxide solution 2.8g; Petroleum ether solution 65g; Ethanol solution 105g.
Preparation method of the present invention is following:
Raw material mixes: methyl violet, bromothymol blue, alphanaphthol phthalein, thymolphthalein, the phenolphthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate, copper sulphate of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 7
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Basic fuchsin 1g; Cresol red 0.5g; Alphanaphthol phthalein 0.5g; Phenol 0.6g; Choline Chloride 0.95g; OP-102.5g; TX-102g; Xylenol orange 1.2g; Soudan III 1.3g; 40% sodium hydroxide solution 4.5g; Petroleum ether solution 55g; Ethanol solution 85g.
Raw material mixes: basic fuchsin, cresol red, the alphanaphthol phthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, xylenol orange, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 8
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Peacock green 0.3g; Bromcresol green 0.6g; Bromcresol purple 0.6g; Cresol red 0.6g; Phenol 1.8g; Choline Chloride 0.7g; OP-101.5g; TX-103.5g; Soudan III 0.7g; 40% sodium hydroxide solution 5g; Petroleum ether solution 45g; Ethanol solution 115g.
Preparation method of the present invention is following:
Raw material mixes: peacock green, bromcresol green, bromcresol purple, the cresol red of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 9
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromjophenol blue 1.2g; Cresol red 1.2g; Bromcresol purple 1.2g; Phenol 2g; Choline Chloride 0.5g; OP-103.5g; TX-101.5g; Triketohydrindene hydrate 2g; 40% sodium hydroxide solution 1.7g; Petroleum ether solution 75g; Ethanol solution 100g.
Preparation method of the present invention is following:
Raw material mixes: bromjophenol blue, cresol red, the bromcresol purple of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 10
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromcresol green 1.6g; Methyl red 0.8g; Bromothymol blue 0.8g; Phenol 0.5g; Choline Chloride 2g; OP-103.5g; TX-104g; Triketohydrindene hydrate 3g; Soudan III 0.5g; 40% sodium hydroxide solution 3g; Petroleum ether solution 70g; Ethanol solution 80g.
Preparation method of the present invention is following:
Raw material mixes: bromcresol green, methyl red, the Bromothymol blue of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 11
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Basic fuchsin 1.4g; Cresol red 0.7g; Alphanaphthol phthalein 0.7g; Phenol 1.3g; Choline Chloride 2g; OP-105g; TX-102g; Xylenol orange 1.5g; Copper sulphate 0.6g; 40% sodium hydroxide solution 4.2g; Petroleum ether solution 80g; Ethanol solution 95g.
Preparation method of the present invention is following:
Raw material mixes: basic fuchsin, cresol red, the alphanaphthol phthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, xylenol orange, copper sulphate of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 12
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromjophenol blue 0.55g; Cresol red 0.55g; Bromcresol purple 0.55g; Phenol 0.9g; Choline Chloride 1.6g; OP-103.8g; TX-104.5g; Copper sulphate 0.5g; Soudan III 2g; 40% sodium hydroxide solution 2.5g; Petroleum ether solution 40g; Ethanol solution 120g.
Preparation method of the present invention is following:
Raw material mixes: bromjophenol blue, cresol red, the bromcresol purple of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, copper sulphate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Embodiment 13
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Bromcresol green 1.8g; Methyl red 0.9g; Bromothymol blue 0.9g; Phenol 2g; Choline Chloride 1g; OP-103g; TX-102g; Xylenol orange 1.2g; 40% sodium hydroxide solution 5g; Petroleum ether solution 60g; Ethanol solution 110g.
Preparation method of the present invention is following:
Raw material mixes: bromcresol green, methyl red, the Bromothymol blue of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, xylenol orange of even back and above-mentioned weight with mortar; Other step is with embodiment 1
Embodiment 14
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Methyl violet 0.8g; Bromothymol blue 0.8g; Alphanaphthol phthalein 0.8g; Thymolphthalein 0.8g; Phenolphthalein 0.8g; Phenol 0.5g; Choline Chloride 1g; OP-102.5g; TX-103g; Triketohydrindene hydrate 2g; Xylenol orange 0.5g; Copper sulphate 1g; Soudan III 1.1g; 40% sodium hydroxide solution 1.5g; Petroleum ether solution 50g; Ethanol solution 100g.
Preparation method of the present invention is following:
Raw material mixes: methyl violet, bromothymol blue, alphanaphthol phthalein, thymolphthalein, the phenolphthalein of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, triketohydrindene hydrate, xylenol orange, copper sulphate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1
Embodiment 15
The present invention is formed by the following raw materials in parts by weight numeral system fully:
Peacock green 0.4g; Bromcresol green 0.8g; Bromcresol purple 0.8g; Cresol red 0.8g; Phenol 1.4g; Choline Chloride 2g; OP-101g; TX-105g; Xylenol orange 2g; Copper sulphate 0.8g; Soudan III 1.5g; 40% sodium hydroxide solution 2g; Petroleum ether solution 75g; Ethanol solution 105g.
Preparation method of the present invention is following:
Raw material mixes: peacock green, bromcresol green, bromcresol purple, the cresol red of getting above-mentioned weight add 40% sodium hydroxide solution, grind the abundant mixing for standby use of phenol, Choline Chloride, xylenol orange, copper sulphate, soudan III of even back and above-mentioned weight with mortar; Other step is with embodiment 1.
Application implementation example 1
Adopt the formulated of the embodiment of the invention 1 to become working fluid, and under normal temperature and normal pressure, sweat ash vestige manifested as follows:
Place: sweat ash impression of the hand on the outdoor lamppost.
Method of operating:
Drip to show: draw the working fluid for preparing with the reagent dropper, holding the working fluid of the present invention on the trace position that stays of trace body, then with tap water wash show to vestige till.
Spray and show: in sprayer, load onto the working fluid for preparing, the sprayer aligning is held trace body vestige leave over the position spraying, require droplet fine and smooth, even; 45 centimetres of spraying distances; During spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 1 presents bluish violet.
Application implementation example 2
Adopt the formulated of the embodiment of the invention 2 to become working fluid, and under normal temperature and normal pressure, potential blood, blood ash vestige manifested as follows:
Place: the blood ash impression of the hand on the indoor rubber skin.
Method of operating:
Drip to show: the working fluid for preparing with the absorption of reagent dropper, drip reagent holding staying on the trace position of trace body, then with tap water wash show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 2 presents redness.
Application implementation example 3
Adopt the formulated of the embodiment of the invention 4 to become working fluid, and under normal temperature and normal pressure, potential blood, blood ash vestige manifested as follows:
Place: blood ash footprint on the indoor concrete floor.
Method of operating:
Drip to show: the working fluid for preparing with the absorption of reagent dropper, drip reagent holding staying on the trace position of trace body, then with tap water wash show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 3 presents purple.
Application implementation example 4
Adopt the formulated of the embodiment of the invention 5 to become working fluid, and under normal temperature and normal pressure, potential blood, blood ash vestige manifested as follows:
Place: the dust hand print on the outdoor coating wall.
Method of operating:
Drip to show: the working fluid for preparing with the absorption of reagent dropper, drip reagent holding staying on the trace position of trace body, then with tap water wash show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 4 presents bottle green.
Application implementation example 5
Adopt the formulated of the embodiment of the invention 3 to become working fluid, and under normal temperature and normal pressure, the occult blood vestige manifested as follows:
Place: leave over 20 days occult blood impression of the hand on the indoor aluminium alloy.
Method of operating:
Drip to show: the working fluid for preparing with the absorption of reagent dropper, drip reagent holding staying on the trace position of trace body, then with tap water wash show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 5 presents green or yellow green.
Application implementation example 6
Adopt the formulated of the embodiment of the invention 9 to become working fluid, and under normal temperature and normal pressure, the secretion vestige manifested as follows:
Place: leave over 4 days secretion impression of the hand on the indoor pvc pipe
Method of operating:
Drip to show: draw the working fluid for preparing with the reagent dropper, hold the trace body stay on the trace position dropping reagent to wash with tap water then to show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 6 presents purple.
Application implementation example 7
Adopt the formulated of the embodiment of the invention 14 to become working fluid, and under normal temperature and normal pressure, the blood and sweat vestige manifested as follows:
Place: leave over four hours blood and sweat impression of the hand on the outdoor marble countertop
Method of operating:
Drip to show: the working fluid for preparing with the absorption of reagent dropper, drip reagent holding staying on the trace position of trace body, then with tap water wash show to vestige till.
Spray shows: aim at sprayer and hold trace body vestige and leave over the position spraying, require droplet fine and smooth, evenly, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aim at and hold trace body vestige place, spray and can show for twice.
Manifest the result: vestige color as shown in Figure 7 presents purple.
Following table 1 is that human body vestige visualization reagent of the present invention and existing vestige appearing technique hold the comparison that various potential vestiges show rate on the trace body to 30 kinds:
Place: indoor and outdoor simulated field
Experiment sample is left over:
Leave over method by blood, grease, dust, secretion, sweat, beverage, blood ash, the grey trace sample of sweat, the imitation scene is left over situation and is left over.
Hold the trace body:
Ceramic tile, glass, plastics, leather, aluminium alloy, plastic-steel, coat of paint metal, cardboard, polyfoam, magazine paper, trade mark, adhesive tape, marble, concrete floor, coating wall, plated metal, pvc pipe, rubber, Renminbi, computer material, leaves of plants, melon and fruit peel, flooring laminate, paint wood, true qualities are wooden, totally 30 kinds on the metal that sprays paint, textile, brick, stone, leaf.
Method of operating:
Drip to show: the reagent that triketohydrindene hydrate, silver nitrate, DFO, amino black 10B, tetramethyl benzidine prepare with the absorption of reagent dropper drips reagent holding staying on the trace position of trace body, till showing to vestige.
Spray shows: human body vestige visualization reagent of the present invention manifests with the spray appearing method, and sprayer is aimed at and held trace body vestige and leave over the position spraying, 45 centimetres of spraying distances, during spraying with hold trace body angle 30 degree, aligning holds trace body vestige place, sprays and can show for twice.
Smoked showing: " 502 " glue (ECA) adopts fumigating and appearing method to manifest, and will wait to show to hold the trace body and place in the fumigation dispaly cabinet, smoke apparent about 30 minutes by automatic transmission.
The comparison that table 1 human body vestige visualization reagent and existing vestige appearing technique show rate to various potential vestiges
Annotate: show the ratio that vestige that rate representes that various process for show form on above-mentioned 30 kinds of different objects same media shows successfully sample and total sample number.100% representative is all showed the vestige that this medium forms; 0 representative can not be showed the vestige that this medium forms.
Obtaining human body vestige visualization reagent of the present invention and existing vestige appearing technique from above-mentioned contrast experiment, to compare the visualization reagent consumption few, shows the rate height, and it is fast to manifest speed; Sensitivity, good developing effect, it is long to manifest timeliness; Be not easy to ooze diffusing, vestige manifests clear, and vestige is left over the time; Temperature, humidity, objective environment, the dilutability of blood does not all have influence to the effect that manifests of human body vestige visualization reagent of the present invention.
Claims (8)
1. human body vestige visualization reagent is characterized in that it mainly is to be formed fully by the following raw materials in parts by weight numeral system:
Biological indicator 0.5～4; Phenol 0.5～2; Choline Chloride 0.5～2; OP-101～5; TX-101～5; 40% sodium hydroxide solution 1～5; Petroleum ether solution 40～80; Ethanol solution 80～120; Described biological indicator is to be selected from by 1: 1: 1: 1: 1 methyl violet and bromothymol blue and alphanaphthol phthalein and thymolphthalein and phenolphthalein or by 2: 1: 1 basic fuchsin and cresol red with alphanaphthol phthalein or by 1: 2: 2: 2 peacock green and bromcresol green and bromcresol purple and cresol red or press 1: 1: 1 bromjophenol blue and cresol red and bromcresol purple or press 2: 1: 1 bromcresol green and in methyl red and the Bromothymol blue any one.
2. according to claim 1 human body vestige visualization reagent, it is characterized in that it mainly is to be formed fully by the following raw materials in parts by weight numeral system: biological indicator 1.6～2.5; Phenol 0.5～1.5; Choline Chloride 1～2; OP-102～3; TX-102～4; 40% sodium hydroxide solution 3～4; Petroleum ether solution 60～70; Ethanol solution 100～120.
3. according to claim 1 or 2 human body vestige visualization reagents, it is characterized in that also comprising triketohydrindene hydrate 0.5～3.
4. according to claim 1 or 2 human body vestige visualization reagents, it is characterized in that also comprising xylenol orange 0.5～2.
5. according to claim 1 or 2 human body vestige visualization reagents, it is characterized in that also comprising copper sulphate 0.5～1.
6. according to claim 1 or 2 human body vestige visualization reagents, it is characterized in that also comprising soudan III 0.5～2.
7. prepare the method for claim 1 or 2 described human body vestige visualization reagents, comprise the following steps:
Raw material mixes:
Get each raw material by above-mentioned parts by weight, the raw material that biological indicator is required adds 40% sodium hydroxide solution earlier, grinds evenly back and the abundant mixing for standby use of other each raw material of being got with mortar;
Preparation stoste:
The raw material of above-mentioned mixing for standby use is added the ethanol solution stirring and dissolving by above-mentioned parts by weight, even with adding by the petroleum ether and stirring of above-mentioned parts by weight in this mixed liquor again;
Preparation work liquid:
The stoste of getting above-mentioned preparation is put into catalyst TX-10 and the catalyst OP-10 that blender adds by above-mentioned parts by weight and is stirred, and mixing time is 1h～2h, and it is fully mixed, and promptly is mixed with working fluid;
Above-mentioned each step is all carried out under normal temperature and normal pressure.
8. the application in finding, manifest at various human body vestiges according to the said human body vestige visualization reagent of claim 1 to 7.
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| CN200910011169A CN101556274B (en) | 2009-04-16 | 2009-04-16 | Human body vestige visualization reagent, preparation method and application thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1088076A (en) * | 1992-12-18 | 1994-06-22 | 丛吉升 | Display method of finger prints on rough object |
| CN101216428A (en) * | 2008-01-18 | 2008-07-09 | 中国人民公安大学 | Method for presenting latent fingermark adopting quantum point type photoluminescent reagent |
| CN101381368A (en) * | 2008-10-22 | 2009-03-11 | 江苏警官学院 | Ethylenediamine schiff base type sweat fingerprint fluorescent reagent, synthesis and use thereof |
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2009
- 2009-04-16 CN CN200910011169A patent/CN101556274B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1088076A (en) * | 1992-12-18 | 1994-06-22 | 丛吉升 | Display method of finger prints on rough object |
| CN101216428A (en) * | 2008-01-18 | 2008-07-09 | 中国人民公安大学 | Method for presenting latent fingermark adopting quantum point type photoluminescent reagent |
| CN101381368A (en) * | 2008-10-22 | 2009-03-11 | 江苏警官学院 | Ethylenediamine schiff base type sweat fingerprint fluorescent reagent, synthesis and use thereof |
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