CN101551391A - Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof - Google Patents
Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof Download PDFInfo
- Publication number
- CN101551391A CN101551391A CNA2009100274585A CN200910027458A CN101551391A CN 101551391 A CN101551391 A CN 101551391A CN A2009100274585 A CNA2009100274585 A CN A2009100274585A CN 200910027458 A CN200910027458 A CN 200910027458A CN 101551391 A CN101551391 A CN 101551391A
- Authority
- CN
- China
- Prior art keywords
- chloromycetin
- magnetic
- cap
- magnetic bead
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and a preparation method thereof, relating to the technical field of chloromycetin detection. The test strip consists of a lining board as well as a sample pad, a magnetic scale combination pad, a coated film and an absorbing pad which are sequentially engaged on the lining board; the magnetic scale combination pad is glass fiber for absorbing chloromycetin magnetic scale antibody; and the coated film is provided with an adelomorphic detection line T line which is printed by chloromycetin-coupled carrier protein solution, and an adelomorphic control line C line which is printed by goat anti-rabbit IgG solution. The sample pad of the test strip is inserted into the solution of a tested sample, then taken out after 10-20 seconds, and then reacts for 15 minutes at room temperature; then the test strip is arranged in a magnetic signal detector for detection, and the detector further outputs the biological responding signals which have been converted into magnetic field signals in the form of electrical signals; and after a specification curve is drawn, the chloromycetin content in the tested sample is calculated according to the specification curve. The invention relates to the method based on nano-magnetic immunological technique for detecting chloromycetin, and has the characteristics of high sensitivity, short reaction time, cheap instrument and equipment, convenience, easy usage and the like.
Description
Technical field
Immuomagnetic bead chromatographic test strip of a kind of fast detecting chloromycetin and preparation method thereof relates to chloromycetin detection technique field.
Background technology
Chloromycetin (Chloramphenicol, be called for short CAP) be the microbiotic of first complete chemical synthetic, because its inexpensive and good antibiotic property, the stable property of medicine, once in a period of time as the medicine for treatment of feed addictive and bacteriosis, usable range is wider.Chloromycetin is a kind of broad-spectrum antibiotic, and heavy dose use repeatedly can cause CAP residual in milk and dairy produce etc.Chloromycetin has certain detrimental effect of accumulating to stomach, intestines, liver etc., also can cause allergic reaction, suprainfection, teratogenesis tire, and easily cause the human body blood poisoning, causes irreversible alpastic anemia.Therefore various countries have all formulated strict limitation standard to chloramphenicol residue in the food.The residual chloromycetin problem has caused international organization and the great attention of many countries and regions in the world.European Union, the U.S. etc. stipulate in rules that all the residual chloromycetin limit standard is " zero tolerance ", promptly must not detect.The Chinese government provides against to herd in fishing and uses chloromycetin in the breed, and has strengthened the inspecting force to residual chloromycetin in the animal derived food.The detection method of the chloromycetin medicament residue of China's employing at present has microbial method, capillary zone electrophoresis method, high performance liquid chromatography, gas chromatography-mass spectrography, liquid chromatography-mass spectrography etc., but above-mentioned analytical approach all has following shortcoming: a succession of physics or chemical operation can be destroyed sample; Sample must dilute, filter, extract through multistep, and preparation is complicated, loaded down with trivial details.Gas chromatography mass spectrometry or LC-MS are the conclusive evidence methods of chloromycetin, but because its complex operation, and long sample pre-treatment and reduction process, cause detecting the cost height, cycle is long, can't satisfy the requirement of sample rapid screening in enormous quantities, is restricted and it is extensive use of.Immuno analytical method has higher sensitivity and specificity, and the purity requirement to sample during detection is not high and easy and simple to handle, is applicable to the detection of great amount of samples.Enzyme linked immunosorbent assay (Enzyme-linked Immuno-sorbent Assay, abbreviate ELISA as) and colloidal gold immunity chromatography (Colloidal Gold Irnmuno-Chromatography Assay, abbreviate GICA as) since its cheapness, characteristics such as special, sensitive and quick be widely used in the rapid screening of residue of veterinary drug.But ELISA reagent needs special-purpose laboratory and professional to test, complicated operation, and the time is longer; Collaurum class reagent is easy and simple to handle, quick, the scene that is suitable for is detected, but sensitivity is low slightly, and the result is Direct observation with the naked eye, can produce error, also can not record.
The nano immune magnetic bead is the super paramagnetic nano particle that contains ferro element, and the outside is coating the polyethylene kind polymer substance, and can have carboxyl or amino, is used for and cross linking of protein molecule.Immunomagnetic beads all is applied in cell and big molecular separation, purifying and diagnosis.
It is as follows that the nano immune magnetic bead detects the test paper ultimate principle: adopt competition law, i.e. chloromycetin CAP in the sample and the chloromycetin polyclonal antibody that is fixed on the envelope antigen CAP-OVA competition marked by magnetic bead on the coated film.
Utilize a kind of antibody of immunomagnetic beads mark, bag is anti-by corresponding envelope antigen and goat-anti rabbit two on the NC of test paper film.After test strips is with the terminal immersion of sample pad sample, sample solution passes through capillarity swimming from the bottom up along test strips, dissolving magnetic mark pad is gone up dry magnetic labeling antibody, if do not have medicine to be measured in the testing sample, then immune response can direct swimming take place to the coating antigen CAP-OVA on detection line (T line) and the nitrocellulose membrane in the magnetic labeling antibody, thereby magnetic bead particles is assembled, form the lines of black, other unconjugated magnetic labeling antibody continues by capillarity swimming forward then, with the goat-anti rabbit two anti-generation immune responses for the second time on the control line (C line), the same black lines that forms, just have two black lines like this on the coated film, the expression sample is negative.If have medicine to be measured in the testing sample, then the magnetic labeling antibody at first can with the detection thing generation immune response in the sample, when the magnetic labeling antibody that does not react has residue, can with CAP-OVA immune response take place just on detection line, form black lines, the lines intensity when its color intensity is weaker than feminine gender; And when the magnetic labeling antibody all with sample in medicine chloromycetin CAP to be measured take place immunity in conjunction with the time, just do not have again antibody to combine, thereby detection line does not just have the black lines appearance with the detection line coating antigen.Whether control line is effectively set for check magnetic mark immune chromatography method itself, so no matter whether have medicine chloromycetin CAP to be measured in the sample, control line all should manifest.If control line does not develop the color, illustrate that then test strips lost efficacy.Realize result's judgement by measuring the content of catching magnetic bead on the detection line at last.Compare with the colloid gold test paper class, the difference that immunomagnetic beads detects the test paper maximum is to change the mark collaurum into the mark magnetic bead, with Magnetic Assay Reader the magnetic bead content on the detection line is detected during testing result and magnetic signal is changed into electric signal, rather than rely on naked eyes to go total judgement, can make the result judge more accurate, sensitive, objective like this and be convenient to recorded and stored.Utilize nano immune magnetic bead development chloramphenicol antibody to detect fast diagnose test paper and still do not have report.
Summary of the invention
Purpose of the present invention: at the deficiency and the defective of the technology that has chlorine detection mycin CAP now, but a kind of detection method based on fast detecting chloromycetin nanometer magnetic immunological technique, highly sensitive, that the reaction time is short, instrument and equipment is cheap is provided, makes it can detect the residue of veterinary drug of chloromycetin in the animal quick more, sensitive, easily.
Technical scheme of the present invention: a kind of chloromycetin immuomagnetic bead chromatographic test strip, form by liner plate and the sample pad that on liner plate, is connected successively, magnetic mark pad, coated film, adsorptive pads, magnetic mark pad is the glass fibre cotton of absorption chloromycetin magnetic labeling antibody, the stealthy detection line T of the orthoscopic that the carrier protein solution of useful chloromycetin coupling on coated film is printed line is with the stealthy control line C of the orthoscopic line of goat anti-rabbit igg solution printing;
Hard plastic bar or the cardboard bar of described liner plate for not absorbing water; Sample pad is glass fibre cotton, nylon membrane, PVDF membrane or polyester film; Coated film is nitrocellulose filter, pure cellulose film or carboxylation cellulose membrane; Adsorptive pads is absorbent filter or filter paper for oil;
Described chloromycetin magnetic labeling antibody is the polyclonal antibody of the chloromycetin of marked by magnetic bead;
Described magnetic bead is the Fe that finishing has carboxyl
3O
4Magnetic bead;
The carrier protein of described coupling chloromycetin is: bovine serum albumin(BSA) BSA, the pure albumen OVA of ovum gallinaceum.
The preparation method of described chloromycetin immuomagnetic bead chromatographic test strip:
1) the synthetic and evaluation of chloromycetin complete antigen: adopt mixed anhydride method respectively with BSA in DMF solution, OVA and chloromycetin CAP carry out coupling, obtain the holoantigen CAP-BSA and the CAP-OVA of chloromycetin, and utilize ultraviolet scanner to identify;
2) Polyclonal Antibody Preparation and purifying: as immunogene, immune new zealand rabbit prepares the CAP polyclonal antibody and according to a conventional method with the ammonium sulfate purifying with CAP-BSA;
3) preparation of immunomagnetic beads: with the nanometer magnetic bead coupling of CAP antibody with the band carboxyl, preparation contains the immunomagnetic beads of CAP antibody, i.e. chloromycetin magnetic labeling antibody; Prepare magnetic mark pad with glass fibre cotton absorption chloromycetin magnetic labeling antibody;
4) embedding CAP-OVA and goat anti-rabbit igg are to coated film: the coating antigen CAP-OVA spray that will select concentration respectively with spray film instrument is stated from the T line of coated film, and the goat anti-rabbit igg spray is stated from the C line of coated film, and 37 ℃ of oven drying 10min are standby;
5) making of test strip: coated film, the adsorptive pads of sample pad, magnetic being marked pad, embedding coating antigen CAP-OVA and the goat anti-rabbit igg of CAP antibody are engaged on the liner plate successively, form chloromycetin immuomagnetic bead chromatographic test strip;
6) sample detection: the test strip sample pad is inserted testing sample solution, and sample will flow through from test strips by the chromatography effect, take out test strips after 10～20 seconds, after 15 minutes test board be put into Enviso in reaction under the room temperature again
TMThe Magnetic Assay Reader of System detects, and this detector will change into the biologically signal of field signal and further export in the electric signal mode; Behind the drawing standard curve, the establishing criteria curve is obtained the content of testing sample.
The preparation of described immunomagnetic beads: with CAP polyclonal antibody and nanometer magnetic bead coupling, preparation contains the immunomagnetic beads of CAP antibody: draw an amount of magnetic bead to small test tube, the centrifugal magnetic bead that makes separates with stock solution, remove stock solution and add ethyl sulfonic acid MES cleaning buffer solution vortex and ultrasonic cleaning and centrifugal, repeat several times, last resuspended magnetic bead, the water-soluble carbodiimide EDC and the N-hydroxy-succinamide NHS of the fresh configuration of certain volume are added activated carboxyl in the magnetic bead suspension, the mol ratio that makes magnetic bead surfaces carboxyl and EDC and NHS is 1: 4: 3, after the vortex mixed in room temperature stirring at low speed incubation reaction 2 hours, clean magnetic bead with MES cleaning buffer solution and boric acid cleaning buffer solution respectively, subsequently CAP antibody slowly being added dropwise in the magnetic bead suspension stirring at low speed incubation reaction spends the night, dropwise add mass concentration 5%BSA again, the final concentration that makes BSA is 1%, continue to stir 5min, with saturated free magnetic bead; Low-speed centrifugal is removed the magnetic bead precipitation of cohesion, and then cleans magnetic bead with the boric acid cleaning buffer solution and remove unconjugated protein, and magnetic bead is transferred to new pipe, discards the boric acid cleaning buffer solution, and uses the coupling buffer resuspension, puts 4 ℃ of refrigerators and deposits; Time spent then will be marked with the magnetic bead adding magnetic mark pad of CAP antibody with quantitative sample adding device, and will put into 37 ℃ of standby composition test strip of oven drying 10min.
Beneficial effect of the present invention: the present invention be based on a kind of nanometer magnetic immunological technique carry out the detection method of chloromycetin, it have highly sensitive, reaction time short, instrument and equipment is cheap, convenient characteristics such as easy-to-use.
Description of drawings
The Electronic Speculum figure of Fig. 1, magnetic bead.
Fig. 2, immunomagnetic beads reagent strip structural representation.
The magnetic signal of Fig. 3, positive detects synoptic diagram.The highest spike is represented detection signal, and the second high spike is represented the blank signal.
The canonical plotting of magnetic signal intensity when Fig. 4, chloromycetin 5～200ppb.
Embodiment
Make the immunomagnetic beads test strip of chloromycetin, at first need to prepare coupling chloromycetin carrier protein, be used to prepare relevant detection line (T line) and antibody; And need preparation chloromycetin magnetic mark antigen, be used to prepare corresponding magnetic mark antigen cellucotton; Need to prepare goat anti-rabbit igg antibody in addition, be used to prepare control line (C line).
1) the synthetic and evaluation of the holoantigen of chloromycetin: earlier chloromycetin is dissolved in N, in dinethylformamide (DMF) solution, adopt the mixed anhydride method synthetic antigen, and use bovine serum albumin(BSA) BSA as the immunizing antigen coupling carrier, ovalbumin OVA is as the envelope antigen coupling carrier, obtain the holoantigen CAP-BSA and the CAP-OVA of chloromycetin respectively, and utilize ultraviolet scanner to identify;
2) Polyclonal Antibody Preparation and purifying: with CAP-BSA as immunogene, adopt the animal of new zealand white rabbit as the quilt immunity, Freund's complete adjuvant with immunogenic and equivalent during first immunisation is mixed and made into emulsifying agent, at the subcutaneous multi-point injection in White Rabbit back, immunizing dose is 1mg/, and 2-4 is after week at interval, add the equivalent incomplete Freund with the same dose immunogenic and be mixed and made into emulsifying agent, booster immunization, monitoring antibody titer and specificity between duration of immunity, last immunity does not add adjuvant.Last immunity heart bloodletting after 7 days gets the CAP polyclonal antibody of purifying by fractional precipitation through sulfuric acid.And with the ammonium sulfate precipitation method antibody purification; Obtain the IgG of the anti-CAP-BSA of rabbit.
3) preparation of immunomagnetic beads: with CAP polyclonal antibody and nanometer magnetic bead coupling, preparation contains the immunomagnetic beads of CAP antibody; Concrete grammar is: draw an amount of magnetic bead to small test tube, the centrifugal magnetic bead that makes separates with stock solution, remove stock solution and add ethyl sulfonic acid (MES) cleaning buffer solution vortex and ultrasonic cleaning and centrifugal, repeat several times, last resuspended magnetic bead, the water-soluble carbodiimide (EDC) and the N-hydroxy-succinamide (NHS) of the fresh configuration of certain volume are added activated carboxyl in the magnetic bead solution, the mol ratio that makes magnetic bead surfaces carboxyl and EDC and NHS is 1: 4: 3, shook incubation reaction 2 hours in room temperature after the vortex mixed, clean magnetic bead with MES cleaning buffer solution and boric acid cleaning buffer solution respectively, subsequently CAP antibody slowly being added dropwise to the magnetic bead suspension the stirring at low speed incubation reaction spends the night, dropwise add 5%BSA again, the final concentration that makes BSA is 1%, continues to stir 5min, with saturated free magnetic bead.Low-speed centrifugal is removed the magnetic bead precipitation of cohesion, and then clean magnetic bead with the boric acid cleaning buffer solution, it is high speed centrifugation, keep the flowable furvous precipitation in bottom and remove the supernatant that contains unconjugated protein, repeat several times, preferably magnetic bead is transferred to new pipe, discard the boric acid cleaning buffer solution, and use the coupling buffer resuspension, put 4 ℃ of refrigerators and deposit.Time spent then will be marked with the magnetic bead adding magnetic mark pad of CAP antibody with quantitative sample adding device, and it is standby to form test strip to put into 37 ℃ of oven drying 10min.
4) bag is anti-to nitrocellulose filter (NC film) by CAP-OVA antigen and goat-anti rabbit two: with spray film instrument certain density CAP-OVA and the two anti-sprays respectively of goat-anti rabbit are stated from the detection line (T line) and control line (C line) of nitrocellulose filter (NC film), 37 ℃ of oven drying 10min are standby.
5) making of test strip: coupling pad, embedding antigen and two anti-nitrocellulose filter (NC film), sample pad, adsorptive pads, coverlay, the test board wild cards of respectively magnetic being marked the CAP polyclonal antibody are formed test strip (similar to the gold test strip bar).
6) sample detection: the sample end of chloromycetin immuomagnetic bead chromatographic test strip is inserted in the analyte sample fluid, insertion depth is no more than mark line, sample will flow through from test strips by the capillary chromatography effect, take out test strips after 10～20 seconds, after at room temperature reacting 15 minutes, test board is put into Enviso
TMThe Magnetic Assay Reader of System detects, and this detecting instrument can further be exported the biologically signal that changes into field signal in the mode of electric signal; Behind the drawing standard curve, the establishing criteria curve is obtained the occurrence of chloromycetin content in the testing sample.The detection that obtains this method is limited to about 6ppb.
Embodiment 1:
The detection of chloromycetin CAP in the aquatic products: take by weighing 5g sample after crushed to the 50mL centrifuge tube, the vibration of 80% acetonitrile solution vortex and then the ultrasonic Extraction that add 15mL, add the dissolving of 1g sodium chloride at last, and with the centrifugal 10min of 3000rpm, extract the upper organic phase clear liquid, dry up with nitrogen then.Residue after drying up with the damping fluid dissolving more at last obtains testing sample solution.Final with chloromycetin immuomagnetic bead chromatographic test strip test sample liquid, insertion depth is no more than mark line, and sample will flow through from test strips by the capillary chromatography effect, take out test strips after 10～20 seconds, after at room temperature reacting 15 minutes, test strip is put into Enviso
TMThe Magnetic Assay Reader of System detects field signal.The content of obtaining chloromycetin in the aquatic products sample to be measured according to the prior typical curve of measuring with the chloromycetin standard items is 9ppb.
Claims (3)
1, a kind of chloromycetin immuomagnetic bead chromatographic test strip, it is characterized in that forming by liner plate and the sample pad that on liner plate, is connected successively, magnetic mark pad, coated film, adsorptive pads, magnetic mark pad is the glass fibre cotton of absorption chloromycetin magnetic labeling antibody, the stealthy detection line T of the orthoscopic that the carrier protein solution of useful chloromycetin coupling on coated film is printed line is with the stealthy control line C of the orthoscopic line of goat anti-rabbit igg solution printing;
Hard plastic bar or the cardboard bar of described liner plate for not absorbing water; Sample pad is glass fibre cotton, nylon membrane, PVDF membrane or polyester film; Coated film is nitrocellulose filter, pure cellulose film or carboxylation cellulose membrane; Adsorptive pads is absorbent filter or filter paper for oil;
Described chloromycetin magnetic labeling antibody is the polyclonal antibody of the chloromycetin of marked by magnetic bead;
Described magnetic bead is the Fe that finishing has carboxyl
3O
4Magnetic bead;
The carrier protein of described coupling chloromycetin is: bovine serum albumin(BSA) BSA, the pure albumen OVA of ovum gallinaceum.
2, the preparation method of the described chloromycetin immuomagnetic bead chromatographic of claim 1 test strip is characterized in that
1) the synthetic and evaluation of chloromycetin complete antigen: adopt mixed anhydride method respectively with BSA in DMF solution, OVA and chloromycetin CAP carry out coupling, obtain the holoantigen CAP-BSA and the CAP-OVA of chloromycetin, and utilize ultraviolet scanner to identify;
2) Polyclonal Antibody Preparation and purifying: as immunogene, immune new zealand rabbit prepares the CAP polyclonal antibody and according to a conventional method with the ammonium sulfate purifying with CAP-BSA;
3) preparation of immunomagnetic beads: with the nanometer magnetic bead coupling of CAP antibody with the band carboxyl, preparation contains the immunomagnetic beads of CAP antibody, i.e. chloromycetin magnetic labeling antibody; Prepare magnetic mark pad with glass fibre cotton absorption chloromycetin magnetic labeling antibody;
4) embedding CAP-OVA and goat anti-rabbit igg are to coated film: the coating antigen CAP-OVA spray that will select concentration respectively with spray film instrument is stated from the T line of coated film, and the goat anti-rabbit igg spray is stated from the C line of coated film, and 37 ℃ of oven drying 10min are standby;
5) making of test strip: coated film, the adsorptive pads of sample pad, magnetic being marked pad, embedding coating antigen CAP-OVA and the goat anti-rabbit igg of CAP antibody are engaged on the liner plate successively, form chloromycetin immuomagnetic bead chromatographic test strip;
6) sample detection: the test strip sample pad is inserted testing sample solution, and sample will flow through from test strips by the chromatography effect, take out test strips after 10～20 seconds, after 15 minutes test board be put into Enviso in reaction under the room temperature again
TMThe Magnetic Assay Reader of System detects, and this detector will change into the biologically signal of field signal and further export in the electric signal mode; Behind the drawing standard curve, the establishing criteria curve is obtained the content of testing sample.
3, preparation method according to claim 2, it is characterized in that the preparation of described immunomagnetic beads: with CAP polyclonal antibody and nanometer magnetic bead coupling, preparation contains the immunomagnetic beads of CAP antibody: draw an amount of magnetic bead to small test tube, the centrifugal magnetic bead that makes separates with stock solution, remove stock solution and add ethyl sulfonic acid MES cleaning buffer solution vortex and ultrasonic cleaning and centrifugal, repeat several times, last resuspended magnetic bead, the water-soluble carbodiimide EDC and the N-hydroxy-succinamide NHS of the fresh configuration of certain volume are added activated carboxyl in the magnetic bead suspension, the mol ratio that makes magnetic bead surfaces carboxyl and EDC and NHS is 1: 4: 3, after the vortex mixed in room temperature stirring at low speed incubation reaction 2 hours, clean magnetic bead with MES cleaning buffer solution and boric acid cleaning buffer solution respectively, subsequently CAP antibody slowly being added dropwise in the magnetic bead suspension stirring at low speed incubation reaction spends the night, dropwise add mass concentration 5%BSA again, the final concentration that makes BSA is 1%, continues to stir 5min, with saturated free magnetic bead; Low-speed centrifugal is removed the magnetic bead precipitation of cohesion, and then cleans magnetic bead with the boric acid cleaning buffer solution and remove unconjugated protein, and magnetic bead is transferred to new pipe, discards the boric acid cleaning buffer solution, and uses the coupling buffer resuspension, puts 4 ℃ of refrigerators and deposits; Time spent then will be marked with the magnetic bead adding magnetic mark pad of CAP antibody with quantitative sample adding device, and will put into 37 ℃ of standby composition test strip of oven drying 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100274585A CN101551391A (en) | 2009-05-07 | 2009-05-07 | Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100274585A CN101551391A (en) | 2009-05-07 | 2009-05-07 | Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101551391A true CN101551391A (en) | 2009-10-07 |
Family
ID=41155747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009100274585A Pending CN101551391A (en) | 2009-05-07 | 2009-05-07 | Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101551391A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043059A (en) * | 2009-10-26 | 2011-05-04 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Two methods for preparing immunogen and coating antigen used for detecting chloramphenicol |
| CN102169120A (en) * | 2010-02-25 | 2011-08-31 | 艾博生物医药(杭州)有限公司 | Detector |
| CN102353774A (en) * | 2011-06-13 | 2012-02-15 | 清华大学深圳研究生院 | Immunochromatographic test paper for detecting chloramphenicol and its preparation method |
| CN107782900A (en) * | 2016-08-31 | 2018-03-09 | 朱海燕 | The Test paper component of human growth and differentiation factor 7 15 |
| CN112730857A (en) * | 2020-12-14 | 2021-04-30 | 上海海洋大学 | Magnetic immunochromatographic test strip and method for rapidly detecting MS-222 |
-
2009
- 2009-05-07 CN CNA2009100274585A patent/CN101551391A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043059A (en) * | 2009-10-26 | 2011-05-04 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Two methods for preparing immunogen and coating antigen used for detecting chloramphenicol |
| CN102169120A (en) * | 2010-02-25 | 2011-08-31 | 艾博生物医药(杭州)有限公司 | Detector |
| CN102353774A (en) * | 2011-06-13 | 2012-02-15 | 清华大学深圳研究生院 | Immunochromatographic test paper for detecting chloramphenicol and its preparation method |
| CN107782900A (en) * | 2016-08-31 | 2018-03-09 | 朱海燕 | The Test paper component of human growth and differentiation factor 7 15 |
| CN112730857A (en) * | 2020-12-14 | 2021-04-30 | 上海海洋大学 | Magnetic immunochromatographic test strip and method for rapidly detecting MS-222 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101561437A (en) | Immunity bead chromatograph test strip for quick testing ractopamine and preparation method thereof | |
| CN101551390A (en) | Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof | |
| US20070059682A1 (en) | Method to increase specificity and/or accuracy of lateral flow immunoassays | |
| US20140322724A1 (en) | Homogeneous competitive lateral flow assay | |
| CN101000343A (en) | Immunological test element with improved control zone | |
| CN109765384A (en) | A kind of canine coronavirus antibody fluorescence test strip and its preparation method and application | |
| CN101165491A (en) | Method for detecting double-chain DNA resistant antibody using gold magnetic particle as carrier | |
| CN104198716A (en) | Animal brucella antibody gold mark rapid detection kit | |
| CN101551391A (en) | Immuomagnetic bead chromatographic test strip for rapidly detecting chloromycetin and preparation method thereof | |
| CN107271682A (en) | A kind of dog c reactive protein fluorescence detection test strip | |
| CN102135541A (en) | Detection method of enterobacter sakazakii and gold-labeled rapid diagnosis kit for same and preparation method thereof | |
| CN113156105A (en) | A type botulinum toxin rapid quantitative detection card | |
| CN109856406A (en) | A kind of canine parvovirus antibody fluorescence test strip and its preparation method and application | |
| CN109459570A (en) | The colloid gold immune test paper preparation method of lead ion in a kind of quick detection blood of human body | |
| CN102565382A (en) | Immunochromatography method for detecting allergen-specific IgE antibodies in blood samples | |
| CN109164257A (en) | A kind of time-resolved fluoroimmunoassay detection method based on magnetic particle | |
| JP2003215126A (en) | Method for extracting microbe antigen | |
| JP2008051806A (en) | USE OF ALBUMIN, BOVIN, P-AMINOPHENYL N-ACETYL beta-D GLUCOSAMINIDE FOR IMMUNOASSAY DEVICE AS CONTROL LINE | |
| CN105353116A (en) | Method for immunoassay based on hydrogen peroxide test strip and applications | |
| CN100357739C (en) | Test paper strip for semi-quantitative detecting Heterosigma akashiwo, its preparation and using method | |
| CN206038688U (en) | Immunity chromatography detection test strip's fluorescent quantitation spectral detection system | |
| CN109975538A (en) | A kind of detection perlsucht IFN-γ and the test strips of bovine paratuberculosis antibody and preparation method thereof | |
| JPH07140144A (en) | Method for measuring allergen specific ige antibody and antigen-antibody complex | |
| CN104198692A (en) | Mixed marking substance and marking method as well as application thereof | |
| CN101482565A (en) | Melamine colloidal gold immunochromatography detection test paper and its production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20091007 |